CN203275412U - Kit for chemical luminescence immunoassay of CA242 (tumor marker) - Google Patents
Kit for chemical luminescence immunoassay of CA242 (tumor marker) Download PDFInfo
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- CN203275412U CN203275412U CN 201320316308 CN201320316308U CN203275412U CN 203275412 U CN203275412 U CN 203275412U CN 201320316308 CN201320316308 CN 201320316308 CN 201320316308 U CN201320316308 U CN 201320316308U CN 203275412 U CN203275412 U CN 203275412U
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Abstract
The utility model relates to a kit for the chemical luminescence immunoassay of a CA242 (tumor marker). The kit comprises a kit body and a kit cover; the kit cover is connected with the kit body; a square disc which is arranged in the kit body is connected with the kit cover; a first sponge tray which is similar to the kit body in size is arranged in the kit body, and a second sponge tray which is similar to the square disc in size is arranged in the square disc; the first sponge tray is provided with seven first grooves; and an anti-CA242 clonal antibody labeled magnetic separation reagent bottle, an acridinium-ester labeled CA242 monoclonal antibody reagent bottle, a reaction-enhancer bottle, a diluent bottle, a calibration reagent and quality-control reagent bottle, a concentrated cleaning solution bottle and an acridinium-ester acted alkaline solution bottle are arranged in the first grooves respectively. The kit provided by the utility model has high immunoassay sensitivity and specificity. By adopting the kit, excellent performance parameters can be achieved, stable and reliable reaction process and sensitive and effective experimental data can be guaranteed, the performance of the product can be improved, and the cost of the product can be reduced greatly.
Description
Technical field
The utility model relates to the immune detection analysis field, is specially a kind of chemiluminescence immunoassay and detects the CA242 kit.
Background technology
CA242 is a kind of novel tumor markers, it is a kind of mucin sugar antigen, found by people such as Lindholm in 1985, it is that the people ties-a kind of antigen that monoclonal antibody C242 that rectum cancer cell strain Colo205 obtains through the hybridoma technology immune mouse can identify, and its antigenic determinant is a kind of sialylated sugar newly.Immunochemistry studies have shown that, CA242 is different from existing Tumor-assaciated mucin antigen, as CA19-9, and CA50 etc.The expression of CA242 antigenic determinant as Staphylococal Protein A (for example CA19-9) Sialy Lewis (SLea) is thought in sialylated Louis, is on mucin, yet in optimum and malignant tumour, some difference that the expression of CA242 and SLea is relative.Compare with SLea in malignant tumour, the expression of CA242 antigenic determinant has more specificity; In optimum hepatobiliary and pancreatic diseases, its high specific is particularly noticeable.Therefore the mensuration of CA242 antigenic determinant is compared with the SLea that contains mucin antigen and is just had higher specificity and diagnostic significance.
Many clinical research confirmations, CA242 detect has the sensitivity higher than other tumor markerses: to the diagnosis of cancer of pancreas, CA242 is better than CA19-9, and susceptibility can reach 66%～100%, and the susceptibility of colorectal cancer is also reached 60%～72%; The specificity of its detection is also higher than other tumor markerses commonly used; Unite use with other tumor markerses, more can further improve sensitivity and the specificity of diagnosis.On detection angles, the method that is used for clinically at present mensuration CA242 mainly contains the method for exempting from of putting, ELISA method, chemoluminescence method etc.
Past is subjected to methodological restriction to put carbohydrate antigen 242 (CA242) the mensuration kit of exempting from as representative, its sensitivity and antijamming capability wretched insufficiency, there is very large drawback, basically withdraws from the market, use at present more be enzyme linked immunosorbent detection technology and chemiluminescence.It is continue Enzyme-multiplied immune technique and the emerging technology that grows up after putting immune technology the eighties that chemiluminescence rises in eighties of last century, due to its high sensitivity, high specific, while method is easy, quick, the mark bond is stable, the characteristics such as "dead" isotope damage and pollution have obtained develop rapidly in recent years.
Magnetic particle separation enzyme-linked immunoassay technology is a kind of take magnetic particle as the solid phase carrier of separating, immune magnetic particle isolation technics is combined with the enzyme linked immunosorbent detection technology and a kind of Novel immune detection method of setting up.Traditional E LISA method, the association reaction of antigen, antibody carries out on solid phase (elisa plate reacting hole) surface, and magnetic particle separation enzyme-linked immunoassay, the association reaction of antigen, antibody also carries out under the condition of approximate liquid phase, thereby reaction is fast, thoroughly.Compare with traditional E LISA and have highly sensitively, detect few advantage of used time.
The utility model content
The technical matters that the utility model solves is to provide a kind of chemiluminescence immunoassay to detect the CA242 kit, to solve the problem that proposes in the above-mentioned background technology.
The technical matters that the utility model solves realizes by the following technical solutions:
a kind of chemiluminescence immunoassay detects the CA242 kit, comprise box body and the lid that is connected with box body, be connected with the square plate that is placed in box body on described lid, be placed with the first sponge holder identical with the box body size in box body, also be placed with the second sponge holder identical with the square plate size in square plate, described the first sponge holder is provided with seven the first grooves, be placed with respectively the magnetic separation agent reagent bottle that is marked with anti-CA242 monoclonal antibody in the first groove, the CA242 monoclonal antibody reagent bottle of acridinium ester mark, increased response agent reagent bottle, the diluent reagent bottle, calibration object and quality-control product reagent bottle, the alkaline reagent solution bottle of cleaning fluid concentrate reagent bottle and above-mentioned acridinium ester effect, described the second sponge holder is provided with the second groove of at least two holding test tubes.
Further, described square plate is hinged by straight pin and lid.
Beneficial effect:
The utility model detection sensitivity and specificity are high, and have reached better performance parameter, and course of reaction is more reliable and more stable, and experimental data is effectively sensitive, when enhancing product performance, and greatly reduces cost of products.
Description of drawings
Fig. 1 is structural representation of the present utility model.
Embodiment
Of the present utility modelly to realize technological means, creation characteristic in order making, to reach purpose and effect is easy to understand, below in conjunction with concrete diagram, further set forth the utility model.
as shown in Figure 1, a kind of chemiluminescence immunoassay detects the CA242 kit, comprise box body 1 and the lid 2 that is connected with box body 1, be connected with the square plate 3 that is placed in box body 1 on described lid 2, described square plate 3 is hinged by straight pin 16 and lid 2, be placed with the first sponge holder 4 identical with box body 1 size in box body 1, also be placed with the second sponge holder 14 identical with square plate 3 sizes in square plate 3, described the first sponge holder 4 is provided with seven the first grooves 5, the interior magnetic separation agent reagent bottle 6 that is marked with anti-CA242 monoclonal antibody that is placed with respectively of the first groove 5, the CA242 monoclonal antibody reagent bottle 7 of acridinium ester mark, increased response agent reagent bottle 8, diluent reagent bottle 9, calibration object and quality-control product reagent bottle 10, the alkaline reagent solution bottle 12 of cleaning fluid concentrate reagent bottle 11 and above-mentioned acridinium ester effect, described the second sponge holder 14 is provided with the second groove 15 of five holding test tubes 13.
The utility model using method is as follows: add in sample, calibration object and quality-control product quantitative acridinium ester mark the CA242 monoclonal antibody, in conjunction with CA242 monoclonal antibody and the increased response agent of magnetic particle.37 ℃ hatch after, the CA242 monoclonal antibody of combination on magnetic particle is combined with the different epi-positions of CA242 molecule respectively from the CA242 monoclonal antibody of acridinium ester mark, forms " sandwich " structure.Direct precipitation in externally-applied magnetic field does not need centrifugal namely separable.Remove supernatant, then the compound of washing and precipitating adds the alkaline solution of stating the acridinium ester effect.Acridinium ester under alkaline solution by catalytic pyrolysis, form unsettled excited state intermediate, just send photon when the excited state intermediate is got back to ground state, form luminescence-producing reaction, the luminous intensity of light-emitting appearance detection reaction can be used, CA242 content in sample can be calculated according to typical curve.In sensing range, luminous intensity is directly proportional to CA242 concentration in sample.
Above demonstration and described ultimate principle of the present utility model and principal character and advantage of the present utility model.The technician of the industry should understand; the utility model is not restricted to the described embodiments; that describes in above-described embodiment and instructions just illustrates principle of the present utility model; under the prerequisite that does not break away from the utility model spirit and scope; the utility model also has various changes and modifications, and these changes and improvements all fall in claimed the utility model scope.Claimed scope of the present utility model is defined by appending claims and equivalent thereof.
Claims (2)
1. a chemiluminescence immunoassay detects the CA242 kit, comprise box body and the lid that is connected with box body, it is characterized in that: be connected with the square plate that is placed in box body on described lid, be placed with the first sponge holder identical with the box body size in box body, also be placed with the second sponge holder identical with the square plate size in square plate, described the first sponge holder is provided with seven the first grooves, be placed with respectively the magnetic separation agent reagent bottle that is marked with anti-CA242 monoclonal antibody in the first groove, the CA242 monoclonal antibody reagent bottle of acridinium ester mark, increased response agent reagent bottle, the diluent reagent bottle, calibration object and quality-control product reagent bottle, the alkaline reagent solution bottle of cleaning fluid concentrate reagent bottle and above-mentioned acridinium ester effect, described the second sponge holder is provided with the second groove of at least two holding test tubes.
2. a kind of chemiluminescence immunoassay according to claim 1 detects the CA242 kit, and it is characterized in that: described square plate is hinged by straight pin and lid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320316308 CN203275412U (en) | 2013-06-04 | 2013-06-04 | Kit for chemical luminescence immunoassay of CA242 (tumor marker) |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320316308 CN203275412U (en) | 2013-06-04 | 2013-06-04 | Kit for chemical luminescence immunoassay of CA242 (tumor marker) |
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| Publication Number | Publication Date |
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| CN203275412U true CN203275412U (en) | 2013-11-06 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 201320316308 Expired - Lifetime CN203275412U (en) | 2013-06-04 | 2013-06-04 | Kit for chemical luminescence immunoassay of CA242 (tumor marker) |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103789271A (en) * | 2014-01-26 | 2014-05-14 | 东北林业大学 | Hybridoma for resisting generation of CA242 monoclonal antibody and preparation of chemiluminescence immune assay kit |
| CN106771214A (en) * | 2016-11-30 | 2017-05-31 | 百奥森（江苏）食品安全科技有限公司 | Metopycide detection method and kit in a kind of poultry |
| CN108584106A (en) * | 2018-05-08 | 2018-09-28 | 佛山市尚柏科技有限公司 | A kind of microbial rapid detection reagent box |
-
2013
- 2013-06-04 CN CN 201320316308 patent/CN203275412U/en not_active Expired - Lifetime
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103789271A (en) * | 2014-01-26 | 2014-05-14 | 东北林业大学 | Hybridoma for resisting generation of CA242 monoclonal antibody and preparation of chemiluminescence immune assay kit |
| CN106771214A (en) * | 2016-11-30 | 2017-05-31 | 百奥森（江苏）食品安全科技有限公司 | Metopycide detection method and kit in a kind of poultry |
| CN108584106A (en) * | 2018-05-08 | 2018-09-28 | 佛山市尚柏科技有限公司 | A kind of microbial rapid detection reagent box |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20160406 Address after: 215163 building, No. 8, Jinfeng Road, hi tech Zone, Suzhou, Jiangsu, China Patentee after: SUZHOU HYBIOME BIOMEDICAL ENGINEERING Co.,Ltd. Address before: 215163 building, No. 8, Jinfeng Road, hi tech Zone, Suzhou, Jiangsu, China Patentee before: SUZHOU HYBIOME BIOREAGENT Co.,Ltd. |
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| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20131106 |