CN204318970U - Get egg apparatus - Google Patents

Get egg apparatus Download PDF

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Publication number
CN204318970U
CN204318970U CN201420492832.5U CN201420492832U CN204318970U CN 204318970 U CN204318970 U CN 204318970U CN 201420492832 U CN201420492832 U CN 201420492832U CN 204318970 U CN204318970 U CN 204318970U
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China
Prior art keywords
collection tube
embryo
ovum
germ cell
egg apparatus
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CN201420492832.5U
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Chinese (zh)
Inventor
肖丽萍
刘欢
刘楚新
李琴
张兴举
王俊
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BGI Shenzhen Co Ltd
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BGI Shenzhen Co Ltd
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Priority to CN201420492832.5U priority Critical patent/CN204318970U/en
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Abstract

This utility model belongs to biomedical auxiliary appliance technical field, provide one and get egg apparatus, for extracting ovum, germ cell and embryo from fallopian tube, it comprises: for temporary reservoir ovum, germ cell and the collection tube of embryo and the air pump for promoting and reduce collection tube internal gas pressure, collection tube is provided with upper orifice and the lower nozzle for sucking and discharge ovum, germ cell and embryo, lower nozzle is provided with for cutting open oviducal bayonet, air pump is provided with interface, and upper orifice is connected with interface.Filter is provided with between the upper orifice of collection tube and the interface of air pump.Bayonet becomes 30 ° with the axis of collection tube.This utility model by arranging bayonet on the lower nozzle of collection tube, can rapidly ovum, germ cell or embryo be sucked in collection tube after fallopian tube is cut open by bayonet, simplify operating process, shorten the operating time, damage can be reduced and pollute possibility of ovum, germ cell and embryo, effectively their quality of raising and survival rate.

Description

Get egg apparatus
Technical field
This utility model belongs to biomedical auxiliary appliance technical field, particularly relates to one and gets egg apparatus.
Background technology
At biological, medical domain, often need to extract intratubal ovum, germ cell and embryo, then they are carried out to next step operation (such as genetic modification).Current extraction ovum, germ cell and embryo mainly adopt following methods: scratch magnum tubae uterinae with syringe needle, and ovum, germ cell and embryo are flowed out, then collect with mouth suction pipe or pipettor, they are transferred in culture dish.Said method inconvenient operation, and the operating time is long, and easily damage and pollution ovum, germ cell and embryo, affect their quality and survival rate.
Mice is subjects important in life science, and wherein, the genetic modification of mice has become basic technology important in life sciences, for gene function and pathogenic mechanism research serve great facilitation.In mouse genetic modification, the ovum of collection mice or germ cell is often needed to carry out embryo operation.The embryo of mice or collecting fertilized eggs, in female Mus of superovulation, need to scratch magnum tubae uterinae with sharp weapon, transfer to the granular cell in hyaluronidase dropping liquid outside digestion parcel embryo again after allowing agglomerating embryo flow out with mouth suction pipe or pipettor.Said extracted method inconvenient operation, and the operating time is long, and easily damage and pollution ovum, germ cell and embryo, affect their quality and survival rate.
Utility model content
The purpose of this utility model is the defect overcoming prior art, provide a kind of effectively can improve ovum, germ cell and embryo quality and survival rate get egg apparatus.
This utility model realizes like this, one gets egg apparatus, for extracting ovum, germ cell and embryo from fallopian tube, it comprises: for temporary reservoir ovum, germ cell and the collection tube of embryo and the air pump for promoting and reduce described collection tube internal gas pressure, described collection tube is provided with upper orifice and the lower nozzle for sucking and discharge ovum, germ cell and embryo, described lower nozzle is provided with for cutting open oviducal bayonet, described air pump is provided with interface, and described upper orifice is connected with described interface.
Further, filter is provided with between the upper orifice of described collection tube and the interface of described air pump.
Further, described filter is provided with multiple filtering holes, and the aperture of described filtering holes is 0.22 μm.
Preferably, described filter and described collection tube one-body molded.
Particularly, described collection tube is cone shell shape.
More specifically, described bayonet becomes 30 ° with the axis of described collection tube.
Further, described bayonet and described collection tube one-body molded.
Further, described air pump comprises sleeve, the activity with movable chamber and is located at the piston of described movable intracavity and the push-pull bar for piston described in push-and-pull, described interface is located at described sleeve lower end, described sleeve upper end is provided with upper port, described push-pull bar one end is connected with described piston, and the other end stretches out outside described upper port.
Particularly, described sleeve lateral wall is provided with strip through hole, and described push-pull bar sidewall is provided with can the push-pull handle of slippage vertically in described strip through hole, and described push-pull handle is arranged on described strip through hole.
More specifically, the other end of described push-pull bar is provided with pressure cap.
Egg apparatus of getting provided by the utility model has following technique effect:
This utility model by arranging bayonet on the lower nozzle of collection tube, can rapidly ovum, germ cell or embryo be sucked in collection tube after fallopian tube is cut open by bayonet, simplify operating process, shorten the operating time, damage can be reduced and pollute possibility of ovum, germ cell and embryo, effectively their quality of raising and survival rate.In addition, because the processing technique of bayonet is simply easy, therefore, also manufacturing cost of the present utility model can effectively be reduced.
Accompanying drawing explanation
Fig. 1 is the longitudinal sectional view getting egg apparatus in this utility model embodiment, and wherein piston and push-pull bar are not analysed and observe.
Detailed description of the invention
In order to make the purpose of this utility model, technical scheme and advantage clearly understand, below in conjunction with drawings and Examples, this utility model is further elaborated.Should be appreciated that specific embodiment described herein only in order to explain this utility model, and be not used in restriction this utility model.
See Fig. 1, this utility model embodiment provides one and gets egg apparatus, for extracting ovum, germ cell and embryo from fallopian tube, it comprises: for temporary reservoir ovum, germ cell and the collection tube 1 of embryo and the air pump 2 for promoting and reduce described collection tube 1 internal gas pressure, described collection tube 1 is provided with upper orifice 11 and the lower nozzle 12 for sucking and discharge ovum, germ cell and embryo, described lower nozzle 12 is provided with for cutting open oviducal bayonet 13, described air pump 2 is provided with interface 212, and described upper orifice 11 is connected with described interface 212.
Extract ovum, germ cell or embryo time, first cut open fallopian tube with bayonet 13, then reduced rapidly the air pressure in collection tube 1 by air pump 2, by ovum, germ cell or embryo from lower nozzle 12 rapidly suction collection tube 1, complete extraction work.Egg apparatus is got in transfer, its lower nozzle 12 is aimed at object container or position, promotes the air pressure in collection tube 1 by air pump 2, ovum, germ cell or embryo are extruded from lower nozzle 12, complete release work.After the collection tube 1 more renewed, i.e. above-mentioned steps capable of circulation, what complete next round gets ovum work.
This utility model by arranging bayonet 13 on the lower nozzle 12 of collection tube 1, can rapidly ovum, germ cell or embryo be sucked in collection tube 1 after fallopian tube is cut open by bayonet 13, simplify operating process, shorten the operating time, damage can be reduced and pollute possibility of ovum, germ cell and embryo, effectively their quality of raising and survival rate.In addition, because the processing technique of bayonet 13 is simply easy, therefore, also manufacturing cost of the present utility model can effectively be reduced.
The gaseous contamination collection tube 1 be blown into for avoiding air pump 2, avoids the ovum of suction in collection tube 1, germ cell or embryo to flow in air pump 2 simultaneously, is provided with filter 3 between the upper orifice 11 of described collection tube and the interface 212 of described air pump.
Described filter 3 is provided with multiple filtering holes, and the aperture of described filtering holes is 0.22 μm.The aperture of 0.22 μm, both can prevent the ovum in collection tube 1, germ cell or embryo from flowing in air pump 2, the gas again in tolerable air pump 2 flows in collection tube 1.Certainly, this utility model can also adopt the filter of other structures to realize above-mentioned functions, and it is not limited with the present embodiment.
As a kind of embodiment of the present utility model, described filter 3 is one-body molded with described collection tube 1, and operator are that an entirety is changed with collection tube 1 and filter 3, greatly can simplify their replacing program.
Described collection tube 1 is in cone shell shape.The inner chamber of collection tube 1 is conical cavity, is conducive to ovum, the suction of germ cell and embryo, discharge and storage.
In the present embodiment, described bayonet 13 becomes 30 ° with the axis of described collection tube 1, and bayonet 13, with this vibrational power flow, is more convenient for puncturing and is cut open fallopian tube, is easier to ovum, germ cell and embryo to suck in collection tube 1.
Described bayonet 13 can be one-body molded with described collection tube 1, also can be installed on collection tube 1 in sheathed mode, its its concrete condition visual and manufacturing.Bayonet 13 of the present utility model and collection tube 1 can be identical material (such as plastics), and they can be one-body molded like this.
As a kind of detailed description of the invention of this utility model about air pump 2, described air pump 2 comprises sleeve 21, the activity with movable chamber 211 and is located at the piston 22 in described movable chamber 211 and the push-pull bar 23 for piston described in push-and-pull 22, described interface 212 is located at described sleeve 21 lower end, described sleeve 21 upper end is provided with upper port 213, described push-pull bar 23 one end is connected with described piston 22, and the other end stretches out outside described upper port 213.When upwards pulling piston 22, the air pressure in movable chamber 211 can be reduced, and then drive collection tube 1 to suck ovum, germ cell or embryo.During downward promotion piston 22, the air pressure in movable chamber 211 can be promoted, and then drive collection tube 1 to discharge ovum, germ cell or embryo.
Particularly, described sleeve 21 sidewall is provided with strip through hole 214, and described push-pull bar 23 sidewall is provided with can the push-pull handle 231 of slippage vertically in described strip through hole 214, and described push-pull handle 231 is arranged on described strip through hole 214.Operator, by one-handed performance push-pull handle 231, just can complete and operate the push-and-pull of piston 22.
The other end of described push-pull bar 23 is provided with pressure cap 232, adds pressure cap 232, can strengthen the contact area between push-pull bar 23 and hands, reduces the pressure of opponent.
It should be noted that, the air pump 2 in this utility model is not limited to the form that the present embodiment provides, and it can also be that other can promote and reduce the appliance arrangement of collection tube 1 internal gas pressure, if needed, it can also be the electric air pump of automatization's type.
What the present embodiment provided comprises the following steps when getting egg apparatus use:
The uterine part that the ovary fallopian tube taken off in Mice Body connects is placed in operation liquid;
Find oviducal portion of expanding under the microscope;
Cut open magnum tubae uterinae with bayonet 13 of the present utility model, ovum, germ cell or embryo are flowed out thereupon;
Upwards slippage push-pull handle 231, makes piston 22 move up, and ovum, germ cell or embryo is sucked rapidly in collection tube 1;
After ovum, germ cell or embryo extract, egg apparatus is got in transfer, is inserted by collection tube 1 and is equipped with in the container of hyaluronidase;
The pressure cap 232 of pressing push-pull bar 23 upper end, makes piston 22 slippage downwards, the ovum in collection tube 1, germ cell or embryo is discharged;
Above step can complete extraction to ovum, germ cell or embryo and release/discharge.The collection tube 1 more renewed, circulation above-mentioned steps, can carry out extraction and the releasing operation of next round.
The foregoing is only preferred embodiment of the present utility model; not in order to limit this utility model; all do within spirit of the present utility model and principle any amendment, equivalent to replace or improvement etc., all should be included within protection domain of the present utility model.

Claims (10)

1. get egg apparatus for one kind, for extracting ovum, germ cell and embryo from fallopian tube, it is characterized in that, comprise: for temporary reservoir ovum, germ cell and the collection tube of embryo and the air pump for promoting and reduce described collection tube internal gas pressure, described collection tube is provided with upper orifice and the lower nozzle for sucking and discharge ovum, germ cell and embryo, described lower nozzle is provided with for cutting open oviducal bayonet, and described air pump is provided with interface, and described upper orifice is connected with described interface.
2. get egg apparatus as claimed in claim 1, it is characterized in that: between the upper orifice of described collection tube and the interface of described air pump, be provided with filter.
3. get egg apparatus as claimed in claim 2, it is characterized in that: described filter is provided with multiple filtering holes, the aperture of described filtering holes is 0.22 μm.
4. get egg apparatus as claimed in claim 2, it is characterized in that: described filter and described collection tube one-body molded.
5. get egg apparatus as claimed in claim 1, it is characterized in that: described collection tube is cone shell shape.
6. get egg apparatus as claimed in claim 1, it is characterized in that: described bayonet becomes 30 ° with the axis of described collection tube.
7. get egg apparatus as claimed in claim 1, it is characterized in that: described bayonet and described collection tube one-body molded.
8. as described in any one of claim 1-7, get egg apparatus, it is characterized in that: described air pump comprises sleeve, the activity with movable chamber and is located at the piston of described movable intracavity and the push-pull bar for piston described in push-and-pull, described interface is located at described sleeve lower end, described sleeve upper end is provided with upper port, described push-pull bar one end is connected with described piston, and the other end stretches out outside described upper port.
9. get egg apparatus as claimed in claim 8, it is characterized in that: described sleeve lateral wall is provided with strip through hole, described push-pull bar sidewall is provided with can the push-pull handle of slippage vertically in described strip through hole, and described push-pull handle is arranged on described strip through hole.
10. get egg apparatus as claimed in claim 8, it is characterized in that: the other end of described push-pull bar is provided with pressure cap.
CN201420492832.5U 2014-08-29 2014-08-29 Get egg apparatus Active CN204318970U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201420492832.5U CN204318970U (en) 2014-08-29 2014-08-29 Get egg apparatus

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201420492832.5U CN204318970U (en) 2014-08-29 2014-08-29 Get egg apparatus

Publications (1)

Publication Number Publication Date
CN204318970U true CN204318970U (en) 2015-05-13

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN201420492832.5U Active CN204318970U (en) 2014-08-29 2014-08-29 Get egg apparatus

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CN (1) CN204318970U (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105219631A (en) * 2015-10-28 2016-01-06 汤占宇 A kind of cell harvesting pin
CN107794213A (en) * 2017-11-30 2018-03-13 南昌大学 A kind of electronic ovarian follicle revulseur

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105219631A (en) * 2015-10-28 2016-01-06 汤占宇 A kind of cell harvesting pin
CN107794213A (en) * 2017-11-30 2018-03-13 南昌大学 A kind of electronic ovarian follicle revulseur

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C14 Grant of patent or utility model
GR01 Patent grant
CP01 Change in the name or title of a patent holder

Address after: 518083 comprehensive building, Beishan Industrial Zone, Yantian District, Guangdong, Shenzhen

Patentee after: BGI SHENZHEN

Patentee after: Shenzhen Huada Gene Technology Co., Ltd.

Address before: 518083 comprehensive building, Beishan Industrial Zone, Yantian District, Guangdong, Shenzhen

Patentee before: BGI SHENZHEN

Patentee before: Shenzhen Huada Gene Technology Co., Ltd.

CP01 Change in the name or title of a patent holder