CN110432261A - A kind of micro sperm freezing and defreezing method and its automatic fixture device - Google Patents
A kind of micro sperm freezing and defreezing method and its automatic fixture device Download PDFInfo
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- CN110432261A CN110432261A CN201910823410.9A CN201910823410A CN110432261A CN 110432261 A CN110432261 A CN 110432261A CN 201910823410 A CN201910823410 A CN 201910823410A CN 110432261 A CN110432261 A CN 110432261A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0242—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
- A01N1/0252—Temperature controlling refrigerating apparatus, i.e. devices used to actively control the temperature of a designated internal volume, e.g. refrigerators, freeze-drying apparatus or liquid nitrogen baths
- A01N1/0257—Stationary or portable vessels generating cryogenic temperatures
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0608—Germ cells
- C12N5/061—Sperm cells, spermatogonia
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Abstract
The invention discloses a kind of micro sperm freezings and defreezing method and its automatic fixture device, the following steps are included: the freezing of S1, sperm, the defrosting of S2, sperm, the inside bottom of the liquid nitrogen box is placed with fixed frame, promotion motor is fixed at the top of the fixed frame, the output shaft for promoting motor is fixedly connected with the lifting handle through fixed frame；The present invention can carry bar to more freezings and freeze simultaneously, operate more convenient, efficient quick, and the sperm droplet on freezing load bar is stifling more evenly by condenser, more acurrate, and the Cryopreservation rate of micro sperm is greatly improved；Reduce the fault and frostbite of operator again simultaneously；The present invention has well solved seriously few weak sperm, testis, the epididymal sperm occurred in current spermatozoa cryopreservation technology and has frozen the problem that rear sperm recycling is easy failure, and the flexibility for making azoospermia patients receive assisted reproduction treatment is greatly improved.
Description
Technical field
The invention belongs to human ancillary reproductive fields, and in particular to a kind of micro sperm freezing and defreezing method and its automatic
Grip device.
Background technique
Environmental pollution is on the rise, hormone abuse etc. causes mankind spermatozoon quality and quantity constantly to decline, male sterility ratio
Example increasingly increases, and the infertility as caused by male sterility problem accounts about 40-50% in world wide, and azoospermia accounts about
The 10% of male sterility problem affects the males in the whole world 1%, and the incidence of azoospermia can gradually rise.
Azoospermia patients had been the child that can not possess in oneself science of heredity meaning in the past, and with assisted reproductive technology
Development, azoospermia patients can be by Percutaneous epididymal sperm aspiration (PESA), Testicular sperm extraction (TESA), micro-
The technologies such as Testicular sperm extraction (MESE) obtain a small amount of sperm, then by intracytoplasmic sperm injection (ICSI) will be smart
Son is injected into egg mother cell, finally will obtain clinical pregnancy in the embryo transfer of formation to women uterine cavity.But taking repeatedly
Smart art may cause testis, epididymis tissue fibrosis, and orchiatrophy, spermatogenesis is caused to be degenerated.Therefore by a small amount of motile sperm
Freezen protective gets up, and has not only mitigated patient because puncturing brought pain and financial burden repeatedly, but also can largely keep away
Exempt from that ovum day is taken to cancel fertilization due to can not be with sperm.
Current micro sperm freezing is a variety of methods such as free oolemma, freezing ring, ICSI needle, miniature straw, but lacks
Practicability.Traditional sperm freezing using 1.8mL cryovial (Cryovial) be used as freezing carrier, sperm recovery survival rate,
The rate of recovery is all very low, so not being suitable for carrying out freezen protective to the sperm for puncturing acquisition.
Once Chinese patent application 200810042695.4 provides the freezing and defreezing method and essence of a kind of micro sperm
The freezing of son and thawing apparatus, but this method needs to manufacture the dedicated wet box of saturated humidity condition, it is micro- in refrigerating process to guarantee
Drop is not evaporated, and operating process is cumbersome, time-consuming, and this strongly limits the invention being widely used clinically.
Summary of the invention
The purpose of the present invention is to provide a kind of micro sperm freezings and defreezing method and its automatic fixture device, to solve
Problem mentioned above in the background art.
To achieve the above object, present invention employs following technical solutions:
A kind of micro sperm freezing and defreezing method, comprising the following steps:
The freezing of S1, sperm: sperm is enriched with first after optimizing, then sperm and cryoprotector 1:1 are obtained after mixing
Freezing drop is placed in spare under room temperature, injects liquid nitrogen into liquid nitrogen box, so that liquid nitrogen injection rate is the half of liquid nitrogen box
Volume carries bar to freezing using automatic fixture device and clamps, and freezing load bar, will be mixed apart from liquefied ammonia steam face 20cm
It freezes drop to instill with 5-10uL amount in the piece portion for freezing load bar, be declined by automatic fixture device with 5 cm/ minutes speed
To 5 position cm of liquid nitrogen surface, then freezing was carried by bar with 1 cm/ minutes speed and is fallen into liquid nitrogen, carries out long-term preservation；
The defrosting of S2, sperm: carrying out preceding preparation of thawing first, carries out pre-temperature 30min to the buffering culture solution of mineral oil covering, makes
Culture-liquid temp must be buffered to stablize at 34-37 degrees Celsius, freezing load bar is removed from liquid nitrogen and was built in 1-3 seconds time pre-
Rewarming thaws in the buffering culture solution that temperature is completed, for monosperm ooecium slurry injection ICSI.
Preferably, in S1, automatic fixture device is fallen into liquid nitrogen according to the load bar that will freeze of specified speed at the uniform velocity
Step are as follows: lifting pull-up handle to pull up handle far from upper clamp plate upwards first, and freezing is carried in bar insertion fillet clamping slot,
So that freezing carries bar and is clamped elastic slice clamping fixed, pull-up handle is then unclamped, makes lower clamp plate under the pulling of fastening spring
Bar is carried to freezing with upper clamp plate to grip, and the piece portion of freezing load bar and liquid nitrogen vapor face is kept to tilt at 15 degree of angles
State is placed, and is then at the uniform velocity rotated, is mentioned clockwise promoting motor driven lifting handle made of polytetrafluoroethylene material
It drives guide rod to carry out uniform descent under the guiding of guide post when rising screw rod rotation, carries bar in freezing and be completely immersed in liquid nitrogen box
Liquid nitrogen in after stop promoted motor work, after the completion of sperm freezing, by outer tube be packed into carry bar, then by pull-up handle will
Upper clamp plate is promoted, and the carrier for being loaded with sperm is detached from grip device naturally and enters progress long term storage in liquid nitrogen.
Preferably, in S2, freezing is carried into the step of bar is removed from liquid nitrogen are as follows: verification patient Mr. and Mrs label first is correct, will
Freezing carries bar and is transferred in liquid nitrogen box from liquid nitrogen container, and freezing load bar is taken out in liquid nitrogen box and was built in pre-temperature in 1-3 seconds time and is completed
Buffering culture solution in rewarming thaw, until freezing liquid drop be fully immersed into culture solution, microscopically observation sperm motility and shape
State, chooses that form is normal and great-hearted sperm is for monosperm ooecium slurry injection ICSI.
In addition present invention further provide that a kind of automatic fixture device, including liquid nitrogen box and freezing carry bar, the liquid nitrogen box
Inside bottom is placed with fixed frame, and promotion motor is fixed at the top of the fixed frame, and the output shaft for promoting motor is fixed
It is connected with the lifting handle through fixed frame, the inside two of the fixed frame is fixed with guide post, two guide posts point
Not Guan Chuan lower clamp plate two sides, be fixed with guide rod at the top of the lower clamp plate, the outside sliding sleeve of the guide rod is connected to set
Pipe, the bottom and top of described sleeve pipe are respectively fixed with the upper clamp plate being socketed on the outside of guide rod and pull-up handle, the upper folder
The two sides of the bottom of plate pass through the top that fastening spring is connected to lower clamp plate, and threaded hole, institute are offered at the top of the guide rod
It states lifting handle to be threadedly coupled with threaded hole, the fixation handle being socketed on the outside of lifting handle is fixed at the top of the guide rod
Hand is uniformly provided with fillet clamping slot at the top of the lower clamp plate, and two inner sidewalls of the fillet clamping slot are respectively and fixedly provided with clamping
Elastic slice, the freezing carry bar and are inserted in fillet clamping slot, and the fillet clamping slot is fitted closely with elastic slice is clamped, the freezing
Carrying bar includes piece portion and bar portion.
Preferably, two lateral roofs of the lower clamp plate offer the pilot hole to match with guide post, the guide post
It is inserted in pilot hole.
Preferably, the fixed frame includes that the first support column, transverse slat, pedestal and station foot, the two sides of the bottom of the pedestal are equal
It is fixed with station foot, the top two sides of the pedestal are respectively and fixedly provided with the first support column, are fixed on cross at the top of first support column
The bottom of plate.
Preferably, the bottom of transverse slat is fixed at the top of the guide post, pedestal is fixed in the bottom of the guide post
Top.
Preferably, the inner cavity size of the fillet clamping slot are as follows: a height of 2.4mm, width 2.4mm.
Preferably, described portion is that long sheet or channel-shaped are arranged, it is described clamp elastic slice, the material of fastening spring is
304 stainless steels.
Preferably, the material of the fixed frame, fixed handle, pull-up handle, casing, upper clamp plate, lower clamp plate and guide post
It is polytetrafluoroethylene material.
Technical effect and advantage of the invention: a kind of micro sperm freezing and defreezing method proposed by the present invention and its automatic
Grip device has the advantage that compared with prior art
1, the present invention can carry bar to more freezings and freeze simultaneously, operate more convenient, efficient quick, and freezing carries the essence on bar
Sub- droplet is stifling more evenly by condenser, more acurrate, and the Cryopreservation rate of micro sperm is greatly improved；Reduce operation again simultaneously
The fault and frostbite of personnel；
2, present invention freezing carries bar front end in the form of sheets or channel-shaped loads sperm, and sperm is placed in the carrier droplet of front end and freezes, In
Rewarming thaws in the buffering culture solution of the mineral oil covering of heat, and the sperm rate of recovery is close to 100%.Automatic fixture device can be primary
Property load 8 freezings and carry bars, the more convenient, efficient quick of operation reduces the fault and frostbite of operator.The present invention is well
It solves seriously few weak sperm, testis, the epididymal sperm occurred in current spermatozoa cryopreservation technology and freezes the rear easy mistake of sperm recycling
The problem lost, the flexibility for making azoospermia patients receive assisted reproduction treatment are greatly improved.
Detailed description of the invention
Fig. 1 is the structural diagram of the present invention；
Fig. 2 is fixed rack structure schematic diagram of the invention；
Fig. 3 is lower clamp plate structural schematic diagram of the invention；
Fig. 4 is clamping elastic piece structure schematic diagram of the invention；
Fig. 5 is that freezing of the invention carries rod structure schematic diagram.
In figure: 1, liquid nitrogen box；2, fixed frame；3, the first support column；4, transverse slat；5, motor is promoted；6, lifting handle；7, solid
Determine handle；8, guide rod；9, handle is pulled up；10, casing；11, upper clamp plate；12, lower clamp plate；13, guide post；14, fastening spring；
15, pilot hole；16, fillet clamping slot；17, threaded hole；18, elastic slice is clamped；19, freezing carries bar；20, pedestal；21, it stands foot；22,
Piece portion；23, bar portion.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Herein
Described specific embodiment is only used to explain the present invention, is not intended to limit the present invention.Based on the embodiments of the present invention,
Every other embodiment obtained by those of ordinary skill in the art without making creative efforts, belongs to this hair
The range of bright protection.
Embodiment
The present invention provides a kind of micro sperm freezing as shown in Figs. 1-5 and defreezing methods, comprising the following steps:
The freezing of S1, sperm: sperm is enriched with first after optimizing, then sperm and cryoprotector 1:1 are obtained after mixing
Freezing drop is placed in spare under room temperature, injects liquid nitrogen into liquid nitrogen box, so that liquid nitrogen injection rate is the half of liquid nitrogen box
Volume carries bar to freezing using automatic fixture device and clamps, and freezing load bar, will be mixed apart from liquefied ammonia steam face 20cm
It freezes drop to instill with 5-10uL amount in the piece portion for freezing load bar, be declined by automatic fixture device with 5 cm/ minutes speed
To 5 position cm of liquid nitrogen surface, then freezing was carried by bar with 1 cm/ minutes speed and is fallen into liquid nitrogen, after the completion of sperm freezing, peace
Dress freezing carries bar outer tube, is pulled open upper clamp plate by pull-up handle, load bar is detached from naturally sets freeze box, and grows to sperm
Phase saves；Automatic fixture device falls into the step in liquid nitrogen according to the load bar that will freeze of specified speed at the uniform velocity are as follows: first upwards
Lifting pull-up handle to pull up handle far from lower clamp plate, and freezing is carried in bar insertion fillet clamping slot, primary to can be inserted into 8 loads
Then bar unclamps pull-up handle, lower folder is made under the pulling of fastening spring so that freezing carries bar and is clamped elastic slice clamping fixed
Plate and upper clamp plate carry bar to freezing and grip, and the piece portion for keeping freezing to carry bar inclines with liquid nitrogen vapor face at 15 degree of angles
Ramp-like state is placed, and is then at the uniform velocity rotated clockwise promoting motor driven lifting handle made of polytetrafluoroethylene material,
It promotes motor and selects totally-enclosed self cooling stainless steel motor, drive guide rod to carry out under the guiding of guide post when lifting handle rotates
Uniform descent carries in freezing and stops promoting motor work after bar is completely immersed in the liquid nitrogen in liquid nitrogen box, completes to sperm freezing
Afterwards, outer tube is packed into and carries bar, then promoted upper clamp plate by pull-up handle, the carrier for being loaded with sperm is detached from grip device naturally
Long term storage is carried out in into liquid nitrogen；
The defrosting of S2, sperm: carrying out preceding preparation of thawing first, carries out pre-temperature 30min to the buffering culture solution of mineral oil covering, makes
Culture-liquid temp must be buffered to stablize at 34-37 degrees Celsius, freezing load bar is removed from liquid nitrogen and was built in 1-3 seconds time pre-
Rewarming thaws in the buffering culture solution that temperature is completed, until freezing liquid drop is fully immersed into culture solution, microscopically observation sperm is living
Power and form, choose that form is normal and great-hearted sperm is for monosperm ooecium slurry injection ICSI.
In addition present invention further provide that a kind of automatic fixture device, including liquid nitrogen box 1 and freezing carry bar 19, the liquid nitrogen
The inside bottom of box 1 is placed with fixed frame 2, and the top of the fixed frame 2, which is fixed with, promotes motor 5, described to promote the defeated of motor 5
Shaft is fixedly connected with the lifting handle 6 through fixed frame 2, and the inside two of the fixed frame 2 is fixed with guide post 13, and two
The guide post 13 extends through the two sides of lower clamp plate 12, and the top of the lower clamp plate 12 is fixed with guide rod 8, the guide rod
8 outside sliding sleeve is connected to casing 10, and the bottom and top of described sleeve pipe 10, which are respectively fixed with, is socketed on the upper of 8 outside of guide rod
Clamping plate 11 and pull-up handle 9, the two sides of the bottom of the upper clamp plate 11 pass through the top that fastening spring 14 is connected to lower clamp plate 12,
The top of the guide rod 8 offers threaded hole 17, and the lifting handle 6 is threadedly coupled with threaded hole 17, the guide rod 8
Top is fixed with the fixation handle 7 for being socketed on 6 outside of lifting handle, and the top of the lower clamp plate 12 is uniformly provided with fillet clamping
Slot 16, two inner sidewalls of the fillet clamping slot 16, which are respectively and fixedly provided with, clamps elastic slice 18, and the freezing carries bar 19 and is inserted in fillet folder
In tight slot 16, the fillet clamping slot 16 is fitted closely with elastic slice 18 is clamped, and it includes piece portion 22 and bar portion that the freezing, which carries bar 19,
23.The size in piece portion 22 is the freezing liquid droplet that can carry 5.0-10.0uL, and freezing is carried bar 19 and is made with polystyrene material.
When carrying out sperm freezing operation, 8 freezings are carried into bar 19 and are successively plugged in fillet folder in the case where being detached from liquid nitrogen vapor surface layer state
In tight slot 16, freezing load bar 19 is clamped by clamping elastic slice 18 and fastening spring 14, sperm will be loaded with by lifting handle 6
Freezing carries to be freezed in the feeding liquid nitrogen box 1 of bar 19, after the completion of sperm freezing, is promoted upper clamp plate 11 by pull-up handle 9,
The freezing for being loaded with sperm, which carries bar 19 and is detached from grip device naturally and enters in liquid nitrogen, to be stored.Two lateral roofs of the lower clamp plate 12
The pilot hole 15 to match with guide post 13 is offered, the guide post 13 is inserted in pilot hole 15.The fixed frame 2 wraps
The first support column 3, transverse slat 4, pedestal 20 and station foot 21 are included, the two sides of the bottom of the pedestal 20 are respectively and fixedly provided with station foot 21, the bottom
The top two sides of seat 20 are respectively and fixedly provided with the first support column 3, and the bottom of transverse slat 4 is fixed at the top of first support column 3.It is described
The bottom of transverse slat 4 is fixed at the top of guide post 13, and the top of pedestal 20 is fixed in the bottom of the guide post 13.The fillet
The inner cavity size of clamping slot 16 are as follows: a height of 2.4mm, width 2.4mm.Described portion 22 is that long sheet or channel-shaped are arranged, described
Clamp elastic slice 18, the material of fastening spring 14 is 304 stainless steels.The fixed frame 2, fixed handle 7, pull-up handle 9, casing
10, the material of upper clamp plate 11, lower clamp plate 12 and guide post 13 is polytetrafluoroethylene material, and freezing carries bar 19 and uses polystyrene
It is made.
Working principle: to pull up handle far from lower clamp plate pull-up handle will be lifted upwards first, freezing is carried into bar 19
It is inserted into fillet clamping slot 16,8 fillet clamping slots 16 is set altogether, it is primary to can be inserted into 8 freezings load bars 19, so that freezing carries bar
Then 19 clamped 18 clamping fixeds of elastic slice unclamp pull-up handle 9, lower clamp plate 12 and upper are made under the pulling of fastening spring 14
11 pairs of freezings of clamping plate carry bar 19 and grip, and the piece portion 22 and liquid nitrogen vapor face for keeping freezing to carry bar 19 are at 15 degree of angles
Heeling condition is placed, and then drives the lifting handle 6 made of polytetrafluoroethylene material at the uniform velocity to revolve clockwise promoting motor 5
Turn, promotes motor 5 and select totally-enclosed self cooling stainless steel motor, the leading in guide post 13 of guide rod 8 is driven when lifting handle 6 rotates
Uniform descent is carried out downwards, and freezing carries bar 19 and drops in liquid nitrogen, puts on outer tube, and by raising pull-up handle 9, freezing carries bar
19 are detached from automatic fixture device naturally and are placed in liquid nitrogen box 1, are packed into bracket and are transferred to liquid nitrogen container progress long-term preservation.
Finally, it should be noted that the foregoing is only a preferred embodiment of the present invention, it is not intended to restrict the invention,
Although the present invention is described in detail referring to the foregoing embodiments, for those skilled in the art, still may be used
To modify the technical solutions described in the foregoing embodiments or equivalent replacement of some of the technical features,
All within the spirits and principles of the present invention, any modification, equivalent replacement, improvement and so on should be included in of the invention
Within protection scope.
Claims (10)
1. a kind of micro sperm freezing and defreezing method, it is characterised in that: the following steps are included:
The freezing of S1, sperm: sperm is enriched with first after optimizing, then sperm and cryoprotector 1:1 are obtained after mixing
Freezing drop is placed in spare under room temperature, injects liquid nitrogen into liquid nitrogen box, so that liquid nitrogen injection rate is the half of liquid nitrogen box
Volume carries bar to freezing using automatic fixture device and clamps, and freezing load bar, will be mixed apart from liquefied ammonia steam face 20cm
It freezes drop to instill with 5-10uL amount in the piece portion for freezing load bar, be declined by automatic fixture device with 5 cm/ minutes speed
To 5 position cm of liquid nitrogen surface, then freezing was carried by bar with 1 cm/ minutes speed and is fallen into liquid nitrogen, carries out long-term preservation；
The defrosting of S2, sperm: carrying out preceding preparation of thawing first, carries out pre-temperature 30min to the buffering culture solution of mineral oil covering, makes
Culture-liquid temp must be buffered to stablize at 34-37 degrees Celsius, freezing load bar is removed from liquid nitrogen and was built in 1-3 seconds time pre-
Rewarming thaws in the buffering culture solution that temperature is completed, for monosperm ooecium slurry injection ICSI.
2. a kind of micro sperm freezing according to claim 1 and defreezing method, it is characterised in that: automatic to press from both sides in S1
Tool device falls into the step in liquid nitrogen according to the load bar that will freeze of specified speed at the uniform velocity are as follows: lifting pull-up handle makes upwards first
Handle must be pulled up and consolidated in freezing load bar insertion fillet clamping slot so that freezing load bar and being clamped elastic slice clamping far from upper clamp plate
Determine, then unclamp pull-up handle, makes lower clamp plate and upper clamp plate carry bar to freezing under the pulling of fastening spring and clamp admittedly
It is fixed, it keeps the piece portion of freezing load bar and liquid nitrogen vapor face to place at 15 degree of angle heeling conditions, is then driven by promoting motor
The dynamic lifting handle made of polytetrafluoroethylene material at the uniform velocity rotates clockwise, drives guide rod being oriented to when lifting handle rotates
Uniform descent is carried out under the guiding of column, is carried in freezing and is stopped promoting motor work after bar is completely immersed in the liquid nitrogen in liquid nitrogen box,
After the completion of sperm freezing, outer tube is packed into and carries bar, then promoted upper clamp plate by pull-up handle, is loaded with the carrier of sperm certainly
It is so detached from grip device and enters progress long term storage in liquid nitrogen.
3. a kind of micro sperm freezing according to claim 1 and defreezing method, it is characterised in that: in S2, will freeze
Carry the step of bar is removed from liquid nitrogen are as follows: verification patient Mr. and Mrs label first is correct, and freezing is carried bar and is transferred to liquid nitrogen from liquid nitrogen container
Freezing is taken out in box, in liquid nitrogen box and carries bar and the rewarming defrosting in the buffering culture solution that 1-3 seconds time was built in pre-temperature completion, directly
It is fully immersed into culture solution to freezing liquid drop, it is normal and great-hearted to choose form for microscopically observation sperm motility and form
Sperm is for monosperm ooecium slurry injection ICSI.
4. a kind of automatic fixture device according to claim 1 to 3, it is characterised in that: including liquid nitrogen box (1)
Bar (19) are carried with freezing, the inside bottom of the liquid nitrogen box (1) is placed with fixed frame (2), and the top of the fixed frame (2) is fixed
Have promotion motor (5), the output shaft for promoting motor (5) is fixedly connected with the lifting handle (6) through fixed frame (2), institute
The inside two for stating fixed frame (2) is fixed with guide post (13), and two guide posts (13) extend through lower clamp plate (12)
Two sides are fixed with guide rod (8) at the top of the lower clamp plate (12), and the outside sliding sleeve of the guide rod (8) is connected to casing
(10), the bottom and top of described sleeve pipe (10) are respectively fixed with the upper clamp plate (11) being socketed on the outside of guide rod (8) and pull-up
Handle (9), the two sides of the bottom of the upper clamp plate (11) pass through the top that fastening spring (14) is connected to lower clamp plate (12), described
It is offered at the top of guide rod (8) threaded hole (17), the lifting handle (6) is threadedly coupled with threaded hole (17), the guiding
The fixation handle (7) being socketed on the outside of lifting handle (6) is fixed at the top of bar (8), the top of the lower clamp plate (12) is uniform
It offers fillet clamping slot (16), two inner sidewalls of the fillet clamping slot (16), which are respectively and fixedly provided with, clamps elastic slice (18), described cold
Freeze load bar (19) to be inserted in fillet clamping slot (16), the fillet clamping slot (16) fits closely with elastic slice (18) are clamped, institute
Stating freezing and carrying bar (19) includes piece portion (22) and bar portion (23).
5. a kind of automatic fixture device according to claim 4, it is characterised in that: two lateral roofs of the lower clamp plate (12)
The pilot hole (15) to match with guide post (13) is offered, the guide post (13) is inserted in pilot hole (15).
6. a kind of automatic fixture device according to claim 4, it is characterised in that: the fixed frame (2) includes first
Dagger (3), transverse slat (4), pedestal (20) and station foot (21), the two sides of the bottom of the pedestal (20) are respectively and fixedly provided with station foot (21), institute
The top two sides for stating pedestal (20) are respectively and fixedly provided with the first support column (3), are fixed on transverse slat at the top of first support column (3)
(4) bottom.
7. a kind of automatic fixture device according to claim 4, it is characterised in that: the top of the guide post (13) is fixed
In the bottom of transverse slat (4), the top of pedestal (20) is fixed in the bottom of the guide post (13).
8. a kind of automatic fixture device according to claim 4, it is characterised in that: the inner cavity of the fillet clamping slot (16)
Size are as follows: a height of 2.4mm, width 2.4mm.
9. a kind of automatic fixture device according to claim 4, it is characterised in that: described portion (22) be long sheet or
Channel-shaped setting, it is described clamp elastic slice (18), fastening spring (14) material be 304 stainless steels.
10. a kind of automatic fixture device according to claim 4, it is characterised in that: the fixed frame (2), fixed handle
(7), the material for pulling up handle (9), casing (10), upper clamp plate (11), lower clamp plate (12) and guide post (13) is polytetrafluoroethyl-ne
Alkene material.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910823410.9A CN110432261A (en) | 2019-09-02 | 2019-09-02 | A kind of micro sperm freezing and defreezing method and its automatic fixture device |
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| CN201910823410.9A CN110432261A (en) | 2019-09-02 | 2019-09-02 | A kind of micro sperm freezing and defreezing method and its automatic fixture device |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112616828A (en) * | 2020-12-21 | 2021-04-09 | 武汉华联科生物技术有限公司 | Mouse sperm freezes deposits gradient cooling platform |
| CN112961775A (en) * | 2021-03-10 | 2021-06-15 | 秦娟 | Cell recovery device |
| CN115606580A (en) * | 2022-09-30 | 2023-01-17 | 斯贝福（北京）生物技术有限公司 | Continuous cryopreservation device and method for mouse sperms |
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| CN115606580B (en) * | 2022-09-30 | 2024-06-11 | 斯贝福（北京）生物技术有限公司 | Mouse sperm continuous freezing device and freezing method |
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