CN203646394U - Vitrification carrier - Google Patents
Vitrification carrier Download PDFInfo
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- CN203646394U CN203646394U CN201320633196.9U CN201320633196U CN203646394U CN 203646394 U CN203646394 U CN 203646394U CN 201320633196 U CN201320633196 U CN 201320633196U CN 203646394 U CN203646394 U CN 203646394U
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- straw
- carrying pipe
- frozen vector
- vitrified frozen
- pipe
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Abstract
The utility model provides a vitrification carrier, which comprises a bearing tube and a straw, wherein one end of the bearing tube is an open end, and the other end of the bearing tube is a closed end; one end of the straw is an open end, and the other end of the straw is a closed end; the open end of the bearing tube is connected with the open end of the straw; the bearing tube is made from a metal material. According to the vitrification carrier, the bearing tube is connected with the straw, so that liquid nitrogen can be prevented from entering the carrier; the bearing tube is made from the metal material, so that high heat conductivity coefficient is ensured, the cooling speed is high, the biotoxicity of a coolant can be reduced, and vitrification effects can be improved.
Description
Technical field
The utility model belongs to medical instruments field, particularly a kind of vitrified frozen vector.
Background technology
Vitrification is to utilize carrying tool; in temperature-fall period extremely fast; make the cryoprotector of high concentration change very high similar hyaloid solid-state of viscosity into; avoid the formation of intracellular ice crystal; reach the effect of cryoprotection, thereby significantly improve viability and the developmental potency of cell/tissue after freezing.Vitrification has simply, efficient, save time, the advantage such as safety, expense are few.
Vitrified frozen vector carries the object of cell/tissue while being freezing preservation, one of successful key factor of glass freezing is exactly to select suitable freezing carrier.Vitrified frozen vector mainly comprises at present: open drawing-down straw, closed drawing-down straw, electron microscope copper mesh, Pasteur's glass pipette, surface of solids method etc.Whether directly contact liquid nitrogen by it and be divided into open and closed type two classes.Open direct contact liquid nitrogen, frozen cooling is fast, but has biological pollution problem.Closed type is because needs conduct by tube wall; and closed type vitrified frozen vector is made up of PP, PE or Biocomposite material; these material thermal conductivity can be poor; so rate of temperature fall is slow; need to increase cryoprotection agent concentration; cause it to increase cell/tissue toxicity, affect glass freezing effect.
Utility model content
The purpose of this utility model is to provide a kind of vitrified frozen vector, for solve existing open type glass freezing carrier exist biological pollution and closed type vitrified frozen vector cooling rate slow, increased the problem of freezant bio-toxicity.
The utility model is to realize like this, a kind of vitrified frozen vector, comprise carrying pipe and straw, one end of described carrying pipe is openend, the other end is closing end, and one end of described straw is openend, and the other end is closing end, and the openend of described carrying pipe is connected with the openend of described straw, described carrying pipe adopts metal material.The carrying pipe of the vitrified frozen vector that the utility model provides is connected with straw, can stop liquid nitrogen to enter carrier inside, and carrying pipe adopts metal material, and thermal conductivity factor is large, and cooling rate is fast, can reduce the toxicity of freezant.
Further, described carrying pipe adopts argent, and described straw adopts polymer-based Biocomposite material.The thermal conductivity factor of argent is 412W/mK, is thermal conductivity factor maximum in metal, adopts argent to make carrying pipe, can make cooling rate the fastest, farthest reduces the bio-toxicity of freezant; Taking out from liquid nitrogen after vitrified frozen vector, because variations in temperature is excessive, if straw also adopts metal, will be difficult to open vitrified frozen vector, cannot take out the cell or tissue of preservation, so, straw adopts polymer-based Biocomposite material, polymer-based Biocomposite material hardness is little, can directly straw be sheared, thereby take out the cell or tissue of preserving very easily.
Further, described carrying pipe and described straw are threaded connection.Employing is threaded more common, and good sealing effect.
Further, described carrying pipe is connected by socket joint with described straw.It is to use the socket of pipeline to insert in the bellmouth of pipeline that socket joint connects, and first uses caulking material filleting after contraposition, then with encapsulant sealing, good sealing effect.
Further, the junction of described straw and described carrying pipe tightly wraps sealing with sealed membrane.Use sealed membrane to tightly wrap junction, can make vitrified frozen vector sealing effectiveness better.
Further, described carrying pipe is the high 2-7cm of being, tube wall external diameter is 0.3-1.0cm, the cylindrical tube that pipe thickness is 0.1mm-0.3mm, and described straw is the high cylindrical tube for 1-4cm.
The utility model provides a kind of vitrified frozen vector, comprise carrying pipe and straw, one end of described carrying pipe is openend, the other end is closing end, one end of described straw is openend, the other end is closing end, and the openend of described carrying pipe and the openend of described straw be connected, and described carrying pipe adopts metal material.Existing open type glass freezing carrier directly contacts liquid nitrogen, and frozen cooling is fast, but has biological pollution problem.Existing closed type vitrified frozen vector is because needs conduct by tube wall, and closed type vitrified frozen vector is made up of PP, PE or Biocomposite material, and these material heat conduction are very poor, so rate of temperature fall is slow, increased cryoprotector bio-toxicity.The carrying pipe of the vitrified frozen vector that the utility model provides is connected with straw, can stop liquid nitrogen to enter carrier inside, and carrying pipe adopts metal material, and thermal conductivity factor is large, and cooling rate is fast, can reduce the toxicity of freezant.
Accompanying drawing explanation
Fig. 1 is the vitrified frozen vector schematic diagram that the utility model the first embodiment to the four embodiment provide;
Fig. 2 is the vitrified frozen vector schematic diagram that the utility model the 5th embodiment to the ten embodiment provide;
Fig. 3 is the straw schematic diagram of the vitrified frozen vector that provides of the utility model the first embodiment to the ten embodiment;
Fig. 4 is the carrying pipe schematic diagram of the vitrified frozen vector that provides of the utility model the first embodiment to the ten embodiment.
Embodiment
Also by reference to the accompanying drawings the utility model is described in further detail below by specific embodiment.
The first embodiment:
Consult Fig. 1, Fig. 3, Fig. 4, a kind of vitrified frozen vector, comprise carrying pipe and straw, straw is the high cylindrical tube for 2cm, the tube wall external diameter end opening one end closure that is 0.46cm, carrying pipe be high for 4cm, tube wall external diameter be the cylindrical tube of 0.5cm, the pipe thickness end opening one end closure that is 0.2mm, straw and carrying pipe be threaded connection, and carrying pipe made by argent, straw is made up of PP.
The second embodiment:
Consult Fig. 1, Fig. 3, Fig. 4, a kind of vitrified frozen vector, comprise carrying pipe 1 and straw 2, straw 2 is high cylindrical tube for 2cm, the tube wall external diameter end opening one end closure that is 0.46cm, carrying pipe 1 be high for 4cm, tube wall external diameter be the cylindrical tube of 0.5cm, the pipe thickness end opening one end closure that is 0.2mm, carrying pipe 1 and straw 2 are threaded connection, and carrying pipe is made up of metallic copper, and straw is made up of PE.
The 3rd embodiment:
Consult Fig. 1, Fig. 3, Fig. 4, a kind of vitrified frozen vector, comprise carrying pipe and straw, straw is the high cylindrical tube for 2cm, the tube wall external diameter end opening one end closure that is 0.46cm, carrying pipe be high for 4cm, tube wall external diameter be the cylindrical tube of 0.5cm, the pipe thickness end opening one end closure that is 0.2mm, carrying pipe 1 and straw 2 are threaded connection, and carrying pipe is made up of argent, and straw is made up of polymer-based Biocomposite material.
The 4th embodiment:
Consult Fig. 1, Fig. 3, Fig. 4, a kind of vitrified frozen vector, comprise carrying pipe 1 and straw 2, straw 2 is high cylindrical tube for 2cm, the tube wall external diameter end opening one end closure that is 0.46cm, carrying pipe 1 be high for 4cm, tube wall external diameter be the cylindrical tube of 0.5cm, the pipe thickness end opening one end closure that is 0.2mm, carrying pipe 1 is connected by socket joint with straw 2, and carrying pipe 1 is made up of argent, and straw 2 is made up of polymer-based Biocomposite material.
The 5th embodiment:
Consult Fig. 2, Fig. 3, Fig. 4, a kind of vitrified frozen vector, comprise carrying pipe 1 and straw 2, straw 2 is high cylindrical tube for 2cm, the tube wall external diameter end opening one end closure that is 0.46cm, carrying pipe 1 be high for 4cm, tube wall external diameter be the cylindrical tube of 0.5cm, the pipe thickness end opening one end closure that is 0.2mm, carrying pipe 1 is connected by socket joint with straw 2, sealed membrane 3 circle on side seal outside for connecting portion, and carrying pipe 1 is made up of argent, and straw 2 is made up of polymer-based Biocomposite material.
The 6th embodiment:
Consult Fig. 2, Fig. 3, Fig. 4, a kind of vitrified frozen vector, comprise carrying pipe and straw, straw is the high cylindrical tube for 1cm, the tube wall external diameter end opening one end closure that is 0.28cm, carrying pipe be high for 2cm, tube wall external diameter be the cylindrical tube of 0.3cm, the pipe thickness end opening one end closure that is 0.1mm, carrying pipe 1 is connected by socket joint with straw 2, a sealed membrane circle on side seal outside for connecting portion, and carrying pipe is made up of argent, and straw is made up of polymer-based Biocomposite material.
The 7th embodiment:
Consult Fig. 2, Fig. 3, Fig. 4, a kind of vitrified frozen vector, comprise carrying pipe and straw, straw is the high cylindrical tube for 4cm, the tube wall external diameter end opening one end closure that is 0.94cm, carrying pipe be high for 7cm, tube wall external diameter be the cylindrical tube of 1cm, the pipe thickness end opening one end closure that is 0.3mm, carrying pipe 1 is connected by socket joint with straw 2, a sealed membrane circle on side seal outside for connecting portion, and carrying pipe is made up of argent, and straw is made up of polymer-based Biocomposite material.
The 8th embodiment:
Consult Fig. 2, Fig. 3, Fig. 4, a kind of vitrified frozen vector, comprise carrying pipe and straw, straw is the high cylindrical tube for 3cm, the tube wall external diameter end opening one end closure that is 0.46cm, carrying pipe be high for 5cm, tube wall external diameter be the cylindrical tube of 0.5cm, the pipe thickness end opening one end closure that is 0.2mm, carrying pipe 1 is connected by socket joint with straw 2, a sealed membrane circle on side seal outside for connecting portion, and carrying pipe is made up of argent, and straw is made up of polymer-based Biocomposite material.
The 9th embodiment:
Consult Fig. 2, Fig. 3, Fig. 4, a kind of vitrified frozen vector, comprise carrying pipe 1 and straw 2, straw 2 is high cylindrical tube for 3cm, the tube wall external diameter end opening one end closure that is 0.66cm, carrying pipe 1 be high for 5cm, tube wall external diameter be the cylindrical tube of 0.7cm, the pipe thickness end opening one end closure that is 0.2mm, carrying pipe 1 is connected by socket joint with straw 2, sealed membrane 3 circle on side seal outside for connecting portion, and carrying pipe 1 is made up of argent, and straw 2 is made up of polymer-based Biocomposite material.
The tenth embodiment:
Consult Fig. 2, Fig. 3, Fig. 4, a kind of vitrified frozen vector, comprise carrying pipe 1 and straw 2, straw 2 is high cuboid pipes for the bottom surface length of side of the 3cm end opening one end closure that is 0.46cm, carrying pipe 1 be high for 5cm, the bottom surface length of side be the cuboid pipe of 0.5cm, the pipe thickness end opening one end closure that is 0.2mm, carrying pipe 1 is connected by socket joint with straw 2, sealed membrane 3 circle on side seal outside for connecting portion, and carrying pipe 1 is made up of argent, and straw 2 is made up of polymer-based Biocomposite material.
The 11 embodiment: the application of the vitrified frozen vector that the 5th embodiment provides
The vitrified frozen vector sterilization that the 5th embodiment is provided, puts into the ovary tissue of 10 pieces of mouse after the carrying pipe 1 of the vitrified frozen vector after sterilization, and carrying pipe 1 is connected with straw 2, then tightly wraps sealing at interface with sealed membrane 3, freezing preservation.
Configuration thawing solution: 0.5M sucrose+10% hyclone+DPBS;
M16 medium: Sigma company.
After one month, from liquid nitrogen, take out rapidly vitrified frozen vector, cut off the straw 2 of vitrified frozen vector, take out Mouse Ovary Tissues, put into thawing solution, stop 30 minutes, move in M16 medium, in incubator, (37 ℃, 5%CO2) hatch the primordial follicle survival condition of 1 hour poststaining observation mouse.
Result shows, 10 pieces of Mouse Ovary Tissues of the freezing preservation of vitrified frozen vector that use the utility model the 5th embodiment provides obtain the primordial follicle of 460 mouse altogether after thawing and recovering, wherein survival has 420, survival rate is: 91.3%, be better than the 70-80% survival rate that most vitrifying carrier is reported.
The foregoing is only preferred embodiment of the present utility model, be not limited to the utility model, for a person skilled in the art, the utility model can have various modifications and variations.All within spirit of the present utility model and principle, any modification of doing, be equal to replacement, improvement etc., within all should being included in protection domain of the present utility model.
Claims (6)
1. a vitrified frozen vector, it is characterized in that: comprise carrying pipe and straw, one end of described carrying pipe is openend, the other end is closing end, one end of described straw is openend, the other end is closing end, and the openend of described carrying pipe and the openend of described straw be connected, and described carrying pipe adopts metal material.
2. vitrified frozen vector as claimed in claim 1, is characterized in that: described carrying pipe adopts argent.
3. vitrified frozen vector as claimed in claim 1, is characterized in that: described carrying pipe and described straw are threaded connection.
4. vitrified frozen vector as claimed in claim 1, is characterized in that: described carrying pipe is connected by socket joint with described straw.
5. vitrified frozen vector as claimed in claim 1, is characterized in that: the junction sealed membrane of described straw and described carrying pipe tightly wraps sealing.
6. vitrified frozen vector as claimed in claim 1, is characterized in that: described carrying pipe is the high 2-7cm of being, tube wall external diameter is 0.3-1.0cm, the cylindrical tube that pipe thickness is 0.1mm-0.3mm, and described straw is the high cylindrical tube for 1-4cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320633196.9U CN203646394U (en) | 2013-10-14 | 2013-10-14 | Vitrification carrier |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320633196.9U CN203646394U (en) | 2013-10-14 | 2013-10-14 | Vitrification carrier |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203646394U true CN203646394U (en) | 2014-06-18 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201320633196.9U Expired - Fee Related CN203646394U (en) | 2013-10-14 | 2013-10-14 | Vitrification carrier |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203646394U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105494316A (en) * | 2016-01-22 | 2016-04-20 | 四川大学华西第二医院 | Human division stage embryo and blastula closed frozen carrier and low-temperature preservation method |
| CN106577632A (en) * | 2016-12-05 | 2017-04-26 | 浙江大学 | Device for freeze preservation of human ovarian tissue under liquid nitrogen |
-
2013
- 2013-10-14 CN CN201320633196.9U patent/CN203646394U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105494316A (en) * | 2016-01-22 | 2016-04-20 | 四川大学华西第二医院 | Human division stage embryo and blastula closed frozen carrier and low-temperature preservation method |
| CN105494316B (en) * | 2016-01-22 | 2018-09-07 | 四川大学华西第二医院 | People's division stage embryo and the closed freezing carrier of blastaea and Cryopreservation |
| CN106577632A (en) * | 2016-12-05 | 2017-04-26 | 浙江大学 | Device for freeze preservation of human ovarian tissue under liquid nitrogen |
| CN106577632B (en) * | 2016-12-05 | 2020-03-27 | 浙江大学 | Device is deposited to freezing under human ovary tissue liquid nitrogen |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140618 Termination date: 20191014 |