CN204097485U - A kind of edible fungi liquid strain is cultivated and automatic vaccination device - Google Patents

A kind of edible fungi liquid strain is cultivated and automatic vaccination device Download PDF

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Publication number
CN204097485U
CN204097485U CN201420588153.8U CN201420588153U CN204097485U CN 204097485 U CN204097485 U CN 204097485U CN 201420588153 U CN201420588153 U CN 201420588153U CN 204097485 U CN204097485 U CN 204097485U
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vent fan
bacterium
valve
original seed
plunger
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刘彥超
刘国英
张宏田
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Abstract

A kind of edible fungi liquid strain is cultivated and automatic vaccination device, belongs to microbiology class culture device.This device comprise by fermentor tank tank body, Primary spawn hold concurrently device for culturing liquid strain that seeds depositing apparatus, inflation system and vent fan form with by the automatic vaccination device connecing bacterium device, polypropylene flow container brush, bacterium bag transporting mechanism and driving mechanism and form.This device adds thermal oil in interlayer, does temperature control medium with thermal oil, both can high-temperature sterilization, again can constant temperature culture.During sterilizing, thermal oil can meet the requirements of temperature at ambient pressure, not only safe but also easy and simple to handle; Connect bacterium device and can put into Jie Jun device sterilising chamber, strainer, valve and associated pipe are all in hot air sterilizer, and during nutrient solution sterilizing, they also simultaneously by sterilizing, have stopped the dead angle that miscellaneous bacteria is grown.Primary spawn is held concurrently seeds depositing apparatus, can cultivate original seed and may also be seeds depositing apparatus, closes and charges seed, and avoids polluting; Adopt automatic vaccination device in addition, production efficiency is high.

Description

A kind of edible fungi liquid strain is cultivated and automatic vaccination device
Technical field
The utility model belongs to microbiology class culture device.Specifically a kind of edible fungi liquid strain is cultivated and automatic vaccination device.
Background technology
The production of edible fungi liquid strain, more universal at present.But edible fungus liquid production equipment known at present can only in the autumn, in the winter, season in spring three produces.Summer high temperature wets more, seriously polluted, has to stop production.And current production equipment can not ensure absolutely success, although pollution rate is lower, if carry out scale operation, its loss also can not be born.Crucial problem is, existing device definitely can not stop the intrusion of miscellaneous bacteria, pollute in operation and operational process.Control the prerequisite of miscellaneous bacteria at fungistat under, in the spring, in the winter, the autumn produces the yield rate that can only ensure 95%, in summer, because pollution rate is high, has to stop work during the dog days.Current, be the critical period of Agricultural Transformation, agriculture production is being rapidly to large-scale, mass-producing future development.Edible Fungi is listed in by country the project of giving special assistance to, peasant in all parts of the country, and very high to the enthusiasm of development Edible Fungi, various edible mushroom, the market demand of auricularia auriculajudae is also very large.Urgent need can whole year production, particularly production in summer also non-staining edible fungi liquid strain production unit.In order to realize whole year production, large-scale production, have studied this covering device.
Summary of the invention
The utility model provides a kind of edible fungi liquid strain and cultivates and automatic vaccination device.Emphasis solves a living contaminants difficult problem and automatically connects bacterium.Long-term production can be realized, correct in operation, under the prerequisite that original seed is pure, guarantee not contaminated.
The technical solution adopted in the utility model is: a kind of edible fungi liquid strain is cultivated and automatic vaccination device, it comprises fermentor tank tank body and Primary spawn and to hold concurrently seeds depositing apparatus, it also comprises inflation system, the first vent fan, the second vent fan, connect bacterium device, polypropylene flow container brush, bacterium bag transporting mechanism and driving mechanism, the top of described fermentor tank tank body is provided with venting hole and throws original seed mouth and to hold concurrently thief hole, venting port is connected with the first vent fan, throws original seed mouth thief hole of holding concurrently and is connected with the throwing original seed pipe of outside through throwing original seed sampling valve of holding concurrently, the bottom of described fermentor tank tank body is provided with discharge gate, trumpet type air head is housed above discharge gate, and the interchanger of the suitable for reading and inflation system of trumpet type air head is connected, and has gap at the bottom of end opening and tank, a hollow float is set in trumpet type air head, at discharge gate, blow-off valve is housed, described fermentor tank tank body is provided with temperature sensor, the middle and lower part of fermentor tank tank body periphery is provided with oil bath interlayer, Jie Jun device sterilising chamber and Jie Jun device sterilising chamber sealing cover, be provided with dry sterilization room on the top of oil bath interlayer, be provided with the first strainer, the second strainer, the 3rd strainer, the first vent fan and the second vent fan in dry sterilization indoor, adding thermal oil in described oil bath interlayer is heat-conducting medium, and be provided with electric heating tube and interchanger in described oil bath interlayer, oil bath interlayer pipeline is connected with air-cooled radiator, described inflation system Bian air pump connects relief valve, under meter, tensimeter and airheater successively, airheater is by being connected interchanger after the first strainers and second strainer of two series connection, two-way is divided in the outlet of interchanger, one tunnel connects trumpet type air head through culture tank intake valve, and another road is connected Primary spawn through original seed intake valve and holds concurrently seeds depositing apparatus after the 3rd strainer, the described bacterium device that connects comprises plunger and plunger body, silica-gel plate is pressed with after plunger body, plunger is inserted at the center of silica-gel plate, the side of silica-gel plate is plunger, opposite side is guide bar, spring is equipped with in the guide bar side of silica-gel plate, spring handle plunger pushes the bottom of plunger body to, seal the import of bacterium liquid, plunger body upper front end dress needle tubing, in have column reverse checkvalve to be pressed in the chamber that needle tubing is connected with plunger body outlet that bacterium liquid exports, the column reverse checkvalve the other end withstands on elastic weld wood, being contact outside elastic weld wood, is the import of bacterium liquid below plunger body, connect on bacterium device side frame and polypropylene flow container brush is housed, described bacterium bag transporting mechanism adopts the travelling belt being provided with travelling belt passive tooth, described driving mechanism adopts motor to drive transmission shaft, transmission shaft is provided with an individual gears matched with travelling belt passive tooth and a ditch sheave, the one end of the shifting fork bar supported by declutch shift shaft is stuck in the chute of ditch sheave, the prong of the other end be stuck in be positioned at plunger body connect in bacterium device reversed column, the anchor ring of described ditch sheave is provided with end to end and that connect the action of bacterium device and match the first skewed slot A successively, second skewed slot B, first straight trough C, 3rd skewed slot D and the second straight trough E, match in the position that one end of action and shifting fork bar that described individual gears stirs travelling belt passive tooth is stuck in ditch sheave first skewed slot A and the second straight trough E.
Described Primary spawn seeds depositing apparatus of holding concurrently adopts an O-ring barrel shaped structure, drum center is provided with an inverted L-shaped pipe, the lower end of the vertical component of inverted L-shaped pipe is close at the bottom of bucket, and transverse tube stretches out outside bucket at 5mm place, bucket top, is provided with and drops into female kind, exports original seed and be vented three and use mouth at the opposite side of transverse tube.
Described first vent fan and the second vent fan all adopt one to seal oval bucket, median septum is divided into vent fan F side and vent fan G side the oval bucket of sealing, a hole is respectively opened above median septum both sides, pipe is inserted in the hole of median septum both sides, the lower nozzle of pipe is close at the bottom of bucket, upper orifice flushes with bung, wherein be provided with round platform in the pipe of vent fan F side, put into the ball forming reverse checkvalve, this holes is detained sealing pan shape lid, inlet mouth is provided with in addition at bucket top, vent fan F side, safety valve is provided with at vent fan G side roof part, vent fan valve (33) and tensimeter, vent fan G side bottom is provided with tapping valve.
Described Jie Jun device sterilising chamber sealing cover, be made up of two pot shape cover buckles, the above is silica gel pot together, and here is porose white steel pot.
The beneficial effects of the utility model are: this edible fungi liquid strain is cultivated to comprise with automatic vaccination device and to be held concurrently device for culturing liquid strain that seeds depositing apparatus, inflation system and vent fan form and by the automatic vaccination device connecing bacterium device, polypropylene flow container brush, bacterium bag transporting mechanism and driving mechanism and form by fermentor tank tank body, Primary spawn.This device adds thermal oil in interlayer, does temperature control medium with thermal oil, both can high-temperature sterilization, again can constant temperature culture.During sterilizing, thermal oil can meet the requirements of temperature at ambient pressure, not only safe but also easy and simple to handle; Heating tube moves in thermal oil, and relieve because heating tube is in tank, nutrient solution is charred the disadvantage be easily bonded on heating tube, and culture tank is also easy to cleaning; Arrange Jie Jun device sterilising chamber, blow-off valve is fixed on indoor, then connecing bacterium device and pipeline puts into Jie Jun device sterilising chamber; Strainer, valve and associated pipe are all indoor dry sterilization, and during nutrient solution sterilizing, they also simultaneously by sterilizing, have stopped the dead angle that miscellaneous bacteria is grown.Adopt automatic vaccination device in addition, production efficiency is high.
Accompanying drawing explanation
Fig. 1 is that a kind of edible fungi liquid strain cultivates the structural representation with automatic vaccination device.
Fig. 2 is inflation system schematic diagram.
Fig. 3 is the structural representation of vent fan.
Fig. 4 is inverted Primary spawn when throwing original seed to hold concurrently seeds depositing apparatus schematic diagram.
Fig. 5 connects bacterium device sterilizing schematic diagram.
Fig. 6 connects bacterium device structure iron.
Fig. 7 is the structure front view of automatic Inoculation machine.
Fig. 8 is the structure side view of automatic Inoculation machine.
Fig. 9 is ditch sheave surface stretch-out view.
Figure 10 is that Inoculation machine bacterium bag moves, inserting needle, discharge of bacteria liquid, withdraw of the needle schematic diagram.
Figure 11 is that when cultivating original seed, Primary spawn seeds depositing apparatus of holding concurrently connects vent fan schematic diagram.
In figure: 1, sterilizing is held concurrently proving room, and 2, oil bath interlayer, 3, venting port, 4, throw original seed mouth to hold concurrently thief hole, 5, safety valve, 6, discharge gate, 7, temperature sensor, 8, upper cover, 9, electric heating tube, 10, air-cooled radiator, 11, blow-off valve, 12, Jie Jun device sterilising chamber, 13, the sealing cover of Jie Jun device sterilising chamber, 14, interchanger, 15, dry sterilization room, 16, lagging casing, 17, airheater, 18, under meter, 19, relief valve, 20, air pump, 21, trumpet type air head, 22, Primary spawn is held concurrently seeds depositing apparatus, and 23, original seed gas-filled valve, 23a, first flexible pipe, 24, throw original seed pipe, 24a, second flexible pipe, 25, drop into female kind, export original seed and exhaust three mouth, 26a, first strainer, 26b, second strainer, 26c, 3rd strainer, 27, ball float, 28a, first vent fan, 28b, second vent fan, 29, air temperature sensor, 30, inverted L-shaped pipe, 31, throw original seed to hold concurrently sampling valve, 32, culture tank intake valve, 33, vent fan valve, 34, original seed intake valve, 35, tensimeter, 36, tapping valve, 37, tensimeter, 38, condensation trap, 39, connect bacterium device, 40, carrier pipe, 41, guide bar, 42, elastic weld wood, 43, needle tubing, 44, catch, 45, plunger, 46, bacterium liquid exports, and 47, column reverse checkvalve, 48, the import of bacterium liquid, 49, spring capping, 50, plunger body, 51, silica-gel plate, 52, contact, 53, stage clip, 54, connect bacterium device reversed column, 55, guide wire, 56, shifting fork bar, 57, declutch shift shaft, 58, transmission shaft, 59, ditch sheave, 60, individual gears, 61, travelling belt, 62, motor, 63, travelling belt passive tooth, 64, polypropylene flow container brush, 65, bacterium bag, 66 inlet mouths.
Embodiment
Structure and the use of this equipment is introduced further below in conjunction with accompanying drawing.
Fig. 1,2,3,6,7,8 shows a kind of edible fungi liquid strain and cultivates the structural representation with automatic vaccination device.Edible fungi liquid strain is cultivated with automatic vaccination device comprises fermentor tank tank body, Primary spawn holds concurrently seeds depositing apparatus 22, inflation system, the first vent fan 28a, the second vent fan 28b, connect bacterium device 39, polypropylene flow container brush 64, bacterium bag transporting mechanism and driving mechanism.
The top that Fig. 1 shows fermentor tank tank body is provided with venting hole 3 and throws original seed mouth and to hold concurrently thief hole 4, and venting port 3 is connected with the first vent fan 28a, and throwing original seed mouth thief hole 4 of holding concurrently is connected with the throwing original seed pipe 24 of outside through throwing original seed sampling valve 31 of holding concurrently.The bottom of fermentor tank tank body is provided with discharge gate 6, trumpet type air head 21 is housed above discharge gate 6, the interchanger 14 of the suitable for reading and inflation system of trumpet type air head 21 is connected, gap is had at the bottom of end opening and tank, a hollow float 27 is set in trumpet type air head 21, at discharge gate 6, blow-off valve 11 is housed.Fermentor tank tank body is provided with temperature sensor 7, the middle and lower part of fermentor tank tank body periphery is provided with oil bath interlayer 2 and Jie Jun device sterilising chamber 12, be provided with dry sterilization room 15 on the top of oil bath interlayer 2, in dry sterilization room 15, be provided with the first strainer 26a, the second strainer 26b, the 3rd strainer 26c, the first vent fan 28a and the second vent fan 28b.Be provided with electric heating tube 9 and interchanger 14 in oil bath interlayer 2, oil bath interlayer 2 adopts pipeline to be connected with air-cooled radiator 10.
Illustrate in Fig. 2 that Primary spawn seeds depositing apparatus 22 of holding concurrently adopts an O-ring barrel shaped structure, drum center is provided with an inverted L-shaped pipe 30, the lower end of the vertical component of inverted L-shaped pipe 30 is close at the bottom of bucket, transverse tube stretches out outside bucket at 5mm place, bucket top, is provided with and drops into female kind, exports original seed and exhaust three mouth 25 at the opposite side of transverse tube.
During Fig. 3 shows, the first vent fan 28a(comprises the second vent fan 28b) adopt one to seal oval bucket, median septum is divided into vent fan F side and vent fan G side the oval bucket of sealing, a hole is respectively opened above median septum both sides, pipe is inserted in the hole of median septum both sides, the lower nozzle of pipe is close at the bottom of bucket, upper orifice flushes with bung, wherein be provided with round platform in the pipe of vent fan F side one, put into the ball forming reverse checkvalve, this holes is detained sealing pan shape lid, safety valve 5 is provided with in addition at bucket top, vent fan G side, tensimeter 35, vent fan valve 33, in vent fan G side, bottom of the barrel is provided with tapping valve 36, vent fan F side is provided with inlet mouth 66.
Illustrate in Fig. 1,2 that inflation system Bian air pump 20 connects relief valve 19, under meter 18, tensimeter 37 and airheater 17 successively, airheater 17 is by being connected interchanger 14 after the first strainer 26a and the second strainer 26b of two series connection, two-way is divided in the outlet of interchanger 14, one tunnel connects trumpet type air head 21 through culture tank intake valve 32, and another road is connected Primary spawn through original seed intake valve 34 and holds concurrently seeds depositing apparatus 22 after the 3rd strainer 26c.
Fig. 6 shows and connects bacterium device and comprise plunger 45 and plunger body 50, and plunger body 50 is pressed with silica-gel plate 51 below, and plunger 45 is inserted at the center of silica-gel plate 51, the side of silica-gel plate 51 is plungers 45, opposite side is guide bar 41, is welded with disk between plunger 45 and guide bar 41, and fixing silica-gel plate 51 also seals.Spring 53 is equipped with in guide bar 41 side of silica-gel plate 51, spring 53 pushes the bottom of plunger body 50 to plunger 45, seal bacterium liquid import 48, plunger body 50 upper front end dress needle tubing 43, having column reverse checkvalve 47 to be pressed in bacterium liquid in the chamber that needle tubing 43 is connected with plunger body 50 exports in the outlet of 46, the other end of column reverse checkvalve 47 withstands on elastic weld wood 42, has contact 52 outside elastic weld wood 42, is bacterium liquid import 48 below plunger body 50; Connect on bacterium device 39 side frame and polypropylene flow container brush 64 is housed.
Fig. 7,8 shows bacterium bag transporting mechanism and adopts the travelling belt 61 being provided with travelling belt passive tooth 63.Driving mechanism adopts motor 62 to drive transmission shaft 58, transmission shaft 58 is provided with an individual gears matched with travelling belt passive tooth 63 60 and a ditch sheave 59, the one end of the shifting fork bar 56 supported by declutch shift shaft 57 is stuck in the chute of ditch sheave 59, the prong of the other end be stuck in be positioned at plunger body 50 connect in bacterium device reversed column 54, the anchor ring of described ditch sheave 59 is provided with end to end and that connect bacterium device 39 action and match the first skewed slot A successively, second skewed slot B, first straight trough C, 3rd skewed slot D and the second straight trough E, the action that described individual gears 60 stirs travelling belt passive tooth 63 matches with the position that one end of shifting fork bar 56 is stuck in ditch sheave 59 first skewed slot A and the second straight trough E.
Adopt technique scheme, first inject nutrient solution.Close the lid 8, and seal.Close and throw original seed mouth valve 31, fermentor tank intake valve 32, vent fan valve 33, tapping valve 36, condensation trap 38.Open original seed intake valve 34, defeated original seed pipe 24 is connected with original seed gas-filled valve 23, and pipe is attached on tank skin.The carrier pipe 40 connecing bacterium device 39 is connected with the interface of blow-off valve 11, after press seal, bacterium device 39 will be connect take off from guide wire 55, put into Jie Jun device sterilising chamber 12 together with carrier pipe 40, cover the sealing cover 13 (as shown in Figure 5) of Jie Jun device sterilising chamber.After intensification, air expansion in sterilising chamber, silica gel lid presses to porose white steel lid, recovers former state, gets final product safety anti-explosive, can ensure again the sterile state of room air after cooling.To electric heating tube 9 power transmission, the thermal oil in oil bath interlayer 2 heats up.After opening vent fan valve 33 exhausting cold air by regulation, valve-off.Sterilizing hold concurrently proving room 1 temperature sensor socket 7 in be inserted with temperature sensor, when temperature reaches 122 DEG C of setting, temperature controller can control the sterilizing of electric heating tube 9 pairs of nutrient solution constant temperature.Be placed in three strainers in dry sterilization room 15, the first vent fan 28a and the second vent fan 28b, the vapor pipe of venting port 3 to the first vent fan 28a pastes tank skin simultaneously, is attached to defeated original seed pipe 24 on tank skin and original seed gas-filled valve 23 can obtain thorough sterilizing.Blow-off valve 11 in interchanger 14 in preheating interlayer 2 in thermal oil and Jie Jun device sterilising chamber 12, connecing bacterium device 39, carrier pipe 40 also can thoroughly sterilizing.
After completing sterilizing, open condensation trap 38 and drive scatterer 14, lowering the temperature to culture tank.Open vent fan valve 33 discharging hot air, when culture tank pressure drop is to 0.05MPa, airheater 17 electrified regulation, by air temperature sensor 29 by homo(io)thermism at about 200 DEG C.When temperature reaches 200 DEG C, booster air pump 20 is inflated, and opens culture tank intake valve 32.Mix up relief valve 19 and under meter 18, tensimeter 37 shows force value, and when inflation pressure is higher than tank internal pressure, air is filled with in tank.All the time all keep malleation in culture tank, invade to prevent miscellaneous bacteria.Warm air enters and is fixed on the first strainer 26a in dry sterilization room 15 and the second strainer 26b, while warm air passes through, also be heated more than 100 DEG C, the miscellaneous bacteria can be detained not killing in airheater 17 is in the filter scalded dead, ensures that the air entered in tank is pure.Air moves on, and enters interchanger 14, regulates air themperature.Heat exchanger exit place, is divided into two outlets, and original seed intake valve 34 is installed in an outlet, connects with original seed gas-filled valve 23 through the 3rd strainer 26c.Another outlet installs culture tank intake valve 32 more by the road by after trumpet type air head 21 disturbance nutrient solution and oxygen supply, discharges, by vent fan valve 33 regulating tank internal pressure through the first vent fan 28A.
Fig. 4 shows Primary spawn seeds depositing apparatus of holding concurrently and throws original seed schematic diagram.Figure 11 shows Primary spawn schematic diagram.Aseptically, the mouth of pipe of inverted L-shaped pipe 30 of seeds depositing apparatus 22 of being held concurrently by the Primary spawn filling nutrient solution connects original seed gas-filled valve 23, open original seed intake valve 34, to hold concurrently inflation in seeds depositing apparatus 22 to Primary spawn, plant from input is female, export original seed and be vented three with after the female kind of mouth of pipe input of mouth 25, connecting with the same second vent fan 28b of pipe, cultivate original seed.After Primary spawn is good, when culture-liquid temp in tank drops to suitable growth temperature, aseptically, at input original seed, female to plant from input, export original seed and exhaust three takes off with mouth 25 pipe connecting be connected with the second vent fan 28b, plug and throw original seed pipe 24, open throwing original seed and to hold concurrently sampling valve 31, Primary spawn seeds depositing apparatus 22 of holding concurrently is inverted, cultured original seed can be dropped in culture tank (as shown in Figure 4).During strain cultivation, carry out purity detecting.Open and throw original seed and to hold concurrently sampling valve 31, owing to being malleation in tank, liquid spawn can be flowed out from throwing original seed pipe 24 mouth of pipe, proposes, i.e. sampling throwing original seed pipe 24 mouth of pipe from disinfecting liquid.If insufficient pressure, the aperture of the vent fan valve 33 on adjustable first vent fan 28a, until bacterial classification is discharged.When the bacterium ball of cultivated liquid spawn reaches requirement density, namely available automatic Inoculation machine connects bacterium.
Fig. 6,7,8,9,10 shows structure and the working process of automatic Inoculation machine.In Fig. 7,8, methods, rack is fixed wtih guide wire 55 in travelling belt 61 side, the guide bar 41 connect on bacterium device 39 puts into guide wire 55, connects bacterium device 39 and reciprocally moves.Connect on bacterium device 39 side frame and polypropylene flow container brush 64 is housed.Transmission shaft 58 is fixed two wheels, ditch sheave 59 and individual gears 60.Individual gears 60 only has a tooth, moves with 1/4th circle ejection travelling belts, and all the other 3/4ths circle idle running, during idle running, travelling belt does not move.Ditch sheave 59 circumference outerplanar has groove, and groove divides 4 sections, and every section accounts for 1/4th of wheel face girth, wherein two sections parallel with wheel, be distributed in wheel face both sides and opposing, another two sections is skewed slot, end to end with parallel-groove, on the contrary oblique.In fig .9, ditch sheave 59 rotates, and shifting fork bar 56 lower end enters A, and during the oblique section of B, D, shifting fork bar 56 promotes to connect bacterium device 39 and moves, and completes and thrusts, discharge of bacteria liquid, exit three actions, and when entering C section, needle tubing 43 suspends for a moment to discharge remaining liquid in bacterium bag.At this moment the tooth of individual gears 60 is interval in 3/4ths circle idle running, bacterium bag 65 transfixion.The tooth top of individual gears 60 pushes away travelling belt 61 when moving, and shifting fork bar 56 lower end is positioned at the E section of groove wheel 59, and when bacterium bag 65 moves, connect bacterium device 39 transfixion, needle tubing exit status, does not interfere with each other.
When preparing to connect bacterium, unloading the sealing cover 13 of Jie Jun device sterilising chamber, taking out and connecing bacterium device 39, opening carrier pipe 40, connect what connect bacterium device 39 the groove that bacterium device reversed column 54 puts into shifting fork bar 56 upper end, guide bar 41 puts into guide wire 55, fastens guide wire 55, and fixing.Guide bar 41 can to-and-fro movement in guide wire 55.Shifting fork bar 56 lower end is inserted in the groove of ditch sheave 59.Outward winding blow-off valve 11, start Inoculation machine, ditch sheave 59 rotates, and promote shifting fork bar 56 with declutch shift shaft 57 for fulcrum, order connects bacterium device 39 to-and-fro movement.The plunger body 50 and the plunger 45 that make to connect bacterium device 39 do relative motion, and suction bacterium liquid, when needle tubing 43 discharges bacterium liquid, shuts down.Put bacterium bag 65 on a moving belt, load onto polypropylene flow container brush 64, can start shooting and connect bacterium.
After start, when individual gears 60 to be touched in travelling belt passive tooth 63(Fig. 9,10 shown in A), promote travelling belt 61 and move, bacterium bag is also and then mobile.When individual gears 60 rotates 80 degree of angles, the monodentate of individual gears 60 departs from travelling belt passive tooth 63, and travelling belt 61 stops mobile, before bacterium bag is just parked in needle tubing 43.During this, shifting fork bar 56 lower end is in the L side (shown in Fig. 9) in groove wheel 59, and the E section parallel with ditch sheave 59, connects bacterium device and there be not action, is in bacterium state waiting.
Ditch sheave 59 is rotated further, and bacterium bag 65 is as you were.Shifting fork bar 56 lower end enters the oblique section A in ditch sheave 59, promotes to connect bacterium device inserting needle, thrusts (in Fig. 9,10 shown in C) in bacterium bag 65.During close to oblique section A end, the catch 44 of guide bar end is guided pipe 55 to be stopped, plunger 45 can not move, but shifting fork bar 56 also continues to push away plunger body 50 to be moved, overcome spring pressure, plunger 45 and plunger body 50 start relative movement, and plunger 45 retreats in plunger body 50, expose bacterium liquid import 48, bacterium liquid is sucked in plunger body 50.Ditch sheave 59 is rotated further, shifting fork bar 56 lower end enters also very short B section contrary to A section direction (shown in Fig. 9), plunger body 50 back moves, under the action of the spring, plunger 45 is pushed to bottom plunger body 50, seal again bacterium liquid import 48, back down column reverse checkvalve 47 simultaneously and bacterium liquid is exported 46 through bacterium liquid, injected in bacterium bag by needle tubing 43.When having bacterium liquid, column reverse checkvalve 47 is pushed, and reacts on silicon elastic weld wood 42 and promotes contact 52 and open, after discharge opeing, under elastic weld wood 42 suppresses, column reverse checkvalve 47 sets back, and contact 52 closes, if line clogging, do not suck bacterium liquid in plunger body 50, column reverse checkvalve 47 can not push, and contact 52 does not just have action, control Inoculation machine running and report to the police, ensureing that every bag can tap into bacterial classification.
Ditch sheave 59 is rotated further, and shifting fork bar 56 lower end enters the C section parallel with ditch sheave, and shifting fork bar 56 does not move, and needle tubing 43 is parked in bacterium bag, continues discharge of bacteria liquid (in Fig. 9,10 shown in D).After shifting fork bar 56 lower end enters the oblique section of D, needle tubing 43 shifts out bacterium bag 65.During inserting needle to needle tubing 43 shifts out bacterium bag 65, bacterium bag 65 does not move.After needle tubing 43 shifts out bacterium bag 65, individual gears 60 touches again travelling belt passive tooth 63, and bacterium bag 65 moves, and next bag connects bacterium to start.
When moving, connect bacterium bag acupuncture mouth and be fixed in smearing containing the brush of polypropylene flow container brush 64 of this side, hydrothermal solution cools, and bond with bag film, acupuncture mouth is closed, and connects bacterium and completes again.Brush is contained in the bottom that polypropylene tank is housed, and has heating unit in tank, polypropylene can be liquefied.
Said apparatus has following features:
1, in interlayer, thermal oil is added, need not Sealed sandwich.Temperature control medium is done with thermal oil, both can high-temperature sterilization, again can constant temperature culture.During sterilizing, thermal oil can meet the requirements of temperature at ambient pressure.Not only safe but also easy and simple to handle;
2, decrease the outlet of tank body, three outlets that full tank is only necessary, and each external Link Port takes tight closed measure, decrease miscellaneous bacteria and enter passage in tank;
3, safety valve and tensimeter are arranged on vent fan, stop miscellaneous bacteria and enter;
4, heating tube moves in thermal oil, and relieve because heating tube is in tank, nutrient solution is charred the disadvantage be easily bonded on heating tube, and culture tank is also easy to cleaning;
5, the favourable insulation of additional thermal insulation layer, save energy;
6, pin type blow-off valve has the effect of Open Top Can bottom sediment, and inflation outlet, facing to discharge gate, can blow away throw out, prevent discharge from blocking;
7, arrange valve and Jie Jun device sterilising chamber, valve, will connect bacterium device and pipeline puts into indoor within it, while nutrient solution sterilizing, close sterilizing, will take out during application, guarantee in system without miscellaneous bacteria;
8, strainer, valve and associated pipe are all in hot air sterilizer, and during nutrient solution sterilizing, they also simultaneously by sterilizing, have stopped the dead angle that miscellaneous bacteria is grown; Need not otherwise sterilized being taken off, both save trouble, additionally reducing because loading and unloading the chance causing miscellaneous bacteria to invade;
9, when nutrient solution sterilizing, the interchanger in interlayer, simultaneously by sterilizing, when constant temperature culture, while reducing the air themperature entered, also raises the temperature of thermal oil;
10, Primary spawn seeds depositing apparatus of holding concurrently not only can have been cultivated original seed but also can connect and throws original seed pipe cultured original seed is directly dropped into culture tank, closedly charged seed, and avoided polluting;
11, Primary spawn is held concurrently seeds depositing apparatus outlet, flatly three use, and when not taking over road, be throw femalely to plant mouth, connecing vent fan is venting port, connects to throw original seed pipe and be inverted by Primary spawn device to turn and original seed can be compressed into culture tank;
12, will enter the air heating of tank, and reach 200 DEG C, after entering strainer in well heater, strainer also can be warmed to more than 100 DEG C by high temperature air, can be all boiling hot dead by the miscellaneous bacteria flowing through well heater and be trapped in strainer, ensures that air inlet is without miscellaneous bacteria;
13, liquid-spawn inoculation automatization, decreasing pollution chance, saves artificial;
14, connect between bacterium device plunger body and the insert port of plunger and seal with silica-gel plate, do not affect it by force to movement, also avoid miscellaneous bacteria to enter;
15, special valve, ensures that miscellaneous bacteria can not be entered by this.

Claims (4)

1. an edible fungi liquid strain is cultivated and automatic vaccination device, it comprises fermentor tank tank body and Primary spawn and to hold concurrently seeds depositing apparatus (22), it is characterized in that: it also comprises inflation system, first vent fan (28a), second vent fan (28b), connect bacterium device (39), polypropylene flow container brush (64), bacterium bag transporting mechanism and driving mechanism, the top of described fermentor tank tank body is provided with venting hole (3) and throws original seed mouth and to hold concurrently thief hole (4), venting port (3) is connected with the first vent fan (28a), throw original seed mouth thief hole (4) of holding concurrently to be connected with the throwing original seed pipe (24) of outside through throwing original seed sampling valve (31) of holding concurrently, the bottom of described fermentor tank tank body is provided with discharge gate (6), in discharge gate (6) top, trumpet type air head (21) is housed, the interchanger (14) of the suitable for reading and inflation system of trumpet type air head (21) is connected, gap is had at the bottom of end opening and tank, a hollow float (27) is set in trumpet type air head (21), at discharge gate (6), blow-off valve (11) is housed, described fermentor tank tank body is provided with temperature sensor (7), the middle and lower part of fermentor tank tank body periphery is provided with oil bath interlayer (2), Jie Jun device sterilising chamber (12) and Jie Jun device sterilising chamber sealing cover (13), be provided with dry sterilization room (15) on the top of oil bath interlayer (2), in dry sterilization room (15), be provided with the first strainer (26a), the second strainer (26b), the 3rd strainer (26c), the first vent fan (28a) and the second vent fan (28b), adding thermal oil in described oil bath interlayer (2) is heat-conducting medium, be provided with electric heating tube (9) and interchanger (14) in described oil bath interlayer (2), oil bath interlayer (2) pipeline is connected with air-cooled radiator (10), described inflation system Bian air pump (20) connects relief valve (19), under meter (18), tensimeter (37) and airheater (17) successively, airheater (17) is connected interchanger (14) by first strainer (26a) of two series connection afterwards with the second strainer (26b), two-way is divided in the outlet of interchanger (14), one tunnel connects trumpet type air head (21) through culture tank intake valve (32), and another road is connected Primary spawn through original seed intake valve (34) and holds concurrently seeds depositing apparatus (22) after the 3rd strainer (26c), the described bacterium device that connects comprises plunger (45) and plunger body (50), plunger body (50) is pressed with silica-gel plate (51) below, plunger (45) is inserted at the center of silica-gel plate (51), the side of silica-gel plate (51) is plunger (45), opposite side is guide bar (41), spring (53) is equipped with in guide bar (41) side of silica-gel plate (51), spring (53) pushes the bottom of plunger body (50) to plunger (45), seal bacterium liquid import (48), plunger body (50) upper front end dress needle tubing (43), having column reverse checkvalve (47) to be pressed in bacterium liquid in the chamber that needle tubing (43) is connected with plunger body (50) exports in the outlet of (46), column reverse checkvalve (47) the other end withstands on elastic weld wood (42), elastic weld wood (42) outside is contact (52), it is bacterium liquid import (48) below plunger body (50), connect on bacterium device (39) side frame and polypropylene flow container brush (64) is housed, described bacterium bag transporting mechanism adopts the travelling belt (61) being provided with travelling belt passive tooth (63), described driving mechanism adopts motor (62) to drive transmission shaft (58), transmission shaft (58) is provided with an individual gears (60) matched with travelling belt passive tooth (63) and a ditch sheave (59), the one end of the shifting fork bar (56) supported by declutch shift shaft (57) is stuck in the chute of ditch sheave (59), the prong of the other end be stuck in be positioned at plunger body (50) connect in bacterium device reversed column (54), the anchor ring of described ditch sheave (59) is provided with end to end and that connect bacterium device (39) action and match the first skewed slot A successively, second skewed slot B, first straight trough C, 3rd skewed slot D and the second straight trough E, the action that described individual gears (60) stirs travelling belt passive tooth (63) matches with the position that one end of shifting fork bar (56) is stuck in ditch sheave (59) first skewed slot A and the second straight trough E.
2. a kind of edible fungi liquid strain according to claim 1 is cultivated and automatic vaccination device, it is characterized in that: described Primary spawn hold concurrently seeds depositing apparatus (22) adopt an O-ring barrel shaped structure, drum center is provided with an inverted L-shaped pipe (30), the lower end of the vertical component of inverted L-shaped pipe (30) is close at the bottom of bucket, transverse tube stretches out outside bucket at 5mm place, bucket top, is provided with and drops into female kind, exports original seed and exhaust three mouth (25) at the opposite side of transverse tube.
3. a kind of edible fungi liquid strain according to claim 1 is cultivated and automatic vaccination device, it is characterized in that: described first vent fan (28a) and the second vent fan (28b) all adopt one to seal oval bucket, median septum is divided into vent fan F side and vent fan G side the oval bucket of sealing, a hole is respectively opened above median septum both sides, pipe is inserted in the hole of median septum both sides, the lower nozzle of pipe is close at the bottom of bucket, upper orifice flushes with bung, wherein be provided with round platform in the pipe of vent fan F side, put into the ball forming reverse checkvalve, this holes is detained sealing pan shape lid, inlet mouth (66) is provided with in addition at bucket top, vent fan F side, safety valve (5) is provided with at vent fan G side roof part, vent fan valve (33) and tensimeter (35), vent fan G side bottom is provided with tapping valve (36).
4. a kind of edible fungi liquid strain according to claim 1 is cultivated and automatic vaccination device, and it is characterized in that: described Jie Jun device sterilising chamber sealing cover (13), be made up of two pot shape cover buckles, the above is silica gel pot together, and here is porose white steel pot.
CN201420588153.8U 2014-10-13 2014-10-13 A kind of edible fungi liquid strain is cultivated and automatic vaccination device Expired - Fee Related CN204097485U (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111909827A (en) * 2020-08-14 2020-11-10 浙江世荣农业开发有限公司 Edible fungus liquid fermentation strain culture equipment
CN115443852A (en) * 2022-06-10 2022-12-09 襄阳市农业科学院 Morel cultivation device and method

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111909827A (en) * 2020-08-14 2020-11-10 浙江世荣农业开发有限公司 Edible fungus liquid fermentation strain culture equipment
CN115443852A (en) * 2022-06-10 2022-12-09 襄阳市农业科学院 Morel cultivation device and method
CN115443852B (en) * 2022-06-10 2023-11-14 襄阳市农业科学院 Morchella cultivation device and method

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