CN1952653B - Disposable whole blood uric acid testing electrode indicator bar and manufacturing method thereof - Google Patents
Disposable whole blood uric acid testing electrode indicator bar and manufacturing method thereof Download PDFInfo
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- CN1952653B CN1952653B CN2006100323821A CN200610032382A CN1952653B CN 1952653 B CN1952653 B CN 1952653B CN 2006100323821 A CN2006100323821 A CN 2006100323821A CN 200610032382 A CN200610032382 A CN 200610032382A CN 1952653 B CN1952653 B CN 1952653B
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- 210000004369 blood Anatomy 0.000 title claims abstract description 31
- 239000008280 blood Substances 0.000 title claims abstract description 31
- LEHOTFFKMJEONL-UHFFFAOYSA-N Uric Acid Chemical compound N1C(=O)NC(=O)C2=C1NC(=O)N2 LEHOTFFKMJEONL-UHFFFAOYSA-N 0.000 title claims abstract description 29
- TVWHNULVHGKJHS-UHFFFAOYSA-N Uric acid Natural products N1C(=O)NC(=O)C2NC(=O)NC21 TVWHNULVHGKJHS-UHFFFAOYSA-N 0.000 title claims abstract description 29
- 229940116269 uric acid Drugs 0.000 title claims abstract description 29
- 238000012360 testing method Methods 0.000 title claims description 11
- 238000004519 manufacturing process Methods 0.000 title abstract description 8
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 25
- 108091003079 Bovine Serum Albumin Proteins 0.000 claims abstract description 4
- 229940098773 bovine serum albumin Drugs 0.000 claims abstract description 4
- -1 potassium ferricyanide Chemical compound 0.000 claims description 6
- 238000009413 insulation Methods 0.000 claims description 5
- 239000004372 Polyvinyl alcohol Substances 0.000 claims description 4
- 239000001768 carboxy methyl cellulose Substances 0.000 claims description 4
- 239000003795 chemical substances by application Substances 0.000 claims description 4
- 239000006179 pH buffering agent Substances 0.000 claims description 4
- 229920002451 polyvinyl alcohol Polymers 0.000 claims description 4
- DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 claims description 3
- 235000019812 sodium carboxymethyl cellulose Nutrition 0.000 claims description 3
- 229920001027 sodium carboxymethylcellulose Polymers 0.000 claims description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 claims description 2
- 229920000151 polyglycol Polymers 0.000 claims description 2
- 239000010695 polyglycol Substances 0.000 claims description 2
- 235000019422 polyvinyl alcohol Nutrition 0.000 claims description 2
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- 229920000036 polyvinylpyrrolidone Polymers 0.000 claims description 2
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 claims description 2
- 150000001642 boronic acid derivatives Chemical group 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 18
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- 239000011248 coating agent Substances 0.000 abstract description 2
- 238000000576 coating method Methods 0.000 abstract description 2
- 238000005070 sampling Methods 0.000 abstract 3
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- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 7
- 229910052799 carbon Inorganic materials 0.000 description 7
- 239000002585 base Substances 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 5
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 4
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- 239000007924 injection Substances 0.000 description 4
- 238000002347 injection Methods 0.000 description 4
- 239000000758 substrate Substances 0.000 description 4
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 3
- 238000000835 electrochemical detection Methods 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 229920000139 polyethylene terephthalate Polymers 0.000 description 3
- 239000005020 polyethylene terephthalate Substances 0.000 description 3
- KDCGOANMDULRCW-UHFFFAOYSA-N 7H-purine Chemical compound N1=CNC2=NC=NC2=C1 KDCGOANMDULRCW-UHFFFAOYSA-N 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 235000010323 ascorbic acid Nutrition 0.000 description 2
- 229960005070 ascorbic acid Drugs 0.000 description 2
- 239000011668 ascorbic acid Substances 0.000 description 2
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- VYFYYTLLBUKUHU-UHFFFAOYSA-N dopamine Chemical compound NCCC1=CC=C(O)C(O)=C1 VYFYYTLLBUKUHU-UHFFFAOYSA-N 0.000 description 2
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 2
- FDGQSTZJBFJUBT-UHFFFAOYSA-N hypoxanthine Chemical compound O=C1NC=NC2=C1NC=N2 FDGQSTZJBFJUBT-UHFFFAOYSA-N 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- IYDGMDWEHDFVQI-UHFFFAOYSA-N phosphoric acid;trioxotungsten Chemical compound O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.OP(O)(O)=O IYDGMDWEHDFVQI-UHFFFAOYSA-N 0.000 description 2
- 239000002504 physiological saline solution Substances 0.000 description 2
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 2
- 229910052709 silver Inorganic materials 0.000 description 2
- 239000004332 silver Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 238000004448 titration Methods 0.000 description 2
- 229920002799 BoPET Polymers 0.000 description 1
- BTBUEUYNUDRHOZ-UHFFFAOYSA-N Borate Chemical compound [O-]B([O-])[O-] BTBUEUYNUDRHOZ-UHFFFAOYSA-N 0.000 description 1
- 229920002134 Carboxymethyl cellulose Polymers 0.000 description 1
- 208000027219 Deficiency disease Diseases 0.000 description 1
- 102100038415 ELKS/Rab6-interacting/CAST family member 1 Human genes 0.000 description 1
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- 208000002972 Hepatolenticular Degeneration Diseases 0.000 description 1
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- 208000010747 Hodgkins lymphoma Diseases 0.000 description 1
- 101001100208 Homo sapiens ELKS/Rab6-interacting/CAST family member 1 Proteins 0.000 description 1
- 101000876444 Homo sapiens ERC protein 2 Proteins 0.000 description 1
- 208000031226 Hyperlipidaemia Diseases 0.000 description 1
- UGQMRVRMYYASKQ-UHFFFAOYSA-N Hypoxanthine nucleoside Natural products OC1C(O)C(CO)OC1N1C(NC=NC2=O)=C2N=C1 UGQMRVRMYYASKQ-UHFFFAOYSA-N 0.000 description 1
- 239000005041 Mylar™ Substances 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 102000011931 Nucleoproteins Human genes 0.000 description 1
- 108010061100 Nucleoproteins Proteins 0.000 description 1
- 201000004681 Psoriasis Diseases 0.000 description 1
- PYMYPHUHKUWMLA-LMVFSUKVSA-N Ribose Natural products OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-LMVFSUKVSA-N 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 102000004357 Transferases Human genes 0.000 description 1
- 108090000992 Transferases Proteins 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 206010047700 Vomiting Diseases 0.000 description 1
- 208000018839 Wilson disease Diseases 0.000 description 1
- 108010093894 Xanthine oxidase Proteins 0.000 description 1
- 102100033220 Xanthine oxidase Human genes 0.000 description 1
- 208000017733 acquired polycythemia vera Diseases 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- HMFHBZSHGGEWLO-UHFFFAOYSA-N alpha-D-Furanose-Ribose Natural products OCC1OC(O)C(O)C1O HMFHBZSHGGEWLO-UHFFFAOYSA-N 0.000 description 1
- 238000004159 blood analysis Methods 0.000 description 1
- 229910021538 borax Inorganic materials 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 235000010948 carboxy methyl cellulose Nutrition 0.000 description 1
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- 208000002925 dental caries Diseases 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 229940111685 dibasic potassium phosphate Drugs 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 229960003638 dopamine Drugs 0.000 description 1
- 238000003487 electrochemical reaction Methods 0.000 description 1
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- 239000012467 final product Substances 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
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- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 239000002858 neurotransmitter agent Substances 0.000 description 1
- 239000012476 oxidizable substance Substances 0.000 description 1
- 230000000505 pernicious effect Effects 0.000 description 1
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 1
- 238000005375 photometry Methods 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
- 229920000515 polycarbonate Polymers 0.000 description 1
- 239000004417 polycarbonate Substances 0.000 description 1
- 208000037244 polycythemia vera Diseases 0.000 description 1
- 229920006267 polyester film Polymers 0.000 description 1
- 239000004800 polyvinyl chloride Substances 0.000 description 1
- 229920000915 polyvinyl chloride Polymers 0.000 description 1
- 239000000276 potassium ferrocyanide Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000004144 purine metabolism Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 239000004328 sodium tetraborate Substances 0.000 description 1
- 235000010339 sodium tetraborate Nutrition 0.000 description 1
- XOGGUFAVLNCTRS-UHFFFAOYSA-N tetrapotassium;iron(2+);hexacyanide Chemical compound [K+].[K+].[K+].[K+].[Fe+2].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-] XOGGUFAVLNCTRS-UHFFFAOYSA-N 0.000 description 1
- 238000002054 transplantation Methods 0.000 description 1
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Images
Abstract
The invention relates to a one-time electrode detector for detecting uric acid in whole blood and its method of manufacture. The detector comprises insulated base (1), reference electrode (2), working electrode (3), reagent layer (4), sampling cavity (5). The reference electrode (2) and the working electrode (3) are carbon-coating which is silk-screened on the base (1); the reagent layer (4) consists of bovine serum albumin, electronic agency, agglomerant and pH buffer; the sampling cavity (5) is rounded by the reagent layer (4), shim (6) and hydrophilic film (7). The detector can effectively remove the interference of other compositions in blood and quickly and accurately detect the density of uric acid in whole blood; has the function of self-priming and automatic sampling, it is convenient to use; the method for making is simple, it is fit for mass production.
Description
Technical field
The present invention relates to a kind of disposable whole blood uric acid testing electrode indicator bar and manufacture method of utilizing electrochemical process to detect uric acid concentration in the blood.
Background technology
Uric acid (2,4, the 6-trihydroxypurine is called for short UA) is the final product of purine metabolism in the human body, does not have physiological function in human body.The main source of purine has diet, nucleoprotein to decompose and from the beginning synthetic three kinds of approach in the human body.Under the normal condition, mainly by renal excretion, all the other get rid of external through enteron aisle, skin, hair etc.Gout, hyperlipidemia, malignant hematologic disease, psoriasis, kidney disease, chronic leukemia, Huppert's disease, unicellular increase disease, polycythemia veras etc. all can reflect uric acid content clinically and raise, and the xanthine oxidase vigor reduces, Wilson's disease, hypoxanthine guanine ribose transferase deficiency disease, metabolic acidaminuria and Hodgkin's disease etc. can show as uric acid clinically and reduce, therefore can be used as the important indicator of the above illness of diagnosis, and the blood uric acid content is also to pregnant pernicious vomiting, antenatal, postpartum, the chemotherapy of various tumours, behind the renal transplantation, and the observation of the renal function of Extracorporeal Circulation, important clinical significance is all arranged.
The method of the mensuration uric acid of having reported at present mainly can be divided into gravimetric method, titrimetry, reducing process and enzyme process etc.Gravimetric method and titrimetry complex operation, and accuracy is low, is unsuitable for the accurate mensuration of uric acid; Usually adopt reducing process and enzymic colorimetric clinically.In the reducing process, uric acid can be oxidized to urea by phosphotungstic acid under the alkali condition again, and phosphotungstic acid is reduced into tungsten blue, and latter's growing amount is directly proportional with uric acid content.Its shortcoming is: the ascorbic acid in the blood sample easily disturbs; Sample must take off albumen in advance to be handled; Need expensive device and specialty analysis personnel.Enzyme process is divided into enzyme connection photometry and enzyme connection electrochemical process, the former is usually used in the automatic biochemistry analyzer of hospital, is suitable for the analysis operation of sample in enormous quantities, but is serum or blood plasma through centrifuging before the blood analysis, require professional operating personnel and special reagent, be unsuitable for using in the family.
Summary of the invention
Detect the technical matters that exists for solving above-mentioned uric acid, the invention provides a kind of disposable whole blood uric acid testing electrode indicator bar and the manufacture method that can conveniently measure the blood uric acid content in various occasions fast.This disposable whole blood uric acid testing electrode indicator bar only needs the blood of trace, can accurately measure the content of blood uric acid at short notice.
The technical scheme that the present invention solves the problems of the technologies described above is: comprise a dielectric base, on dielectric base, be provided with working electrode and contrast electrode, and working electrode and contrast electrode draw lead and contact, cover the insulation course on partial electrode, lead and the part contact, be arranged at the reagent layer on working electrode and the contrast electrode, pad is pasted at edge at reagent layer, and hydrophilic film is added a cover on top, surrounds sample cavity by reagent layer, pad and hydrophilic film.
The manufacture method of disposable whole blood uric acid testing electrode indicator bar may further comprise the steps:
1), on dielectric base, print working electrode and contrast electrode, and working electrode and contrast electrode draw lead and contact;
2), insulation course is set drawing on lead, part working electrode and contrast electrode and the part contact;
3), on working electrode and contrast electrode, point out reagent layer with point sample instrument;
4), paste pad at the edge of reagent layer, top is added a cover hydrophilic film and is formed sample cavity and injection port.
Advantage of the present invention: can eliminate a large amount of readily oxidizable substances that exist in the blood when 1) detecting blood sugar, improve accuracy and sensitivity; 2) detect current potential 400mV, eliminated the interference of ascorbic acid and neurotransmitter such as dopamine; 3) the present invention only needs micro blood can measure the content of blood uric acid within 10 seconds, can reduce patient and go the inconvenience of hospital and reduce the patient medical spending.
The present invention is further illustrated for following structure the drawings and specific embodiments.
Description of drawings
Fig. 1 is the structural representation of invention, and wherein 1a is a vertical view, and 1b is the cut-open view figure of 1a.
Fig. 2 (2a-2e) is a process drawing of the present invention.
Fig. 3 is the graph of a relation of voltage of the present invention and uric acid concentration.
Fig. 4 measures the comparison of unknown blood sample result with large-scale Biochemical Analyzer Hitach7600 measurement result for the present invention.
Embodiment
Referring to Fig. 1 and Fig. 2, the present invention prints working electrode 2 and contrast electrode 3 on a dielectric base 1, and working electrode 2 and contrast electrode 3 draw lead (8,9) and contact (10,11), cover partial electrode (2,3), lead (8,9) and part contact (10,11) insulation course 12 on is arranged at the reagent layer 4 on working electrode 2 and the contrast electrode 3, pastes pad 6 at the edge of reagent layer 4, hydrophilic film 7 is added a cover on top, surrounds sample cavity 5 by reagent layer 4, pad 6 and hydrophilic film 7.
Among the present invention, the substrate 1 that is used to make strip is for any non-conductive, the material of stable in properties.Suitable material has mylar, Polyvinylchloride and polycarbonate; Substrate, any conductive material can be used in lead and contact, comprise silver, gold, platinum, carbon, the preferred conductive carbon of working electrode and contrast electrode part, model has Carbon Electrode 423 (Acheson Colloids) and ERC1, ERC2 (Ercon Inc.), and working electrode 2 and contrast electrode 3 are to be printed on carbon electrode on the dielectric base 1 with the silk screen technology, electrode draw lead (8,9) be the silver wire that is printed on the dielectric base 1, joint (10,11) is to be printed on suprabasil carbon-coating.With a model machine reaction reagent isocenter is added on working electrode 2 and the contrast electrode 4, forms reagent layer 4.Reagent layer 4 comprises bovine serum albumin(BSA), electron mediator, cementing agent and pH buffering agent.Electron mediator is the potassium ferricyanide; Cementing agent is any of sodium carboxymethyl cellulose, polyvinylpyrrolidone, polyvinyl alcohol (PVA) or polyglycol; The pH buffering agent is any of dipotassium hydrogen phosphate, potassium dihydrogen phosphate, borate, citrate, and its pH value is 8.5.Reagent layer 4, pad 6 and hydrophilic film 7 surround sample cavity 5, and pad 6 thickness are the 70-120 micron, and the spacing of two pads is 2 millimeters, and injection port or exhausr port are enclosed in the two ends of sample cavity, and cavity wide (spacings of two pads) is 2 millimeters; The size of sample cavity has limited the sample size of blood, makes mensuration more accurate; Hydrophilic film can be accelerated the suction of blood, shortens minute.
The manufacture method of this disposable whole blood uric acid testing electrode indicator bar is as follows: the thick PET polyester film (polyethylene terephthalate of substrate 1 preferred 500 μ m, Polyethylene terephthalate, Yangzhou Jinfeng New Material Co., Ltd.), the first step is a silk- screen silver rail 4,5 in substrate, at silver-colored rail 4,5 end conductive carbon (Carbon Electrode 423, Acheson Colloids Co.) goes out carbon pad 2,3 by the silk screen technology print, then with silk screen technology print insulation course 12.Baking is 2 hours in 40-80 ℃ constant temperature oven, takes out to be positioned in the clean container, cools standby; On the electrode 2,3 of above-mentioned preparation, point out bar shaped mix reagent layer 4 with point sample instrument, the composition such as the table 1 of mix reagent, the mix reagent of new configuration need shake up to place and use later in 2 hours, and the point sample amount of each strip is 1 microlitre; Put into the exsiccator that fills silica gel behind the point sample dry 5 hours, and took out on reagent layer 4 lower limb and paste pad 6, add a cover hydrophilic film 7 and form sample cavitys 5.
During use, the contact (10,11) of disposable whole blood uric acid testing electrode indicator bar is connected with miniature Electrochemical Detection instrument, and the uric acid in the blood sucks reagent layer 4 certainly through the injection port of sample cavity 5 one ends, and generate potassium ferrocyanide with potassium ferricyanide generation electrochemical reaction, form and detect magnitude of voltage.
Blood with the uric acid physiological saline titer concentration known of physiological saline and 100mg/dl is adjusted into: 2.5,5,10,15, and 20mg/dl.To detect strip and insert miniature Electrochemical Detection instrument, the above-mentioned blood that takes a morsel places the injection port of the cavity of strip, miniature Electrochemical Detection instrument display voltage value after 10 seconds, and measurement result is shown in Figure 3.100 unknown blood sample are directly measured, and measurement result is compared as shown in Figure 4 with large-scale Biochemical Analyzer result, and its linearly dependent coefficient has reached 99.87%.
Table 1
| Composition | Content |
| Borax solution (0.05mol/L) aqueous dibasic potassium phosphate solution (0.1mol/L) sodium carboxymethyl cellulose (CMC) the bovine serum albumin(BSA) potassium ferricyanide | 6.5mL 3.5mL 2mg 15mg 650mg |
Claims (3)
1. disposable whole blood uric acid testing electrode indicator bar, comprise a dielectric base (1), on dielectric base (1), be provided with working electrode (2) and contrast electrode (3), draw first lead (8) from working electrode (2), make first contact (10) in the termination of first lead (8), draw second lead (9) from contrast electrode (3), make second contact (11) in the termination of second lead (9), cover part working electrode (2), part contrast electrode (3), first lead (8), second lead (9), insulation course (12) on part first contact (10) and part second contact (11), be arranged at the reagent layer (4) on working electrode (2) and the contrast electrode (3), pad (6) is pasted at edge at reagent layer (4), hydrophilic film (7) is added a cover on top, by reagent layer (4), pad (6) and hydrophilic film (7) surround sample cavity (5), it is characterized in that: described reagent layer (4) comprises bovine serum albumin(BSA), electron mediator, cementing agent and pH buffering agent, the pH buffering agent is a borate, any of citrate, its pH value is 8.5, the thickness of pad (6) is the 70-120 micron, and the spacing of two pads is 2 millimeters.
2. according to the disposable whole blood uric acid testing electrode indicator bar of claim 1, it is characterized in that: described electron mediator is the potassium ferricyanide.
3. according to the disposable whole blood uric acid testing electrode indicator bar of claim 2, it is characterized in that: described cementing agent is any of sodium carboxymethyl cellulose, polyvinylpyrrolidone, polyvinyl alcohol (PVA) or polyglycol.
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| CN102735835B (en) * | 2011-04-15 | 2015-03-04 | 佳木斯大学 | Disposable whole-blood uric acid detection test strip and manufacturing method thereof |
| CN103175872A (en) * | 2011-12-23 | 2013-06-26 | 长沙中生众捷生物技术有限公司 | Portable electrochemical detection test strip and preparation method thereof |
| CN104000601A (en) * | 2013-02-22 | 2014-08-27 | 谭唱 | Rapid blood uric acid measuring instrument |
| CN104777194B (en) * | 2015-04-21 | 2017-10-27 | 苏州市玮琪生物科技有限公司 | Anti-interference sensing electrode and micro-fluidic test paper runner flow rate control method |
| CN109540881A (en) * | 2018-12-06 | 2019-03-29 | 桂林优利特医疗电子有限公司 | A kind of analoids |
| CN112858422B (en) * | 2019-11-12 | 2022-12-20 | 北京怡成生物电子技术股份有限公司 | Electrochemical uric acid test strip for removing interference of ascorbic acid and manufacturing and application thereof |
| CN114460147A (en) * | 2022-02-11 | 2022-05-10 | 深圳市溢鑫科技研发有限公司 | Vertical graphene electrochemical microelectrode structure |
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