CN210665581U

CN210665581U - Electrochemical test strip with dual functions of blood sugar and blood ketone

Info

Publication number: CN210665581U
Application number: CN201920808928.0U
Authority: CN
Inventors: 史丙华; 姜志华; 陈玲强; 全昌云; 汪江海
Original assignee: Hunan Haiyuan Medical Technology Co ltd
Current assignee: Hunan Haiyuan Medical Technology Co ltd
Priority date: 2019-05-31
Filing date: 2019-05-31
Publication date: 2020-06-02
Anticipated expiration: 2029-05-31

Abstract

The utility model provides a difunctional electrochemistry examination strip of blood sugar, blood ketone, its includes the PET base plate, and this PET base plate tow sides set up conducting layer, two-sided glue layer, solid-state enzyme layer and hydrophilic rete respectively in order to form the siphon pond that can inhale the blood sample that awaits measuring respectively, and wherein the two electrode access of this conducting layer can carry out erythrocyte backlog and blood sugar/blood ketone test respectively. Therefore, the test strip can respectively suck one sample into the two siphon pools through the two siphon pools, simultaneously detect the blood sugar and the blood ketone in one sample, and the two test values are both subjected to volume calibration, so that the blood sugar and the blood ketone can be conveniently and accurately detected, and the pain caused by multiple blood sampling of a user is reduced; the test strip is matched with a measuring instrument, error prompt caused by different insertion sequences of the front side and the back side of the test strip can be avoided, the instrument can automatically identify test items according to different resistance values of the front side and the back side, and the operation is simple.

Description

Electrochemical test strip with dual functions of blood sugar and blood ketone

Technical Field

The utility model belongs to external diagnosis product field, concretely relates to difunctional electrochemistry examination strip that ability simultaneous determination blood sugar and blood ketone and can carry out the hematocrit correction.

Background

With the development of the in vitro diagnosis industry, various medical products are gradually emitted, the demand of consumers on simple and multifunctional medical diagnosis products is continuously increased, high-quality products are continuously pursued, the accuracy of results is ensured, and meanwhile, medical products capable of measuring in one form and multiple times are continuously sought, so that the in vitro diagnosis products are promoted to develop towards multiple functions.

With the continuous change of modern life, the number of chronic patients is gradually increased, especially for the diabetic population, when diabetes is aggravated, insulin is absolutely absent, three major metabolic disorders are disturbed, not only blood sugar is obviously increased, but also lipolysis is increased, fatty acid is oxidized by β in the liver to generate a large amount of acetyl coenzyme A, and the acetyl coenzyme A cannot enter the tricarboxylic acid cycle oxidation function and is condensed into a whole body due to sugar metabolic disorder and insufficient oxaloacetate, and simultaneously, due to the reduction of protein synthesis and the increase of decomposition, the increase of blood sugar and ketone-forming amino acid leads to further increase of blood sugar and blood ketone, and serious ketosis is caused.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a difunctional electrochemistry examination strip of blood sugar, blood ketone to the not enough of above-mentioned prior art, it sets up the reaction zone respectively in the positive and negative of PET base plate to test blood sugar and blood ketone respectively.

In order to achieve the above purpose, the technical scheme adopted by the utility model is as follows: a difunctional electrochemical test strip of blood sugar, blood ketone, it includes PET plaque, conducting layer with passage of electrode, double-sided glue layer, solid enzyme layer and hydrophilic membranous layer to have punching a hole, characterized by that, said conducting layer, double-sided glue layer, solid enzyme layer and hydrophilic membranous layer are two layers, two conducting layers locate the positive and negative of the PET plaque separately, the double-sided glue layer locates on the conducting layer and reveals some wires of the passage of electrode; the solid enzyme layer is arranged in the punched hole of the double-sided adhesive layer and is connected with the lead of the electrode passage of the exposed conducting layer, one enzyme layer in the two solid enzyme layers can react with the blood sugar in the blood sample to be detected, and the other enzyme layer can react with the blood ketone in the blood sample to be detected; the hydrophilic film layer is covered on the double-sided adhesive layer; thus, two siphon pools capable of sucking blood samples to be measured are formed by the two conducting layers, the two double-sided glue layers, the two solid enzyme layers and the two hydrophilic film layers.

The electrode paths of the conducting layer are divided into two groups, wherein one group of electrode paths consists of a first working electrode and a first counter electrode and is used for testing hematocrit, and the other group of electrode paths consists of a second working electrode and a second counter electrode and is used for testing blood sugar or blood ketone.

One of the two conductive layers has a resistance value of 40-65 ohms, and the other conductive layer has a resistance value of 120-150 ohms.

Therefore, the test strip can suck one sample into two siphon pools respectively by arranging the siphon pools on the front side and the back side of the PET substrate respectively, simultaneously detect the blood sugar and the blood ketone in one sample, and calibrate the two test values by pressure accumulation, so that the blood sugar and the blood ketone can be detected conveniently and accurately, and the pain caused by blood sampling for a plurality of times of a user is reduced; the test strip is matched with a measuring instrument, error prompt caused by different insertion sequences of the front side and the back side of the test strip can be avoided, the instrument can automatically identify test items according to different resistance values of the front side and the back side, and the operation is simple.

Drawings

Fig. 1 is an exploded view of the present invention.

Fig. 2 is a structure diagram of the conductive layer electrode of the present invention.

Fig. 3 is a schematic view of the front or back of the present invention.

FIG. 4 is a cross-sectional view of a test strip of the present invention.

Detailed Description

Referring to fig. 1 and 4, the utility model relates to a bifunctional electrochemical test strip for blood sugar and blood ketone, which comprises a PET substrate 1, wherein conductive layers 2 are respectively arranged on the front and back sides of the PET substrate 1; the conductive layer 2 is formed by printing a gold electrode on the PET substrate 1, and the resistance values of the two conductive layers 2 are 40-65 ohm or 120-150 ohm respectively (i.e. when the resistance value of one conductive layer is 120-150 ohm, the resistance value of the other conductive layer is 40-65 ohm); referring to fig. 2, two sets of electrode paths are disposed on the conductive layer 2, wherein one set of electrode paths is composed of a first working electrode 21 and a first counter electrode 22 for testing hematocrit, and the other set of electrode paths is composed of a second working electrode 23 and a second counter electrode 24 for testing blood glucose or blood ketone; a double-sided adhesive layer 3 is arranged on the conducting layer 2; one end of the double-sided adhesive layer 3 is provided with a punching hole 31, so that when the double-sided adhesive layer 3 covers the conductive layer 2, part of the leads of the two sets of electrode paths are exposed (as shown in fig. 3); the punching hole 31 of the double-sided adhesive layer 3 is internally provided with a solid enzyme layer 4, the solid enzyme layer 4 is connected with leads of two groups of electrode paths of the conducting layer 2, one enzyme layer of the two solid enzyme layers 4 can react with blood sugar in a blood sample, and the other enzyme layer can react with blood ketone in the blood sample; the double-sided adhesive layer 3 is covered with a hydrophilic film layer 5. Thus, two siphon pools capable of sucking blood samples to be tested are formed by the two conducting layers 2, the two double-sided adhesive layers 3, the two solid enzyme layers 4 and the two hydrophilic film layers 5.

The preparation of the two solid enzyme layers mentioned above is a conventional technique in the art, for example, one of the two solid enzyme layers contains glucose oxidase or glucose dehydrogenase for measuring blood sugar, the other solid enzyme layer contains β -hydroxybutyrate dehydrogenase and coenzyme NAD + for measuring blood ketone, the enzyme layer may further contain an electron mediator, a surfactant and a buffer solution, wherein the electron mediator is one of potassium ferricyanide, ruthenium complex, ferrocene and derivatives thereof, the surfactant is polyoxyethylene ether, cellulose or alkylpyridine, the buffer solution is phosphate buffer solution, Tris-HCl, citrate buffer solution or acetate buffer solution, and the pH value is 5.3-7.5.

When the test strip is used, because the test strip is very thin, when a blood sample to be tested is close to the punched hole 31, the blood sample to be tested is respectively sucked into siphon pools where solid enzyme layers on the front side and the back side of a PET substrate are located due to the siphon action, the hematocrit test is firstly carried out by the first working electrode 21 and the first counter electrode 22 of the two conducting layers in cooperation with a blood glucose and blood ketone tester, the blood glucose value and the blood ketone value are corrected, and then the blood glucose value or the blood ketone value is tested through the second working electrode 23 and the second counter electrode 24 of the two conducting layers, so that the blood glucose value and the blood ketone value can be simultaneously tested by one blood sample.

The above embodiments are only for further illustration of the present solution, and are not intended to limit the protection scope of the present invention.

Claims

1. A difunctional electrochemical test strip of blood sugar, blood ketone, it includes PET plaque (1), conducting layer (2) with electrode passage, one pair of glue layers (3) with punch hole (31), solid enzyme layer (4) and hydrophilic membranous layer (5), characterized by that, said conducting layer (2), one pair of glue layers (3), one pair of enzyme layers (4) and hydrophilic membranous layer (5) are two layers, two conducting layers locate the positive and negative of the PET plaque (1) separately, the one pair of glue layers (3) locates on the conducting layer (2) and reveals some wires of the electrode passage; the solid enzyme layer (4) is arranged in the punched hole (31) of the double-sided adhesive layer (3) and is connected with a lead of an electrode passage of the exposed conducting layer (2), one enzyme layer of the two solid enzyme layers (4) can react with blood sugar in a blood sample to be detected, and the other enzyme layer can react with blood ketone in the blood sample to be detected; the hydrophilic film layer (5) is covered on the double-sided adhesive layer (3); thus, two siphon pools which can suck blood samples to be tested are formed by the two conducting layers (2), the two double-sided glue layers (3), the two solid enzyme layers (4) and the two hydrophilic film layers (5);

the electrode paths of the conducting layer (2) are divided into two groups, wherein one group of electrode paths consists of a first working electrode (21) and a first counter electrode (22) and is used for testing the hematocrit, and the other group of electrode paths consists of a second working electrode (23) and a second counter electrode (24) and is used for testing the blood sugar or the blood ketone.

2. The dual-functional electrochemical test strip for blood glucose and blood ketone as claimed in claim 1, wherein one of the two conductive layers (2) has a resistance value of 40-65 ohms, and the other conductive layer has a resistance value of 120-150 ohms.