CN115684317A - Purine detection module and method - Google Patents
Purine detection module and method Download PDFInfo
- Publication number
- CN115684317A CN115684317A CN202211334419.1A CN202211334419A CN115684317A CN 115684317 A CN115684317 A CN 115684317A CN 202211334419 A CN202211334419 A CN 202211334419A CN 115684317 A CN115684317 A CN 115684317A
- Authority
- CN
- China
- Prior art keywords
- module
- purine
- data processing
- data
- processing module
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- KDCGOANMDULRCW-UHFFFAOYSA-N 7H-purine Chemical compound N1=CNC2=NC=NC2=C1 KDCGOANMDULRCW-UHFFFAOYSA-N 0.000 title claims abstract description 154
- 238000001514 detection method Methods 0.000 title claims abstract description 38
- 238000000034 method Methods 0.000 title claims abstract description 14
- 238000012545 processing Methods 0.000 claims abstract description 49
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 19
- 230000003321 amplification Effects 0.000 claims abstract description 17
- 238000003199 nucleic acid amplification method Methods 0.000 claims abstract description 17
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 11
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 11
- 239000002002 slurry Substances 0.000 claims abstract description 6
- 102000004190 Enzymes Human genes 0.000 claims abstract description 5
- 108090000790 Enzymes Proteins 0.000 claims abstract description 5
- 238000004891 communication Methods 0.000 claims description 9
- 238000006243 chemical reaction Methods 0.000 claims description 7
- 239000007788 liquid Substances 0.000 claims description 7
- 230000008569 process Effects 0.000 claims description 4
- 239000002390 adhesive tape Substances 0.000 claims description 2
- 238000004458 analytical method Methods 0.000 claims description 2
- 239000010410 layer Substances 0.000 abstract description 30
- 239000012790 adhesive layer Substances 0.000 abstract description 2
- 239000000758 substrate Substances 0.000 abstract description 2
- 235000013305 food Nutrition 0.000 description 6
- FDGQSTZJBFJUBT-UHFFFAOYSA-N hypoxanthine Chemical compound O=C1NC=NC2=C1NC=N2 FDGQSTZJBFJUBT-UHFFFAOYSA-N 0.000 description 6
- 238000012360 testing method Methods 0.000 description 4
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 3
- UGQMRVRMYYASKQ-UHFFFAOYSA-N Hypoxanthine nucleoside Natural products OC1C(O)C(CO)OC1N1C(NC=NC2=O)=C2N=C1 UGQMRVRMYYASKQ-UHFFFAOYSA-N 0.000 description 3
- 108010093894 Xanthine oxidase Proteins 0.000 description 3
- 102100033220 Xanthine oxidase Human genes 0.000 description 3
- 239000007800 oxidant agent Substances 0.000 description 3
- 201000005569 Gout Diseases 0.000 description 2
- 102000003992 Peroxidases Human genes 0.000 description 2
- 208000030159 metabolic disease Diseases 0.000 description 2
- 230000001590 oxidative effect Effects 0.000 description 2
- 108040007629 peroxidase activity proteins Proteins 0.000 description 2
- 230000027756 respiratory electron transport chain Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 230000000007 visual effect Effects 0.000 description 2
- 201000001431 Hyperuricemia Diseases 0.000 description 1
- LEHOTFFKMJEONL-UHFFFAOYSA-N Uric Acid Chemical compound N1C(=O)NC(=O)C2=C1NC(=O)N2 LEHOTFFKMJEONL-UHFFFAOYSA-N 0.000 description 1
- TVWHNULVHGKJHS-UHFFFAOYSA-N Uric acid Natural products N1C(=O)NC(=O)C2NC(=O)NC21 TVWHNULVHGKJHS-UHFFFAOYSA-N 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 208000026816 acute arthritis Diseases 0.000 description 1
- 206010003246 arthritis Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 238000013500 data storage Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000006479 redox reaction Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 235000014102 seafood Nutrition 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 229940116269 uric acid Drugs 0.000 description 1
Images
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a purine detection module and a method, which comprises a detection reagent strip, a control circuit module and a display module, wherein the detection reagent strip comprises a substrate layer, a wire layer, a carbon slurry layer, an insulating layer, a double-sided adhesive layer and a hydrophilic film layer which are sequentially stacked, the control circuit module comprises a signal amplification module, an AD acquisition module, a data processing module and a power supply module, the wire layer is connected with the AD acquisition module through the signal amplification module, and the AD acquisition module, the power supply module and the display module are all connected with the data processing module. According to the invention, when the enzyme on the detection reagent strip reacts with purine, a micro-current signal is generated on the lead layer, the micro-current signal is amplified by the signal amplification circuit and is subjected to data acquisition by the AD acquisition module and then is processed by the data processing module, so that the current signal is converted into the concentration value of purine, and the purine concentration in the object to be detected is rapidly, stably, conveniently, sensitively and visually tested.
Description
Technical Field
The invention relates to the technical field of purine detection, in particular to a purine detection module and a method.
Background
In recent years, the global incidence of gout has been on the rise. Wherein, if high purine food such as seafood, meat and other foods are taken for a long time, the purine is easily produced in the human body to cause purine metabolic disorder, and the purine substance in the human body can produce gout after long-term metabolic disorder, and the clinical characteristics of the food are hyperuricemia, repeated attack of acute arthritis, formation of tophus, joint deformity, kidney parenchymal lesion and the like. In order to control the purine intake in the food of a patient, the purine content of the food needs to be rapidly detected to determine whether the food is suitable for consumption.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provides a purine detection module and a method.
The technical scheme of the invention is as follows:
the utility model provides a purine detection module, includes detection reagent strip, control circuit module and display module, detection reagent strip is including superimposed base plate layer, wire layer, carbon thick liquid layer, insulating layer, double faced adhesive tape layer and hydrophilic rete in proper order, control circuit module includes signal amplification module, AD acquisition module, data processing module and power module, the wire layer passes through signal amplification module with AD acquisition module connects, AD acquisition module power module display module all with data processing module connects, the little current signal warp that produces on the wire layer after carbon thick liquid layer and purine reaction signal amplification module enlargies back and turns into current data, AD acquisition module sends this current data to data processing module, data processing module obtains purine data and is in after carrying out the analysis to current data display module shows.
Preferably, the control circuit module further includes a storage module, and the storage module is connected to the data processing module and is configured to store the purine data analyzed and processed by the data processing module.
Preferably, the control circuit module further comprises a communication module, and the communication module is connected with the data processing module and is used for sending the purine data to the server or receiving an instruction of the server.
Preferably, the control circuit module further includes a key control circuit, and the key control circuit is connected to the data processing module and is configured to input a control signal.
Preferably, the signal amplification module, the AD acquisition module, the data processing module and the power supply module are integrated on a circuit board.
A method for detecting a purine, comprising the steps of:
1) Immersing a detection reagent strip into a liquid containing purine, wherein the enzyme in a carbon slurry layer in the detection reagent strip generates a chemical reaction with the purine and generates a micro-current signal in a lead layer in the detection reagent strip;
2) The micro-current signal is amplified by a signal amplifying circuit and converted into current data;
3) The AD acquisition circuit acquires current data and sends the current data to the data processing module;
4) The data processing module analyzes and processes the current data to obtain purine data, and the purine data is displayed on the display module.
Preferably, the data processing module stores the purine data in the storage module.
Preferably, the data processing module sends the purine data to the server or receives an instruction of the server.
Preferably, the control signal is input to the data processing module through the key control circuit.
Compared with the prior art, the invention has the beneficial effects that: according to the invention, when the enzyme on the detection reagent strip reacts with purine, a micro-current signal is generated on the lead layer, the micro-current signal is amplified by the signal amplification circuit and is subjected to data acquisition by the AD acquisition module and then is processed by the data processing module, so that the current signal is converted into the concentration value of purine, and the purine concentration in the object to be detected is rapidly, stably, conveniently, sensitively and visually tested.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed for the embodiments or the prior art descriptions will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.
FIG. 1 is a schematic block diagram of a purine detection module according to the present invention;
FIG. 2 is a schematic view of the test strip of the present invention;
FIG. 3 is an exploded view of the test strip of the present invention;
FIG. 4 is a flow chart of the purine detection method of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
In order to illustrate the technical means of the present invention, the following description is given by way of specific examples.
Example 1
As shown in fig. 1 to 3, the purine detection module of this embodiment includes a detection reagent strip 1, a control circuit module 2, and a display module 3, where the detection reagent strip 1 includes a substrate layer 11, a wire layer 12, a carbon slurry layer 13, an insulating layer 14, a double-sided adhesive layer 15, and a hydrophilic film layer 16, which are sequentially stacked, the control circuit module 2 includes a signal amplification module 21, an AD acquisition (data acquisition) module 22, a data processing module 23, and a power module 24, the wire layer 12 is connected to the AD acquisition module 22 through the signal amplification module 21, the AD acquisition module 22, the power module 24, and the display module 3 are all connected to the data processing module 23, a micro-current signal generated on the wire layer 12 after the reaction between the carbon slurry layer 13 and purine is amplified by the signal amplification module 21 and converted into current data, the AD acquisition module 22 sends the current data to the data processing module 23, and the data processing module 23 analyzes the current data to obtain purine data and displays the purine data on the display module 3. The carbon pulp layer 13 contains an oxidizing agent and xanthine oxidase, and the like, and mainly promotes purine to chemically react. The chemical reaction for purine production is as follows:
purine + oxidant → hypoxanthine
Purine substances are oxidized into hypoxanthine through an oxidizing agent, the hypoxanthine is catalyzed by XOD (xanthine oxidase) to generate uric acid and hydrogen peroxide, the generated H2O2 and a reducing electron mediator are subjected to redox reaction under the action of POD (peroxidase) to generate the oxidizing electron mediator, electron transfer occurs in the process, and a constant voltage is applied between a cathode and an anode on a lead layer, so that the electron transfer is converted into current between the two electrodes. The current has a certain proportional relation with the purine concentration, and the data processing module obtains the purine concentration. The signal amplification module 21, the AD acquisition module 22, the data processing module 23, and the power supply module 24 are commonly used components, the electrical connection relationship among them is commonly used circuit connection, and the data processing module 23 is commonly used CPU with data analysis function. The purine detection module of the embodiment converts the current signal into the concentration value of purine, thereby realizing the rapid, stable, convenient, sensitive and visual test of the purine concentration in the object to be tested.
In this embodiment, the control circuit 2 further includes a storage module 25, and the storage module 25 is connected to the data processing module 23 and is configured to store purine data analyzed and processed by the data processing module 23; the control circuit module 2 further comprises a communication module 26, and the communication module 26 is connected with the data processing module 23 and is used for sending purine data to a server or receiving an instruction of the server; the control circuit module 2 further includes a key control circuit 27, and the key control circuit 27 is connected to the data processing module 23 and configured to input an operation signal. The above components are common components, the electrical connection relationship between the components adopts common circuit connection, and the whole purine detection module has the functions of data storage, remote communication, key regulation and control and the like by using the storage module 25, the communication module 26 and the key control circuit 27. The signal amplification module 21, the AD acquisition module 22, the data processing module 23, the power module 24, the storage module 25, the communication module 26, the key control circuit 27, the display module 3 and the like are integrated on one circuit board, so that the whole structure is more integrated.
Example 2
As shown in fig. 1 to 4, the purine detection method of the present embodiment, based on embodiment 1, includes the following steps:
1) Immersing a detection reagent strip into a liquid containing purine, wherein the enzyme in a carbon slurry layer in the detection reagent strip generates a chemical reaction with the purine and generates a micro-current signal in a lead layer in the detection reagent strip;
2) The micro-current signal is amplified by a signal amplifying circuit and converted into current data;
3) The AD acquisition circuit acquires current data and sends the current data to the data processing module;
4) The data processing module analyzes and processes the current data to obtain purine data, and the purine data is displayed on the display module.
The purine detection method of the embodiment converts the current signal into the concentration value of purine, thereby realizing the rapid, stable, convenient, sensitive and visual test of the purine concentration in the object to be tested.
In the above step, the data processing module stores the purine data in the storage module; the data processing module sends purine data to a server or receives an instruction of the server; control signals are input to the data processing module through the key control circuit, and data are conveniently stored, remotely exchanged and key-pressed to be regulated and controlled.
The present invention is not limited to the above preferred embodiments, and any modifications, equivalent substitutions and improvements made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (9)
1. The utility model provides a purine detects module which characterized in that: including detection reagent strip, control circuit module and display module, detection reagent strip is including superimposed base plate layer, wire layer, carbon thick liquid layer, insulating layer, double faced adhesive tape layer and hydrophilic rete in proper order, control circuit module includes signal amplification module, AD acquisition module, data processing module and power module, the wire layer passes through signal amplification module with AD acquisition module connects, AD acquisition module power module display module all with data processing module connects, the little current signal warp that produces on the wire layer after carbon thick liquid layer and purine reaction signal amplification module enlargies back and turns into current data, AD acquisition module sends this current data to data processing module, data processing module obtains purine data and is in after carrying out the analysis to current data display module shows.
2. The purine detection module of claim 1, wherein: the control circuit module further comprises a storage module, and the storage module is connected with the data processing module and used for storing purine data analyzed and processed by the data processing module.
3. The purine detection module of claim 1, wherein: the control circuit module further comprises a communication module, and the communication module is connected with the data processing module and used for sending purine data to a server or receiving an instruction of the server.
4. The purine detection module of claim 1, wherein: the control circuit module further comprises a key control circuit, and the key control circuit is connected with the data processing module and used for inputting control signals.
5. The purine detection module of claim 1, wherein: the signal amplification module, the AD acquisition module, the data processing module and the power module are integrated on a circuit board.
6. A purine detection method, comprising: the method comprises the following steps:
1) Immersing the detection reagent strip into liquid containing purine, wherein the enzyme in the carbon slurry layer in the detection reagent strip and the purine generate chemical reaction and generate a micro-current signal in the lead layer in the detection reagent strip;
2) The micro-current signal is amplified by a signal amplifying circuit and converted into current data;
3) The AD acquisition circuit acquires current data and sends the current data to the data processing module;
4) The data processing module analyzes and processes the current data to obtain purine data and displays the purine data on the display module.
7. The method for detecting a purine according to claim 6, wherein: the data processing module stores the purine data in the storage module.
8. The method for detecting a purine according to claim 6, wherein: and the data processing module sends the purine data to the server or receives an instruction of the server.
9. The method for detecting a purine according to claim 6, wherein: and the control signal is input to the data processing module through the key control circuit.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211334419.1A CN115684317A (en) | 2022-10-28 | 2022-10-28 | Purine detection module and method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211334419.1A CN115684317A (en) | 2022-10-28 | 2022-10-28 | Purine detection module and method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115684317A true CN115684317A (en) | 2023-02-03 |
Family
ID=85045937
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211334419.1A Pending CN115684317A (en) | 2022-10-28 | 2022-10-28 | Purine detection module and method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115684317A (en) |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH02157646A (en) * | 1988-12-09 | 1990-06-18 | Matsushita Electric Ind Co Ltd | Biosensor |
| JPH05196596A (en) * | 1991-10-21 | 1993-08-06 | Matsushita Electric Ind Co Ltd | Biosensor |
| CN1262739A (en) * | 1998-04-02 | 2000-08-09 | 松下电器产业株式会社 | Substrate determining method |
| CN1851459A (en) * | 2005-04-22 | 2006-10-25 | 清华大学 | Multi-parameter whole blood integrating test bar |
| CN1952653A (en) * | 2006-10-01 | 2007-04-25 | 中南大学 | Disposable whole blood uric acid testing electrode indicator bar and manufacturing method thereof |
| CN102483400A (en) * | 2009-09-04 | 2012-05-30 | 生命扫描苏格兰有限公司 | Methods, system and device to identify a type of test strip |
| CN102507670A (en) * | 2011-09-30 | 2012-06-20 | 长沙三诺生物传感技术股份有限公司 | Electrochemical uric acid test strip and manufacturing method thereof |
| CN203195700U (en) * | 2013-02-22 | 2013-09-18 | 谭唱 | Fast blood uric acid measuring instrument |
| CN107202826A (en) * | 2016-03-16 | 2017-09-26 | 爱科来株式会社 | Use the substance-measuring method and measure device of electric chemical formula biology sensor |
| CN113567522A (en) * | 2021-08-25 | 2021-10-29 | 上海微创生命科技有限公司 | Biosensor and preparation method thereof |
-
2022
- 2022-10-28 CN CN202211334419.1A patent/CN115684317A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH02157646A (en) * | 1988-12-09 | 1990-06-18 | Matsushita Electric Ind Co Ltd | Biosensor |
| JPH05196596A (en) * | 1991-10-21 | 1993-08-06 | Matsushita Electric Ind Co Ltd | Biosensor |
| CN1262739A (en) * | 1998-04-02 | 2000-08-09 | 松下电器产业株式会社 | Substrate determining method |
| CN1851459A (en) * | 2005-04-22 | 2006-10-25 | 清华大学 | Multi-parameter whole blood integrating test bar |
| CN1952653A (en) * | 2006-10-01 | 2007-04-25 | 中南大学 | Disposable whole blood uric acid testing electrode indicator bar and manufacturing method thereof |
| CN102483400A (en) * | 2009-09-04 | 2012-05-30 | 生命扫描苏格兰有限公司 | Methods, system and device to identify a type of test strip |
| CN102507670A (en) * | 2011-09-30 | 2012-06-20 | 长沙三诺生物传感技术股份有限公司 | Electrochemical uric acid test strip and manufacturing method thereof |
| CN203195700U (en) * | 2013-02-22 | 2013-09-18 | 谭唱 | Fast blood uric acid measuring instrument |
| CN107202826A (en) * | 2016-03-16 | 2017-09-26 | 爱科来株式会社 | Use the substance-measuring method and measure device of electric chemical formula biology sensor |
| CN113567522A (en) * | 2021-08-25 | 2021-10-29 | 上海微创生命科技有限公司 | Biosensor and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US5656142A (en) | Biosensor for measuring gas and the manufacturing method thereof | |
| Kakehi et al. | A novel wireless glucose sensor employing direct electron transfer principle based enzyme fuel cell | |
| US5565085A (en) | Method for quantifying specific compound | |
| Prévoteau et al. | Oxygen reduction on redox mediators may affect glucose biosensors based on “wired” enzymes | |
| CA2170660C (en) | Method and apparatus for reduction of bias in amperometric sensors | |
| Palleschi et al. | Amperometric tetrathiafulvalene-mediated lactate electrode using lactate oxidase absorbed on carbon foil | |
| Zhang et al. | ZnS quantum dots derived a reagentless uric acid biosensor | |
| Abass et al. | Development of an amperometric sulfite biosensor based on sulfite oxidase with cytochrome c, as electron acceptor, and a screen-printed transducer | |
| JPH09500450A (en) | Method and device for electrochemical measurement | |
| AU1022697A (en) | Device for monitoring changes in analyte concentration | |
| RU2660316C2 (en) | Analytical test strip with integrated battery | |
| Du et al. | A glucose biosensor based on electrocatalytic oxidation of NADPH at single-walled carbon nanotubes functionalized with poly (nile blue A) | |
| Bilitewski et al. | Glucose biosensors based on thick film technology | |
| CN108982617A (en) | A kind of uric acid electrochemical test strip and preparation method thereof | |
| CN115684317A (en) | Purine detection module and method | |
| CN115266865A (en) | Method for improving stability of electrochemical sensor | |
| Ikeda et al. | Amperometric response to reducing carbohydrates of an enzyme electrode based on oligosaccharide dehydrogenase. Detection of lactose and α-amylase | |
| Davis et al. | Detection of ATP and creatine kinase using an enzyme electrode | |
| TW202221317A (en) | Biochemical test chip | |
| CN115684298A (en) | Purine detection kit | |
| CN101393158B (en) | Biology sensing enzyme detection electrode for hydrogen peroxide and detection method thereof | |
| CN107703203B (en) | Heavy metal detection device and detection method thereof | |
| US20130220837A1 (en) | Systems and methods for carbohydrate detection | |
| TW201945728A (en) | Glucose sensor | |
| EP1456638A1 (en) | System and method for the determination of dissolved oxygen in a fluid |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |