CN204228674U - The liquid chromatographic system of tobacco flavor material is prepared in a kind of separation - Google Patents
The liquid chromatographic system of tobacco flavor material is prepared in a kind of separation Download PDFInfo
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- CN204228674U CN204228674U CN201420659282.1U CN201420659282U CN204228674U CN 204228674 U CN204228674 U CN 204228674U CN 201420659282 U CN201420659282 U CN 201420659282U CN 204228674 U CN204228674 U CN 204228674U
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- backwash valve
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Abstract
The utility model provides a kind of separation to prepare the liquid chromatographic system of tobacco flavor material, the fraction collection instrument that described liquid chromatographic system is comprised mobile phase solvent bottle, sampling valve, pre-column, chromatographic column, UV-detector and is connected by pipeline with described UV-detector output terminal, described sampling valve is a six-way valve, and the outlet of described pre-column is connected with described chromatographic column; Also comprise backwash valve, described backwash valve is a four-way valve, the a position of described backwash valve is connected by pipeline with the b position of described sampling valve, the b position of described backwash valve is connected by pipeline with the entrance of described pre-column, the c position of described backwash valve is connected with described UV-detector input end, and the d position of described backwash valve is connected by pipeline with described column outlet.Utilize described backwash valve can pre-column described in on-line cleaning and chromatographic column, improve reliability, as long as and switch described backwash valve just can clean described in pre-column and chromatographic column, simple to operate.
Description
Technical field
The utility model relates to a kind of liquid chromatography technology, particularly relates to the liquid chromatographic system that tobacco flavor material is prepared in a kind of separation.
Background technology
One of Main Means that preparative liquid chromatography is tobacco, prepared by smoke perfume separating substances.During preparation, the chromatographic column generation pressure durations change on usual liquid chromatograph is large or column system is contaminated, can require that chromatographic column is pulled down anti-dress by user, uses low flow velocity and enters specific sample and clean.Therefore, need when safeguarding chromatographic column to carry out dismounting, complex operation to pillar, easily cause organic solvent to reveal and make column system introduce air, thus affecting post effect, reliability is low.
In addition, often dead volume is larger for preparation liquid phase instrument, and particularly, between UV-detector output terminal and fraction collection instrument, the dead volume of pipeline is greater than 1mL, because dead volume is larger, therefore is not suitable for the few preparation of sample size; Meanwhile, sample can merge due to diffusion after releasing once more, and is all deposited in pipeline, so just causes fraction collection to there is very large problem.
Utility model content
The shortcoming of prior art in view of the above, the purpose of this utility model is to provide a kind of separation to prepare the liquid chromatographic system of tobacco flavor material, and it is simple to operate, and on-line cleaning pre-column and chromatographic column can be realized, reliability is high.
For achieving the above object and other relevant objects, the utility model provides a kind of separation to prepare the liquid chromatographic system of tobacco flavor material, described liquid chromatographic system comprises mobile phase solvent bottle, sampling valve, pre-column, chromatographic column, UV-detector and the fraction collection instrument be connected by pipeline with described UV-detector output terminal, described sampling valve is a six-way valve, the f position of described sampling valve is injection port, the a position of described sampling valve is connected with the two ends of quantitative loop respectively with d position, the c position of described sampling valve is connected with described mobile phase solvent bottle by pipeline, pipeline between described sampling valve and described mobile phase solvent bottle is provided with pump, the e position of described sampling valve is connected with waste liquid bottle by pipeline, the outlet of described pre-column is connected with described chromatographic column, it is characterized in that, also comprise backwash valve, described backwash valve is a four-way valve, the a position of described backwash valve is connected by pipeline with the b position of described sampling valve, the b position of described backwash valve is connected by pipeline with the entrance of described pre-column, and the c position of described backwash valve is connected with described UV-detector input end, and the d position of described backwash valve is connected by pipeline with described column outlet.
Further, described pipeline external diameter is 0.15875cm, and internal diameter is 0.01016-0.0762cm.
Further, the internal diameter connecting the pipeline of described UV-detector output terminal and described fraction collection instrument is 0.01524cm, and the length of this section of pipeline is 20-50cm.
Further, described pipeline is made by corrosion-resistant material material.
Further, corrosion-resistant material material is teflon, fluorinated ethylene propylene, or stainless steel.
Further, described chromatographic column is silicagel column.
As mentioned above, the liquid chromatographic system of tobacco flavor material is prepared in a kind of separation of the present utility model, has following beneficial effect:
Because described liquid chromatographic system also comprises backwash valve, described backwash valve is a four-way valve, the a position of described backwash valve is connected by pipeline with the b position of described sampling valve, the b position of described backwash valve is connected by pipeline with the entrance of described pre-column, the c position of described backwash valve is connected with described UV-detector input end, and the d position of described backwash valve is connected by pipeline with described column outlet; Utilize described backwash valve can pre-column described in on-line cleaning and chromatographic column, improve reliability, as long as and switch described backwash valve just can clean described in pre-column and chromatographic column, simple to operate.
Accompanying drawing explanation
Fig. 1 is shown as the schematic diagram that the liquid chromatographic system of tobacco flavor material is prepared in a kind of separation of the present utility model.
Element numbers explanation
1 mobile phase solvent bottle
2 sampling valves
21 Measuring glasses
3 pre-columns
4 chromatographic columns
5 UV-detector
6 fraction collection instrument
7 pumps
8 waste liquid bottles
9 backwash valve
Embodiment
By particular specific embodiment, embodiment of the present utility model is described below, person skilled in the art scholar the content disclosed by this instructions can understand other advantages of the present utility model and effect easily.
Refer to Fig. 1.Notice, structure, ratio, size etc. that this instructions institute accompanying drawings illustrates, content all only in order to coordinate instructions to disclose, understand for person skilled in the art scholar and read, and be not used to limit the enforceable qualifications of the utility model, therefore the not technical essential meaning of tool, the adjustment of the modification of any structure, the change of proportionate relationship or size, do not affecting under effect that the utility model can produce and the object that can reach, all should still drop in scope that technology contents that the utility model discloses can contain.Simultaneously, quote in this instructions as " on ", D score, "left", "right", " centre " and " one " etc. term, also only for ease of understanding of describing, and be not used to limit the enforceable scope of the utility model, the change of its relativeness or adjustment, under changing technology contents without essence, when being also considered as the enforceable category of the utility model.
As shown in Figure 1, the utility model provides a kind of separation to prepare the liquid chromatographic system of tobacco flavor material, described liquid chromatographic system comprises mobile phase solvent bottle 1, sampling valve 2, pre-column 3, chromatographic column 4, UV-detector 5 and the fraction collection instrument 6 be connected by pipeline with described UV-detector 5 output terminal, described sampling valve 2 is a six-way valve, the f position of described sampling valve 2 is injection port, the a position of described sampling valve 2 is connected with the two ends of quantitative loop 21 respectively with d position, the c position of described sampling valve 2 is connected with described mobile phase solvent bottle 1 by pipeline, pipeline between described sampling valve 2 and described mobile phase solvent bottle 1 is provided with pump 7, the e position of described sampling valve 2 is connected with waste liquid bottle 8 by pipeline, the outlet of described pre-column 3 is connected with described chromatographic column 4, liquid chromatographic system also comprises backwash valve 9, described backwash valve 9 is a four-way valve, the a position of described backwash valve 9 is connected by pipeline with the b position of described sampling valve 2, the b position of described backwash valve 9 is connected by pipeline with the entrance of described pre-column 3, the c position of described backwash valve 9 is connected with described UV-detector 5 input end, and the d position of described backwash valve 9 is exported with described chromatographic column 4 and is connected by pipeline, preferably, described chromatographic column 4 is silicagel column.Utilize described backwash valve 9 can pre-column 3 described in on-line cleaning and chromatographic column 4, improve reliability, as long as and switch described backwash valve 9 just can clean described in pre-column 3 and chromatographic column 4, simple to operate.Meanwhile, utilize this online backflushing method effectively can save alcohol solvent, contrast can obtain with traditional elution method, only need 1/3 volume of original solvent described chromatographic column 4 can be rinsed well, effectively saved the solvent of 2/3, economize solvent as seen and save time.
Often dead volume is larger for traditional preparation liquid phase, and particularly, dead volume is greater than 1mL, because dead volume is larger, therefore is not suitable for the few preparation of sample size; Meanwhile, sample can merge due to diffusion after releasing once more, and is all deposited in pipeline, so just causes fraction collection to there is very large problem.Improve further as this utility model, in order to reduce dead volume, described pipeline external diameter is 0.15875cm, and internal diameter is 0.01016-0.0762cm.Preferably, described pipeline is made by corrosion-resistant material material.Corrosion-resistant material material is teflon, fluorinated ethylene propylene, or stainless steel.Preferably, the internal diameter connecting the pipeline of described UV-detector 5 output terminal and described fraction collection instrument 6 is 0.01524cm, and the length of this section of pipeline is 20-50cm.Be only 8.8 μ L from detecting device to the stream dead volume of fraction collection instrument 6, effectively prevent the problems referred to above, obtain preferably cut, be applicable to very much a small amount of sample feeding and collect, see Fig. 1.
In sum, the liquid chromatographic system that tobacco flavor material is prepared in a kind of separation of the present utility model is not only simple to operate, and on-line cleaning pre-column and chromatographic column can be realized, reliability is high.So the utility model effectively overcomes various shortcoming of the prior art and tool high industrial utilization.
Above-described embodiment is illustrative principle of the present utility model and effect thereof only, but not for limiting the utility model.Any person skilled in the art scholar all without prejudice under spirit of the present utility model and category, can modify above-described embodiment or changes.Therefore, have in all art and usually know that the knowledgeable modifies or changes not departing from all equivalences completed under the spirit and technological thought that the utility model discloses, must be contained by claim of the present utility model.
Claims (6)
1. the liquid chromatographic system of tobacco flavor material is prepared in a separation, described liquid chromatographic system comprises mobile phase solvent bottle (1), sampling valve (2), pre-column (3), chromatographic column (4), UV-detector (5) and the fraction collection instrument (6) be connected by pipeline with described UV-detector (5) output terminal, described sampling valve (2) is a six-way valve, the f position of described sampling valve (2) is injection port, the a position of described sampling valve (2) is connected with the two ends of quantitative loop respectively with d position, the c position of described sampling valve (2) is connected with described mobile phase solvent bottle (1) by pipeline, pipeline between described sampling valve (2) and described mobile phase solvent bottle (1) is provided with pump (7), the e position of described sampling valve (2) is connected with waste liquid bottle (8) by pipeline, the outlet of described pre-column (3) is connected with described chromatographic column (4), it is characterized in that, also comprise backwash valve (9), described backwash valve (9) is a four-way valve, the a position of described backwash valve (9) is connected by pipeline with the b position of described sampling valve (2), the b position of described backwash valve (9) is connected by pipeline with the entrance of described pre-column (3), the c position of described backwash valve (9) is connected with described UV-detector (5) input end, and the d position of described backwash valve (9) is exported with described chromatographic column (4) and is connected by pipeline.
2. the liquid chromatographic system of tobacco flavor material is prepared in a kind of separation according to claim 1, it is characterized in that: described pipeline external diameter is 0.15875cm, and internal diameter is 0.01016-0.0762cm.
3. the liquid chromatographic system of tobacco flavor material is prepared in a kind of separation according to claim 2, it is characterized in that: the internal diameter connecting the pipeline of described UV-detector (5) output terminal and described fraction collection instrument (6) is 0.01524cm, and the length of this section of pipeline is 20-50cm.
4. the liquid chromatographic system of tobacco flavor material is prepared in a kind of separation according to claim 1, it is characterized in that: described pipeline is made by corrosion-resistant material material.
5. the liquid chromatographic system of tobacco flavor material is prepared in a kind of separation according to claim 4, it is characterized in that: corrosion-resistant material material is teflon, fluorinated ethylene propylene, or stainless steel.
6. the liquid chromatographic system of tobacco flavor material is prepared in a kind of separation according to claim 1, it is characterized in that: described chromatographic column (4) is silicagel column.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420659282.1U CN204228674U (en) | 2014-11-05 | 2014-11-05 | The liquid chromatographic system of tobacco flavor material is prepared in a kind of separation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420659282.1U CN204228674U (en) | 2014-11-05 | 2014-11-05 | The liquid chromatographic system of tobacco flavor material is prepared in a kind of separation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204228674U true CN204228674U (en) | 2015-03-25 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420659282.1U Active CN204228674U (en) | 2014-11-05 | 2014-11-05 | The liquid chromatographic system of tobacco flavor material is prepared in a kind of separation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN204228674U (en) |
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2014
- 2014-11-05 CN CN201420659282.1U patent/CN204228674U/en active Active
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