CN203382764U - Cell proliferation bag and cell proliferation device - Google Patents
Cell proliferation bag and cell proliferation device Download PDFInfo
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- CN203382764U CN203382764U CN201320435347.XU CN201320435347U CN203382764U CN 203382764 U CN203382764 U CN 203382764U CN 201320435347 U CN201320435347 U CN 201320435347U CN 203382764 U CN203382764 U CN 203382764U
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Abstract
The utility model discloses a cell proliferation bag and a cell proliferation device, which belong to the technical field of cell proliferation. The proliferation bag comprises a bag body with a sealed edge, wherein a flow guide body is arranged on the bag body, the flow guide body is attached to the bottom surface of the bag body, the bottom surface side length or the diameter of the flow guide body is 0.5-20 cm, and the height of the flow guide body is 0.5-10 cm. The cell proliferation device comprises a tray and the cell proliferation bag arranged on the tray, wherein fixed rods are arranged at the edges of the two opposite sides of the bag body of the cell proliferation bag, and the fixed rods are arranged along the edge of the bag body; a clamping buckle connected with the fixed rods in a clamping manner is arranged on the tray; and a bulged flow guide body is arranged on a bearing surface contacted with the cell proliferation bag, of the tray, the bottom surface side length or the diameter of the flow guide body is 0.5-20 cm, and the height of the flow guide body is 0.5-10 cm. By arranging the flow guide body, a culture solution in the cell proliferation bag generates an upward guide flow while moving on a two-dimensional surface, thus achieving a purpose of uniformly mixing the culture solution under a small shearing force.
Description
Technical field
The utility model relates to the cell proliferation technical field, particularly relates to a kind of cell proliferation bag and cell proliferation device.
Background technology
Cell is the basic form of life, no matter the research of material is deep into to which kind of degree, and its impact on life is all represented in the cell levels aspect.In order to study the life problem and the application cell technology promotes the well-being of mankind, new cell technology emerges in an endless stream, and has embodied the modern biology technology in continuous development.
Perfect cell technique is a system, comprising the screening of cell strain, and the optimization of substratum, the improvement of equipment.In recent years, owing to having adopted new thinking of development, advanced cell proliferation processing method is particularly remarkable to the raising of working efficiency.Along with the modern material the reach of science, the various equipment for disposable cell technique constantly occurs, a kind ofly take the modern cell technique that the pocket type system is representative, for the mankind's health, contributing.
Modern cell process system, owing to having adopted disposable cell proliferation bag, has improved working efficiency greatly, has reduced total cost.But also there is defective place in the design of cell proliferation bag at present.In existing cell proliferation bag, in order to make cell proliferation, evenly in liquid system, to carry out, adopt rocks to reach the purpose mixed more.If in low frequency, mixed under motion by a small margin, although the shearing force produced is little, there is the inhomogeneous problem of stirring in nutrient solution; And if nutrient solution is mixed under high frequency, Large Amplitude Motion, although can stir, the shearing force produced is excessive, is unfavorable for cell proliferation.
As the cell proliferation bag employing soft mould material poor to the gas-barrier effect in the past, soft mould material has the not characteristic of choke that blocks water.The mode that this bag inside obtains oxygen relies on the passive mode exchange of naturally osmotic to carry out.The passive mode of naturally osmotic is difficult to reach the cell proliferation needs under conditions of high density.Cell proliferation bag does not in the past have the gas circuit connecting path, can't adopt oxygen supply mode initiatively to realize cell proliferation.
Cell proliferation bag in the past adopts the mode at the liquid flow path of bag side, at the equal direct contact liq below liquid level of these paths of actual cultivation conditions, can't connect gas circuit.
Summary of the invention
Based on this, the purpose of this utility model is to overcome the defect of prior art.
For achieving the above object, the utility model is taked following technical scheme:
A kind of cell proliferation bag, comprise the bag of edge seal; Described bag is provided with baffle, this baffle and the laminating of bag bottom surface, and the bottom surface length of side of baffle or diameter are 0.5-20cm, are highly 0.5-10cm.
Cell proliferation bag of the present utility model, setting by baffle, in the blending process of nutrient solution, only need low frequency, motion by a small margin, just can make the nutrient solution in the cell proliferation bag produce water conservancy diversion upwards when two-dimensional surface moves, make the nutrient solution in the cell proliferation bag realize three-dimensional motion, reach the purpose that realization mixes under less shearing force.This baffle can be arranged in the bottom film material of bag, also can be arranged at outside the bag film.And, owing to being provided with baffle, to the shape of bag also without restriction.In the utility model, baffle is the motion of liquid to be produced to the solid unit of the effect of making progress, therefore, its shape is not limit, cuboid, prismatoid, half sphere shape, cone-shaped body, Polygons, irregular multiaspect body etc. both can be adopted as, can be also strip, its cross section can be the shapes such as rectangle, trapezoidal, semicircle, trilateral, Polygons, but also is not limited to these.
In embodiment, the axial section of described baffle is trilateral therein.Be beneficial to when nutrient solution moves, nutrient solution is guided to top by the bag bottom.
In embodiment, described baffle is at least two, is uniformly distributed in bag therein.For example, when baffle is two, can place at 3-point line place, bag bottom surface; When baffle is three, can place in the dislocation of 3-point line place, bag bottom surface; Baffle is distributed in to the bag bottom, can makes nutrient solution more easily mix.
In embodiment, described bag is made by the soft mould material to the gas high-barrier therein, and this mould material reaches following index: temperature is 23 ℃, and the outer humidity relatively of bag is 50%, and when in bag, relative humidity is 90%, OTR oxygen transmission rate is less than 1ml/m
2/ day/atm; Temperature is 23 ℃, and when the inside and outside relative humidity of bag is 0%, the carbonic acid gas vapor permeability is less than 1ml/m
2/ day/atm; Temperature is 23 ℃, and the outer humidity relatively of bag is 0%, and when in bag, relative humidity is 100%, water vapour transmission rate is less than 2g/m
2/ day/atm.Make bag with the soft mould material that the gas superelevation is intercepted, can avoid on the one hand the gas fed to overflow, also can increase on the other hand the pressure in bag, thereby improve dissolved oxygen level, meet the cell proliferation needs under conditions of high density.
In embodiment, this upper face of bag body is provided with and is communicated with the first inside and outside transfer tube of bag therein, and the end that this first transfer tube is communicated with the bag outside connects the gas circuit pipe of air feed.Described gas circuit pipe end also is provided with the hydrophobic membrane gas filter that aperture is 0.01～0.45 μ m.Described gas circuit end also can be provided with aseptic pressure protect cap etc., for gnotobasis and the pressurize of on cell proliferation bag that maintains bag inside, and discharges gas when pressure is excessive, avoids bag to produce explosion.Utilize the tubular paired propagation bag of transfer tube and gas circuit to carry out initiatively supply air circuit, in the raising dissolved oxygen amount, avoid gas inlet to open below the liquid level of nutrient solution and the bubble of generation damages cell.
In embodiment, described bag top also is provided with and is communicated with the second inside and outside transfer tube of bag therein, and the end that this second transfer tube is communicated with the bag outside connects aseptic holdout device, can in non-clean environment, carry out at any time aseptic technique.Meet the aseptic technique requirement in the situations such as inoculation, results, feed supplement, sterile sampling, perfusion.
In embodiment, described the first transfer tube and/or the second transfer tube are provided with annular linking part outward, by this annular linking part stopping property, are fixed on bag therein.This fixed form has advantages of fixing firm, good leak tightness.
In embodiment, the place, both sides of the edge that described bag is relative is provided with fixed link therein, and this fixed link arranges along the bag edge.This fixed link can be relative at bag both sides arrange respectively two, also can arrange respectively four at bag two opposite sides facing each other, utilize this fixed link to keep the bag shape, avoid making propagation bag distortion, folding in carrying or motion.
In embodiment, also comprise pallet therein, described pallet is provided with the buckle with described fixed link clamping.Utilize the structure of buckle, bag is fixed on pallet, make bag be supported and protect in carrying or motion.The weight-bearing surface of this pallet can be plane, inclined-plane or curved surface, does not all affect the effect of pallet.Also be provided with handle on described pallet, conveniently move.This tray bottom also is provided with screw and clamp, pallet can be fixed on the motion platform of other equipment.
The utility model also provides a kind of cell proliferation device, comprises pallet and is placed in the cell proliferation bag on pallet, and the place, both sides of the edge that the bag of described cell proliferation bag is relative is provided with fixed link, and this fixed link arranges along the bag edge; Described pallet is provided with the buckle with described fixed link clamping; The weight-bearing surface that this pallet contacts with the cell proliferation bag is provided with protruding baffle, and the bottom surface length of side of this baffle or diameter are 0.5-20cm, is highly 0.5-10cm.When the cell proliferation bag is positioned on pallet, bag bottom surface and the laminating of pallet weight-bearing surface, make this propagation bag inner bottom part shape consistent with the pallet weight-bearing surface, this baffle plays the above-mentioned effect that is arranged at baffle on the cell proliferation bag, make the nutrient solution in the cell proliferation bag produce water conservancy diversion upwards when two-dimensional surface moves, reach the three-dimensional mixed uniformly purpose of nutrient solution.
Compared with prior art, the utlity model has following beneficial effect:
The cell proliferation bag that the utility model provides has solved that cell proliferation bag in the past in use exists is difficult for reaching three-dimensional mixed uniformly problem.By baffle is set, the three-dimensional motion of formation, thus be issued in less shearing force the purpose that nutrient solution mixes.And the mode of motion of on cell proliferation bag is without restriction, to the shape of bag also without restriction.
Cell proliferation bag of the present utility model also utilizes transfer tube and gas circuit pipe to carry out the active air feed to the propagation bag, and use the soft mould material that the gas superelevation is intercepted to make bag, can avoid on the one hand the gas fed to overflow, cause waste, also can increase on the other hand the pressure in bag, thereby the raising dissolved oxygen level, meet the cell proliferation needs under conditions of high density.
Adopt this cell proliferation bag, can on original condition basis, greatly improve the cell quantity in unit volume.As the cultivation to the T cell, can be issued in very high survival rate and surpass 8 * 10
7the cell density of cell/ml.And the effective working volume of this cell proliferation bag is 5%～50% of bag cumulative volume, just can realize the amplification of 10 times of volumes in a propagation bag, avoided the frequent trouble gone down to posterity that shifts in different culture vessels.Not only save the manpower consumed in cultivation and collection process, also reduced total cost simultaneously.
The cell proliferation bag that the utility model provides is pre-sterilized, fully airtight, disposable.The proposition of the cell proliferation device novelty that the utility model provides the cell proliferation scheme of oxygenation initiatively, multiple interfaces on the cell proliferation bag, be respectively used to ventilation, inoculation, results, feed supplement, sampling and perfusion culture etc., can in non-clean environment, carry out at any time aseptic technique.These not only save the manpower consumed in cultivation and collection process, and have also reduced overall cost when having reduced the operating environment requirement.
The accompanying drawing explanation
The cell proliferation bag structural representation that Fig. 1 is the utility model embodiment 1;
The vertical view that Fig. 2 is Fig. 1;
The side-view that Fig. 3 is Fig. 1;
Fig. 4 is transfer tube and bag fixed sturcture schematic diagram in embodiment 1;
Fig. 5 is embodiment 1 transfer joint pipe structure schematic diagram;
The sectional view that Fig. 6 is Fig. 5;
The vertical view that Fig. 7 is Fig. 5;
The upward view that Fig. 8 is Fig. 5;
Fig. 9 is support holder structure schematic diagram in embodiment 1;
The side-view that Figure 10 is Fig. 9;
Figure 11 is buckle and pallet annexation schematic diagram in embodiment 1;
Figure 12 is support holder structure schematic diagram in embodiment 2;
The side-view that Figure 13 is Figure 12;
Figure 14 is clamp and Elastic Bolts annexation schematic diagram in embodiment 2.
Wherein: 1. bag; 2. baffle; 3. the first transfer tube; 4. gas circuit pipe; 5. strainer; 6. the second transfer tube (6); 7. fixed link; 8. annular linking part; 9. pallet; 10. handle; 11. screw; 12. clamp; 13. buckle; 14. chute; 15. standing bolt; 16. fixed orifices; 17. strainer support bar; 18. Elastic Bolts; 19. retaining plate.
Embodiment
Describe the utility model in detail below in conjunction with the drawings and specific embodiments.
A kind of cell proliferation bag, as Figure 1-3, comprise the bag 1 of edge seal; Described bag 1 is provided with baffle 2, this baffle and the laminating of bag bottom surface, and the bottom surface diameter of this baffle is 8cm, is highly 5cm.
Described baffle bottom surface is circular, and its vertical cross section is trilateral, and this baffle is cone.
Described baffle is three, is uniformly distributed in bag bottom side one side, and at the bottom of bag, the dislocation of 3-point line place is placed.Described bag is made by the soft mould material that the gas superelevation is intercepted; As Figure 4-8, this upper face of bag body is provided with and is communicated with the first inside and outside transfer tube 3 of bag, and this first transfer tube 3 is two, and its end that is communicated with the bag outside connects the gas circuit pipe 4 of air feed respectively, is respectively gas feed and pneumatic outlet.Described gas inlet and outlet is equipped with the hydrophobic membrane filter 5 of 0.2 μ m, and described pneumatic outlet is provided with aseptic pressure protect cap, and when gaseous tension is greater than 0.5Bar, tin hat can be crossed gas.The soft mould material of gas high-barrier (the FLEXCLR Film of U.S. Flexbiosys company film) should reach following index to the barriering efficiency of gas: OTR oxygen transmission rate is less than 1ml/m
2/ day/atm (23 ℃, the 50%RH outside, 90%RH inboard); The carbonic acid gas vapor permeability is less than 1ml/m
2/ day/atm (23 ℃, 0%RH); Water vapour transmission rate is less than 2g/m
2/ day/atm (23 ℃, the 0%RH outside, 100%RH inboard).
Described bag top also is provided with and is communicated with the second inside and outside transfer tube 6 of bag, and this second transfer tube 6 is six, and its end that is communicated with the bag outside all connects aseptic holdout device.
Described the first transfer tube and the second transfer tube are provided with annular linking part 8 outward, by this annular linking part 8, are fixed on bag.
The place, both sides of the edge that described bag is relative is provided with fixed link 7, and this fixed link arranges along the bag edge.
Also comprise pallet 9, as shown in Figure 9, described pallet 9 is provided with the buckle 13 with described fixed link clamping.As shown in Figure 10-11, in the present embodiment, these buckle 13 cross sections be question mark "? " shape, by reaching fixing effect in the cavity that fixed link is fixed in to the formation of question mark shape.Also be provided with chute 14 on this buckle 13, standing bolt 15 is fixed in the fixed orifices 16 be located on pallet 9 through this chute 14, thereby limits the position of buckle 13.During use, first standing bolt 15 is unscrewed, fixed link 7 is positioned in the cavity that the question mark shape of buckle 13 forms, then make buckle 13 down slidings, block fixed link 7, tighten standing bolt 15, thereby fixed link 7 is fixed.
Also be provided with handle 10 on described pallet 9, also be provided with screw 11 and clamp 12 on this pallet.Described screw 11 arranges a plurality of, according to the position of fixing threaded hole on the motion platform with its coupling, selects applicable screw.
Cell proliferation bag and the cell proliferation bag in embodiment 1 of the present embodiment are basic identical, and difference is:
The rectangular parallelepiped that described baffle bottom surface is the length of side 10 * 0.5 * 0.5cm.This baffle is two, along bag end 3-point line, places.
As shown in figure 12, also comprise strainer support bar 17 on described pallet 9, this strainer support bar 17 is two, keeps at a certain distance away parallel fixed placement on pallet 9, gas filter 5 is placed on this strainer support bar 17, and the sebific duct at strainer 5 to first transfer tube places is kept upright.
As shown in Figure 13-14, described pallet 9 also comprises that Elastic Bolts 18(is as spring hitch etc.), the hole that this Elastic Bolts 18 is provided with through clamp 12, be resisted against on retaining plate 19, thereby pallet 9 be fixed in to motion platform.When pallet is taken off, carry a little on drawing after Elastic Bolts outward, can directly take off pallet.
Cell proliferation bag and the cell proliferation bag in embodiment 1 of the present embodiment are basic identical, and difference is: the rectangle that described baffle bottom surface is the length of side 20 * 5cm, the trilateral that its vertical cross section is high 3cm.This baffle is three, is distributed in the bag bottom, and at the bottom of bag, the dislocation of 3-point line place is placed.
A kind of cell proliferation device, comprise pallet and be placed in the cell proliferation bag on pallet, comprises pallet and be placed in the cell proliferation bag on pallet, and the place, both sides of the edge that the bag of described cell proliferation bag is relative is provided with fixed link, and this fixed link arranges along the bag edge; Described pallet is provided with the buckle with described fixed link clamping; The weight-bearing surface that this pallet contacts with the cell proliferation bag is provided with protruding baffle, and this baffle is diameter 3cm, the cone of height 2cm, and this baffle is 4, is distributed on the weight-bearing surface that pallet contacts with the cell proliferation bag.
Described bag is made by the soft mould material that the gas superelevation is intercepted; This upper face of bag body is provided with and is communicated with the first inside and outside transfer tube of bag, and this first transfer tube is two, and its end that is communicated with the bag outside connects the gas circuit pipe of air feed respectively, is respectively gas feed and pneumatic outlet.Described gas circuit pipe end also is provided with the hydrophobic membrane filter of 0.2 μ m.The soft mould material that the gas superelevation is intercepted should reach following index to the barriering efficiency of gas: OTR oxygen transmission rate is less than 1ml/m
2/ day/atm (23 ℃, the 50%RH outside, 90%RH inboard); The carbonic acid gas vapor permeability is less than 1ml/m
2/ day/atm (23 ℃, 0%RH); Water vapour transmission rate is less than 2g/m
2/ day/atm (23 ℃, the 0%RH outside, 100%RH inboard).
Described bag top also is provided with and is communicated with the second inside and outside transfer tube of bag, and this second transfer tube is six, and its end that is communicated with the bag outside all connects aseptic holdout device.
Embodiment 5CIK cell proliferation experiment
Cytokine induced kill cell (CIK) is carried out to proliferation test, investigate the impact of different cell proliferation bag on cell proliferation.
Gather normal people's peripheral blood 200ml, apply the liquid-tight degree gradient centrifugation of commercial Ficoll-Paque PREMIUM layering and isolate mononuclearcell (PBMC); Nutrient solution washing 2 times, obtain 3.0 * 10
8pBMC, purity is 90%.The RPMI1640 nutrient solution tune PBMC concentration that contains 10%AB type human serum of take is 1 * 10
6after cell/ml, be divided into isopyknic seven parts standby.
A group: Cultilife215 (0.3L) cell culture bags and GT-T610 (1.8L) cell bags that adopt Japanese TAKARA company.
A organizes cell growth method: after a group of PBMC cell injection Cultilife215 cell culture bags, the rear RPMI1640 nutrient solution by the proportioning cell mixing factor first above-mentioned separation obtained is filled into 50ml, folder closes entrance.Put into 37 ℃, 5%CO
2in incubator, cultivate.Within every 3 days, change liquid and expand bag once, after volume of culture surpasses 300ml, changing GT-T610 (1.8L) cell bags into, adding as required bag quantity.Harvested cell after cultivating 15 days.
B group: the AP10 rip current type bio-reactor that adopts initiative oxygen supply.
Connect AP10 bio-reactor and AP10 cell bags according to service requirements, with the CO of air and 5%
2gas is inflated until its expansion hardening the AP10 cell bags.Set pH 7.0, temperature is at 37 ℃, and dissolved oxygen (DO), 450%, shakes speed 30r/min.First the nutrient solution of 400ml is injected to the AP10 cell bags, after shaking 1 hour, then 2 parts of cells are injected to the AP10 cell bags.Keep continuous 0.3 liter of (lpm) active air feed of per minute.Cultivate harvested cell after 15 days.
C group: the cell proliferation bag that adopts the utility model embodiment 1.
D group: the cell proliferation bag that adopts the utility model embodiment 2.
E group: the cell proliferation bag that adopts the utility model embodiment 3.
F group: the cell proliferation bag that adopts the utility model embodiment 4.
C-F organizes cell growth method: the CO that at first uses air and 10%
2gas is inflated until its expansion hardening adds the RPMI1640 nutrient solution and clamps import and the egress filtering device the propagation bag.Shake and within 1 hour, make pH and temperature reach complete equipilibrium with the frequency of per minute 15 times.After breeding the one group of PBMC cell that adds above-mentioned separation to obtain in bag, open import and egress filtering device, change the CO of air and 5% into
2gas, keep continuous 0.3 liter of (lpm) active air feed of per minute.
Initial cell density is according to amount and the type decided of the factor of the composition of substratum and feed supplement and interpolation.A-F group all adds the interferon-γ that final concentration is 1000U/ml (IFN-γ) on the 1st day in cultivation; Within the 2nd day, add the CD3 monoclonal antibody (OKT3) that final concentration is 100ng/ml, the phytoh(a)emagglutinin (PHA) of the interleukin II of 1000U/ml (IL-2) and 100ug/ml, the granulocyte-macrophage colony stimutaing factor (GM-CSF) of the interleukin 4 of 10ng/ml (IL-4) and 1000U/ml; Then two groups of A, B added the fresh nutrient solution containing IL-21000U/ml every 2 days, and to adjust cell concn be 1 * 10
6/ ml; Cultivate harvested cell after 15 days, the harvested cell situation is as shown in table 1 below.
The cell situation that table 1. results obtain
| Group | Cultivate liquid measure | Cell density | Living cell rate | Total cellular score (individual) |
| The A group | 9L | 1.60×10 6cell/ml | 79% | 1.44×10 10 |
| The B group | 2L | 1.98×10 7cell/ml | 84% | 3.96×10 10 |
| The C group | 2L | 8.7×10 7cell/ml | 91% | 1.74×10 11 |
| The D group | 2L | 8.4×10 7cell/ml | 90% | 1.68×10 11 |
| The E group | 2L | 8.6×10 7cell/ml | 94% | 1.72×10 11 |
| The F group | 2L | 8.2×10 7cell/ml | 93% | 1.64×10 11 |
Test-results is as shown in Table 1:
1, the nutrient solution usage quantity of C-F group than the A group little 70%;
2, the cell density of C-F group has improved 10 times, has improved more than 4 times than B group than A group;
3, the cell proliferation multiple of C-F group has improved 10 times, B group than A group and has improved more than 4 times;
4, the cell proliferation bag of C-F group embodiment 1-4 can support 8.2 * 10
7the cell density that/ml is above.
In sum, the cell proliferation bag in compared to existing technology, the cell proliferation bag of the utility model embodiment 1-4 has advantages of and simplifies the operation, raises the efficiency, reduces costs when cell proliferation.
The above embodiment has only expressed several embodiment of the present utility model, and it describes comparatively concrete and detailed, but can not therefore be interpreted as the restriction to the utility model the scope of the claims.It should be pointed out that for the person of ordinary skill of the art, without departing from the concept of the premise utility, can also make some distortion and improvement, these all belong to protection domain of the present utility model.Therefore, the protection domain of the utility model patent should be as the criterion with claims.
Claims (9)
1. a cell proliferation bag, comprise the bag of edge seal; It is characterized in that, described bag is provided with baffle, this baffle and the laminating of bag bottom surface, and the bottom surface length of side of baffle or diameter are 0.5-20cm, are highly 0.5-10cm.
2. cell proliferation bag according to claim 1, is characterized in that, the axial section of described baffle is trilateral.
3. cell proliferation bag according to claim 1, is characterized in that, described baffle is at least two, is uniformly distributed in bag.
4. cell proliferation bag according to claim 1, is characterized in that, this upper face of bag body is provided with and is communicated with the first inside and outside transfer tube of bag, and this first transfer tube is communicated with the gas circuit pipe of an end connection air feed of bag outside.
5. cell proliferation bag according to claim 4, is characterized in that, described bag top also is provided with and is communicated with the second inside and outside transfer tube of bag, and the end that this second transfer tube is communicated with the bag outside connects aseptic holdout device.
6. cell proliferation bag according to claim 5, is characterized in that, described the first transfer tube and/or the second transfer tube are provided with annular linking part outward, by this annular linking part stopping property, is fixed on bag.
7. cell proliferation bag according to claim 1, is characterized in that, the place, both sides of the edge that described bag is relative is provided with fixed link, and this fixed link arranges along the bag edge.
8. cell proliferation bag according to claim 7, is characterized in that, also comprises pallet, and described pallet is provided with the buckle with described fixed link clamping.
9. a cell proliferation device, is characterized in that, comprises pallet and be placed in the cell proliferation bag on pallet, and the place, both sides of the edge that the bag of described cell proliferation bag is relative is provided with fixed link, and this fixed link arranges along the bag edge; Described pallet is provided with the buckle with described fixed link clamping; The weight-bearing surface that this pallet contacts with the cell proliferation bag is provided with protruding baffle, and the bottom surface length of side of this baffle or diameter are 0.5-20cm, is highly 0.5-10cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320435347.XU CN203382764U (en) | 2013-07-19 | 2013-07-19 | Cell proliferation bag and cell proliferation device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320435347.XU CN203382764U (en) | 2013-07-19 | 2013-07-19 | Cell proliferation bag and cell proliferation device |
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| CN203382764U true CN203382764U (en) | 2014-01-08 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103361271A (en) * | 2013-07-19 | 2013-10-23 | 广州中玺医疗科技有限公司 | Cell proliferation bag and cell proliferation device |
| WO2017107837A1 (en) * | 2015-12-23 | 2017-06-29 | 上海吉凯基因科技有限公司 | Fully automated continuous cell culture system |
| CN107922906A (en) * | 2015-08-07 | 2018-04-17 | 株式会社日立制作所 | disposable cell culture apparatus and culture bag |
-
2013
- 2013-07-19 CN CN201320435347.XU patent/CN203382764U/en not_active Withdrawn - After Issue
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103361271A (en) * | 2013-07-19 | 2013-10-23 | 广州中玺医疗科技有限公司 | Cell proliferation bag and cell proliferation device |
| CN107922906A (en) * | 2015-08-07 | 2018-04-17 | 株式会社日立制作所 | disposable cell culture apparatus and culture bag |
| WO2017107837A1 (en) * | 2015-12-23 | 2017-06-29 | 上海吉凯基因科技有限公司 | Fully automated continuous cell culture system |
| US10934513B2 (en) | 2015-12-23 | 2021-03-02 | Shanghai GenBase Biotechnology Co., Ltd. | Fully automated continuous cell culture system |
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