CN206580839U - A kind of enclosed type electric transfers from one department to another system - Google Patents

Abstract

The utility model mainly provides a kind of enclosed electricity and transfers from one department to another system.Electric turn trough, cell feeding mechanism and cell retracting device are connected by connecting tube and closed system is formed；Circulated with drive device driving liquid between cell feeding mechanism and electric turn trough and between cell retracting device and electric turn trough.Type of drive can be divided into manually operated and automation mechanized operation.The system is much cleaner safer compared with open electricity transfers from one department to another system, while reducing the hygienic rank of working environment, the electricity that can be widely applied to clinically cultured cell in vitro turns.

Description

A kind of enclosed type electric transfers from one department to another system

Technical field

The utility model is related to a kind of control device, and particularly a kind of electricity of Clinical practice turns the control device and electricity of cell Rotary device；Belong to medical apparatus and instruments technical field.

Background technology

It is a kind of wide variety of experimental technique in biology that electricity turn, which is,.It is mainly used in DNA, RNA and protein nucleic acid Compound etc. imported into intracellular.During electricity turns, highfield causes electrically charged cell membrane to produce hole, due to DNA, RNA and nucleic acid-protein complex are all negatively charged, towards positive pole movement in electricity transfers from one department to another the electric field that system applies, therefore DNA, RNA and nucleic acid-protein complex can enter intracellular by the cell membrane cavity temporarily opened.In general electricity turns setting, carefully The time that after birth cavity is opened is very short, therefore electricity turns over journey and injured for cell less.

The general step that electricity turns includes：Suspension cell；Wash cell；Centrifugal concentrating cell；Cell is resuspended in less volume And mix the DNA, RNA and protein nucleic acid compound etc. for needing to import；Mixture is put into electricity and transfers from one department to another system；Start electricity to turn；Finally Reclaim cell.Existing electricity, which transfers from one department to another system, includes BTX830, and Biorad genepulser, Neon systems etc. are required for cell to add Enter or be drawn into electricity to transfer from one department to another in system, this process allows cell to be exposed in air.During electricity turns, the cell of in vitro culture The external world can be touched, whole process is not in the system of a closing, therefore referred to as open electricity transfers from one department to another system.Open electricity General requirement of experiment can be met by transfering from one department to another system, but clinically, be frequently encountered the problem of electricity turns the cell of in vitro culture. Such as production mRNA CART cells are, it is necessary to which electricity turns CAR mRNA in T cell.Open electricity transfers from one department to another system because cell is exposed to The external world, therefore systemic contamination is very risky, it is impossible to meet clinical demand.In addition, existing electricity transfers from one department to another to unite and is only satisfied with The electricity of small scale experiments rank turns, the design of electric turn trough it is smaller, the cell quantity that electricity turns every time is limited, and one in clinical production As be all large-scale production process.Therefore existing electricity, which transfers from one department to another system, can not all meet clinical demand.

The content of the invention

The utility model transfers from one department to another the deficiency of system for existing open electricity, on the one hand provides a kind of enclosed type electric turn trough, Including outside support, electrode 34 and electric turn trough lid 32, also comprising at least one sample channel 33, the sample channel passes through joint 31 and external connection, the joint 31 is arranged on the electric turn trough and covered, and the connected mode of the joint and outside is close Envelope connection, preferably described joint 31 is Luer joints.

It is preferred that in order to prevent electricity turn before cell and electricity turn after mixing with cells, described enclosed type electric turn trough can be with Comprising two sample channels 33, one is used for sample introduction, and one is used to reclaim cell；Preferably, the sample channel 33 is set respectively Put in the both sides of the electric turn trough lid 32, or described two sample channels 33 are concurrently arranged on the electric turn trough lid 32 Side.

In order to obtain more passages for other purposes, for example, wash and collect waste liquid；The enclosed type electric turn trough can To set more sample channels, for example, it is provided with two parallel sample channels 33 respectively in the both sides of electric turn trough lid 32.

Above-mentioned electric turn trough meets enclosed type electric and turns to require to turn cell requirement with extensive electricity.Design advantages of simple ingenious, into This is relatively low.

On the other hand the utility model provides a kind of enclosed type electric and transfers from one department to another system, comprising above-mentioned any electric turn trough, and also Including cell feeding mechanism 1, cell retracting device 2, connecting tube 4 and drive device 6；The cell feeding mechanism 1, cell are returned Receiving apparatus 2 and electric turn trough 3 are interconnected by connecting tube 4, and form closed system；The drive device 6 drives liquid thin Circulated between born of the same parents' feeding mechanism 1 and electric turn trough 3 and between cell retracting device 2 and electric turn trough 3.

Electricity can be drawn in practical operation with syringe to turn in sample injection cell feeding mechanism and mix, then by driving Cell suspension in cell feeding mechanism is imported into electricity in electric turn trough and turned by dynamic device, after then electricity is turned by drive device Cell liquid is transferred in cell retracting device.Due to system lock, cell is in sterile closing all the time during whole electricity turns Environment, be not directly contacted with the external world.Therefore reduce the dangerous of pollution, be conveniently used for clinic.

It is preferred that to simplify pipeline, described enclosed type electric, which transfers from one department to another system, also includes three-way control valve 5；The cell supply Device 1, cell retracting device 2 and electric turn trough 3 are connected by three interfaces of connecting tube and the three-way control valve 5 respectively, institute Drive device 6 is stated to be arranged between the three-way control valve 5 and the electric turn trough 3.

It is highly preferred that transfering from one department to another system to provide a kind of suitable manually operated enclosed type electric, the drive device 6 can be note Emitter combining structure, the combinations of syringe structure includes the flow direction controller 62 of syringe 61 and first, first flow direction Controller 62 includes at least three adjustable interfaces of switch, and an interface of the flow direction controller 62 of syringe 61 and first connects Connect；The three-way control valve 5 and electric turn trough 3 are connected by other interfaces of connecting tube and first flow direction controller 62 respectively Connect, by manual aspirating syringe 61 liquid can be driven to be circulated in pipeline.

Turn possible pollution during sample transfer operation to reduce electricity, it is preferable that described enclosed type electric transfers from one department to another system Also include sampling syringe needle 7, and first flow direction controller 62 includes 4 interfaces, wherein 3 joints are provided with unidirectional regulating valve And be connected respectively with the three-way control valve 5, electric turn trough 3 and sampling syringe needle 7, another 1 joint and syringe 61 are connected.With Sampling needle aspirate electricity turns sample, then injects cell feeding mechanism by syringe, carries out electricity after mixing again and turns operation.

It is also preferable that described enclosed type electric, which transfers from one department to another system, also includes the second flow direction controller 63 and sampling syringe needle 7；It is described Second flow direction controller 63 includes at least three adjustable joints of switch, and one with first flow direction controller 62 respectively Interface, syringe 61 and sampling syringe needle 7 are connected.

In addition, in order to realize that continuous electricity turns, described enclosed type electric transfer from one department to another system can also include waste collecting device 8 and Clean liquid supply unit 9；The electric turn trough 3 includes four sample channels, and four sample channels pass through connecting tube and cell respectively Feeding mechanism 1, cell retracting device 2, waste collecting device 8 and cleaning liquid supply unit 9 are connected, and form closed system； The drive device 6 driving liquid between cell feeding mechanism 1 and electric turn trough 3, waste collecting device 2 and electric turn trough 3 it Between, circulate between cleaning liquid supply unit 8 and electric turn trough 3 and between cell retracting device 9 and electric turn trough 3.After electricity turns Electric turn trough can be easily cleaned, so as to repeatedly utilize enclosed type electric turn trough, experiment material is saved, has saved cost, is protected The high efficiency of experiment is demonstrate,proved.

It is highly preferred that the drive device is peristaltic pump.Most preferably, between cell feeding mechanism 1 and electric turn trough 3, Between waste collecting device 2 and electric turn trough 3, between cleaning liquid supply unit 8 and electric turn trough 3 and in cell retracting device 9 Independent peristaltic pump is respectively provided between electric turn trough 3.

The main advantage of the system have it is following some：1st, the system is totally enclosed type system.Cell turns over journey in whole electricity In all the time be in sterile closing environment, be not directly contacted with the external world.Therefore reduce the dangerous of pollution.

2nd, the system is easy to operate.The system can control controlling flow direction by adjusting multiple rotary switches.The system is grasped Make step simple, a few minutes can complete electricity and turn over journey.

3rd, the DNA that cell concentration turns in smaller volume, electricity during electricity turns, RNA or protein nucleic acid compound In the volume of very little.General cell manipulation system pipeline length and thick, liquid dead volume causes electricity to turn the wave of material than larger Take, therefore the requirement that clinical electricity turns can not be met.In the system, pipeline system design is thin and short, substantially reduces liquid dead volume Product, meets the requirement that clinical electricity turns.

4th, the system can be applied to larger scale clinical production.The system electricity turn trough size can amplify, electric to increase once The cell quantity turned.The Automation Design can realize that continuous several times electricity turns simultaneously, therefore substantially increase accessible sample number Amount.It is adapted to the cell that extensive electricity turns in vitro culture.

5th, the conventional electricity of electric turn trough and BTX830 square waveform systems etc. turns system compatible, and electroporation can be used Harvard apparatus company BTX830 or similar system.Therefore can wide popularization and application.

Brief description of the drawings

Fig. 1 e-1f are the structural representation according to enclosed type electric turn trough of the present utility model, wherein, Fig. 1 a are according to this reality With a kind of structural perspective of new electric turn trough for possessing single sample passage；Fig. 1 b are the structural side view of the electric turn troughs of Fig. 1 a.

Fig. 1 c are according to a kind of structural perspective for the electric turn trough for possessing two sample channels of the present utility model；

Fig. 1 d are the structural side view of the electric turn troughs of Fig. 1 c.

Fig. 1 e are according to a kind of structural perspective for the electric turn trough for possessing four sample channels of the present utility model；

Fig. 1 f are the structural side view of the electric turn troughs of Fig. 1 e.

Fig. 2 is the structural representation for transfering from one department to another system according to a kind of enclosed type electric of the present utility model.

Fig. 3 is the structural representation for transfering from one department to another system according to another enclosed type electric of the present utility model.

Fig. 4 is the structural representation for transfering from one department to another system according to another enclosed type electric similar with Fig. 3 of the present utility model.

Fig. 5 is the structural representation for transfering from one department to another system according to a kind of automation enclosed type electric of the present utility model.

Fig. 6 is the structural representation for transfering from one department to another system according to another automation enclosed type electric of the present utility model.

Mark：1-cell feeding mechanism；2-cell retracting device；3-electricity turn trough；31-electricity turn trough joint；32-electricity Turn trough lid；33-sample channel；3-electrode；4-connecting tube；5-three-way control valve；6-drive device；61-syringe； 62-the first flow direction controller；63-the second flow direction controller；7-syringe needle；8-cleaning liquid supply unit；9-waste collection Device.

Embodiment

In order to deepen to understand of the present utility model, below in conjunction with the accompanying drawings with specific implementation case, this reality is expanded on further With new.

Embodiment 1

As shown in Fig. 1 e-1f, in a specific implementation according to electric turn trough of the present utility model, the electric turn trough is removed Outside plastic stent, mainly include three parts：Electric turn trough lid（With the interface being connected with luer connectors）, sample intake passage and aluminium Electrode.During sample introduction, luer connectors are connected with electric turn trough lid.

The embodiment of the electric turn trough of only one of which sample channel is shown in Fig. 1 a and 1b, and cell, which enters and reclaimed, all to be used The passage.The electric turn trough is that manual electricity turns Special electric turn trough.

What Fig. 1 c and 1d, Fig. 1 e and 1f were shown is that automation electricity turns electric turn trough.

The embodiment for the electric turn trough for having two sample channels is shown in Fig. 1 c and 1d, and described two sample channels are located at electricity The turn trough left and right sides.Left-hand channel is used for sample introduction, and right-side channels are used to reclaim cell.

The embodiment for the electric turn trough for having four sample channels is shown in Fig. 1 e and 1f, there is two groups of samples be arrangeding in parallel respectively Product passage is located at the electric turn trough left and right sides.Two sample channels in left side are respectively used to cell and cleaning buffer solution sample introduction.Right side Two passages are respectively used to reclaim cell and discharge cleaning buffer solution.And electric turn trough its sample channel that Fig. 1 e and 1f are shown will It is wider than the electric turn trough that Fig. 1 c and 1d are shown.

Embodiment 2

It is the embodiment for transfering from one department to another system according to an enclosed type electric of the present utility model as shown in Figure 2.

The enclosed type electric, which transfers from one department to another system, includes cell feeding mechanism 1, cell retracting device 2, electric turn trough 3, connecting tube 4, threeway Regulating valve 5, the flow direction controller 62 of syringe 61 and first；First flow direction controller 62 has three interfaces, and switchs adjustable. Syringe 61 is connected with an interface of the first flow direction controller 62；Electric turn trough 3 is by connecting tube 4 with the first flow direction controller 62 Another interface；First flow direction controller 62 also has an interface connection to be connected with a joint of three-way control valve 5, threeway Two other joint of regulating valve 5 is distinguished cell feeding mechanism 1, cell retracting device 2 by connecting tube 4 and is interconnected.It is whole Individual internal system closing.

The enclosed type electric, which transfers from one department to another system, needs exclusive use needle and syringe head absorption electricity to turn to inject a sample into cell after sample Feeding mechanism, makes sample and mixing with cells.After sample and mixing with cells, electricity can be followed the steps below and turned：1st, draw thin Born of the same parents；2nd, cell is imported into electric turn trough；3rd, electricity turns；4th, cell is drawn to syringe；Cell is injected cell retracting device by 5.

Concrete operations are as follows:Using syringe and syringe needle from the sample bottle of closing pipette samples.Syringe needle is removed, will be injected Device is connected to cell feeding mechanism.By cell feeding mechanism sample holes, cell feeding mechanism is injected a sample into.Gently squeezed with hand Pressure shakes kinetocyte feeding mechanism to mix cell.Cell feeding mechanism 1 and electric turn trough 3 are connected by connecting tube 4.Threeway is adjusted to adjust The flow direction controller 62 of valve 5 and first is saved, makes cell feeding mechanism 1 and the pipeline of syringe 61 unimpeded, cell retracting device 1 is closed With syringe 61, the connection between electric turn trough 3 and syringe 61.The thin of certain volume is drawn according to requirement of experiment using syringe 61 Born of the same parents and sample suspension.The first flow direction controller 62 is adjusted, makes electric turn trough 3 and the pipeline of syringe 61 unimpeded, cell supply dress is closed Put 1 and syringe 3, the connection between cell retracting device 2 and syringe 61.The cell and sample of absorption are hanged using syringe 61 Liquid is slowly injected into electric turn trough 3 as shown in Figure 1a.The sample channel that sample and cell suspension are embedded by electric turn trough 3 enters Electric turn trough.Setting electricity turns parameter, puts electric turn trough into electroporation.Start electricity to turn.After the completion of electricity turns, inhaled again using syringe 61 Sample and cell suspension in power taking turn trough 3.The flow direction controller 62 of three-way control valve 5 and first is adjusted, cell is reclaimed dress Put 2 and the pipeline of syringe 61 it is unimpeded, close cell feeding mechanism 1 and syringe 61, the connection between electric turn trough 3 and syringe 61. Pallium cell injection is entered into cell retracting device 2 using syringe 61.Electricity turns to complete.

Embodiment 3

It is the embodiment for transfering from one department to another system according to another enclosed type electric of the present utility model as shown in Figure 3.

The enclosed type electric, which transfers from one department to another system, includes cell feeding mechanism 1, cell retracting device 2, electric turn trough 3, connecting tube 4, threeway Regulating valve 5, syringe 61, the first flow direction controller 62 and sampling syringe needle 7.First flow direction controller 62 includes 4 interfaces, its In 3 joints be connected provided with unidirectional regulating valve and respectively with the three-way control valve 5, electric turn trough 3 and sampling syringe needle 7, another 1 Joint and syringe 61 are connected.Two other joint of three-way control valve 5 distinguishes cell feeding mechanism 1 by connecting tube 4, thin Born of the same parents' retracting device 2 is interconnected.Closed inside whole system.

The enclosed type electric transfer from one department to another system need not be used alone needle and syringe head draw electricity turn sample, it is convenient and swift, it is to avoid Multiple pipeline connection and win.Electricity turn can be followed the steps below by transfering from one department to another system using the enclosed type electric：1st, pipette samples； 2nd, cell is drawn；3rd, cell is imported into electric turn trough；4th, electricity turns；5th, cell is drawn to syringe；6th, cell injection cell is returned Receiving apparatus.

Its concrete operations is as follows：The single-way switch between syringe 61 and syringe needle 7 is opened, syringe is simultaneously closed off 61 and cell feeding mechanism 1, the single-way switch between syringe 61 and the electric turn trough 3 of closing.Using syringe 61 and syringe needle 7 from envelope Pipette samples in the sample bottle closed.The single-way switch closed between syringe 61 and syringe needle 7, opens syringe 61 and thin The single-way switch of born of the same parents' feeding mechanism 1, while adjusting the flow direction controller 62 of three-way control valve 5 and first, makes cell feeding mechanism 1 Pipeline between syringe 61 is unimpeded, the pipeline closed between cell retracting device 2 and syringe 61.Use syringe 61 The cell of certain volume is drawn according to requirement of experiment.The first flow direction controller 62 is adjusted, makes electric turn trough 3 and the pipeline of syringe 61 smooth It is logical, close the single-way switch of cell feeding mechanism 1 and syringe 61.The cell and sample suspension of absorption are delayed using syringe 61 In the slow electric turn trough 3 being injected into as shown in Figure 1a.The sample channel that sample and cell suspension are embedded by electric turn trough 3 enters electricity and turned Groove 3.Setting electricity turns parameter, puts electric turn trough into electroporation.Start electricity to turn.After the completion of electricity turns, drawn again using syringe 61 Sample and cell suspension in electric turn trough.The first flow direction controller 62 is adjusted, unidirectionally opening between electric turn trough 3 and syringe 61 is closed Close, open the single-way switch of cell retracting device 2 and syringe 61, while adjusting three-way control valve 5, make cell retracting device 2 Keep unimpeded with the pipeline of syringe 61, close cell feeding mechanism 1 and be connected with the pipeline of syringe 61.Will using syringe 61 Pallium cell injection enters cell retracting device 2.Electricity turns to complete.

Fig. 4 shows that another enclosed type electric similar with Fig. 3 transfers from one department to another system.Difference is to employ different open Close and set.Fig. 3 enclosed type electrics transfer from one department to another system and have used the first flow direction controller and a three-way control valve, and Fig. 4 enclosed type electrics transfer from one department to another System has used three groups of three-way control valves.Two kinds of design operation modes are similar, will not be described here.

Embodiment 4

It is the embodiment for transfering from one department to another system according to one of the present utility model automation electricity as shown in Figure 5.

The enclosed type electric, which transfers from one department to another system, includes cell feeding mechanism 1, cell retracting device 2, electric turn trough 3, connecting tube and drive Dynamic device 6, drive device is preferably peristaltic pump；Cell feeding mechanism 1, cell retracting device 2 pass through connecting tube and electricity respectively Turn trough 3 is connected, and is all provided between cell feeding mechanism 1 and electric turn trough 3 and between cell retracting device 2 and electric turn trough 3 It is equipped with drive device 6.Closed inside whole system.

Journey can be turned over by the whole electricity of Automated condtrol in closed system by the system.With two shown in Fig. 3 and Fig. 4 The difference that electricity transfers from one department to another system is planted, the system is not to provide liquid mobilization dynamic by syringe, but passes through electronically controlled wriggle Pump provides liquid mobilization dynamic.The basic operational steps for transfering from one department to another system using the enclosed type electric include：1st, cell is passed through into peristaltic pump Import electric turn trough；2nd, electricity turns；3rd, cell will be injected by cell retracting device by peristaltic pump.In the enclosed type electric transfers from one department to another system, Need exclusive use needle and syringe head to draw electricity to turn to inject a sample into cell feeding mechanism after sample, make sample and cell mixed Close.

Its concrete operation step is as follows.Peristaltic pump is opened, by peristaltic pump by cell sample suspension from cell feeding mechanism 1 In imported into electric turn trough 3 as illustrated in figure 1 c.The sample channel that sample and cell suspension are embedded by electric turn trough 3 enters electricity and turned Groove.Close peristaltic pump.Setting electricity turns parameter, puts electric turn trough into electroporation.Start electricity to turn.After the completion of electricity turns, peristaltic pump is opened Two, the cell that electricity turns to complete is imported into cell retracting device 2.Electricity turns to complete.Electric turn trough 3 can be typically reused several times. But it is due to electrode after electricity turns, might have sample or pellet cell debris, the follow-up electricity of influence turns.In the present system, can be with Pass through the electric turn trough of Luer interfaces quick-replaceable.Electric turn trough has been changed, electricity can be completed by similar steps and turned.

Embodiment 5

It is the embodiment for transfering from one department to another system according to another automation electricity of the present utility model as shown in Figure 6.Turn in electricity shown in Fig. 5 Cleaning liquid supply unit 8 and waste liquid recovery apparatus 9 are added on the basis of system, combination transfers from one department to another system with electricity shown in Fig. 5 It is similar, repeat no more.Cleaning fluid can be connected after the completion of individually electricity turns every time, electric turn trough is cleaned by Electronic Control.Cleaning After the completion of continuously can complete repeatedly electricity using same electric turn trough and turn.This design is simple ingenious, has saved electric turn trough and has used, together When avoid and repeatedly change electric turn trough.The enclosed type electric transfers from one department to another system and uses the electric turn trough shown in Fig. 1 e, it is characterised in that broaden Electric turn trough embed sample channel.The basic operational steps for transfering from one department to another system using the enclosed type electric include：1st, by cell by wriggling Pump imports electric turn trough；2nd, electricity turns；3rd, cell will be injected by cell retracting device by peristaltic pump；4th, cleaning fluid is passed through into peristaltic pump It imported into electric turn trough；5, wait 1-2 minutes；6th, cleaning fluid is imported by waste fluid bag by peristaltic pump.Transfer from one department to another in the enclosed type electric Turn to inject a sample into cell feeding mechanism after sample, it is necessary to which needle and syringe head is used alone and draws electricity in system, make sample and thin Born of the same parents mix.

Its concrete operation step is as follows：Peristaltic pump one is opened, cell sample suspension is supplied from cell by peristaltic pump and filled Put and imported into 1 in electric turn trough 3 as shown in fig. le.The sample channel that sample and cell suspension are embedded by electric turn trough enters electricity Turn trough.Close peristaltic pump one.Setting electricity turns parameter, puts electric turn trough 3 into electroporation.Start electricity to turn.After the completion of electricity turns, open compacted Dynamic pump two, cell retracting device 2 is imported into by the cell that electricity turns to complete.Electricity turns to complete.Peristaltic pump three is opened, by cleaning fluid from clear It is imported into washing lotion feeding mechanism 8 in electric turn trough 3.Electrode is allowed to soak a few minutes in cleaning fluid.Peristaltic pump four is opened, will be cleaned Liquid is imported into waste liquid recovery apparatus 9.Electric turn trough 3 after cleaning may be reused.Therefore repeatedly continuous electricity it can turn.No Electric turn trough has only been saved, and has realized the large-scale electricity of high efficiency and has turned over journey.

It should be noted that above-described embodiment is only preferable embodiment of the present utility model.It is not used for limiting this reality With new protection domain.The equivalent substitute made on the basis of the embodiment above or replacement, belong to the utility model Protection domain.

Claims (10)

1. a kind of enclosed type electric turn trough, including outside support, electrode (34) and electric turn trough lid（32）, it is characterised in that also include At least one sample channel（33）, the sample channel passes through joint（31）With external connection, the joint（31）It is arranged on institute Electric turn trough is stated to cover, and the joint is to be tightly connected with outside connected mode.

2. enclosed type electric turn trough as claimed in claim 1, it is characterised in that include two sample channels（33）, the sample lead to Road（33）It is separately positioned on the electric turn trough lid（32）Both sides, or described two sample channels（33）Concurrently it is arranged on The electric turn trough lid（32）Side.

3. enclosed type electric turn trough as claimed in claim 1, it is characterised in that the electric turn trough lid（32）Both sides be all provided with respectively It is equipped with two parallel sample channels（33）.

4. a kind of enclosed type electric transfers from one department to another system, electric turn trough as claimed in claim 1 is included, it is characterised in that also supplied including cell Answer device（1）, cell retracting device（2）, connecting tube（4）And drive device（6）；The cell feeding mechanism（1）, cell return Receiving apparatus（2）With electric turn trough（3）Pass through connecting tube（4）It is interconnected, and forms closed system；The drive device（6）Driving Liquid is in cell feeding mechanism（1）With electric turn trough（3）Between and in cell retracting device（2）With electric turn trough（3）Between circulate.

5. enclosed type electric as claimed in claim 4 transfers from one department to another system, it is characterised in that also including three-way control valve（5）；The cell Feeding mechanism（1）, cell retracting device（2）With electric turn trough（3）Pass through connecting tube and the three-way control valve respectively（5）Three Interface is connected, the drive device（6）It is arranged on the three-way control valve（5）With the electric turn trough（3）Between.

6. enclosed type electric as claimed in claim 5 transfers from one department to another system, it is characterised in that the drive device（6）For combinations of syringe Structure, the combinations of syringe structure includes syringe（61）And first flow direction controller（62）, first traffic organising Device（62）Include at least three adjustable joints of switch, the syringe（61）With the first flow direction controller（62）An interface Connection；The three-way control valve（5）And electric turn trough（3）Pass through connecting tube and first flow direction controller respectively（62）Its He connects interface, passes through syringe（61）Suction driving liquid communication.

7. enclosed type electric as claimed in claim 6 transfers from one department to another system, it is characterised in that also including sampling syringe needle（7）, and described One flow direction controller（62）Comprising 4 interfaces, wherein 3 joints provided with unidirectional regulating valve and respectively with the three-way control valve （5）, electric turn trough（3）And sampling syringe needle（7）Connection, another 1 joint and syringe（61）Connection.

8. enclosed type electric as claimed in claim 6 transfers from one department to another system, it is characterised in that also including the second flow direction controller (63) and take Sample syringe needle（7）；Second flow direction controller（63）Comprising at least three adjustable joints of switch, and respectively with it is described first-class To an interface of controller (62), syringe (61) and sampling syringe needle (7) connection.

9. enclosed type electric as claimed in claim 4 transfers from one department to another system, it is characterised in that also including waste collecting device（8）And it is clear Washing lotion feeding mechanism（9）；The electric turn trough（3）Comprising four sample channels, four sample channels are respectively by connecting tube and thin Born of the same parents' feeding mechanism（1）, cell retracting device（2）, waste collecting device（8）And cleaning liquid supply unit（9）Connection, and formed Closed system；The drive device（6）Liquid is driven in cell feeding mechanism（1）With electric turn trough（3）Between, waste collection dress Put（8）With electric turn trough（3）Between, cleaning liquid supply unit（9）With electric turn trough（3）Between and in cell retracting device（2） With electric turn trough（3）Between circulate.

10. the enclosed type electric as described in claim 4,5 or 9 are any transfers from one department to another system, it is characterised in that the drive device is wriggling Pump.