CN203353360U - High-pressure vessel for plant seedling chromosome reduplication treatment - Google Patents
High-pressure vessel for plant seedling chromosome reduplication treatment Download PDFInfo
- Publication number
- CN203353360U CN203353360U CN 201320389239 CN201320389239U CN203353360U CN 203353360 U CN203353360 U CN 203353360U CN 201320389239 CN201320389239 CN 201320389239 CN 201320389239 U CN201320389239 U CN 201320389239U CN 203353360 U CN203353360 U CN 203353360U
- Authority
- CN
- China
- Prior art keywords
- pressure
- pressure bottle
- end cover
- doubles
- valve
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
A high-pressure vessel for plant seedling chromosome reduplication treatment comprises a cylinder body and upper and lower end covers connected with the cylinder body, and is characterized in that the upper end cover is hermetically connected with the cylinder body through a flange, and the lower end cover is integrally welded with the cylinder body; and a window is arranged on the cylinder body, a temperature detecting device and a pressure detecting device are arranged inside the bottom of the high-pressure vessel, and used for sending out temperature signals and pressure signals to a PLC (programmable logic controller), and the PLC is used for controlling opening and closing of an air intake sequencing valve and an air exhaust sequencing valve.
Description
Technical field
The utility model relates to a kind of reaction vessel, specifically refers to a kind of for realize the high-pressure bottle of chromosome doubling function by laughing gas HIGH PRESSURE TREATMENT plant plant.
Background technology
Chromosome is the carrier that carries of the interior genetic material gene of cell, with biological heredity and variation, close relationship is arranged.Therefore chromosomal increase or minimizing will directly have influence on the expression of biological phenotypic character.Artificially chromosome being doubled, is genetic breeding, and Basic of Biology research provides better technological means.
Haploid breeding is one of breeding technique that plant is commonly used, by certain means, induces the Haploid production plant, then by certain means, chromosome set is doubled, thereby makes plant recovery normal dyeing body number.After the dyed body of haplobiont doubles, in a generation, the dliploid of isozygotying can occur, good the isozygotying of therefrom selecting is that the offspring does not separate, performance neat and consistent, but shortening the breeding cycle.While using haploid breeding, because the haplobiont after doubling can not be subject to covering of dominant gene, the proterties that recessive gene is controlled can be more prone to manifest, and this will be very beneficial to mutation breeding and sudden change genetic research.In addition, polyploid breeding is also one of breeding technique commonly used, and its basic principle is to utilize induced mutations or natural variation etc., by the cell chromosome group, doubles to obtain the polyploid breeding material, meets the improved seeds of people's needs in order to seed selection.Polyploid plant has lot of advantages, such as the infertile property of triploid plant, thereby can reduce plant reproductive growth institute energy requirement, improves output; Also have the polyploidy plant gene dose effect, make polyploid plant there is larger nutrition organs and reproductive organs, also can improve output etc.Therefore, the improvement of chromosome doubling technology will directly bring new hope for plant genetics and breeding.
At present, the most frequently used chromosome doubling method is by colchicine method and low temperature induction method.The mitosis plant cell of processing through colchicine, the generation of its spindle is suppressed, although chromosome divides like this, cell does not separate, therefore, intracellular chromosome number is multiplied.After this, cell is divided and has just been formed polyploid.Low temperature also can make microtubule depolymerization make the chromosome doubling in cell, to colchicine, has similar action principle.But colchicine belongs to carcinogen, the long-term use has threat to operator ' s health; The condition of low temperature induction is groped consuming time, and stable poor, repeatability is bad.And the laughing gas voltolization has and saves time, low toxicity, the advantage of good stability.
The plant chromosome doubling is the process that by biological, physico-chemical method, chromosome is multiplied in its process of growth, and illumination and temperature are the key factors of plant growth.At present, there is no the report of the plant plant laughing gas HIGH PRESSURE TREATMENT container that can meet the illumination demand.
Summary of the invention
The utility model is for above-mentioned deficiency of the prior art, a kind of movable, multi-functional high-pressure bottle of processing for plant plant chromosome doubling has been proposed, it can hold whole plant plant, safety automation realizes that the tank body internal gas pressure is stable, meet plant growth illumination demand, the function of Real-Time Monitoring sample treatment temperature, the easy to operate high-pressure installation of processing for plant plant chromosome doubling, have simultaneously volume little, lightweight, be convenient to the characteristics such as mobile and use.
This programme is achieved by the following technical measures: a kind of high-pressure bottle of processing that doubles for plant chromosome, it comprises cylindrical shell and the end cap up and down be connected with cylindrical shell, it is characterized in that upper end cover is connected by flange seal with cylindrical shell, bottom end cover and barrel soldering are integrated; Be provided with form on cylindrical shell, be provided with temperature-detecting device and pressure-detecting device in the high-pressure bottle bottom, temperature-detecting device and pressure-detecting device are sent temperature signal and pressure signal to the PLC controller, and the PLC controller is controlled the keying of air inlet sequencing valve and exhaust sequencing valve.Lower than when setting value, open the air inlet sequencing valve when the tank body internal pressure, pressure when setting value, is closed the air inlet sequencing valve.When setting, open the exhaust sequencing valve when the tank body internal pressure, pressure when setting value, is closed the exhaust sequencing valve.
In inner barrel, the built-in hand basket of multilayer is set.For the space that takes full advantage of tank body and put just access plant, be provided with the hand basket of handle in tank body.As hand basket is not set, and place only one deck plant in tank body, because plant height differs, if plant height is less, just can not take full advantage of the tank interior space.The basket height can be designed to plurality of specifications, be respectively 1 times, 1/2 times, 1/3 of high-pressure bottle tank body height ... 1/N times, to meet the demand of the different plant height plant of carrying.
Every layer of hand basket comprise the hand basket cylinder and be arranged at the base plate of hand basket cylinder one end, at the other end of hand basket, handle and backing pin is set; Hand basket cylinder and base plate are the orifice-plate type structure, convenient evenly ventilative and eliminating hydrops.
At cylindrical shell upper end cover top, safety valve is set.
Form is set on upper end cover.Form on described high-pressure bottle upper end cover and high-pressure bottle cylindrical shell is the good glass material of light transmission.Form has transmission sunlight and is convenient to observe the function of plant growth condition in tank, if when sunlight illumination is insufficient, and can be in the outer setting illumination lamp source of form.
Described form distributes at cylindrical shell and upper end cover surface uniform.
The high-pressure bottle below arranges bearing, at rest base, wheel is set.Can facilitate the mobile high-voltage device, this travelling wheel has brake function, convenient fixedly high-pressure installation.
Described temperature-detecting device is bimetallic thermometer, thermometer or temperature transmitter with the signal is far passed function.
Described pressure-detecting device is manometer, electro connecting pressure gauge or pressure transmitter with the signal is far passed function.
Air inlet sequencing valve and intake valve are set on bottom end cover, between air inlet sequencing valve and intake valve, check valve are set, between exhaust sequencing valve and upper end cover, air bleeding valve is set, also be provided with blowoff valve on bottom end cover.Realize the characteristics of automatic stabilisation tank body air pressure function and easy to exhaust by sequencing valve, check valve, switch valve combination.Described check valve has the back flow of gas that prevents in the high-pressure bottle function to the source of the gas end.
The beneficial effect of this programme can be learnt according to the narration to such scheme, by adopting above-mentioned structure, the utility model can realize holding whole plant plant, and safety automation realizes that the tank body internal gas pressure is stable, meet plant growth illumination demand, the function of Real-Time Monitoring sample treatment temperature.In addition, the utility model is simple in structure, easy to operate, has very strong practicality.
The accompanying drawing explanation
Fig. 1 is the high-pressure installation structural representation of processing for plant plant chromosome doubling.Fig. 2 is the high-pressure installation vertical view of processing for plant plant chromosome doubling.Fig. 3 is the hand basket figure of handle collapsed state.Fig. 4 is the hand basket figure that handle is mentioned state.
In figure: the 1-cylindrical shell; The 2-flange; The 3-sealing gasket; The 4-securing member; The 5-upper end cover; The 6-handle; The 7-form; The 8-air bleeding valve; 9-exhaust sequencing valve; The 10-safety valve; The 11-temperature-detecting device; 12-air inlet sequencing valve; The 13-intake valve; The 14-check valve; The 15-wheel; The 16-blowoff valve; The 17-bearing; The 18-PLC controller; The 19-pressure-detecting device; The 20-hanger; 21-hand basket cylinder; The 22-handle; The 23-backing pin; 24-hand basket base plate.
Embodiment
Below in conjunction with accompanying drawing, embodiment of the present utility model is described in further detail.
As shown in Figures 1 and 2, a kind of high-pressure bottle of processing that doubles for plant chromosome, it comprises cylindrical shell 1 and the end cap up and down be connected with cylindrical shell 1, and upper end cover 5 and cylindrical shell 1 are tightly connected by flange 2, and bottom end cover and cylindrical shell 1 are welded as a whole; Be provided with form 7 on cylindrical shell 1, be provided with temperature-detecting device 11 and pressure-detecting device 19 in the high-pressure bottle bottom, temperature-detecting device 11 and pressure-detecting device 19 are sent temperature signal and pressure signal to PLC controller 18, and PLC controller 18 is controlled the keying of air inlet sequencing valves 12 and exhaust sequencing valve 9.Lower than when setting value, open air inlet sequencing valve 12 when the high-pressure bottle internal pressure, pressure when setting value, is closed air inlet sequencing valve 12.When setting, open exhaust sequencing valve 9 when the high-pressure bottle internal pressure, pressure when setting value, is closed exhaust sequencing valve 9.
As shown in Figures 3 and 4, for the space that takes full advantage of high-pressure bottle and put just access plant, in cylindrical shell 1 inside, the multilayer hand basket is set.For the space that takes full advantage of tank body and put just access plant, be provided with the hand basket of handle in high-pressure bottle.As hand basket is not set, and place only one deck plant in high-pressure bottle, because plant height differs, if plant height is less, just can not take full advantage of the tank interior space.The hand basket height can be designed to plurality of specifications, be respectively 1 times, 1/2 times, 1/3 of the high-pressure bottle height ... 1/N times, to meet the demand of the different plant height plant of carrying.
Every layer of hand basket comprise hand basket cylinder 21 and be arranged at the base plate of hand basket cylinder 21 1 ends, at the other end of hand basket, handle 22 and backing pin 23 is set; Hand basket cylinder 21 and base plate 24 are the orifice-plate type structure, convenient evenly ventilative and eliminating hydrops.
At cylindrical shell 11 upper end cover 5 tops, safety valve 10 is set.
Described form 7 distributes at cylindrical shell 1 and upper end cover 5 surface uniforms.
The high-pressure bottle below arranges bearing 17, in bearing 17 bottoms, wheel 15 is set.Can facilitate the mobile high-voltage device, this wheel 15 has brake function, convenient fixedly high-pressure bottle.
Described temperature-detecting device 11 is bimetallic thermometer, thermometer or temperature transmitter with the signal is far passed function.
Described pressure-detecting device 19 is manometer, electro connecting pressure gauge or pressure transmitter with the signal is far passed function.
Air inlet sequencing valve 12 and intake valve 13 are set on bottom end cover, between air inlet sequencing valve 12 and intake valve 13, check valve 14 are set, between exhaust sequencing valve 9 and upper end cover 5, air bleeding valve 8 is set, blowoff valve 16 is set on bottom end cover.Realize the characteristics of automatic stabilisation tank body air pressure function and easy to exhaust by sequencing valve, check valve, switch valve combination.Described check valve 14 has the back flow of gas that prevents in the high-pressure bottle function to the source of the gas end.
For not affecting plant in the access tank body, temperature-detecting device 11 and pressure-detecting device 19 are arranged at the bottom of high-pressure bottle tank body.
When the utility model is used, pending plant is put into to hand basket, hand basket is put into to high-pressure bottle cylindrical shell 1, cover upper end cover 2, close air bleeding valve 8.Set operating pressure, open air inlet switch 13.Processing is closed air inlet switch 13 after finishing, and opens air bleeding valve 8, while waiting inside and outside pressure of high-pressure bottle cylindrical shell 1 identical, opens high-pressure bottle upper end cover 2, takes out hand basket and processes plant.In the plant processing procedure, while needing monitoring high-pressure bottle cylindrical shell 1 interior temperature, by bimetallic thermometer, show, read real time temperature.
Claims (10)
1. a high-pressure bottle that doubles to process for plant chromosome, it comprises cylindrical shell and the end cap up and down be connected with cylindrical shell, it is characterized in that upper end cover is connected by flange seal with cylindrical shell, bottom end cover and barrel soldering are integrated; Be provided with form on cylindrical shell, be provided with temperature-detecting device and pressure-detecting device in the high-pressure bottle bottom, temperature-detecting device and pressure-detecting device are sent temperature signal and pressure signal to the PLC controller, and the PLC controller is controlled the keying of air inlet sequencing valve and exhaust sequencing valve.
2. the high-pressure bottle of processing that doubles for plant chromosome according to claim 1, is characterized in that, in inner barrel, the built-in hand basket of multilayer is set.
3. the high-pressure bottle of processing that doubles for plant chromosome according to claim 2, is characterized in that every layer of hand basket comprises the hand basket cylinder and be arranged at the base plate of hand basket cylinder one end, at the other end of hand basket, handle and backing pin is set; Hand basket cylinder and base plate are the orifice-plate type structure.
4. the high-pressure bottle of processing that doubles for plant chromosome according to claim 1, is characterized in that, at the upper end cover top, safety valve is set.
5. the high-pressure bottle of processing that doubles for plant chromosome according to claim 1, is characterized in that arranging glass vision panel on upper end cover.
6. the high-pressure bottle of processing that doubles for plant chromosome according to claim 5, is characterized in that described form distributes at cylindrical shell and upper end cover surface uniform.
7. the high-pressure bottle of processing that doubles for plant chromosome according to claim 1, is characterized in that the high-pressure bottle below arranges bearing, arranges wheel at rest base.
8. the high-pressure bottle of processing that doubles for plant chromosome according to claim 1, is characterized in that described temperature-detecting device is bimetallic thermometer, thermometer or temperature transmitter with the signal is far passed function.
9. the high-pressure bottle of processing that doubles for plant chromosome according to claim 1, is characterized in that described pressure-detecting device is manometer, electro connecting pressure gauge or pressure transmitter with the signal is far passed function.
10. the high-pressure bottle of processing that doubles for plant chromosome according to claim 1, it is characterized in that arranging air inlet sequencing valve and intake valve on bottom end cover, between air inlet sequencing valve and intake valve, check valve is set, between exhaust sequencing valve and upper end cover, air bleeding valve is set, also is provided with blowoff valve on bottom end cover.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320389239 CN203353360U (en) | 2013-07-02 | 2013-07-02 | High-pressure vessel for plant seedling chromosome reduplication treatment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320389239 CN203353360U (en) | 2013-07-02 | 2013-07-02 | High-pressure vessel for plant seedling chromosome reduplication treatment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203353360U true CN203353360U (en) | 2013-12-25 |
Family
ID=49801207
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201320389239 Expired - Fee Related CN203353360U (en) | 2013-07-02 | 2013-07-02 | High-pressure vessel for plant seedling chromosome reduplication treatment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203353360U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113287513A (en) * | 2021-07-01 | 2021-08-24 | 金陵科技学院 | Eggplant haploid plant sexual propagation doubling method based on improvement of pollen activity |
-
2013
- 2013-07-02 CN CN 201320389239 patent/CN203353360U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113287513A (en) * | 2021-07-01 | 2021-08-24 | 金陵科技学院 | Eggplant haploid plant sexual propagation doubling method based on improvement of pollen activity |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101390477A (en) | Integration device for edible fungus culture medium sterilization, inoculation, cultivation, fruiting | |
| CN103242080B (en) | Mushroom liquid strain as well as preparation method, preparation device and application thereof | |
| CN209338554U (en) | A kind of Microorganism in Water detection device | |
| CN203353360U (en) | High-pressure vessel for plant seedling chromosome reduplication treatment | |
| CN210247724U (en) | Seedling growth index measuring device for tomato plug seedling | |
| CN204994408U (en) | Quantitative cup | |
| CN201280554Y (en) | Miniature multi-use fungus liquid fermentation tank | |
| CN203602623U (en) | Microorganism high-pressure incubator | |
| CN206866150U (en) | Aseptic seedlings of plants culture apparatus is used in a kind of endophyte tieback experiment | |
| CN201605272U (en) | Photosynthetic bacterium cultivating pot | |
| CN202175671U (en) | Humidity-adjustable microorganism incubator | |
| CN205223234U (en) | Fermentation tank | |
| CN201301313Y (en) | Photosynthetic bacteria culturing device | |
| RU155563U1 (en) | Tray plant for growing microalgae | |
| CN103125269A (en) | Simple fermentation device for liquid spawn submerged cultivation | |
| CN202465663U (en) | Continuous culture device for unicellular algae | |
| CN202857566U (en) | Composite type sea cucumber cultivating device utilizing solar heating | |
| CN106010939A (en) | Alga culture device | |
| CN209669223U (en) | A kind of fermentor | |
| CN208500918U (en) | The algae culturing system of hanging bag and the application hanging bag | |
| CN202663839U (en) | Novel edible fungi liquid ferment pot | |
| CN205152231U (en) | Continuous culture device of lay mattress clothing algae | |
| CN218680702U (en) | Edible mushroom cross breeding culture utensil | |
| CN208754907U (en) | A kind of constant temperature incubation test bottle for tealeaves tissue cultures | |
| CN104099246A (en) | Minitype incubator |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20131225 Termination date: 20160702 |