CN210226600U - Plant root knot nematode hatching collection device - Google Patents
Plant root knot nematode hatching collection device Download PDFInfo
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- CN210226600U CN210226600U CN201921099463.2U CN201921099463U CN210226600U CN 210226600 U CN210226600 U CN 210226600U CN 201921099463 U CN201921099463 U CN 201921099463U CN 210226600 U CN210226600 U CN 210226600U
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- Prior art keywords
- cavity
- knot nematode
- water
- collecting
- gauze layer
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- 241000243785 Meloidogyne javanica Species 0.000 title claims abstract description 30
- 241000196324 Embryophyta Species 0.000 title claims abstract description 26
- 230000012447 hatching Effects 0.000 title claims abstract description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 58
- 239000007788 liquid Substances 0.000 claims description 8
- 239000000463 material Substances 0.000 claims description 3
- 239000012780 transparent material Substances 0.000 claims description 3
- 241000244206 Nematoda Species 0.000 abstract description 23
- 239000012535 impurity Substances 0.000 abstract description 5
- 238000005119 centrifugation Methods 0.000 abstract description 2
- 238000000746 purification Methods 0.000 abstract description 2
- 239000008187 granular material Substances 0.000 abstract 1
- 241000238631 Hexapoda Species 0.000 description 5
- 238000000926 separation method Methods 0.000 description 5
- 239000001963 growth medium Substances 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 102000002322 Egg Proteins Human genes 0.000 description 2
- 108010000912 Egg Proteins Proteins 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 210000004681 ovum Anatomy 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 208000035143 Bacterial infection Diseases 0.000 description 1
- 244000017020 Ipomoea batatas Species 0.000 description 1
- 235000002678 Ipomoea batatas Nutrition 0.000 description 1
- 244000280244 Luffa acutangula Species 0.000 description 1
- 235000009814 Luffa aegyptiaca Nutrition 0.000 description 1
- 208000031888 Mycoses Diseases 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 241001560086 Pachyrhizus Species 0.000 description 1
- 208000037062 Polyps Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000000476 body water Anatomy 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000003071 parasitic effect Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000003449 preventive effect Effects 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
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Abstract
The utility model discloses a plant root-knot nematode hatching and collecting device, which comprises a collecting bottle and a gauze layer arranged in the collecting bottle, wherein the gauze layer is obliquely arranged in the middle of the collecting bottle and divides the inside of the collecting bottle into an upper cavity and a lower cavity; meshes of the gauze layer are 150-250 meshes, and an included angle of 30-60 degrees is formed between the gauze layer and the horizontal plane; the top of the upper cavity is provided with a bottle opening, and the bottom of the lower cavity is provided with a water outlet channel. The utility model discloses plant root knot nematode hatching collection device includes cavity and lower cavity, go up the cavity and keep apart through the gauze element layer that the slope set up with lower cavity, can ensure that only the root knot nematode after the hatching can pierce through gauze element layer and get into cavity down, and remaining ghost or other large granule impurity can not pass the gauze element layer after the egg piece hatching for only nematode and water in the cavity down, the nematode flows the back from exhalant canal along with water, through centrifugation, collect, can obtain the line worm body of purification.
Description
Technical Field
The utility model relates to a biological cultivation and purification technical field especially relate to a plant root tie nematode hatching collection device.
Background
Root-knot nematodes are a group of plant parasitic nematodes that cause roots of plants to form root knots, and the plants are more susceptible to other fungal and bacterial diseases. The roots of plants damaged by nematodes grow poorly, some plants generate tumors on the roots, similar to the root of the pachyrhizus, and some plants have swollen roots and do not grow capillary roots, and serious plants such as nematodes in the loofah lands can arch soil and are about as large as sweet potatoes. After the nematode damage occurs to the root system, the water and nutrients are basically not absorbed any more, the plant is easy to wilt, and the serious condition can cause the failure of production and harvest. Nematode damage occurs in many areas in China, and the current prevention work of nematodes is urgent.
Biological or preventive research on root-knot nematodes requires obtaining a large amount of root-knot nematodes, so that laboratory propagation of root-knot nematodes becomes an indispensable step in the early stage of root-knot nematode research. At present, the propagation of root-knot nematodes is mainly divided into living body propagation and in-vitro artificial culture medium propagation, and the in-vitro artificial culture medium propagation is divided into solid culture and liquid culture, wherein the solid culture and the living body propagation both need to clean the nematodes from cultures so as to be convenient for storage, transportation and quantitative use. At present, a water washing method is mainly adopted in the industrial production process of nematodes, the water consumption is large, impurities are more, and the method is difficult to be directly used for laboratory research.
SUMMERY OF THE UTILITY MODEL
In order to overcome the not enough of prior art, the utility model aims to provide a plant root knot nematode hatching collection device to overcome the cleaning process that current root knot nematode washs, collection device exists complicated, the ghost after the hatching is not thorough with the nematode separation and there are too many impurity scheduling problems in the nematode of collection.
The purpose of the utility model is realized by adopting the following technical scheme:
a plant root-knot nematode hatching and collecting device comprises a collecting bottle and a gauze layer arranged in the collecting bottle, wherein the gauze layer is obliquely arranged in the middle of the collecting bottle and divides the inside of the collecting bottle into an upper cavity and a lower cavity;
meshes of the gauze layer are 150-250 meshes, and an included angle of 30-60 degrees is formed between the gauze layer and the horizontal plane;
the top of the upper cavity is provided with a bottle opening, and the bottom of the lower cavity is provided with a water outlet channel.
Furthermore, the collecting bottle is made of transparent materials or semitransparent materials.
Furthermore, scale marks are arranged on the outer wall of the collecting bottle and used for measuring the water level in the collecting bottle.
Furthermore, the meshes of the gauze layer are 200 meshes, and the included angle of 45 degrees is formed between the gauze layer and the horizontal plane.
Furthermore, the top of the upper cavity is also provided with a water inlet channel, the bottom of the lower cavity is provided with a liquid collecting tank, and the top of the water outlet channel is connected with the bottom of the liquid collecting tank.
Furthermore, a first water valve is arranged on the water inlet channel, and a second water valve is arranged on the water outlet channel.
Compared with the prior art, the beneficial effects of the utility model reside in that:
the utility model discloses plant root knot nematode hatching collection device includes cavity and lower cavity, goes up the gauze element layer that cavity and lower cavity set up through the slope and keeps apart, goes up the bottleneck that cavity top set up and can the person of facilitating the use add the egg piece (the culture medium piece that contains root knot nematode ovum) to collection device in, and the water channel that the bottom of lower cavity set up is convenient for the nematode after the separation to follow water channel outflow, centrifugal collection. The meshes of the gauze layer are 150-250 meshes, so that only root-knot nematodes can penetrate through the gauze layer to enter the lower cavity, and incubated vacant shells or other large-particle impurities cannot penetrate through the gauze layer, so that only nematodes and water exist in the lower cavity, and the nematodes flow out of the water outlet channel along with the water, are centrifuged and collected, and then purified nematode bodies can be obtained and are directly used for experimental research. In addition, the water level is set to cover the gauze layer which is obliquely arranged on the part, namely the gauze layer is partially arranged on the water and partially arranged under the water, the nematodes tend to move towards the water, and then the nematodes are promoted to gather towards the obliquely downward side, and then the nematodes are promoted to pass through the gauze layer, so that the effect of separating the nematodes from the hatched empty shells is realized.
Drawings
Fig. 1 is a schematic structural view of a plant root-knot nematode hatching and collecting device according to an embodiment of the present invention;
FIG. 2 is a longitudinal sectional view of the plant root knot nematode hatching and collecting device shown in FIG. 1.
In the figure: 1. a collection bottle; 11. a bottle body; 12. a gauze layer; 13. a water inlet channel; 14. a liquid collecting tank; 2. an opening; 3. and a water outlet channel.
Detailed Description
The present invention will be further described with reference to the accompanying drawings and the detailed description, and it should be noted that the embodiments or technical features described below can be arbitrarily combined to form a new embodiment without conflict.
Example 1
As shown in fig. 1, the plant root-knot nematode hatching and collecting device is an embodiment of the present invention. This root knot nematode hatching collection device includes cylindric receiving flask 1, and the top of receiving flask 1 is provided with opening 2 and the lid supporting with the opening, can add the egg piece (the culture medium piece that contains the root knot nematode ovum) in this receiving flask 1 through opening 2, also can pass through lid shutoff opening 2 after adding, prevents that the polyp from revealing or other outside pollutants from resulting in the egg piece to be contaminated. The bottom of the collecting bottle 1 is provided with a water outlet channel 3, the separated worm body flows out from the water outlet channel along with water and is centrifugally collected, and the purified worm body can be directly used for experimental research.
As shown in fig. 2, it is a longitudinal sectional view of a plant root-knot nematode hatching and collecting device according to an embodiment of the present invention. The root knot nematode hatching and collecting device comprises a bottle body 11 and a gauze layer 12 arranged in the bottle body 11, wherein the gauze layer 12 is obliquely arranged in the middle of the bottle body 11, and the gauze layer 12 divides the interior of the bottle body 11 into an upper cavity and a lower cavity. Wherein, the mesh size of the gauze layer 12 is 150-250 meshes, and the included angle between the gauze layer 12 and the horizontal plane is 30-60 degrees. When the egg-shaped bottle is used, water is injected into the bottle body 11 through the opening 2 until the water level is lower than the lower half part of the gauze layer 12, and then the egg block is added into the upper cavity. At the moment, the worms in the upper cavity move towards the water source, so that a large number of worms are gathered above the gauze element layer 12 at a place with the water source, and the gathered nematodes further drive the nematodes to pass through the gauze element layer 12 and enter the lower cavity, so that the separation of the worms from hatched shells and other large granular impurities is realized. The gauze layer 12 is inclined by 30-60 degrees, so that the part of the water level exceeding the gauze layer 12 has larger volume, and the insects are induced to gather towards the position where the water level is over.
In a preferred embodiment, the bottle 11 is made of a transparent material or a translucent material. Therefore, when a user injects water into the bottle body 11, the user can observe and control the water level by naked eyes conveniently.
As a preferred embodiment, scale marks are arranged on the outer wall of the bottle body 11, and the scale marks are used for measuring the water level in the bottle body, so that a user can conveniently and accurately position the water level based on the scale marks.
As a preferred embodiment, the mesh size of the gauze layer 12 is 200 meshes, and at the moment, the gauze layer 12 has an optimal effect of separating the polypide, so that the polypide can be separated from the empty shell after hatching, the polypide can be ensured to rapidly enter the lower cavity, and the separation time is shortened. The gauze layer 12 and the horizontal plane form an included angle of 45 degrees, and the gauze layer 12 corresponding to the inclined angle can promote the polypide to be rapidly gathered and further enter the lower cavity downwards.
In a preferred embodiment, the top of the upper chamber (i.e., the top of the bottle body 11) is further provided with a water inlet channel 13, so that a user can conveniently fill water into the bottle body 11 through the water inlet channel 13. The bottom of the lower cavity (i.e. the bottom of the bottle body 11) is provided with a liquid collecting tank 14 and a water outlet channel 3, and the insect body-water mixture in the lower cavity can be discharged through the water outlet channel 3, for example, the insect body can be collected in a centrifuge tube and collected by means of centrifugation of the centrifuge tube. The liquid collecting groove 14 is arranged at the bottom of the bottle body 11, so that the insect bodies in the lower cavity can be conveniently collected and further discharged through the water outlet channel 3 communicated with the lower part of the liquid collecting groove.
In a preferred embodiment, the water inlet channel 12 is provided with a first water valve, and similarly, the water outlet channel 3 is provided with a second water valve. The flow of water inlet and outlet can be effectively controlled by the arranged first water valve and the second water valve, and the whole process of separating the worm bodies can be conveniently controlled by an experimenter.
Example 2
Example 2 differs from example 1 in that: the collection bottle 1 may also be of other shapes, for example cubic. The collecting bottle 1 can also be barrel-shaped, namely the upper part is open, a user can place the collecting bottle in a clean environment for insect body separation, and the water level can also be directly observed from the upper part.
The above embodiments are only preferred embodiments of the present invention, and the protection scope of the present invention cannot be limited thereby, and any insubstantial changes and substitutions made by those skilled in the art based on the present invention are all within the protection scope of the present invention.
Claims (6)
1. The plant root-knot nematode hatching and collecting device is characterized by comprising a collecting bottle and a gauze layer arranged in the collecting bottle, wherein the gauze layer is obliquely arranged in the middle of the collecting bottle and divides the inside of the collecting bottle into an upper cavity and a lower cavity;
meshes of the gauze layer are 150-250 meshes, and an included angle of 30-60 degrees is formed between the gauze layer and the horizontal plane;
the top of the upper cavity is provided with a bottle opening, and the bottom of the lower cavity is provided with a water outlet channel.
2. The plant root knot nematode hatching and collecting device of claim 1, wherein the collecting bottle is made of a transparent material or a translucent material.
3. The plant root knot nematode hatching and collecting device of claim 2, wherein scale marks are arranged on the outer wall of the collecting bottle and used for measuring the water level in the collecting bottle.
4. The plant root knot nematode hatching and collecting device of claim 3 wherein the mesh of said gauze layer is 200 mesh and said gauze layer forms an angle of 45 ° with the horizontal plane.
5. The plant root knot nematode hatching and collecting device of claim 4, wherein the top of the upper cavity is further provided with a water inlet channel, the bottom of the lower cavity is provided with a liquid collecting tank, and the top of the water outlet channel is connected with the bottom of the liquid collecting tank.
6. The plant root knot nematode hatching and collecting device of claim 5, wherein a first water valve is disposed on the water inlet channel, and a second water valve is disposed on the water outlet channel.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921099463.2U CN210226600U (en) | 2019-07-12 | 2019-07-12 | Plant root knot nematode hatching collection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921099463.2U CN210226600U (en) | 2019-07-12 | 2019-07-12 | Plant root knot nematode hatching collection device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN210226600U true CN210226600U (en) | 2020-04-03 |
Family
ID=69991080
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201921099463.2U Expired - Fee Related CN210226600U (en) | 2019-07-12 | 2019-07-12 | Plant root knot nematode hatching collection device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN210226600U (en) |
-
2019
- 2019-07-12 CN CN201921099463.2U patent/CN210226600U/en not_active Expired - Fee Related
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200403 |
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| CF01 | Termination of patent right due to non-payment of annual fee |