CN104569300A - Detection device for analyzed substances in liquid sample - Google Patents
Detection device for analyzed substances in liquid sample Download PDFInfo
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- CN104569300A CN104569300A CN201310502781.XA CN201310502781A CN104569300A CN 104569300 A CN104569300 A CN 104569300A CN 201310502781 A CN201310502781 A CN 201310502781A CN 104569300 A CN104569300 A CN 104569300A
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- 239000007788 liquid Substances 0.000 title claims abstract description 97
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- 238000002347 injection Methods 0.000 claims description 96
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- 238000006243 chemical reaction Methods 0.000 description 4
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 description 4
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Landscapes
- Sampling And Sample Adjustment (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention provides a detection device for analyzed substances in a liquid sample. The detection device comprises a detection cavity for containing a testing element, a sample inlet, and equipment for carrying out gas communication on gas in the detection cavity and gas outside the detection cavity by the sample inlet. A liquid sample can automatically enter the detection cavity by gas pressure difference so that a test of the analyzed substances in the liquid sample is finished.
Description
Technical field
The present invention relates to a kind of pick-up unit, the detection of illegal drug in especially a kind of tracer liquid sample.
Background technology
At present, it is more and more easier that general public obtains violated medicine, and therefore the use of violated medicine also gets more and more.In order to the fair competition of the safety and physical culture that ensure working environment, enterprise, unit and sports organization need to detect employee, athletic body fluid is such as urinated, to understand employee and whether sportsman employs violated medicine.Therefore Humoral sample Detection and Collect device is more and more, and needs to be operated by layman in common occasion.Use traditional liquid sample collection pick-up unit, exist sample easily leak and detect in because the inaccurate situation causing " Flooding " phenomenon or cannot detect of sample size, the safety detection of liquid sample and the requirement of Accurate Determining can not be met.
Device similar in existing technology, at US5,119, in 830, pick-up unit is arranged on the bowl cover of urine cup.When opening the bowl cover of urine cup, cup body for the collection of sample and can be deposited.When needing to detect, first gather sample, then the parts of a projection on bowl cover are pressed, barrier film between pick-up unit and cup body is poked, then cup body is inverted certain hour, sample along the breach crushed by projection, can enter the absorbent section of pick-up unit, thus detects from cup body.
US5,595, disclose another kind of similar pick-up unit in 187, pick-up unit is arranged on bowl cover equally.After collecting sample, when needing to detect, a valve on pressing bowl cover, makes temporary liquid enter a cavity, and cup body turns by certain hour later, and decontrol valve, the liquid sample in cavity can contact with pick-up unit, carries out detection reaction.
In addition, in open application US2004/0081581, disclose a filter paper that utilizes to absorb water pick-up unit liquid being directed to and detecting on reagent strip.This device, by upset cup body, makes sample solution contact with filter paper pads, guides sample to contact the absorbent section of reagent strip further by filter paper pads.Generally can not there is sample Overdosing problems in such device, but but there is sample deficiency causes the defect that detection is failed.
In addition, in US Patent No. 6, disclose the urine cup of the edge-on detection of a kind of transverse direction in 726,879B2, this urine cup comprises three chambeies, one is collecting chamber, one is liquid transfer chamber, and one is test chamber, and two bowl covers, one of them bowl cover is used for the sealing of collecting chamber, and whether another one bowl cover is for detecting in sample containing analyte.Introducing the direct contact liq sample in sample application zone by detecting reagent strip upstream in this patent, realizing testing goal.
This is still necessary to provide a kind of new pick-up unit, and not only can carry out quantitative sampling to the liquid sample that needs detect, handled easily simultaneously, improves the accuracy detected.
Summary of the invention
On the one hand, the invention provides so a kind of pick-up unit, comprising: for holding the test chamber of testing element; Injection port; One allows the gas in test chamber and the gas outside test chamber carry out by injection port the equipment that gas exchanges, thus allows the liquid sample outside test chamber enter into test chamber by described injection port.
Preferably, the space outside described test chamber comprises the collecting chamber for collecting fluid sample.Preferably, described liquid sample is arranged in collecting chamber.
On the one hand, the invention provides so a kind of pick-up unit, comprising: for holding the test chamber of testing element; Injection port; One allows the gas of the gas in test chamber and the collecting chamber be positioned at outside test chamber carry out by injection port the equipment that gas exchanges, thus allows the liquid sample in collection chamber enter into test chamber by described injection port.
On the one hand, the invention provides pick-up unit, comprising: for holding the test chamber of testing element; Injection port; Liquid sample collection chamber; One allows the gas of the gas in test chamber and collecting chamber carry out by injection port the equipment that gas exchanges, thus allows the liquid sample in collecting chamber enter into test chamber by described injection port.
On the one hand, the invention provides pick-up unit, comprising: liquid sample collection chamber; One for sealing the lid of collecting chamber opening, this lid comprises the test chamber for holding testing element; Injection port; One allows the gas of the gas in test chamber and collecting chamber carry out by injection port the equipment that gas exchanges, thus allows the liquid sample in collecting chamber enter into test chamber by described injection port.
In embodiments all above, the equipment that gas exchanges produces difference by the air pressure in test chamber and the air pressure outside test chamber, and the liquid sample that the air pressure of this difference makes way for injection port enters in test chamber.
In some preferred modes, described gas alternating current equipment comprises the cavity that is held gas, the volume of the cavity of this accommodation gas is transformable, such as first can diminish or can first increase, or first diminish, rear change is large, thus realizes allowing gas in test chamber by injection port and test chamber gas exchanges outward.
In some preferred modes, before the air pressure of cavity before there is volume change or in test chamber holding gas changes, injection port is detected the liquid sample sealing outside chamber.In some preferred modes, the cavity of accommodating part gas comprises elastic flexible material, by the distortion of elastic flexible material, allows the volume of the cavity holding gas change.In the mode that some are concrete, described elastic flexible material is self-elastically-deformable material, deformation of elastic element is allowed by external force, thus allow the gas in test chamber be rejected to test chamber from injection port, when after external force disappearance or partial disappearance, flexible member is returned to original state, thus allows the liquid sample in collecting chamber enter into test chamber by injection port.
Preferably, allow deformation of elastic element by external force, thus allow the gas in test chamber be rejected to collecting chamber from injection port.
In preferred mode, the cavity of this accommodation gas is communicated with test chamber gas, or the cavity holding gas is the same with the air pressure of test chamber or identical.Preferably, the cavity holding gas is communicated with test chamber gas by passage.
In some preferred modes, accommodating in test chamber can the testing element of analyte in test liquid samples.The liquid sample entered in test chamber by injection port can engaged test element.Preferably, the sample of the liquid sample entering test chamber first engaged test element applies region.In some modes, testing element comprises water-absorbing material, and liquid sample can be flowed on testing element by capillary force.
In some preferred modes, the equipment that gas exchanges comprises automatic air-exchanging motor, is reduced by the gas in test chamber by automatic air-exchanging motor, thus produces negative pressure.
In some preferred modes, described injection port is near the sample reception region of testing element.In other preferred modes, the injection port two ends that are arranged on test chamber relative to gas alternating current equipment.Preferably, gas alternating current equipment is arranged on the suction zone near testing element.
On the other hand, the invention provides the method for analyte in a kind of tracer liquid sample, the method comprises: provide pick-up unit, and this pick-up unit comprises: for holding the test chamber of testing element; Injection port; One allows the gas in test chamber and the gas outside test chamber carry out by injection port the air chamber that gas exchanges, thus allows the liquid sample outside test chamber enter into test chamber by described injection port; Liquid sample is allowed to contact injection port, thus sealing injection port; Allow the volume of air chamber reduce, the excessive gas made way in test chamber is rejected to outside test chamber by injection port; Allow the volume of air chamber again increase, the volume of increase forms negative pressure by test chamber, and the negative pressure of this formation forces the liquid sample being positioned at injection port place to enter test chamber.
In some preferred modes, the volume of described air chamber can change, and allows the air pressure of test chamber also change.In other preferred modes, the change that the air pressure of test chamber occurs forces the liquid sample being positioned at injection port to enter test chamber.In some preferred modes, preferably, the cavity of accommodating part gas comprises elastic flexible material, by the distortion of elastic flexible material, allows the volume of the cavity holding gas change.In the mode that some are concrete, described elastic flexible material is self-elastically-deformable material, deformation of elastic element is allowed by external force, thus allow the gas in test chamber be rejected to test chamber from injection port, when after external force disappearance or partial disappearance, flexible member is returned to original state, thus allows the liquid sample in collecting chamber enter into test chamber by injection port.
Preferably, allow deformation of elastic element reduce the volume of air chamber by external force, thus allow the gas in test chamber be rejected to collecting chamber from injection port.
In embodiments all above, before the described air pressure difference of generation, the injection port described in liquid sample sealing.
Beneficial effect
By pick-up unit of the present invention, the automatic acquisition of liquid sample can be realized, automatically detect and reach the quantitative object of sampling.
Accompanying drawing explanation
Fig. 1 is the perspective view of one embodiment of the invention;
Fig. 2 is the assembling perspective view of another embodiment of the present invention;
Fig. 3 is the operating process structural profile schematic diagram (device inclination) of one embodiment of the invention;
Fig. 4 is the operating process schematic diagram (cavity volume holding gas diminishes) of one embodiment of the invention;
Fig. 5 is the operating process schematic diagram (cavity volume holding gas increases again) of one embodiment of the invention;
Fig. 6 is the cross-sectional view of another embodiment of the present invention;
Description of reference numerals:
Urine cup body 200; Collecting chamber 202; The opening 201 of collecting chamber; At the bottom of collecting chamber 203; Projection 204 bottom collecting chamber; Bowl cover 100, upper cover 101, testing element 102; Upper cover chamber 109; Gas held cavity 108; The top 116 of gas held cavity; The sidewall 135 of gas held cavity; Air chamber 113; Firm banking 107; Lower cover 104; Draw-in groove 103; Injection port 120; Gas passage 114; Test chamber 115; Sample reception region 117; Surveyed area 118; Absorption region 119; Lower cover cavity 105; T-slot 140; Liquid sample 300; O-ring seal 110; Pipe 150.
Embodiment
Below to relating to structure of the present invention or these technical terms used are described further.
detect
Detect and represent chemical examination or test a kind of material or whether material exists, such as, but be not limited to this, the metabolin of chemical substance, organic compound, mineral compound, metabolism product, medicine or drug metabolite, organic organization or organic organization, nucleic acid, protein or polymkeric substance.In addition, the quantity representing test substances or material is detected.Furtherly, chemical examination also represents immune detection, chemical detection, enzyme detection etc.
sample
The sample of indication of the present invention refers to that those can be used for detecting, chemically examining or diagnose the material that whether there is interested analyte.Sample can be, such as, liquid sample, liquid sample can comprise blood, blood plasma, serum, urine, saliva and various juice, can also comprise the liquid solution that solid sample and semi-solid samples are formed after anticipating.The sample that collection comes may be used for the methods such as immune detection, chemical detection, enzyme detection and detects whether there is analyte.
analyte
Any analyte can be analyzed by apparatus and method of the present invention.Analyte can detect in any liquid or liquefied sample, such as urine, saliva, saliva, blood, blood plasma, or serum.
Analyte can also be some haptens materials, and these haptens comprise drugs (as drug abuse)." drug abuse " (DOA) refers to that non-medical destination uses medicine (usually playing paralysis neural).This device is used also to may be used for detecting and belong to medical usage but the detection of easy overdose, as tricyclic antidepressant (imipramine or analog) and Paracetamol etc.Can resolve into different small-molecule substances after these medicines are absorbed by the body, these small-molecule substances are present in the body fluid such as blood, urine, saliva, sweat or part body fluid exists above-mentioned small-molecule substance.
pick-up unit
In a concrete embodiment, pick-up unit provided by the invention comprises: for holding the test chamber of testing element, the air chamber of injection port and variable volume, allows the gas in test chamber be exchanged by injection port and extraneous liquid sample by the stereomutation of air chamber.In a preferred mode, the air chamber of variable volume is made up of elastic flexible material, and air chamber is communicated with the strong gas of detection by gas passage.When the volume of air chamber changes time, allowing the gas volume in whole test chamber also there occurs change, in order to maintain test chamber and outside gas or air pressure balance, just needing the needs being reached inside and outside air pressure balance by injection port exchanging gas.
In addition, in test chamber, can testing element be placed, certainly, also can not place testing element.Testing element can be arranged in test chamber in advance, also can in the test chamber that analyte is placed testing element before detecting temporarily in sample.
When operation, in time including test-strips in test chamber, allow air chamber be in a stable state, now air chamber is communicated with test chamber gas, and their gas volume or the air pressure of inside remain unchanged.After gathering liquid, allow injection port be embedded under liquid sample liquid level, or injection port is sealed by liquid sample, now, test chamber and air chamber form a closed environment.In this time, allowing the volume of air chamber reduce, is even 0, and now, the gas being previously positioned at air chamber is pressed into test chamber, allows the gas in test chamber increase, thus pressure increase.The pressure increased can overcome the pressure of hydraulic seal injection port, allows a part of gas be rejected to outside test chamber by injection port.At this time, if increase the volume of air chamber, allow test chamber become negative pressure, in order to overcome negative pressure, partially liq enters test chamber by injection port, contacts with test-strips, completes the test (Fig. 6) of analyte in sample matter.
Certainly, operation that also can be such: in time including test-strips in test chamber, allow air chamber be in a stable state, now air chamber is communicated with test chamber gas, and their gas volume or the air pressure of inside remain unchanged.Allowing the volume of air chamber reduce, is even 0, now, the gas being previously positioned at air chamber is pressed into test chamber, allows the gas in test chamber increase, thus pressure increase, the pressure increased is rejected to outside by injection port, and the external and internal pressure of such test chamber reaches balance.After gathering liquid, allow injection port be embedded under liquid sample liquid level, or injection port is by hydraulic seal, now, test chamber and air chamber form a closed environment.At this time, if increase the volume of air chamber, test chamber is allowed to become negative pressure, in order to overcome negative pressure, partially liq enters test chamber (achieving the fluid exchange outside the gas-detecting cavity in test chamber) by injection port, contacts, complete the test of analyte in sample matter with test-strips.
In some preferred modes, the volume change of air chamber completes by resilient material, such as, because portion gas chamber is made up of resilient material, when resilient material is subject to external force, the volume of air chamber diminishes relative to when not being subject to external force, and unnecessary gas is pressed into test chamber.On the contrary, after external force disappears, due to the flexible needs of self-recovery, the volume of air chamber increases, and the space of increase allows test chamber create negative pressure, and the negative pressure of generation be entered in test chamber by injection port by can be allowed outside liquid sample and contact with testing element.Concrete principle and method as previously mentioned described in.Diminishing of volume can be 10% of initial volume, is at least 20%, is at least 35-80%, be at least 40-95%, can for allowing the volume of air chamber be reduced to 0.After the volume of air chamber is diminished, the gas in test chamber is corresponding to be got rid of by injection port.In time having hydraulic seal injection port, if now increase the volume of air chamber, allow test chamber become negative pressure, liquid sample enters into test chamber by injection port.
In some modes, the volume change of air chamber completes by elastic flexible material, and external force can automatically automatically be added by external unit and automatically disappear.The volume change of air chamber also can be reduced by piston like motion or increase volume.When the volume of air chamber changes time, this change allows the gas volume in test chamber or gaseous tension also change thereupon, this change realizes the exchange of gas and liquid by injection port, thus realize liquid sample and enter test chamber, and then can engaged test element, complete chemical examination or the detection of analyte in liquid sample.
Resilient material can be selected from rubber, latex or other conventional resilient material to realize function of the present invention.In some preferred modes, resilient material is latex.
In a concrete embodiment, pick-up unit of the present invention comprises: lid, lid comprises the test chamber holding testing element, the air chamber of injection port and variable volume, the gas realized in test chamber by the volume change of air chamber is exchanged by the liquid sample outside injection port and test chamber, thus allows liquid enter test chamber.Such as, as shown in Figure 3.Lid 100 comprises the test chamber 115 holding testing element, and injection port 120, and the air chamber 113 of variable volume, air chamber is communicated with test chamber gas, such as, be interconnected by gas passage 114.Preferably, this pick-up unit also comprises the collecting chamber for collecting liquid sample, and lid is used to the opening sealing this collecting chamber.When operation, first go to collect liquid sample in collecting chamber 202 with collecting chamber, such as, collect in the collecting chamber in cup 200.Then seal the opening 201 of collecting chamber with lid 100, now, the injection port on lid not with liquid comes into contact.Then, inclination cup 200, allows liquid sample seal injection port 120, now, because the volume of test chamber and air chamber remains unchanged substantially, does not almost have liquid to enter into test chamber (Figure 30.Press with finger air chamber 113, allow its smaller volume, unnecessary gas is pressed in test chamber 115, causes test chamber pressure increase, and unnecessary gas is rejected to (Fig. 4) in test chamber by injection port 120.Then external force disappears, because air chamber is made up of resilient material, after external force disappears, have the ability recovering original state, in this time, the volume of air chamber 113 increases again, thus allow test chamber become negative pressure, in order to equalized pressure, liquid sample enters into test chamber 115 by injection port 120, contacts (Fig. 5) with the testing element 102 in test chamber.In a preferred mode, the diameter of injection port is 0.1-10 millimeter, 0.01-0.5 millimeter.Or be 10-500 micron; Allow this injection port have certain surface tension, there is no gas or fluid exchange between test chamber and collecting chamber before, reduce liquid, even avoid liquid to enter (Fig. 3) in test chamber.In other preferred modes, in one end of injection port 120 and the place being positioned at test chamber 115 is "T"-shaped mouth (cross section view), and injection port is in one end of a pipe 150, and the other end connects " T " word mouth.Two annexes being positioned at " T " word mouth are sample reception regions of testing element.Like this, the liquid sample 300 being entered test chamber 115 by injection port 120 goes out to collect in " T " rim of the mouth, enables the liquid sample of smaller size smaller fully contact with sample reception region like this.
In some preferred modes, lid comprises upper cover body 101 and lower cover 104, and the testing element 102 between upper and lower lid, in the test chamber 115 that this testing element is formed between upper and lower covers.The upper cover chamber 109 of a depression is formed at upper cover place, lower cover is formed corresponding caveli body 105, upper cover chamber 109 coordinates with lower cover cavity 105, a pedestal or firm banking 107 are set between them, the opening edge of flexible gas container cavity 108 is along being arranged in pedestal, reserve the passage 114 of a gas circulation at flexible gas container cavity in test chamber, this passage 114 connects gas held chamber 108 and test chamber 115 simultaneously.Gas held cavity 108 comprises top 116 and the sidewall 135 of gas held, and top and sidewall define air chamber 113.The top 116 of container cavity 108 and sidewall 135 are elastic material formation.This top 116 is not upwardly higher than the plane in the upper cover chamber 109 of upper cover.Like this, when in use, external force can be applied to the top 116 of gas held cavity 108, allow gas held cavity generation deformation, allow the volume in gas held chamber 113 change, such as, become large or diminish.In preferred mode, after this resilient material is subject to external force, cavity 113 is allowed to there occurs the change of volume, if after external force disappearance, resilient material can get back to original state voluntarily, thus allows the volume of air chamber change again.Such as, when original state (Fig. 3), top is positioned in upper cover cavity, has layer protecting film 112 to cover at top.The effect of this diaphragm is that the top preventing and treating unexpected extrusion chamber 113 allows the volume of cavity 113 change.When needing operation, open diaphragm 112, then the top 116 of firmly extruding air chamber 113, allow the smaller volume (Fig. 4) of air chamber.After the smaller volume of such air chamber 113, the gas or the air that were originally positioned at air chamber 113 are discharged to test chamber 115 by passage 114.It is essence rigidity that material due to test chamber is formed, and hardly (almost not occurring at such pressures) elastic deformation occurs at such pressures.Air pressure in such test chamber 115 raises, and in order to keep the air pressure balance inside and outside test chamber, unnecessary gas gets rid of the outside of test chamber 115 by injection port 120, such as, be rejected in collecting chamber 202.In time eliminating external force, get back to initial elasticity because top 116 and sidewall 135 have, like this, the volume of air chamber becomes again large, allow in test chamber 115 and create negative pressure, thus the liquid sample 300 making way for injection port 120 enters test chamber 115 and contacts with the testing element 102 in it.Injection port is arranged on the another side of lid, and opposes with air chamber and arrange.
In some modes, the test chamber in lid comprises one or more draw-in groove 103, arrange a testing element 102 in each draw-in groove, meanwhile, each testing element comprises sample reception region 117, surveyed area 118 and absorption region 119.In preferred mode, position corresponding with surveyed area in upper cover body 101 is transparent, directly by the reaction on transparent place observation surveyed area or reaction result, thus can obtain test result like this.
Except describing above, test chamber also can be positioned at the sidewall of collecting chamber.In addition, air chamber and test chamber and injection port are positioned at the sidewall of collecting chamber, are connected as a single entity with collecting chamber.Certainly, in other mode, collecting chamber 202 is not the necessary part of the present invention.
In other mode, the bottom of collecting chamber 202 is projection 204, and this prominent role is when liquid sample is little time, and liquid sample can be allowed to collect when cavity tilts, the height of injection port can be reached, can meet like this and allow liquid sample seal injection port.
testing element
Testing element can select the test strip of cross flow, and it can detect multiple analyte.Certainly, other suitable testing elements also can be used in the present invention.
Various testing element can be grouped together and apply in the present invention.A kind of form is Test paper.Test paper for the analyte (as drugs or the metabolin showing health) in analyzing samples can be various forms, as immunoassays or chemico-analytic form.Test paper can adopt the analytical model of non-competing method or competition law.Test paper comprises the absorbent material that has sample application zone, reagent area and test section.Add sample to sample application zone, flow to reagent area by capillarity.In reagent area, if there is analyte, sample is combined with reagent.Then sample continues to flow to detection zone.Other reagent, as being fixed on detection zone with the molecule of the Non-specific combination of analyte.Analyte (if existence) in these reagent and sample reacts and analyte is combined in this district, or is combined with the some reagent of reagent area.Label for showing detection signal exists and reagent area or the mark zone that is separated.
Typical non-competing method analytical model is that signal will produce if containing analyte in sample, if do not comprise analyte, does not just produce signal.In competition law, if analyte is not present in sample, signal produces, if there is analyte, does not then produce signal.
Testing element is Test paper, the material can selected water suction or not absorb water.Test paper can comprise multiple material for liquid sample transmission.Wherein a kind of material of Test paper can cover on another material, as filter paper covers on nitrocellulose filter.A district of Test paper can select one or more materials, and one or more materials that another district selects other different.Test paper can be attached on certain holder or hard surface for improving the intensity being affectedly bashful Test paper.
Analyte is detected by signal generating system, as utilized one or more enzymes with this analysis thing generation specific reaction, utilize as aforementioned method specific combinating substance is fixed on Test paper, the composition of one or more signal generating systems is fixed on the analyte detection zone of Test paper.The material producing signal can in sample application zone, reagent area, or detection zone, or on whole Test paper, this material can be full of on one or more materials of Test paper.Solution containing signal thing is added to the surface of test paper or one or more materials of test paper are immersed in the solution containing signal thing.Make to add the test paper drying containing signal thing solution.
Each district of Test paper can arrange in the following manner: sample application zone, reagent area, detection zone, control zone, determines the whether adulterated district of sample, liquid sample uptake zone.After control zone is positioned at detection zone.All districts can be arranged at only with on an a kind of test paper of material.May also be not same district and adopt different materials.Each district can directly contact with liquid sample, or different district is according to the direction arrangement of liquid sample flowing, is connected by the end in each district and overlapping with the front end in another district.Material used can be the good material of water absorptivity as filter paper, glass or nitrocellulose filter etc.Test paper also can adopt other forms.
embodiment
Be described in detail the specific embodiment of the present invention by reference to the accompanying drawings now, this explanation is not carry out any restriction to the claims in the present invention, and is used to illustrate how to realize the present invention, belongs to a concrete mode of the present invention.
Fig. 1-5 is a specific embodiment of the present invention.Such as shown in Fig. 1, pick-up unit comprises lid, and the test chamber formed in lid and hold the cavity of gas and injection port.Lid comprises upper cover body 101 and lower cover 104, and the testing element 102 between upper and lower lid, in the test chamber 115 that this testing element is formed between upper and lower covers.The upper cover chamber 109 of a depression is formed at upper cover place, lower cover is formed corresponding caveli body 105, upper cover chamber 109 coordinates with lower cover cavity 105, a pedestal or firm banking 107 are set between them, the opening edge of flexible gas container cavity 108 is along being arranged in pedestal, reserve the passage 114 of a gas circulation at flexible gas container cavity in test chamber, this passage 114 connects gas held chamber 108 and test chamber 115 simultaneously.Gas held cavity 108 comprises top 116 and the sidewall 135 of gas held, and top and sidewall define air chamber 113.The top 116 of container cavity 108 and sidewall 135 are elastic material formation.This top 116 is not upwardly higher than the plane in the upper cover chamber 109 of upper cover.Pick-up unit is formed after lid assembling.
Meanwhile, provide one to collect the container of liquid sample, such as cup 200, this cup comprises opening 201 and collecting chamber 202 and bottom 203, and lid can be used for airtight or sealed open 201.Lid also arranges O-ring seal 110.When in use, first liquid sample, such as urine is collected with collecting cup.Then cover cap is combined on opening, then tilt whole device, as Fig. 3.Like this, collect in the collecting chamber of liquid sample in cup, and flood injection port 120, or sealing injection port.In this time, whole test chamber is sealed as a closed space, and because external and internal pressure is consistent, liquid sample enters in test chamber 115 hardly.In this time, open diaphragm 112, allow the top 116 with elastically-deformable air chamber expose, with finger or exterior mechanical instrument extruding top 116, allow the smaller volume of air chamber 113.Allow exist with air chamber in 113 gas enter in test chamber 115 by passage 114, air pressure in such test chamber raises, and the gas that the air pressure of rising is unnecessary is rejected to (Fig. 4) in collecting chamber by injection port 120.Then external force is eliminated, because resilient material has the ability recovering virgin state, the volume of air chamber is allowed to become large, becoming large volume needs more gas to enter air chamber, the gas in test chamber 115 is allowed to enter in air chamber like this, test chamber is allowed to become negative pressure, because pressure reduces, and the pressure of test chamber is higher than the pressure in test chamber, like this, liquid sample to enter in test chamber and contacts with the testing element in test chamber, and liquid sample enters test chamber and balances pressure differential between test chamber and collecting chamber.This completes the detection of testing element to analyte in liquid sample.Can be obtained by transparent upper cover body or the testing result in read test region.
Claims (32)
1. the pick-up unit of analyte in liquid sample, comprising: for holding the test chamber of testing element; Injection port; One allows the gas in test chamber and the gas outside test chamber carry out by injection port the equipment that gas exchanges.
2. pick-up unit according to claim 1, wherein, described gas alternating current equipment produces pressure differential by the air pressure in test chamber and the air pressure outside test chamber, and this pressure differential forces the liquid sample outside test chamber to enter into test chamber.
3. pick-up unit according to claim 2, wherein, before producing pressure differential in described test chamber, injection port is sealed by the liquid sample be positioned at outside test chamber.
4. the liquid sample be positioned at according to the pick-up unit one of claim 2-3 Suo Shu, wherein, outside test chamber is positioned at the collecting chamber collecting fluid sample.
5. according to the pick-up unit one of claim 2-4 Suo Shu, wherein, described gas alternating current equipment comprises the air chamber of the accommodation gas of variable volume.
6. pick-up unit according to claim 5, wherein, described air chamber is communicated with by gas passage with test chamber.
7. pick-up unit according to claim 5, wherein, the volume change of described gas cavity allows the air pressure in test chamber change, thus cause in described test chamber with the draught head outside test chamber.
8. pick-up unit according to claim 5, wherein, described air chamber comprises elastic deformation material.
9. pick-up unit according to claim 5, wherein, it is large or diminish that described variable volume comprises the change of volume.
10. pick-up unit according to claim 5, wherein, described variable volume comprises the minimizing from initial volume to volume, then becomes again large from the volume after minimizing.
11. pick-up units according to claim 8, wherein, described elastic flexible material is self-elastically-deformable material, deformation of elastic element is allowed by external force, thus allow smaller volume in air chamber, thus allowing the air pressure in test chamber increase, the gas that the air pressure of increase is made way in test chamber is rejected to test chamber from injection port.
12. pick-up units according to claim 11, wherein, when after external force disappearance or partial disappearance, flexible member is returned to original state, the volume of air chamber is large from little change, and allow in test chamber and form negative pressure, negative pressure forces liquid sample to be entered in test chamber by injection port.
13. according to the pick-up unit one of claim 1-11 Suo Shu, wherein, comprises the testing element of analyte in for test liquid samples in test chamber.
14. pick-up units according to claim 13, wherein, the sample application zone of described testing element is near injection port.
The pick-up unit of analyte in 15. 1 kinds of liquid samples, comprising: liquid sample collection chamber; One for sealing the lid of collecting chamber opening, this lid comprises the test chamber for holding testing element; Injection port; One is held the air chamber of gas, and allow the air pressure in test chamber and the air pressure outside test chamber produce pressure differential by the volume change of air chamber, this pressure differential forces the liquid sample in collecting chamber to enter into test chamber by described injection port.
16. pick-up units according to claim 15, wherein, before the draught head described in being formed, liquid sample sealing injection port.
17. pick-up units according to claim 15, wherein, described injection port and air chamber are arranged on the both sides of lid respectively.
18. pick-up units according to claim 15, wherein, the volume of air chamber is variable, and the change of volume creates described draught head.
19. pick-up units according to claim 18, wherein, described air chamber comprises elastic deformation material.
20. pick-up units according to claim 19, wherein, it is large or diminish that described variable volume comprises the change of volume.
21. pick-up units according to claim 20, wherein, described variable volume comprises the minimizing from initial volume to volume, then becomes again large from the volume after minimizing.
22. pick-up units according to claim 21, wherein, described elastic flexible material is self-elastically-deformable material, deformation of elastic element is allowed by external force, thus allow smaller volume in air chamber, thus allowing the air pressure in test chamber increase, the gas that the air pressure of increase is made way in test chamber is rejected to test chamber from injection port.
23. pick-up units according to claim 21, wherein, when after external force disappearance or partial disappearance, flexible member is returned to original state, the volume of air chamber is large from little change, and allow in test chamber and form negative pressure, negative pressure forces liquid sample to be entered in test chamber by injection port.
The method of analyte in 24. 1 kinds of tracer liquid samples, the method comprises:
There is provided pick-up unit, this pick-up unit comprises: for holding the test chamber of testing element; Injection port; One allows the gas in test chamber and the gas outside test chamber carry out by injection port the air chamber that gas exchanges, enters into test chamber by gas exchanges by the liquid sample outside test chamber by described injection port; Allow the volume of air chamber reduce, the excessive gas made way in test chamber is rejected to outside test chamber by injection port;
Allow the volume of air chamber again increase, the volume of increase forms negative pressure by test chamber, and the negative pressure of this formation forces the liquid being positioned at injection port place to enter test chamber.
25. methods according to claim 24, wherein, the excessive gas in test chamber be rejected to test chamber outer before or allow the volume of air chamber again increase again, the volume of increase is by before forming negative pressure in test chamber, liquid sample is allowed to contact injection port, thus sealing injection port.
26. according to the method one of claim 24-25 Suo Shu, and wherein, the volume of air chamber is variable.
27. methods according to claim 26, wherein, the change of air chamber volume allows in test chamber and produces draught head outward with test chamber, and described draught head forces liquid sample to be entered in test chamber by injection port.
28. methods according to claim 27, wherein, described air chamber comprises elastic deformation material
29. methods according to claim 28, wherein, described elastic flexible material is self-elastically-deformable material, deformation of elastic element is allowed by external force, thus allow smaller volume in air chamber, thus allowing the air pressure in test chamber increase, the gas that the air pressure of increase is made way in test chamber is rejected to test chamber from injection port.
30. methods according to claim 29, wherein, when external force disappears or after partial disappearance, flexible member is returned to original state, the volume of air chamber is large from little change, and allow in test chamber and form negative pressure, negative pressure forces liquid sample to be entered in test chamber by injection port.
31. according to the method one of claim 24-30 Suo Shu, wherein, comprises the testing element of analyte in for test liquid samples in test chamber.
32. methods according to claim 31, wherein, the sample application zone of described testing element is near injection port.
Priority Applications (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310502781.XA CN104569300B (en) | 2013-10-22 | 2013-10-22 | The detection means of analyte in a kind of liquid sample |
| PCT/CN2014/088102 WO2015058612A1 (en) | 2013-10-22 | 2014-10-05 | A testing device for testing analytes in liquid samples |
| EP14856723.3A EP3060343A4 (en) | 2013-10-22 | 2014-10-05 | A testing device for testing analytes in liquid samples |
| CN201480055239.7A CN105612001B (en) | 2013-10-22 | 2014-10-05 | The detection device of analyte in a kind of liquid sample |
| US15/030,852 US10495635B2 (en) | 2013-10-22 | 2014-10-05 | Testing device for testing analytes in liquid samples |
| HK16110928.8A HK1222597A1 (en) | 2013-10-22 | 2016-09-15 | A testing device for testing analytes in liquid samples |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310502781.XA CN104569300B (en) | 2013-10-22 | 2013-10-22 | The detection means of analyte in a kind of liquid sample |
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| Publication Number | Publication Date |
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| CN104569300A true CN104569300A (en) | 2015-04-29 |
| CN104569300B CN104569300B (en) | 2017-07-28 |
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| CN201310502781.XA Expired - Fee Related CN104569300B (en) | 2013-10-22 | 2013-10-22 | The detection means of analyte in a kind of liquid sample |
| CN201480055239.7A Expired - Fee Related CN105612001B (en) | 2013-10-22 | 2014-10-05 | The detection device of analyte in a kind of liquid sample |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201480055239.7A Expired - Fee Related CN105612001B (en) | 2013-10-22 | 2014-10-05 | The detection device of analyte in a kind of liquid sample |
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| CN (2) | CN104569300B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109085159A (en) * | 2017-08-21 | 2018-12-25 | 上海荒岛科技有限公司 | A kind of substrate and detection card |
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| WO2017201723A1 (en) * | 2016-05-27 | 2017-11-30 | 卓金星 | Microorganism detection system |
| US20210170396A1 (en) * | 2018-01-16 | 2021-06-10 | Hewlett-Packard Development Company, L.P. | Fluid testing |
| CN116990519B (en) * | 2023-09-28 | 2023-11-28 | 山东省立第三医院 | Portable blood sugar detector container |
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Also Published As
| Publication number | Publication date |
|---|---|
| CN105612001A (en) | 2016-05-25 |
| CN104569300B (en) | 2017-07-28 |
| CN105612001B (en) | 2018-06-26 |
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