CN204499260U - A kind of novel sperm cryopreservation device being applicable to severe oligospermia - Google Patents
A kind of novel sperm cryopreservation device being applicable to severe oligospermia Download PDFInfo
- Publication number
- CN204499260U CN204499260U CN201520155950.1U CN201520155950U CN204499260U CN 204499260 U CN204499260 U CN 204499260U CN 201520155950 U CN201520155950 U CN 201520155950U CN 204499260 U CN204499260 U CN 204499260U
- Authority
- CN
- China
- Prior art keywords
- tampon
- groove
- freezing
- applicable
- freezing straw
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Abstract
A kind of novel sperm cryopreservation device being applicable to severe oligospermia, comprise hand lever, support, groove, freezing straw, tampon, adhesive label's paper, described hand lever is located on two symmetrical supports, two symmetrical supports are equipped with groove, freezing straw two ends are arranged in groove symmetrical on two supports, freezing straw one end is provided with tampon, tampon peripheral hardware toughness label paper.The amount of liquid loaded in the every only freezing straw of the utility model is few, and time stifling, liquid is caught a cold evenly, and refrigerating effect is good, easily can find sperm during recovery, and once can fumigate many parts of semen samples, increases work efficiency.
Description
Technical field
The utility model relates to a kind of sperm cryopreservation device being applicable to severe oligospermia.
Background technology
Severe oligospermia refers to sperm concentration < 1 × 10
6/ mL, the sole mode that this type of patient obtains offspring is injection (ICSI) in monosperm ooecium slurry, certain fluctuation is had owing to getting its sperm quality of essence at every turn, probably get ovum its spouse and can not find the capable ICSI operation of enough sperms the same day, even there will be the serious conditions without sperm in the seminal fluid taken out the same day, for avoiding this type of situation to occur, be an effective solution in advance by sperm cryopreservation.The method of this type of sperm freezing mostly is liquid nitrogen fumigating system at present, the steps include: seminal fluid and freezing liquid slowly to mix according to 1:1 volume, is contained in round bottom test tube, is stuck in freezing aluminum frame by round bottom test tube, is positioned over 4c
orefrigerator and cooled is 30min but, then drops into liquid nitrogen container after aluminum frame being put into above liquid nitrogen ware stifling 20min and preserve.The method has following drawback: 1, and mankind's ejaculation volume once is on average about about 2.5mL, adds freezing liquid volume about 5mL altogether, because sperm concentration is very low, is difficult to search out sperm when recovery in so many liquid; 2., because liquid volume in round bottom test tube is comparatively large, be difficult to time stifling liquid is caught a cold evenly, affect refrigerating effect; 3, it is shorter that sperm freezing aluminum frame length is sold in market, and can only place 2-3 round bottom test tube, efficiency is not high at every turn.
Utility model content
The purpose of this utility model in order to overcome deficiency of the prior art, thus provides a kind of novel sperm cryopreservation device being applicable to severe oligospermia.
A kind of novel sperm cryopreservation device being applicable to severe oligospermia, comprise hand lever, support, groove, freezing straw, tampon, adhesive label's paper, described hand lever is located on two symmetrical supports, two symmetrical supports are equipped with groove, freezing straw two ends are arranged in groove symmetrical on two supports, freezing straw one end is provided with tampon, tampon peripheral hardware toughness label paper.
The beneficial effect of employing technique scheme is:
1. the utility model is little due to freezing straw volume, is easier to find sperm at recovery sperm time ratio; Because in freezing straw, liquid is few, (about 0.4mL), when stifling, liquid is caught a cold relatively more even, and refrigerating effect is good; And many parts of semen samples can be fumigated simultaneously, increase efficiency.
2. the amount of liquid loaded in the every only freezing straw of the utility model is few, and time stifling, liquid is caught a cold evenly, and refrigerating effect is good, easily can find sperm during recovery, and once can fumigate many parts of semen samples, increases work efficiency.
Accompanying drawing explanation
Fig. 1 is the sperm cryopreservation device structural representation that the utility model is applicable to severe oligospermia.
Fig. 2 is that the utility model is applicable to freezing straw structural representation in the sperm cryopreservation device of severe oligospermia.
In figure: 1-hand lever, 2-support, 3-groove, the freezing straw of 4-, 5-tampon, 6-adhesive label paper.
Embodiment
Below in conjunction with accompanying drawing, the utility model is described in further detail:
A kind of novel sperm cryopreservation device being applicable to severe oligospermia, comprise hand lever 1, support 2, groove 3, freezing straw 4, tampon 5, adhesive label's paper 6, described hand lever 1 is located on two symmetrical supports 2, two symmetrical supports 2 are equipped with groove 3, freezing straw 4 two ends are arranged in groove 3 symmetrical on two supports 2, freezing straw 4 one end is provided with tampon 5, tampon 5 peripheral hardware toughness label paper 6.
Described freezing straw 4 capacity is 0.5mL.
This novel sperm cryopreservation device being applicable to severe oligospermia specifically uses step to be:
Seminal fluid is placed in centrifugal 10 minutes of conical pipe (1500 revs/min), abandon supernatant, leave and take precipitation 0.2mL, freezing liquid is pressed 1:1 volume slowly to mix with precipitation, again mixing material is sucked in freezing straw 4, adhesive label's paper 6 marks patient's name and numbering, uses sealing mud to be sealed by freezing straw 4.The freezing straw 4 of sealing is placed in 4c
orefrigerator and cooled is 30min but, is then stuck in groove 3, drops into liquid nitrogen container and preserve after being placed on liquid nitrogen ware stifling 20 min.
Claims (2)
1. the novel sperm cryopreservation device being applicable to severe oligospermia, it is characterized in that: it comprises hand lever (1), support (2), groove (3), freezing straw (4), tampon (5), adhesive label's paper (6), described hand lever (1) is located on two symmetrical supports (2), two symmetrical supports (2) are equipped with groove (3), freezing straw (4) two ends are arranged in two upper symmetrical grooves (3) of support (2), freezing straw (4) one end is provided with tampon (5), tampon (5) peripheral hardware toughness label paper (6).
2. the novel sperm cryopreservation device being applicable to severe oligospermia according to claim 1, is characterized in that: described freezing straw (4) capacity is 0.5mL.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201520155950.1U CN204499260U (en) | 2015-03-19 | 2015-03-19 | A kind of novel sperm cryopreservation device being applicable to severe oligospermia |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201520155950.1U CN204499260U (en) | 2015-03-19 | 2015-03-19 | A kind of novel sperm cryopreservation device being applicable to severe oligospermia |
Publications (1)
Publication Number | Publication Date |
---|---|
CN204499260U true CN204499260U (en) | 2015-07-29 |
Family
ID=53697279
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201520155950.1U Expired - Fee Related CN204499260U (en) | 2015-03-19 | 2015-03-19 | A kind of novel sperm cryopreservation device being applicable to severe oligospermia |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN204499260U (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106070186A (en) * | 2016-08-11 | 2016-11-09 | 王喜良 | The orientable frozen holder combination of biological specimen freezing carrier and using method |
CN115606580A (en) * | 2022-09-30 | 2023-01-17 | 斯贝福(北京)生物技术有限公司 | Continuous cryopreservation device and method for mouse sperms |
-
2015
- 2015-03-19 CN CN201520155950.1U patent/CN204499260U/en not_active Expired - Fee Related
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106070186A (en) * | 2016-08-11 | 2016-11-09 | 王喜良 | The orientable frozen holder combination of biological specimen freezing carrier and using method |
CN115606580A (en) * | 2022-09-30 | 2023-01-17 | 斯贝福(北京)生物技术有限公司 | Continuous cryopreservation device and method for mouse sperms |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
EP3560334A1 (en) | Cell freezing medium for clinical use | |
CN103039434B (en) | Duck egg yolk anti-freezing agent for cryopreservation of donkey semen and preparation method thereof | |
CN204499260U (en) | A kind of novel sperm cryopreservation device being applicable to severe oligospermia | |
CN101003791A (en) | Refrigeration method for preserving embryo and oocyte by vitrification of glasscapillary | |
CN103509680A (en) | Method for brewing persimmon ice wine | |
CN102669088B (en) | Mammal embryo and oocyte vitrification freezing carrier | |
CN208509955U (en) | A kind of miniature save set frozen for human testicle and epididymal sperm | |
CN104012521B (en) | A kind of contactless sessile drop method refrigerating plant and freezing method | |
CN107980766A (en) | A kind of method of donkey testicular fluid freezen protective | |
CN106804581B (en) | Device and method for preserving normal-temperature liquid-state pig semen under negative pressure | |
CN110432261A (en) | A kind of micro sperm freezing and defreezing method and its automatic fixture device | |
CN103392692A (en) | Cryopreservation solution for goat semen | |
CN109738589A (en) | A kind of method of rapidly extracting plant xylem sap | |
CN104086649A (en) | Method for extracting nutrients from spirulina in grading manner | |
CN101965829B (en) | Method for cryopreserving erythrocytes | |
CN115777696B (en) | Vitrification freezing thawing liquid suit | |
CN205196832U (en) | Freezing carrier of oocyte embryo glass ization | |
CN102246744A (en) | Giant panda semen freezing and preserving fluid and preparation method thereof | |
CN206949363U (en) | It is a kind of to be applied to freezing mankind's rareness sperm and the closed carrier of the double flute of embryo and ovum | |
CN103039433B (en) | Casein anti-freezing agent for cryopreservation of donkey semen and preparation method thereof | |
CN102258007A (en) | Refrigerant and method for storing Pacific codfish sperms | |
WO2012028967A2 (en) | Method for cryopreservation of human spermatozoa free from seminal plasma using a fast and simple aseptic vitrification-devitrification process; portable kit for carrying out the method; and use of the same for treatment of disorders related to reproductive failures | |
CN215075025U (en) | Single sperm freezing carrier | |
CN212589916U (en) | Trace sperm cryopreservation carrier | |
CN100405902C (en) | Frozen semen of animal and its making method |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
CF01 | Termination of patent right due to non-payment of annual fee | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150729 Termination date: 20190319 |