CN109212237A - Kit components are used in glycosylated hemoglobin detection - Google Patents
Kit components are used in glycosylated hemoglobin detection Download PDFInfo
- Publication number
- CN109212237A CN109212237A CN201811025365.4A CN201811025365A CN109212237A CN 109212237 A CN109212237 A CN 109212237A CN 201811025365 A CN201811025365 A CN 201811025365A CN 109212237 A CN109212237 A CN 109212237A
- Authority
- CN
- China
- Prior art keywords
- chamber
- pipette tips
- reagent
- glycosylated hemoglobin
- accommodating chamber
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
- G01N33/721—Haemoglobin
- G01N33/723—Glycosylated haemoglobin
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Hematology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The present invention discloses a kind of glycosylated hemoglobin detection kit components, wherein, glycosylated hemoglobin detection kit components include kit, sampler, the first pipette tips and the second pipette tips, the first mutually isolated reagent chamber, the second reagent chamber, analysis cavity and pipette tips accommodating chamber are offered on kit, first reagent chamber is for holding reaction solution, and reaction compartment is provided for reaction solution and blood sample, the second reagent is for holding eluent；Sampler is for acquiring blood sample and blood sample being injected to the first reagent chamber；First pipette tips and the second pipette tips are contained in pipette tips accommodating chamber, and the first pipette tips are for drawing the reaction mixture in the first reagent chamber and transferring them in analysis cavity, and the second pipette tips are for drawing the eluent in the second reagent chamber and transferring them in analysis cavity.Glycosylated hemoglobin detection of the present invention draws reaction solution and eluent using different pipette tips with kit components respectively, and reaction precision is improved while avoiding cross contamination.
Description
Technical field
The present invention relates to glycosylated hemoglobin detection field, in particular to a kind of glycosylated hemoglobin detection kit group
Part.
Background technique
Glycohemoglobin HbA1c is product of the hemoglobin in conjunction with blood glucose in red blood cell in blood.According to international sugar
Map the 8th edition report of global diabetes of 2017 of Niao Bing alliance (IDF) newest publication, there are 4.25 hundred million adults in the whole world within 2017
It is diabetic, year-on-year upper one year increases by 2.41%.There are about 1.144 hundred million diabetics for China, separately there is ten thousand glycosuria more than 8750
" reserves " of patient's high risk.The diabetic for being estimated to be half does not obtain comprehensive diagnosis management.Diabetes
Treatment key be blood glucose level control and stabilization, at present to the diagnosis of diabetes mainly pass through detection patient on an empty stomach and after meal
2 hours blood glucose values are judged, but influence vulnerable to the diet and held drug etc. of patient in the sampling observation period, no
The truth of patient's body blood glucose can be objectively responded.Studies have shown that the average life span of hemoglobin is up to 120 days, and HbA1c
Formation speed to glucose concentration level is directly proportional in 8-12 weeks in the past, therefore, HbA1c level can more accurately reflect the past
The intracorporal blood glucose situation of 8-12 weeks people.And glycosylated hemoglobin not by the blood drawing time, whether on an empty stomach, whether using insulin and
Other can be such that the factor of blood glucose level brief fluctuations influences.Glycosylated hemoglobin detects high specificity, reproducible, sensitivity
Height is expressly stated and monitors " goldstandard " for internationally recognized diabetes.
Existing by rotating kit reagent therein to be reacted with blood sample, such mode can not essence
The really amount of control reaction reagent, also has through sampler or draws different reagents using the same pipette tips and reacted, and
This mode is easy to happen cross contamination, so that test data deviation is larger.
Summary of the invention
The main object of the present invention is to propose a kind of glycosylated hemoglobin detection kit components, it is intended to avoid intersecting dirty
The technical issues of reaction precision is improved while dye.
To achieve the above object, glycosylated hemoglobin proposed by the present invention detection kit components, including kit, adopt
Sample device, the first pipette tips and the second pipette tips；The first mutually isolated reagent chamber is offered on the kit, the second reagent chamber, is divided
Chamber and pipette tips accommodating chamber are analysed, first reagent chamber reacts to obtain instead with blood sample for holding reaction solution, and for reaction solution
Mixture is answered to provide reaction compartment, second reagent chamber is for holding eluent；The sampler is for acquiring blood sample
And the blood sample is injected to first reagent chamber；First pipette tips and the second pipette tips are contained in the pipette tips receiving
Intracavitary, first pipette tips are for drawing the reaction mixture in first reagent chamber and transferring them to the analysis cavity
Interior, second pipette tips are for drawing the eluent in second reagent chamber and transferring them in the analysis cavity.
Preferably, first reagent chamber and second reagent chamber are disposed proximate to；The pipette tips accommodating chamber includes being used for
Place the first accommodating chamber of first pipette tips and the second accommodating chamber for placing second pipette tips, first accommodating chamber
Neighbouring first reagent chamber is arranged, and second accommodating chamber is arranged adjacent to second reagent chamber.
Preferably, it is additionally provided on the kit and moves back pipette tips chamber, the pipette tips chamber that moves back is for accommodating described the after use
One pipette tips and second pipette tips.
Preferably, the pipette tips chamber that moves back is arranged adjacent to the pipette tips accommodating chamber, and the end of the open-position-side for moving back pipette tips chamber
Face and the end face of the pipette tips accommodating chamber open-position-side are arranged in step.
Preferably, the pipette tips accommodating chamber close to the side for moving back pipette tips chamber in semi arch openning shape so that described the
First pipette tips described in when one pipette tips and second pipette tips are placed in the pipette tips accommodating chamber and second pipette tips part are exposed.
Preferably, the pipette tips chamber that moves back includes the first chamber and the second chamber, and first chamber is adjacent to first accommodating chamber
Side far from first reagent chamber is arranged；Second chamber is adjacent to second accommodating chamber far from first reagent chamber
Side setting.
Preferably, the kit components further include sealing film, and the sealing film corresponds to first reagent chamber and described
The open setting of second reagent chamber, and the top of the pipette tips accommodating chamber is extended to pipette tips accommodating chamber described in partial occlusion.
Preferably, it is additionally provided on the kit and moves back sampler chamber for accommodate the sampler after use, described move back is adopted
The neighbouring side far from the pipette tips accommodating chamber for moving back pipette tips chamber of sample device chamber is arranged.
Preferably, first reagent chamber, second reagent chamber, the pipette tips accommodating chamber, the sampler chamber that moves back
Axis is parallel to each other and is in the same plane.
Preferably, filter membrane is equipped in the analysis room, the analysis cavity is divided on the filter membrane by the filter membrane
The analysis room of side and the waste liquid chamber on the downside of the filter membrane, the analysis room are reacted for placing response liquid and blood sample
Mixture, the waste liquid chamber are used to accept the eluent after cleaning the reaction mixture.
Glycosylated hemoglobin detection kit components of the present invention are drawn in the first reagent chamber by using the first pipette tips
Reaction mixture simultaneously transfers them in analysis cavity, draws the eluent in the second reagent chamber using the second pipette tips and is shifted
To analysis cavity, the amount for drawing liquid so can not only be accurately controlled, so that reaction is more accurate, while can be avoided pipette tips
Cross contamination occurs and experimental data is caused to generate relatively large deviation.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technical description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this
Some embodiments of invention for those of ordinary skill in the art without creative efforts, can be with
The structure shown according to these attached drawings obtains other attached drawings.
Fig. 1 is the structural schematic diagram that one embodiment of kit components is used in glycosylated hemoglobin of the present invention detection；
Fig. 2 is that glycosylated hemoglobin detection kit components are inserted into the schematic diagram of the section structure after sampler in Fig. 1；
Fig. 3 is the structural schematic diagram that another embodiment of kit components is used in glycosylated hemoglobin of the present invention detection；
Fig. 4 is that glycosylated hemoglobin detection kit components are inserted into the schematic diagram of the section structure after sampler in Fig. 3；
Fig. 5 is the structural schematic diagram that kit components another embodiment is used in glycosylated hemoglobin of the present invention detection；
Fig. 6 is that glycosylated hemoglobin detection kit components are inserted into the schematic diagram of the section structure after sampler in Fig. 5.
Drawing reference numeral explanation:
The embodiments will be further described with reference to the accompanying drawings for the realization, the function and the advantages of the object of the present invention.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiment is only a part of the embodiments of the present invention, instead of all the embodiments.Base
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts it is all its
His embodiment, shall fall within the protection scope of the present invention.
It is to be appreciated that if relating to directionality instruction (such as up, down, left, right, before and after ...) in the embodiment of the present invention,
Then directionality instruction be only used for explain under a certain particular pose (as shown in the picture) between each component relative positional relationship,
Motion conditions etc., if the particular pose changes, directionality instruction is also correspondingly changed correspondingly.
In addition, being somebody's turn to do " first ", " second " etc. if relating to the description of " first ", " second " etc. in the embodiment of the present invention
Description be used for description purposes only, be not understood to indicate or imply its relative importance or implicitly indicate indicated skill
The quantity of art feature." first " is defined as a result, the feature of " second " can explicitly or implicitly include at least one spy
Sign.In addition, the meaning of the "and/or" occurred in full text is, and including three schemes arranged side by side, by taking " A and/or B " as an example, including A
The scheme that scheme or B scheme or A and B meet simultaneously.In addition, the technical solution between each embodiment can be combined with each other,
It but must be based on can be realized by those of ordinary skill in the art, when conflicting or nothing occurs in the combination of technical solution
Method realize when will be understood that the combination of this technical solution is not present, also not the present invention claims protection scope within.
The present invention proposes a kind of glycosylated hemoglobin detection kit components.
In embodiments of the present invention, as shown in Figures 1 and 2, glycosylated hemoglobin detection kit components, including examination
Agent box 1, sampler 2, the first pipette tips 3 and the second pipette tips 4；The first mutually isolated reagent chamber 11, second is offered on kit 1
Reagent chamber 12, analysis cavity 13 and pipette tips accommodating chamber 14, the first reagent chamber 11 are reaction solution and blood sample for holding reaction solution
Product react to obtain reaction mixture offer reaction compartment, and the second reagent chamber 12 is for holding eluent；Sampler 2 is for acquiring blood
Blood sample is simultaneously injected to the first reagent chamber 11 by liquid sample；First pipette tips 3 and the second pipette tips 4 are contained in pipette tips accommodating chamber 14
Interior, the first pipette tips 3 are for drawing the reaction mixture in the first reagent chamber 11 and transferring them in analysis cavity 13, the second pipette tips
4 for drawing the eluent in the second reagent chamber 12 and transferring them in analysis cavity 13.
In the present embodiment, without limitation, cross sectional shape can be in rectangle or circle etc. for the shape of kit 1.Instead
It answers liquid for being reacted with blood sample, obtains the amount of glycosylated hemoglobin in blood sample mixture.For example, reaction solution contains
The blue for having cleavable red blood cell and making the substance of hemoglobin precipitating and being closed with the syn-diol base junction of glycosylated hemoglobin
Boric acid conjugate.Eluent obtains final required test substances for washing away dyeing conjugate extra in reaction mixture.
When blood sample instills in reaction solution, red blood cell is cleaved immediately, all hemoglobin precipitatings, boric acid conjugate then with saccharification blood
The syn-diol base junction of Lactoferrin is closed.Suitable reaction mixture is placed in reaction chamber, all settled hemoglobins without
Whether combined by boric acid conjugate by it, will all remain in reaction chamber, then washes away extra dyeing with eluent and be conjugated
Object, waste liquid flow into waste liquid chamber.It is finally (total blood red with red to blue (glycosylated hemoglobin) using glycosylated hemoglobin detector
Albumen) respectively wavelength 625nm and 460nm light irradiation under reflectivity analyzed, obtain the concentration of HbA1c in blood sample.
The micropipet of sampler 2 directly acquires blood sample (such as finger blood) with human contact, and the sampler 2 is when unused
Using independent packaging, and production meets the requirement of third mankind's medical instrument, meets the requirement of sterilization.First pipette tips
3 and second pipette tips 4 draw liquid accuracy it is higher than the accuracy of the absorption liquid of sampler 2, and can accurately control
Draw the amount of liquid.Pipette tips accommodating chamber 14 can be used for accommodating the pipette tips after pipette tips and use before use, pipette tips accommodating chamber 14
Quantity can be one or more, when the quantity of pipette tips accommodating chamber 14 be one when, can be by the first pipette tips 3 and the second rifle
First 4 are nested together, so saving space, so that the volume of 1 entirety of kit is smaller.
It is understood that the first reagent chamber 11, the second reagent chamber 12, reaction chamber, pipette tips accommodating chamber 14, the first pipette tips 3
And second pipette tips 4 can be set to cover, in this way, test of many times can be carried out on a kit 1 or to multiple use
Family is tested, and experimentation cost is saved, so that test is more convenient.During the experiment, blood sample need and reaction solution into
Row reaction, so that the first reagent chamber 11 provides reaction compartment for blood sample, then the reaction of the two can be in the first reagent chamber 11
Middle progress is reacted with reaction solution for blood sample in this way, a reaction chamber need not in addition be arranged, and need to be only placed in blood sample
It is reacted in reaction solution, reduces testing procedure, while simplifying the structure of kit 1.
Glycosylated hemoglobin detection kit components of the present invention are drawn in the first reagent chamber 11 by using the first pipette tips 3
Reaction mixture and transfer them in analysis cavity 13, using the second pipette tips 4 draw the second reagent chamber 12 in eluent simultaneously
It transfers them in analysis cavity 13, the amount for drawing liquid so can not only be accurately controlled, so that reaction is more accurate, while energy
It enough avoids pipette tips that cross contamination occurs and experimental data is caused to generate relatively large deviation.
Further, the first reagent chamber 11 and the second reagent chamber 12 are disposed proximate to；Pipette tips accommodating chamber 14 includes for placing
First accommodating chamber 141 of the first pipette tips 3 and the second accommodating chamber 142 for placing the second pipette tips 4, the first accommodating chamber 141 are neighbouring
The setting of first reagent chamber 11, the second accommodating chamber 142 are arranged adjacent to the second reagent chamber 12.
In the present embodiment, the first accommodating chamber 141 accommodates the first pipette tips 3,4 chamber of the second pipette tips accommodates the second pipette tips 4, then makes
It obtains the first pipette tips 3 and the second pipette tips 4 is separated, without being nested together, pick and place the first pipette tips 3 and second to easily facilitate
Pipette tips 4.First accommodating chamber 141 is arranged adjacent to the first reagent chamber 11, so that the first pipette tips 3 are extracted from the first accommodating chamber 141
The path for being moved to absorption reaction solution in the first reagent chamber 11 is shorter, and similarly, the second accommodating chamber 142 is set adjacent to the second reagent chamber 12
It sets, draws the path of eluent more so that the second pipette tips 4 are moved in the second reagent chamber 12 from extraction in the second accommodating chamber 142
It is short, to save experimental period.And user so can effectively be avoided to obscure the first pipette tips 3 and the second pipette tips 4, reduction pair
Human error caused by experimental result.
Further, it please with reference to Fig. 3 and Fig. 4, is additionally provided on kit 1 and moves back pipette tips chamber 15, moved back pipette tips chamber 15 and be used for
The first pipette tips 3 and the second pipette tips 4 after accommodating use.
In the present embodiment, it is to be understood that glycosylated hemoglobin detection kit components can be with HbAle
Protein analyzer is used cooperatively, and pipette tips are backed off by being arranged to move back pipette tips chamber 15 and easily facilitate the chamber device that moves back of analyzer.And it moves back
Pipette tips chamber 15 accommodates the first pipette tips 3 and the second pipette tips 4 after use so that not used first pipette tips 3 and the second pipette tips 4 with make
The first pipette tips 3 and the second pipette tips 4 after are separated, and the pipette tips after is avoided to pollute not used pipette tips.Move back pipette tips chamber
15 quantity can be one or more, can be by the first pipette tips 3 and second when the quantity for moving back pipette tips accommodating chamber 14 is one
Pipette tips 4 are nested together, so saving space, so that the volume of 1 entirety of kit is smaller.
Specifically, it moves back pipette tips chamber 15 to be arranged adjacent to pipette tips accommodating chamber 14, and moves back end face and the rifle of the open-position-side of pipette tips chamber 15
The end face of head 14 open-position-side of accommodating chamber is arranged in step.In this way, moving back end face and the pipette tips accommodating chamber 14 of 15 open-position-side of pipette tips chamber
Difference in height is formed between the end face of open-position-side, then it, can be convenient on analyzer when 1 component of kit and analyzer are used cooperatively
The operation for moving back pipette tips mechanism, i.e., convenient for pipette tips are retreated in pipette tips chamber 15.Preferably, the end of the open-position-side of pipette tips chamber 15 is moved back
End face of the face lower than the open-position-side of pipette tips accommodating chamber 14.
Further, pipette tips accommodating chamber 14 is in semi arch openning shape close to the side for moving back pipette tips chamber 15, so that the first pipette tips 3
And second pipette tips 4 when being placed in pipette tips accommodating chamber 14 first pipette tips 3 and 4 part of the second pipette tips it is exposed.It so, it is possible so that reagent
The structure of box 1 is more compact, so that the volume of kit 1 is smaller, more saves space.
Further, please with reference to Fig. 5 and Fig. 6, moving back pipette tips chamber 15 includes the first chamber 151 and the second chamber 152, the first chamber
151 are arranged adjacent to the side far from the first reagent chamber 11 of the first accommodating chamber 141；Second chamber 152 is adjacent to the second accommodating chamber 142
Side far from the first reagent chamber 11 is arranged.
In this embodiment, by the first chamber 151 of setting and the second chamber 152, so that the first pipette tips 3 backed off and the second pipette tips
4 do not have to be nested together, so convenient for the first pipette tips 3 and the second pipette tips 4 after placement use.Preferably, the first chamber 151 is spacious
End face at mouthful is concordant with the end face of open-mouth of the second chamber 152, in this way, making when being used cooperatively kit 1 and analyzer
Chamber device is moved back just and can unloaded the first pipette tips 3 and the second pipette tips 4 with same, simplifies the structure of whole device.
Further, 1 component of kit further includes sealing film 5, corresponding first reagent chamber 11 of sealing film 5 and the second reagent chamber
12 open setting, and the top of pipette tips accommodating chamber 14 is extended to partial occlusion pipette tips accommodating chamber 14.
In the present embodiment, sealing film 5 is used to seal the opening of the first reagent chamber 11 and the second reagent chamber 12.It is understood that
, before factory, reaction solution and eluent are first pre-loaded into the first reagent chamber 11 and the second reagent chamber 12, then using sealing
Film 5 is sealed, and prevents experiment reagent from outflowing or being contaminated, in this way, when leaving the factory, being encapsulated in kit 1 by sealing film 5
Reaction solution and eluent dispatched from the factory together with kit 1 so that user is more convenient to use.During the experiment, it needs to make
When with experiment reagent, sampler 2 or pipette tips are directly pierced through into sealing film 5, protruded into required reagent chamber.Sealing film 5 is prolonged
The top of pipette tips accommodating chamber 14 is extended to partial occlusion pipette tips accommodating chamber 14, after pipette tips are mounted in pipette tips accommodating chamber 14 in advance, due to
Sealing film 5 partly blocks, so that the first pipette tips 3 and the second pipette tips 4 can not be dropped out from kit 1.Preferably, the first reagent chamber
The opening of 11 opening and the second reagent chamber 12 is generally aligned in the same plane, and sealing film 5 covers the first reagent chamber 11 and the second examination simultaneously
The opening of agent chamber 12.
In a preferred embodiment, tearing is set at the insertion position for corresponding to sampler 2 on the outer surface of sealing film 5
Line.Tear line is set in the position of corresponding first reagent chamber 11 of sealing film 5.Such sampler 2 can accurately be inserted into first
It in reagent chamber 11 and is put into kit 1, to be manually properly inserted sampler 2 by tear line after sampling, avoids adopting
Sample device 2 is made mistakes in insertion, is caused the loss of sample, is significantly improved effective sample rate.
Further, it is additionally provided on kit 1 and moves back 2 chamber of sampler for accommodate the sampler 2 after use, move back sampler
The side setting of the neighbouring separate pipette tips accommodating chamber 14 for moving back pipette tips chamber 15 of 2 chambers.
In the present embodiment, 2 chamber of sampler is moved back by being arranged on kit 1, so that the sampler 2 after use is in reagent
There is accommodation space on box 1, the structure for accommodating sampler 2 so need not be additionally set, saved cost, and use is more square
Just.
Further, the first reagent chamber 11, the second reagent chamber 12, pipette tips accommodating chamber 14, move back 2 chamber of sampler axis it is mutual
In parallel and it is in the same plane.In this way, analyzer is mobile when kit 1 and glycolated hemoglobin analysis are used cooperatively
Only need linear motion in one direction that can respectively correspond the sample adding device of analyzer on kit 1 when kit 1
Different cavity position so that the structure of analyzer is simpler, volume is smaller, operate it is more convenient.First when use
Pipette tips 3 and the second pipette tips 4 are first mounted in pipette tips accommodating chamber 14 in advance, in this way, only needing manually to place the sampler 2 for being collected blood sample
In the first reagent chamber 11, entire glycosylated hemoglobin detection is put into analyzer with kit components then, passes through reagent
The sample adding device cooperation for each cavity and analyzer that box 1 is arranged can realize that full automatic mixing detects, high degree of automation,
Artificial participation step is few.
In a preferred embodiment, filter membrane 17 is equipped in analysis room 131, analysis cavity 13 is divided into positioned at filter membrane by filter membrane 17
The analysis room 131 of 17 upsides and the waste liquid chamber 132 on the downside of filter membrane 17, analysis room 131 is for placing response liquid and blood sample
The reaction mixture of product, waste liquid chamber 132 are used to accept the eluent after cleaning reaction mixture.
In the present embodiment, support knot can be arranged in filter membrane 17 by insertion 13 inner wall of analysis cavity or in analysis cavity 13
Filter membrane 17 is simultaneously placed on the mode on supporting mechanism and is located in analysis cavity 13 by structure, and analysis cavity 13 is divided into analysis room 131
With waste liquid chamber 132.Analysis cavity 13 is divided into analysis room 131 and waste liquid chamber 132 using filter membrane 17, obtained final detection substance
It staying on filter membrane 17, extra dyeing conjugate and eluent flow into waste liquid chamber 132, and avoid detection substance from mixing with waste liquid, from
And improve the measuring accuracy of glycosylated hemoglobin.
Preferably, the internal diameter of waste liquid chamber 132 is less than the internal diameter of analysis room 131, so that the inner wall and waste liquid of analysis room 131
The junction of the inner wall of room 132 forms step surface, and filter membrane 17 is placed on step surface.Kit 1 further includes for being pressed on
The filter membrane lid of 17 upper surface of filter membrane, filter membrane cover the flow-guiding channel for being equipped with perforation filter membrane lid setting；Flow-guiding channel has in flaring
The flow-guiding mouth of shape setting.
In the present embodiment, filter membrane 17 is placed on step surface, and filter membrane lid is pressed on the upper surface of filter membrane lid, by filter membrane 17
It is pressed on step surface, so that filter membrane 17 is less likely to occur to crimp and shift.Flow-guiding channel is for by eluent or instead
Mixture is answered to be directed on filter membrane 17.On the one hand flow-guiding mouth concentrates on more light on filter membrane 17 in flared, thus
So that detection effect is more preferable, it is on the other hand in that the flow-guiding mouth of flared can play buffering and water conservancy diversion to the fluid flowed into it
Effect, so as to increase the fluid flow in injection analysis cavity 13 and avoid liquid spluttering.Filter membrane lid can directly pass through
The mode of interference fit is embedded in analysis cavity 13, convex shoulder can also be arranged in one end of the separate filter membrane 17 of filter membrane lid, so that
When filter membrane lid is pressed against filter membrane 17, convex shoulder abuts the end face of reaction chamber, so that filter membrane lid is assembled in reaction chamber.
The above description is only a preferred embodiment of the present invention, is not intended to limit the scope of the invention, all at this
Under the inventive concept of invention, using equivalent structure transformation made by description of the invention and accompanying drawing content, or directly/use indirectly
It is included in other related technical areas in scope of patent protection of the invention.
Claims (10)
1. a kind of glycosylated hemoglobin detection kit components characterized by comprising
Kit offers the first mutually isolated reagent chamber, the second reagent chamber, analysis cavity and pipette tips receiving on the kit
Chamber, first reagent chamber are reacted with blood sample for reaction solution to obtain reaction mixture and provided instead for holding reaction solution
Space is answered, second reagent chamber is for holding eluent；
Sampler, for acquiring blood sample and the blood sample being injected to first reagent chamber；And
First pipette tips and the second pipette tips are contained in the pipette tips accommodating chamber, and first pipette tips are for drawing first examination
The intracavitary reaction mixture of agent simultaneously transfers them in the analysis cavity, and second pipette tips are for drawing second reagent chamber
Interior eluent simultaneously transfers them in the analysis cavity.
2. glycosylated hemoglobin detection kit components as described in claim 1, which is characterized in that first reagent chamber
It is disposed proximate to second reagent chamber；The pipette tips accommodating chamber include for place the first accommodating chamber of first pipette tips and
For placing the second accommodating chamber of second pipette tips, first accommodating chamber is arranged adjacent to first reagent chamber, and described
Two accommodating chambers are arranged adjacent to second reagent chamber.
3. glycosylated hemoglobin detection kit components as claimed in claim 2, which is characterized in that on the kit also
Equipped with pipette tips chamber is moved back, the pipette tips chamber that moves back is for accommodating first pipette tips and second pipette tips after use.
4. glycosylated hemoglobin detection kit components as claimed in claim 3, which is characterized in that described to move back pipette tips chamber neighbour
The nearly pipette tips accommodating chamber setting, and the end face of the end face of the open-position-side for moving back pipette tips chamber and the pipette tips accommodating chamber open-position-side
It is arranged in step.
5. glycosylated hemoglobin detection kit components as claimed in claim 4, which is characterized in that the pipette tips accommodating chamber
Close to the side for moving back pipette tips chamber in semi arch openning shape so that first pipette tips and second pipette tips be placed in it is described
First pipette tips and second pipette tips part described in when pipette tips accommodating chamber are exposed.
6. glycosylated hemoglobin detection kit components as claimed in claim 3, which is characterized in that described to move back pipette tips chamber packet
The first chamber and the second chamber are included, first chamber is arranged adjacent to the side far from first reagent chamber of first accommodating chamber；
Second chamber is arranged adjacent to the side far from first reagent chamber of second accommodating chamber.
7. glycosylated hemoglobin detection kit components as described in claim 1, which is characterized in that the kit components
It further include sealing film, the sealing film corresponds to the open setting of first reagent chamber and second reagent chamber, and extends to
The top of the pipette tips accommodating chamber is with pipette tips accommodating chamber described in partial occlusion.
8. glycosylated hemoglobin detection kit components as claimed in claim 3, which is characterized in that on the kit also
It is described to move back the neighbouring separate institute for moving back pipette tips chamber of sampler chamber equipped with moving back sampler chamber for accommodate the sampler after use
State the side setting of pipette tips accommodating chamber.
9. glycosylated hemoglobin detection kit components as claimed in claim 8, which is characterized in that first reagent
Chamber, second reagent chamber, the pipette tips accommodating chamber, the axis for moving back sampler chamber are parallel to each other and are generally aligned in the same plane
It is interior.
10. glycosylated hemoglobin detection kit components as in one of claimed in any of claims 1 to 9, which is characterized in that
Filter membrane is equipped in the analysis room, the analysis cavity is divided into the analysis room on the upside of the filter membrane and is located at by the filter membrane
Waste liquid chamber on the downside of the filter membrane, the analysis room are used for the reaction mixture of placing response liquid and blood sample, the waste liquid
Room is used to accept the eluent after cleaning the reaction mixture.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811025365.4A CN109212237A (en) | 2018-08-31 | 2018-08-31 | Kit components are used in glycosylated hemoglobin detection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811025365.4A CN109212237A (en) | 2018-08-31 | 2018-08-31 | Kit components are used in glycosylated hemoglobin detection |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109212237A true CN109212237A (en) | 2019-01-15 |
Family
ID=64986043
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811025365.4A Pending CN109212237A (en) | 2018-08-31 | 2018-08-31 | Kit components are used in glycosylated hemoglobin detection |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109212237A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202204820U (en) * | 2011-08-12 | 2012-04-25 | 石家庄禾柏生物技术股份有限公司 | Integrated combined kit |
| CN204789592U (en) * | 2015-06-09 | 2015-11-18 | 广东优尼德生物科技有限公司 | Glycated haemoglobin detect reagent box |
| US20160107161A1 (en) * | 2011-09-30 | 2016-04-21 | Becton, Dickinson And Company | Unitized reagent strip |
| CN205484377U (en) * | 2016-03-24 | 2016-08-17 | 三诺生物传感股份有限公司 | Detection kit |
| CN107219350A (en) * | 2017-05-31 | 2017-09-29 | 深圳市国赛生物技术有限公司 | A kind of kit |
-
2018
- 2018-08-31 CN CN201811025365.4A patent/CN109212237A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202204820U (en) * | 2011-08-12 | 2012-04-25 | 石家庄禾柏生物技术股份有限公司 | Integrated combined kit |
| US20160107161A1 (en) * | 2011-09-30 | 2016-04-21 | Becton, Dickinson And Company | Unitized reagent strip |
| EP3273253A1 (en) * | 2011-09-30 | 2018-01-24 | Becton, Dickinson and Company | Unitized reagent strip |
| CN204789592U (en) * | 2015-06-09 | 2015-11-18 | 广东优尼德生物科技有限公司 | Glycated haemoglobin detect reagent box |
| CN205484377U (en) * | 2016-03-24 | 2016-08-17 | 三诺生物传感股份有限公司 | Detection kit |
| CN107219350A (en) * | 2017-05-31 | 2017-09-29 | 深圳市国赛生物技术有限公司 | A kind of kit |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110161266B (en) | Method for collecting liquid sample | |
| US8092385B2 (en) | Fluid access interface | |
| US6455324B1 (en) | Embossed test strip system | |
| EP1942332B1 (en) | Measuring device, measuring apparatus and method of measuring | |
| KR20140016353A (en) | Rapid identification of organisms in bodily fluids | |
| CN114585442A (en) | Detection device and reception device | |
| CN112577902A (en) | Centrifugal micro-fluidic chip for glycated albumin detection and use method thereof | |
| CN115902210A (en) | Device for detecting analyte in fluid sample | |
| CA2460073A1 (en) | Embossed test strip system | |
| CN109212233A (en) | Kit components are used in glycosylated hemoglobin detection | |
| CN109212237A (en) | Kit components are used in glycosylated hemoglobin detection | |
| CN109580973A (en) | Detection kit and biochemical immunity detection method | |
| CN214794404U (en) | Centrifugal micro-fluidic chip for glycated albumin detection | |
| CN117129671B (en) | Excrement sample collection and detection integrated device | |
| CN219104738U (en) | Test strip for immediately detecting biochemical analyzer of seminal plasma | |
| CN109212234B (en) | POCT (Point of Care testing) glycosylated hemoglobin analyzer and POCT glycosylated hemoglobin analysis system | |
| CN109212236B (en) | Kit for detecting concentration of glycosylated hemoglobin | |
| US20240189812A1 (en) | Test device | |
| EP4393594A2 (en) | Test device | |
| CN117665269A (en) | Detection device | |
| CN213302250U (en) | Device for transferring samples | |
| US20040229347A1 (en) | Embossed test strip system | |
| CN118033132A (en) | Sampling and detecting integrated device and method for helicobacter pylori antigen detection | |
| AU2023263479A1 (en) | Test device | |
| CN116908475A (en) | Detection device for detecting an analyte in a fluid sample |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190115 |