CN206783686U - Carbon dioxide cell incubator removes mould device automatically - Google Patents

Abstract

Animal physiology technical field is the utility model is related to, the carbon dioxide cell incubator that a kind of killing ability using copper to mould removes mould automatically is disclosed and removes mould device automatically.It includes degerming case, expansion box is set on the outside of described degerming case one end, asepwirator pump is provided with above expansion box, asepwirator pump is connected by venthole with expansion box, degerming case inner space is sequentially provided with pressurizing tank, jet orifice plate, degerming plate from the bottom up, degerming plate is made up of copper material, pressurizing tank connects with expansion box, matrix distribution has some fumaroles on jet orifice plate, jet orifice plate rims fix airtight connection with degerming case side wall, jet orifice plate upper surface forms tank with degerming case side wall, and tank is interior filled with the running water for being totally submerged degerming plate；Degerming plate lower surface forms degerming plate cavity of resorption, degerming plate upper surface and degerming case roof lower surface with jet orifice plate upper surface and forms degerming plate epicoele.

Description

Carbon dioxide cell incubator removes mould device automatically

Technical field

It the utility model is related to a kind of carbon dioxide cell incubator and remove mould device automatically, belong to Animal physiology skill Art field.

Background technology

Carbon dioxide（CO2）Incubator is by simulating one similar cell of formation in incubator box body and being organized in life Growing environment in object, 37 DEG C of temperature that incubator to be aspired for stability, stable CO2 levels 5%, constant acid-base value pH value： 7.2-7.4, higher relative saturation humidity 95%, a kind of device of in vitro culture is carried out to cell, tissue, is widely used in The culture of cell, tissue cultures and some peculiar microorganisms, it is common in Animal physiology, cyto-dynamics research, mammal The collection of cell secreta, various physics, the carcinogenic or toxicological effect of chemical factor, the research of antigen and production, culture hybridization The research fields such as oncocyte production antibody, inseminatio externalis IVF, stem cell, organizational project, drug screening.CO2gas incubator Most precise and stable control can be provided temperature, gas concentration lwevel and humidity, can be to the microorganism pollution in incubator Effectively taken precautions against, and can periodically eliminate pollution, with Protective strategy achievement, prevent sample loss.Carbon dioxide culture Case has gas jacketing heat and water-jacket typ to heat two kinds of mode of heatings.Micro treatment control system is to maintain culture the temperature inside the box, humidity With the operating system of CO2 concentration stable states.Micro treatment control system and other various functions annex such as high/low temperatures are automatically adjusted and warned The utilization of reporting device, CO2 alarm devices, cryptoguard setting etc. so that the operation and control of CO2gas incubator are all very Simplicity.Such as：LEEC PID microprocessor touch screen control systems, it can strictly control the concentration of gas and is lost Extremely low level is down to, to ensure that culture environment is invariable, and the temperature inside the box is accurate during can guarantee that long-term cultivation, and has liquid Crystalline substance display, patterning process monitoring, Intervention Events record etc..In addition warning system is also indispensable that it can allow you to know in time The situation that incubator occurs, and make a response, so as to reduce loss to greatest extent, ensure the continuity of experiment.Some trainings Support case it is sound/light alarm device, temperature change reach ± 0.5 DEG C, or CO2 change in concentration reach ± 5% when, that is, understand automatic alarm；Have There is CO2 concentration abnormality alarm indication functions a bit；Some have low pressure, power alarm function.These devices are provided to conveniently User, designed with reducing cumbersome uninteresting experimentation.Gas concentration lwevel can pass through infrared sensor IR or heat Derivative sensor TC is measured.In case humidity be for training one it is extremely important however again through it is commonly overlooked because Element, maintaining enough humidity level and to have sufficiently fast humidity resume speed just to can guarantee that such as after switch gate will not be due to It is over-drying and cause culture fail.Current most CO2gas incubator is wet by the evaporation generation for the disk that is humidified Its caused relative humidity level of gas is up to 95% or so, but humidity resume speed is very slow after enabling.Moisture evaporated is selected as far as possible The big incubator of area, because moisture evaporated area is bigger, after more easily reaching maximum relative saturation humidity and switch gate The time that humidity is recovered is shorter.Pollution is to cause a principal element of cell culture failure, the manufacture of CO2gas incubator Business devise the generation that a variety of different devices go to reduce and prevent pollution, and its main path is all to try to reduction microorganism can With the region and surface of growth, and combine the generation that the device that decontaminates automatically effectively prevents from polluting.For example, in view of CO2 is trained Foster case is in use sometimes with fungus growth, to ensure incubator from polluting and ensureing the biology in instrument box Spatter property, the exploitation of some companies devise the CO2 incubators with ultraviolet disinfection function；Also company's design HEPA high efficiency filters Air in incubator can be filtered, may filter that the particle for more than the 0.3um for removing 99.97%；In addition, automatic high-temperature hot air sterilization Device can make the temperature inside the box reach a high temperature such as 200 DEG C so as to kill all contaminating microorganisms, or even the high temperature resistant microorganism such as gemma, These devices are more to have safety guarantee for cell culture.In use, mould is prevented in spite of some of the above The device of pollution, these devices should be able to reduce and prevent the generation of pollution according to explanation, still, sometimes, not know assorted Reason, carbon dioxide cell incubator still can produce more mould, so existing device can not be fully erased thin Caused mould in born of the same parents' incubation, often brings unnecessary trouble to experiment, many times even can allow whole experiment Have to re-start due to mould contamination.Therefore how to solve mould growth in existing carbon dioxide cell incubator, more That reduces and prevent well pollution occurs to turn into a great problem that urgent need solves, and gropes by many experiments and long-time, starts When it is found by the applicant that the coin of cupric is put into the humidification disk of carbon dioxide cell incubator, can preferably prevent that mould is dirty Dye, demonstrated again directly be put into copper wire or the degerming plate made of copper sheet, fungistatic effect are more preferable being humidified in disk later.So make With the gas in asepwirator pump withdrawing carbon dioxide cell culture incubator, it is transported on copper degerming plate, using copper to mould Killing ability reach the automatic purpose for removing mould, a kind of carbon dioxide cell incubator of utility model removes mould dress automatically It is necessary to put.

Utility model content

It can not reduce and prevent to pollute in the case of mould growth is more in existing carbon dioxide cell incubator to overcome The problem of generation, the utility model provides the gas in a kind of withdrawing carbon dioxide cell culture incubator using asepwirator pump, by it It is transported on copper degerming plate, the carbon dioxide cell incubator of mould is removed automatically certainly using killing ability of the copper to mould It is dynamic to remove mould device.

Technical scheme is used by the utility model solves its technical problem：Carbon dioxide cell incubator is removed automatically Mould device, including degerming case, degerming case one end outside set expansion box, asepwirator pump, asepwirator pump are provided with above expansion box Being connected by venthole with expansion box, degerming case inner space is sequentially provided with pressurizing tank, jet orifice plate, degerming plate from the bottom up, Degerming plate is made up of copper material, and pressurizing tank connects with expansion box, and matrix distribution has some fumaroles, fumarole edges of boards on jet orifice plate Edge and degerming case side wall fix airtight connection, and jet orifice plate upper surface and degerming case side wall form tank, filled with will remove in tank The running water that bacterium plate is totally submerged；Degerming plate lower surface forms degerming plate cavity of resorption, degerming plate upper surface with jet orifice plate upper surface Degerming plate epicoele is formed with degerming case roof lower surface.

The degerming case is rectangular shape, and length is 25-30 centimetres, and width is 20-30 centimetres, is highly 5-10 lis Rice；The jet orifice plate is rectangular shape, and length is 25-30 centimetres, and width is 20-30 centimetres, is highly 1-5 millimeters；Institute It is rectangular shape to state pressurizing tank, and length is 25-30 centimetres, and width is 20-30 centimetres, is highly 1-2 centimetres.

The expansion box is rectangular shape, and length is 5-10 centimetres, and width is 20-30 centimetres, is highly 5-10 centimetres.

The asepwirator pump is rectangular shape, and length is 10-15 centimetres, and width is 5-10 centimetres, is highly 5-10 centimetres.

The asepwirator pump includes aspiration motor, the air inlet pipe being connected respectively with aspiration motor, venthole, air-breathing pumping source Line, air inlet pipe distal opening are air inlet, the asepwirator pump power-line plug being connected with asepwirator pump power line.

The air inlet pipe generally inverted L-shaped, air inlet are the distal openings of air inlet pipe horizontal direction；Air inlet pipe vertical direction Lower end connects with asepwirator pump.

The degerming bottom wall, degerming case side wall, expansion box, air inlet pipe, jet orifice plate by plastics, glass, aluminium alloy or Stainless steel is made.

The fumarole of matrix distribution is conical fumarole on the jet orifice plate, the fumarole outlet of conical fumarole For hole position in the upper surface of jet orifice plate, the fumarole air inlet of conical fumarole is located at the lower surface of jet orifice plate, fumarole Venthole size is less than fumarole air inlet port dimensions, and jet vestibule, spray are formed between fumarole venthole and fumarole air inlet Remaining entity part of spiracular plate is tapered injection stomata plate body.

The degerming plate is made up of crisscross degerming wooden partition, and degerming wooden partition is vertically stood in tank, and degerming wooden partition is Copper material is made, and degerming wooden partition includes degerming plate body and degerming plate button stitches, vertical in length and breadth by degerming plate button seam between degerming wooden partition It is connected, the degerming plate button seam of the degerming wooden partition of longitudinal arrangement is opening up, the degerming plate button seam opening court of transversely arranged degerming wooden partition Under, the degerming wooden partition of vertical and horizontal can mutually snap together the grid to form that connection surrounding up and down is copper wall, degerming The cavity that wooden partition surrounds is degerming plate chamber, and every degerming wooden partition both ends are connected with degerming case side wall.

The degerming wooden partition length is 25-30 centimetres or 20-30 centimetres, and width is 1-2 centimetres, and thickness is 1-5 millimeters, is removed Bacterium plate button seam length is 1-5 millimeters, and width is 1-5 millimeters, is highly 0.5-1 centimetres, and two neighboring degerming plate button seam is at a distance of 1-3 Centimetre.

Compared with prior art, the beneficial effects of the utility model are：The utility model is by asepwirator pump by carbon dioxide After gas in cell culture incubator is transported to expansion box and pressurizing tank, gas is then passed through jet orifice plate into being removed with copper in tank Bacterium plate contacts, and is removed so as to which the mould in gas be killed, and purified gas reenters carbon dioxide from degerming plate epicoele In cell culture incubator；The utility model makes simply, and workable, cost is cheap, positive effect.

Brief description of the drawings

Fig. 1 is the overall structure diagram that carbon dioxide cell incubator removes mould device automatically；

Fig. 2 is the degerming wooden partition structural representation that carbon dioxide cell incubator removes mould device automatically；

Fig. 3 carbon dioxide cell incubators remove the taper blow hole structure schematic diagram of mould device automatically.

In figure, 1 degerming case, 2 pressurizing tanks, 3 expansion boxes, 4 asepwirator pumps, 5 aspiration motors, 6 air inlets, 7 air inlet pipe, 8 outlets Hole, 9 asepwirator pump power lines, 10 asepwirator pump power-line plugs, 11 conical fumaroles, 12 degerming plate cavity of resorptions, 13 degerming plates, 14 are removed Bacterium bottom wall, 15 tanks, 16 jet orifice plates, 17 degerming case side walls, 18 degerming plate chambers, 19 degerming wooden partitions, 20 degerming plate epicoeles, 21 Degerming plate body, 22 degerming plate button seams, 23 fumarole air inlets, 24 jet vestibules, 25 fumarole ventholes, 26 tapered injection spiracular plates Body.

Embodiment

The utility model is described in further detail with embodiment below in conjunction with the accompanying drawings：Such as Fig. 1 to Fig. 3 institutes Show, carbon dioxide cell incubator removes mould device, including degerming case 1 automatically, and gas storage is set on the outside of described degerming one end of case 1 Case 3, the top of expansion box 3 are provided with asepwirator pump 4, and asepwirator pump 4 is connected by venthole 8 with expansion box 3, the degerming inner space of case 1 from Under be up sequentially provided with pressurizing tank 2, jet orifice plate 16, degerming plate 13, degerming plate 13 is made up of copper material, pressurizing tank 2 and expansion box 3 Connect, matrix distribution there are some fumaroles on jet orifice plate 16, and closed company is fixed in the edge of jet orifice plate 16 with degerming case side wall 17 Connect, the upper surface of jet orifice plate 16 and degerming case side wall 17 form tank 15, filled with degerming plate 13 is totally submerged in tank 15 Running water, degerming 1-2 centimetres of 13 upper surface of plate of water surface distance of running water；The degerming lower surface of plate 13 and the upper surface of jet orifice plate 16 Form degerming plate cavity of resorption 12, the upper surface of degerming plate 13 and degerming case roof lower surface and form degerming plate epicoele 20.

The degerming case 1 is rectangular shape, and length is 25-30 centimetres, and width is 20-30 centimetres, is highly 5-10 lis Rice；The jet orifice plate 16 is rectangular shape, and length is 25-30 centimetres, and width is 20-30 centimetres, is highly 1-5 millimeters； The pressurizing tank 2 is rectangular shape, and length is 25-30 centimetres, and width is 20-30 centimetres, is highly 1-2 centimetres.

The expansion box 3 is rectangular shape, and length is 5-10 centimetres, and width is 20-30 centimetres, is highly 5-10 lis Rice.

The asepwirator pump 4 is rectangular shape, and length is 10-15 centimetres, and width is 5-10 centimetres, is highly 5-10 lis Rice.

The asepwirator pump 4 includes aspiration motor 5, the air inlet pipe 7 being connected respectively with aspiration motor 5, venthole 8, asepwirator pump Power line 9, the distal opening of air inlet pipe 7 are air inlet 6, the asepwirator pump power-line plug 10 being connected with asepwirator pump power line 9.

The air inlet pipe 7 generally inverted L-shaped, air inlet 6 are the distal openings of the horizontal direction of air inlet pipe 7；Air inlet pipe 7 is vertical Direction lower end connects with asepwirator pump 4.

The degerming bottom wall 14, degerming case side wall 17, expansion box 3, air inlet pipe 7, jet orifice plate 16 by plastics, glass, Aluminium alloy or stainless steel are made.

The fumarole of matrix distribution is conical fumarole 11 on the jet orifice plate 16, the jet of conical fumarole 11 Hole venthole 25 is located at the upper surface of jet orifice plate 16, and the fumarole air inlet 23 of conical fumarole 11 is located at jet orifice plate 16 Lower surface, the size of fumarole venthole 25 is less than the size of fumarole air inlet 23, fumarole venthole 25 and jet gas inlet hole Jet vestibule 24 is formed between mouth 23, remaining entity part of jet orifice plate 16 is tapered injection stomata plate body 26.

The degerming plate 13 is made up of crisscross degerming wooden partition 19, and degerming wooden partition 19 is vertically stood in tank 15, is removed Bacterium wooden partition 19 is made up of copper material, and degerming wooden partition 19 includes degerming plate body 21 and degerming plate button seam 22, passes through between degerming wooden partition 19 Degerming plate button seam 22 is vertically connected in length and breadth, and the degerming plate button seam 22 of the degerming wooden partition 19 of longitudinal arrangement is opening up, transversely arranged to remove The degerming plate button seam 22 of bacterium wooden partition 19 is opening down, and the degerming wooden partition 19 of vertical and horizontal mutually can snap together to be formed It is the grid of copper wall around lower connection, the cavity that degerming wooden partition 19 surrounds is degerming plate chamber 18, every degerming both ends of wooden partition 19 It is connected with degerming case side wall 17.

The length of degerming wooden partition 19 is 25-30 centimetres or 20-30 centimetres, and width is 1-2 centimetres, and thickness is 1-5 millimeters, It is 1-5 millimeters that degerming plate button, which stitches 22 length, and width is 1-5 millimeters, is highly 0.5-1 centimetres, two neighboring degerming plate button seam 22 At a distance of 1-3 centimetres.

In the utility model, the degerming bottom wall 14, degerming case side wall 17 by the plastics of thickness 1-10 millimeters, glass, Aluminium alloy or stainless steel are made；The fumarole venthole 25 be a diameter of 0.01-0.05 millimeters circle, fumarole air inlet 23 be the circle of a diameter of 1-5 millimeters；The cross section of air inlet pipe 7 is annulus, and internal diameter is 2-3 centimetres, the air inlet pipe 7 of inverted L-shaped, vertically The length in direction is 3-5 centimetres, and the length of horizontal direction is 2-4 centimetres.

In the utility model, degerming plate body 21 is the main body copper part of degerming wooden partition 19, and degerming plate button seam 22 is degerming Crack on wooden partition 19；Degerming plate 13 in other the utility model can also be monolithic construction.

In the utility model, the coil in aspiration motor 5 is connected with asepwirator pump power line 9；The suction end of aspiration motor 5 connects Air inlet pipe 7, outlet side are connected by the cavity in asepwirator pump 4 with venthole 8；Venthole 8 is circular, a diameter of 2-3 centimetres, position In the middle position of asepwirator pump 4 and the joint face of expansion box 3, venthole 8 is the connected pore channel of asepwirator pump 4 and expansion box 3.

In the utility model, asepwirator pump 4, aspiration motor 5,26S Proteasome Structure and Function and the existing city of asepwirator pump power-line plug 10 Like product on field is identical；Asepwirator pump power line 9 is commercially available known bifilar copper core power line, and copper core cross section is circular, directly Footpath is 1-5 millimeters, and its one end connection aspiration motor 5, other end connection asepwirator pump power-line plug 10 can be defeated by extraneous power supply Give aspiration motor 5；Asepwirator pump power-line plug 10 is inserted into known socket, and extraneous power supply is imported into asepwirator pump power line 9； Copper material both can be fine copper material, also can be copper alloy.

The utility model is positioned in carbon dioxide cell incubator, and the gas in carbon dioxide cell incubator is via suction Air pump 4 is drawn into expansion box 3, pressurizing tank 2, jet orifice plate 16 successively, and enters tank 15 through conical fumarole 11, with water Copper degerming plate 13 in groove 15 contacts, and after the mould in gas is killed, gas is discharged into dioxy from degerming plate epicoele 20 again Change in carbon cell culture incubator, so as to reach the purpose that mould in carbon dioxide cell incubator is removed in automatically and efficiently filtering.

In the utility model, the gas in carbon dioxide cell incubator is in the presence of asepwirator pump 4 is constant, in a steady stream Constantly pour in pressurizing tank 2, gas pressure increases in pressurizing tank 2, because the conical lower ending opening of fumarole 11 is more than upper end Opening so that the gas that elevated pressures are not parked at the conical upper end open of fumarole 11 sprays, and then causes in pressurizing tank 2 Gas is sprayed in degerming plate cavity of resorption 12, also causes the water in degerming plate cavity of resorption 12 from flowing into pressurizing tank along conical fumarole 11 In 2, the inspiratory capacity of asepwirator pump 4 is equal with the amount that all conical fumaroles 11 can discharge here, in case aspiration motor 5 is hindered Hinder and damage；Airtight connection is fixed in the edge of jet orifice plate 16 with degerming case side wall 17, to ensure all gas in pressurizing tank 2 Degerming plate cavity of resorption 12 is sprayed into from conical fumarole 11, meanwhile, the water in tank 15 will not also enter in pressurizing tank.

The advantages of general principle and principal character of the present utility model and the utility model has been shown and described above.One's own profession The technical staff of industry is it will be appreciated that the utility model is not restricted to the described embodiments, described in above-described embodiment and specification Simply illustrate principle of the present utility model, on the premise of the spirit and scope of the utility model is not departed from, the utility model can also There are various changes and modifications, these changes and improvements are both fallen within claimed the scope of the utility model, and the utility model will Protection domain is sought by its equivalent thereof of appended claims.

Claims (10)

1. carbon dioxide cell incubator removes mould device automatically, it is characterised in that：It includes degerming case（1）, it is described degerming Case（1）Expansion box is set on the outside of one end（3）, expansion box（3）Top is provided with asepwirator pump（4）, asepwirator pump（4）Pass through venthole（8） With expansion box（3）Connection, degerming case（1）Inner space is sequentially provided with pressurizing tank from the bottom up（2）, jet orifice plate（16）, it is degerming Plate（13）, degerming plate（13）It is made up of copper material, pressurizing tank（2）With expansion box（3）Connection, jet orifice plate（16）Upper matrix distribution has Some fumaroles, jet orifice plate（16）Edge and degerming case side wall（17）Fixed airtight connection, jet orifice plate（16）Upper surface with Degerming case side wall（17）Form tank（15）, tank（15）It is interior filled with by degerming plate（13）The running water being totally submerged；Degerming plate （13）Lower surface and jet orifice plate（16）Upper surface forms degerming plate cavity of resorption（12）, degerming plate（13）Upper surface and degerming case roof Lower surface forms degerming plate epicoele（20）.

2. carbon dioxide cell incubator according to claim 1 removes mould device automatically, it is characterised in that：It is described to remove Bacterium case（1）For rectangular shape, length is 25-30 centimetres, and width is 20-30 centimetres, is highly 5-10 centimetres；The jet Orifice plate（16）For rectangular shape, length is 25-30 centimetres, and width is 20-30 centimetres, is highly 1-5 millimeters；The pressurizing tank （2）For rectangular shape, length is 25-30 centimetres, and width is 20-30 centimetres, is highly 1-2 centimetres.

3. carbon dioxide cell incubator according to claim 1 or 2 removes mould device automatically, it is characterised in that：Institute State expansion box（3）For rectangular shape, length is 5-10 centimetres, and width is 20-30 centimetres, is highly 5-10 centimetres.

4. carbon dioxide cell incubator according to claim 3 removes mould device automatically, it is characterised in that：The suction Air pump（4）For rectangular shape, length is 10-15 centimetres, and width is 5-10 centimetres, is highly 5-10 centimetres.

5. carbon dioxide cell incubator according to claim 1 removes mould device automatically, it is characterised in that：The suction Air pump（4）Including aspiration motor（5）, respectively with aspiration motor（5）The air inlet pipe of connection（7）, venthole（8）, air-breathing pumping source Line（9）, air inlet pipe（7）Distal opening is air inlet（6）, with asepwirator pump power line（9）The asepwirator pump power-line plug of connection （10）.

6. carbon dioxide cell incubator according to claim 5 removes mould device automatically, it is characterised in that：It is described enter Tracheae（7）Generally inverted L-shaped, air inlet（6）It is air inlet pipe（7）The distal opening of horizontal direction；Air inlet pipe（7）Under vertical direction End and asepwirator pump（4）Connection.

7. carbon dioxide cell incubator according to claim 1 removes mould device automatically, it is characterised in that：It is described to remove Bacterium bottom wall（14）, degerming case side wall（17）, expansion box（3）, air inlet pipe（7）, jet orifice plate（16）Closed by plastics, glass, aluminium Gold or stainless steel are made.

8. carbon dioxide cell incubator according to claim 1 removes mould device automatically, it is characterised in that：The spray Spiracular plate（16）The fumarole of upper matrix distribution is conical fumarole（11）, conical fumarole（11）Fumarole venthole （25）Positioned at jet orifice plate（16）Upper surface, conical fumarole（11）Fumarole air inlet（23）Positioned at jet orifice plate （16）Lower surface, fumarole venthole（25）Size is less than fumarole air inlet（23）Size, fumarole venthole（25）With Fumarole air inlet（23）Between formed jet vestibule（24）, jet orifice plate（16）Remaining entity part be tapered injection spiracular plate Body（26）.

9. carbon dioxide cell incubator according to claim 1 removes mould device automatically, it is characterised in that：It is described to remove Bacterium plate（13）By crisscross degerming wooden partition（19）Composition, degerming wooden partition（19）Vertically stand on tank（15）It is interior, degerming wooden partition （19）It is made up of copper material, degerming wooden partition（19）Including degerming plate body（21）Stitched with degerming plate button（22）, degerming wooden partition（19）Between Stitched by degerming plate button（22）It is vertically connected in length and breadth, the degerming wooden partition of longitudinal arrangement（19）Degerming plate button seam（22）It is opening up, Transversely arranged degerming wooden partition（19）Degerming plate button seam（22）It is opening down, the degerming wooden partition of vertical and horizontal（19）Can be mutual Snap together the grid to form that connection surrounding up and down is copper wall, degerming wooden partition（19）The cavity surrounded is degerming plate chamber （18）, every degerming wooden partition（19）Both ends with degerming case side wall（17）Connection.

10. carbon dioxide cell incubator according to claim 9 removes mould device automatically, it is characterised in that：It is described Degerming wooden partition（19）Length is 25-30 centimetres or 20-30 centimetres, and width is 1-2 centimetres, and thickness is 1-5 millimeters, degerming plate button seam （22）Length is 1-5 millimeters, and width is 1-5 millimeters, is highly 0.5-1 centimetres, two neighboring degerming plate button seam（22）At a distance of 1-3 Centimetre.