CN107794213A - A kind of electronic ovarian follicle revulseur - Google Patents
A kind of electronic ovarian follicle revulseur Download PDFInfo
- Publication number
- CN107794213A CN107794213A CN201711235468.9A CN201711235468A CN107794213A CN 107794213 A CN107794213 A CN 107794213A CN 201711235468 A CN201711235468 A CN 201711235468A CN 107794213 A CN107794213 A CN 107794213A
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- Prior art keywords
- revulseur
- ovarian follicle
- electronic
- puncture channel
- guide rod
- Prior art date
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/06—Tubular
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
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- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Cell Biology (AREA)
- Clinical Laboratory Science (AREA)
- Infusion, Injection, And Reservoir Apparatuses (AREA)
Abstract
The present invention relates to technical field of cell biology, more particularly to a kind of electronic ovarian follicle revulseur.The electronic ovarian follicle revulseur of kind, including fuselage support, the inside of the fuselage support is provided with puncture channel, matched guide rod is provided with the puncture channel, one end of the guide rod moves back and forth via puncture channel described in electric drive unit drives edge, and the other end stretches out the puncture channel and is connected with asepsis injector syringe needle.Whole device greatly reduces the work load of experimenter, has been obviously improved conventional efficient by the way that simply cleverly design improves the deficiency that existing ovarian follicle needle point method obtains granular cell.
Description
Technical field
The present invention relates to technical field of cell biology, more particularly to a kind of electronic ovarian follicle revulseur.
Background technology
Ovary is the Main Reproductive Organs of female mammal, and ovarian follicle is the main constituents in ovary.Primordial follicle
It is made up of the individual layer scales granular cell of egg mother cell and surrounding, granular cell participates in regulation and control ovarian follicle from growing maturation
The whole process of ovulation, they will undergo primary, secondary follicle and antral follicles stage successively, be ovulated subsequently into graaffian follicle
Phase.From primordial follicle to Graafian follicle or atretic follicle is a complicated and accurate process, the granular cell of differentiation with
Egg mother cell, which is formed, closely to be combined and provides the exchange of information substance, while granular cell can secrete progesterone, estrogen etc.
Hormone and expression specificity acceptor(FSHR), for example ovulated with this to coordinate the ripe and subsequent activity of Oocyte Development,
Promote fertilization and early embryonic development etc..Granular cell forms entirety functionally with egg mother cell, when granular cell withers
The locking of ovarian follicle will be caused by dying, ovarian follicle once locking can not normal development, ultimately result in female reproduction function associated disorders
With disease.
Primary gonad granulocyte in-vitro culture model is established, is easy to carry out granular cell growth under in vitro conditions, is increased
Grow, differentiation and the research of apoptosis, provided to study the researchs such as female Ovarian Physiological Function, ovary relevant disease and Reproductive toxicology
Good means.The gene of mouse is highly similar to the mankind, and its all kinds of biochemical reactions and people are basically identical, and have and be easy to
Feeding cost is low to wait many merits, therefore is the optimal selection of experimental animal.First, the ovarian granulosa for obtaining a large amount of high quality is thin
Born of the same parents are the important foundations of this aspect research.Mouse granulosa cells separation is mostly shown using experimenter stereoscopic in document at present
Stainless steel punctures ovarian follicle is used under micro mirror, granular cell is discharged into the method in culture medium [1-4].This method is turned out
The primary granular cell purity come is high, and growth and proliferation of cell ability is strong.But there is also weak point, first experimenter to hold
Stainless pin scalp acupuncture pierces ovarian follicle, and efficiency very lowly takes time and effort, and difficult to realize be needled into each ovarian follicle and make granular cell
Thoroughly release, this will largely reduce the collecting amount of granular cell.And if experimenter is in order to collect more granular cells
And abundant acupuncture ovarian follicle, it can generally take again long, granular cell as being transferred in the environment of suitable growth not in time, the death rate
It can increase.Meanwhile the operating time is long, cell is easier contaminated.The result of questionnaire also reflects these problems, mostly
The experimenter that number does the primary granulosa cell culture of ovary has this puzzlement --- and experiment is required for expending great effort pin every time
Many mouse ovarians are pierced, enough primary granular cell quantity could be obtained, this enthusiasm for greatly reducing researcher also hinders
The progress of correlative study is hindered.
The content of the invention
Wasted time and energy the low present situation of efficiency to solve the existing primary granular cell extracting method of ovary, the invention provides
A kind of electronic ovarian follicle revulseur.
In order to realize the purpose of the present invention, the technical solution adopted by the present invention is:
A kind of electronic ovarian follicle revulseur, including fuselage support, the inside of the fuselage support are provided with puncture channel, and described puncture is led to
Matched guide rod is provided with road, one end of the guide rod is past via puncture channel described in electric drive unit drives edge
Multiple motion, the other end stretch out the puncture channel and are connected with asepsis injector syringe needle.
The electric drive unit includes motor and the toggle being driven by motor, the crank connecting link machine
Structure includes driving gear, the reduction gearing engaged with driving gear, and the driving gear forms train of reduction gears with reduction gearing,
The power transmission shaft of bias is provided with the card of the reduction gearing, the transmission outer shaft sleeve is equipped with axle sleeve, and the axle sleeve passes through company
Bar is connected with guide rod, the wheel axis connection of the motor and driving gear, and battery, the electricity are additionally provided with beside the motor
Machine switch is arranged on fuselage support side.
Battery is provided with beside the motor.
The asepsis injector syringe needle and guide rod connected mode are clamping or grafting.
The asepsis injector syringe needle is dismountable disposable sterilized injector syringe needle.
The beneficial effects of the present invention are:
Whole device substantially reduces by the way that simply cleverly design improves the deficiency that existing ovarian follicle needle point method obtains granular cell
The work load of experimenter, has been obviously improved conventional efficient.The mock-up produced also achieves well in an experiment
Effect, compared to former manual needle point method, the more better quality of granular cell obtained using electronic ovarian follicle revulseur,
Required time is shorter, efficient quick, obtains the consistent favorable comment of multidigit user.
Brief description of the drawings
The present invention is described further with case study on implementation below in conjunction with the accompanying drawings.
Fig. 1 is the top view of the present invention;
Fig. 2 is the front view of the present invention.
Wherein, 1 asepsis injector syringe needle, 2 guide rods, 3 switches, 4 puncture channels, 5. fuselage supports, 6. connecting rods, 601 axles
Set, 7 reduction gearing, 701 power transmission shafts, 8 batteries, 9 motors, 901 driving gears.
Embodiment
The present invention is further described with reference to the accompanying drawings and examples:
Referring to Fig. 1-2.
The invention discloses a kind of electronic ovarian follicle revulseur, including fuselage support 5, the inside of the fuselage support 5 is provided with
Puncture channel 4, the puncture channel 4 is interior to be provided with matched guide rod 2, and one end of the guide rod 2 is via motorized motions
Puncture channel 4 moves back and forth described in unit drives edge, and the other end stretches out the puncture channel 4 and connected with asepsis injector syringe needle 1
Connect.
The electric drive unit includes motor 9 and the toggle being driven by motor 9, the crank connecting link
Mechanism includes driving gear 901, the reduction gearing 7 engaged with driving gear 901, the driving gear 901 and the structure of reduction gearing 7
Into train of reduction gears, the power transmission shaft 701 of bias is provided with the card of the reduction gearing 7, is set with outside the power transmission shaft 701
Axle sleeve 601, the axle sleeve 601 are connected by connecting rod 6 with guide rod 2, the wheel axis connection of the motor 9 and driving gear 901, institute
State the side of motor 9 and be additionally provided with battery 8, the motor switch 3 is arranged on the side of fuselage support 5.
The side of motor 9 is provided with battery 8.
The asepsis injector syringe needle 1 and the connected mode of guide rod 2 are clamping or grafting.
The asepsis injector syringe needle 1 is dismountable disposable sterilized injector syringe needle.
The use principle of the present invention is summarized as follows:
Battery 8 connects motor 9 and closed by switching 3 control circuits.Asepsis injector syringe needle 1 is inserted in first during use and led
To on bar 2, hand holds fuselage support 5 in lip pencil is held, and after forefinger presses lower switch 3, motor 9, which is powered to starting to rotate, drives corresponding slow down
Gear 7 rotates, and guide rod 2 moves back and forth along the puncture channel 4.Under the fixation of puncture channel 4, the rolling of guide rod 2
Dynamic amplitude substantially reduces so that the acupuncture site when syringe needle 1 that the termination of guide rod 2 is fixed moves up and down becomes very smart
It is accurate.Under Stereo microscope, fixed mouse ovarian is directed at, carries out electronic acupuncture, so as to obtain primary granular cell.
Embodiments of the invention are the foregoing is only, are not intended to limit the scope of the invention, it is every to utilize this hair
The equivalents or be directly or indirectly used in related technical field that bright specification and accompanying drawing content are made, are similarly included in
In the scope of patent protection of the present invention.
Claims (5)
1. a kind of electronic ovarian follicle revulseur, including fuselage support(5), it is characterised in that:The fuselage support(5)Inside be provided with
Puncture channel(4), the puncture channel(4)It is interior to be provided with matched guide rod(2), the guide rod(2)One end via
Puncture channel described in electric drive unit drives edge(4)Move back and forth, the other end stretches out the puncture channel(4)And with sterile note
Emitter syringe needle(1)Connection.
2. electronic ovarian follicle revulseur according to claim 1, it is characterised in that:The electric drive unit includes motor
(9)And pass through motor(9)The toggle of transmission, the toggle include driving gear(901), with active
Gear(901)The reduction gearing of engagement(7), the driving gear(901)With reduction gearing(7)Train of reduction gears is formed, it is described
Reduction gearing(7)Card on be provided with bias power transmission shaft(701), the power transmission shaft(701)It is set with axle sleeve outside(601),
The axle sleeve(601)Pass through connecting rod(6)With guide rod(2)Connection, the motor(9)With driving gear(901)Wheel axis connection,
The motor(9)Side is additionally provided with battery(8), the motor switch(3)It is arranged on fuselage support(5)Side.
A kind of 3. electronic ovarian follicle revulseur according to claim 2, it is characterised in that:The motor(9)Side is provided with
Battery(8).
A kind of 4. electronic ovarian follicle revulseur according to claim 2, it is characterised in that:The asepsis injector syringe needle(1)
With guide rod(2)Connected mode is clamping or grafting.
A kind of 5. electronic ovarian follicle revulseur according to claim 1, it is characterised in that:The asepsis injector syringe needle(1)
For dismountable disposable sterilized injector syringe needle.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN201711235468.9A CN107794213A (en) | 2017-11-30 | 2017-11-30 | A kind of electronic ovarian follicle revulseur |
Applications Claiming Priority (1)
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CN201711235468.9A CN107794213A (en) | 2017-11-30 | 2017-11-30 | A kind of electronic ovarian follicle revulseur |
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Publication Number | Publication Date |
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CN107794213A true CN107794213A (en) | 2018-03-13 |
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CN201711235468.9A Pending CN107794213A (en) | 2017-11-30 | 2017-11-30 | A kind of electronic ovarian follicle revulseur |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109182104A (en) * | 2018-11-08 | 2019-01-11 | 上海交通大学医学院附属瑞金医院 | Full-automatic ovarian follicle needing machine |
Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
RU2071294C1 (en) * | 1995-01-19 | 1997-01-10 | Маленко Галина Петровна | Device for isolating oocytes from ovaries of mammals |
WO2000071051A1 (en) * | 1999-05-24 | 2000-11-30 | Genetics Australia Co-Operative Limited | Method and apparatus for extracting oocytes from ovaries |
EP1967147A2 (en) * | 2007-03-09 | 2008-09-10 | Hans-Peter Steiner | Puncture device for retrieving an organic sample |
CN101347346A (en) * | 2008-06-26 | 2009-01-21 | 张震 | Pressure regulation instrument of fine needle puncture |
US20120116245A1 (en) * | 2009-06-25 | 2012-05-10 | Hans-Peter Steiner | Puncture device |
CN204318970U (en) * | 2014-08-29 | 2015-05-13 | 深圳华大基因研究院 | Get egg apparatus |
CN106691624A (en) * | 2017-01-20 | 2017-05-24 | 河南省农业科学院畜牧兽医研究所 | Handheld electric ovum sampling device |
CN208308829U (en) * | 2017-11-30 | 2019-01-01 | 南昌大学 | A kind of electronic ovarian follicle revulseur |
-
2017
- 2017-11-30 CN CN201711235468.9A patent/CN107794213A/en active Pending
Patent Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
RU2071294C1 (en) * | 1995-01-19 | 1997-01-10 | Маленко Галина Петровна | Device for isolating oocytes from ovaries of mammals |
WO2000071051A1 (en) * | 1999-05-24 | 2000-11-30 | Genetics Australia Co-Operative Limited | Method and apparatus for extracting oocytes from ovaries |
EP1967147A2 (en) * | 2007-03-09 | 2008-09-10 | Hans-Peter Steiner | Puncture device for retrieving an organic sample |
CN101347346A (en) * | 2008-06-26 | 2009-01-21 | 张震 | Pressure regulation instrument of fine needle puncture |
US20120116245A1 (en) * | 2009-06-25 | 2012-05-10 | Hans-Peter Steiner | Puncture device |
CN204318970U (en) * | 2014-08-29 | 2015-05-13 | 深圳华大基因研究院 | Get egg apparatus |
CN106691624A (en) * | 2017-01-20 | 2017-05-24 | 河南省农业科学院畜牧兽医研究所 | Handheld electric ovum sampling device |
CN208308829U (en) * | 2017-11-30 | 2019-01-01 | 南昌大学 | A kind of electronic ovarian follicle revulseur |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109182104A (en) * | 2018-11-08 | 2019-01-11 | 上海交通大学医学院附属瑞金医院 | Full-automatic ovarian follicle needing machine |
CN109182104B (en) * | 2018-11-08 | 2023-11-03 | 上海交通大学医学院附属瑞金医院 | Full-automatic follicular needling machine |
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