CN203275414U - Rapidly-detected CA19-9 kit - Google Patents
Rapidly-detected CA19-9 kit Download PDFInfo
- Publication number
- CN203275414U CN203275414U CN 201320316339 CN201320316339U CN203275414U CN 203275414 U CN203275414 U CN 203275414U CN 201320316339 CN201320316339 CN 201320316339 CN 201320316339 U CN201320316339 U CN 201320316339U CN 203275414 U CN203275414 U CN 203275414U
- Authority
- CN
- China
- Prior art keywords
- reagent bottle
- kit
- box body
- reagent
- monoclonal antibody
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Images
Landscapes
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The utility model relates to a rapidly-detected CA19-9 kit which comprises a kit body and a turnover-type kit cover, wherein the kit body and the turnover-type kit cover are connected together and are sealed through a clamping buckle; a sponge support, the size of which is the same as that of the kit body, is placed in the kit body; seven grooves are formed in the sponge support; a magnetic microsphere reagent bottle with a mark for resisting a CA19-9 monoclonal antibody, an acridinium ester-marked reagent bottle in which a CA19-9 monoclonal antibody resisting reagent is contained, a reaction enhancer reagent bottle, a diluent reagent bottle, a standard product and quality-control product reagent bottle, a cleaning fluid concentrate reagent bottle and an alkaline solution reagent bottle acted by the acridinium ester are respectively placed in the grooves. The rapidly-detected CA19-9 kit is high in detection sensitivity and specificity, so that the detection time is shortened and the performance parameter is good. Thus, the product performance is improved while the product cost is lowered greatly. The rapidly-detected CA19-9 kit is more stable and reliable in reaction process as well as sensitive and effective in experimental data, so that the service life and the detection performance of the kit are guaranteed powerfully.
Description
Technical field
The utility model relates to the immunoassay medical domain, is specially a kind of fast detecting CA19-9 kit.
Background technology
CA19-9 is the compound sugar tumor associated antigen, and molecular weight is (300～500) ten thousand.All have CA19-9 to express in the stomach of fetus, intestines, liver and pancreas, normal adult only has the expression of minute quantity at pancreas, liver, gall-bladder and lung.Clinical studies show, CA19-9 is a kind of tumor markers relevant to cancer of pancreas, carcinoma of gallbladder, colon cancer etc.CA19-9 be, clinical practice the highest to cancer of pancreas susceptibility so far at most and the tumor markers of most worthy, can reach 70%～95% to the diagnostic sensitivity of cancer of pancreas, specificity is 72%～90%.Simultaneously, the detection of CA19-9 has larger diagnostic effect to tumor in digestive tract, is used for alone or in combination diagnosis and the recurrence monitoring of malignant tumor of ovary as a kind of tumor markers.Multinomial studies show that, the level of CA19-9 and disease progression stage have correlativity, are all one of the most effective predictability indexs for the case CA19-9 that can perform the operation He can't perform the operation.In addition, CA19-9 raises and is also shown in some nonmalignant diseases, as acute and chronic pancreatitis, cholecystitis, biliary obstruction, hepatitis, cirrhosis etc., but generally can be very not high.The method that is used for clinically at present mensuration CA19-9 mainly contains the method for exempting from of putting, ELISA method, chemoluminescence method etc.
Past is subjected to methodological restriction to put CA19-9 (CA19-9) the mensuration kit of exempting from as representative, its sensitivity and antijamming capability wretched insufficiency, there is very large drawback, basically withdraws from the market, use at present more be enzyme linked immunosorbent detection technology and chemiluminescence.It is continue Enzyme-multiplied immune technique and the emerging technology that grows up after putting immune technology the eighties that chemiluminescence rises in eighties of last century, due to its high sensitivity, high specific, while method is easy, quick, the mark bond is stable, the characteristics such as "dead" isotope damage and pollution have obtained develop rapidly in recent years.
The magnetic microparticle separating chemiluminescence immunoassay technology is a kind of take magnetic particle as the solid phase carrier of separating, immune magnetic particle isolation technics is combined with the chemiluminescence immunoassay detection technique and a kind of Novel immune detection method of setting up.Traditional E LISA method, the association reaction of antigen, antibody carries out on solid phase (elisa plate reacting hole) surface, and the magnetic microparticle separating chemiluminescence immunologic detection method, the association reaction of antigen, antibody also carries out under the condition of approximate liquid phase, thereby reaction is fast, thoroughly.Compare with traditional E LISA and have highly sensitively, detect few advantage of used time.
The utility model content
The technical matters that the utility model solves is to provide a kind of fast detecting CA19-9 kit, to solve the problem that proposes in the above-mentioned background technology.
The technical matters that the utility model solves realizes by the following technical solutions:
fast detecting CA19-9 kit, the flip-shell lid that comprises box body and be connected with box body, seal by buckle between lid and box body, be placed with the sponge holder identical with the box body size in box body, described sponge holder is provided with seven grooves, place respectively the magnetic microsphere reagent bottle that is marked with anti-CA19-9 monoclonal antibody in groove, the anti-CA19-9 monoclonal antibody reagent of acridinium ester mark reagent bottle, increased response agent reagent bottle, the diluent reagent bottle, standard items and quality-control product reagent bottle, the alkaline reagent solution bottle of cleaning fluid concentrate reagent bottle and above-mentioned acridinium ester effect.
Further, the mentioned reagent bottle surface is equipped with volume markings, and the mentioned reagent bottle surface also is provided with decorative pattern.
Compare with conventional art, the beneficial effects of the utility model are as follows:
1, detection sensitivity and specificity are high, have shortened detection time, have better performance parameter; When enhancing product performance, and greatly reduce cost of products.
2, course of reaction is more reliable and more stable, and experimental data is effectively sensitive, and giving the use effect phase of this kit and detecting performance provides powerful guarantee.
Description of drawings
Fig. 1 is structural representation of the present utility model;
Fig. 2 is the structural representation of the utility model reagent bottle.
Embodiment
Of the present utility modelly to realize technological means, creation characteristic in order making, to reach purpose and effect is easy to understand, below in conjunction with concrete diagram, further set forth the utility model.
as shown in Figure 1, fast detecting CA19-9 kit, comprise box body 1 and the flip-shell lid 2 that is connected with box body 1, seal by buckle 3 between lid 2 and box body 1, be placed with the sponge holder 4 identical with the box body size in box body 1, described sponge holder 4 is provided with seven grooves 5, the interior magnetic microsphere reagent bottle 6 that is marked with anti-CA19-9 monoclonal antibody of placing respectively of groove 5, the anti-CA19-9 monoclonal antibody reagent of acridinium ester mark reagent bottle 7, increased response agent reagent bottle 8, diluent reagent bottle 9, standard items and quality-control product reagent bottle 10, the alkaline reagent solution bottle 12 of cleaning fluid concentrate reagent bottle 11 and above-mentioned acridinium ester effect.The mentioned reagent bottle surface is equipped with volume markings 13, and the mentioned reagent bottle surface has the depression 14 of being convenient to hold.
The utility model using method is as follows: add in sample, calibration object and quality-control product the anti-CA19-9 monoclonal antibody of quantitative acridinium ester mark, in conjunction with anti-CA19-9 monoclonal antibody and the increased response agent of magnetic particle.37 ℃ hatch after, on magnetic particle, the anti-CA19-9 monoclonal antibody of combination is combined with the different epi-positions of CA19-9 molecule respectively from the anti-CA19-9 monoclonal antibody of acridinium ester mark, forms " sandwich " structure.Direct precipitation in externally-applied magnetic field does not need centrifugal namely separable.Remove supernatant, then the compound of washing and precipitating adds the alkaline solution of acridinium ester effect.Acridinium ester under the alkaline solution effect by catalytic pyrolysis, form unsettled excited state intermediate, just send photon when the excited state intermediate is got back to ground state, form luminescence-producing reaction, the luminous intensity of light-emitting appearance detection reaction can be used, CA19-9 content in sample can be calculated according to typical curve.In sensing range, luminous intensity is directly proportional to CA19-9 concentration in sample.
Above demonstration and described ultimate principle of the present utility model and principal character and advantage of the present utility model.The technician of the industry should understand; the utility model is not restricted to the described embodiments; that describes in above-described embodiment and instructions just illustrates principle of the present utility model; under the prerequisite that does not break away from the utility model spirit and scope; the utility model also has various changes and modifications, and these changes and improvements all fall in claimed the utility model scope.Claimed scope of the present utility model is defined by appending claims and equivalent thereof.
Claims (2)
1. fast detecting CA19-9 kit, the flip-shell lid that comprises box body and be connected with box body, it is characterized in that: seal by buckle between lid and box body, be placed with the sponge holder identical with the box body size in box body, described sponge holder is provided with seven grooves, place respectively the magnetic microsphere reagent bottle that is marked with anti-CA19-9 monoclonal antibody in groove, the anti-CA19-9 monoclonal antibody reagent of acridinium ester mark reagent bottle, increased response agent reagent bottle, the diluent reagent bottle, standard items and quality-control product reagent bottle, the alkaline reagent solution bottle of cleaning fluid concentrate reagent bottle and above-mentioned acridinium ester effect.
2. fast detecting CA19-9 kit according to claim 1, it is characterized in that: the mentioned reagent bottle surface is equipped with volume markings, and the mentioned reagent bottle surface has the depression of being convenient to hold.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320316339 CN203275414U (en) | 2013-06-04 | 2013-06-04 | Rapidly-detected CA19-9 kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320316339 CN203275414U (en) | 2013-06-04 | 2013-06-04 | Rapidly-detected CA19-9 kit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203275414U true CN203275414U (en) | 2013-11-06 |
Family
ID=49505793
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201320316339 Expired - Lifetime CN203275414U (en) | 2013-06-04 | 2013-06-04 | Rapidly-detected CA19-9 kit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203275414U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107389946A (en) * | 2017-08-25 | 2017-11-24 | 北京健安生物科技有限公司 | Sugar antigen CA19 9 determines kit and its detection method |
| CN114605550A (en) * | 2020-12-08 | 2022-06-10 | 东莞市朋志生物科技有限公司 | anti-CA 19-9 antibody, application thereof and kit for detecting CA19-9 |
-
2013
- 2013-06-04 CN CN 201320316339 patent/CN203275414U/en not_active Expired - Lifetime
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107389946A (en) * | 2017-08-25 | 2017-11-24 | 北京健安生物科技有限公司 | Sugar antigen CA19 9 determines kit and its detection method |
| CN114605550A (en) * | 2020-12-08 | 2022-06-10 | 东莞市朋志生物科技有限公司 | anti-CA 19-9 antibody, application thereof and kit for detecting CA19-9 |
| CN114605550B (en) * | 2020-12-08 | 2023-09-22 | 东莞市朋志生物科技有限公司 | Antibodies against CA19-9, uses thereof and kit for detecting CA19-9 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Yuan et al. | Synthesis of a terbium fluorescent chelate and its application to time-resolved fluoroimmunoassay | |
| EP3242133B1 (en) | Composition and system for separating and detecting alpha-fetoprotein variant and use thereof | |
| CN105738457A (en) | Preparation method and application of magnetic electrochemical immunosensor for simultaneously detecting two tumor markers based on metal substrate sign | |
| CN104597019A (en) | In-situ composite system based on carbon quantum dot/manganese dioxide nanometer sheet layer and using method for detecting content of glutathione | |
| CN105301241B (en) | Preparing method and application of sandwich type electrochemical luminescence immunosensor for detecting tumor marker | |
| CN104568923A (en) | Method and kit for detecting circulating tumor cell antigens in peripheral blood through electrochemical luminescence detection | |
| CN203275414U (en) | Rapidly-detected CA19-9 kit | |
| Chan et al. | The use of immunohistochemistry in liver tumors | |
| CN204374216U (en) | A kind of kit of quick detection hepatitis b virus s antigen | |
| CN103499692B (en) | Golgiosome protein GP 73 quantitative determination reagent kit and detection method thereof | |
| Chen et al. | Evaluation of Serum Des‐Gamma‐Carboxy Prothrombin for the Diagnosis of Hepatitis B Virus‐Related Hepatocellular Carcinoma: A Meta‐Analysis | |
| CN104459132A (en) | Preparation method and application of pancreatic cancer immunosensor based on gold electrodeposition and Au@Ag/CuO-GS as markers | |
| CN104089950A (en) | Method for visually detecting antigen-antibody reaction and application of method | |
| CN102636649A (en) | Kit for detecting carcinoembryonic antigen based on antibody functionalized magnetic nanometer material and up-conversion fluorescence nanometer material | |
| CN106366197A (en) | HER2, EGFR, EpCAM and MUC1 multiple antibody immunomagnetic bead and preparation method thereof | |
| US20150233932A1 (en) | Methods, Systems, and Compositions for Enrichment of Rare Cells | |
| CN203275412U (en) | Kit for chemical luminescence immunoassay of CA242 (tumor marker) | |
| CN101865849A (en) | Weak light detection device | |
| CN102279264A (en) | Preparation process for chip with 48 malignant tumor marker antibodies | |
| CN106596523A (en) | Discovery and applications of sCD40L liver cancer early screening and diagnosis marker | |
| CN203164191U (en) | Rapid carcino embryonie antigen (CEA) detection kit | |
| CN106119340B (en) | A kind of detection method, detection reagent and the detection kit of de- γ carboxyls factor | |
| CN109580951A (en) | The kit and its application method of multispecific antibody joint-detection early liver cancer marker | |
| CN103033623A (en) | Human M2 type pyruvate kinase chemiluminescence immune assay kit and preparation method | |
| CN203275413U (en) | Rapidly-detected CA50 kit |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20160108 Address after: 215163 building, No. 8, Jinfeng Road, hi tech Zone, Suzhou, Jiangsu, China Patentee after: SUZHOU HYBIOME BIOMEDICAL ENGINEERING Co.,Ltd. Address before: 215163 building, No. 8, Jinfeng Road, hi tech Zone, Suzhou, Jiangsu, China Patentee before: SUZHOU HYBIOME BIOREAGENT Co.,Ltd. |
|
| CX01 | Expiry of patent term |
Granted publication date: 20131106 |
|
| CX01 | Expiry of patent term |