CN104977229A - Whole-blood blood coagulation and conglutination function detector and detection method - Google Patents
Whole-blood blood coagulation and conglutination function detector and detection method Download PDFInfo
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- CN104977229A CN104977229A CN201510417138.6A CN201510417138A CN104977229A CN 104977229 A CN104977229 A CN 104977229A CN 201510417138 A CN201510417138 A CN 201510417138A CN 104977229 A CN104977229 A CN 104977229A
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Abstract
The invention discloses a whole-blood blood coagulation and conglutination function detector and a detection method. The detector comprises a detection cup for containing a blood sample to be detected, and a rotary device, optical wave generators, optical wave receivers and a non-light-transmitting shell which are connected with the detection cup, wherein a light-transmitting transverse baffle is arranged at the bottom end in the detection cup; and a gap is formed between the transverse baffle and the inner wall of the detection cup. The detection method comprises the following steps: carrying out continuous detection on a blood sample in the detection cup through at least two groups of the optical wave generators and the optical wave receivers, which are oppositely arranged on the two sides of the detection cup; and judging the status of the blood sample by detecting the optical wave intensity passing through the detection cup. According to the whole-blood blood coagulation and conglutination function detector, whole-blood blood coagulation and conglutination functions can be detected on one instrument so that an operation procedure is simplified and the detection time is shortened.
Description
Technical field
The invention belongs to clinical medical inspection field, particularly a kind of whole blood blood coagulation and adhesion function detector and detection method.
Background technology
Blood coagulation function, adhesion function are the critical functions of blood, and these functions change and there is close relationship with thrombus, hemorrhage and some other blood disease.Existing coagulo meter is by turbidimetry, and the principle utilizing blood turbidity in coagulation process to raise suddenly designs, and only evaluates blood coagulation function, does not have the detection of blood adhesion function.Existing platelet adhesiveness test carries out external test to hematoblastic adhesiveness, blood platelet has and adheres to non-physiologic surface of contact as the critical function on the materials such as glass, the collagenous fibres exposed after vascular wall breaks have special compatibility to blood platelet, make platelet adhesion reaction sex change, Adherence of Platelet lowers, and easily occurs hemorrhage; Adherence of Platelet increases, and thrombus is easy to be formed.
Because blood coagulation function and adhesion function have detected respectively on coagulo meter and platelet adhesion reaction instrument, because this increasing the consumption of blood sample, add the workload of operator, work efficiency is lower.
Summary of the invention
The object of invention is to provide a kind of whole blood blood coagulation and adhesion function detector and detection method.
The technical scheme realizing the object of the invention is:
A kind of whole blood blood coagulation and adhesion function detector, the whirligig, light wave generator, Lightwave receiver and the light tight housing that comprise the detection cup for placing blood sample to be detected, are connected with detection cup;
Described detection cup inner bottom is provided with printing opacity lagging, leaves space between lagging and detection cup inwall;
Described whirligig rotates around its central axis for driving detection cup;
Described light wave generator and Lightwave receiver subtend are arranged on and detect cup both sides and be at least two groups, for detecting the light wave intensity through detecting cup;
Detect cup, light wave generator and Lightwave receiver to be all arranged in light tight housing.
A kind of whole blood blood coagulation and adhesion function detection method, comprise the following steps:
Step 1, adds whole blood to be detected in detection cup;
Step 2, becomes the angle of 3-80 ° with surface level by this detection cup, detect the central shaft rotation of cup constant speed around self;
Step 3, adds clotting reagent, and light wave generator and Lightwave receiver carry out continuous detecting to blood sample in detection cup, by detecting through judging blood sample state through the light wave intensity detecting cup, specific as follows:
If when the light signal that light wave generator sends is through empty detection cup, the light signal strength that Lightwave receiver receives is A; If light signal strength A reduce more than 20% or reduce 100mv and above time the light signal strength that receives be B; If the light signal of the light wave generator light signal strength that Lightwave receiver receives after blood sample to be measured is C;
(1) if the light signal strength that the Lightwave receiver of primary importance receives is the change of A-B-A regularity in detection cup rotates a circle, simultaneously the light signal strength that receives of the Lightwave receiver of the second place is regular in A-B-A-B-A in detection cup rotates a circle changes, then blood sample coagulation to be measured and adhering to;
If the light signal strength that the Lightwave receiver of the second place receives is the regularity change in A-B-A-B-A in detection cup rotates a circle, simultaneously the light signal strength that receives of the Lightwave receiver of the 3rd position is regular in C-B-C in detection cup rotates a circle changes, then blood sample coagulation to be measured and adhering to;
If the light signal strength that the Lightwave receiver of primary importance receives is the regularity change in A-B-A in detection cup rotates a circle, simultaneously the light signal strength that receives of the Lightwave receiver of the 3rd position is regular in C-B-C in detection cup rotates a circle changes, then blood sample coagulation to be measured and adhering to;
(2) if the light signal strength that receives of the Lightwave receiver of primary importance is constant, and the light signal strength that receives of the Lightwave receiver of the second place is regular in A-B-A in detection cup rotates a circle changes, then blood sample coagulation to be measured but do not adhere to;
If the light signal strength that the Lightwave receiver of the second place receives is the regularity change in A-B-A in detection cup rotates a circle, and the light signal strength that receives of the Lightwave receiver of the 3rd position is regular in C-B-C in detection cup rotates a circle changes, then blood sample coagulation to be measured but do not adhere to;
If the light signal strength that the Lightwave receiver of primary importance receives is constant, and the light signal strength that receives of the Lightwave receiver of the 3rd position is regular in C-B-C in detection cup rotates a circle changes, then blood sample coagulation to be measured but do not adhere to;
(3) if the light signal strength that in primary importance, the second place and the 3rd position, the Lightwave receiver of any two positions receives is constant all the time, then blood sample to be measured does not solidify and does not adhere to yet.
Compared with prior art, remarkable result of the present invention is: (1) present invention achieves blood coagulation function and adhesion function completes detection on an instrument, original respective independently testing process is effectively integrated, simplifies procedures, save detection time; (2) the present invention only needs a blood sample single injected sampling just can complete blood coagulation function and adhesion function detection, reduces the restriction of sample collection difficulty; (3) the analyser structure of the present invention's employing is simple, detects the waste liquid produced few, is conducive to environmental protection.
Accompanying drawing explanation
Detecting instrument texture edge schematic diagram when Fig. 1 is initial time blood sample ot-yet-hardened.
Fig. 2 is that blood sample has solidified and adhered to, and rotates extremely higher than detecting instrument texture edge schematic diagram during former blood sample surface level with detection cup.
Fig. 3 be two groups of light wave generators and receiver to be separately positioned on above baffle plate and baffle position time detecting instrument structural profile schematic diagram.
Fig. 4 is that conventional plasma detection method and detection method of the present invention detect TT results relevance analysis chart.
Fig. 5 is that conventional plasma detection method and detection method of the present invention detect PT results relevance analysis chart.
Fig. 6 is that detection method of the present invention (X) detects same sample TT result consistency analysis figure with blood plasma method (Y).
Fig. 7 is that detection method of the present invention (X) detects same sample PT result consistency analysis figure with blood plasma method (Y).
Fig. 8 be two groups of light wave generators and receiver to be separately positioned on below baffle plate and baffle position time detecting instrument structural profile schematic diagram.
Fig. 9 be two groups of light wave generators and receiver to be separately positioned on above baffle plate and below baffle plate time detecting instrument structural profile schematic diagram.
Embodiment
Composition graphs 1, a kind of whole blood blood coagulation and adhesion function detector, the whirligig, light wave generator, Lightwave receiver and the light tight housing that comprise the detection cup for placing blood sample to be detected, are connected with detection cup;
Described detection cup inner bottom is provided with printing opacity lagging, and lagging is arranged with detection cup bottom vertical, and leave space between lagging both sides and detection cup inwall, space is not more than 1mm;
Described whirligig rotates around its central axis for driving detection cup;
Described light wave generator and Lightwave receiver subtend are arranged on and detect cup both sides and be at least two groups, for detecting the light wave intensity through detecting cup;
Detect cup, light wave generator and Lightwave receiver to be all arranged in light tight housing.
Further, when light wave generator and Lightwave receiver quantity are two groups, primary importance and the second place can be arranged on, or be arranged on the second place and the 3rd position, primary importance and the 3rd position can be arranged on.
Described primary importance is that light wave generator and Lightwave receiver subtend are arranged on the left and right sides of detecting cup and higher than the position of blood sample liquid level to be detected, light wave is positioned at above lagging simultaneously;
The described second place is that light wave generator and Lightwave receiver subtend are arranged on the left and right sides of detecting cup and higher than the position of blood sample liquid level to be detected, light wave is through lagging simultaneously;
Described 3rd position is that light wave generator and Lightwave receiver subtend are arranged on the left and right sides of detecting cup and lower than the position of blood sample liquid level to be detected, light wave is positioned at below lagging simultaneously.
Further, described detector also comprises the detecting position with heated at constant temperature function, and described detecting position parcel is arranged on the outside detecting cup.
Described detector also comprises timer, for recording the blood sample coagulation time to be detected.
Described detection cup axis becomes 3-80 ° of angle with surface level, and becomes rotating along its central axis of constant speed when detecting.
Described light wave generator is the light wave generator of more than 700nm wavelength.
A kind of whole blood blood coagulation and adhesion function detection method, comprise the following steps:
Step 1, adds whole blood to be detected in detection cup;
Step 2, becomes the angle of 3-80 ° with surface level by this detection cup, detect the central shaft rotation of cup constant speed around self;
Step 3, adds clotting reagent, and light wave generator and Lightwave receiver carry out continuous detecting to blood sample in detection cup, by detecting through judging blood sample state through the light wave intensity detecting cup, specific as follows:
If when the light signal that light wave generator sends is through empty detection cup, the light signal strength that Lightwave receiver receives is A; If light signal strength A reduce more than 20% or reduce 100mv and above time the light signal strength that receives be B; If the scope that the light signal of the light wave generator light signal strength that Lightwave receiver receives after blood sample to be measured is C, C is 0-10mv;
(1) if the light signal that the Lightwave receiver of primary importance receives is the change of A-B-A regularity in detection cup rotates a circle, simultaneously the light signal that receives of the Lightwave receiver of the second place is regular in A-B-A-B-A in detection cup rotates a circle changes, then blood sample coagulation to be measured and adhering to;
If the light signal that the Lightwave receiver of the second place receives is the regularity change in A-B-A-B-A in detection cup rotates a circle, simultaneously the light signal that receives of the Lightwave receiver of the 3rd position is regular in C-B-C in detection cup rotates a circle changes, then blood sample coagulation to be measured and adhering to;
If the light signal that the Lightwave receiver of primary importance receives is the regularity change in A-B-A in detection cup rotates a circle, simultaneously the light signal that receives of the Lightwave receiver of the 3rd position is regular in C-B-C in detection cup rotates a circle changes, then blood sample coagulation to be measured and adhering to;
(2) if the light signal that receives of the Lightwave receiver of primary importance is constant, and the light signal that receives of the Lightwave receiver of the second place is regular in A-B-A in detection cup rotates a circle changes, then blood sample coagulation to be measured but do not adhere to;
If the light signal that the Lightwave receiver of the second place receives is the regularity change in A-B-A in detection cup rotates a circle, and the light signal that receives of the Lightwave receiver of the 3rd position is regular in C-B-C in detection cup rotates a circle changes, then blood sample coagulation to be measured but do not adhere to;
If the light signal that the Lightwave receiver of primary importance receives is constant, and the light signal that receives of the Lightwave receiver of the 3rd position is regular in C-B-C in detection cup rotates a circle changes, then blood sample coagulation to be measured but do not adhere to;
(3) if the light signal that in primary importance, the second place and the 3rd position, the Lightwave receiver of any two positions receives is constant all the time, then blood sample to be measured does not solidify and does not adhere to yet.
Namely judge that blood sample to be measured does not solidify also do not adhere to if blood sample did not adhere to more than 180 seconds from adding the reagent moment, solidify change.
Further, step 1 is also included in detect in cup and adds not through the granulated solid material of more than 700nm wavelength light, and its maximum gauge or length are less than 10mm.
Whole blood and dilution are carried out diluting rear detection during detection, wherein the dilution ratio of dilution and blood sample is less than 2:1; Above-mentioned dilution is physiological saline or isotonic phosphate buffer liquid.
Below in conjunction with specific embodiment, the present invention will be further described.
Embodiment 1
Composition graphs 1, Fig. 3, a kind of whole blood blood coagulation and adhesion function detector, comprise whirligig 1, coupling arrangement 2, pick-up unit;
Described whirligig 1 is connected with pick-up unit by coupling arrangement 2, carries out unidirectional constant speed rotary or reciprocating rotation at the uniform velocity for driving detection cup around its central axis; Described pick-up unit comprises and detects cup 6, detecting position 3, first light wave generator 8a with heating function, the first Lightwave receiver 9a, the second light wave generator 8b and the second Lightwave receiver 9b; Detect bottom cup and be provided with printing opacity lagging 10, lagging is arranged with detection cup bottom vertical, and leave space between lagging both sides and detection cup inwall, space is not more than 1mm, and detecting position 3 parcel is arranged on and detects cup periphery; First light wave generator 8a and the first Lightwave receiver 9a subtend is arranged on the left and right sides of detecting device and light path is positioned at above baffle plate, second light wave generator 8b and the second Lightwave receiver 9b subtend are arranged on the left and right sides of detecting device and light path is alignd with baffle position, wherein, the first light wave generator 8a, the first Lightwave receiver 9a, the second light wave generator 8b and the second Lightwave receiver 9b are all higher than whole blood blood sample 5 liquid level.
Described detection cup is under whirligig 1 drives, and by coupling arrangement 2, detect cup 6 with central shaft x-x ' for axle center rotates, z-z ' is transverse axis, and x-x ' axle becomes 3-80 degree angle with z-z ' axle; The invariablenes turning speed of described whirligig is set to often revolve the 2s that turns around.
Check point 7 be arranged on detect cup both sides, detecting position is provided with through hole in the position of check point 7, be convenient to infrared light through.Light wave generator and Lightwave receiver are separately positioned on the both sides of check point 7, only have detect cup or inside only have a thin layer blood film time, infrared ray can by detecting cup and blood thin layer, therefore detecting device can obtain stronger detection signal, maximumly reaches 4300mV.During detection, detecting position and detection cup should be the states isolated with extraneous light.Add the clotting reagent time to the time length that blood clotting is formed by record, the coagulation function namely can evaluating tested blood sample is strong and weak.
The present embodiment detects cup axis and becomes 25 degree of angles with surface level, and both sides are respectively provided with one group of detecting device, composition graphs 3 above detection cup baffle plate both sides and baffle plate, and the detection method of the present embodiment is:
Step 1, gather patient whole blood sample with citrate anticoagulation pipe, before detecting, quantitative citrate anticoagulation whole blood (0.2ml) is added to and detect in cup, and in blood sample, add 0.2ml physiological saline sample 1:1 is diluted, improve the mobility of sample blood, improve detection sensitivity.
Step 2, a detecting position tilted is placed in by detecting cup, this detecting position axis becomes 25 degree of angles with surface level, and has heated constant temperature function and drive detection cup along the function of cup central shaft rotary motion, and during detection, detecting position and detection cup should be the states isolated with extraneous light.
Step 3, detect time wave producer to continue to send infrared light, although now detect cup to continue to rotate with its central axis, but because blood sample is also in liquid state, can not rotate with detection cup, now at the infrared detector continuous reception infrared light detecting the setting of cup offside relevant position, peak signal is 4300mV.
Add clotting reagent, as blood sample coagulation adheres to, then blood clot sticks to the bottom regional area detecting cup, and with the rotary motion of detection cup, as shown in Figure 2, when blood clot rotates to the light path position between infrared light and detecting device, detecting device can detect lightwave signal change 20% and more than, or lightwave signal change difference is greater than 100mV, if the light signal received is B, then after this detection cup rotate a circle interior first Lightwave receiver 9a receive light signal become 4300-B-4300 regularity change, second Lightwave receiver 9b receives light signal and becomes the change of 4300-B-4300-B-4300 regularity,
As blood sample coagulation does not adhere to, along with detection cup rotates, due to the effect of lagging, blood clot gets back to bottom after being taken to the second detection position by lagging, but can not arrive and detect above cup, therefore the light signal that the first Lightwave receiver 9a receives is always 4300mv, changes detecting the cup regularity that signal that interior Lightwave receiver 9b detects becomes 4300-B-4300 by initial 4300mv that rotates a circle;
Do not adhere to as blood sample does not solidify, blood sample is in all the time and detects bottom cup, then Lightwave receiver 9a and 9b receives the light signal that light wave generator 8a and 8b launch and be always 4300mv.
Record adds the time of clotting reagent and the time of blood clotting formation, detects the blood sample clotting time thus, thus judges the coagulation function of blood sample, and change formation time is shorter then illustrates that corresponding function is stronger.
Table 1 detecting device to be separately positioned on above baffle plate and baffle position place and detect the result (light signal unit: mv) that cup rotates a circle after certain hour
| Receiver (9a) | Receiver (9b) | Testing result | |
| Light signal 1 | 4300-B-4300 | 4300-B-4300-B-4300 | Solidify adhesion |
| Light signal 2 | 4300 | 4300-B-4300 | Solidify and do not adhere to |
| Light signal 3 | 4300 | 4300 | Do not solidify and do not adhere to |
Classical Clinical significant of coagulation function detection method and the inventive method is adopted to detect same sample results comparison test.
The same sample of table 2 compares with the inventive method testing result (unit: second) by classical blood plasma detection method respectively
Classical blood plasma detection method and the inventive method is adopted to detect TT (thrombin time), PT (prothrombin time) respectively, classical blood plasma detection method is: the citrate anticoagulation whole blood of collection is gone out blood plasma through centrifuging, add in detection cup by being separated the blood plasma obtained according to detection requirement, constant-temperature incubation, then add certain TT or PT reagent, the sample clotting time is determined in the change adding transmitted light or scattered light in rear reaction system by detecting reagent.Two kinds of methods detect the repeated CV value of same sample, and TT (thrombin time) is respectively: classical blood plasma detection method 1.23%, the inventive method 1.10%; PT (prothrombin time) is respectively: classical blood plasma detection method 1.55%, the inventive method 1.51%.Two kinds of methods detect the result mean value of same sample, and TT is respectively: classical blood plasma method 14.265 seconds, the inventive method 14.245 seconds; PT is respectively: classical blood plasma method 12.295 seconds, the inventive method 12.30 seconds.
Result illustrates that the inventive method detects the TT (thrombin time) that obtains and PT (prothrombin time) and all has good repeatability and consistance.
Classical Clinical significant of coagulation function detection method and the inventive method is adopted to detect different sample results comparison test.
Table 3 different specimens compares with the inventive method testing result (unit: second) by classical blood plasma detection method respectively
1. correlation analysis
Fig. 4 and Fig. 5, two kinds of distinct methods testing result correlativitys: RTT=0.974 >=0.95, RPT=0.970 >=0.95, illustrates that two kinds of method testing result correlativitys are good.
2. consistency analysis (Bland-altman analysis)
As shown in Figure 6, compared with the inventive method (X) detects TT (Y) with classical blood plasma method, the point of 100% is all in 95% consistance interval, and two methods and results consistance are good.
As shown in Figure 7, compared with the inventive method (X) detects PT (Y) with classical blood plasma method, the point of 100% is all in 95% consistance interval, and two methods and results consistance are good.
Conclusion two kinds of methods measure same sample respectively, its testing result correlativity and consistance good.Two kinds of detection method results are equivalent, can mutually replace.
Embodiment 2
Composition graphs 8, the present embodiment and embodiment 1 be distinguished as two groups of light wave generators and receiver is separately positioned on both sides below baffle plate both sides and baffle plate, namely the second light wave generator 8b and the second Lightwave receiver 9b subtend are arranged on and detect the cup left and right sides and light path and align with baffle position, while light path be positioned at blood sample ullage to be detected; 3rd light wave generator 8c and the 3rd Lightwave receiver 9c subtend are arranged on detection cup both sides and light path is positioned at below baffle plate, and light path is through blood sample to be detected simultaneously.
After adding clotting reagent, as blood sample coagulation and adhere to, then blood clot sticks to the bottom regional area detecting cup, and rotate with detection cup and move, when blood clot (containing wherein can not through ultrared particle 4) rotates to the light path position between Infrared irradiation-detecting device, detecting device can detect lightwave signal change 20% and more than, or lightwave signal change difference is greater than 100mV, if the light signal now received is B, the change in optical signal that then the second Lightwave receiver 9b receives is the regularity change being become 4300-B-4300-B-4300 by initial 4300mv, the change in optical signal that 3rd Lightwave receiver 9c receives light wave generator 8c transmitting is the regularity change being become 0-B-0 by initial 0mv,
As blood sample coagulation does not adhere to, along with detection cup rotates, due to the effect of lagging, blood clot gets back to bottom after being taken to the second detection position by lagging, but can not arrive and detect above cup, therefore the change in optical signal that the second Lightwave receiver 9b receives is that the regularity becoming 4300-B-4300 by initial 4300mv changes, and the initial 0mv of the optical signals that the 3rd Lightwave receiver 9c receives becomes the regularity change of 0-B-0;
Do not adhere to as blood sample does not solidify, blood sample is in all the time and detects bottom cup, then the light signal that the second Lightwave receiver 9b receives is always 4300mv, and the light signal that the 3rd Lightwave receiver 9c receives is always 0mv.
Table 4 detecting device to be separately positioned on below baffle position and baffle plate and to detect the result (light signal unit: mv) that cup rotates a circle
| Receiver (9b) | Receiver (9c) | Testing result | |
| Light signal 1 | 4300-B-4300-B-4300 | 0-B-0 | Solidify adhesion |
| Light signal 2 | 4300-B-4300 | 0-B-0 | Solidify and do not adhere to |
| Light signal 3 | 4300 | 0 | Do not solidify and do not adhere to |
Embodiment 3
Composition graphs 9, the difference of the present embodiment and embodiment 1 is, above baffle plate and below baffle plate, both sides are respectively provided with one group of light wave generator and Lightwave receiver, namely the first light wave generator 8a and the first Lightwave receiver 9a subtend are arranged on and detect the cup left and right sides and light path is positioned at above baffle plate, and light path is positioned at blood sample ullage to be detected simultaneously; 3rd light wave generator 8c and the 3rd Lightwave receiver 9c subtend are arranged on detection cup both sides and light path is positioned at below baffle plate, and light path is through blood sample to be detected simultaneously.
After adding clotting reagent, as blood sample coagulation adheres to, then sludged blood sticks to the bottom regional area detecting cup, just with the rotary motion of detection cup after blood clot is formed, when blood clot (containing wherein can not through ultrared particle) rotates to the light path position between Infrared irradiation-detecting device, detecting device can detect lightwave signal change 20% and more than, or lightwave signal change difference is greater than 100mV, if the light signal now received is B, then the first Lightwave receiver 9a receives the regularity that the initial 4300mv of optical signals that light wave generator 8a launches becomes in 4300-B-4300 in detecting cup and rotating a circle and changes, 3rd Lightwave receiver 9c receives the regularity that the initial 0mv of optical signals that the 3rd light wave generator 8c launches becomes in 0-B-0 in detecting cup and rotating a circle to be changed,
As blood sample coagulation does not adhere to, along with detection cup rotates, due to the effect of lagging, blood clot gets back to bottom after being taken to the second detection position by lagging, but can not arrive and detect above cup, therefore the light signal that the first Lightwave receiver 9a receives is always 4300mv, and the regularity that the signal that the 3rd Lightwave receiver 9c detects becomes 0-B-0 by initial 0mv changes;
Do not adhere to as blood sample does not solidify, blood sample is in all the time and detects bottom cup, then the light signal that the first Lightwave receiver 9a receives is always 4300mv, and the light signal that the 3rd Lightwave receiver 9c receives is always 0-10mv.
Table 5 detecting device to be separately positioned on above baffle plate and to detect the result (light signal unit: mv) that cup rotates a circle below baffle plate
| Receiver (9a) | Receiver (9c) | Testing result | |
| Light signal 1 | 4300-B-4300 | 0-B-0 | Solidify adhesion |
| Light signal 2 | 4300 | 0-B-0 | Solidify and do not adhere to |
| Light signal 3 | 4300 | 0 | Do not solidify and do not adhere to |
Above-described embodiment is only optimal way of the present invention, and for those skilled in the art, under the premise without departing from the principles of the invention, can also make some improvement, these improvement should be considered as protection scope of the present invention.
Claims (10)
1. whole blood blood coagulation and an adhesion function detector, is characterized in that: the whirligig, light wave generator, Lightwave receiver and the light tight housing that comprise the detection cup for placing blood sample to be detected, are connected with detection cup;
Described detection cup inner bottom is provided with printing opacity lagging, leaves space between lagging and detection cup inwall;
Described whirligig rotates around its central axis for driving detection cup;
Described light wave generator and Lightwave receiver subtend are arranged on and detect cup both sides and be at least two groups, for detecting the light wave intensity through detecting cup;
Detect cup, light wave generator and Lightwave receiver to be all arranged in light tight housing.
2. whole blood blood coagulation according to claim 1 and adhesion function detector, is characterized in that: described light wave generator and Lightwave receiver quantity are two groups, is separately positioned on any two positions in primary importance, the second place and the 3rd position;
Described primary importance is that light wave generator and Lightwave receiver subtend are arranged on the left and right sides of detecting cup and higher than the position of blood sample liquid level to be detected, light wave is positioned at above lagging simultaneously;
The described second place is that light wave generator and Lightwave receiver subtend are arranged on the left and right sides of detecting cup and higher than the position of blood sample liquid level to be detected, light wave is through lagging simultaneously;
Described 3rd position is that light wave generator and Lightwave receiver subtend are arranged on the left and right sides of detecting cup and lower than the position of blood sample liquid level to be detected, light wave is positioned at below lagging simultaneously.
3. whole blood blood coagulation according to claim 2 and adhesion function detector, is characterized in that: described detector also comprises the detecting position with heated at constant temperature function, and described detecting position parcel is arranged on the outside detecting cup.
4. the whole blood blood coagulation according to claim 1-3 any one and adhesion function detector, is characterized in that: described detector also comprises timer, for recording the blood sample coagulation time to be detected.
5. the whole blood blood coagulation according to claim 1-3 any one and adhesion function detector, is characterized in that: described detection cup axis becomes 3-80 ° of angle with surface level, and when detecting the rotating around its central axis of constant speed.
6. the whole blood blood coagulation according to claim 1-3 any one and adhesion function detector, is characterized in that: described light wave generator is the light wave generator of more than 700nm wavelength.
7., based on whole blood blood coagulation and the adhesion function detection method of whole blood blood coagulation and adhesion function detector described in claim 2, it is characterized in that, comprise the following steps:
Step 1, adds whole blood to be detected in detection cup;
Step 2, becomes the angle of 3-80 ° with surface level by this detection cup, detect the central shaft rotation of cup constant speed around self;
Step 3, adds clotting reagent, and light wave generator and Lightwave receiver carry out continuous detecting to blood sample in detection cup, judge blood sample state by the light wave intensity detected through detecting cup, specific as follows:
If when the light signal that light wave generator sends is through empty detection cup, the light signal strength that Lightwave receiver receives is A; If light signal strength A reduce more than 20% or reduce 100mv and above time the light signal strength that receives be B; If the light signal of the light wave generator light signal strength that Lightwave receiver receives after blood sample to be measured is C;
(1) if the light signal strength that the Lightwave receiver of primary importance receives is the change of A-B-A regularity in detection cup rotates a circle, simultaneously the light signal strength that receives of the Lightwave receiver of the second place is regular in A-B-A-B-A in detection cup rotates a circle changes, then blood sample coagulation to be measured and adhering to;
If the light signal strength that the Lightwave receiver of the second place receives is the regularity change in A-B-A-B-A in detection cup rotates a circle, simultaneously the light signal strength that receives of the Lightwave receiver of the 3rd position is regular in C-B-C in detection cup rotates a circle changes, then blood sample coagulation to be measured and adhering to;
If the light signal strength that the Lightwave receiver of primary importance receives is the regularity change in A-B-A in detection cup rotates a circle, simultaneously the light signal strength that receives of the Lightwave receiver of the 3rd position is regular in C-B-C in detection cup rotates a circle changes, then blood sample coagulation to be measured and adhering to;
(2) if the light signal strength that receives of the Lightwave receiver of primary importance is constant, and the light signal strength that receives of the Lightwave receiver of the second place is regular in A-B-A in detection cup rotates a circle changes, then blood sample coagulation to be measured but do not adhere to;
If the light signal strength that the Lightwave receiver of the second place receives is the regularity change in A-B-A in detection cup rotates a circle, and the light signal strength that receives of the Lightwave receiver of the 3rd position is regular in C-B-C in detection cup rotates a circle changes, then blood sample coagulation to be measured but do not adhere to;
If the light signal strength that the Lightwave receiver of primary importance receives is constant, and the light signal strength that receives of the Lightwave receiver of the 3rd position is regular in C-B-C in detection cup rotates a circle changes, then blood sample coagulation to be measured but do not adhere to;
(3) if the light signal strength that in primary importance, the second place and the 3rd position, the Lightwave receiver of any two positions receives is constant all the time, then blood sample to be measured does not solidify and does not adhere to yet.
8. whole blood blood coagulation according to claim 7 and adhesion function detector, is characterized in that: step 1 is also included in detect in cup and adds not through the granulated solid material of more than 700nm wavelength light, and its maximum gauge or length are less than 10mm.
9. whole blood blood coagulation according to claim 7 and adhesion function detection method, is characterized in that: whole blood and dilution are carried out diluting rear detection during detection, wherein the dilution ratio of dilution and blood sample is less than 2:1.
10. whole blood blood coagulation according to claim 7 and adhesion function detection method, is characterized in that: if blood sample adhering to more than 180 seconds from adding the reagent moment, solidifying change and namely judge that blood sample to be measured does not solidify and also do not adhere to.
Priority Applications (1)
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| CN109580924A (en) * | 2017-09-29 | 2019-04-05 | 重庆南方数控设备有限责任公司 | The frequency domain parameter analysis method and system of thrombelastogram process of setting |
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| CN104977229B (en) | 2017-11-17 |
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