CN203195700U - Fast blood uric acid measuring instrument - Google Patents
Fast blood uric acid measuring instrument Download PDFInfo
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- CN203195700U CN203195700U CN 201320081103 CN201320081103U CN203195700U CN 203195700 U CN203195700 U CN 203195700U CN 201320081103 CN201320081103 CN 201320081103 CN 201320081103 U CN201320081103 U CN 201320081103U CN 203195700 U CN203195700 U CN 203195700U
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Abstract
The utility model provides a fast blood uric acid measuring instrument which comprises a blood sampling device, a display device and a control device, wherein the control device is connected with the blood sampling device and the display device, the blood sampling device comprises a blood sampling test strip and a test strip connector, the control device comprises a circuit amplifying module, an operating button, a power module, a current collecting module, a processor module and an analog-digital conversion module, the display device is a display screen, the blood sampling test strip is connected with the circuit amplifying module through the test strip connector, the output end of the circuit amplifying module is connected with the input end of the current collecting module, the output end of the current collecting module is connected with the input end of the analog-digital conversion module, the output end of the analog-digital conversion module is connected with the input end of the processor module, the output end of the processor module is connected with the display screen, and the operating button and the power module are connected with the processor module. The fast blood uric acid measuring instrument is convenient to operate, high in accuracy, suitable for accurate measurement of uric acid, capable of carrying out the measurement without the need of processing a sample in advance, low in price, independent of professional analysts, simple in operation, suitable for analysis work of a single batch sample and being popularized for household use, beneficial to the miniaturization of the instrument, and suitable for being operated by non-professional people.
Description
Technical field
This utility model relates to field of medical devices, relates in particular to a kind of quick survey blood uric acid instrument.
Background technology
Gout is owing to the purine substance metabolism disorder, and uric acid produces too much and drains minimizing, makes serum uric acid level continue to increase, and urate crystal is deposited on one group of metabolic disease due to the soft tissue.It is the biochemical basis of gout that uric acid level raises, and hyperuricemia is the state in early stage that gout takes place, and gout must be accompanied hyperuricemia.In recent years, the sickness rate of gout and hyperuricemia is in rising trend, and age of onset is and becomes younger, and has brought huge burden to society, and closely related with the generation of hypertension, hyperuricemia, atherosclerosis, obesity, insulin resistant, become the commonly encountered diseases of middle-aging male.But existing detection uric acid method complexity is loaded down with trivial details, though method is had nothing in common with each other, all consuming time longer, required related reagent is various, has also increased the cost of detection by quantitative blood uric acid virtually simultaneously.
The method of the mensuration uric acid of having reported at present has gravimetric method, titrimetry, reducing process and enzyme process etc.Gravimetric method and titrimetry complex operation, and accuracy is low, is unsuitable for the accurate mensuration of uric acid; Usually adopt reducing process and enzymic colorimetric clinically.Detect with reducing process, uric acid is oxidized to carbamide by phosphotungstic acid under alkali condition, and phosphotungstic acid is reduced into tungsten blue, and latter's growing amount is directly proportional with uric acid content.Its shortcoming is: the ascorbic acid in the blood sample easily disturbs.Sample must carry out deproteinization in advance to be handled, and needs expensive device and specialty analysis personnel.Enzyme process is divided into enzyme connection photometry and enzyme connection electrochemical process, the former is usually used in the automatic biochemistry analyzer of hospital, is suitable for the analysis operation of sample in enormous quantities, but is serum or blood plasma through centrifugalize before the hemanalysis, require professional and special reagent, be unsuitable for using in the family.
The most frequently used in the prior art is that high performance liquid chromatography (High Performance Liquid Chromatography HPLC) claims " high pressure liquid chromatography ", " high-speed liquid chromatography ", " high separation liquid chromatograph ", " column chromatography in modern age " etc. again.High performance liquid chromatography is a chromatographic important branch, be mobile phase with liquid, adopt the high pressure transfusion system, the mobile phase such as mixed solvent, buffer that will have the single solvent of opposed polarity or different proportion pumps into the chromatographic column that immobile phase is housed, in post each composition separated after, enter detector and detect, thereby realization is to the analysis of sample.
High performance liquid chromatography is to use always, more basic uric acid detection method, its operation mainly to note following some: 1. the preparation of standard reference material and sample is handled with complete: 1) standard reference material preparation: with the mixed standard solution S1 of uric acid with 11. 0mmol/ L, with S1 doubling dilution 5 times to S6.2) sample collection: collect experimenter 24h urine, anticorrosion with toluene, accurate recording urine amount is got about 5ml behind the mixing, while extracting vein blood 3ml, and in time separation of serum places under-20 e with urine, in all inner analysis.3) accurately draw serial hybrid standard liquid, urine 0. 1ml of serum and 40 times of dilutions, drip 1. 9ml methanol mixing on the vortex oscillation device, in the centrifugal 10min of 6000r/ min, get supernatant 1. 2ml and place clean tube, under nitrogen current, dry up, redissolve with mobile phase 0. 3ml before analyzing, whirlpool mixes 1min, and auto injection 40ul carries out chromatography.2. chromatographic condition: analytical column: 0. 6cm pre-column Shim-packCLC G-ODS (4) Shim-pack CLC-ODS 15cm); Mobile phase 0. 02mol/ L KH2 PO4.3. transfer PH 3. 2 with phosphoric acid; Flow velocity 1ml/ min, column temperature 35e AUFS 0. 001; Wavelength 233nm.
Above-mentioned the deficiencies in the prior art part is:
1 needs complicated pretreatment, comprises preparation and the processing of standard reference material and sample
HPLC is widely used, develops a kind of analytical method rapidly in the present biological sample pharmaceutical analysis, though its detection method is simple, because biological sample component complexity particularly contains macromole impurity such as a large amount of protein, cause that easily stigma stops up and rising post pressure; Sample Chinese medicine concentration is lower in addition, needs further enrichment just can detect, and all needs sample is carried out suitable pre-treatment, so that drug purification, enrichment.Usual way is to carry out liquid-liquid extraction, but exist such as easy generation emulsifying, shortcoming such as impurity is more, the response rate is low, the sample size that needs is big.Under the situation about having, shorten experimental period, can cause some difficult separate substances to separate.And it also has its shortcoming in the in-house facility design.
2 need special detecting instrument (as UV-detector) and complicated post switching device
3 used reagent are generally all poisonous
4 anti-interferences are relatively poor, the influence of sample degraded or reason such as sample is impure, sample evaporation, sample pre-treatments are improper, internal standard substance mis-arrange, sample introduction problem, peak height peak area integrated value are inaccurate when accuracy is subject to sample pretreatment.The purity of mobile phase solvent and quality have material impact to analysis result and instrument itself.Various trace impurities in the solvent not only can cause higher baseline and ghost peak, and then influence the qualitative and quantitative analysis result, and may pollute detached dowel and block system, cause instrument to break down.
5 complicated operations need special testing staff
6 prices are higher
Be diverse technical scheme and this utility model adopts with it.The complex sample preparation that does not need early stage, highly sensitive, directly get several milliliters of finger blood, electrode preparation is simple, and response is fast, and is easy to use, and understandable being convenient to easy and simple to handle promoted.And price of the present invention is lower, and is easy to carry, is convenient to extend in the family and uses, and investigates the electrochemical behavior of uric acid on modified electrode and shows that this modified electrode has improved the sensitivity of current-responsive.Have choose, sensitivity and stable advantages of higher.
Under optimized conditions, the oxidation peak current of uric acid on modified electrode significantly strengthens, and has characteristics such as stability and favorable reproducibility.
This utility model provides a kind of a kind of equipment of measuring the blood uric acid content easily and fast based on family health care and hospital application at different levels, reduces patient and goes the inconvenience of hospital and reduce the patient medical spending.Enzyme connection electrochemical process is particularly conducive to the miniaturization of instrument and is suitable for layman's operation.The Applied Electrochemistry method is measured the concentration of human body blood and urine uric acid, is a kind of accurate, quick, easy method, no matter is aspect the medicine control to the quantitative analysis of uric acid in the blood of human body, and is still aspect clinical diagnosis, all significant.
The utility model content
The technical problems to be solved in the utility model is to overcome above-mentioned deficiency, a kind of quick survey blood uric acid instrument is provided, by adopting the potassium ferricyanide as mediator, urate oxidase is fixed on the carbon electrodes that sodium carboxymethyl cellulose was handled, be developed into a kind of uric acid biosensor.Record the electric current that chemical reaction discharges by three-electrode system, measure the content of uric acid in the detected solution indirectly.The technical solution of the utility model comprises blood-taking device, display device, and control device, control device links to each other with blood-taking device, display device.Blood-taking device comprises blood sampling strip and strip interface; Control device comprises the amplifying circuit module, action button, power module, current acquisition module, processor module and analog-to-digital conversion module; Display device is display screen.The blood sampling strip is connected with the amplifying circuit module by the strip interface, the output of amplifying circuit module is connected with the input of current acquisition module, the output of current acquisition module is connected with the input of analog-to-digital conversion module, the output of analog-to-digital conversion module is connected with the input of processor module, and the output of processor module is connected with display screen; Action button and power module are connected with processor module.
The blood sampling strip comprises dielectric base, be provided with working electrode, reference electrode and counter electrode at insulation base, working electrode, reference electrode and counter electrode are drawn lead and contact, cover the insulating barrier on partial electrode, lead and the part contact, be arranged on the reagent layer on the working electrode that do not cover insulating barrier, reference electrode, the counter electrode, paste pad at the edge of reagent layer and add a cover hydrophilic film, by reagent layer, pad and hydrophilic film surround the blood sample reaction chamber.Lead and contact are conductive material, and working electrode, reference electrode, counter electrode are the carbon electrodes that is printed on dielectric base with the silk screen technology.Lead is the silver-colored rail that is printed on the dielectric base, and contact is the carbon-coating that is printed on the dielectric base.Reagent layer comprises bovine serum albumin, electron mediator, binding agent and PH buffer agent.Spacer thickness is the 70-120 micron, and the spacing of two pads is 2 millimeters.The size of blood sample reaction chamber has limited the sample size of blood, makes mensuration more accurate, and hydrophilic film can be accelerated the suction of blood, shortens minute.Electron mediator is the potassium ferricyanide.This blood sampling strip can effectively be removed the interference of other compositions in the blood, can measure uric acid concentration in the whole blood rapidly and accurately, has self-priming sample introduction function, and is easy to use; And manufacture method is simple, is particularly suitable for producing in enormous quantities.Blood sampling strip of the present utility model adopts three utmost point electrical measurement systems, the electric current that forms is through the strip interface, flow to the amplifying circuit module, after the current acquisition module is gathered current signal, flow to processor module by the analog-to-digital conversion module processing, processor module carries out being presented on the display screen after set converse routine calculates concentration.Analog-to-digital conversion module can be analog-digital converter.
Preferred control device can include real-time clock module, and the output of described processor module is connected with the input of real-time clock, and the output of real-time clock is connected with display screen.Real-time clock module can adopt the s-3530A real-time timepiece chip.This real-time clock module has the characteristics of high-precision low-power consumption, work crystal oscillator frequency 32K, and be provided with energy-saving mode, and can when the blood uric acid instrument is idle, make it enter energy-saving mode, save battery electric quantity.And calculate the leap year automatically, and represent the date time data with the binary-coded decimal form, for the read-write of processor module provides great convenience.
Preferred control device comprises power switching module, is connected with power module and processor module.The designing power supply on-off circuit, when when shutdown can be directly by the power module power supply except processor module and real-time clock, the power supply of other modules is all cut off, make processor module and real-time clock module enter dormancy or electricity-saving state then, can save the power consumption of standby greatly, extend working time.Waking up by interrupting realizing that produce a button and interrupt when pressing the specific operation button, wake up process device module is other module energized also thus, the rearming of blood uric acid instrument of processor module.
Preferred processor module is provided with memorizer, is used for storage of measurement data.
The beneficial effect that this utility model compared with prior art has is:
1. easy and simple to handle, accuracy is higher relatively, is suitable for the accurate mensuration of uric acid
2. sample need not to handle in advance, and equipment price is lower
3. need not the specialty analysis personnel, easy grasp simple to operate
4. be suitable for the analysis operation of single sample batches, be beneficial to be generalized in the family and use
5. the miniaturization that is conducive to instrument is suitable for layman's operation.
Description of drawings
Fig. 1 is this utility model structure drawing of device;
Fig. 2 is this utility model modular structure figure;
Fig. 3 is this utility model blood sampling strip structure chart.
The specific embodiment
Below in conjunction with drawings and the specific embodiments this utility model is further described in more detail.
Embodiment one, referring to Fig. 1, and Fig. 2, Fig. 3.
The technical problems to be solved in the utility model is to overcome above-mentioned deficiency, a kind of quick survey blood uric acid instrument is provided, by adopting the potassium ferricyanide as mediator, urate oxidase is fixed on the carbon electrodes that sodium carboxymethyl cellulose was handled, be developed into a kind of uric acid biosensor.Record the electric current that chemical reaction discharges by three-electrode system, measure the content of uric acid in the detected solution indirectly.The technical solution of the utility model comprises blood-taking device, display device, and control device, control device links to each other with blood-taking device, display device.Blood-taking device comprises blood sampling strip and strip interface; Control device comprises the amplifying circuit module, action button, power module, current acquisition module, processor module and analog-to-digital conversion module; Display device is display screen.The blood sampling strip is connected with the amplifying circuit module by the strip interface, the output of amplifying circuit module is connected with the input of current acquisition module, the output of current acquisition module is connected with the input of analog-to-digital conversion module, the output of analog-to-digital conversion module is connected with the input of processor module, and the output of processor module is connected with display screen; Action button and power module are connected with processor module.
The blood sampling strip comprises dielectric base 15, be provided with working electrode 9 in dielectric base 15, reference electrode 7 and counter electrode 8, working electrode 9, reference electrode 7 and counter electrode 8 are drawn lead 6,4,5 and contact 3,1,2, cover partial electrode 9,7,8, lead 6,4,5 and part contact 3,1, insulating barrier 14 on 2, be arranged on the working electrode 9 that does not cover insulating barrier 14, reference electrode 7, reagent layer 10 on the counter electrode 8, pad 11 is pasted at edge at reagent layer 10, add a cover hydrophilic film 12 on the pad 11, by reagent layer 10, pad 11 and hydrophilic film 12 surround blood sample reaction chamber 13. Lead 6,4,5 and contact 3,1,2 be conductive material, working electrode 9, reference electrode 7, counter electrode 8 are the carbon electrodes that are printed on dielectric base 1 with the silk screen technology.Lead 6,4,5 can be the silver-colored rail that is printed on the dielectric base 1, and contact 3,1, the 2nd is printed on the carbon-coating on the dielectric base 1.Reagent layer 10 comprises bovine serum albumin, electron mediator, binding agent and PH buffer agent.Pad 11 thickness are the 70-120 micron, and the spacing of two pads 11 is 2 millimeters.The size of blood sample reaction chamber 13 has limited the sample size of blood, makes mensuration more accurate, and hydrophilic film 12 can be accelerated the suction of blood, shortens minute.Electron mediator is the potassium ferricyanide.
Take by weighing 0.006g sodium carboxymethyl cellulose (CMC), be dissolved in the 100ml water, with magnetic stirring apparatus stir treat fully dissolving after, filter with the middling speed qualitative filter paper, keep filtrate, get 10mlCMC liquid then, add the 800u urate oxidase, add 500mg K4FeCN6 again and shake up to dissolving fully, be kept under 5 degree standby in the refrigerator then.Take by weighing 80mgLI2CO3 and dissolve in the 15ml water, be dissolved to saturatedly under the room temperature, filter with qualitative filter paper then, remove slag, add the 100mg uric acid in filtrate, 50-60 degree water-bath to uric acid dissolves fully, be diluted with water to 100ml after the cooling, obtain the uric acid mother solution of 1000mg/L, 4 degree are preserved standby down.Take by weighing 12.4gNa2B407 and 4.8GKH2PO4, water dissolution shakes up and can obtain buffer (PH8.5) to 1L.Two carbon electrodes with the sensor of CMC for preparing carry out pretreatment once earlier, after waiting to dry, in a model machine (U.S., the xyz-2500 type) goes up the uricase liquid that absorption prepares, then with the reaction reagent isocenter on working electrode 9, counter electrode 8 and the reference utmost point 7, preferably at the uricase liquid of the about 0.5ul of carbon electrode each point, then, be placed on the clean place 10h that dries in the air and get final product.
This blood sampling strip can effectively be removed the interference of other compositions in the blood, can measure uric acid concentration in the whole blood rapidly and accurately, has self-priming sample introduction function, and is easy to use; And manufacture method is simple, is particularly suitable for producing in enormous quantities.Blood sampling strip of the present utility model adopts three utmost point electrical measurement systems, the electric current that forms is through the strip interface, flow to the amplifying circuit module, after the current acquisition module is gathered current signal, flow to processor module by the analog-to-digital conversion module processing, processor module carries out being presented on the display screen after set converse routine calculates concentration.Analog-to-digital conversion module can be analog-digital converter.
Control device can include real-time clock module, and the output of described processor module is connected with the input of real-time clock, and the output of real-time clock is connected with display screen.Real-time clock module can adopt the s-3530A real-time timepiece chip.This real-time clock module has the characteristics of high-precision low-power consumption, work crystal oscillator frequency 32K, and be provided with energy-saving mode, and can when the blood uric acid instrument is idle, make it enter energy-saving mode, save battery electric quantity.And calculate the leap year automatically, and represent the date time data with the binary-coded decimal form, for the read-write of processor module provides great convenience.
Control device comprises power switching module, is connected with power module and processor module.The designing power supply on-off circuit, when when shutdown can be directly by the power module power supply except processor module and real-time clock, the power supply of other modules is all cut off, make processor module and real-time clock module enter dormancy or electricity-saving state then, can save the power consumption of standby greatly, extend working time.Waking up by interrupting realizing that produce a button and interrupt when pressing the specific operation button, wake up process device module is other module energized also thus, the rearming of blood uric acid instrument of processor module.
Processor module is provided with memorizer, is used for storage of measurement data.
Processor module can be chosen Silicon labs C8051F410 single-chip microcomputer.Inner integrated abundant peripheral simulator makes the user can take full advantage of its plenty hardware resources.Utilize in the C8051F410 single-chip microcomputer 12 A/D converter to be used for doing small-signal and measure, small-signal current finally is converted to voltage by this A/D sampling through current sampling circuit, calculates concentration with set converse routine then and is presented on the display screen.Utilize 12 D/A converter can export precise and stable reference voltage for the three-electrode electro Chemical measuring process, because the output of D/A can be changed arbitrarily by programming, therefore can be very easily change pressure reduction between reference voltage and the running voltage by changing the D/A value, and accuracy guarantee that can 12 pressure reduction stable, effectively improve certainty of measurement.The Flash memorizer that inside has 32/16kB can be used for storage of measurement data.The blood uric acid instrument need be with each measurement data and dat recorder in non-volatile memory medium, usually adopt the Flash memorizer, the record data of being used for are being arranged under the situation of power supply, in each blood uric acid instrument shutdown, again data are being write among the Flash, improving the blood uric acid instrument and measure efficient.
This utility model can adopt the customization Liquid Crystal Module of a PDM1621-893 as display screen, carry out the man machine interface operation, this module can realize such as real-time clock, battery electric quantity, measurement unit, alarm signal, multiple demonstrations such as code prompting can provide abundant as far as possible information in conjunction with the programming that three seven segment numericals are shown in addition under a plurality of mode of operations.
Claims (10)
1. survey the blood uric acid instrument fast for one kind, it is characterized in that: comprise blood-taking device, display device, control device, control device links to each other with blood-taking device, display device;
Described blood-taking device comprises blood sampling strip and strip interface;
Described control device comprises the amplifying circuit module, action button, power module, current acquisition module, processor module and analog-to-digital conversion module;
Described display device is display screen;
Described blood sampling strip is connected with the amplifying circuit module by the strip interface, the output of amplifying circuit module is connected with the input of current acquisition module, the output of current acquisition module is connected with the input of analog-to-digital conversion module, the output of analog-to-digital conversion module is connected with the input of processor module, and the output of processor module is connected with display screen;
Described action button and power module are connected with processor module.
2. a kind of quick survey blood uric acid instrument according to claim 1, it is characterized in that: described blood sampling strip comprises dielectric base, be provided with working electrode, reference electrode and counter electrode at insulation base, working electrode, reference electrode and counter electrode are drawn lead and contact, cover the insulating barrier on partial electrode, lead and the part contact, be arranged on the reagent layer on the working electrode that do not cover insulating barrier, reference electrode, the counter electrode, paste pad at the edge of reagent layer and add a cover hydrophilic film, by reagent layer, pad and hydrophilic film surround blood sample reflection chamber.
3. a kind of quick survey blood uric acid instrument according to claim 2, it is characterized in that: described lead and contact are conductive material, working electrode, reference electrode, counter electrode are the carbon electrodes that is printed on dielectric base with the silk screen technology.
4. a kind of quick survey blood uric acid instrument according to claim 3, it is characterized in that: described lead is the silver-colored rail that is printed on the dielectric base, and contact is the carbon-coating that is printed on the dielectric base.
5. according to claim 2 or 3 or 4 described a kind of quick survey blood uric acid instrument, it is characterized in that: described reagent layer comprises bovine serum albumin, electron mediator, binding agent and PH buffer agent.
6. according to claim 2 or 3 or 4 described a kind of quick survey blood uric acid instrument, it is characterized in that: described spacer thickness is the 70-120 micron, and the spacing of two pads is 2 millimeters.
7. a kind of quick survey blood uric acid instrument according to claim 5, it is characterized in that: described electron mediator is the potassium ferricyanide.
8. a kind of quick survey blood uric acid instrument according to claim 1 is characterized in that described control device comprises real-time clock module, and the output of described processor module is connected with the input of real-time clock, and the output of real-time clock is connected with display screen.
9. according to claim 1 or 8 described a kind of quick survey blood uric acid instrument, it is characterized in that: described control device comprises power switching module, is connected with power module and processor module.
10. according to claim 1 or 8 described a kind of quick survey blood uric acid instrument, it is characterized in that: described processor module is provided with memorizer, is used for storage of measurement data.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320081103 CN203195700U (en) | 2013-02-22 | 2013-02-22 | Fast blood uric acid measuring instrument |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320081103 CN203195700U (en) | 2013-02-22 | 2013-02-22 | Fast blood uric acid measuring instrument |
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| CN203195700U true CN203195700U (en) | 2013-09-18 |
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| CN 201320081103 Expired - Fee Related CN203195700U (en) | 2013-02-22 | 2013-02-22 | Fast blood uric acid measuring instrument |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104000601A (en) * | 2013-02-22 | 2014-08-27 | 谭唱 | Rapid blood uric acid measuring instrument |
| CN105116017A (en) * | 2015-09-08 | 2015-12-02 | 无锡百灵传感技术有限公司 | Chip type integrated sensor |
| CN115684317A (en) * | 2022-10-28 | 2023-02-03 | 东莞市源凯电气有限公司 | Purine detection module and method |
-
2013
- 2013-02-22 CN CN 201320081103 patent/CN203195700U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104000601A (en) * | 2013-02-22 | 2014-08-27 | 谭唱 | Rapid blood uric acid measuring instrument |
| CN105116017A (en) * | 2015-09-08 | 2015-12-02 | 无锡百灵传感技术有限公司 | Chip type integrated sensor |
| CN115684317A (en) * | 2022-10-28 | 2023-02-03 | 东莞市源凯电气有限公司 | Purine detection module and method |
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Granted publication date: 20130918 Termination date: 20180222 |
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| CF01 | Termination of patent right due to non-payment of annual fee |