CN204138668U - A kind of ultrasonic wave microorganism diverting device - Google Patents

A kind of ultrasonic wave microorganism diverting device Download PDF

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Publication number
CN204138668U
CN204138668U CN201420560535.XU CN201420560535U CN204138668U CN 204138668 U CN204138668 U CN 204138668U CN 201420560535 U CN201420560535 U CN 201420560535U CN 204138668 U CN204138668 U CN 204138668U
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China
Prior art keywords
diverting device
container
ultrasonic transducer
microorganism
dispersion
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Active
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CN201420560535.XU
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Chinese (zh)
Inventor
朱国峰
莫白自
张红波
侯剑
赵维国
毕利军
邓教宇
陶生策
杭海英
黎嘉维
杨晓
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Guangdong Skywork Biotechnology Co Ltd
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Tb Healthcare Co ltd
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Priority to CN201420560535.XU priority Critical patent/CN204138668U/en
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Abstract

The utility model discloses a kind of ultrasonic wave microorganism diverting device, the ultrasonic transducer that described diverting device comprises ultrasonic generator and is connected electrically, also comprise one for hold microorganism to be disperseed, container, container outer wall and the ultrasonic transducer end face close contact that can move freely.Diverting device of the present utility model, directly acts on the outer wall holding and wait the container disperseing microorganism, is directly delivered to and treats without the need to liquid medium in dispersed sample after ultrasonic transducer by ultrasonic wave during dispersion, thus realize dispersion.In dispersion process, container can lidstock, ensure that biological safety does not affect wall of container transparency simultaneously, avoids the impact on follow-up test.The utility model can be used in microorganism dispersion experiment.

Description

A kind of ultrasonic wave microorganism diverting device
Technical field
The utility model relates to a kind of ultrasonic wave microorganism diverting device.
Background technology
In the process of Bioexperiment, in microbial solution, the normally agglomerating existence of microbe, as mycobacterium tuberculosis etc., therefore need the dispersion of grinding bacterial mass before bacterium solution preparation in the solution.
Existing conventional disperse method is mechanical milling method, and as mill bacterium bottle method, mill tube method or liquid-transfering gun pressure-vaccum mode of averaging etc., these manipulation requires are manual to carry out, and there is dispersion problem irregular, consuming time.Further, above operation is all carried out in open container, and has the microorganisms such as the bacterium of infectivity and toxicity in the process of grinding and dilute, and there is the chance contacted with skin and respiratory system, has the hidden danger of latent infection.
In Bioexperiment, normally to carry out analytical test to the microbial solution after dispersion, some tests have strict demand to the transparency of vessel, as turbidity test etc., sample after traditional dispersing method process, the many extensions of vessel wall, with liquid, need again to process it, to avoid surface liquid to remain interference to test result, this makes troubles to operation.Therefore also need badly a kind of easily, on wall of container transparency without the diverting device affected.
Ultrasonic technology is widely used at biochemistry, carry out cleaning or microbial cell breakage etc., but the technology utilizing ultrasonic wave to carry out microorganism dispersion also rarely has introduction as utilized it.
Summary of the invention
For solving the problem, the utility model provides a kind of ultrasonic wave microorganism diverting device, and dispersion efficiency is high, and biological safety is good, and easy to operation.
The solution that the utility model solves its technical problem is: provide a kind of ultrasonic wave microorganism diverting device, described diverting device comprises ultrasonic generator and ultrasonic transducer, is electrically connected between described ultrasonic generator and ultrasonic transducer.Described diverting device also comprises a container, for holding microorganism to be disperseed, and the outer wall of described container and the end face close contact of described ultrasonic transducer; Described container can move freely, to be convenient for changing, and according to the size etc. of area of dispersion effect adjustment close contact.
Such ultrasonic wave directly acts on the outer wall holding and wait the container disperseing microorganism after ultrasonic transducer, ultrasonic wave is directly delivered to by wall to be treated in dispersed sample, do not need to pass through liquid medium, then ultrasonic cavitation is utilized, reach and microbic mass is carried out disperseing to conciliate the effect of reuniting, thus reach the object of dispersion.Both can seal dispersion in dispersion process, it also avoid wall surface simultaneously and hang attached liquid body, affect subsequent operations.Described container outer wall had both comprised outer side wall and had also comprised outer bottom.
Further, for reaching better dispersion effect, the bottom of described container and the end face close contact of described ultrasonic transducer.Further, the end face of described ultrasonic transducer has a groove, coordinate with the bottom of described container, make described container bottom have at least a border and ultrasonic transducer end face to have close contact.
Described diverting device also comprises a stationary installation, for fixing described container, to avoid container displacement in dispersion process, ensures the close contact of wall of container and ultrasonic transducer end face, while also handled easily.
The frequency of the ultrasonic transducer of described diverting device is 20KHz ~ 80KHz, and power is 5W ~ 200W.With this understanding, dispersion effect is better, and on microbic activity without impact.
The beneficial effects of the utility model are: during ultrasonic wave microorganism diverting device dispersion of the present utility model, ultrasonic wave is directly acted on after ultrasonic transducer the outer wall holding and wait the container disperseing microorganism, be directly delivered to and treat without the need to liquid medium in dispersed sample, thus realize dispersion.In dispersion process, container can lidstock, ensure that biological safety does not affect wall of container transparency simultaneously, avoids the impact on follow-up test.The utility model can be used in microorganism dispersion experiment.
Accompanying drawing explanation
In order to be illustrated more clearly in the technical scheme in the utility model embodiment, below the accompanying drawing used required in describing embodiment is briefly described.Obviously, described accompanying drawing is a part of embodiment of the present utility model, instead of whole embodiment, and those skilled in the art, under the prerequisite not paying creative work, can also obtain other designs and accompanying drawing according to these accompanying drawings.
Fig. 1 is diverting device structural representation of the present utility model;
Fig. 2 is the dispersion effect schematic diagram of diverting device of the present utility model;
Fig. 3 is the dispersion effect schematic diagram of prior art.
Embodiment
Be clearly and completely described below with reference to embodiment and the accompanying drawing technique effect to design of the present utility model, concrete structure and generation, to understand the purpose of this utility model, characteristic sum effect fully.Obviously; described embodiment is a part of embodiment of the present utility model, instead of whole embodiment, based on embodiment of the present utility model; other embodiments that those skilled in the art obtains under the prerequisite not paying creative work, all belong to the scope of the utility model protection.Each technical characteristic in the invention, can combination of interactions under the prerequisite of not conflicting conflict.
The utility model provides a kind of ultrasonic wave microorganism diverting device, and Fig. 1 provides the schematic diagram of this ultrasonic wave microorganism diverting device.Described diverting device is made up of ultrasonic generator 1 and ultrasonic transducer 2, during dispersion, by hold the test tube 3 of microbial solution outer wall directly with the end face close contact of ultrasonic transducer 2, particularly, the end face close contact of the direct and ultrasonic transducer 2 in the bottom of test tube 3.Preferably, the end face of ultrasonic transducer 2 can be provided with groove 4, coordinate with the bottom of test tube 3, make the bottom of test tube 3 have at least a border directly to contact with the end face of ultrasonic transducer 2, such dispersion effect is better.
During dispersion, operator hold test tube 3 with hand steered, to play fixed action, ensure the close contact of the end face of itself and ultrasonic transducer 2.
Can be plane or cambered surface various ways bottom test tube 3.In addition test tube 4 also can be other containers such as beaker.
The type of ultrasonic transducer 2 is also not particularly limited, and capable field technique personnel match as required.Can make tubaeform, cylindrical various shape such as grade.The frequency of transverter and power etc. according to treating that the sample size of dispersed sample and sample type adjust accordingly, be such as mycobacterium tuberculosis for sample, the frequency of the ultrasonic transducer that those skilled in the art preferably adopt is 20KHz ~ 80KHz, and power is 5W ~ 200W.Wherein 2-a is the sample schematic diagram before dispersion, and 2-b dilutes 10 after ultrasonic disperse -1sample drawing doubly, 2-c adopts the method for plate culture count to carry out counting statistics, dilution 10 -6doubly, 37 DEG C of constant incubators cultivate the sample drawing after 2 days.Under equal conditions, adopt liquid-transfering gun pressure-vaccum mode of averaging to carry out the dispersion of mycobacterium tuberculosis, also adopt the processing mode identical with the sample after ultrasonic disperse, as shown in Figure 3, wherein 3-a is the sample schematic diagram before dispersion to result, and 3-b dilutes 10 after ultrasonic disperse -1sample drawing doubly, 3-c adopts the method for plate culture count to carry out counting statistics, dilution 10 -6doubly, 37 DEG C of constant incubators cultivate the sample drawing after 2 days.Can be found by contrast, under similarity condition, sample after ultrasonic disperse, be uniformly dispersed, agglomerating few, dispersion effect is obviously good than the mechanical dispersion effect of tradition, and the result of the method for plate culture count counting also shows, bacterial growth after ultrasonic disperse is good, and bacterium number is more, does not also affect after ultrasonic disperse is described on the activity of bacterium.
Further, diverting device can arrange a stationary installation, fixed container during for disperseing, and ensures the close contact of the end face of itself and ultrasonic transducer, handled easily.
Above better embodiment of the present utility model is illustrated, but the invention is not limited to described embodiment, those of ordinary skill in the art also can make all equivalent modifications or replacement under the prerequisite without prejudice to the utility model spirit, and these equivalent modification or replacement are all included in the application's claim limited range.

Claims (5)

1. a ultrasonic wave microorganism diverting device, is characterized in that: described diverting device comprises ultrasonic generator and ultrasonic transducer, is electrically connected between described ultrasonic generator and ultrasonic transducer; Described diverting device also comprises a container, and for holding microorganism to be disperseed, described container can move freely; The outer wall of described container and the end face close contact of described ultrasonic transducer.
2. diverting device according to claim 1, is characterized in that: the bottom of described container and the end face close contact of described ultrasonic transducer.
3. diverting device according to claim 2, is characterized in that: the end face of described ultrasonic transducer has a groove, coordinates with the bottom of described container, makes described container bottom have at least a border and ultrasonic transducer end face to have close contact.
4. diverting device according to claim 1, is characterized in that, described diverting device also comprise one for disperseing time fix the stationary installation of described container.
5. the diverting device according to any one of claim 1-4, is characterized in that, the frequency of the ultrasonic transducer of described diverting device is 20KHz ~ 80KHz, and power is 5W ~ 200W.
CN201420560535.XU 2014-09-26 2014-09-26 A kind of ultrasonic wave microorganism diverting device Active CN204138668U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201420560535.XU CN204138668U (en) 2014-09-26 2014-09-26 A kind of ultrasonic wave microorganism diverting device

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Application Number Priority Date Filing Date Title
CN201420560535.XU CN204138668U (en) 2014-09-26 2014-09-26 A kind of ultrasonic wave microorganism diverting device

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CN204138668U true CN204138668U (en) 2015-02-04

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104263718B (en) * 2014-09-26 2017-11-24 广东体必康生物科技有限公司 A kind of ultrasonic wave microorganism process for dispersing and device

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104263718B (en) * 2014-09-26 2017-11-24 广东体必康生物科技有限公司 A kind of ultrasonic wave microorganism process for dispersing and device

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GR01 Patent grant
C41 Transfer of patent application or patent right or utility model
TR01 Transfer of patent right

Effective date of registration: 20151222

Address after: 528000 Guangdong Province, Foshan city Chancheng District ancient road No. 70 Foshan high tech Zone Science and Technology Industrial Park C block 101, 102, 108

Patentee after: Guangdong skyWork Biotechnology Co. Ltd.

Address before: 1 No. 528000 Guangdong city of Foshan province Foshan City Hing Road

Patentee before: GUANGDONG TIBIKANG BIOLOGICAL SCIENCE & TECHNOLOGY CO., LTD.