CN211877770U - Double excitation light source structure for biological sample fluorescence detection - Google Patents
Double excitation light source structure for biological sample fluorescence detection Download PDFInfo
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- CN211877770U CN211877770U CN202020513841.3U CN202020513841U CN211877770U CN 211877770 U CN211877770 U CN 211877770U CN 202020513841 U CN202020513841 U CN 202020513841U CN 211877770 U CN211877770 U CN 211877770U
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- 230000005284 excitation Effects 0.000 title claims abstract description 52
- 238000001917 fluorescence detection Methods 0.000 title claims abstract description 20
- 239000012472 biological sample Substances 0.000 title claims abstract description 12
- 238000012634 optical imaging Methods 0.000 claims abstract description 35
- 239000011521 glass Substances 0.000 claims abstract description 18
- 238000010186 staining Methods 0.000 claims abstract description 8
- 230000009977 dual effect Effects 0.000 claims description 13
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 claims description 8
- 230000003760 hair shine Effects 0.000 claims description 6
- 239000000523 sample Substances 0.000 claims description 4
- 238000004519 manufacturing process Methods 0.000 abstract description 15
- 238000001514 detection method Methods 0.000 abstract description 12
- 235000012431 wafers Nutrition 0.000 abstract 1
- 230000001575 pathological effect Effects 0.000 description 6
- 238000010586 diagram Methods 0.000 description 2
- 238000004043 dyeing Methods 0.000 description 2
- 230000002349 favourable effect Effects 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000003759 clinical diagnosis Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000008506 pathogenesis Effects 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
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Abstract
The utility model discloses a double excitation light source structure for fluorescence detection of biological sample wafers, which comprises a spectacle frame component, a first lens, a second lens, a first light source component and a second light source component; the upper end part of the mirror bracket assembly is connected with a biological optical imaging system, and a glass slide carrying a staining specimen is arranged below the mirror bracket assembly; the first lens and the second lens are both connected in the frame assembly; light rays emitted by the first light source assembly irradiate on the first lens and are reflected to form first irradiation light rays, the first irradiation light rays irradiate on the glass slide and excite the stained specimen to emit first excitation light rays, and the first excitation light rays sequentially penetrate through the first lens and the second lens and then enter the biological optical imaging system. The utility model discloses can satisfy biological optical imaging system to the demand of two kinds of light sources to simple structure, occupation space is little, can reduce medical detection equipment's the manufacturing cost and the manufacturing degree of difficulty.
Description
Technical Field
The utility model relates to a two excitation light source structures for biological sample piece fluorescence detection.
Background
At present, biological sample fluorescence detection is a means for staining a biological sample specimen by fluorescein, irradiating the stained specimen by an excitation light source, and detecting the specimen by using a biological optical imaging technology, and has wide application prospects in the aspects of researching pathogenesis, clinical diagnosis and the like. With the increase of population and the rapid development of industry, the detection and research business of hospitals is heavier and heavier, so that two pathological specimens are likely to be detected on the same glass slide during fluorescence detection, and two different excitation light sources are needed for exciting fluorescein of the two pathological specimens; sometimes, the same pathological specimen needs two excitation light sources to excite fluorescein to realize biological optical imaging.
At present, the existing solutions are to obtain the light of different wave bands by switching a plurality of excitation light sources of different wave bands, or to obtain the light of different wave bands by switching a plurality of optical filters by using a single excitation light source. However, the above two methods both require a switching mechanism, and the switching mechanism inevitably causes a shortage of space in the medical examination apparatus, increases cost and manufacturing difficulty, is not suitable for mass production of the medical examination apparatus, and does not conform to the tendency of the medical examination apparatus to be domesticated.
Disclosure of Invention
The utility model aims to solve the technical problem that overcome prior art's defect, provide a two excitation light source structures for biological appearance piece fluorescence detection, it can satisfy biological optical imaging system to the demand of two kinds of light sources to simple structure, occupation space is little, can reduce medical check out test set's manufacturing cost and the manufacturing degree of difficulty.
In order to solve the technical problem, the technical scheme of the utility model is that: a dual excitation light source structure for fluorescence detection of biological samples, comprising:
the upper end part of the spectacle frame assembly is used for connecting a biological optical imaging system, and a glass slide carrying a staining specimen is arranged below the spectacle frame assembly;
a first lens and a second lens coupled in the frame assembly;
the first light source assembly is connected to the mirror bracket assembly, light emitted by the first light source assembly irradiates the first lens and is reflected to form first irradiation light, the first irradiation light irradiates the glass slide and excites the stained specimen to emit first excitation light, and the first excitation light sequentially penetrates through the first lens and the second lens and then enters the biological optical imaging system;
the connection is in second light source subassembly on the mirror holder subassembly, the light that the second light source subassembly sent shines form the second after being reflected on the second lens, the second shines light and sees through shine behind the first lens on the slide glass and arouse the staining sample sends the second excitation light, the second excitation light sees through in proper order first lens with get into behind the second lens biological optical imaging system.
Further provides a specific scheme of the spectacle frame assembly, wherein the spectacle frame assembly comprises a spectacle frame, a first spectacle tube and a second spectacle tube; wherein,
the first lens and the second lens are both connected in the frame;
the first mirror tube is connected to the mirror frame and provided with a first light channel;
the first light source assembly is connected to the first lens tube, and light rays emitted by the first light source assembly penetrate through the first light channel and then irradiate on the first lens;
the second mirror tube is connected to the mirror frame and is provided with a second light channel;
the second light source assembly is connected to the second lens tube, and light rays emitted by the second light source assembly penetrate through the second light channel and then irradiate on the second lens.
Further, be equipped with the main entrance in the picture frame, first lens with the second lens is from up locating in proper order down in the main entrance, the upper end of main entrance with biological optical imaging system links to each other.
Further, the included angle between the first lens and the vertical direction and the included angle between the second lens and the vertical direction are both 45 degrees, and the included angle between the first lens and the second lens is 90 degrees.
Further provides a concrete scheme of the first light source assembly, the first light source assembly includes a first light source and a first radiator, the first light source is connected to the first radiator, and the first radiator is connected to the first mirror tube.
The second light source assembly comprises a second light source and a second radiator, the second light source is connected to the second radiator, and the second radiator is connected to the second mirror tube.
Further, an objective lens is arranged between the lens frame assembly and the glass slide.
Further, the slide is located at a focal plane of the objective lens.
Further, the stained specimen is stained with fluorescein.
After the technical scheme is adopted, light emitted by the first light source assembly irradiates on the first lens and is reflected to form first irradiation light, the first irradiation light irradiates on the glass slide and excites the dyed specimen to emit first excitation light, and the first excitation light sequentially penetrates through the first lens and the second lens and then enters the biological optical imaging system to realize biological optical imaging. Light that the second light source subassembly sent shines and is formed the second after being reflected on the second lens and shine light, the second shines light and sees through shine behind the first lens on the slide glass and arouse the dyeing sample sends second excitation light, second excitation light sees through in proper order first lens with get into behind the second lens bio-optical imaging system to realize bio-optical imaging, thereby satisfied bio-optical imaging system is to the demand of two kinds of light sources, and simple structure, occupation space is little, and then has reduced medical detection equipment's manufacturing cost and the manufacturing degree of difficulty, is favorable to medical detection equipment's volume production, accords with the trend of medical detection equipment family ization.
Drawings
FIG. 1 is a schematic diagram of the internal structure of a dual excitation light source structure for fluorescence detection of biological specimens according to the present invention;
fig. 2 is a schematic diagram of the external structure of the dual excitation light source structure for fluorescence detection of biological samples according to the present invention.
Detailed Description
In order that the present invention may be more readily and clearly understood, the following detailed description of the present invention is provided in connection with the accompanying drawings.
As shown in fig. 1 and 2, a dual excitation light source structure for fluorescence detection of a biological sample, comprising:
the upper end part of the spectacle frame assembly is used for connecting a biological optical imaging system 1, and a glass slide 2 carrying a staining specimen is arranged below the spectacle frame assembly;
a first lens 3 and a second lens 4 attached in the frame assembly;
the first light source assembly is connected to the mirror bracket assembly, light emitted by the first light source assembly irradiates the first lens 3 and is reflected to form first irradiation light 5, the first irradiation light 5 irradiates the glass slide 2 and excites the stained specimen to emit first excitation light 6, and the first excitation light 6 sequentially penetrates through the first lens 3 and the second lens 4 and then enters the biological optical imaging system 1 to realize biological optical imaging;
the second light source assembly is connected to the spectacle frame assembly, light emitted by the second light source assembly irradiates the second lens 4 and is reflected to form second irradiation light 7, the second irradiation light 7 irradiates the glass slide 2 after penetrating through the first lens 3 and excites the stained specimen to emit second excitation light 8, and the second excitation light 8 sequentially penetrates through the first lens 3 and the second lens 4 and enters the biological optical imaging system 1 to realize biological optical imaging, so that the requirements of the biological optical imaging system 1 on two light sources are met, the structure is simple, the occupied space is small, the production cost and the manufacturing difficulty of medical detection equipment are reduced, the mass production of the medical detection equipment is facilitated, and the family trend of the medical detection equipment is met; specifically, the bio-optical imaging system 1 is the prior art, and details thereof are not described in this embodiment.
As shown in fig. 1 and 2, the frame assembly includes, for example, but not limited to, a frame 9, a first lens tube 10, and a second lens tube 11; wherein,
the first lens 3 and the second lens 4 are both connected in the frame 9;
the first lens tube 10 is connected to the lens frame 9 and is provided with a first light channel 12;
the first light source assembly is connected to the first lens tube 10, and light emitted by the first light source assembly passes through the first light channel 12 and then irradiates the first lens 3;
the second lens tube 11 is connected to the lens frame 9 and is provided with a second light channel 13;
the second light source assembly is connected to the second lens tube 11, and light emitted by the second light source assembly passes through the second light channel 13 and then irradiates the second lens 4.
As shown in fig. 1 and 2, the frame 9 may be provided with a main channel 14, the first lens 3 and the second lens 4 are sequentially disposed in the main channel 14 from bottom to top, and an upper end of the main channel 14 is connected to the bio-optical imaging system 1.
Specifically, the included angle between the first lens 3 and the vertical direction and the included angle between the second lens 4 and the vertical direction can both be 45 °, and the included angle between the first lens 3 and the second lens 4 can be 90 °.
As shown in fig. 1 and 2, the first light source assembly includes, for example and without limitation, a first light source 15 and a first heat sink 16, the first light source 15 is connected to the first heat sink 16, and the first heat sink 16 is connected to the first mirror tube 10.
The second light source assembly includes, for example and without limitation, a second light source 17 and a second heat sink 18, the second light source 17 is connected to the second heat sink 18, and the second heat sink 18 is connected to the second mirror tube 11.
As shown in fig. 1 and 2, an objective lens 19 is further arranged between the lens frame assembly and the slide 2; specifically, the first irradiation light 5 and the second irradiation light 7 irradiate on the glass slide 2 after passing through the objective lens 19, and the first excitation light 6 and the second excitation light 8 sequentially pass through the first lens 3 and the second lens 4 after passing through the objective lens 19.
In particular, the slide 2 is located in the focal plane of the objective lens 19.
In this embodiment, the stained specimen is stained by fluorescein, and the specimen may be a pathological specimen; specifically, the first light source 15 and the second light source 17 with corresponding wave bands are selected according to the requirement of fluorescein on the light source used during the pathological specimen staining, the wave band of the first light source 15 is different from that of the second light source 17, and the first excitation light 6 and the second excitation light 8 are both emitted by the excitation of fluorescein. The first excitation light rays 6 and the second excitation light rays 8 have certain identification degrees after entering the biological optical imaging system 1, can be matched with the biological optical imaging system 1, and can meet the requirements of fluorescence optical imaging detection of pathological specimens.
The working principle of the utility model is as follows:
light rays emitted by the first light source assembly irradiate on the first lens 3 and are reflected to form first irradiation light rays 5, the first irradiation light rays 5 irradiate on the glass slide 2 and excite the dyed specimen to emit first excitation light rays 6, and the first excitation light rays 6 sequentially penetrate through the first lens 3 and the second lens 4 and then enter the biological optical imaging system 1 to achieve biological optical imaging. Light that the second light source subassembly sent shines on the second lens 4 and by formation second irradiation light 7 after the reflection, second irradiation light 7 sees through shine behind the first lens 3 on the slide glass 2 and arouse the dyeing sample sends second excitation light 8, second excitation light 8 sees through in proper order first lens 3 with enter behind the second lens 4 bio-optical imaging system 1 to realize bio-optical imaging, thereby satisfied bio-optical imaging system 1 is to the demand of two kinds of light sources, and simple structure, occupation space is little, and then has reduced medical detection equipment's manufacturing cost and the manufacturing degree of difficulty, is favorable to medical detection equipment's volume production, accords with the trend of medical detection equipment family.
The above-mentioned embodiments further explain in detail the technical problems, technical solutions and advantages solved by the present invention, and it should be understood that the above only is a specific embodiment of the present invention, and is not intended to limit the present invention, and any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the scope of the present invention.
In the description of the present invention, it is to be understood that the terms indicating orientation or positional relationship are based on the orientation or positional relationship shown in the drawings, and are only for convenience of description and simplification of description, and do not indicate or imply that the equipment or elements referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.
In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," and "fixed" are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate the position or positional relationship based on the position or positional relationship shown in the drawings, or the position or positional relationship which is usually placed when the product of the present invention is used, and are only for convenience of description and simplification of the description, but do not indicate or imply that the device or element referred to must have a specific position, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," "third," and the like are used solely to distinguish one from another and are not to be construed as indicating or implying relative importance.
Furthermore, the terms "horizontal", "vertical", "overhang" and the like do not imply that the components are required to be absolutely horizontal or overhang, but may be slightly inclined. For example, "horizontal" merely means that the direction is more horizontal than "vertical" and does not mean that the structure must be perfectly horizontal, but may be slightly inclined.
In the present disclosure, unless otherwise expressly stated or limited, the first feature may comprise both the first and second features directly contacting each other, and also may comprise the first and second features not being directly contacting each other but being in contact with each other by means of further features between them. Also, the first feature being above, on or above the second feature includes the first feature being directly above and obliquely above the second feature, or merely means that the first feature is at a higher level than the second feature. A first feature that underlies, and underlies a second feature includes a first feature that is directly under and obliquely under a second feature, or simply means that the first feature is at a lesser level than the second feature.
Claims (9)
1. A dual excitation light source structure for fluorescence detection of biological samples, comprising:
the upper end part of the spectacle frame assembly is used for connecting a biological optical imaging system (1), and a glass slide (2) carrying a staining specimen is arranged below the spectacle frame assembly;
a first lens (3) and a second lens (4) connected in the frame assembly;
the first light source assembly is connected to the spectacle frame assembly, light emitted by the first light source assembly irradiates the first lens (3) and is reflected to form first irradiation light (5), the first irradiation light (5) irradiates the glass slide (2) and excites the stained specimen to emit first excitation light (6), and the first excitation light (6) sequentially penetrates through the first lens (3) and the second lens (4) and then enters the biological optical imaging system (1);
connect the second light source subassembly on the mirror holder subassembly, the light that the second light source subassembly sent shines form second irradiation light (7) on second lens (4) and after being reflected, second irradiation light (7) see through shine behind first lens (3) on slide glass (2) and arouse the staining sample sends second excitation light (8), second excitation light (8) see through in proper order first lens (3) with enter into behind second lens (4) biological optical imaging system (1).
2. The structure of dual excitation light sources for fluorescence detection of biological specimens according to claim 1, wherein the frame assembly comprises a frame (9), a first tube (10) and a second tube (11); wherein,
the first lens (3) and the second lens (4) are both connected in the frame (9);
the first lens tube (10) is connected to the lens frame (9) and is provided with a first light channel (12);
the first light source assembly is connected to the first lens tube (10), and light rays emitted by the first light source assembly penetrate through the first light channel (12) and then irradiate on the first lens (3);
the second lens tube (11) is connected to the lens frame (9) and is provided with a second light channel (13);
the second light source assembly is connected to the second lens tube (11), and light rays emitted by the second light source assembly penetrate through the second light channel (13) and then irradiate the second lens (4).
3. The structure of dual excitation light sources for fluorescence detection of biological samples according to claim 2, wherein the frame (9) has a main channel (14), the first lens (3) and the second lens (4) are sequentially disposed in the main channel (14) from bottom to top, and an upper end of the main channel (14) is connected to the bio-optical imaging system (1).
4. The structure of dual excitation light sources for fluorescence detection of biological samples according to claim 3, wherein the included angle between the first lens (3) and the vertical direction and the included angle between the second lens (4) and the vertical direction are both 45 °, and the included angle between the first lens (3) and the second lens (4) is 90 °.
5. The structure of dual excitation light sources for fluorescence detection of biological specimens as claimed in claim 2, wherein the first light source assembly comprises a first light source (15) and a first heat sink (16), the first light source (15) is connected to the first heat sink (16), and the first heat sink (16) is connected to the first mirror tube (10).
6. The structure of dual excitation light sources for fluorescence detection of biological specimens as claimed in claim 2, wherein the second light source assembly comprises a second light source (17) and a second heat sink (18), the second light source (17) is connected to the second heat sink (18), and the second heat sink (18) is connected to the second mirror tube (11).
7. The structure of dual excitation light sources for fluorescence detection of biological specimens according to claim 1, wherein an objective lens (19) is further disposed between the frame assembly and the slide (2).
8. The structure of dual excitation light sources for fluorescence detection of biological specimens according to claim 7, wherein the slide (2) is located on the focal plane of the objective lens (19).
9. The structure of dual excitation light sources for fluorescence detection of biological specimens according to claim 1, wherein the stained specimen is stained with fluorescein.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113933496A (en) * | 2021-07-19 | 2022-01-14 | 安徽桐康医疗科技股份有限公司 | A calibrating device for fluorescence immunoassay quantitative analysis appearance |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113933496A (en) * | 2021-07-19 | 2022-01-14 | 安徽桐康医疗科技股份有限公司 | A calibrating device for fluorescence immunoassay quantitative analysis appearance |
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