CN108744594A - It is a kind of it is novel can automatic feed liquor chromatographic apparatus - Google Patents
It is a kind of it is novel can automatic feed liquor chromatographic apparatus Download PDFInfo
- Publication number
- CN108744594A CN108744594A CN201810783857.3A CN201810783857A CN108744594A CN 108744594 A CN108744594 A CN 108744594A CN 201810783857 A CN201810783857 A CN 201810783857A CN 108744594 A CN108744594 A CN 108744594A
- Authority
- CN
- China
- Prior art keywords
- spherical
- chromatographic column
- main body
- ball portion
- novel
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/12—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the preparation of the feed
- B01D15/125—Pre-filtration
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/14—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the introduction of the feed to the apparatus
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/20—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the sorbent material
- B01D15/203—Equilibration or regeneration
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/22—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the construction of the column
Landscapes
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
Abstract
The present invention disclose it is a kind of it is novel can automatic feed liquor chromatographic apparatus, including chromatographic column, spherical geat container, speed-regulating type peristaltic pump and force (forcing) pump, the spherical shape geat container includes spherical main body and lower end closed and the spherical hopper pipe affixed with spherical main body, the spherical hopper pipe includes oval ball portion and the cylindrical tube that oval ball portion upper end is connected with spherical main body, the ellipse ball portion lower end closed, it is provided with the identical fluid hole of multiple sizes on the ellipse ball portion ball wall, it is connected with Y-type three way type connector at the top of the spherical shape main body, the chromatographic column is inserted into the cylindrical tube lower end.Eluant, eluent is added in chromatographic column by fluid hole, can prevent plus eluant, eluent damages Silica Surface；Solve the problems, such as that elution rate is slow by force (forcing) pump；Eluant, eluent can be accurately added to spherical geat container by speed-regulating type peristaltic pump, eluant, eluent need not be manually continually added into chromatographic column, save manpower.
Description
Technical field
The present invention relates to column chromatography technology field, more particularly, to it is a kind of it is novel can automatic feed liquor chromatographic apparatus.
Background technology
Chromatographic technique is also known as Column chromatography techniques, and cardinal principle is according to each component in sample mixture in stationary phase and flowing
Distribution coefficient is different in phase, separates component through repeatedly distributing repeatedly.It includes column chromatography, paper chromatography, thin-layer chromatography, height
Imitate liquid chromatography etc..Currently, column chromatography technology is natural products laboratory, chemical laboratory, biology laboratory and related work
The essential common important technology of mixture is isolated and purified in industry production, column chromatography includes mainly normal pressure column chromatography, decompression
Column chromatography and pressurized column chromatography.And normal pressure column chromatography is wherein simplest column chromatography, has easy to operate simple, experiment condition
It is required that being easy to get, the features such as separative efficiency is high, thus be widely used.
Chinese CN201720131404.3 patents disclose a kind of constant pressure device for pressurized column chromatography, including chromatographic column, spherical shape
Geat container and plastic interface folder, spherical geat container bottom, which brings out liquid mouthpiece and inserts, to be fixed in chromatographic column open top, plastics
Interface is fixedly clamped in spherical geat container and chromatographic column junction, and the top center of spherical geat container is provided with note
Liquid mouth and sealing-plug is provided at liquid injection port, the top-side of spherical geat container, which is provided with, to be connect gas port and described meet gas port company
Constant pressure air pump is connect, is provided with cock at chromatographic column bottom end outlet, which is flexibly connected by setting with chromatographic column
Spherical geat container improves the amount of chromatographic column storage eluant, eluent, but in chromatographic column plus is unfolded by spherical geat container
When agent, is directly dripped from top, certain destruction can be caused to the Silica Surface in chromatographic column, for example make Silica Surface portion
Divide recess etc., chromatographic effect is bad when solvent is spread along the Silica Surface of this out-of-flatness.
Invention content
In response to the problems existing in the prior art, the purpose of the present invention is to provide it is a kind of it is novel can automatic feed liquor chromatography dress
It sets.
To achieve the above object, the technical solution adopted by the present invention is：
It is a kind of it is novel can automatic feed liquor chromatographic apparatus, including chromatographic column, spherical geat container, speed-regulating type peristaltic pump and add
Press pump, the spherical shape geat container includes spherical main body and the spherical hopper pipe affixed with spherical main body, the spherical hopper pipe
Including the cylindrical tube that oval ball portion and oval ball portion upper end are connected with spherical main body, the ellipse ball portion lower end closed,
It is provided with the identical fluid hole of multiple sizes on the ball wall of the ellipse ball portion, Y types three are connected at the top of the spherical shape main body
Leading to connector, the speed-regulating type peristaltic pump and the force (forcing) pump are connected by pipeline with Y-type three way type connector both ends respectively,
The both ends that the Y-type three way type connector is connect with the speed-regulating type peristaltic pump and the force (forcing) pump are equipped with valve；The spherical shape
Main body bottom wall is abutted with the chromatographic column upper end, and the cylindrical tube upper end is connected to the spherical main body, the spherical hopper pipe
The chromatographic column is inserted into lower end.
It when using technical solution of the present invention, directly falls in silica gel upper surface compared to eluant, eluent and causes to be recessed, leaked by spherical shape
Fluid hole on the ball wall of the oval ball portion of bucket pipe adds eluant, eluent in chromatographic column, and eluant, eluent can be sprayed along fluid hole be flushed to
It chromatographs on post jamb, Silica Surface is flowed to along post jamb, can prevent plus eluant, eluent damages Silica Surface；Pass through Y-type three way type
Connector connects speed-regulating type peristaltic pump and force (forcing) pump, so that system connection is more stablized, it is slow that force (forcing) pump solves elution rate
Problem；By speed-regulating type peristaltic pump eluant, eluent can be accurately added to spherical geat container automatically, it need not manually frequently
Ground adds eluant, eluent into chromatographic column, saves human and material resources, improves the efficiency of separating-purifying；Spherical main body upper end is set simultaneously
It is equipped with the feed inlet with corking, facilitate the addition of eluant, eluent while eluant, eluent volatilization can be reduced.
Preferably, it is equipped between the chromatographic column and the spherical geat container and sleeve, the height-regulating sleeve and institute is turned up
It states chromatographic column upper end to be connected through a screw thread, the spherical main body bottom wall described at this time is abutted with the height-regulating sleeve upper surface, the ball
Shape filler tube passes through the height-regulating sleeve to be inserted into the chromatographic column；Spherical geat container and layer can be adjusted by adjusting height-regulating sleeve
Analyse column in silica gel upper surface relative altitude, prevent experimenter add in chromatographic column silica gel it is excessively high when, chromatography addition funnel
The sealed end of lower part is contacted with Silica Surface damages Silica Surface.
Preferably, the height-regulating sleeve offers internal thread, and the chromatographic column upper end and the portion that sleeve connection is turned up separate
Equipped with external screw thread；What external screw thread can prevent from being added has destruction of corrosive eluant, eluent to screw thread pair.
Preferably, the fluid hole is distributed between oval ball portion top and bottom in multi-level, each layer of flow liquid
Circumferencial direction of the hole all along oval ball portion ball wall is uniformly distributed；In this way so that being flushed to washing on chromatography post jamb by fluid hole spray
De- agent can along its it is circumferentially uniform be distributed in entire chromatographic column internal face, to which make can along the eluant, eluent that flows down of chromatography post jamb
It is contacted from multiple directions with Silica Surface, such eluant, eluent is more uniform along the infiltration of silica gel, and chromatographic effect is more preferable.
Preferably, the minimum distance of the elliptical shape ball outer wall and the chromatography column wall is two millimeters, so that
Eluant, eluent can preferably be sprayed along fluid hole is flushed to chromatography column wall.
Preferably, the chromatographic column lower port is provided with dismountable sand core, and the sand core lower end is provided with cock, convenient
By sand core disassembly, cleaning, cock can control the switch of chromatography process.
Compared with prior art, the beneficial effects of the invention are as follows：
(1) eluant, eluent is added in chromatographic column by the fluid hole on the oval ball portion ball wall of spherical hopper pipe, eluted
Agent can be sprayed along fluid hole to be flushed on chromatography post jamb, is flowed to Silica Surface along post jamb, can be prevented plus eluant, eluent is to Silica Surface
It damages, while oval ball portion can open up more fluid holes under the premise of keeping spherical hopper pipe stabilized structure,
So that the speed by shape geat container in chromatographic column plus when eluant, eluent is faster, the efficiency of column chromatography is improved；Pass through force (forcing) pump
Solve the problems, such as that elution rate is slow；Eluant, eluent can be accurately added to spherical geat by speed-regulating type peristaltic pump to hold
Device need not manually continually add eluant, eluent into chromatographic column, save human and material resources, improve the efficiency of separating-purifying；Together
When spherical shape main body upper end be provided with Y-type three way type connector, make connection more stablize, facilitate the addition of eluant, eluent, while Y-type three way type
Connector setting valve can reduce eluant, eluent volatilization；
(2) relative distance between the adjustable chromatography addition funnel of sleeve and chromatographic column is turned up, prevents experimenter toward layer
When addition silica gel is excessively high in analysis column, the sealed end of chromatography addition funnel lower part is contacted with Silica Surface causes brokenly Silica Surface
It is bad, increase the scope of application of the present apparatus.
Description of the drawings
Fig. 1 be the present invention it is a kind of it is novel can automatic feed liquor chromatographic apparatus structural schematic diagram；
In figure：1, chromatographic column；2, sleeve is turned up；3, spherical main body；4, sand core；5, cock；6, speed-regulating type peristaltic pump；7, add
Press pump；8, spherical hopper pipe；801, oval ball portion；802, cylindrical tube；803, fluid hole；9, tee connector；10, valve.
Specific implementation mode
Clear, complete description is carried out to the technical solution of various embodiments of the present invention below with reference to attached drawing, it is clear that retouched
The embodiment stated is only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiment of the present invention, originally
Field those of ordinary skill obtained all other embodiment without making creative work, belongs to this hair
Bright protected range.
The present invention is described in further detail below through specific implementation examples and in conjunction with the accompanying drawings.
Embodiment 1
As shown in Figure 1, it is a kind of it is novel can automatic feed liquor chromatographic apparatus, including chromatographic column 1, be threadedly coupled with chromatographic column 1
Height-regulating sleeve 2 and with the sleeve 2 spherical geat container, speed-regulating type peristaltic pump 6 and the force (forcing) pump 7 that are flexibly connected, the ball is turned up
Shape geat container includes spherical main body 3 and the spherical hopper pipe 8 affixed with spherical main body 3, and the spherical hopper pipe 8 includes ellipse
The cylindrical tube 802 that ball portion 801 and oval 801 upper end of ball portion are connected with spherical main body 3,801 lower end of the ellipse ball portion
It closes, the identical fluid hole 803 of multiple sizes, the top of the spherical shape main body 3 is provided on the ball wall of the ellipse ball portion 801
It is connected with Y-type three way type connector 9, the speed-regulating type peristaltic pump 6 and the force (forcing) pump 7 pass through pipeline and the Y-type three way type respectively
9 both ends of connector are connected, the both ends that the Y-type three way type connector 9 is connect with the speed-regulating type peristaltic pump 6 and the force (forcing) pump 7
It is equipped with valve 10；Spherical shape 3 bottom wall of main body is abutted with 1 upper end of the chromatographic column, 802 upper end of the cylindrical tube and the ball
Shape main body 3 is connected to, and 8 lower end of spherical hopper pipe passes through the height-regulating sleeve 2 to be inserted into the chromatographic column 1.
Specifically, the height-regulating sleeve 2 offers internal thread, 1 inside of the chromatographic column is filled with silica gel, the chromatographic column
1 upper end offers external screw thread with the part that sleeve 2 is connect is turned up；What external screw thread can prevent from being added has corrosive eluant, eluent pair
The destruction of screw thread pair.
Specifically, the fluid hole 803 is distributed between 801 top and bottom of oval ball portion in multi-level, totally six layers,
Circumferencial direction of each layer of the fluid hole 803 all along 801 ball wall of oval ball portion is uniformly distributed；In this way so that passing through fluid hole
803 eluant, eluents that are flushed on 1 wall of chromatographic column of spray can along its it is circumferentially uniform be distributed in 1 internal face of entire chromatographic column, to make
The eluant, eluent flowed down along 1 wall of chromatographic column can be contacted from multiple directions with Silica Surface, rather than only one or two sides
To such eluant, eluent is more uniform along the infiltration of silica gel, and chromatographic effect is more preferable.
Specifically, the minimum distance of ellipse 801 outer wall of ball portion and 1 inner wall of the chromatographic column is two millimeters, to
Eluant, eluent is preferably sprayed along fluid hole 803 and is flushed to 1 inner wall of chromatographic column.
Specifically, 1 lower port of the chromatographic column is provided with dismountable sand core 4,4 lower end of the sand core is provided with cock 5,
Facilitate 4 disassembly, cleaning of sand core, cock 5 can control the switch of chromatography process.
It is incited somebody to action specifically, the speed-regulating type peristaltic pump 6 is connected by emulsion tube with the connector of 9 one end of Y-type three way type connector
In eluant, eluent injection spherical shape main body 3, the force (forcing) pump 7 is connected by rubber tube with the connector of 9 other end of Y-type three way type connector
It pressurizes to the spherical main body 3.
When the present invention works, column chromatography silica gel is filled into the silica gel for being mixed with sample in chromatographic column 1 as required first,
The cock 5 positioned at 1 bottom end of chromatographic column is closed while filler, and then spherical geat container is fixedly mounted by the way that sleeve 2 is turned up
On chromatographic column, 801 bottom wall of oval ball portion of the spherical hopper pipe 8 does not contact the silica gel loaded in chromatographic column 1, opens and adjusts
Fast type peristaltic pump 6 opens simultaneously and connect the valve of one end with Y-type three way type connector 9 and washed what is modulated in advance by emulsion tube
De- agent is automatically added to the spherical main body 3 of spherical geat container, and eluant, eluent passes through the elliptical shape ball connected with spherical main body 3
The circumferential equally distributed spray of fluid hole 803 is flushed on 1 wall of chromatographic column on the ball wall of part 801, and along 1 wall of chromatographic column flow down with
Silica Surface contacts, and is added after certain volume, opens force (forcing) pump 7 and opens simultaneously the cock 5 positioned at 1 bottom end of chromatographic column, carries out
Pressurization elution.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
Understanding without departing from the principles and spirit of the present invention can carry out these embodiments a variety of variations, modification, replace
And modification, the scope of the present invention is defined by the appended.
Claims (6)
1. it is a kind of it is novel can automatic feed liquor chromatographic apparatus, including chromatographic column (1), spherical geat container, speed-regulating type peristaltic pump (6)
And force (forcing) pump (7), which is characterized in that the spherical shape geat container includes spherical main body (3) and the ball affixed with spherical main body (3)
Shape filler tube (8), the spherical hopper pipe (8) include oval ball portion (801) and oval ball portion (801) upper end and spherical shape master
The cylindrical tube (802) that body (3) is connected, described ellipse ball portion (801) lower end closed, the ball wall of the ellipse ball portion (801)
On be provided with the identical fluid hole of multiple sizes (803), be connected with Y-type three way type connector (9) at the top of the spherical shape main body (3),
The speed-regulating type peristaltic pump (6) and the force (forcing) pump (7) are connected by pipeline with Y-type three way type connector (9) both ends respectively,
The both ends that the Y-type three way type connector (9) connect with the speed-regulating type peristaltic pump (6) and the force (forcing) pump (7) are equipped with valve
(10)；Spherical shape main body (3) bottom wall is abutted with the chromatographic column (1) upper end, cylindrical tube (802) upper end and the spherical shape
Main body (3) is connected to, and the chromatographic column (1) is inserted into spherical hopper pipe (8) lower end.
2. according to claim 1 it is a kind of it is novel can automatic feed liquor chromatographic apparatus, which is characterized in that the chromatographic column (1)
It is equipped between the spherical geat container and sleeve (2) is turned up, the height-regulating sleeve (2) passes through with the chromatographic column (1) upper end
It is threadedly coupled, spherical main body (3) bottom wall described at this time is abutted with height-regulating sleeve (2) upper surface, the spherical hopper pipe (8)
It is inserted into the chromatographic column (1) across the height-regulating sleeve (2).
3. according to claim 2 it is a kind of it is novel can automatic feed liquor chromatographic apparatus, which is characterized in that the height-regulating sleeve
(2) internal thread is offered, chromatographic column (1) upper end offers external screw thread with the part that sleeve (2) is connect is turned up.
4. according to claim 1 it is a kind of it is novel can automatic feed liquor chromatographic apparatus, which is characterized in that the fluid hole
(803) in multi-level distribution between oval ball portion (801) top and bottom, each layer of fluid hole (803) is all along ellipse
The circumferencial direction of ball portion (801) ball wall is uniformly distributed.
5. according to claim 1 it is a kind of it is novel can automatic feed liquor chromatographic apparatus, which is characterized in that the ellipse ball portion
(801) minimum distance of outer wall and chromatographic column (1) inner wall is two millimeters.
6. according to claim 1 it is a kind of it is novel can automatic feed liquor chromatographic apparatus, which is characterized in that the chromatographic column (1)
Lower port is provided with dismountable sand core (4), and sand core (4) lower end is provided with cock (5).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810783857.3A CN108744594A (en) | 2018-07-17 | 2018-07-17 | It is a kind of it is novel can automatic feed liquor chromatographic apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810783857.3A CN108744594A (en) | 2018-07-17 | 2018-07-17 | It is a kind of it is novel can automatic feed liquor chromatographic apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108744594A true CN108744594A (en) | 2018-11-06 |
Family
ID=63970400
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810783857.3A Withdrawn CN108744594A (en) | 2018-07-17 | 2018-07-17 | It is a kind of it is novel can automatic feed liquor chromatographic apparatus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108744594A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111921230A (en) * | 2020-08-22 | 2020-11-13 | 南京工业大学 | Chromatographic column auxiliary liquid adding device and liquid adding method |
-
2018
- 2018-07-17 CN CN201810783857.3A patent/CN108744594A/en not_active Withdrawn
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111921230A (en) * | 2020-08-22 | 2020-11-13 | 南京工业大学 | Chromatographic column auxiliary liquid adding device and liquid adding method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US20080178693A1 (en) | Valve | |
| CN108744595A (en) | A kind of chromatographic apparatus of automatic feed liquor | |
| CN108744594A (en) | It is a kind of it is novel can automatic feed liquor chromatographic apparatus | |
| CN207101968U (en) | A kind of chromatography column apparatus of automatic liquid-feeding | |
| CN108714320A (en) | It is a kind of can automatic feed liquor chromatographic apparatus | |
| CN108714319A (en) | A kind of automatic feed liquor chromatographic apparatus of combined type | |
| CN207153163U (en) | A kind of combined type column chromatography device | |
| CN108714318A (en) | A kind of automatic feed liquor chromatographic apparatus of sand core detachable type | |
| CN207600764U (en) | A kind of uniform leaching and can Stratified Sampling earth pillar device | |
| CN202136832U (en) | Normal pressure column chromatography device under closed environment | |
| CN203954734U (en) | The column chromatography combination unit that the adjustable column temperature of a kind of efficient dress post can freely break | |
| CN106290669A (en) | Liquid chromatographic system flowing is added and expanded in volume device mutually | |
| CN209204734U (en) | A kind of use for laboratory pressurizing chromatographic column | |
| CN211318328U (en) | Sample introduction chromatographic bottle for liquid chromatograph | |
| CN205169240U (en) | A proportioning device for distribution liquid dosage | |
| CN209155185U (en) | A kind of detachable multilayer chromatography chromatography column apparatus | |
| CN207755812U (en) | A kind of chromatographic column of constant pressure chromatography | |
| CN204107093U (en) | A kind of segmented cylinder chromatographic apparatus | |
| CN208626676U (en) | A kind of plus-minus pressure chromatographic column | |
| CN208927656U (en) | A kind of glass sample loading device for column chromatography | |
| CN206990516U (en) | It is a kind of can SPE headspace sampling device | |
| CN204439605U (en) | The superelevation loaded for narrow footpath chromatographic column press-fits post equipment | |
| CN204359761U (en) | high performance liquid chromatography ball | |
| CN206276066U (en) | Carry the chromatographic column of pressue device | |
| CN207708574U (en) | A kind of chromatographic column for the liquid feeding that pressurizes |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20181106 |