CN208308829U - A kind of electronic ovarian follicle revulseur - Google Patents
A kind of electronic ovarian follicle revulseur Download PDFInfo
- Publication number
- CN208308829U CN208308829U CN201721636246.3U CN201721636246U CN208308829U CN 208308829 U CN208308829 U CN 208308829U CN 201721636246 U CN201721636246 U CN 201721636246U CN 208308829 U CN208308829 U CN 208308829U
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- ovarian follicle
- electronic
- guide rod
- puncture channel
- revulseur
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Abstract
The utility model relates to technical field of cell biology more particularly to a kind of electronic ovarian follicle revulseurs.The electronic ovarian follicle revulseur of kind, including fuselage support, the inside of the fuselage support is equipped with puncture channel, matched guide rod is equipped in the puncture channel, one end of the guide rod is moved back and forth via puncture channel described in electric drive unit drives edge, and the other end stretches out the puncture channel and connect with asepsis injector syringe needle.Whole device improves the deficiency that existing ovarian follicle needle point method obtains granular cell by simple cleverly design, greatly reduces the work load of experimenter, has been obviously improved conventional efficient.
Description
Technical field
The utility model relates to technical field of cell biology more particularly to a kind of electronic ovarian follicle revulseurs.
Background technique
Ovary is the Main Reproductive Organs of female mammal, and ovarian follicle is the main constituents in ovary.Primordial follicle
It is made of the single layer scales granular cell of egg mother cell and surrounding, granular cell participates in regulation ovarian follicle from growth and development to maturation
The whole process of ovulation, they will successively undergo primary, secondary follicle and antral follicles stage, ovulate subsequently into graaffian follicle
Phase.From primordial follicle to Graafian follicle or atretic follicle is a complicated and accurate process, the granular cell of differentiation with
Egg mother cell, which is formed, closely combines and provides the exchange of information substance, while granular cell can secrete progesterone, estrogen etc.
Hormone and expression specificity receptor (FSHR) are for example ovulated with this to coordinate the mature and subsequent activity of Oocyte Development,
Promote fertilization and early embryonic development etc..Granular cell and egg mother cell form entirety functionally, when granular cell withers
Die the locking that will cause ovarian follicle, ovarian follicle once locking can not normal development, eventually lead to female reproduction function associated disorders
With disease.
Primary gonad granulocyte in-vitro culture model is established, convenient for carrying out granular cell growth under in vitro conditions, is increased
It grows, the research of differentiation and apoptosis, is provided to study the researchs such as female Ovarian Physiological Function, ovary related disease and Reproductive toxicology
Good means.The gene of mouse is similar to mankind's height, and all kinds of biochemical reactions and people are almost the same, and have and be convenient for
The low equal many merits of feeding cost, therefore be the optimal selection of experimental animal.Firstly, the ovarian granulosa for obtaining a large amount of high quality is thin
Born of the same parents are the important foundations of this aspect research.Mouse granulosa cells separation is mostly shown using experimenter stereoscopic in document at present
Ovarian follicle is punctured with stainless steel syringe needle under micro mirror, granular cell is made to be discharged into the method in culture medium [1-4].This method is turned out
The primary granular cell purity is high come, growth and proliferation of cell ability are strong.But there is also shortcoming, experimenter first is hand-held
Stainless pin scalp acupuncture pierces ovarian follicle, and efficiency very lowly takes time and effort, and needle difficult to realize pierces each ovarian follicle and makes granular cell
Thoroughly release, this will largely reduce the collecting amount of granular cell.And if experimenter is in order to collect more granular cells
And sufficiently needle pierces ovarian follicle, can usually take long time again, granular cell as being transferred in the environment of suitable growth not in time, the death rate
It can increase.Meanwhile the operating time is too long, cell is easier contaminated.The result of questionnaire also reflects these problems, mostly
The experimenter that number does the primary granulosa cell culture of ovary has this puzzlement --- and experiment requires to expend great effort needle every time
Many mouse ovarians are pierced, enough primary granular cell quantity could be obtained, this enthusiasm for greatly reducing researcher also hinders
The progress of correlative study is hindered.
Utility model content
For the status for solving the existing primary time-consuming and laborious inefficiency of granular cell extracting method of ovary, the utility model is mentioned
A kind of electronic ovarian follicle revulseur is supplied.
In order to realize the purpose of this utility model, the technical solution adopted in the utility model are as follows:
A kind of electronic ovarian follicle revulseur, including fuselage support, the inside of the fuselage support is equipped with puncture channel, described to wear
Matched guide rod is equipped in transfixation road, one end of the guide rod is logical via puncture described in electric drive unit drives edge
Road moves back and forth, and the other end stretches out the puncture channel and connect with asepsis injector syringe needle.
The electric drive unit includes motor and the crank link mechanism by motor drive, the crank connecting link machine
Structure includes driving gear, the reduction gearing engaged with driving gear, and the driving gear and reduction gearing constitute train of reduction gears,
Eccentric transmission shaft is provided in the disk of the reduction gearing, the transmission outer shaft sleeve is equipped with axle sleeve, and the axle sleeve passes through company
Bar is connect with guide rod, the wheel axis connection of the motor and driving gear, is additionally provided with battery, the electricity beside the motor
Machine switch is arranged in fuselage support side.
The asepsis injector syringe needle and guide rod connection type are clamping or grafting.
The asepsis injector syringe needle is dismountable disposable sterilized injector syringe needle.
The utility model has the beneficial effects that:
Whole device improves the deficiency that existing ovarian follicle needle point method obtains granular cell by simple cleverly design, significantly
The work load for reducing experimenter, has been obviously improved conventional efficient.The mock-up produced also achieves very in an experiment
Good effect, compared to pervious manual needle point method, the more quality of granular cell obtained using electronic ovarian follicle revulseur
More preferably, required time is shorter, efficient quick, obtains the consistent favorable comment of multidigit user.
Detailed description of the invention
The utility model is described further with case study on implementation with reference to the accompanying drawing.
Fig. 1 is the top view of the utility model；
Fig. 2 is the front view of the utility model.
Wherein, 1 asepsis injector syringe needle, 2 guide rods, 3 switches, 4 puncture channels, 5. fuselage supports, 6. connecting rods, 601 axis
Set, 7 reduction gearing, 701 transmission shafts, 8 batteries, 9 motors, 901 driving gears.
Specific embodiment
The present invention will be further described with reference to the accompanying drawings and examples:
Referring to Fig. 1-2.
The utility model discloses a kind of electronic ovarian follicle revulseur, including fuselage support 5, the inside of the fuselage support 5
Equipped with puncture channel 4, it is equipped with matched guide rod 2 in the puncture channel 4, one end of the guide rod 2 is via electronic
Puncture channel 4 described in driving unit drives edge moves back and forth, the other end stretch out the puncture channel 4 and with asepsis injector syringe needle
1 connection.
The electric drive unit includes motor 9 and the crank link mechanism that is driven by motor 9, the crank connecting link
Mechanism includes driving gear 901, the reduction gearing 7 engaged with driving gear 901, the driving gear 901 and 7 structure of reduction gearing
At train of reduction gears, it is provided with eccentric transmission shaft 701 in the disk of the reduction gearing 7, is set with outside the transmission shaft 701
Axle sleeve 601, the axle sleeve 601 are connect by connecting rod 6 with guide rod 2, the wheel axis connection of the motor 9 and driving gear 901, institute
It states and is additionally provided with battery 8 beside motor 9, the motor switch 3 is arranged in 5 side of fuselage support.
The asepsis injector syringe needle 1 is clamping or grafting with 2 connection type of guide rod.
The asepsis injector syringe needle 1 is dismountable disposable sterilized injector syringe needle.
The use principle of the utility model is summarized as follows:
Battery 8 connects motor 9 and is closed by 3 control circuit of switch.Asepsis injector syringe needle 1 is inserted first when use
On guide rod 2, hand holds fuselage support 5 in lip pencil is held, and after index finger presses lower switch 3, it is corresponding that the energization of motor 9 starts turning drive
Reduction gearing 7 rotates, and guide rod 2 is moved back and forth along the puncture channel 4.Under the fixed function of puncture channel 4, guide rod 2
Shaking amplitude substantially reduce so that needle thorn site when the fixed syringe needle 1 in 2 end of guide rod moves up and down becomes ten
Divide accurate.Under Stereo microscope, the mouse ovarian of alignment and fixation carries out electronic needle thorn, to obtain primary granular cell.
The above description is only the embodiments of the present invention, and therefore it does not limit the scope of the patent of the utility model, all
Equivalents made based on the specification and figures of the utility model are applied directly or indirectly in relevant technical field,
It is also included in the patent protection scope of the utility model.
Claims (4)
1. a kind of electronic ovarian follicle revulseur, including fuselage support (5), it is characterised in that: the inside of the fuselage support (5) is equipped with
Puncture channel (4), is equipped with matched guide rod (2) in the puncture channel (4), one end of the guide rod (2) via
Puncture channel described in electric drive unit drives edge (4) moves back and forth, the other end stretch out the puncture channel (4) and with sterile note
Emitter syringe needle (1) connection.
2. electronic ovarian follicle revulseur according to claim 1, it is characterised in that: the electric drive unit includes motor
(9) and by motor (9) crank link mechanism being driven, the crank link mechanism includes driving gear (901), with active
The reduction gearing (7) of gear (901) engagement, the driving gear (901) and reduction gearing (7) constitute train of reduction gears, described
It is provided with eccentric transmission shaft (701) in the disk of reduction gearing (7), is set with axle sleeve (601) outside the transmission shaft (701),
The axle sleeve (601) is connect by connecting rod (6) with guide rod (2), the wheel axis connection of the motor (9) and driving gear (901),
It is additionally provided with battery (8) beside the motor (9), motor switch (3) is arranged in fuselage support (5) side.
3. a kind of electronic ovarian follicle revulseur according to claim 2, it is characterised in that: the asepsis injector syringe needle (1)
It is clamping or grafting with guide rod (2) connection type.
4. a kind of electronic ovarian follicle revulseur according to claim 1, it is characterised in that: the asepsis injector syringe needle (1)
For dismountable disposable sterilized injector syringe needle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721636246.3U CN208308829U (en) | 2017-11-30 | 2017-11-30 | A kind of electronic ovarian follicle revulseur |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721636246.3U CN208308829U (en) | 2017-11-30 | 2017-11-30 | A kind of electronic ovarian follicle revulseur |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208308829U true CN208308829U (en) | 2019-01-01 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721636246.3U Expired - Fee Related CN208308829U (en) | 2017-11-30 | 2017-11-30 | A kind of electronic ovarian follicle revulseur |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208308829U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107794213A (en) * | 2017-11-30 | 2018-03-13 | 南昌大学 | A kind of electronic ovarian follicle revulseur |
-
2017
- 2017-11-30 CN CN201721636246.3U patent/CN208308829U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107794213A (en) * | 2017-11-30 | 2018-03-13 | 南昌大学 | A kind of electronic ovarian follicle revulseur |
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190101 Termination date: 20191130 |