CN204789592U - Glycated haemoglobin detect reagent box - Google Patents
Glycated haemoglobin detect reagent box Download PDFInfo
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- CN204789592U CN204789592U CN201520392455.2U CN201520392455U CN204789592U CN 204789592 U CN204789592 U CN 204789592U CN 201520392455 U CN201520392455 U CN 201520392455U CN 204789592 U CN204789592 U CN 204789592U
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- reagent
- glycosylated hemoglobin
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Abstract
The utility model relates to a glycated haemoglobin detects technical field, concretely relates to glycated haemoglobin detect reagent box, the kit includes the box body, lay in the inboard inside lining in bottom and place aluminium foil bag, reagent bottle, chromatography column and the capillary heparin tube on the inside lining, and the aluminium foil bag is built -in to be equipped with brown centrifuging tube, is equipped with first reagent in the brown centrifuging tube, is equipped with second reagent in the reagent bottle, and the chromatography column includes the shell and is fixed in the reaction membrane in the shell. The utility model discloses a kit only need micro whole blood live the tip blood specimen this, can realize quantitative determination glycated haemoglobin's content greatly having improved the speed of examination in 2 -3 minute, have sensitivity height, advantage that the specificity is good and easy and simple to handle.
Description
Technical field
The utility model relates to glycosylated hemoglobin detection technique field, is specifically related to a kind of glycosylated hemoglobin detection kit.
Background technology
Glycosylated hemoglobin detection method conventional clinically at present has: high performance liquid chromatography, immunization, enzyme process, ion-exchange chromatography, chemoluminescence method etc.High performance liquid chromatography, ion-exchange chromatography need to use large-scale instrument, and instrument price is expensive; Immunization needs to use lot of antibodies, and needs to use large-scale instrument just can detect, as Biochemical Analyzer etc.; Enzyme process needs to use multiple enzyme to coordinate to carry out reacting and just can obtain net result, and net result also needs large-scale instrument to detect.
After various method all needs to detect total hemoglobin and glycosylated hemoglobin two indices respectively above, the number percent accounting for total hemoglobin by calculating glycosylated hemoglobin just can obtain net result, the direct result that twice detection brings is exactly that testing result accuracy is not high, and prior art complicated operation, professional person must be had to operate and just can carry out.In addition, the instrument price of prior art use is expensive and bulky.
Therefore, be necessary research and development a kind of highly sensitive, specificity good and glycosylated hemoglobin detection kit easy and simple to handle.
Summary of the invention
In order to overcome the shortcoming and defect existed in prior art, the purpose of this utility model be to provide a kind of highly sensitive, specificity good and glycosylated hemoglobin detection kit easy and simple to handle.
The purpose of this utility model is achieved through the following technical solutions: a kind of glycosylated hemoglobin detection kit, described kit comprises box body, be layed in the liner of bottom inside and be lining with in being positioned over aluminium foil bag, reagent bottle, chromatographic column and capillary heparin tube, brown centrifuge tube is equiped with in aluminium foil bag, brown centrifuge tube is built with the first reagent, reagent bottle is built with the second reagent, and chromatographic column comprises shell and is fixed on the reaction film in shell.
Further, the capacity of described brown centrifuge tube is 1mL, and the cumulative volume of described first reagent is 200 μ L.
Further, the capacity of described reagent bottle is 5mL, and the cumulative volume of described second reagent is 2mL.
Further, the aperture of described reaction film is 0.5 μm.
Further, described reaction film is cation-exchange membrane.
Further, described shell comprises feed pipe and drain pipe, and the bottom of feed pipe is fixed with annular lug, and the top of drain pipe offers the annular groove matched with annular lug, annular lug inserts in annular groove, and reaction film is located between the bottom surface of annular lug and the bottom surface of annular groove.
Further, the bottom of described annular groove offers conical cavity, and the bottom of conical cavity offers liquid outlet.
Further, the lateral wall of described annular lug is provided with external thread, and the madial wall of described annular groove is provided with internal thread, and annular lug and annular groove are spirally connected.
Further, described shell is polytetrafluoroethylplastic plastic shell.
The beneficial effects of the utility model are: kit of the present utility model, by employing first reagent, the second reagent and reaction film, detects glycosylated hemoglobin.
Kit of the present utility model only needs the whole blood of trace to live peripheral blood sample, can realize the content quantitatively detecting glycosylated hemoglobin, greatly improve the speed of examination in 2-3 minute, have highly sensitive, specificity good and advantage easy and simple to handle.
Accompanying drawing explanation
Fig. 1 is structural representation of the present utility model.
Fig. 2 is the cut-open view of chromatographic column described in the utility model.
Reference numeral is: 1-box body, 2-liner, 3-aluminium foil bag, 31-brown centrifuge tube, 4-reagent bottle, 5-chromatographic column, 50-reaction film, 51-feed pipe, 511-annular lug, 52-drain pipe, 521-annular groove, 522-conical cavity, 523-liquid outlet, 6-capillary heparin tube.
Embodiment
For the ease of the understanding of those skilled in the art, be further described the utility model below in conjunction with embodiment and accompanying drawing 1-2, the content that embodiment is mentioned is not to restriction of the present utility model.
See Fig. 1-2, a kind of glycosylated hemoglobin detection kit, described kit comprises box body 1, is layed in the liner 2 of box body 1 bottom inside wall, be arranged at the aluminium foil bag 3 at liner 2 top, reagent bottle 4, chromatographic column 5 and capillary heparin tube 6, brown centrifuge tube 31 is equiped with in aluminium foil bag 3, brown centrifuge tube 31 is built with the first reagent, and reagent bottle 4 is built with the second reagent, and chromatographic column 5 comprises shell and is fixed on the reaction film 50 in shell.
Described first reagent comprises the borate derivative for splitting erythrocyte, release glycosylated hemoglobin, precipitation total hemoglobin.
Preferably, often liter of described first reagent comprises following component:
Borate derivative 0.4-0.6g
Magnesium chloride 1.9-2.9g
Potassium chloride 22-26g
Barium chloride 6.2-8.2g
Glycyl amide hydrochloride 6.6-8.6g
Formamide 25-35g
Potassium azide 8-12g
NPE 15-25g
Water surplus.
Preferably, the structural formula of described borate derivative is:
，
Wherein, R
1for azine, R
2for carbon number is the alkyl of 1-6.
Described second reagent comprises the damping fluid for washing away the borate derivative in conjunction with glycosylated hemoglobin.
Preferably, often liter of described second reagent comprises following component:
4-hydroxyethyl piperazine ethanesulfonic acid 23-33g
Potassium azide 4-6g
Sodium chloride 0.4-0.8g
Formamide 8-12g
Triton X-100 4-6g
Water surplus.
In the present embodiment, the capacity of described brown centrifuge tube 31 is 1mL, and the cumulative volume of described first reagent is 200 μ L.First reagent is borate derivative, pH8.0, and total amount 200 μ L, is placed in the brown centrifuge tube 31 of 1mL capacity.
In the present embodiment, the capacity of described reagent bottle 4 is 5mL, and the cumulative volume of described second reagent is 2mL.Second reagent is damping fluid, pH8.0, total amount 2mL, is placed in 5mL capacity reagent bottle 4.
In the present embodiment, the aperture of described reaction film is 0.5 μm.Said structure be provided with the borate derivative being beneficial to and washing away in conjunction with glycosylated hemoglobin, and retain haemoglobin precipitation.
Kit of the present utility model passes through employing first reagent, for the borate derivative of splitting erythrocyte, release glycosylated hemoglobin, precipitation total hemoglobin; By adopting the second reagent, for washing away the borate derivative in conjunction with glycosylated hemoglobin; Again by adopting the reaction film 50 for retaining haemoglobin precipitation, glycosylated hemoglobin is detected.
Kit of the present utility model only needs the whole blood of trace to live peripheral blood sample, can realize the content quantitatively detecting glycosylated hemoglobin, greatly improve the speed of examination in 2-3 minute, have highly sensitive, specificity good and advantage easy and simple to handle.
Described shell comprises feed pipe 51 and drain pipe 52, the bottom of feed pipe 51 is fixed with annular lug 511, the top of drain pipe 52 offers the annular groove 521 matched with annular lug 511, annular lug 511 inserts in annular groove 521, and reaction film 50 is located between the bottom surface of annular lug 511 and the bottom surface of annular groove 521.
Chromatographic column 5 of the present utility model can be separated glycosylated hemoglobin under the condition of normal pressure; The impact of glycosylated hemoglobin interference component can be reduced; Ensure the reliability of the measurement result of glycosylated hemoglobin, structure is simple, and volume is little, and cost is low.
Described reaction film 50 is cation-exchange membrane.Cation-exchange membrane be provided with the borate derivative being beneficial to and washing away in conjunction with glycosylated hemoglobin, and retain haemoglobin precipitation.
The bottom of described annular groove 521 offers conical cavity 522, and the bottom of conical cavity 522 offers liquid outlet 523.Arranging of conical cavity 522 can accelerate Tomography Velocity.
The lateral wall of described annular lug 511 is provided with external thread, and the madial wall of described annular groove 521 is provided with internal thread, and annular lug 511 and annular groove 521 are spirally connected.The dismounting of feed pipe 51 and drain pipe 52 is convenient in the setting be spirally connected.
Described shell is polytetrafluoroethylplastic plastic shell.Polytetrafluoroethylplastic plastic shell has the feature of antiacid alkali resistant, anti-various organic solvent, is dissolved in all solvents hardly.
Above-described embodiment is the utility model preferably implementation, and in addition, the utility model can also realize by alternate manner, and any apparent replacement is all within protection domain of the present utility model without departing from the concept of the premise utility.
Claims (5)
1. a glycosylated hemoglobin detection kit, it is characterized in that: described kit comprises box body, the aluminium foil bag, reagent bottle, chromatographic column and the capillary heparin tube that are layed in the liner of bottom inside and are lining with in being positioned over, brown centrifuge tube is equiped with in aluminium foil bag, brown centrifuge tube is built with the first reagent, reagent bottle is built with the second reagent, and chromatographic column comprises shell and is fixed on the reaction film in shell.
2. a kind of glycosylated hemoglobin detection kit according to claim 1, is characterized in that: the capacity of described brown centrifuge tube is 1mL, and the cumulative volume of described first reagent is 200 μ L.
3. a kind of glycosylated hemoglobin detection kit according to claim 1, is characterized in that: the capacity of described reagent bottle is 5mL, and the cumulative volume of described second reagent is 2mL.
4. a kind of glycosylated hemoglobin detection kit according to claim 1, is characterized in that: the aperture of described reaction film is 0.5 μm.
5. a kind of glycosylated hemoglobin detection kit according to claim 1, is characterized in that: described reaction film is cation-exchange membrane.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520392455.2U CN204789592U (en) | 2015-06-09 | 2015-06-09 | Glycated haemoglobin detect reagent box |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520392455.2U CN204789592U (en) | 2015-06-09 | 2015-06-09 | Glycated haemoglobin detect reagent box |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204789592U true CN204789592U (en) | 2015-11-18 |
Family
ID=54529609
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520392455.2U Expired - Fee Related CN204789592U (en) | 2015-06-09 | 2015-06-09 | Glycated haemoglobin detect reagent box |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN204789592U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109212237A (en) * | 2018-08-31 | 2019-01-15 | 深圳市国赛生物技术有限公司 | Kit components are used in glycosylated hemoglobin detection |
| CN109212233A (en) * | 2018-08-31 | 2019-01-15 | 深圳市国赛生物技术有限公司 | Kit components are used in glycosylated hemoglobin detection |
-
2015
- 2015-06-09 CN CN201520392455.2U patent/CN204789592U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109212237A (en) * | 2018-08-31 | 2019-01-15 | 深圳市国赛生物技术有限公司 | Kit components are used in glycosylated hemoglobin detection |
| CN109212233A (en) * | 2018-08-31 | 2019-01-15 | 深圳市国赛生物技术有限公司 | Kit components are used in glycosylated hemoglobin detection |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20151118 Termination date: 20170609 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |