CN204107093U - A kind of segmented cylinder chromatographic apparatus - Google Patents
A kind of segmented cylinder chromatographic apparatus Download PDFInfo
- Publication number
- CN204107093U CN204107093U CN201420370838.5U CN201420370838U CN204107093U CN 204107093 U CN204107093 U CN 204107093U CN 201420370838 U CN201420370838 U CN 201420370838U CN 204107093 U CN204107093 U CN 204107093U
- Authority
- CN
- China
- Prior art keywords
- chromatographic column
- segmentation
- chromatographic
- reservoir
- segmented cylinder
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Treatment Of Liquids With Adsorbents In General (AREA)
Abstract
The utility model provides a kind of segmented cylinder chromatographic apparatus, comprise reservoir, segmentation chromatographic column, connect liquid bottle and trestle table, the below connection segment chromatographic column of reservoir, for connecing liquid bottle below segmentation chromatographic column liquid outlet, described in connect liquid bottle be placed on can support reservoir and segmentation chromatographic column trestle table on; Reservoir is constant pressure funnel, is provided with the second piston, and lower end is connected with segmentation chromatographic column is airtight, and upper end is provided with funnel plug.This segmented cylinder chromatographic apparatus that the utility model relates to, assembling flexibly, can realize automatic constant speed liquid feeding, can be separated by wash-out for a long time, can change chromatographic column height, economical and practical, easy to operate, can enhance productivity again.
Description
Technical field
The utility model relates to a kind of segmented cylinder chromatographic apparatus, comprises reservoir, segmentation chromatographic column, connects liquid bottle and trestle table.
Background technology
Column chromatography for separation process is filled in chromatographic column by column packing, then with a certain proportion of elution band separator.By changing the polarity of eluent, different material being separated, and elutes sequentially, thus reaching the effect of separation and purification.In polysaccharide medicine developmental research, often can use post separation method.But because conventional column packing (glucan, agarose etc.) particle diameter is less, density is comparatively large, the polysaccharide high molecular polymer making viscosity large is comparatively slow in the radial diffusion of chromatographic column, is separated consuming time longer.Constantly slow liquid feeding is needed during manual operation.Adopt pressurization method can increase work efficiency to a certain extent, but due to the rigidity of this kind of column packing poor, very easily pressurization process in damage filler, also can increase chromatographic column damage danger coefficient.Preparative liquid chromatography system can comparatively accurate controlled pressure, but the more difficult coupling of different size chromatographic column of experiment demand, and involve great expense.Therefore, need one can long-time, constant elution flow rate in the separation of polysaccharides research of laboratory, again can flexible assembling different size chromatographic column, economical and practical tomographic system.
Utility model content
The utility model provides a kind of segmented cylinder chromatographic apparatus, can carry out the chromatographic apparatus of efficient separation and purification to polysaccharide high molecular polymer.
For solving the above technical problem, the technical scheme that the utility model provides is as follows: a kind of segmented cylinder chromatographic apparatus, comprise reservoir, segmentation chromatographic column, connect liquid bottle and trestle table, the below connection segment chromatographic column of described reservoir, for connecing liquid bottle below described segmentation chromatographic column liquid outlet, described in connect liquid bottle be placed on can support reservoir and segmentation chromatographic column trestle table on; Reservoir is constant pressure funnel, is provided with the second piston, and lower end is connected with segmentation chromatographic column is airtight, and upper end is provided with funnel plug.
Further, segmentation chromatographic column comprises the first chromatographic column and the second chromatographic column, the built-in sieve plate of described first chromatographic column, and bottom connects first piston, and top is provided with two groove built-in rubber sealing rings, is connected with the second chromatographic column is airtight by chromatographic column sleeve pipe.
Reservoir liquid outlet is provided with the second piston.Before experiment, rotate the second piston to closed condition, open funnel plug, pour appropriate eluent into, during column chromatography wash-out, rotate the second piston and regulate eluent flow rate, can realize reservoir internal pressure constant, eluent constant speed flows out.
This segmented cylinder chromatographic apparatus that the utility model relates to, flexible assembling is needed to become the chromatographic column of different size according to chromatography, and automatic constant speed liquid feeding can be realized, can be separated by wash-out for a long time, and flexible assembling changes chromatographic column height, economical and practical, easy to operate, can enhance productivity again.
Accompanying drawing explanation
Fig. 1 is overall structure figure of the present utility model;
Fig. 2 is the cross-sectional view of the utility model segmentation chromatographic column;
Fig. 3 is the longitudinal section of the utility model segmentation chromatographic column and chromatographic column sleeve pipe;
Wherein, 1----reservoir, 2----segmentation chromatographic column, 3----connect liquid bottle, 4----trestle table, 11----funnel plug, 12----second piston, 21----first chromatographic column, 22----second chromatographic column, 23----chromatographic column sleeve pipe, 24----rubber seal, 25----sieve plate, 26----first piston, 27----groove I, 28----groove II, 29----chromatographic column inner chamber, 41----pulley, 42----down fixing rack.
Detailed description of the invention
As depicted in figs. 1 and 2, segmented cylinder chromatographic apparatus comprises reservoir 1, segmentation chromatographic column 2, connects liquid bottle 3 and trestle table 4, described reservoir 1 is connected with described segmentation chromatographic column 2, and described segmentation chromatographic column 2 liquid outlet stretches into and connects in liquid bottle 3, connects liquid bottle 3 and is placed in trestle table 4; Reservoir 1 and segmentation chromatographic column 2 shaft are equipped with scale; Be provided with multiple roller 41 below trestle table 4, top connects down fixing rack 42, and connected mode is bolt and connects; Down fixing rack 42 be provided with a joint pin and be multiplely movably connected on joint pin, horizontally disposed geometrical clamp, geometrical clamp clamps reservoir and segmentation chromatographic column respectively; Described reservoir upper portion side wall connects overflow pipe, the tail end connection segment chromatographic column of overflow pipe.
Wherein, reservoir 1 is a constant pressure funnel, and material is clear glass, and device body has volume scale, accurately can weigh the volume of eluent.
Chromatographic column sleeve pipe 23 inwall two ends are respectively equipped with two groove I27 and dock with chromatographic column unit with grooves in outer wall II28, are sealed by built-in rubber seal 24, and chromatographic column sleeve pipe 23 middle part lumen diameter is equal with chromatographic column internal diameter.
As shown in figures 1 and 3, segmentation chromatographic column 2 material is transparent poly-third vinyl plastics, the first built-in sieve plate 25 in chromatographic column 21 lower end, and liquid outlet is provided with first piston 26, and the first chromatographic column upper end outer wall has two circle groove II28; Second chromatographic column 22 is the cylindric of both ends open, and upper and lower side outer wall respectively has two circle groove II28; Chromatographic column sleeve pipe 23 is the cylindric of both ends open, and upper and lower side inwall respectively has two circle groove I27.Two groove I27 can built-in rubber sealing ring 24, and then realizes two airtight docking of chromatographic column.Second chromatographic column (22) is segmentation structure, intersegmental employing rubber seal is divided to be connected with chromatographic column bobbin seal, its Hermetical connecting structure is identical with aforesaid way, rubber seal plays the effect of sealing on the one hand, the extruding force between rubber seal and chromatographic column sleeve pipe and frictional force can be increased on the other hand, the various piece of the segmentation chromatographic column utilizing chromatographic column sleeve pipe to connect is connected tightr, this structure is easy to installation and removal, is also easy to the parts installing and change segmentation chromatographic column inside.
The utility model also experimentally can need the chromatographic column being assembled into different length.Chromatographic column shaft is provided with scale, accurately can weigh the volume of column packing and eluent.First piston 26 can be realized equal with the second piston 12 place flow rate of liquid in column chromatography elution process.
As shown in Figure 1, have pulley 41 below trestle table 4, there is down fixing rack 42 top, is all fixedly connected with trestle table 4 by bolt or screw.Reservoir 1 and segmentation chromatographic column 2 is fixed with geometrical clamp.With 4 pulleys 41, the movement of tomographic system can be facilitated.All with brake on pulley, by regulating brake fixed support platform position when carrying out column chromatography wash-out, make experimental implementation more convenient, result is more accurate.
The using method of above-mentioned segmented cylinder chromatographic apparatus, comprise following step: step one, preliminary preparation, dry after segmentation chromatographic column 2 is cleaned up, need to be assembled into length-specific according to experiment, close first piston 26, the filler prepared (Sephadex G100) is packed in segmentation chromatographic column 2, to connect liquid bottle 3 is placed in immediately below segmentation chromatographic column 2 liquid outlet, according to the height connecing liquid bottle 3 and adjust segmentation chromatographic column 2, with geometrical clamp, the segmentation chromatographic column 2 filling filler is vertically fixed on down fixing rack 42, compound (polysaccharide) solution of purifying to be separated is added in segmentation chromatographic column 2.Be connected with segmentation chromatographic column 2 is airtight by reservoir 1, close the second piston 12, in segmented cylinder chromatographic apparatus, add appropriate eluent (water) stand-by; Step 2, automatic liquid-feeding separation and purification, open the second piston 12 and first piston 26 successively, regulates eluting liquid flow velocity, can realize automatic liquid-feeding function; Step 3, follow-up work, treat chromatography cross post complete after, close first piston 26 and the second piston 12, the product after purifying sealed up for safekeeping, and by reservoir 1, segmentation chromatographic column 2 with connect liquid bottle 3 and clean up.
Detailed description of the invention described in the utility model does not form the restriction to the application's scope; within every spirit in the utility model design and principle, any amendment that one of skill in the art can make, equivalent to replace and improvement etc. all should be included within protection domain of the present utility model.
Claims (9)
1. a segmented cylinder chromatographic apparatus, it is characterized in that, described chromatographic apparatus comprises reservoir (1), segmentation chromatographic column (2), connects liquid bottle (3) and trestle table (4), below connection segment chromatographic column (2) of described reservoir (1), for connecing liquid bottle below described segmentation chromatographic column (2) liquid outlet, described in connect liquid bottle (3) be placed on can support reservoir and segmentation chromatographic column trestle table (4) on.
2. a kind of segmented cylinder chromatographic apparatus according to claim 1, it is characterized in that, described reservoir (1) is constant pressure funnel, is provided with the second piston (12), and lower end is connected with segmentation chromatographic column is airtight, and upper end is provided with funnel plug (11).
3. a kind of segmented cylinder chromatographic apparatus according to claim 1 and 2, it is characterized in that, described segmentation chromatographic column (2) comprises the first chromatographic column (21) and the second chromatographic column (22), the built-in sieve plate of described first chromatographic column (21) (25), bottom connects first piston (26), top is provided with two groove I built-in rubber sealing rings, is connected with the second chromatographic column (22) is airtight by chromatographic column sleeve pipe (23).
4. a kind of segmented cylinder chromatographic apparatus according to claim 3, it is characterized in that: described chromatographic column sleeve pipe (23) inwall two ends are respectively equipped with two grooves and segmentation chromatography column outer wall groove II(28) dock, by the sealing of built-in rubber seal (24), chromatographic column sleeve pipe (23) middle part lumen diameter is equal with segmentation chromatography column internal diameter.
5. a kind of segmented cylinder chromatographic apparatus according to claim 1, is characterized in that: described reservoir (1) and segmentation chromatographic column (2) shaft are equipped with scale.
6. a kind of segmented cylinder chromatographic apparatus according to claim 1, is characterized in that: described trestle table (4) below is provided with multiple roller (41), and top connects down fixing rack (42), and connected mode is bolt and connects.
7. a kind of segmented cylinder chromatographic apparatus according to claim 6, it is characterized in that: described down fixing rack (42) be provided with a joint pin and be multiplely movably connected on joint pin, horizontally disposed geometrical clamp, geometrical clamp clamps reservoir and segmentation chromatographic column respectively.
8. a kind of segmented cylinder chromatographic apparatus according to claim 1, is characterized in that: described reservoir upper portion side wall connects overflow pipe, the tail end connection segment chromatographic column of overflow pipe.
9. a kind of segmented cylinder chromatographic apparatus according to claim 3, is characterized in that: described second chromatographic column (22) is segmentation structure, and a point intersegmental employing rubber seal is connected with chromatographic column bobbin seal.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420370838.5U CN204107093U (en) | 2014-07-07 | 2014-07-07 | A kind of segmented cylinder chromatographic apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420370838.5U CN204107093U (en) | 2014-07-07 | 2014-07-07 | A kind of segmented cylinder chromatographic apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204107093U true CN204107093U (en) | 2015-01-21 |
Family
ID=52324456
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420370838.5U Expired - Fee Related CN204107093U (en) | 2014-07-07 | 2014-07-07 | A kind of segmented cylinder chromatographic apparatus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN204107093U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105771314A (en) * | 2016-04-26 | 2016-07-20 | 浙江万里学院 | Combined column chromatography system and use method thereof |
| CN108771968A (en) * | 2018-06-14 | 2018-11-09 | 河海大学 | A variety of isotope ion separation column devices and separation method |
-
2014
- 2014-07-07 CN CN201420370838.5U patent/CN204107093U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105771314A (en) * | 2016-04-26 | 2016-07-20 | 浙江万里学院 | Combined column chromatography system and use method thereof |
| CN108771968A (en) * | 2018-06-14 | 2018-11-09 | 河海大学 | A variety of isotope ion separation column devices and separation method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN204107093U (en) | A kind of segmented cylinder chromatographic apparatus | |
| CN102895798B (en) | Simple semi-automatic chromatographic column | |
| CN207101968U (en) | A kind of chromatography column apparatus of automatic liquid-feeding | |
| CN201115795Y (en) | Novel chromatographic column | |
| CN202136832U (en) | Normal pressure column chromatography device under closed environment | |
| CN203916148U (en) | A kind of pressurization constant temperature chromatographic column | |
| CN203440269U (en) | Chromatography device for purified plasma proteins | |
| CN203678012U (en) | Splittable chromatographic column | |
| CN106290669A (en) | Liquid chromatographic system flowing is added and expanded in volume device mutually | |
| CN204364947U (en) | SPE auxiliary piping system | |
| CN203954734U (en) | The column chromatography combination unit that the adjustable column temperature of a kind of efficient dress post can freely break | |
| CN207153163U (en) | A kind of combined type column chromatography device | |
| CN204815785U (en) | Automatic control chromatographic device | |
| CN208049452U (en) | Column chromatography amplification technique equipment | |
| CN205719799U (en) | A kind of without shuttle valve type minor diameter seamless steel tube hydrostatic testing machine | |
| CN202315412U (en) | Constant-pressure automatic liquid-adding chromatography device | |
| CN207755812U (en) | A kind of chromatographic column of constant pressure chromatography | |
| CN208630946U (en) | A kind of filtering conveying device of organosilicon sealant | |
| CN208081920U (en) | A kind of automatic liquid supply chromatographic column | |
| CN202453332U (en) | High performance liquid-phase preparative chromatographic column | |
| CN207630137U (en) | A kind of clamping device of plastic pipe cutting machine | |
| CN102068832A (en) | Normal pressure column chromatography device under closed environment | |
| CN108744594A (en) | It is a kind of it is novel can automatic feed liquor chromatographic apparatus | |
| CN203598535U (en) | Device convenient to switch flow path direction of Streamline column | |
| CN207861865U (en) | A kind of novel material dropwise addition equipment |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150121 Termination date: 20150707 |
|
| EXPY | Termination of patent right or utility model |