CN207755812U - A kind of chromatographic column of constant pressure chromatography - Google Patents
A kind of chromatographic column of constant pressure chromatography Download PDFInfo
- Publication number
- CN207755812U CN207755812U CN201721629779.9U CN201721629779U CN207755812U CN 207755812 U CN207755812 U CN 207755812U CN 201721629779 U CN201721629779 U CN 201721629779U CN 207755812 U CN207755812 U CN 207755812U
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- chromatographic column
- liquid
- cylinder
- chromatography
- glass
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Abstract
The utility model discloses a kind of chromatographic column of constant pressure chromatography, the top for chromatographing cylinder is equipped with chromatographic column sealing cover, the air inlet pipe for being inserted with the glass tube with breather valve on the chromatographic column sealing cover and being connected with gas pressure applicator；It chromatographs and is equipped with the liquid-feeding tube with liquid feeding cock on the right side of the epimere of cylinder, the liquid-feeding tube is communicated with the bottom of liquid storage device, the lower end of liquid-feeding tube, which is stretched into cylinder, is equipped with the filling opening that opening is gradually increased, and suspension ball is equipped in the filling opening, the suspension bulb diameter is more than the inside liquid feeding bore；Regulator is additionally provided on the chromatographic column sealing cover, the regulator includes the upper coarse and lower fine trapezoidal glass surge pipe being located on chromatographic column sealing cover, and trapezoidal step is equipped with glass slides pipe.The chromatographic column of the utility model constant pressure chromatography, pressure stability in holding post body ensure that eluent at the uniform velocity stablizes outflow, will not lead to the reduction of sample separating effect in chromatography process because of pressure change, efficiently solve the problems, such as that tradition chromatography post separation is slow-footed.
Description
Technical field
The utility model is related to the chromatographic columns that a kind of extraction separator more particularly to a kind of constant pressure chromatograph.
Background technology
Chromatographic column is a kind of common column chromatography separation technology, during the separation and Extraction of chemical product and natural products
It is widely used, but traditional chromatographic column separating rate is slow, experimental period is too long, causes the significant wastage of manpower and materials.
In the experiment for detaching compound using chromatographic column, need constantly to add eluant, eluent to chromatographic column upper end it is reserved be not filled by filler
Pillar top cavity in, due to the volume very little of cavity, generally require frequently to add.In addition, in chromatography process, it is
So that each component is preferably detached, the method that is often used eluent gradient elution, i.e., the eluent for first using polarity small into
Row elution, is gradually increased the content of the larger component of polarity in eluent.But the eluent for using polarity smaller, absorption-solution
The process of analysis is longer, and the time needed for column chromatography is also longer.Column chromatography is carried out by the way of pressurization, when being substantially shorter experiment
Between, but since chromatographic column internal pressure can not be excessive, it is desirable to it keeps stablizing, not so eluent flow rate is too fast or excessively slowly causes
Separating effect reduces, therefore needs in elution process to adjust pressure in time.
Utility model content
The technical problem to be solved by the utility model is to provide a kind of constant pressure chromatographies saved the time, improve separating effect
Chromatographic column.
To solve the above problems, the utility model provides a kind of chromatographic column of constant pressure chromatography, including liquid storage device, gas pressurized
Device, pressurization table, intake valve, regulator, air inlet pipe, chromatographic column sealing cover, breather valve, glass tube, liquid feeding cock, liquid-feeding tube,
Flow control valve, liquid discharging tube, flowmeter, filling opening, suspension ball, the chromatography cylinder for being divided into three sections of upper, middle and lower；Chromatograph the top of cylinder
End is equipped with chromatographic column sealing cover, be inserted with the glass tube with breather valve on the chromatographic column sealing cover and be connected with gas pressure applicator into
Tracheae；It chromatographs and is equipped with the liquid-feeding tube with liquid feeding cock on the right side of the epimere of cylinder, which communicates with the bottom of liquid storage device, liquid feeding
The lower end of pipe, which is stretched into cylinder, is equipped with the filling opening that opening is gradually increased, and suspension ball, the suspension ball are equipped in the filling opening
Diameter is more than the inside liquid feeding bore；The suspension ball support of eyelet is equipped on the outside of the filling opening, the suspension ball support is grid
Structure；The bottom end of chromatography cylinder is connected with the liquid discharging tube with flow control valve and flowmeter；
Regulator is additionally provided on the chromatographic column sealing cover, the regulator includes being located on chromatographic column sealing cover
Upper coarse and lower fine trapezoidal glass tube, trapezoidal step are equipped with glass slides pipe, and the glass slides pipe is equipped with bottom, bottom position
In on step, the glass slides pipe caliber is bigger than lower end glass tube, is slightly less than upper end glass tube caliber, the glass slides pipe
It can be slided up and down along upper end glass tube.
Preferably, water is housed in the glass slides pipe.
Preferably, the two sections of the chromatography cylinder and the stage casing of the chromatography cylinder are connected through a screw thread.
Preferably, the top of the glass surge pipe, which is equipped with, shrinks internal orifice, and the contraction internal orifice is equipped at tube wall
Flange, the aperture for shrinking internal orifice are less than the outer diameter of sliding glass pipe.
The utility model has the following advantages compared with prior art：
1, the stage casing that the two sections and chromatography cylinder of cylinder are chromatographed in the utility model is connected through a screw thread, and can be carried out
Dismounting, is easy to be cleaned, stored and transported, and any part damage can be replaced, and wear and tear expense is greatly reduced.
2, because liquid storage device is located on the right side of chromatography cylinder in the utility model, without removing chromatography column sealing when adding eluent
Lid, can continuously add eluent, convenient and efficient；Meanwhile, it is capable to which pressure, holding chromatographic column internal pressure are steady in adjustment column body in time
It is fixed, the silica gel tomography in chromatographic column is avoided, ensures that eluent at the uniform velocity stablizes outflow, saves the experimental implementation time, significantly improve sample
The separating effect of product.
3, gas pressure applicator can provide steady pressure for chromatographic column in the utility model, by adjusting intake valve, ventilation
Valve, can be with pressure in adjustable column body, by regulator, and pressure stability in holding post body ensures eluent at the uniform velocity stationary flow
Go out, the reduction of sample separating effect will not be caused in chromatography process because of pressure change, efficiently solves tradition chromatography post separation speed
Spend slow problem.
4, it is designed by the filling opening of original creation, liquid feeding liquid level stabilizing is kept, in stable height.
5, the utility model applies pressure automation, can effectively reduce the workload of people, easy to operate, easy to use,
Used component is commercial product, of low cost, is easily assembled to, and any part damage can be replaced, significantly
Reduce wear and tear expense.
Description of the drawings
Specific embodiment of the present utility model is described in further detail below in conjunction with the accompanying drawings.
Fig. 1 is the structural schematic diagram of the chromatographic column of the utility model constant pressure chromatography.
Fig. 2 is the upward view of the filling opening of the chromatographic column of the utility model constant pressure chromatography.
Fig. 3 is the structural schematic diagram of the regulator of the utility model.
Fig. 4 is the contraction internal orifice vertical view of the glass surge pipe of the utility model.
Specific implementation mode
As shown in Figs 1-4, the utility model discloses a kind of constant pressure chromatography chromatographic column, the chromatographic column include liquid storage device 7,
Gas pressure applicator 1, pressurization table 2, intake valve 3, regulator, air inlet pipe 4, chromatographic column sealing cover 5, breather valve 6, glass tube 16,
Liquid feeding cock 8, flow control valve 13, liquid discharging tube 12, flowmeter 14, filling opening 9, suspension ball 10, is divided into upper, middle and lower at liquid-feeding tube 17
Three sections of chromatography cylinder 11；The top for chromatographing cylinder 11 is equipped with chromatographic column sealing cover 5, and band logical is inserted on the chromatographic column sealing cover 5
The glass tube 16 of air valve 6 and the air inlet pipe 4 being connected with gas pressure applicator 5；It chromatographs and is equipped with band liquid feeding rotation on the right side of the epimere of cylinder 11
The liquid-feeding tube 17 of plug 2, which communicates with the bottom of liquid storage device 7, the lower end of liquid-feeding tube stretch into cylinder be equipped be open by
The filling opening 9 gradually increased, the filling opening 9 is interior to be equipped with suspension ball 10, and 9 diameter of the suspension ball is more than the inside liquid feeding bore；
9 outside of the filling opening is equipped with the suspension ball support 21 of eyelet, and the suspension ball support 21 is lattice structure；Chromatograph the bottom of cylinder 11
End is connected with the liquid discharging tube 12 with flow control valve 13 and flowmeter；
Regulator is additionally provided on the chromatographic column sealing cover 5, the regulator includes being located on chromatographic column sealing cover 5
Upper coarse and lower fine trapezoidal glass tube 18, trapezoidal step is equipped with glass slides pipe 19, and the glass slides pipe 19 sets
There are bottom, bottom to be located on step, 19 caliber of glass slides pipe is bigger than lower end glass tube, is slightly less than upper end glass tube caliber, institute
Stating glass slides pipe 19 can slide up and down along upper end glass tube.
Water is housed in the glass slides pipe 19.
The top of the glass surge pipe 18, which is equipped with, shrinks internal orifice 20, and the contraction internal orifice 20 is equipped with convex at close tube wall
Edge, the aperture for shrinking internal orifice are less than the outer diameter of sliding glass pipe 19.
Wherein：The two sections for chromatographing cylinder 11 are connect by screw thread 15 with the stage casing of chromatography cylinder 11.
Conduit is vacuum tube or rubber tube.
Gas pressure applicator 1 is air compressor or high pressure air bottle, can also use the device of other high pressure gas, rule
Lattice or size are determined by specific experiment situation, when chromatography cylinder 11 is smaller, use small gas pressurizer 1.
The pressure size that gas pressure applicator 1 applies into chromatography cylinder 11 is according to specific experiment situation and chromatographs cylinder 11
Depending on resistance to pressure, general warranty is more than standard atmospheric pressure, and eluent can be made to stablize and at the uniform velocity flowed out.
Liquid storage device 7 is replaceable, and capacity according to different can select.
When gas valve 6 is opened, the gas in release chromatography cylinder 11 reduces the air pressure inside chromatography cylinder 11；When
When gas valve 6 is closed, gas pressure applicator 1 injects air into chromatography cylinder 11, increases the air pressure inside chromatography cylinder 11.
The utility model use process is as follows：
(1) silica gel is stirred into homogenate：
Dry silica gel is added to solvent (the small solution of polarity is as solvent in eluent), is sufficiently stirred to be formed with glass bar
Homogenate.
(2) column is filled：11 column bottom of chromatography cylinder is stoppered with cotton, it is complete to open chromatography 11 lower section flow control valve 13 of cylinder, it will
Silica gel homogenate is poured into chromatography cylinder 11, makes its natural subsidence.
(3) loading crosses post separation：When silicagel column bed height stablizes constant, using dry or wet loading, after loading, add
Enter a small amount of eluent to cylinder certain altitude, chromatographic column of the connection equipped with gas pressure applicator 1, regulator and gas valve 6 is close
Capping 5, gas pressure applicator 1 are that chromatography cylinder 11 provides steady pressure, by pressure in the 2 observation column body of table that pressurizes, adjust ventilation
Pressure in cylinder is adjusted in valve 6；More than setting pressure value when, the glass slides pipe of the regulator pressure in cylinder
Float downward is pushed, with the increase of sliding tube down tube internal volume and a small amount of discharge of gas, pressure declines in cylinder, keeps cylinder
Interior pressure stability ensures that eluent at the uniform velocity stablizes outflow, by adjusting the amount of liquid in sliding tube, can set pressure in cylinder
Value.When chromatographing eluent deficiency in 11 body of cylinder, liquid feeding cock 8 is opened, eluent is added into cylinder, liquid feeding liquid level is high
When, suspension ball moves up, and filling opening is tapered inwards, arrives certain altitude, blocks liquid-feeding tube, stops liquid feeding；When liquid level declines, hang
Floating ball is fallen, and starts liquid feeding, repeats aforesaid operations, until isolated target substance.
Finally it should be noted that：The above descriptions are merely preferred embodiments of the present invention, is not limited to this
Utility model, although the utility model is described in detail with reference to the foregoing embodiments, for those skilled in the art
For, it still can be with technical scheme described in the above embodiments is modified, or to which part technical characteristic
Carry out equivalent replacement.Within the spirit and principle of the utility model, any modification, equivalent replacement, improvement and so on,
It should be included within the scope of protection of this utility model.
Claims (4)
1. a kind of chromatographic column of constant pressure chromatography, it is characterised in that：Including liquid storage device, gas pressure applicator, pressurization table, intake valve, tune
Pressure device, air inlet pipe, chromatographic column sealing cover, breather valve, glass tube, liquid feeding cock, liquid-feeding tube, flow control valve, liquid discharging tube, stream
Gauge, filling opening, suspension ball, the chromatography cylinder for being divided into three sections of upper, middle and lower；The top for chromatographing cylinder is equipped with chromatographic column sealing cover, should
The air inlet pipe for being inserted with the glass tube with breather valve on chromatographic column sealing cover and being connected with gas pressure applicator；The epimere for chromatographing cylinder is right
Side is equipped with the liquid-feeding tube with liquid feeding cock, which communicates with the bottom of liquid storage device, and the lower end of liquid-feeding tube, which is stretched into cylinder, to be set
There is the filling opening that opening is gradually increased, suspension ball is equipped in the filling opening, the suspension bulb diameter is more than in the liquid-feeding tube of the inside
Diameter, the filling opening outside are equipped with the suspension ball support of eyelet, and the suspension ball support is lattice structure；The bottom end for chromatographing cylinder connects
There is the liquid discharging tube with flow control valve and flowmeter；
Be additionally provided with regulator on the chromatographic column sealing cover, the regulator include be located at it is upper thick on chromatographic column sealing cover
Thin trapezoidal glass surge pipe down, trapezoidal step are equipped with glass slides pipe, and the glass slides pipe is equipped with bottom, bottom position
In on step, the glass slides pipe caliber is bigger than lower end glass tube, is slightly less than upper end glass tube caliber, the glass slides pipe
It can be slided up and down along upper end glass tube.
2. a kind of chromatographic column of constant pressure chromatography as described in claim 1, it is characterised in that：It is equipped in the glass slides pipe
Water.
3. a kind of chromatographic column of constant pressure chromatography as described in claim 1, it is characterised in that：The top of the glass surge pipe is set
There are contraction internal orifice, the contraction internal orifice being equipped with flange at tube wall, the aperture for shrinking internal orifice is less than sliding glass pipe
Outer diameter.
4. a kind of chromatographic column of constant pressure chromatography as described in claim 1, it is characterised in that：The two sections of the chromatography cylinder
It is connected through a screw thread with the stage casing of the chromatography cylinder.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721629779.9U CN207755812U (en) | 2017-11-29 | 2017-11-29 | A kind of chromatographic column of constant pressure chromatography |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721629779.9U CN207755812U (en) | 2017-11-29 | 2017-11-29 | A kind of chromatographic column of constant pressure chromatography |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207755812U true CN207755812U (en) | 2018-08-24 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721629779.9U Expired - Fee Related CN207755812U (en) | 2017-11-29 | 2017-11-29 | A kind of chromatographic column of constant pressure chromatography |
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| Country | Link |
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| CN (1) | CN207755812U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111921230A (en) * | 2020-08-22 | 2020-11-13 | 南京工业大学 | Chromatographic column auxiliary liquid adding device and liquid adding method |
-
2017
- 2017-11-29 CN CN201721629779.9U patent/CN207755812U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111921230A (en) * | 2020-08-22 | 2020-11-13 | 南京工业大学 | Chromatographic column auxiliary liquid adding device and liquid adding method |
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180824 Termination date: 20201129 |