CN203290151U - Novel closed-type refrigerating superfine tube carrier for rare sperms - Google Patents
Novel closed-type refrigerating superfine tube carrier for rare sperms Download PDFInfo
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- CN203290151U CN203290151U CN2013200298007U CN201320029800U CN203290151U CN 203290151 U CN203290151 U CN 203290151U CN 2013200298007 U CN2013200298007 U CN 2013200298007U CN 201320029800 U CN201320029800 U CN 201320029800U CN 203290151 U CN203290151 U CN 203290151U
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- inner tube
- tube
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- freezing
- sperm
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Abstract
The utility model relates to a novel closed-type refrigerating superfine tube carrier for rare sperms. The novel closed-type refrigerating superfine tube carrier is provided with an inner tube; the inner tube is provided with a thick end and a thin end; the inner tube is externally provided with an outer casing tube; the inner tube and the outer casing tube can slide relatively; and the length of the inner tube is greater than the length of the outer casing tube. A trace inner tube is adopted as a seminal fluid refrigerating and storing container for the novel closed-type refrigerating superfine tube carrier provided by the utility model, and the seminal fluid refrigerating and storing container is small in volume, and sperms are easy to find after recovery; because the inner tube is thin in wall, and is small in volume, and the outer casing tube is made of stainless steel materials, the superfine tube carrier is rapid in temperature reduction speed rate, and can pass through the freezing point rapidly in refrigeration; in recovery, due to the heat conduction of the inner tube, the best temperature for recovery is rapidly reached, and after recovery, limited sperms can be selected, and can be directly used for micro fertilization bank ART (assisted reproductive technology) treatment; and the outer casing tube of the superfine tube carrier can effectively protect the inner tube, and can prevent the inner tube from being fractured and cracked. The novel closed-type refrigerating superfine tube carrier has a good refrigerating effect, is simple in structure, is convenient to use, and is suitable for clinical popularization and use.
Description
Technical field
The utility model relates to spermatozoa cryopreservation device technique field, specifically, is the freezing ultra-fine pipe carrier of a kind of rare sperm novel closed-type.
Background technology
Freezing (2-3 bar/LPF) to rare sperm, the report of the various freezing carriers of present common use, Cohen and Desai adopt respectively oolemma as the frozen rare sperm of freezing carrier, and it is the sperm that the freezing testis of carrier, epididymis obtain that Nawroth adopts freezing ring; The method report that Lu Guang Xiu etc. " not adding the freezing rare sperm of method for vitrification of cryoprotector " is arranged again subsequently.Although but above-mentioned research succeeds and cuts both ways.There is foreign protei in oolemma, may have the risk of biological pollution; Freezing ring price is more expensive, and it is freezing that the sperm that testis, epididymis are obtained may need several freezing rings to carry out, and cost increases.Do not add the freezing straw of the opening of cryoprotector, exist the problem that liquid nitrogen pollutes.The storage tube of cryopreservation sperm, freezing straw etc. are not all so satisfactory to functional parameters such as the sperm motility after the Cryopreservation of rare sperm, sperm morphology, Sperm acrasomal integvity and DNA break indexes at present.Therefore, need badly and want a kind of frozen effective, easy to operate, simple in structure, can solve the ultramicron spermatozoa cryopreservation device of above-mentioned safety and quantity problem.
The utility model content
The purpose of this utility model is for deficiency of the prior art, provides a kind of rare sperm novel closed-type freezing ultra-fine pipe carrier.
For achieving the above object, the technical scheme taked of the utility model is:
The freezing ultra-fine pipe carrier of a kind of rare sperm novel closed-type, be provided with inner tube, and described inner tube is provided with butt end and taper end, and described interior tube outside is provided with outer tube, and inner tube and outer tube can relative slidings.
The length of described inner tube is greater than the length of outer tube.
The body of described outer tube is provided with breach, and an end of outer tube is necking end, and described breach is near described necking end.
Described outer tube is the outer tube of stainless steel material preparation.
Described inner tube is the inner tube of ionic polymerization resin material preparation.
The internal diameter of the butt end of described inner tube is 0.6～0.8 millimeter, and the internal diameter of taper end is 0.2～0.28 millimeter.
The wall thickness of described inner tube is 0.07 millimeter.
The volume of described inner tube is 50 microlitres.
The utility model advantage is:
1, the freezing ultra-fine pipe carrier of the rare sperm novel closed-type of the utility model, adopt micro-inner tube to freeze storage container as seminal fluid, totally-enclosed preservation, and its volume is little, and after recovery, sperm is easily looked for;
2, this ultra-fine pipe carrier is because inner tubal wall is thin, volume is little, outer tube is that stainless steel material is made, when freezing, rate of temperature fall is fast, can pass through rapidly freezing point, during recovery, the hot conducting energy of inner tube reaches rapidly the recovery optimum temperature, therefore limited sperm can be picked out after recovery and directly carry out the capable ART treatment of Microfertilization;
3, the outer tube of this ultra-fine pipe carrier can effectively be protected inner tube, prevents that it from fractureing, collision cleft;
4, the freezing ultra-fine pipe carrier of this rareness sperm novel closed-type is frozen effective, simple in structure, easy to use, is fit to clinical expansion and uses.
Description of drawings
Accompanying drawing 1 is the structural representation of the inner tube of the freezing ultra-fine pipe carrier of the rare sperm novel closed-type of the utility model.
Accompanying drawing 2 is structural representations of the outer tube of the freezing ultra-fine pipe carrier of the rare sperm novel closed-type of the utility model, and A figure is front view, and B figure is vertical view.
Accompanying drawing 3 is structural representations of the freezing ultra-fine pipe carrier of the rare sperm novel closed-type of the utility model, and (a) figure is that outer tube is positioned at the inner tube butt end, and (b) figure is that outer tube is positioned at the inner tube taper end.
The Reference numeral and the part that relate in accompanying drawing are as follows:
1. inner tube 11. inner tube butt ends
12. inner tube taper end 2. outer tubes
21. breach 22. outer tube necking ends
Embodiment
Below in conjunction with case study on implementation and with reference to accompanying drawing, the utility model is further described.
The freezing ultra-fine pipe carrier of the rare sperm novel closed-type of the utility model comprises inner tube 1 and outer tube 2.Please refer to Fig. 1, Fig. 1 is the structural representation of the inner tube of the freezing ultra-fine pipe carrier of rare sperm novel closed-type, and described inner tube 1 is provided with inner tube butt end 11 and inner tube taper end 12, and inner tube butt end 11 and inner tube taper end 12 are one-body molded, junction is smooth, the both ends open of inner tube 1.Please refer to Fig. 2, Fig. 2 is the structural representation of the outer tube of the freezing ultra-fine pipe carrier of rare sperm novel closed-type, A figure is front view, B figure is vertical view, the body of described outer tube 2 is provided with breach 21, one end of outer tube 2 is necking end 22, and described breach 21 is near described necking end 22, and the two ends of outer tube 2 are opening also.
Please refer to Fig. 3, Fig. 3 is the structural representation of the freezing ultra-fine pipe carrier of rare sperm novel closed-type, and (a) figure is that outer tube 2 is positioned at inner tube butt end 11, and (b) figure is that outer tube 2 is positioned at inner tube taper end 12.Described outer tube 2 can be enclosed within the outside of inner tube 1, and inner tube 1 and outer tube 2 can relative slidings, and outer tube 2 also can unload from inner tube 1, and the length of inner tube 1 is greater than the length of outer tube 2.When drawing seminal fluid and blowing out seminal fluid, outer tube 2 is positioned at inner tube butt end 11, after the seminal fluid absorption is complete, outer tube 2 is slided to inner tube taper end 12, can protect inner tube taper end 12, avoids it to be damaged.See through described breach 21 and can see the situation of inner tube 1.
Need to prove, described outer tube 2 is the stainless steel metal outer tube, and intensity is high, and is not yielding, and can reuse; Described inner tube 1 is the inner tube of ionic polymerization resin material preparation, easy moulding during preparation, and can see the situation that pipe is interior from outside; The internal diameter of described inner tube butt end 11 is 0.6～0.8 millimeter, and the internal diameter of inner tube taper end 12 is 0.2～0.28 millimeter; The wall thickness of inner tube 1 is 0.07 millimeter, and the volume of inner tube 1 is 50 microlitres.
The using method of the freezing ultra-fine pipe carrier of the rare sperm novel closed-type of the utility model comprises the following steps:
(1) sperm freezing
With periphery obtain less, weak semen sample or operation testis sample or the epididymis puncture fluid obtained be placed in the culture dish that contains (Hepes-) m-HTF; Few, the weak semen sample of periphery carries out gradient centrifugation after semen liquefaction processes by routine clinical treating method, if testis tissue or epididymis puncture fluid, the syringe needle of 1ml syringe is curved after the calcination of alcolhol burner flame envelope " 7 " font, peel off seminiferous tubule under stereoscope, cell in seminiferous tubule is flowed out, blow and beat gently with pasteur pipet, the androgone tissue is dispelled, collect the testicular cell suspension.
1ml 90% density gradient liquid is added 15ml centrifuge tube bottom, then along tube wall, slowly add successively 1ml 45% density gradient liquid, note not mixing; The testicular cell suspension of the semen sample of liquefaction or collection and treatment is slowly added density gradient liquid top.After the centrifugal 25min of 300～500g, draw respectively the lower sediment cell with pasteur pipet, be placed in small test tube, add respectively 2～3ml medium, mix the centrifugal 5min of rear 400g, suck the supernatant liquid in test tube, add respectively approximately 0.5ml medium in precipitation, mix and be placed in Tissue Culture Dish, 37 ℃, 5%CO
2Cultivated in incubator 24～48 hours, to be seen freezing to carrying out after sperm has activity.
Less, weak seminal fluid will need freezing sperm to add 2～3ml medium to mix gently after processing, low-speed centrifugal 10 minutes, suck supernatant.The sperm of precipitation is mixed gently, in ratio and the freezing liquid of 1:1, fully mix, it is divided into droplet of 50ul.The 1ml syringe is connected on the opening of inner tube butt end 11 of the ultra-fine pipe carrier of the utility model, sperm and freezing liquid are sucked in inner tube 1 in the lump; The testicular sperm freezing method is identical with periphery sperm freezing step.Draw complete after, pull up syringe, the two-port of inner tube 1 is sealed, then the outer tube of activity 2 is slided on inner tube taper end 12, to protect the narrow of ultra-fine pipe carrier.The ultra-fine pipe carrier that seminal fluid is housed is placed in two polyethylene cannulas and tips upside down on freezing support, carry out patient and data mark.Directly put stifling balance in-140 ℃ of liquid nitrogen and after 3 hours, freezing support is shifted out from the liquid nitrogen of-140 ℃, be placed in-196 ℃ of liquid nitrogen containers and preserve.
(2) sperm thaws
While thawing, the freezing support that is placed with ultra-fine pipe carrier, by taking out in-196 ℃ of liquid nitrogen containers, was put in 37 ℃ of water baths rewarming 5 minutes.Then take out ultra-fine pipe carrier, the globule on the ultra-fine pipe carrier of wiping.In the laminar flow super-clean bench, outer tube 2 is moved on to inner tube butt end 11, expose inner tube taper end 12, cut off the sealing at inner tube 1 two ends, be connected to the 1ml syringe on the opening of inner tube butt end 11, then the preservation liquid that is mixed with sperm in the inner tube of ultra-fine pipe carrier 1 blown out gently, splash in the recovery drop that pre-temperature is good in advance, the recovery ware is moved into 37 ℃, 5%CO
2Cultivated in incubator 10～30 minutes.Take out finally culture fluid and be inverted Microscopic observation, counting sperm survival rate, the rate of recovery etc.
To with the ultra-fine pipe carrier of the utility model and 0.25 milliliter of straw, 0.5 milliliter of straw, having carried out comparing before and after the sperm freezing recovery, every kind of frozen carrier arranges 30 routine samples, compare its sperm motility, sperm morphology natural rate of interest, Sperm acrasomal integvity, DNA fragment with spermatozoon rate after Cryopreservation, result is listed in table 1 and table 2 in detail.
The refrigerating effect of 0.5 milliliter of straw of table 1 and the ultra-fine pipe carrier of the utility model relatively
The refrigerating effect of 0.25 milliliter of straw of table 2 and the ultra-fine pipe carrier of the utility model relatively
Can find out from table 1, table 2, use the ultra-fine pipe carrier of the utility model better than the frozen effect of 0.5 milliliter of straw and 0.25 milliliter of straw.
The freezing ultra-fine pipe carrier of the rare sperm novel closed-type of the utility model, adopt micro-inner tube to freeze storage container as seminal fluid, totally-enclosed preservation, and its volume is little, and after recovery, sperm is easily looked for; This ultra-fine pipe carrier is because inner tubal wall is thin, volume is little, outer tube is that stainless steel material is made, when freezing, rate of temperature fall is fast, can pass through rapidly freezing point, therefore during recovery, the hot conducting energy of inner tube reaches rapidly the recovery optimum temperature, limited, the great-hearted sperm of tool can be picked out and is directly used in the capable ART treatment of Microfertilization after the recovery; The outer tube of this ultra-fine pipe carrier can effectively be protected inner tube, prevents that it from fractureing, collision cleft; The freezing ultra-fine pipe carrier of this rareness sperm novel closed-type frozen effective, simple in structure, easy to use, be fit to clinical expansion and use.
The above is only preferred embodiment of the present utility model; should be pointed out that for those skilled in the art, under the prerequisite that does not break away from the utility model principle; can also make some improvement and supplement, these improvement and supplement and also should be considered as protection domain of the present utility model.
Claims (8)
1. the freezing ultra-fine pipe carrier of rare sperm novel closed-type, be provided with inner tube, it is characterized in that, described inner tube is provided with butt end and taper end, and described interior tube outside is provided with outer tube, and inner tube and outer tube can relative slidings.
2. the freezing ultra-fine pipe carrier of rare sperm novel closed-type according to claim 1, is characterized in that, the length of described inner tube is greater than the length of outer tube.
3. the freezing ultra-fine pipe carrier of rare sperm novel closed-type according to claim 1, is characterized in that, the body of described outer tube is provided with breach, and an end of outer tube is necking end, and described breach is near described necking end.
4. the freezing ultra-fine pipe carrier of rare sperm novel closed-type according to claim 1, is characterized in that, described outer tube is the outer tube of stainless steel material preparation.
5. the freezing ultra-fine pipe carrier of rare sperm novel closed-type according to claim 1, is characterized in that, described inner tube is the inner tube of ionic polymerization resin material preparation.
6. the freezing ultra-fine pipe carrier of rare sperm novel closed-type according to claim 1, is characterized in that, the internal diameter of the butt end of described inner tube is 0.6～0.8 millimeter, and the internal diameter of taper end is 0.2～0.28 millimeter.
7. the freezing ultra-fine pipe carrier of rare sperm novel closed-type according to claim 1, is characterized in that, the wall thickness of described inner tube is 0.07 millimeter.
8. the freezing ultra-fine pipe carrier of rare sperm novel closed-type according to claim 1, is characterized in that, the volume of described inner tube is 50 microlitres.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013200298007U CN203290151U (en) | 2013-01-21 | 2013-01-21 | Novel closed-type refrigerating superfine tube carrier for rare sperms |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013200298007U CN203290151U (en) | 2013-01-21 | 2013-01-21 | Novel closed-type refrigerating superfine tube carrier for rare sperms |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203290151U true CN203290151U (en) | 2013-11-20 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2013200298007U Expired - Lifetime CN203290151U (en) | 2013-01-21 | 2013-01-21 | Novel closed-type refrigerating superfine tube carrier for rare sperms |
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| Country | Link |
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| CN (1) | CN203290151U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105660607A (en) * | 2016-03-18 | 2016-06-15 | 冯贵雪 | Fertility preserving method of completely immovable sperms |
-
2013
- 2013-01-21 CN CN2013200298007U patent/CN203290151U/en not_active Expired - Lifetime
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105660607A (en) * | 2016-03-18 | 2016-06-15 | 冯贵雪 | Fertility preserving method of completely immovable sperms |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term |
Granted publication date: 20131120 |
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| CX01 | Expiry of patent term |