CN204789228U - Whole blood blood coagulation and adhesion work can detectors - Google Patents
Whole blood blood coagulation and adhesion work can detectors Download PDFInfo
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- CN204789228U CN204789228U CN201520514713.XU CN201520514713U CN204789228U CN 204789228 U CN204789228 U CN 204789228U CN 201520514713 U CN201520514713 U CN 201520514713U CN 204789228 U CN204789228 U CN 204789228U
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Abstract
The utility model discloses a whole blood blood coagulation and adhesion work can detectors, hematoid cup, a rotary device who links to each other with the detection cup, light wave generator, light wave receiver and the light tight casing of detecting is waited to detect including being used for placing by the detector, detect the inside bottom of cup and be equipped with the printing opacity lagging, leave the space between lagging and the detection cup inner wall, rotary device is used for the drive to detect the cup around self center pin rotation, light wave generator and light wave receiver opposite direction set are detecting the cup both sides and are being two sets of at least for detect and see through the light wave intensity that detects the cup, it all sets up in light tight casing to detect cup, light wave generator and light wave receiver. The utility model discloses can accomplish the detection on an instrument with blood blood coagulation function and adhesion work, check -out time has been saved to the operating is simplified procedure.
Description
Technical field
The utility model belongs to clinical medical inspection field, particularly a kind of whole blood blood coagulation and adhesion function detector.
Background technology
Blood coagulation function, adhesion function are the critical functions of blood, and these functions change and there is close relationship with thrombus, hemorrhage and some other blood disease.Existing coagulo meter is by turbidimetry, and the principle utilizing blood turbidity in coagulation process to raise suddenly designs, and only evaluates blood coagulation function, does not have the detection of blood adhesion function.Existing platelet adhesiveness test carries out external test to hematoblastic adhesiveness, blood platelet has and adheres to non-physiologic surface of contact as the critical function on the materials such as glass, the collagenous fibres exposed after vascular wall breaks have special compatibility to blood platelet, make platelet adhesion reaction sex change, Adherence of Platelet lowers, and easily occurs hemorrhage; Adherence of Platelet increases, and thrombus is easy to be formed.
Because blood coagulation function and adhesion function have detected respectively on coagulo meter and platelet adhesion reaction instrument, because this increasing the consumption of blood sample, add the workload of operator, work efficiency is lower.
Utility model content
The object of utility model is to provide a kind of whole blood blood coagulation and adhesion function detector.
The technical scheme realizing the utility model object is:
A kind of whole blood blood coagulation and adhesion function detector, the whirligig, light wave generator, Lightwave receiver and the light tight housing that comprise the detection cup for placing blood sample to be detected, are connected with detection cup;
Described detection cup inner bottom is provided with printing opacity lagging, leaves space between lagging and detection cup inwall;
Described whirligig rotates around its central axis for driving detection cup;
Described light wave generator and Lightwave receiver subtend are arranged on and detect cup both sides and be at least two groups, for detecting the light wave intensity through detecting cup;
Detect cup, light wave generator and Lightwave receiver to be all arranged in light tight housing.
Compared with prior art, remarkable result of the present utility model is: (1) the utility model achieves blood coagulation function and adhesion function completes detection on an instrument, original respective independently testing process is effectively integrated, simplifies procedures, save detection time; (2) the utility model only needs a blood sample single injected sampling just can complete blood coagulation function and adhesion function detection, reduces the restriction of sample collection difficulty; (3) detector structure of the present utility model is simple, detects the waste liquid produced few, is conducive to environmental protection.
Accompanying drawing explanation
Detector side schematic view when Fig. 1 is initial time blood sample ot-yet-hardened.
Fig. 2 is that blood sample has solidified and adhered to, and rotates extremely higher than detector side schematic view during former blood sample surface level with detection cup.
Fig. 3 be two groups of light wave generators and Lightwave receiver to be separately positioned on above baffle plate and baffle position time detector diagrammatic cross-section.
Fig. 4 is that conventional plasma detection method and the utility model detect TT results relevance analysis chart.
Fig. 5 is that conventional plasma detection method and the utility model detect PT results relevance analysis chart.
Fig. 6 is that detection method of the present utility model (X) detects same sample TT result consistency analysis figure with blood plasma method (Y).
Fig. 7 is that detection method of the present utility model (X) detects same sample PT result consistency analysis figure with blood plasma method (Y).
Fig. 8 be two groups of light wave generators and Lightwave receiver to be separately positioned on below baffle plate and baffle position time detector diagrammatic cross-section.
Fig. 9 be two groups of light wave generators and Lightwave receiver to be separately positioned on above baffle plate and below baffle plate time detector diagrammatic cross-section.
Embodiment
Composition graphs 1, a kind of whole blood blood coagulation and adhesion function detector, the whirligig, light wave generator, Lightwave receiver and the light tight housing that comprise the detection cup for placing blood sample to be detected, are connected with detection cup;
Described detection cup inner bottom is provided with printing opacity lagging, and lagging is arranged with detection cup bottom vertical, and leave space between lagging both sides and detection cup inwall, space is not more than 1mm;
Described whirligig rotates around its central axis for driving detection cup;
Described light wave generator and Lightwave receiver subtend are arranged on and detect cup both sides and be at least two groups, for detecting the light wave intensity through detecting cup;
Detect cup, light wave generator and Lightwave receiver to be all arranged in light tight housing.
Further, when light wave generator and Lightwave receiver quantity are two groups, primary importance and the second place can be arranged on, or be arranged on the second place and the 3rd position, or be arranged on primary importance and the 3rd position.
Described primary importance is that light wave generator and Lightwave receiver subtend are arranged on the left and right sides of detecting cup and higher than the position of blood sample liquid level to be detected, light wave is positioned at above lagging simultaneously;
The described second place is that light wave generator and Lightwave receiver subtend are arranged on the left and right sides of detecting cup and higher than the position of blood sample liquid level to be detected, light wave is through lagging simultaneously;
Described 3rd position is that light wave generator and Lightwave receiver subtend are arranged on the left and right sides of detecting cup and lower than the position of blood sample liquid level to be detected, light wave is positioned at below lagging simultaneously.
Further, described detector also comprises the detecting position with heated at constant temperature function, and described detecting position parcel is arranged on the outside detecting cup.
Described detector also comprises timer, for recording the blood sample coagulation time to be detected.
Described detection cup axis becomes 3-80 ° of angle with surface level, and becomes rotating along its central axis of constant speed when detecting.
Described light wave generator is the light wave generator of more than 700nm wavelength.
Below in conjunction with specific embodiment, the utility model is described further.
Embodiment 1
Composition graphs 1, a kind of whole blood blood coagulation and adhesion function detector, comprise whirligig 1, coupling arrangement 2, pick-up unit;
Described whirligig 1 is connected with pick-up unit by coupling arrangement 2, carries out unidirectional constant speed rotary or reciprocating rotation at the uniform velocity for driving detection cup around its central axis; Described pick-up unit comprises and detects cup 6, detecting position 3, first light wave generator 8a with heating function, the first Lightwave receiver 9a, the second light wave generator 8b and the second Lightwave receiver 9b; Detect bottom cup and be provided with printing opacity lagging 10, lagging is arranged with detection cup bottom vertical, and leave space between lagging both sides and detection cup inwall, space is not more than 1mm, and detecting position 3 parcel is arranged on and detects cup 6 periphery;
Composition graphs 3, first light wave generator 8a and the first Lightwave receiver 9a subtend is arranged on the left and right sides of detecting device and light path is positioned at above baffle plate, second light wave generator 8b and the second Lightwave receiver 9b subtend are arranged on the left and right sides of detecting device and light path is alignd with baffle position, wherein, the first light wave generator 8a, the first Lightwave receiver 9a, the second light wave generator 8b and the second Lightwave receiver 9b are all higher than whole blood blood sample 5 liquid level time initial.
Described detection cup is under whirligig 1 drives, and by coupling arrangement 2, detect cup 6 with central shaft x-x ' for axle center rotates, z-z ' is transverse axis, and x-x ' axle becomes 3-80 degree angle with z-z ' axle; The invariablenes turning speed of described whirligig is set to often revolve the 2s that turns around.
Check point 7 be arranged on detect cup both sides, detecting position is provided with through hole in the position of check point 7, be convenient to infrared light through.Light wave generator and Lightwave receiver are separately positioned on the both sides of check point 7, and when only having detection cup or inside only to have thin layer blood film, infrared ray can pass through to detect cup and blood thin layer, therefore detecting device can obtain stronger detection signal, maximum 4300mV.During detection, detecting position and detection cup should be the states isolated with extraneous light.Add the clotting reagent time to the time length that blood clotting is formed by record, the coagulation function can evaluating tested blood sample is strong and weak.
The present embodiment detects cup axis and becomes 25 degree of angles with surface level, and both sides are respectively provided with one group of detecting device, composition graphs 3 above detection cup baffle plate both sides and baffle plate, and the testing process of the present embodiment is:
Step 1, patient whole blood's sample is gathered with citrate anticoagulation pipe, before detecting quantitative citrate anticoagulation whole blood (0.2ml) added to and detect in cup, and in blood sample, add 0.2ml physiological saline sample 1:1 is diluted, to improve mobility and the detection sensitivity of sample blood;
Step 2, a detecting position tilted is placed in by detecting cup, this detecting position axis becomes 25 degree of angles with surface level, and has heated constant temperature function and drive detection cup along the function of cup central shaft rotary motion, and during detection, detecting position and detection cup should be the states isolated with extraneous light;
Step 3, detect time wave producer to continue to send infrared light, although now detect cup to continue to rotate with its central axis, but because blood sample is also in liquid state, can not rotate with detection cup, now at the infrared detector continuous reception infrared light detecting the setting of cup offside relevant position, peak signal is 4300mV.
Add clotting reagent, as blood sample coagulation adheres to, then blood clot sticks to the bottom regional area detecting cup, and with the rotary motion of detection cup, as shown in Figure 2, when blood clot rotates to the light path position between infrared light and detecting device, detecting device can detect lightwave signal change 20% and more than, or lightwave signal change difference is greater than 100mV, if the light signal received is B, then after this detection cup rotate a circle interior first Lightwave receiver 9a receive light signal become 4300-B-4300 regularity change, second Lightwave receiver 9b receives light signal and becomes the change of 4300-B-4300-B-4300 regularity,
As blood sample coagulation does not adhere to, along with detection cup rotates, due to the effect of lagging, blood clot gets back to bottom after being taken to the second detection position by lagging, but can not arrive and detect above cup, therefore the light signal that the first Lightwave receiver 9a receives is always 4300mv, changes detecting the cup regularity that signal that interior Lightwave receiver 9b detects becomes 4300-B-4300 by initial 4300mv that rotates a circle;
Do not adhere to as blood sample does not solidify, blood sample is in all the time and detects bottom cup, then Lightwave receiver 9a and 9b receives the light signal that light wave generator 8a and 8b launch and be always 4300mv.
Record adds the time of clotting reagent and the time of blood clotting formation, detects the blood sample clotting time thus, thus judges the coagulation function of blood sample, and change formation time is shorter then illustrates that corresponding function is stronger.
Table 1 detecting device to be separately positioned on above baffle plate and baffle position place and detect the result (light signal unit: mv) that cup rotates a circle after certain hour
| Receiver (9a) | Receiver (9b) | Testing result | |
| Light signal 1 | 4300-B-4300 | 4300-B-4300-B-4300 | Solidify adhesion |
| Light signal 2 | 4300 | 4300-B-4300 | Solidify and do not adhere to |
| Light signal 3 | 4300 | 4300 | Do not solidify and do not adhere to |
Classical Clinical significant of coagulation function detection method and the utility model is adopted to detect same sample results comparison test.
The same sample of table 2 compares with the utility model testing result (unit: second) by classical blood plasma detection method respectively
Classical blood plasma detection method and the utility model is adopted to detect TT (thrombin time), PT (prothrombin time) respectively, classical blood plasma detection method is: the citrate anticoagulation whole blood of collection is gone out blood plasma through centrifuging, add in detection cup by being separated the blood plasma obtained according to detection requirement, constant-temperature incubation, then add certain TT or PT reagent, the sample clotting time is determined in the change adding transmitted light or scattered light in rear reaction system by detecting reagent.Detect the repeated CV value of same sample, TT (thrombin time) is respectively: classical blood plasma detection method is 1.23%, and the utility model result is 1.10%; PT (prothrombin time) is respectively: classical blood plasma detection method is 1.55%, and the utility model is 1.51%.Detect the result mean value of same sample, TT is respectively: classical blood plasma method 14.265 seconds, and the utility model is 14.245 seconds; PT is respectively: classical blood plasma method 12.295 seconds, the utility model 12.30 seconds.
Result illustrates that the utility model detects the TT (thrombin time) that obtains and PT (prothrombin time) and all has good repeatability and consistance.
Classical Clinical significant of coagulation function detection method and the utility model is adopted to detect different sample results comparison test.
Table 3 different specimens compares with the utility model testing result (unit: second) by classical blood plasma detection method respectively
1. correlation analysis
Fig. 4 and Fig. 5, two kinds of distinct methods testing result correlativity: R
tT=0.974>=0.95, R
pT=0.970>=0.95, illustrate that two kinds of method testing result correlativitys are good.
2. consistency analysis (Bland-altman analysis)
As shown in Figure 6, compared with the utility model (X) detects TT (Y) with classical blood plasma method, the point of 100% is all in 95% consistance interval, and two methods and results consistance are good.
As shown in Figure 7, compared with the utility model (X) detects PT (Y) with classical blood plasma method, the point of 100% is all in 95% consistance interval, and two methods and results consistance are good.
Conclusion two kinds of methods measure same sample respectively, its testing result correlativity and consistance good.Two kinds of detection method results are equivalent, can mutually replace.
Embodiment 2
Composition graphs 8, the present embodiment and embodiment 1 be distinguished as two groups of light wave generators and receiver is separately positioned on both sides below baffle plate both sides and baffle plate, namely the second light wave generator 8b and the second Lightwave receiver 9b subtend are arranged on and detect the cup left and right sides and light path and align with baffle position, while light path be positioned at blood sample ullage to be detected; 3rd light wave generator 8c and the 3rd Lightwave receiver 9c subtend are arranged on detection cup both sides and light path is positioned at below baffle plate, and light path is through blood sample to be detected simultaneously.
After adding clotting reagent, as blood sample coagulation and adhere to, then blood clot sticks to the bottom regional area detecting cup, and rotate with detection cup and move, when blood clot (containing wherein can not through ultrared particle 4) rotates to the light path position between Infrared irradiation-detecting device, detecting device can detect lightwave signal change 20% and more than, or lightwave signal change difference is greater than 100mV, if the light signal now received is B, the change in optical signal that then the second Lightwave receiver 9b receives is the regularity change being become 4300-B-4300-B-4300 by initial 4300mv, the change in optical signal that 3rd Lightwave receiver 9c receives light wave generator 8c transmitting is the regularity change being become 0-B-0 by initial 0mv,
As blood sample coagulation does not adhere to, along with detection cup rotates, due to the effect of lagging, blood clot gets back to bottom after being taken to the second detection position by lagging, but can not arrive and detect above cup, therefore the change in optical signal that the second Lightwave receiver 9b receives is that the regularity becoming 4300-B-4300 by initial 4300mv changes, and the initial 0mv of the optical signals that the 3rd Lightwave receiver 9c receives becomes the regularity change of 0-B-0;
Do not adhere to as blood sample does not solidify, blood sample is in all the time and detects bottom cup, then the light signal that the second Lightwave receiver 9b receives is always 4300mv, and the light signal that the 3rd Lightwave receiver 9c receives is always 0mv.
Table 4 detecting device to be separately positioned on below baffle position and baffle plate and to detect the result (light signal unit: mv) that cup rotates a circle
| Receiver (9b) | Receiver (9c) | Testing result | |
| Light signal 1 | 4300-B-4300-B-4300 | 0-B-0 | Solidify adhesion |
| Light signal 2 | 4300-B-4300 | 0-B-0 | Solidify and do not adhere to |
| Light signal 3 | 4300 | 0 | Do not solidify and do not adhere to |
Embodiment 3
Composition graphs 9, the difference of the present embodiment and embodiment 1 is, above baffle plate and below baffle plate, both sides are respectively provided with one group of light wave generator and Lightwave receiver, namely the first light wave generator 8a and the first Lightwave receiver 9a subtend are arranged on and detect the cup left and right sides and light path is positioned at above baffle plate, and light path is positioned at blood sample ullage to be detected simultaneously; 3rd light wave generator 8c and the 3rd Lightwave receiver 9c subtend are arranged on detection cup both sides and light path is positioned at below baffle plate, and light path is through blood sample to be detected simultaneously.
After adding clotting reagent, as blood sample coagulation adheres to, then sludged blood sticks to the bottom regional area detecting cup, just with the rotary motion of detection cup after blood clot is formed, when blood clot (containing wherein can not through ultrared particle) rotates to the light path position between Infrared irradiation-detecting device, detecting device can detect lightwave signal change 20% and more than, or lightwave signal change difference is greater than 100mV, if the light signal now received is B, then the first Lightwave receiver 9a receives the regularity that the initial 4300mv of optical signals that light wave generator 8a launches becomes in 4300-B-4300 in detecting cup and rotating a circle and changes, 3rd Lightwave receiver 9c receives the regularity that the initial 0mv of optical signals that the 3rd light wave generator 8c launches becomes in 0-B-0 in detecting cup and rotating a circle to be changed,
As blood sample coagulation does not adhere to, along with detection cup rotates, due to the effect of lagging, blood clot gets back to bottom after being taken to the second detection position by lagging, but can not arrive and detect above cup, therefore the light signal that the first Lightwave receiver 9a receives is always 4300mv, and the regularity that the signal that the 3rd Lightwave receiver 9c detects becomes 0-B-0 by initial 0mv changes;
Do not adhere to as blood sample does not solidify, blood sample is in all the time and detects bottom cup, then the light signal that the first Lightwave receiver 9a receives is always 4300mv, and the light signal that the 3rd Lightwave receiver 9c receives is always 0mv.
Table 5 detecting device to be separately positioned on above baffle plate and to detect the result (light signal unit: mv) that cup rotates a circle below baffle plate
| Receiver (9a) | Receiver (9c) | Testing result | |
| Light signal 1 | 4300-B-4300 | 0-B-0 | Solidify adhesion |
| Light signal 2 | 4300 | 0-B-0 | Solidify and do not adhere to |
| Light signal 3 | 4300 | 0 | Do not solidify and do not adhere to |
Above-described embodiment is only optimal way of the present utility model, and for those skilled in the art, under the prerequisite not departing from the utility model principle, can also make some improvement, these improvement should be considered as protection domain of the present utility model.
Claims (6)
1. whole blood blood coagulation and an adhesion function detector, is characterized in that: the whirligig, light wave generator, Lightwave receiver and the light tight housing that comprise the detection cup for placing blood sample to be detected, are connected with detection cup;
Described detection cup inner bottom is provided with printing opacity lagging, leaves space between lagging and detection cup inwall;
Described whirligig rotates around its central axis for driving detection cup;
Described light wave generator and Lightwave receiver subtend are arranged on and detect cup both sides and be at least two groups, for detecting the light wave intensity through detecting cup;
Detect cup, light wave generator and Lightwave receiver to be all arranged in light tight housing.
2. whole blood blood coagulation according to claim 1 and adhesion function detector, is characterized in that: described light wave generator and Lightwave receiver quantity are two groups, is separately positioned on any two positions in primary importance, the second place and the 3rd position;
Described primary importance is that light wave generator and Lightwave receiver subtend are arranged on the left and right sides of detecting cup and higher than the position of blood sample liquid level to be detected, light wave is positioned at above lagging simultaneously;
The described second place is that light wave generator and Lightwave receiver subtend are arranged on the left and right sides of detecting cup and higher than the position of blood sample liquid level to be detected, light wave is through lagging simultaneously;
Described 3rd position is that light wave generator and Lightwave receiver subtend are arranged on the left and right sides of detecting cup and lower than the position of blood sample liquid level to be detected, light wave is positioned at below lagging simultaneously.
3. whole blood blood coagulation according to claim 2 and adhesion function detector, is characterized in that: described detector also comprises the detecting position with heated at constant temperature function, and described detecting position parcel is arranged on the outside detecting cup.
4. the whole blood blood coagulation according to claim 1-3 any one and adhesion function detector, is characterized in that: described detector also comprises timer, for recording the blood sample coagulation time to be detected.
5. the whole blood blood coagulation according to claim 1-3 any one and adhesion function detector, is characterized in that: described detection cup axis becomes 3-80 ° of angle with surface level, and become rotating along its central axis of constant speed when detecting.
6. the whole blood blood coagulation according to claim 1-3 any one and adhesion function detector, is characterized in that: described light wave generator is the light wave generator of more than 700nm wavelength.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520514713.XU CN204789228U (en) | 2015-07-15 | 2015-07-15 | Whole blood blood coagulation and adhesion work can detectors |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520514713.XU CN204789228U (en) | 2015-07-15 | 2015-07-15 | Whole blood blood coagulation and adhesion work can detectors |
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| CN204789228U true CN204789228U (en) | 2015-11-18 |
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| CN201520514713.XU Withdrawn - After Issue CN204789228U (en) | 2015-07-15 | 2015-07-15 | Whole blood blood coagulation and adhesion work can detectors |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104977229A (en) * | 2015-07-15 | 2015-10-14 | 江苏英诺华医疗技术有限公司 | Whole-blood blood coagulation and conglutination function detector and detection method |
-
2015
- 2015-07-15 CN CN201520514713.XU patent/CN204789228U/en not_active Withdrawn - After Issue
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104977229A (en) * | 2015-07-15 | 2015-10-14 | 江苏英诺华医疗技术有限公司 | Whole-blood blood coagulation and conglutination function detector and detection method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20151118 Effective date of abandoning: 20171117 |
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| AV01 | Patent right actively abandoned |