CN201886023U - Test bar for binary detection of beta-stimulant ractopamine and salbutamol - Google Patents
Test bar for binary detection of beta-stimulant ractopamine and salbutamol Download PDFInfo
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- CN201886023U CN201886023U CN2010202699338U CN201020269933U CN201886023U CN 201886023 U CN201886023 U CN 201886023U CN 2010202699338 U CN2010202699338 U CN 2010202699338U CN 201020269933 U CN201020269933 U CN 201020269933U CN 201886023 U CN201886023 U CN 201886023U
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- ractopamine
- salbutamol
- beta
- glass fibre
- glass fiber
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Abstract
The utility model relates to a test bar for binary detection of beta-stimulant ractopamine and salbutamol, which can effectively solve the problem that the beta-stimulant ractopamine and salbutamol can not be quickly and simply detected simultaneously. The technical scheme for solving the problem is as follows: a base material PVC (Poly Vinyl Chloride) plate is sequentially provided with water-guiding glass fiber, vector glass fiber, a nitrocellulose membrane and a water-absorbing pulp board from front to back, wherein the water-guiding glass fiber, the vector glass fiber and the water-absorbing pulp board are covered with a tectorial membrane; the vector glass fiber is internally absorbed with a colloidal gold marker of anti-ractopamine protein coupling contents, monoclonal antibodies, and anti-salbutamol protein coupling contents, monoclonal antibodies; the nitrocellulose membrane is wrapped with a quality-control line and two testing lines; the quality-control line is wrapped with rabbit anti-mouse polyclonal antibodies; and the testing line is wrapped with the ractopamine protein coupling contents and the salbutamol protein coupling contents. The test bar can be effectively used for detecting the beta-stimulant ractopamine and the salbutamol simultaneously, is simple, convenient and fast, and is accurate in testing.
Description
One, technical field
The utility model relates to medical equipment, and particularly a kind of beta-stimulants Ractopamine, salbutamol binary detect test-strips.
Two, background technology
Beta-stimulants is a class adrenomimetic drug, because of its can make the beta receptor excitement (excitement) in the animal and human soma thus the function of different tissues is gained the name in the control agent, its chemical constitution is a Phenethanolamine derivative, such medicine has the livestock and poultry of promotion lipolysis, increases the muscle growth effect, as the heavy partitioning agent of nutrition, to improve lean meat percentage, reduce fat deposition and promote growth of animal, illegally used as culturing promoter by some livestock-raising persons.The most frequently used in aquaculture is Clenizole Hydrochloride (Clenbuterol), salbutamol (Salbutamol) and Ractopamine (ractopamine), uses beta-stimulants and cause consumer's food poisoning to happen occasionally in feed stripped.Beta-stimulants is as the feed addictive that promotes growth of animal, raising lean meat percentage; but long-term use can make such medicine accumulate in animal foodstuff; the people eaten feed contain the livestock products of beta-stimulants as the adjuvant feed after; toxicity symptom will appear; muscular tremor, palpitaition, stress, headache, myalgia, dizzy, symptom such as feel sick, vomit, have a fever, tremble can appear usually, in addition dead.Therefore, world many countries forbids in feed using beta-stimulants such as clenobuterol hydrochloride to increase the lean meat percentage of animal.The Chinese government has prohibited beta-stimulants such as using clenobuterol hydrochloride in herding is produced.
At present, prior art mainly relies on chemical apparatuses analysis, enzyme immunoassay kit and the independent detection test-strips that detects Clenizole Hydrochloride, salbutamol and Ractopamine for the detection of Clenizole Hydrochloride, salbutamol and Ractopamine.Supervision department does not also know which kind of beta-agonist the raiser has used, and detects to have certain blindness, usually causes omission.Therefore, market presses for a kind of reagent can detect common beta-agonist simultaneously, to make things convenient for routine monitoring work.
Three, utility model content
At above-mentioned situation, for overcoming the prior art defective, the utility model purpose is to provide a kind of energy quick, detect the beta-stimulants Ractopamine easily simultaneously, the salbutamol binary detects test-strips, for achieving the above object, the utility model adopts following technical scheme: comprise base material PVC plate and overlay film, be disposed with the diversion glass fibre from front to back above the base material PVC plate, the carrier glass fibre, nitrocellulose membrane and absorbent wool pulpboard, the diversion glass fibre, carrier glass fibre and be coated with overlay film (the alleged anterior coverlay of forming by anterior coverlay and rear portion coverlay above the absorbent wool pulpboard, the rear portion coverlay is that specimen samples swimming direction is as the criterion when using with test-strips, before the diversion end is, the suction side is the back), be adsorbed with anti-Ractopamine protein conjugate monoclonal antibody and desertification butylamine alcohol protein conjugate monoclonal antibody colloid gold label thing in the carrier glass fibre, be coated with a nature controlling line and two p-wires on the nitrocellulose membrane, nature controlling line is coated with the anti-mouse of rabbit and resists (known commercially available prod more, product as Zhengzhou Lan Sen Bioisystech Co., Ltd), p-wire is coated with the Ractopamine protein conjugate, the salbutamol protein conjugate; The nitrocellulose membrane two ends closely link to each other with absorbent wool pulpboard front end face with carrier glass fibre rear end face respectively, carrier glass fibre front end face closely links to each other with diversion glass fibre rear end face, the utility model can be effective to detect simultaneously beta-stimulants Ractopamine, salbutamol, and simple, convenient, fast, test accurately.
Four, description of drawings
Accompanying drawing is the utility model test-strips three-dimensional structure diagram (overlay film starts).
Five, embodiment
Below in conjunction with accompanying drawing embodiment of the present utility model is elaborated.
Provide by accompanying drawing, the utility model comprises base material PVC plate and overlay film, be disposed with diversion glass fibre 3 from front to back above the base material PVC plate 1, carrier glass fibre 4, nitrocellulose membrane 2 and absorbent wool pulpboard 5, the diversion glass fibre, carrier glass fibre and be coated with overlay film (the alleged anterior coverlay of forming by anterior coverlay 7 and rear portion coverlay 67 above the absorbent wool pulpboard, rear portion coverlay 6 is that specimen samples swimming direction is as the criterion when using with test-strips, before the diversion end is, the suction side is the back), be adsorbed with anti-Ractopamine protein conjugate monoclonal antibody and desertification butylamine alcohol protein conjugate monoclonal antibody colloid gold label thing in the carrier glass fibre, be coated with a nature controlling line and two p-wires on the nitrocellulose membrane, it is how anti-that nature controlling line is coated with the anti-mouse of rabbit, and p-wire is coated with the Ractopamine protein conjugate, the salbutamol protein conjugate; The nitrocellulose membrane two ends closely link to each other with absorbent wool pulpboard front end face with carrier glass fibre rear end face respectively, and carrier glass fibre front end face closely links to each other with diversion glass fibre rear end face.
In order to guarantee result of use, said overlay film is a pressure sensitive membrane.
It is to be noted; the utility model creative contribution is; with prior art through scientific and reasonable combination and configuration; constitute novel beta-stimulants Ractopamine, salbutamol binary detection test-strips; be effective to detect simultaneously beta-stimulants Ractopamine, salbutamol, the technical scheme that all that adopt structurally that equivalent replacement means have done and the utility model do not have essential distinction in essence all belongs to protection domain of the present utility model.
The utility model can be effective to detect simultaneously beta-stimulants Ractopamine, salbutamol, the utility model test-strips in use, vertically insert diversion glass fibre (film) end in the specimen samples, or specimen samples dripped to the anterior diversion glass fibre of test-strips, sample is under capillary action, along test-strips to rear portion absorbent wool pulpboard direction swimming, if contain beta-stimulants Ractopamine and salbutamol or a kind of in the specimen samples, they and respective markers antibody (colloid gold label monoclonal antibody) reaction form labelled antibody-antigenic compound; When the specimen samples swimming does not then react when Ractopamine protein conjugate, salbutamol protein conjugate detect band to being coated with, and with the nature controlling line reaction, a red line (nature controlling line) or two red lines only appear in the result; If no beta-stimulants Ractopamine and salbutamol in the specimen samples, labelled antibody (desertification butylamine alcohol protein conjugate monoclonal antibody or anti-Ractopamine protein conjugate monoclonal antibody) combines with Ractopamine protein conjugate, salbutamol protein conjugate and nature controlling line on the coated film respectively and forms the red line band, and n+1 bar red line appears in the result; Red line do not occur as nature controlling line, show that then test-strips lost efficacy.
The utility model is through test, utilize this test-strips to test beta-stimulants Ractopamine, salbutamol simultaneously, only needed to test beta-stimulants Ractopamine, salbutamol in one time application of sample 5-10 minute, on probation through 158 times, except that 1 example is indeterminate, all the other 157 examples have all reached promising result, rate of accuracy reached is more than 99%, therefore, the method that the utlity model has is simple, the characteristics convenient and swift, that the result is accurate are the innovations on beta-stimulants Ractopamine, the salbutamol checkout equipment.
Claims (2)
1. a beta-stimulants Ractopamine, salbutamol binary detect test-strips, comprise base material PVC plate and overlay film, it is characterized in that, be disposed with diversion glass fibre (3), carrier glass fibre (4), nitrocellulose membrane (2) and absorbent wool pulpboard (5) above the base material PVC plate (1) from front to back, diversion glass fibre, carrier glass fibre and be coated with the overlay film of forming by anterior coverlay (7) and rear portion coverlay (6) above the absorbent wool pulpboard, be coated with a nature controlling line and two p-wires on the nitrocellulose membrane, it is how anti-that nature controlling line is coated with the anti-mouse of rabbit; The nitrocellulose membrane two ends closely link to each other with absorbent wool pulpboard front end face with carrier glass fibre rear end face respectively, and carrier glass fibre front end face closely links to each other with diversion glass fibre rear end face.
2. beta-stimulants Ractopamine according to claim 1, salbutamol binary detect test-strips, it is characterized in that said overlay film is a pressure sensitive membrane.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010202699338U CN201886023U (en) | 2010-07-26 | 2010-07-26 | Test bar for binary detection of beta-stimulant ractopamine and salbutamol |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010202699338U CN201886023U (en) | 2010-07-26 | 2010-07-26 | Test bar for binary detection of beta-stimulant ractopamine and salbutamol |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201886023U true CN201886023U (en) | 2011-06-29 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010202699338U Expired - Fee Related CN201886023U (en) | 2010-07-26 | 2010-07-26 | Test bar for binary detection of beta-stimulant ractopamine and salbutamol |
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| CN (1) | CN201886023U (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101915842A (en) * | 2010-07-26 | 2010-12-15 | 河南省科学院生物研究所有限责任公司 | Binary detection test strip of beta-exhilarant ractopamine and salbutamol and preparation method thereof |
| CN102608317A (en) * | 2012-02-23 | 2012-07-25 | 北京维德维康生物技术有限公司 | Kit or test strips for detecting salbutamol |
| CN102901821A (en) * | 2012-09-27 | 2013-01-30 | 江苏维赛科技生物发展有限公司 | Colloidal gold detection card for detecting adrenoreceptor stimulant and sample processing method |
| CN107942052A (en) * | 2017-09-29 | 2018-04-20 | 杭州南开日新生物技术有限公司 | A kind of colloidal gold kit for detecting beta-agonist class medicine and preparation method thereof |
-
2010
- 2010-07-26 CN CN2010202699338U patent/CN201886023U/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101915842A (en) * | 2010-07-26 | 2010-12-15 | 河南省科学院生物研究所有限责任公司 | Binary detection test strip of beta-exhilarant ractopamine and salbutamol and preparation method thereof |
| CN101915842B (en) * | 2010-07-26 | 2013-04-03 | 河南省科学院生物研究所有限责任公司 | Binary detection test strip of beta-exhilarant ractopamine and salbutamol and preparation method thereof |
| CN102608317A (en) * | 2012-02-23 | 2012-07-25 | 北京维德维康生物技术有限公司 | Kit or test strips for detecting salbutamol |
| CN102608317B (en) * | 2012-02-23 | 2014-01-01 | 北京维德维康生物技术有限公司 | Kit or test strips for detecting salbutamol |
| CN102901821A (en) * | 2012-09-27 | 2013-01-30 | 江苏维赛科技生物发展有限公司 | Colloidal gold detection card for detecting adrenoreceptor stimulant and sample processing method |
| CN107942052A (en) * | 2017-09-29 | 2018-04-20 | 杭州南开日新生物技术有限公司 | A kind of colloidal gold kit for detecting beta-agonist class medicine and preparation method thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20110629 Termination date: 20130726 |