CN206783686U - Carbon dioxide cell incubator removes mould device automatically - Google Patents

Abstract

Animal physiology technical field is the utility model is related to, the carbon dioxide cell incubator that a kind of killing ability using copper to mould removes mould automatically is disclosed and removes mould device automatically.It includes degerming case, expansion box is set on the outside of described degerming case one end, asepwirator pump is provided with above expansion box, asepwirator pump is connected by venthole with expansion box, degerming case inner space is sequentially provided with pressurizing tank, jet orifice plate, degerming plate from the bottom up, degerming plate is made up of copper material, pressurizing tank connects with expansion box, matrix distribution has some fumaroles on jet orifice plate, jet orifice plate rims fix airtight connection with degerming case side wall, jet orifice plate upper surface forms tank with degerming case side wall, and tank is interior filled with the running water for being totally submerged degerming plate；Degerming plate lower surface forms degerming plate cavity of resorption, degerming plate upper surface and degerming case roof lower surface with jet orifice plate upper surface and forms degerming plate epicoele.

Description

Carbon dioxide cell incubator automatic mold removal device

technical field

The utility model relates to an automatic mold removal device for a carbon dioxide cell culture box, which belongs to the technical field of animal physiology.

Background technique

The carbon dioxide (CO2) incubator is to simulate a growth environment similar to cells and tissues in organisms in the incubator box. The incubator requires a stable temperature of 37°C, a stable CO2 level of 5%, and a constant pH value: 7.2 -7.4, a higher relative saturation humidity of 95%, a device for in vitro culture of cells and tissues, widely used in cell, tissue culture and the cultivation of some special microorganisms, common in animal physiology, cell dynamics research, Collection of mammalian cell secretions, carcinogenic or toxicological effects of various physical and chemical factors, research and production of antigens, culture of hybridoma cells to produce antibodies, in vitro fertilization IVF, stem cells, tissue engineering, drug screening and other research fields. The carbon dioxide incubator can provide the most accurate and stable control of temperature, carbon dioxide concentration and humidity, can effectively prevent microbial contamination in the incubator, and can regularly eliminate contamination to protect research results and prevent sample loss. The carbon dioxide incubator has two heating methods: air jacket heating and water jacket heating. The microprocessor control system is the operating system to maintain the steady state of temperature, humidity and CO2 concentration in the incubator. The use of micro-processing control system and other functional accessories such as high and low temperature automatic adjustment and alarm device, CO2 alarm device, password protection settings, etc., makes the operation and control of the carbon dioxide incubator very simple. For example: LEEC's PID microprocessor touch screen control system, which can strictly control the concentration of gas and reduce its loss to an extremely low level, so as to ensure a constant culture environment, and to ensure that the temperature in the box is accurate during the long-term culture process, and There are liquid crystal display, graphical process monitoring, intervention event recording, etc. In addition, the alarm system is also indispensable. It allows you to know the situation in the incubator in time and respond to it, thereby minimizing the loss and ensuring the continuity of the experiment. Some incubators have sound/light alarm devices, which will automatically alarm when the temperature change reaches ±0.5°C, or the CO2 concentration changes up to ±5%. Some have an alarm display function for abnormal CO2 concentration; These devices are all designed for the convenience of users and to reduce the tedious and boring experimental process. Carbon dioxide concentration can be measured by infrared sensor IR or thermal conductivity sensor TC. Humidity in the box is a very important but often neglected factor for cultivation work. Maintaining a sufficient humidity level and having a fast enough humidity recovery rate, such as after opening and closing the door, can ensure that the cultivation will not be caused by excessive drying. fail. At present, most carbon dioxide incubators generate moisture through the evaporation of the humidification plate, and the relative humidity level can reach about 95%, but the humidity recovery speed is very slow after opening the door. Try to choose an incubator with a large humidity evaporation area, because the larger the humidity evaporation area, the easier it is to reach the maximum relative saturated humidity and the shorter the humidity recovery time after opening and closing the door. Contamination is a major factor leading to cell culture failure. Manufacturers of carbon dioxide incubators have designed a variety of different devices to reduce and prevent contamination. The main way is to minimize the areas and surfaces where microorganisms can grow, and combine Automatic decontamination device to effectively prevent pollution. For example, in view of the fact that CO2 incubators are sometimes accompanied by mold growth during use, in order to ensure that the incubator is free from contamination and ensure the biological cleanliness of the instrument box, some companies have developed and designed CO2 incubators with ultraviolet disinfection functions; A company designed a HEPA high-efficiency filter to filter the air in the incubator and remove 99.97% of particles above 0.3um; in addition, the automatic high-temperature hot air sterilization device can make the temperature in the incubator reach a high temperature such as 200°C to kill all polluting microorganisms. Even high-temperature-resistant microorganisms such as spores, these devices are more secure for cell culture. During use, although there are some above-mentioned devices for preventing mold pollution, these devices should be able to reduce and prevent pollution according to the instructions, but sometimes, for unknown reasons, the carbon dioxide cell incubator still produces more mold, so Existing devices cannot completely remove the mold produced in the cell culture process, which often brings undue trouble to the experiment, and often even makes the entire experiment have to be redone due to mold contamination. Therefore, how to solve the mold growth in the existing carbon dioxide cell incubator, and better reduce and prevent the occurrence of pollution has become a major problem that needs to be solved urgently. After a lot of experiments and long-term exploration, the applicant found that putting copper-containing coins The humidification tray of the carbon dioxide cell incubator can better prevent mold pollution. Later, it was verified that putting copper wire or a sterilization plate made of copper sheet directly in the humidification tray has a better antibacterial effect. Therefore, use the suction pump to suck the gas in the carbon dioxide cell incubator, and transport it to the copper sterilization plate, and use the copper's ability to kill mold to automatically remove the mold. A utility model of a carbon dioxide cell incubator An automatic mold removal device is necessary.

Utility model content

In order to overcome the problem that the existing carbon dioxide cell incubator can't reduce and prevent pollution when there are many molds in the incubator, the utility model provides a method of using a suction pump to suck the gas in the carbon dioxide cell incubator and transport it to the copper On the high-quality sterilization board, the carbon dioxide cell incubator automatically removes the mold by using the ability of copper to kill the mold to automatically remove the mold.

The technical solution adopted by the utility model to solve the technical problem is: the carbon dioxide cell culture box automatically removes mold device, including a sterilization box, an air storage box is arranged outside one end of the sterilization box, and an air suction pump is arranged above the air storage box. The suction pump communicates with the air storage box through the air outlet. The internal space of the sterilization box is provided with a pressurized box, an air jet hole plate, and a sterilization plate in sequence from bottom to top. The sterilization plate is made of copper. The gas box is connected, and there are a number of jet holes distributed in a matrix on the jet orifice plate. The edge of the jet orifice plate is fixed and airtightly connected with the side wall of the sterilization box. The plate is completely submerged in tap water; the lower surface of the sterilizing plate and the upper surface of the air jet hole plate form the lower cavity of the sterilizing plate, and the upper surface of the sterilizing plate and the lower surface of the top wall of the sterilizing box form a cavity on the sterilizing plate.

The sterilization box is in the shape of a cuboid, with a length of 25-30 cm, a width of 20-30 cm, and a height of 5-10 cm; 30 centimeters and a height of 1-5 millimeters; the booster box is in the shape of a cuboid with a length of 25-30 centimeters, a width of 20-30 centimeters and a height of 1-2 centimeters.

The gas storage box is in the shape of a cuboid, with a length of 5-10 cm, a width of 20-30 cm, and a height of 5-10 cm.

The aspirator is in the shape of a cuboid, with a length of 10-15 centimeters, a width of 5-10 centimeters and a height of 5-10 centimeters.

The suction pump comprises a suction motor, an intake pipe connected to the suction motor, an air outlet, and a power cord of the suction pump. cord plug.

The air intake pipe is in an inverted L shape as a whole, and the air inlet is the horizontal end opening of the air intake pipe; the vertical lower end of the air intake pipe communicates with the suction pump.

The bottom wall of the sterilizing box, the side wall of the sterilizing box, the gas storage box, the air intake pipe, and the air jet orifice are made of plastic, glass, aluminum alloy or stainless steel.

The jet holes distributed in a matrix on the jet hole plate are conical jet holes, the jet hole outlets of the conical jet holes are located on the upper surface of the jet hole plate, and the jet hole air inlets of the conical jet holes are located under the jet hole plate. On the surface, the size of the air outlet of the air jet hole is smaller than the size of the air inlet of the air jet hole, and an air jet cavity is formed between the air outlet of the air jet hole and the air inlet of the air jet hole, and the rest of the physical part of the air jet hole plate is a tapered air jet orifice plate body.

The sterilizing board is composed of criss-crossing sterilizing board walls, which stand vertically in the water tank and are made of copper. They are vertically and horizontally connected by the sterilizing plate buckle seam, the bactericidal plate buckle seam opening of the sterilizing plate wall arranged vertically faces upward, the bactericidal plate buckle seam opening of the sterilizing plate wall arranged horizontally faces downward, and the vertical and horizontal sterilizing plate walls can be They are fastened together to form a grid connected up and down and surrounded by copper walls. The cavity surrounded by the sterilizing plate wall is the sterilizing plate cavity, and both ends of each sterilizing plate wall are connected with the side wall of the sterilizing box.

The length of the wall of the sterilization board is 25-30 cm or 20-30 cm, the width is 1-2 cm, and the thickness is 1-5 mm. The length of the seam of the sterilization board is 1-5 mm, and the width is 1-5 mm. The height is 0.5-1 cm, and the gap between two adjacent sterilizing plates is 1-3 cm.

Compared with the prior art, the beneficial effect of the utility model is: the utility model transports the gas in the carbon dioxide cell culture box to the gas storage box and the booster box through the suction pump, and then the gas enters the water tank through the jet orifice plate In contact with the copper sterilization plate, so as to kill and remove the mold in the gas, the purified gas re-enters the carbon dioxide cell incubator from the cavity of the sterilization plate; the utility model is simple to manufacture, strong in operability and low in cost , the effect is obvious.

Description of drawings

Fig. 1 is the overall structure schematic diagram of the automatic mold removal device of the carbon dioxide cell incubator;

Fig. 2 is a schematic diagram of the structure of the sterilization plate wall of the automatic mold removal device of the carbon dioxide cell incubator;

Fig. 3 Schematic diagram of the structure of the conical blowhole of the automatic mold removal device of the carbon dioxide cell incubator.

In the figure, 1 sterilization box, 2 booster box, 3 air storage box, 4 suction pump, 5 suction motor, 6 air inlet, 7 air intake pipe, 8 air outlet, 9 suction pump power cord, 10 suction Air pump power cord plug, 11 conical jet hole, 12 lower chamber of sterilization plate, 13 sterilization plate, 14 bottom wall of sterilization box, 15 water tank, 16 air injection orifice plate, 17 side wall of sterilization box, 18 cavity of sterilization plate , 19 Sterilization board wall, 20 Sterilization board cavity, 21 Sterilization board body, 22 Sterilization board buckle seam, 23 Jet hole air inlet, 24 Jet hole cavity, 25 Jet hole outlet, 26 Conical jet hole plate body.

detailed description

The utility model is described in further detail below in conjunction with the accompanying drawings and specific embodiments: As shown in Figures 1 to 3, the carbon dioxide cell incubator automatically removes mold devices, including a sterilization box 1, and a storage tank is arranged outside one end of the sterilization box 1. Air box 3, air suction pump 4 is arranged above the air storage box 3, the air suction pump 4 communicates with the air storage box 3 through the air outlet 8, and the internal space of the sterilization box 1 is provided with a booster box 2, an air injection box, and Orifice plate 16, sterilization plate 13, sterilization plate 13 is made of copper material, pressurization box 2 is connected with gas storage box 3, and matrix distribution has some air injection holes on air injection orifice plate 16, and the edge of air injection orifice plate 16 and sterilization The box side wall 17 is fixed and airtightly connected, and the upper surface of the air jet hole plate 16 forms a water tank 15 with the side wall 17 of the sterilization box. 1-2 centimeters; The lower surface of the sterilizing plate 13 and the upper surface of the air jet orifice plate 16 form the lower cavity 12 of the sterilizing plate, and the upper surface of the sterilizing plate 13 and the lower surface of the top wall of the sterilizing box form the cavity 20 on the sterilizing plate.

Described sterilization box 1 is cuboid shape, and length is 25-30 centimetre, and width is 20-30 centimeter, and height is 5-10 centimeter; 20-30 centimeters, and a height of 1-5 millimeters; the booster box 2 is in the shape of a cuboid, with a length of 25-30 centimeters, a width of 20-30 centimeters, and a height of 1-2 centimeters.

The gas storage box 3 is in the shape of a cuboid, with a length of 5-10 cm, a width of 20-30 cm, and a height of 5-10 cm.

Described suction pump 4 is cuboid shape, and length is 10-15 centimeters, and width is 5-10 centimeters, and height is 5-10 centimetres.

Described suction pump 4 comprises suction motor 5, the intake pipe 7 that is connected with suction motor 5 respectively, air outlet 8, suction pump power cord 9, and the opening of intake pipe 7 end is air inlet 6, and suction pump The suction pump power cord plug 10 that power cord 9 connects.

The air intake pipe 7 is in an inverted L shape as a whole, and the air inlet 6 is the horizontal end opening of the air intake pipe 7; the vertical lower end of the air intake pipe 7 communicates with the suction pump 4.

The bottom wall 14 of the sterilizing box, the side wall 17 of the sterilizing box, the gas storage box 3, the air intake pipe 7, and the air jet orifice 16 are made of plastic, glass, aluminum alloy or stainless steel.

The jet hole of matrix distribution on the described jet hole plate 16 is conical jet hole 11, and the jet hole air outlet 25 of conical jet hole 11 is positioned at the upper surface of jet hole plate 16, and the jet hole air inlet of conical jet hole 11 23 is positioned at the lower surface of jet orifice plate 16, and the size of jet hole air outlet 25 is smaller than the size of jet hole air inlet 23, and jet hole cavity 24 is formed between the jet hole air outlet 25 and the jet hole air inlet 23, and the air jet hole 24 of air jet orifice plate 16 The rest of the solid part is the tapered jet orifice body 26 .

Described sterilizing board 13 is made up of criss-crossing sterilizing board wall 19, and sterilizing board wall 19 vertically stands in the water tank 15, and sterilizing board wall 19 is made of copper material, and sterilizing board wall 19 comprises sterilizing board body 21 and sterilizing Plate buckle seams 22, the bacteria-eliminating plate walls 19 are vertically and horizontally connected through the bacteria-eliminating plate buckle seams 22, the openings of the bacteria-eliminating plate buckle seams 22 of the bacteria-eliminating plate walls 19 are arranged vertically, and the bacteria-eliminating plate buttons of the bacteria-eliminating plate walls 19 are arranged horizontally The opening of the slit 22 faces downward, and the longitudinal and transverse sterilizing plate walls 19 can be fastened together to form a grid connected up and down and surrounded by copper walls. The cavity surrounded by the sterilizing plate walls 19 is the sterilizing plate cavity 18, and each Both ends of the sterilizing plate wall 19 are connected with the side wall 17 of the sterilizing box.

The length of the sterilizing plate wall 19 is 25-30 cm or 20-30 cm, the width is 1-2 cm, and the thickness is 1-5 mm. The length of the sterilizing plate button seam 22 is 1-5 mm and the width is 1-5 mm millimeter, height is 0.5-1 centimetre, and the seam 22 of two adjacent sterilization board buckles is 1-3 centimeter apart.

In the utility model, the bottom wall 14 of the sterilization box and the side wall 17 of the sterilization box are made of plastic, glass, aluminum alloy or stainless steel with a thickness of 1-10 mm; Circular of millimeters, jet hole air inlet 23 is the circle that diameter is 1-5 millimeter; Intake pipe 7 cross-sections are rings, and internal diameter is 2-3 centimetre, inverted L-shaped air inlet pipe 7, the length in vertical direction It is 3-5 cm, and the length in the horizontal direction is 2-4 cm.

In the present utility model, the sterilizing plate body 21 is the main copper part of the sterilizing plate wall 19, and the sterilizing plate buckle 22 is a crack on the sterilizing plate wall 19; in addition, the sterilizing plate 13 in the present utility model can also be a whole formula structure.

In the utility model, the coil in the suction motor 5 is connected to the power line 9 of the suction pump; the suction end of the suction motor 5 is connected to the intake pipe 7, and the gas outlet is connected to the air outlet 8 through the cavity in the suction pump 4; Air outlet hole 8 is circular, and diameter is 2-3 centimetre, is positioned at the central position of air suction pump 4 and air storage box 3 connection surfaces, and air outlet hole 8 is the communication channel of air suction pump 4 and air storage box 3.

In the utility model, the structures and functions of the air suction pump 4, the air suction motor 5, and the power cord plug 10 of the air suction pump are the same as those of similar products on the existing market; Core power cord, the cross-section of the copper core is circular, with a diameter of 1-5 mm, one end of which is connected to the suction motor 5, and the other end is connected to the power cord plug 10 of the suction pump, which can deliver external power to the suction motor 5; The pump power cord plug 10 can be inserted into a known socket to lead external power into the suction pump power cord 9; the copper material can be pure copper or copper alloy.

The utility model is placed in the carbon dioxide cell culture box, and the gas in the carbon dioxide cell culture box is sucked into the gas storage box 3, the booster box 2, and the air injection hole plate 16 through the air suction pump 4 in turn, and enters through the conical air injection hole 11. The water tank 15 is in contact with the copper sterilization plate 13 in the water tank 15. After the mold in the gas is killed, the gas is released from the cavity 20 of the sterilization plate into the carbon dioxide cell incubator, thereby achieving automatic and efficient filtration and removal of carbon dioxide Purpose of mold in cell culture incubator.

In the utility model, the gas in the carbon dioxide cell culture box is continuously rushed into the booster box 2 under the continuous action of the suction pump 4, and the gas pressure in the booster box 2 increases. The opening of the lower end is larger than the opening of the upper end, so that the upper opening of the conical gas injection hole 11 is continuously ejected with higher pressure gas, and then the gas in the booster box 2 is sprayed into the lower cavity 12 of the sterilization plate, and the sterilization plate is also sprayed. The water in the lower chamber 12 cannot flow into the pressurized tank 2 along the conical air jet holes 11, where the suction volume of the air suction pump 4 is equal to the amount that can be discharged by all the conical air jet holes 11, so as to prevent the suction motor 5 from being hindered. Damaged; the edge of the air jet orifice plate 16 is fixed and airtightly connected with the side wall 17 of the sterilization box to ensure that all the gases in the pressurized box 2 are sprayed into the lower cavity 12 of the sterilization plate from the conical air jet hole 11, and at the same time, in the water tank 15 The water will not enter the pressurized tank.

The basic principles and main features of the present utility model and the advantages of the present utility model have been shown and described above. Those skilled in the industry should understand that the utility model is not limited by the above-mentioned embodiments. The above-mentioned embodiments and descriptions only illustrate the principle of the utility model. Without departing from the spirit and scope of the utility model, the utility model The new model also has various changes and improvements, and these changes and improvements all fall within the scope of the claimed utility model, and the protected scope of the utility model is defined by the appended claims and their equivalents.

Claims (10)

1. Automatic mold removal device for carbon dioxide cell incubator, characterized in that it includes a sterilization box (1), an air storage box (3) is arranged outside one end of the sterilization box (1), and an air storage box (3) is arranged above the air storage box (3). There is an air suction pump (4), and the air suction pump (4) communicates with the air storage box (3) through the air outlet (8), and the internal space of the sterilization box (1) is sequentially provided with a booster box (2) from bottom to top , air jet orifice plate (16), sterilization plate (13), the sterilization plate (13) is made of copper, the pressurization box (2) is connected with the gas storage box (3), and the matrix on the air jet orifice plate (16) A number of air jet holes are distributed, the edge of the air jet hole plate (16) is fixed and airtightly connected with the side wall (17) of the sterilization box, and the upper surface of the air jet hole plate (16) forms a water tank (15) with the side wall of the sterilization box (17). (15) is filled with running water that will completely immerse the sterilizing plate (13); ) upper surface and the lower surface of the top wall of the sterilization box form a cavity (20) on the sterilization plate. 2. The automatic mold removal device for a carbon dioxide cell incubator according to claim 1, characterized in that: the sterilization box (1) is in the shape of a cuboid with a length of 25-30 cm, a width of 20-30 cm, and a height of 5-10 cm; the air jet orifice (16) is in the shape of a cuboid, with a length of 25-30 cm, a width of 20-30 cm, and a height of 1-5 mm; the booster box (2) is in the shape of a cuboid, The length is 25-30 cm, the width is 20-30 cm, and the height is 1-2 cm. 3. The automatic mold removal device for a carbon dioxide cell incubator according to claim 1 or 2, characterized in that: the gas storage box (3) is in the shape of a cuboid with a length of 5-10 cm and a width of 20-30 cm. The height is 5-10 cm. 4. The automatic mold removal device for a carbon dioxide cell incubator according to claim 3, characterized in that: the air suction pump (4) is in the shape of a cuboid with a length of 10-15 cm, a width of 5-10 cm, and a height of 5-10 cm. 5. The automatic mold removal device for a carbon dioxide cell incubator according to claim 1, characterized in that: the air suction pump (4) includes an air suction motor (5), and air intake pipes connected to the air suction motor (5) respectively (7), air outlet (8), aspirator power cord (9), the end opening of the intake pipe (7) is the air inlet (6), and the aspirator power cord connected to the aspirator power cord (9) plug (10). 6. The automatic mold removal device for a carbon dioxide cell incubator according to claim 5, characterized in that: the air inlet pipe (7) is in an inverted L shape as a whole, and the air inlet (6) is in the horizontal direction of the air inlet pipe (7) The end is open; the vertical lower end of the air intake pipe (7) communicates with the suction pump (4). 7. The automatic mold removal device for a carbon dioxide cell incubator according to claim 1, characterized in that: the bottom wall (14) of the sterilization box, the side wall (17) of the sterilization box, the gas storage box (3), and the air intake pipe (7), jet orifice plate (16) is made of plastics, glass, aluminum alloy or stainless steel. 8. The automatic mold removal device for a carbon dioxide cell incubator according to claim 1, characterized in that: the air injection holes distributed in a matrix on the air injection orifice plate (16) are conical air injection holes (11), and the conical air injection holes ( 11) The jet hole air outlet (25) is located on the upper surface of the jet hole plate (16), and the jet hole air inlet (23) of the conical jet hole (11) is located on the lower surface of the jet hole plate (16). The air outlet hole (25) size is smaller than the air injection hole air inlet (23) size, and the air injection hole cavity (24) is formed between the air injection hole air outlet (25) and the air injection hole air inlet (23), and the air injection orifice plate (16) The remaining solid parts are conical jet orifice plate bodies (26). 9. The automatic mold removal device for a carbon dioxide cell incubator according to claim 1, characterized in that: the sterilization board (13) is composed of criss-crossing sterilization board walls (19), and the sterilization board walls (19) stand vertically In the water tank (15), the sterilizing board wall (19) is made of copper material, and the bactericidal board wall (19) comprises the sterilizing board body (21) and the germinating board buckle seam (22), and the sterilizing board wall (19) The rooms are connected vertically and horizontally through the sterilizing plate buckle (22), the sterilizing plate buckle (22) of the sterilizing plate wall (19) is arranged vertically upward, and the bactericidal plate buckle (22) of the sterilizing plate wall (19) is arranged horizontally ( 22) With the opening facing downwards, the vertical and horizontal sterilizing plate walls (19) can be fastened together to form a grid connected up and down and surrounded by copper walls, and the cavity surrounded by the sterilizing plate walls (19) is the sterilizing plate cavity (18), the two ends of each sterilizing plate wall (19) are all connected with the sterilizing box side wall (17). 10. The automatic mold removal device for a carbon dioxide cell incubator according to claim 9, characterized in that: the length of the sterilization board (19) is 25-30 cm or 20-30 cm, the width is 1-2 cm, and the thickness is 25-30 cm. 1-5 mm, the length of the sterilizing plate buckle (22) is 1-5 mm, the width is 1-5 mm, and the height is 0.5-1 cm, and the distance between two adjacent sterilizing plate buckles (22) is 1-5 mm. 3 cm.