CN105004781A - Dopamine detecting method based on paper-base electrochemistry device - Google Patents
Dopamine detecting method based on paper-base electrochemistry device Download PDFInfo
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Abstract
The invention discloses a dopamine detecting method based on a paper-base electrochemical device. The dopamine detecting method includes the following steps: 1, filter paper serves as a detecting platform for a paper-base substrate, and an electrode is manually drawn through a pencil, wherein graphite serves as materials of the electrode, a circular hydrophilic region is arranged in the middle of the detecting platform, and a hydrophobic region is arranged on the periphery of the circular hydrophilic region; 2, dopamine is detected with a differential pulse voltammetry method, and a working curve for measuring the dopamine is made, wherein the detecting platform can sensitively detect the dopamine when interference objects exist; the dopamine in a practical sample can be detected when the to-be-detected sample is added to the detecting platform. According to the dopamine detecting method based on the paper-base electrochemical device, a paper-base electrochemical analysis system is a novel electrochemical device, is easy to manufacture and low in cost and can be used for various application fields including clinic diagnosis portable and disposable analysis devices, food quality control, environment monitoring and the like.
Description
Technical field
The present invention relates to field of biological technology detection, be specifically related to a kind of method of the mensuration dopamine based on paper substrate electrochemical appliance.
Background technology
Dopamine (DA), chemical name is 4-(2-ethylamino-) benzene-1,2-diphenol, is a kind of key neurotransmitter in hypothalamus and pituitary gland, is extensively present in vertebra and spinal animal; Lack dopamine can allow people feel slack weak, feel dizzy.Excessive dopamine, then can produce diametrically opposite problem, and as caused people's irritability, uncontrollable mood, neural reflex is accelerated.Case shows as extremely excited, and long flood tide electric pulse stimulation can cause irreversible damage to nerve.The exception of DOPAMINE CONTENT IN RABBIT can cause the numerous disease such as parkinsonism and schizophrenia.Therefore, the detection of dopamine is for quick diagnosis relevant disease clinically, and there is very important meaning the aspects such as the quality control of neuro-physiology research and related drugs.The method of conventional detection dopamine has High Performance Liquid Chromatography with Fluorescence Detection, capillary electrophoresis, chemoluminescence method etc., and these traditional methods have mensuration accurately, the advantage that selectivity is good.But these methods shortcomings such as also ubiquity instrument price is expensive, operating cost is high, not portable, are very limited in monitoring and on-site measurement etc. continuously, not reach in sample analysis fast, Multi-example, easy, real-time analysis requirement.Therefore the interest that a kind of simple, accurate, quick, sensitive, easy-operating dopamine detection method causes many researchers is designed.
Summary of the invention
Object of the present invention provides a kind of method of the mensuration dopamine based on paper substrate electrochemical appliance, solves one or more in above-mentioned prior art problem.
According to an aspect of the present invention, a kind of method of the mensuration dopamine based on paper substrate electrochemical appliance, comprises the steps:
(1) detection platform using filter paper as paper substrate substrate, pencil manual drawing electrode, using graphite as electrode material, having circular hydrophilic region in the middle of detection platform, is around hydrophobic region;
(2) Differential Pulse Voltammetry measures dopamine, makes and measures the working curve of dopamine, detection platform can under chaff interference exists sensitive determination dopamine; Testing sample is added the detection that detection platform carries out dopamine in actual sample.
The method of a kind of mensuration dopamine based on paper substrate electrochemical appliance provided by the invention, paper substrate electrochemical analysis system is a kind of new electrochemical appliance, it makes simple, cost is low, can be used for many applications, comprise portable, the disposable analytical equipment of clinical diagnosis, food quality control and environmental monitoring etc.
In some embodiments, the detection platform of the paper substrate substrate in step (1) and the method for making of electrode template as follows,
(1) utilize Photoshop software by drawing, the pattern of figure is: the length of side is the square of 19.5mm, the circle of in the middle of square to be diameter be 6.5mm, and border circular areas be white, and remainder is black;
(2) be printed on filter paper by drawn graphics template, then filter paper be placed in baking oven, the temperature of baking oven is set to 150 degrees Celsius, and the time is 3 hours; Its objective is and the wax of black region is penetrated into filter paper middle layer, form hydrophobic region, filter paper white portion is hydrophilic area; Then filter paper is dried, template is cut.
(3) with 6B pencil for electrode material, the detection platform of the paper substrate substrate made draws out three-electrode system, three electrode dimensional requirement is: the wide of working electrode is 3mm, long is 6mm, length in hydrophilic area is 2mm, be 2mm to electrode and the wide of contrast electrode, long is 6mm, and the length in hydrophilic area is 2mm.
In some embodiments, the detection platform of the paper substrate substrate designed is connected with CHI1230B electrochemical workstation, then prepare the dopamine standard solution that 0.1,0.5,1,5,10,50,100,400,700 micromoles often rise concentration, the phosphate buffer of pH=7.4 is as electrolyte solution; Get the midparent aqua region that 10 microlitre dopamine standard solution are added drop-wise to electrode, differential pulse voltammetry voltammetric scan is made in the potential range of-0.2 ~ 0.6V, obtain the scanning curve that 0.1,0.5,1,5,10,50,100,400,700 micromoles often rise the dopamine solution of concentration, the peak point current corresponding with it according to the concentration of standard solution, the linear relationship of the two can be calculated, obtain working curve; When detecting the dopamine in testing sample urine sample or blood sample, the concentration of dopamine in sample can be obtained according to the current value of detection curve.
Graphite is a kind of extraordinary conductive material, and the more some other conventional conductive material of its price is cheap, and stability is also better than some other conventional electrode materials.Therefore graphite is combined with paper, builds Disposable, paper-based electrochemical analysis device and there is the advantage such as low cost, portability; And highly developed infrastructure printing industry, make the production economy of this paper substrate analytical equipment have again developability; Take paper as research substrate, by manual drawing pencil electrode, build three-electrode system, makes a complete electrochemical analysis pick-up unit, this graphite paper based electrochemical system makes simply, and low price is easy to operate, good stability.
Graphite paper based electrochemical devices has the advantage of following uniqueness: 1. the price of paper and graphite all relative moderate, are easy to get.2. the filter paper of porous structure is formed primarily of cellulose, can supporting electrolyte, and the sample size of needs is few, only counts microlitre.3. paper is a kind of well filtrator.4. graphite electrode has metastable electrochemical properties.
As disposable electrode, it can provide clean surface when each mensuration, and in addition, cheap graphite electrode, avoids expensive instrument and maintenance cost, and meanwhile, its making is simple, can produce in large quantity.
The paper substrate electrochemical sensing device that the present invention builds, not only avoid ascorbic acid, uric acid to the interference of dopamine determination, and improve sensitivity and the reappearance of detection, compared with existing electrochemical sensor, it is wide that the present invention has the range of linearity, and detectability is low, make advantage simple, with low cost, analyze and detect rapidly, convenient, may be used for clinical analysis.
Electrochemical method obtains the very big concern of people due to the traditional advantage in high sensitivity, short measuring period, high reliability etc., the analysis being widely used in neurotransmitter detects.Electricity analytical method is usually pre-concentration means with electro-deposition, and the pre-concentration before mensuration or enrichment will improve sensitivity and the selectivity of analysis greatly, thus are more suitable for the mensuration to trace materials.Relative to other detection method, electrochemical method instrument and equipment is simple, is easy to carry, easy to operate, can detect many kinds of substance fast simultaneously, is easy to realize scene and automatically detects, obtained and promote more widely in contemporary health analysis.
In the development of analytical chemistry, the research of patterning filter paper is that the research developing portable analysers provides more wide thinking.Filter paper has the features such as absorbability is good, soft easy to operate, portable, with low cost, it is a kind of desirable material for creation analysis system, paper is formed primarily of cellulose, there is porous, hydrophilic performance, on its surface containing the hydrophilic radical such as a large amount of carboxyls and hydroxyl, the moisture more much bigger than own wt can be adsorbed.In addition, it is low that paper also has cost, and quality is light, and the advantage such as be easy to carry, and compared with the substrate plate such as polymkeric substance, glass, its maximum advantage can be biodegradable after using, environmental sound.And graphite is a kind of extraordinary conductive material, and the more some other conventional conductive material of its price is cheap, and stability is also better than some other conventional electrode materials.Therefore graphite is combined with paper, builds disposable graphite paper base electrode detection analytical equipment and there is the advantage such as low cost, portability.
Differential Pulse Voltammetry is a kind of electrochemical measurement means, is the deriving method of linear sweep voltammetry and ladder water price, namely on its basis, adds certain potential pulse.At the pre-test electric current of electromotive force change, reduce the impact of charging current in this way.
Accompanying drawing explanation
Fig. 1 is the electrode model figure of the graphite paper substrate substrate of the method for a kind of mensuration dopamine based on paper substrate electrochemical appliance of the present invention.
Embodiment
Based on a method for the mensuration dopamine of paper substrate electrochemical appliance, comprise the steps:
(1) detection platform using filter paper as paper substrate substrate, pencil manual drawing electrode, using graphite as electrode material, having circular hydrophilic region in the middle of detection platform, is around hydrophobic region.
(2) Differential Pulse Voltammetry measures dopamine, makes and measures the working curve of dopamine, detection platform can under chaff interference exists sensitive determination dopamine; Testing sample is added the detection that detection platform carries out dopamine in actual sample.
As shown in Figure 1, the detection platform of the paper substrate substrate in step (1) and the method for making of electrode template as follows,
(1) utilize Photoshop software by drawing, the pattern of figure is: the length of side is the square of 19.5mm, the circle of in the middle of square to be diameter be 6.5mm, and border circular areas be white, and remainder is black;
(2) be printed on filter paper by drawn graphics template, then filter paper be placed in baking oven, the temperature of baking oven is set to 150 degrees Celsius, and the time is 3 hours; Its objective is and the wax of black region is penetrated into filter paper middle layer, form hydrophobic region, filter paper white portion is hydrophilic area; Then filter paper is dried, template is cut.
(3) with 6B pencil for electrode material, the detection platform of the paper substrate substrate made draws out three-electrode system, three electrode dimensional requirement is: the wide of working electrode is 3mm, long is 6mm, length in hydrophilic area is 2mm, be 2mm to electrode and the wide of contrast electrode, long is 6mm, and the length in hydrophilic area is 2mm.
In some embodiments, the detection platform of the paper substrate substrate designed is connected with CHI1230B electrochemical workstation, then prepare the dopamine standard solution that 0.1,0.5,1,5,10,50,100,400,700 micromoles often rise concentration, the phosphate buffer of pH=7.4 is as electrolyte solution; Get the midparent aqua region that 10 microlitre dopamine standard solution are added drop-wise to electrode, differential pulse voltammetry voltammetric scan is made in the potential range of-0.2 ~ 0.6V, obtain the scanning curve that 0.1,0.5,1,5,10,50,100,400,700 micromoles often rise the dopamine solution of concentration, the peak point current corresponding with it according to the concentration of standard solution, the linear relationship of the two can be calculated, obtain working curve; When detecting the dopamine in testing sample urine sample or blood sample, the concentration of dopamine in sample can be obtained according to the current value of detection curve.
Graphite is a kind of extraordinary conductive material, and the more some other conventional conductive material of its price is cheap, and stability is also better than some other conventional electrode materials.Therefore graphite is combined with paper, builds Disposable, paper-based electrochemical analysis device and there is the advantage such as low cost, portability; And highly developed infrastructure printing industry, make the production economy of this paper substrate analytical equipment have again developability; Take paper as research substrate, by manual drawing pencil electrode, build three-electrode system, makes a complete electrochemical analysis pick-up unit, this graphite paper based electrochemical system makes simply, and low price is easy to operate, good stability.
Graphite paper based electrochemical devices has the advantage of following uniqueness: 1. the price of paper and graphite all relative moderate, are easy to get.2. the filter paper of porous structure is formed primarily of cellulose, can supporting electrolyte, and the sample size of needs is few, only counts microlitre.3. paper is a kind of well filtrator.4. graphite electrode has metastable electrochemical properties.
As disposable electrode, it can provide clean surface when each mensuration, and in addition, cheap graphite electrode, avoids expensive instrument and maintenance cost, and meanwhile, its making is simple, can produce in large quantity.
The paper substrate electrochemical sensing device that the present invention builds, not only avoid ascorbic acid, uric acid to the interference of dopamine determination, and improve sensitivity and the reappearance of detection, compared with existing electrochemical sensor, it is wide that the present invention has the range of linearity, and detectability is low, make advantage simple, with low cost, analyze and detect rapidly, convenient, may be used for clinical analysis.
The method of a kind of mensuration dopamine based on paper substrate electrochemical appliance provided by the invention, paper substrate electrochemical analysis system is a kind of new electrochemical appliance, it makes simple, cost is low, can be used for many applications, comprise portable, the disposable analytical equipment of clinical diagnosis, food quality control and environmental monitoring etc.
The above is only optimal way of the present invention; it should be pointed out that to those skilled in the art, without departing from the concept of the premise of the invention; can also make some similar distortion and improvement, these also should be considered as within protection scope of the present invention.
Claims (3)
1., based on a method for the mensuration dopamine of paper substrate electrochemical appliance, it is characterized in that being, comprise the steps:
(1) detection platform using filter paper as paper substrate substrate, pencil manual drawing electrode, using graphite as electrode material, having circular hydrophilic region in the middle of detection platform, is around hydrophobic region;
(2) Differential Pulse Voltammetry measures dopamine, makes and measures the working curve of dopamine, detection platform can under chaff interference exists sensitive determination dopamine; Testing sample is added the detection that detection platform carries out dopamine in actual sample.
2. the method for a kind of mensuration dopamine based on paper substrate electrochemical appliance according to claim 1, is characterized in that being, the detection platform of the paper substrate substrate in described step (1) and the method for making of electrode template as follows,
(1) utilize Photoshop software by drawing, the pattern of figure is: the length of side is the square of 19.5mm, the circle of in the middle of square to be diameter be 6.5mm, and border circular areas be white, and remainder is black;
(2) be printed on filter paper by drawn graphics template, then filter paper be placed in baking oven, the temperature of baking oven is set to 150 degrees Celsius, and the time is 3 hours; Its objective is and the wax of black region is penetrated into filter paper middle layer, form hydrophobic region, filter paper white portion is hydrophilic area; Then filter paper is dried, template is cut.
(3) with 6B pencil for electrode material, the detection platform of the paper substrate substrate made draws out three-electrode system, three electrode dimensional requirement is: the wide of working electrode is 3mm, long is 6mm, length in hydrophilic area is 2mm, be 2mm to electrode and the wide of contrast electrode, long is 6mm, and the length in hydrophilic area is 2mm (as Fig. 1).
3. the method for a kind of mensuration dopamine based on paper substrate electrochemical appliance according to claim 2, it is characterized in that, the detection platform of the paper substrate substrate designed is connected with CHI1230B electrochemical workstation, then prepare the dopamine standard solution that 0.1,0.5,1,5,10,50,100,400,700 micromoles often rise concentration, the phosphate buffer of pH=7.4 is as electrolyte solution; Get the midparent aqua region that 10 microlitre dopamine standard solution are added drop-wise to electrode, differential pulse voltammetry voltammetric scan is made in the potential range of-0.2 ~ 0.6V, obtain the scanning curve that 0.1,0.5,1,5,10,50,100,400,700 micromoles often rise the dopamine solution of concentration, the peak point current corresponding with it according to the concentration of standard solution, the linear relationship of the two can be calculated, obtain working curve; When detecting the dopamine in testing sample urine sample or blood sample, the concentration of dopamine in sample can be obtained according to the current value of detection curve.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105606682A (en) * | 2016-01-26 | 2016-05-25 | 南通大学 | Method for determining glucose based on paper-based electrochemical device |
| CN105911116A (en) * | 2016-05-16 | 2016-08-31 | 苏州感测通信息科技有限公司 | Paper chip for rapidly detecting organophosphorus pesticide and production method thereof |
| CN106645320A (en) * | 2016-11-15 | 2017-05-10 | 中国科学院大学 | Flexible electrode based on biocompatible materials, preparation method of flexible electrode and application |
| CN110082407A (en) * | 2019-03-29 | 2019-08-02 | 华东师范大学 | A kind of flexibility gold electrode and preparation method |
| CN114376578A (en) * | 2022-01-07 | 2022-04-22 | 河北工业大学 | Super-hydrophobic paper-based graphite electrocardioelectrode slice and preparation method and use method thereof |
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Patent Citations (2)
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| CN101726531A (en) * | 2009-12-16 | 2010-06-09 | 厦门大学 | Electrochemical detection method of dopamine in body fluid |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105606682A (en) * | 2016-01-26 | 2016-05-25 | 南通大学 | Method for determining glucose based on paper-based electrochemical device |
| CN105911116A (en) * | 2016-05-16 | 2016-08-31 | 苏州感测通信息科技有限公司 | Paper chip for rapidly detecting organophosphorus pesticide and production method thereof |
| CN106645320A (en) * | 2016-11-15 | 2017-05-10 | 中国科学院大学 | Flexible electrode based on biocompatible materials, preparation method of flexible electrode and application |
| CN110082407A (en) * | 2019-03-29 | 2019-08-02 | 华东师范大学 | A kind of flexibility gold electrode and preparation method |
| CN114376578A (en) * | 2022-01-07 | 2022-04-22 | 河北工业大学 | Super-hydrophobic paper-based graphite electrocardioelectrode slice and preparation method and use method thereof |
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