CN203324280U - Reagent box for rapidly detecting neuron-specific enolase - Google Patents
Reagent box for rapidly detecting neuron-specific enolase Download PDFInfo
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- CN203324280U CN203324280U CN2013203545468U CN201320354546U CN203324280U CN 203324280 U CN203324280 U CN 203324280U CN 2013203545468 U CN2013203545468 U CN 2013203545468U CN 201320354546 U CN201320354546 U CN 201320354546U CN 203324280 U CN203324280 U CN 203324280U
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Abstract
The utility model discloses a reagent box for rapidly detecting a neuron-specific enolase. The reagent box comprises a box body and a box cover connected with the box body, wherein the box cover is provided with an electron alarm clock; the box body is internally provided with a sponge support the size of which is as the same as that of the box body, the sponge support is provided with a magnetic separation reagent bottom groove, a tracing reactant reagent bottle groove, a stable enhancer reagent bottle groove, a chemiluminiscence substrate reagent bottle groove, a calibration reagent bottle groove, a quality control substance-standard substance reagent bottle groove, a buffer solution reagent bottle groove and a cleaning solution reagent bottle groove, and corresponding reagent bottles are placed in the grooves. The reagent box provided by the utility model has the advantages that the detection sensitivity is high, the peculiar property is good, the variation is small, the detection result is reliable, and the reagent box provides specific, rapid and reliable basis for clinical diagnosis.
Description
Technical field
The utility model relates to immunoassay and biomedical sector, is specially a kind of fast detecting nerve specificity olefinic alcohol enzyme kit.
Background technology
Neuronspecific enolase (NSE) is to participate in a kind of in the enolase of glycolytic pathway, is present in nerve fiber and neuroendocrine tissue.NSE is the highest in the activity of brain tissue cell, and the activity level of peripheral nerve and neurosecretion tissue is placed in the middle, and minimum sees non-nervous tissue, serum and spinal fluid.Containing after the cytoclasis of NSE, can make NSE be released into serum, therefore can detect, normal human serum NSE level is 5～15ng/ml.The result of study of immunocytochemistry discloses, the NSE high specific be positioned neuron and neuroendocrine cell, as APUD cell system.By monitoring, because of activity of gene expression, change and cell phenotype change due to the biological fluid of Disease of metabolic disorder, as the NSE activity level in serum and spinal cord, can provide to disease the useful information of diagnosis.
The research of labelled immune analytical technology and application development are rapid nearly ten years, have been widely used in each field of biomedical fundamental research and clinical disease diagnosis.Wherein the technical matters maturation, have advance and practical, and what be easy to promote mainly contains: four kinds of radiommunoassay, EIA enzyme immunoassay, time resolved fluoro-immunoassay and chemiluminescence immune assays etc.The basic theories of these ultramicron detection techniques is substantially identical, but tracer agent used and the signal that sends are different.According to a large amount of experimental results and clinical practice data, from practicality, stability, accuracy and development prospect, be followed successively by: chemiluminescence immune assay, time resolved fluoro-immunoassay, radiommunoassay and EIA enzyme immunoassay.There is shortcomings in radio immunoassay, as complicated operation, measurement result is unstable, the reagent holding time is short, radioactive contamination, instrument costliness etc.Enzyme immunoassay (EIA) sensitivity is low, and influence factor is more, easily causes false negative and false positive.Chemiluminescence immune assay quantification kit of the prior art is closed full automatic chemiluminescence measuring system, needs expensive Full-automatic chemiluminescence measuring instrument, thereby limited, promotes the use of, and can't be widely used in clinical diagnosis and research work.
At home, neuronspecific enolase detects and mainly with external import reagent and SABC reagent, is applied as the master clinically, and external import reagent price is very expensive, to the patient, brings very large financial burden, is unfavorable in basic unit universal.Domestic neuronspecific enolase (NSE) immunochemiluminescence detection kit with independent intellectual property right is at present also less, also only rests on 96 hole micro-pore plate type technical merits.This sensitivity is lower, linearity is narrow, specificity is poor, also is unfavorable for high-throughout full-automatic detection.In addition, due to the characteristic of board-like detection reagent, the phase of relatively imitating is shorter, adds the easy inactivation of neuronspecific enolase (NSE) antigen, and the upper reliability of actual use is lower.
The utility model content
The technical matters that the utility model solves is to provide a kind of fast detecting nerve specificity olefinic alcohol enzyme kit, to solve the problem proposed in the above-mentioned background technology.
The technical matters that the utility model solves realizes by the following technical solutions:
Fast detecting nerve specificity olefinic alcohol enzyme kit, comprise box body and the lid be connected with box body, lid is provided with electronic alarm clock, be placed with the sponge holder identical with the box body size in box body, described sponge holder is provided with magnetic separation agent reagent bottle groove, spike reactant reagent bottle groove, stabilizing reinforcer reagent bottle groove, chemical luminous substrate reagent bottle groove, calibration object reagent bottle groove, quality-control product-standard items reagent bottle groove, damping fluid reagent bottle groove and cleaning fluid reagent bottle groove, and above-mentioned groove is put into corresponding reagent bottle.
Further, described magnetic separation agent comprises labeled neurons specificity olefinic alcohol enzyme capture antibody and makes biotinylated antibody and Streptavidin magnetic particle.
Further, described spike reactant comprises the neuronspecific enolase tracer antibody of acridinium ester mark.
Further, described stabilizing reinforcer comprises the polycomponent immune complex that anti-heterophile antibody disturbs.
Beneficial effect:
The utility model detection sensitivity is high, and specific performance is good, it is little to make a variation, and testing result is reliable; Can be clinical diagnosis more special, quick, reliable foundation is provided.
The accompanying drawing explanation
Fig. 1 is structural representation of the present utility model.
Embodiment
In order to make of the present utility modelly to realize technological means, creation characteristic, reach purpose and effect is easy to understand, below in conjunction with concrete diagram, further set forth the utility model.
As shown in Figure 1, fast detecting nerve specificity olefinic alcohol enzyme kit, comprise box body 1 and the lid 2 be connected with box body 1, lid 2 is provided with electronic alarm clock 3, be placed with the sponge holder 4 identical with the box body size in box body 1, described sponge holder 4 is provided with magnetic separation agent reagent bottle groove 5, spike reactant reagent bottle groove 6, stabilizing reinforcer reagent bottle groove 7, chemical luminous substrate reagent bottle groove 8, calibration object reagent bottle groove 9, quality-control product-standard items reagent bottle groove 10, damping fluid reagent bottle groove 11 and cleaning fluid reagent bottle groove 12, above-mentioned groove is put into corresponding reagent bottle.
In the utility model, described magnetic separation agent comprises labeled neurons specificity olefinic alcohol enzyme capture antibody and makes biotinylated antibody and Streptavidin magnetic particle, described spike reactant comprises the neuronspecific enolase tracer antibody of acridinium ester mark, and described stabilizing reinforcer comprises the polycomponent immune complex that anti-heterophile antibody disturbs.
The utility model adopts sample and reagent is once added, and facilitates manual operation also to be beneficial to neuronspecific enolase (NSE) the magnetic microparticle separating chemiluminescence immunoassays that improve full automatic working efficiency; Each reagent component comprises standard items-quality-control product, magnetic separation agent, chemical luminous substrate etc., has good stability, and the term of validity can be to more than 1 year, and detection sensitivity is high, and specific performance is good, it is little to make a variation.
Above demonstration and described ultimate principle of the present utility model and principal character and advantage of the present utility model.The technician of the industry should understand; the utility model is not restricted to the described embodiments; that in above-described embodiment and instructions, describes just illustrates principle of the present utility model; under the prerequisite that does not break away from the utility model spirit and scope; the utility model also has various changes and modifications, and these changes and improvements all fall in claimed the utility model scope.Claimed scope of the present utility model is defined by appending claims and equivalent thereof.
Claims (4)
1. fast detecting nerve specificity olefinic alcohol enzyme kit, comprise box body and the lid be connected with box body, it is characterized in that: lid is provided with electronic alarm clock, be placed with the sponge holder identical with the box body size in box body, described sponge holder is provided with magnetic separation agent reagent bottle groove, spike reactant reagent bottle groove, stabilizing reinforcer reagent bottle groove, chemical luminous substrate reagent bottle groove, calibration object reagent bottle groove, quality-control product one standard items reagent bottle groove, damping fluid reagent bottle groove and cleaning fluid reagent bottle groove, above-mentioned groove is put into corresponding reagent bottle.
2. fast detecting nerve specificity olefinic alcohol enzyme kit according to claim 1, it is characterized in that: described magnetic separation agent comprises labeled neurons specificity olefinic alcohol enzyme capture antibody and makes biotinylated antibody and Streptavidin magnetic particle.
3. fast detecting nerve specificity olefinic alcohol enzyme kit according to claim 1, it is characterized in that: described spike reactant comprises the neuronspecific enolase tracer antibody of acridinium ester mark.
4. fast detecting nerve specificity olefinic alcohol enzyme kit according to claim 1 is characterized in that: described stabilizing reinforcer comprises the polycomponent immune complex that anti-heterophile antibody disturbs.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013203545468U CN203324280U (en) | 2013-06-20 | 2013-06-20 | Reagent box for rapidly detecting neuron-specific enolase |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013203545468U CN203324280U (en) | 2013-06-20 | 2013-06-20 | Reagent box for rapidly detecting neuron-specific enolase |
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| CN203324280U true CN203324280U (en) | 2013-12-04 |
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| CN2013203545468U Expired - Lifetime CN203324280U (en) | 2013-06-20 | 2013-06-20 | Reagent box for rapidly detecting neuron-specific enolase |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105785002A (en) * | 2016-04-14 | 2016-07-20 | 深圳市疾病预防控制中心 | Paralytic shellfish toxin cell detection method based on alpha-enolase |
| CN109187973A (en) * | 2018-08-17 | 2019-01-11 | 迪瑞医疗科技股份有限公司 | For detecting the chemiluminescence immunoassay kit of neuronspecific enolase |
| CN109187971A (en) * | 2018-08-17 | 2019-01-11 | 迪瑞医疗科技股份有限公司 | Neuronspecific enolase chemiluminescence immune detection reagent kit and preparation method thereof |
-
2013
- 2013-06-20 CN CN2013203545468U patent/CN203324280U/en not_active Expired - Lifetime
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105785002A (en) * | 2016-04-14 | 2016-07-20 | 深圳市疾病预防控制中心 | Paralytic shellfish toxin cell detection method based on alpha-enolase |
| CN105785002B (en) * | 2016-04-14 | 2017-05-24 | 深圳市疾病预防控制中心 | Paralytic shellfish toxin cell detection method based on alpha-enolase |
| CN109187973A (en) * | 2018-08-17 | 2019-01-11 | 迪瑞医疗科技股份有限公司 | For detecting the chemiluminescence immunoassay kit of neuronspecific enolase |
| CN109187971A (en) * | 2018-08-17 | 2019-01-11 | 迪瑞医疗科技股份有限公司 | Neuronspecific enolase chemiluminescence immune detection reagent kit and preparation method thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20160107 Address after: 215163 building, No. 8, Jinfeng Road, hi tech Zone, Suzhou, Jiangsu, China Patentee after: SUZHOU HYBIOME BIOMEDICAL ENGINEERING Co.,Ltd. Address before: 215163 building, No. 8, Jinfeng Road, hi tech Zone, Suzhou, Jiangsu, China Patentee before: SUZHOU HYBIOME BIOREAGENT Co.,Ltd. |
|
| CX01 | Expiry of patent term |
Granted publication date: 20131204 |
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| CX01 | Expiry of patent term |