CN204356338U - Be applied to the side luminous optical detection system of PCR instrument - Google Patents
Be applied to the side luminous optical detection system of PCR instrument Download PDFInfo
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- CN204356338U CN204356338U CN201420804189.5U CN201420804189U CN204356338U CN 204356338 U CN204356338 U CN 204356338U CN 201420804189 U CN201420804189 U CN 201420804189U CN 204356338 U CN204356338 U CN 204356338U
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Abstract
The utility model discloses a kind of side luminous optical detection system being applied to PCR instrument, comprise retaining plate, exciting light system, transparent agent pipe and camera, retaining plate is horizontally disposed with, exciting light system comprises optics permanent seat, illuminator, light-guiding pillar and guide-lighting groove, optics permanent seat is connected with retaining plate, illuminator and light-guiding pillar are all positioned at optics permanent seat, and illuminator and light-guiding pillar are all parallel with retaining plate, be provided with and excite optical filtering between illuminator and light-guiding pillar; The bottom of guide-lighting groove is provided with opening and guide-lighting groove is connected with retaining plate is vertical, and camera is positioned at the below of guide-lighting groove; The sidewall of guide-lighting groove is provided with the through hole with light-guiding pillar vertical connection.It is complicated that the utility model overcomes existing PCR instrument light path design, part position precision is high, use and maintenance cost is high, the shortcoming of maintenance difficulty, provides that a kind of volume is little, non-maintaining, long service life and the low side luminous optical detection system being applied to PCR instrument of cost.
Description
Technical field
The utility model belongs to biochemical field, is specifically related to a kind of side luminous optical detection system being applied to PCR instrument.
Background technology
In biology field, PCR reaction is conventional experimental technique, PCR reaction and polymerase chain reaction (Polymerase Chain Reaction, PCR), it is a kind of method of external enzyme' s catalysis specific DNA fragment, to be recombinated by high-temperature denatured, low temperature and thermophilic extends and forms one-period, this cycle of recirculation, target DNA is made to be able to rapid amplification, there is high specificity, highly sensitive, easy and simple to handle, the feature such as save time, it not only can be used for the fundamental researchs such as gene isolation, clone and nucleic acid sequence analysis, also can be used for the diagnosis of disease.
The instrument realizing fluorescence real-time quantitative PCR technology is fluorescence real-time quantitative PCR instrument, conventional PCR instrument, such as, China Patent Publication No. is CN 201785391 U, principle of work is: illuminator sends exciting light by top, being arrived by optics chunks such as filters has on 96 orifice plates of sample, the fluorescence launched in sample returns according to former road, at dichroic mirror place, because wavelength is different from incident light, therefore, filter wheel can be arrived through this mirror, filter wheel to rotate the parts selecting different filter, the light of specific wavelength can be made to pass through, thus reach the object distinguishing product, the fluorescence of being taken turns by filter is caught by the optical receiving system that instrument carries after polynary mirror, fluorescent PCR common are camera, PMT(photomultiplier) receive detection mode, thus produce a fluorescent signal, input computer carries out computational analysis.
The optical system of conventional PCR instrument is positioned at top, so the Reagent Tube analyzed and agent plate all must meet the condition of top high-transmittance, light path design is complicated, in addition, adds that excitation light source is vertical with camera, thus to a certain extent, distance demand fulfillment certain condition between light source and optical detection device, thus limited to the volume of PCR instrument on the one hand, take up room larger, on the other hand, too high to the status requirement precision of part; Moreover, the excitation light source of optical system is the top of agent plate, reagent must could be added by mobile optical excitation apparatus when again adding reagent, and current optical excitation device and support are chain connection, but repeatedly bend hinge to the high precision path of light path and unfavorable, therefore, usually need after long-time use to carry out auxiliary correction to optical system, could meet high-precision requirement, maintenance cost is high, the life-span is short; Also have, the optical mirror slip of the optical system of existing PCR instrument is exposed in physical environment, be vulnerable to the pollution of dust in air and other particulate matter, optical path is complicated, part difficulty of processing is large, after instrument frequently uses, needs regularly to carry out clean maintenance to instrument, and then optical system is recalibrated, use cost is high.
Utility model content
The technical problems to be solved in the utility model is complicated for existing PCR instrument light path design, part position precision is high, use and maintenance cost is high, the shortcoming of maintenance difficulty, provides that a kind of light path is simple, processing requirement is low, volume is little, non-maintaining, long service life and the low side luminous optical detection system being applied to PCR instrument of cost.
In order to solve the problems of the technologies described above, the utility model provides following technical scheme: the side luminous optical detection system being applied to PCR instrument, comprises exciting light system, guide-lighting groove, fluorescence reception detection system and horizontally disposed retaining plate;
Exciting light system comprises optics permanent seat, illuminator and light-guiding pillar, and optics permanent seat is connected with retaining plate, and illuminator and light-guiding pillar are all positioned at optics permanent seat, and illuminator, light-guiding pillar are all parallel with retaining plate;
The bottom of guide-lighting groove is provided with opening and guide-lighting groove is vertically connected with retaining plate, and the sidepiece of guide-lighting groove offers the through hole be communicated with light-guiding pillar, is vertically provided with Reagent Tube in guide-lighting groove;
Be provided with between illuminator and guide-lighting groove through hole and excite optical filtering;
Fluorescence reception detection system comprises the camera be vertically positioned at below guide-lighting groove, is provided with reception optical filtering between camera and guide-lighting groove.
Adopt the side luminous optical detection system being applied to PCR instrument of technical solutions of the utility model, comprise exciting light system, guide-lighting groove, fluorescence reception detection system and horizontally disposed retaining plate, retaining plate plays fixed action.Exciting light system comprises optics permanent seat, illuminator and light-guiding pillar, and optics permanent seat is connected with retaining plate, and illuminator and light-guiding pillar are all positioned at optics permanent seat, and illuminator, light-guiding pillar are all parallel with retaining plate; Light source and light-guiding pillar are positioned at closed optics permanent seat, and give the light path that illuminator is closed, avoid mixed and disorderly light path to occur, in addition, illuminator and light-guiding pillar are positioned at optics permanent seat, from the pollution of the impurity such as external dust, thus can avoid maintenance; Illuminator and light-guiding pillar are all parallel with retaining plate, ensure the trend of light path.
The bottom of guide-lighting groove is provided with opening and guide-lighting groove is vertically connected with retaining plate, and the sidepiece of guide-lighting groove offers the through hole be communicated with light-guiding pillar, is vertically provided with Reagent Tube in guide-lighting groove; Guide-lighting groove is on the one hand for placing Reagent Tube, and the through hole that the guide-lighting groove sidepiece of another aspect is offered will be for receiving and the light of light source activation be guided to Reagent Tube.
Be provided with between illuminator and guide-lighting groove through hole and excite optical filtering; Excite optical filtering for the veiling glare in the light of filter light source excitation, thus choose and required excite wave band.
Fluorescence reception detection system comprises the camera be vertically positioned at below guide-lighting groove, is provided with reception optical filtering between camera and guide-lighting groove; The fluorescent signal that camera sends for the reagent obtained in the Reagent Tube that is positioned in guide-lighting groove, receive optical filtering then for crossing the veiling glare signal outside fluorescent signal that filtering Reagent Tube sends, make camera obtain image, preserve the true fluorescence data in current Reagent Tube.
Principle of work of the present utility model is: the illuminator emission of light being positioned at the optics permanent seat of guide-lighting groove side, light is through exciting optical filtering to filter, by light-guiding pillar, required exciting light is passed in perpendicular guide-lighting groove, thus the transparent agent pipe be positioned in guide-lighting groove receives exciting light from the side, fluorescent reaction liquid in Reagent Tube receives exciting light, send fluorescence, fluorescence vertically takes in camera by the bottom opening of guide-lighting groove through the filtration receiving optical filtering by the bottom of transparent agent pipe again, camera collection data are also analyzed it, obtain fluoroscopic examination information.
The beneficial effects of the utility model are: the first, and exciting light system of the present utility model is exciting light, excites optical filtering, light-guiding pillar, reagent to be positioned at same straight line; Reagent utilizing emitted light, reception optical filtering, camera are positioned at same straight line, and comparatively plan vertical exciting light system light path is simple.
The second, due to the side excitation light source from Reagent Tube, therefore, the requirement to Reagent Tube and agent plate top height light transmissive material can be exempted, widened the selection face of Reagent Tube and agent plate top seal material, made the design and select material of Reagent Tube and agent plate more flexible, processing requirement reduces.
3rd, compared with the detection system of existing plan vertical excitation light source, side excitation light source can reduce device height to a certain extent, thus can reduce volume, and product is easy to carry.
4th, side excitation light source, when adding reagent, compared with the excitation light source of existing top, avoid repeatedly moving when reagent adding the damage that excitation light source device causes, side of the present utility model excitation light source without the need to movement, even if under the long-time frequent prerequisite used, also without the need to correcting optical system, reduce maintenance cost, increase the service life, in lasting use procedure, its optical property is consistent, and stability is strong.
5th, the exciting light sources of side of the present utility model excitation light source, excite optical filtering, light-guiding pillar is built in optics permanent seat, without exposing, dust not easily in ingress of air and other to the influential particulate matter of optics, avoid being polluted and having an impact to analytical results, make optical system more reliable and more stable.
Further, described reception optical filtering replaces with reception filter coating; Described excite optical filtering to replace with to excite filter coating.Receive filter coating by receiving optical filtering and exciting optical filtering to replace with and excite filter coating can realize the effect of filter light equally.
Further, describedly excite optical filtering or excite filter coating between light source and light-guiding pillar or be positioned at light-guiding pillar.Through exciting optical filtering or exciting the filtration of filter coating after light source activation light, excitation light irradiation after filtration is in the Reagent Tube in guide-lighting groove, therefore, no matter excite optical filtering or excite filter coating between light source and light-guiding pillar or be positioned at light-guiding pillar, as long as ensure that exciting light carried out filtration and can realize before entering the Reagent Tube in guide-lighting groove, during use, excite optical filtering to be installed between light-guiding pillar or be installed in light-guiding pillar, exciting filter coating then to need to be coated on transmitting substrate and install again.
Further, described optics permanent seat comprises optics female seat and optics female seat extender, illuminator is positioned at optics female seat, light-guiding pillar is positioned at optics female seat extender, optics female seat and optics female seat extended seat removably connect, and excite optical filtering or excite filter coating between optics female seat and optics female seat extender.Optics permanent seat is formed by connecting by two sections, i.e. optics female seat and optics female seat extender, optical filtering is excited to be installed between the two, filter coating is excited then to need to be coated on transmitting substrate and install, replacing for convenience detach excites optical filtering or excites filter coating, filters the light of invalid wave band, makes the fluorophor on the photoactivated dna target of effective wave band, and the length of optics female seat extender can be changed according to practical situation, to use different demand, more convenient operation, safeguard more convenient.
Further, describedly excite optical filtering or excite filter coating to be positioned at the end of optics female seat.Dismounting is rear easily changes optics female seat extender, excites optical filtering or excite filter coating also to facilitate to take out replacing.
Further, describedly excite optical filtering or excite filter coating to be positioned at the end of optics female seat extender.Same reason, the convenient equally taking-up in end exciting optical filtering or excite filter coating to be installed in optics female seat extender is changed.
Further, described illuminator is LED, halogen tungsten lamp or halogen lamp.LED is the abbreviation of English light emitting diode (photodiode); its basic structure is one piece of electroluminescent chip of semiconductor material; with elargol or latex solidified on support; then chip and circuit card is connected with silver-colored line or gold thread; then surrounding epoxy sealing; play the effect of protection internal core, last mounting casing, so the anti-seismic performance of LED lamp is good.Halogen tungsten lamp (halogen lamp) is containing part haloid element or halid gas incandescent lamp in blanketing gas.Halogen bulb (Halogen lamp), also known as tungsten sodium lamp bubble, is the one of incandescent light.LED, halogen tungsten lamp or halogen lamp all can be passed through the exciting light exciting the filtration of optical filtering to obtain required wave band.
Further, describedly excite optical filtering or excite filter coating to be single passage, two channels, triple channel or four-way; Described reception optical filtering or reception filter coating are single passage, two channels, triple channel or four-way.
Further, the shape of described light-guiding pillar is cylindrical, triangular prism or tetragonal body.
Further, described camera is CMOS camera or CCD camera.CCD and CMOS camera lens may be used to the fluorescence of DNA after PCR reacts and catches, and obtains graphic information, is supplied to Computer Analysis.
Accompanying drawing explanation
Below in conjunction with drawings and Examples, technical solutions of the utility model are further illustrated:
Fig. 1 is the schematic diagram that the utility model is applied to the side luminous optical detection system embodiment one of PCR instrument;
Fig. 2 is the vertical view of Fig. 1.
In figure: retaining plate 1, Reagent Tube 2, camera 3, LED 4, optics female seat 5, optics female seat extender 6, excite optical filtering 7, light-guiding pillar 8, guide-lighting groove 9, through hole 10, opening 11, receive optical filtering 12.
Embodiment
Embodiment one:
As Fig. 1, shown in Fig. 2, the utility model is applied to the side luminous optical detection system of PCR instrument, comprise retaining plate 1, exciting light system, transparent agent pipe 2 and camera 3, retaining plate 1 is horizontally disposed with, exciting light system comprises optics permanent seat, illuminator, light-guiding pillar 8 and guide-lighting groove, optics permanent seat comprises optics female seat 5 and optics female seat extender, optics female seat 5 is connected with bolt with the left side of retaining plate 1, illuminator is LED 4, LED 4 is positioned at optics female seat 5, light-guiding pillar 8 is cylindrical, light-guiding pillar 8 is positioned at optics female seat extender, LED 4 and light-guiding pillar 8 are all parallel with retaining plate 1, optics female seat 5 is connected by screw with optics female seat extender 6 and the end of the optics female seat 5 exciting optical filtering 7 to install to be connected in both, excite optical filtering 7 for single passage, permission wavelength is that the light of 470-505nm passes through, guide-lighting groove 9 is rectangular tank, the bottom of guide-lighting groove 9 is provided with opening 11, the top of guide-lighting groove 9 is connected with the Right vertical of retaining plate 1, the right side of retaining plate 1 is positioned at place directly over guide-lighting groove 9 and offers the hole holding Reagent Tube 2, the sidewall of guide-lighting groove 9 is provided with the through hole 10 with light-guiding pillar 8 vertical connection, Reagent Tube 2 is vertically placed on the side that in guide-lighting the groove 9 and bottom of Reagent Tube 2 is positioned at through hole 10, camera 3 is positioned at the below of guide-lighting groove 9, camera 3 is CMOS camera, be provided with between camera 3 and guide-lighting groove 9 and receive optical filtering 12, support is provided with above camera 3, receiving optical filtering 12 is fixed on support, receiving optical filtering 12 is single passage, and wavelength can be allowed to be that the light of 523-543nm passes through, light-guiding pillar 8 is communicated with through hole 10 and vertical.
In specific implementation process, operation steps is:
First: put into guide-lighting groove 9 by the hole of Reagent Tube 2 on the right side of retaining plate 1 that fluorescent reagent is housed, Reagent Tube 2 is glass material making, and guide-lighting groove 9 adopts aluminium to make;
Second: open LED 4, emit beam, through exciting optical filtering 7 to filter, be directed in the guide-lighting groove 9 of perpendicular connection through light-guiding pillar 8 by the through hole 10 of guide-lighting groove 9 sidewall, the exciting light of fluorescent samples in Reagent Tube 2 by importing into from its side through hole 10, send specific fluorescent, then the filtration passing perpendicularly through reception optical filtering 12 by guide-lighting groove 9 lower end through hole 10 by the clear bottom of Reagent Tube 2 is injected in camera 3;
3rd: camera 3 obtains graphic information, be supplied to Computer Analysis and carry out Data Data process, the fluorescence information that analyzing and testing obtains.
Embodiment two:
Be with the difference of embodiment one: by the reception optical filtering 12 of embodiment one with excite optical filtering 7 to replace with to receive filter coating and excite filter coating, excite filter coating and receive after filter coating is coated on transparent glass surface and install, the passage receiving filter coating is two channels, wavelength can be allowed to be that the light of 470-505nm wave band passes through, excite the passage of filter coating to be two channels, wavelength can be allowed to be that the light of 523-543nm wave band passes through.
Embodiment three:
With the difference of embodiment two be: the end of the optics female seat 5 exciting filter coating to install to be connected in both of embodiment two is replaced with: excite filter coating to be coated on end that glass surface installs the optics female seat extender be connected in both.
Embodiment four:
Be with the difference of embodiment two: the end of the optics female seat 5 exciting filter coating to install to be connected in both of embodiment two is replaced with: excite filter coating to install in light-guiding pillar 8.
Embodiment five:
Be with the difference of embodiment one: the LED 4 of embodiment one is replaced with halogen tungsten lamp.
Embodiment six:
Be with the difference of embodiment one: the LED 4 of embodiment one is replaced with halogen lamp.
Embodiment seven:
Be with the difference of embodiment one: be the cylindrical triangular prism that replaces with by the light-guiding pillar 8 of embodiment one, by the camera 3 of embodiment one for CMOS camera replaces with CCD camera.
Certainly, if the passage receiving optical filtering 12 or reception filter coating is triple channel, permission wavelength is that the light of wave band passes through; The passage exciting optical filtering or reception filter coating is triple channel, and permission wavelength is that the light of 470-505nm wave band passes through, or is four-way, and permission wavelength is that the light of 523-543nm wave band passes through, all within protection domain.
For a person skilled in the art; under the prerequisite not departing from the utility model structure; can also make some distortion and improvement, these also should be considered as protection domain of the present utility model, and these all can not affect effect and the practical applicability of the utility model enforcement.
Claims (10)
1. be applied to the side luminous optical detection system of PCR instrument, it is characterized in that: comprise retaining plate, light path system, transparent agent pipe and camera, retaining plate is horizontally disposed with;
Light path system comprises the guide-lighting groove of optics permanent seat, illuminator, light-guiding pillar and placement reagent, and optics permanent seat is connected with retaining plate, and illuminator and light-guiding pillar are all positioned at optics permanent seat, and illuminator and light-guiding pillar are all parallel with retaining plate;
The bottom of guide-lighting groove is provided with opening and guide-lighting groove is connected with retaining plate is vertical, and Reagent Tube is vertically positioned at guide-lighting groove, and camera is positioned at the below of guide-lighting groove, is provided with the first optical filtering between camera and guide-lighting groove;
The sidewall of guide-lighting groove is provided with the through hole with light-guiding pillar vertical connection, is provided with the second optical filtering between illuminator and light-guiding pillar through hole.
2. be applied to the side luminous optical detection system of PCR instrument as claimed in claim 1, it is characterized in that: the first described optical filtering replaces with the first filter coating; The second described optical filtering replaces with the second filter coating.
3. be applied to the side luminous optical detection system of PCR instrument as claimed in claim 1 or 2, it is characterized in that: the second described optical filtering or the second filter coating are between light source and light-guiding pillar or be positioned at light-guiding pillar.
4. be applied to the side luminous optical detection system of PCR instrument as claimed in claim 3, it is characterized in that: described optics permanent seat comprises optics female seat and optics female seat extender, illuminator is positioned at optics female seat, light-guiding pillar is positioned at optics female seat extender, optics female seat and optics female seat extended seat removably connect, and the second optical filtering or the second filter coating are between optics female seat and optics female seat extender.
5. be applied to the side luminous optical detection system of PCR instrument as claimed in claim 4, it is characterized in that: the second described optical filtering or the second filter coating are positioned at the end of optics female seat.
6. be applied to the side luminous optical detection system of PCR instrument as claimed in claim 4, it is characterized in that: the second described optical filtering or the second filter coating are positioned at the end of optics female seat extender.
7. as described in claim 5 or 6, be applied to the side luminous optical detection system of PCR instrument, it is characterized in that: described illuminator is LED, halogen tungsten lamp or halogen lamp.
8. be applied to the side luminous optical detection system of PCR instrument as claimed in claim 7, it is characterized in that: the second described optical filtering or the second filter coating are single passage, two channels, triple channel or four-way; The first described optical filtering or the first filter coating are single passage, two channels or triple channel.
9. be applied to the side luminous optical detection system of PCR instrument as claimed in claim 8, it is characterized in that: the shape of described light-guiding pillar is cylindrical, triangular prism or tetragonal body.
10. be applied to the side luminous optical detection system of PCR instrument as claimed in claim 9, it is characterized in that: described camera is CMOS camera or CCD camera.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420804189.5U CN204356338U (en) | 2014-12-18 | 2014-12-18 | Be applied to the side luminous optical detection system of PCR instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420804189.5U CN204356338U (en) | 2014-12-18 | 2014-12-18 | Be applied to the side luminous optical detection system of PCR instrument |
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| CN204356338U true CN204356338U (en) | 2015-05-27 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201420804189.5U Expired - Fee Related CN204356338U (en) | 2014-12-18 | 2014-12-18 | Be applied to the side luminous optical detection system of PCR instrument |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105400691A (en) * | 2015-12-11 | 2016-03-16 | 舟山医院 | A multifunctional PCR instrument |
| CN107513490A (en) * | 2017-09-29 | 2017-12-26 | 重庆京因生物科技有限责任公司 | A kind of full-automatic medical fluorescent PCR analysis system based on POCT patterns |
| CN112113938A (en) * | 2020-07-20 | 2020-12-22 | 北京理工大学 | Multi-channel gaseous substance detection device |
-
2014
- 2014-12-18 CN CN201420804189.5U patent/CN204356338U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105400691A (en) * | 2015-12-11 | 2016-03-16 | 舟山医院 | A multifunctional PCR instrument |
| CN107513490A (en) * | 2017-09-29 | 2017-12-26 | 重庆京因生物科技有限责任公司 | A kind of full-automatic medical fluorescent PCR analysis system based on POCT patterns |
| CN112113938A (en) * | 2020-07-20 | 2020-12-22 | 北京理工大学 | Multi-channel gaseous substance detection device |
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| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150527 Termination date: 20151218 |
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| EXPY | Termination of patent right or utility model |