CN203916148U - A kind of pressurization constant temperature chromatographic column - Google Patents
A kind of pressurization constant temperature chromatographic column Download PDFInfo
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- CN203916148U CN203916148U CN201420328598.2U CN201420328598U CN203916148U CN 203916148 U CN203916148 U CN 203916148U CN 201420328598 U CN201420328598 U CN 201420328598U CN 203916148 U CN203916148 U CN 203916148U
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Abstract
The utility model discloses a kind of pressurization constant temperature chromatographic column, comprise cylinder, eluent feeder and post lid, post lid is connected in end entrance on eluent feeder, and the outlet of eluent feeder lower end is connected with end entrance on cylinder, it is characterized in that, described cylinder is provided with constant temperature jacket; In described eluent feeding device, be provided with liquid distribution trough, and be provided with the safety valve with piston on eluent feeding device, and duplex ball connector.On described liquid distribution trough, be uniformly distributed one group of aperture, top arranges flow control valve I; Described damaged surface is provided with scale, and lower end is provided with core, and delivery channel place is provided with flow control valve II; Described post lid, cylinder and eluent feeder all adopt standard frosted to be threaded mouthful to be connected.Pressurization constant temperature chromatographic column of the present utility model, can balance chromatographic column external and internal pressure when supplementing eluent, and constant temperature jacket can key-course eutectoid temperature, and avoids the irregular impact in adsorbent top layer.
Description
Technical field
The utility model relates to a kind of chromatographic column, particularly relates to a kind of pressurization constant temperature chromatographic column, and chromatographic column is used for extracting separation chemistry product and natural products, belongs to extraction separator technical field biological, chemical field.
Background technology
Column chromatography is in the chromatographic column of filled with adsorbent, utilizes same adsorbent different to each component adsorption capacity in sample, and in elution process, each component is because of the different isolation technics of separating gradually of translational speed.In order to make the separation better of each component, often use the method for eluent gradient elution to carry out column chromatography, first use the eluent that polarity is little to carry out wash-out, strengthen gradually the content of the component that eluent Semi-polarity is larger.But use the less eluent of polarity, the process time that adsorbs-free is longer, required time of column chromatography is also longer.Adopt the mode of pressurization to carry out column chromatography, can greatly shorten experimental period., mainly there is following defect in existing pressurizing chromatographic column: when supplementing new eluent, can not balance columns external and internal pressure, and often cause chromatographic column lid and fly to burst out, there is potential safety hazard; When conventional chromatographic column eluent is injected by cylinder center, easily impact the adsorbent on top layer, affected the effect of post separation; Chromatographic column temperature lacks adjusting, and different room temperatures in season differ greatly, and the separating effect of column chromatography is had to impact.
Summary of the invention
The purpose of this utility model is a kind of pressurization constant temperature chromatographic column proposing, and it can effectively overcome the shortcoming of existing chromatographic column, makes column chromatography experiment be able to efficient, safety, complete reliably.
For addressing the above problem, the utility model is taked following technical scheme:
Described pressurization constant temperature chromatographic column, comprises cylinder, eluent feeder and post lid, and post lid is connected in end entrance on eluent feeder, and the outlet of eluent feeder lower end is connected with end entrance on cylinder, it is characterized in that, described cylinder is provided with constant temperature jacket; In described eluent feeding device, be provided with liquid distribution trough, and be provided with the safety valve with piston on eluent feeding device, and duplex ball connector.
Preferably, described post lid adopts supporting standard frosted connector to be connected with eluent feeder, and internal diameter is chosen as 19mm; The outlet of described eluent feeder and cylinder entrance adopt the supporting standard frosted mouth that is threaded to be connected, and internal diameter is chosen as 19mm.
Preferably, described eluent feeding device comprises flow control valve I, is arranged on liquid distribution trough top; On described liquid distribution trough, be uniformly distributed one group of aperture, hole diameter is chosen as 3mm; Safety valve internal diameter is chosen as 5mm, and safety valve is provided with piston; Duplex ball connector on described eluent feeding device, internal diameter is chosen as 5mm.
Preferably, the constant temperature jacket on described chromatographic column cylinder, the optional internal diameter 30mm of chuck, external diameter 50mm, the internal diameter of constant temperature jacket water inlet and delivery port is 5mm.
Preferably, to be provided with minimum division value be 2mm scale to described damaged surface; Filled with adsorbent in described chromatographic column cylinder, lower end is provided with core, the optional 3mm of core thickness; Described chromatographic column delivery channel place is provided with flow control valve II, and the optional internal diameter of delivery channel is 8mm, length 80mm.
The beneficial effects of the utility model comprise: (1) is provided with the scale that minimum division value is 2mm above cylinder, can conveniently read the packed height of adsorbent, and can calculate comparatively accurately flow velocity according to the falling head of eluent; (2) lower end of cylinder is provided with core, can prevent the aperture of adsorbent choked flow control valve II; (3) the long duct place of cylinder is provided with flow control valve II, can effectively control the flowing velocity of eluent; (4) in eluent feeding device, be provided with liquid distribution trough, make eluent evenly inject lentamente cylinder, avoid the adsorbent top layer out-of-flatness of filling; (5) eluent feeding device is provided with safety valve, when needs supplement eluent, can open the safety-valve, and makes chromatographic column internal and external pressure balance; (6) post lid, eluent feeder and cylinder adopt supporting standard frosted to be threaded mouthful to be connected, and while preventing from pressurizeing, eluent feeder or post cover and fly to burst out, and experimental implementation is safer; (7) cylinder is provided with chuck, can key-course eutectoid temperature, prevent that different room temperatures from having impact to chromatography.
Accompanying drawing explanation
Fig. 1 is the structural representation of pressurization constant temperature chromatographic column of the present utility model.
In figure: 1-post lid, 2-flow control valve I, 3-safety valve, the frosted of 4-standard are threaded mouthful, 5-band scale cylinder, 6-constant temperature jacket water inlet, 7-core, 8-flow control valve II, 9-eluent feeder, 10-liquid distribution trough, 11-duplex ball connector, 12-constant temperature jacket delivery port.
The specific embodiment
Below in conjunction with drawings and Examples, the technical solution of the utility model is described in further detail.
As shown in Figure 1, a pressurization constant temperature chromatographic column, it comprises: 1-post lid, 2-flow control valve I, 3-safety valve, the frosted of 4-standard are threaded mouthful, 5-band scale cylinder, 6-constant temperature jacket water inlet, 7-core, 8-flow control valve II, 9-eluent feeder, 10-liquid distribution trough, 11-duplex ball connector and 12-constant temperature jacket delivery port.
Post lid 1 is connected in eluent feeder 9 upper ends, and the 9 lower end outlets of eluent feeder are connected with end entrance on cylinder 5.Described post lid 1 adopts supporting standard frosted connector to be connected with eluent feeder 9, and internal diameter is 19mm; Described eluent feeder and cylinder adopt supporting standard frosted to be threaded mouthfuls 4 to be connected, and internal diameter is 19mm.
Described cylinder 5 is provided with constant temperature jacket, can pass into recirculated water, prevents that different room temperatures from having impact to column chromatography separating effect.Described chromatographic column chuck internal diameter 30mm, external diameter 50mm; Described cylinder length 500mm; The water inlet 6 of described chromatographic column cylinder 5 constant temperature jackets is 5mm with the internal diameter of delivery port 12.
Described cylinder 5 surfaces are provided with the scale that minimum division value is 2mm, can conveniently read the packed height of adsorbent, and can calculate comparatively accurately flow velocity according to the falling head of eluent.
The interior filled with adsorbent of described cylinder 5, lower end is provided with core 7, and core 7 thickness 3mm can prevent the aperture of adsorbent choked flow control valve II 8.The delivery channel place of described cylinder 5 is provided with flow control valve II 8, and catheter diameter is 8mm, and length 80mm can effectively control the flowing velocity of eluent.
Described eluent feeding device 9 height 200mm, adopt supporting standard frosted to be threaded and mouthful are connected with cylinder 5, and while preventing from pressurizeing, eluent feeder 9 flies to burst out, and experimental implementation is safer.In described eluent feeding device, be provided with liquid distribution trough 10, make eluent inject uniformly and effectively cylinder, avoid filler surface irregularity.On described liquid distribution trough 10, be uniformly distributed one group of aperture, small aperture is 3mm.Liquid distribution trough 10 tops arrange flow control valve I 2.
On described eluent feeding device 9, be also provided with safety valve 3, internal diameter is 5mm, when needs supplement eluent, can open the safety-valve 3, makes chromatographic column internal and external pressure balance.Described eluent feeding device is provided with duplex ball connector 11, and internal diameter is 5mm.
During use, first clean up package unit and in oven for drying, shut the piston of flow control valve II 8, after filled with adsorbent, add sample.Load onto afterwards eluent feeder 9, shut the piston of safety valve 3 and flow control valve I 2, connect duplex ball.
While starting column chromatography, open the piston of flow control valve I 2, until filling with in chromatographic column after eluent, shut the piston of flow control valve I 2, open the piston of flow control valve II 8.Start, with the pressurization of duplex ball, to accelerate the speed of column chromatography.When if the liquid level of eluent approaches adsorbent top layer, first shut the piston of flow control valve II 8,3 piston opens the safety-valve, make post internal and external pressure balance, shut afterwards the piston of safety valve 3, open the piston of flow control valve I 2, add eluant, eluent, continue column chromatography.
The above, be only preferred embodiment of the present utility model, not the utility model done to any pro forma restriction, although the utility model is with preferred embodiment exposure as above, yet not in order to limit the utility model.Any those skilled in the art, are not departing within the scope of the utility model, can carry out various conversion and be equal to replacement the utility model patent.Therefore, the utility model is not limited to the disclosed specific embodiment, and should comprise the whole embodiments that fall within the scope of the utility model Patent right requirement.
Claims (6)
1. a pressurization constant temperature chromatographic column, comprises cylinder, eluent feeder and post lid, and post lid is connected in end entrance on eluent feeder, and the outlet of eluent feeder lower end is connected with end entrance on cylinder, it is characterized in that: described cylinder is provided with constant temperature jacket; In described eluent feeding device, be provided with liquid distribution trough, and be provided with the safety valve with piston on eluent feeding device, and duplex ball connector.
2. pressurization constant temperature chromatographic column according to claim 1, is characterized in that: described post lid adopts supporting standard frosted connector to be connected with eluent feeder; Described eluent feeder and cylinder adopt supporting standard frosted to be threaded mouthful to be connected.
3. pressurization constant temperature chromatographic column according to claim 1, is characterized in that: on described liquid distribution trough, be uniformly distributed one group of aperture.
4. pressurization constant temperature chromatographic column according to claim 1, is characterized in that: eluent feeding device is provided with flow control valve I, is arranged on liquid distribution trough top.
5. pressurization constant temperature chromatographic column according to claim 1, is characterized in that: filled with adsorbent in described cylinder, and lower end is provided with core, and delivery channel place is provided with flow control valve II.
6. pressurization constant temperature chromatographic column according to claim 1, is characterized in that: described damaged surface is provided with scale.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420328598.2U CN203916148U (en) | 2014-06-19 | 2014-06-19 | A kind of pressurization constant temperature chromatographic column |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420328598.2U CN203916148U (en) | 2014-06-19 | 2014-06-19 | A kind of pressurization constant temperature chromatographic column |
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| CN203916148U true CN203916148U (en) | 2014-11-05 |
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| CN201420328598.2U Expired - Fee Related CN203916148U (en) | 2014-06-19 | 2014-06-19 | A kind of pressurization constant temperature chromatographic column |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104941253A (en) * | 2015-05-28 | 2015-09-30 | 安徽中医药大学 | Column temperature controlling high borosilicate glass interlayer chromatography column |
| CN105771314A (en) * | 2016-04-26 | 2016-07-20 | 浙江万里学院 | Combined column chromatography system and use method thereof |
| CN113466081A (en) * | 2021-08-09 | 2021-10-01 | 西北大学 | Method for efficiently determining four components of coal tar |
| CN115524432A (en) * | 2022-09-22 | 2022-12-27 | 内蒙古仓颉科技有限公司 | Intelligent sample separation system of chromatographic analyzer |
-
2014
- 2014-06-19 CN CN201420328598.2U patent/CN203916148U/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104941253A (en) * | 2015-05-28 | 2015-09-30 | 安徽中医药大学 | Column temperature controlling high borosilicate glass interlayer chromatography column |
| CN105771314A (en) * | 2016-04-26 | 2016-07-20 | 浙江万里学院 | Combined column chromatography system and use method thereof |
| CN113466081A (en) * | 2021-08-09 | 2021-10-01 | 西北大学 | Method for efficiently determining four components of coal tar |
| CN115524432A (en) * | 2022-09-22 | 2022-12-27 | 内蒙古仓颉科技有限公司 | Intelligent sample separation system of chromatographic analyzer |
| CN115524432B (en) * | 2022-09-22 | 2023-10-20 | 内蒙古仓颉科技有限公司 | Chromatographic analyzer intelligent sample separation system |
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| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20141105 Termination date: 20170619 |