CN203968983U - A kind of cyst roundworm second instar larvae hatching apparatus - Google Patents
A kind of cyst roundworm second instar larvae hatching apparatus Download PDFInfo
- Publication number
- CN203968983U CN203968983U CN201420307946.8U CN201420307946U CN203968983U CN 203968983 U CN203968983 U CN 203968983U CN 201420307946 U CN201420307946 U CN 201420307946U CN 203968983 U CN203968983 U CN 203968983U
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- Prior art keywords
- container
- sporangiocyst
- instar larvae
- cyst roundworm
- hatching apparatus
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Abstract
The utility model discloses a kind of cyst roundworm second instar larvae hatching apparatus, comprise that top is with the container of opening, vessel port is combined with lid, on lid, be equipped with sporangiocyst and hold pipe, the bottom opening that sporangiocyst holds pipe extends in container and is covered with screen cloth, container is transparent material, and container bottom is with graticule line.The utility model container bottom is transparent and draw and to have graticule line, be convenient to container to move to the counting that carries out cyst roundworm second instar larvae under microscope, and the hatching situation of the interior larva of real-time monitoring of containers, simplified the operating procedure in counting process, make the use of device more convenient.
Description
Technical field
The utility model relates to culture of nematodes technical field, relates in particular to a kind of hatching apparatus of cyst roundworm second instar larvae.
Background technology
Cyst roundworm (Heterodera spp.) is in the world important settling down property plant endoparasitism nematode, in China, all there is the harm of soy bean cyst roundworm (H.glycines) in nearly all northern soybean producing region, its disease symptom main manifestations be infected soybeans they grow slowly, blade yellow, root system is undeveloped and the root nodule that forms is little.And in wheat belt, found the distribution at 16 province's municipalization cereal cyst nematodes (H.avenae).Cyst roundworm is soil-borne disease, and it is propagated very strong.
Experience 4 times and cast off a skin the whole history of life of cyst roundworm.First, first-instar young (First-stage juveniles, J1s) casts off a skin into J2s in the ovum of sporangiocyst; J2s, cries again and infects larva, is cyst roundworm very important instar larvae in the history of life, and J2s infects host's root induction and forms and take food site, i.e. plasomidum; J2s obtains nutriment from plasomidum, and polypide is expanded and casts off a skin and develops into J3s; J3s is again casted off a skin and is developed into J4s (J4 male worm or female worm) at development later stage; J4s polypide continues to grow expands and casts off a skin into male worm or female worm adult; Female worm adult breaks through host's root tissue epidermis, but head is still fixed on and takes food that site Cell relay is continuous to be taken food; Male worm adult dissociate root complete mating with female worm; After mating completes, worm's ovum starts to form in female polypide; After female worm death, its polypide is transformed into the sporangiocyst of the firm and tool protectiveness of quality, protection worm's ovum wherein.How obtaining efficiently pure cyst roundworm second instar larvae is the prerequisite of research cyst roundworm.
The hatching m of traditional cyst roundworm second instar larvae is mainly to utilize common funnel, built-in multi-layer filter paper or lens wiping paper, funnel is placed on shelf, funnel bottom is connected to one with the emulsion tube of tongs, to pour funnel into the liquid of cyst roundworm worm's ovum, under room temperature, wait for two days, take tongs away, can collect the cyst roundworm second instar larvae of emulsion tube below.
Notification number is that the utility model patent of CN202979928U discloses a kind of Plant nematode list egg capsule hatching apparatus, comprise culture dish, double-deck paper handkerchief, two annulus that are threaded and 200 object gauzes, two annulus that are threaded are shelved in culture dish, this gauze clip is between two annulus that are threaded, and bedding is at interior annulus downside, double-deck paper handkerchief is located on the gauze of hollow of interior annulus.This utility model delicate structure, easy to use.
Notification number is that the utility model patent of CN203087305U discloses a kind of root-knot nematode incubator, the cuboid main body and the silicone tube that comprise hollow, main body end face is provided with infundibulate circular hole, a side of main body is provided with recovery valve, in the cavity of main body, one end is connected with infundibulate circular hole bottom, the other end is connected with recovery valve, and the side of main body is provided with water inlet and delivery port, and water inlet and delivery port all communicate with the inner chamber of main body.This utility model is simple to operate, is easy to thermostatic control, reclaims root-knot nematode second instar larvae convenient, firm, is difficult for pouring down or forth solution.
Although, the hatching that above-mentioned three kinds of hatching apparatus can be realized nematode obtains second instar larvae, and above-mentioned two utility model patents have all been done corresponding improvement on the basis of conventional funnel method principle, but above-mentioned hatching apparatus is when the larva quantity of the new hatching of statistics, all need to take out water containing second instar larvae as for just adding up on slide, complicated operation, is not easy to the observation that cyst roundworm is hatched situation in real time, and may affect statistical accuracy.
Summary of the invention
The purpose of this utility model is for above-mentioned weak point of the prior art, and a kind of cyst roundworm second instar larvae hatching apparatus that is easy to observe hatching situation, statistics larva quantity is provided.
A cyst roundworm second instar larvae hatching apparatus, comprises that top is with the container of opening, and described opening is combined with lid, on lid, be equipped with sporangiocyst and hold pipe, the bottom opening that sporangiocyst holds pipe extends in container and is covered with screen cloth, and described container is transparent material, and container bottom is with graticule line.
Cyst roundworm second instar larvae hatching apparatus is designed to the container with opening, and coordinates with lid, can make whole device be convenient to dismounting and cleaning.
Container adopts transparent material and in bottom, draws graticule line and can directly container be moved to the counting that carries out cyst roundworm second instar larvae under microscope, understand in real time the hatching situation of larva in container, and needn't take out the aqueous solution containing second instar larvae, be placed on slide and count again, can simplify the operating procedure of adding up larva quantity in hatching process, thereby realize the simplification of whole hatching process operation.
As preferably, the sidewall of described container is light screening material, and container side wall and container bottom are structure as a whole or removably connect, and can reduce extraneous intensity of illumination, avoids the inhibition of illumination to the growth of cyst roundworm second instar larvae.
Described lid is with installing hole, and described sporangiocyst holds the seal of tube and discloses and be located at installing hole place.
Described sporangiocyst holds pipe and comprises two sections, and epimere is for exceeding lid summit portion, and hypomere is for being in below, lid bottom surface part.The hypomere height that sporangiocyst holds pipe will affect the height of water in container, and the too high meeting of the water surface causes whole device to move difficulty, and the too low volume that can reduce the whole aqueous solution of the water surface, thereby the growth of second instar larvae is impacted.As preferably, it is container height 1/2～3/4 that sporangiocyst holds the height of pipe hypomere.
The edge of lid is bent downwardly and extends to form support portion, and the height of support portion is greater than the height of described hypomere.When using microscope to carry out second instar larvae counting, needing that first lid and sporangiocyst are held to pipe together takes out and is positioned on desktop, the support portion of lid is highly greater than because of it height that sporangiocyst holds pipe hypomere, can avoid sporangiocyst to hold pipe contacts with desktop, cause sporangiocyst to hold sporangiocyst in pipe and polluted, affect hatching process.
The top that sporangiocyst holds pipe is provided with seal cover, can seal whole device, and it is isolated from the outside, and effectively prevents from polluting.
The cross section that described sporangiocyst holds pipe from top to bottom dwindles gradually; Described sporangiocyst holds pipe for falling truncated cone shape, and cross section is circular.
The order number of described screen cloth is 200 orders.Due to the setting of screen cloth mesh diameter, second instar larvae can be entered in container by screen cloth, sporangiocyst is stayed sporangiocyst and is held in pipe because particle diameter is greater than screen cloth mesh diameter, thereby realizes the separated of sporangiocyst and second instar larvae.
Described screen cloth is bonded and fixed at the bottom opening place that sporangiocyst holds pipe.By bonding mode, making screen cloth be fixed on more firmly sporangiocyst holds on pipe.
Compared with prior art, the utlity model has following advantage:
(1) container bottom of apparatus of the present invention is transparent and draw and to have graticule line, be convenient to container to move to the counting that carries out cyst roundworm second instar larvae under microscope, the hatching situation of larva in monitoring of containers, has simplified the operating procedure in counting process in real time, makes the use of device more convenient.
(2) apparatus of the present invention adopt lid to coordinate with container, and at the top that sporangiocyst holds pipe, seal cover are set, and can avoid cyst roundworm unit cell capsule to be exposed in air and are polluted.
Accompanying drawing explanation
Fig. 1 is the structural representation of the utility model cyst roundworm second instar larvae hatching apparatus;
Fig. 2 is the structural representation of the hatching apparatus of cyst roundworm second instar larvae shown in Fig. 1 medium square line;
Fig. 3 is the structural representation of screen cloth in the hatching apparatus of cyst roundworm second instar larvae shown in Fig. 1.
Embodiment
Below in conjunction with accompanying drawing, the utility model cyst roundworm second instar larvae hatching apparatus is described in detail.
As shown in Figure 1, a kind of cyst roundworm second instar larvae hatching apparatus, comprises that top is with the hydrostatic column 1 of opening and the lid 2 coordinating with opening.Container 1 is transparent material, and container 1 bottom is with graticule line 6 (as Fig. 2), and what graticule line 6 formed is that size is the grid of 0.5cm * 0.5cm.The shape of container 1 can be square other shapes such as grade, and the sidewall of container 1 also can adopt light screening material, and the bottom of the sidewall of container 1 and container 1 can be structure as a whole, and also can dismantle connection.
The end face middle part of lid 2 is with installing hole, and the sealing of installing hole place is inserted with sporangiocyst and holds pipe 3, and its bottom opening extends in container 1.Sporangiocyst holds pipe 3 for falling frustoconical configuration, and cross section is circular.Sporangiocyst holds pipe 3 and also can be other structures, and as positive truncated cone shape, cylindrical etc., cross section also can be square or polygon.Sporangiocyst holds pipe 3 and comprises two sections, and epimere is for exceeding lid 2 summit portions, and hypomere is the part that is in lid 2 belows, bottom surface, and the height of hypomere is 1/2～3/4 of container 1 height.The top that sporangiocyst holds pipe 3 epimeres is provided with seal cover 4.The edge of lid 2 is bent downwardly and extends to form support portion 5, and the height of support portion 5 is greater than the height that sporangiocyst holds pipe 3 hypomeres.The bottom opening place that sporangiocyst holds pipe 3 is covered with screen cloth 7 (as Fig. 3), and screen cloth 7 is bonded and fixed at the bottom opening place that sporangiocyst holds pipe 3, and the order number of screen cloth 7 is 200 orders.
The course of work of above-mentioned cyst roundworm second instar larvae hatching apparatus is as follows:
First cyst roundworm second instar larvae hatching apparatus is carried out to autoclaving, and the monospore capsule of broken cyst roundworm, the sporangiocyst after fragmentation is positioned over sporangiocyst and holds in pipe 3, and covers seal cover 4, prevents from polluting.At the appropriate water of the interior injection of container 1, make the water surface not have sporangiocyst to hold the sporangiocyst of pipe on 3 bottom screen clothes 7, then device is placed in to aseptic culture case cultivates.Because cyst roundworm second instar larvae has hydrotaxis, after the second instar larvae of hatching dissociates from broken sporangiocyst, can enter in container 1 with the aqueous solution.Regularly the sporangiocyst hatching situation in container 1 is observed, during observation, lid 2 is held to pipe 3 together with sporangiocyst and together take off, be positioned on desktop, the support portion 5 of lid 2 supports lid 2 and sporangiocyst holds pipe 3, avoids it to contact with desktop, is polluted.Container 1 is placed in to micro-Microscopic observation, and the second instar larvae of the 6 pairs of hatchings of graticule line by container 1 bottom counts, to understand the hatching situation of sporangiocyst.When the complete submergence sporangiocyst of liquid level of the container 1 interior aqueous solution does not hold the sporangiocyst in pipe 3, carry out moisturizing, until finish whole hatching process.
Claims (10)
1. a cyst roundworm second instar larvae hatching apparatus, comprise that top is with the container of opening, it is characterized in that, described opening is combined with lid, on lid, be equipped with sporangiocyst and hold pipe, the bottom opening that sporangiocyst holds pipe extends in container and is covered with screen cloth, and described container is transparent material, and container bottom is with graticule line.
2. cyst roundworm second instar larvae hatching apparatus as claimed in claim 1, is characterized in that, the sidewall of described container is light screening material, and container side wall and container bottom are structure as a whole or removably connect.
3. cyst roundworm second instar larvae hatching apparatus as claimed in claim 1, is characterized in that, described lid is with installing hole, and described sporangiocyst holds the seal of tube and is plugged in installing hole place.
4. cyst roundworm second instar larvae hatching apparatus as claimed in claim 1, is characterized in that, described sporangiocyst holds pipe and comprises two sections, and epimere is for exceeding lid summit portion, and hypomere is for being in below, lid bottom surface part; The height of hypomere is 1/2～3/4 of container height.
5. cyst roundworm second instar larvae hatching apparatus as claimed in claim 4, is characterized in that, the edge of lid is bent downwardly and extends to form support portion, and the height of support portion is greater than the height of described hypomere.
6. cyst roundworm second instar larvae hatching apparatus as claimed in claim 1, is characterized in that, the top that sporangiocyst holds pipe is provided with seal cover.
7. cyst roundworm second instar larvae hatching apparatus as claimed in claim 1, is characterized in that, the cross section that described sporangiocyst holds pipe from top to bottom dwindles gradually.
8. cyst roundworm second instar larvae hatching apparatus as claimed in claim 7, is characterized in that, described sporangiocyst holds pipe for falling truncated cone shape, and cross section is circular.
9. cyst roundworm second instar larvae hatching apparatus as claimed in claim 1, is characterized in that, the order number of described screen cloth is 200 orders.
10. cyst roundworm second instar larvae hatching apparatus as claimed in claim 1, is characterized in that, described screen cloth is bonded and fixed at the bottom opening place that sporangiocyst holds pipe.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420307946.8U CN203968983U (en) | 2014-06-10 | 2014-06-10 | A kind of cyst roundworm second instar larvae hatching apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420307946.8U CN203968983U (en) | 2014-06-10 | 2014-06-10 | A kind of cyst roundworm second instar larvae hatching apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203968983U true CN203968983U (en) | 2014-12-03 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420307946.8U Expired - Fee Related CN203968983U (en) | 2014-06-10 | 2014-06-10 | A kind of cyst roundworm second instar larvae hatching apparatus |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106035238A (en) * | 2016-05-30 | 2016-10-26 | 中国科学院东北地理与农业生态研究所 | Nematode culture apparatus and simple breeding method for test entomopathogenic nematodes |
-
2014
- 2014-06-10 CN CN201420307946.8U patent/CN203968983U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106035238A (en) * | 2016-05-30 | 2016-10-26 | 中国科学院东北地理与农业生态研究所 | Nematode culture apparatus and simple breeding method for test entomopathogenic nematodes |
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| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20141203 Termination date: 20210610 |
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| CF01 | Termination of patent right due to non-payment of annual fee |