CN201828563U - Test strip for clenbuterol and ractopamine binary-detection of beta-stimulant - Google Patents
Test strip for clenbuterol and ractopamine binary-detection of beta-stimulant Download PDFInfo
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- CN201828563U CN201828563U CN2010202699107U CN201020269910U CN201828563U CN 201828563 U CN201828563 U CN 201828563U CN 2010202699107 U CN2010202699107 U CN 2010202699107U CN 201020269910 U CN201020269910 U CN 201020269910U CN 201828563 U CN201828563 U CN 201828563U
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- ractopamine
- glass fibre
- beta
- clenbuterol
- protein conjugate
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Abstract
The utility model relates to a test strip for the clenbuterol and ractopamine binary-detection of a beta-stimulant, which solves the problem of effectively, quickly, simply, conveniently and simultaneously detecting clenbuterol and ractopamine of the beta-stimulant. The technical scheme is as follows water diversion glass fibers, carrier glass fibers, a nitrocellulose membrane and an absorbent cotton pulp board are sequentially arranged on a base material, the water diversion glass fibers, the carrier glass fibers, the nitrocellulose membrane and the absorbent cotton pulp board are provided with a covering membrane which comprises affront covering membrane and a back covering membrane, the carrier glass fibers of the front part of the test part are absorbed with anti-clenbuterol protein conjugate and anti-ractopamine protein conjugate monoclonal antibody colloidal gold marker, and one quality control line covered with a rabbit-anti-mouse polyclonal antibody and two detection lines which are respectively covered with clenbuterol protein conjugate and ractopamine protein conjugate are distributed on the nitrocellulose membrane at the middle part from back to front. The utility model can effectively simultaneously detect the clenbuterol and ractopamine dual-detection, is convenient and quick, and has accurate result.
Description
One, technical field
The utility model relates to beta-stimulants detection technique field, and particularly a kind of beta-stimulants clenobuterol hydrochloride, Ractopamine binary detect test-strips.
Two, background technology
Beta-stimulants is a class adrenomimetic drug, because of its can make the beta receptor excitement (excitement) in the animal and human soma thus the function of different tissues is gained the name in the control agent, its chemical constitution is a Phenethanolamine derivative, such medicine has the livestock and poultry of promotion lipolysis, increases the muscle growth effect, as the heavy partitioning agent of nutrition, to improve lean meat percentage, reduce fat deposition and promote growth of animal, illegally used as culturing promoter by some livestock-raising persons.The most frequently used in aquaculture is Clenizole Hydrochloride (Clenbuterol), salbutamol (Salbutamol) and Ractopamine (ractopamine), uses beta-stimulants and cause consumer's food poisoning to happen occasionally in feed stripped.Beta-stimulants is as the feed addictive that promotes growth of animal, raising lean meat percentage; but long-term use can make such medicine accumulate in animal foodstuff; the people eaten feed contain the livestock products of beta-stimulants as the adjuvant feed after; toxicity symptom will appear; muscular tremor, palpitaition, stress, headache, myalgia, dizzy, symptom such as feel sick, vomit, have a fever, tremble can appear usually, in addition dead.Therefore, world many countries forbids in feed using beta-stimulants such as clenobuterol hydrochloride to increase the lean meat percentage of animal.The Chinese government has prohibited beta-stimulants such as using clenobuterol hydrochloride in herding is produced.
At present, prior art mainly relies on chemical apparatuses analysis, enzyme immunoassay kit and the independent detection test-strips that detects Clenizole Hydrochloride, salbutamol and Ractopamine for the detection of Clenizole Hydrochloride, salbutamol and Ractopamine.Supervision department does not also know which kind of beta-agonist the raiser has used, and detects to have certain blindness, usually causes omission.Therefore, market presses for a kind of reagent can detect common beta-agonist simultaneously, to make things convenient for routine monitoring work.
Three, utility model content
At above-mentioned situation, for overcoming the prior art defective, the purpose of the utility model just provides a kind of beta-stimulants clenobuterol hydrochloride, the Ractopamine binary detects test-strips, can effectively solve fast, detect the beta-stimulants clenobuterol hydrochloride easily simultaneously, the problem of Ractopamine, the technical scheme of its solution is, this test-strips comprises base material PVC plate and overlay film, be disposed with the diversion glass fibre from front to back above the base material PVC plate, the carrier glass fibre, nitrocellulose membrane and absorbent wool pulpboard, the diversion glass fibre, carrier glass fibre and be coated with the overlay film of being made up of anterior coverlay and rear portion coverlay above the absorbent wool pulpboard is adsorbed with anti-clenobuterol hydrochloride protein conjugate on the anterior carrier glass fibre of test-strips, anti-Ractopamine protein conjugate monoclonal antibody colloid gold label thing; On the nitrocellulose membrane of middle part from the back and before be distributed with one and be coated with that the anti-mouse of rabbit is how anti-(to be known commercially available prod, product as Zhengzhou Lan Sen Bioisystech Co., Ltd) nature controlling line, article two, be coated with the clenobuterol hydrochloride protein conjugate respectively, the detection line of Ractopamine protein conjugate is (before said, after be meant with the diversion direction to the suction direction, before the diversion glass fibre is, the absorbent wool pulpboard is the back), the nitrocellulose membrane two ends closely link to each other with absorbent wool pulpboard front end face with carrier glass fibre rear end face respectively, and carrier glass fibre front end face closely links to each other with diversion glass fibre rear end face;
The utility model can be effective to detect clenobuterol hydrochloride, Ractopamine simultaneously, and method is simple, and is convenient, fast, and the result is accurate.
Four, description of drawings
Accompanying drawing is the utility model test-strips three-dimensional structure diagram (overlay film starts).
Five, embodiment
Below in conjunction with accompanying drawing embodiment of the present utility model is elaborated.
Provide by accompanying drawing, the utility model binary detects its structure of test-strips, comprise base material PVC plate and overlay film, be disposed with diversion glass fibre 3, carrier glass fibre 4, nitrocellulose membrane 2 and absorbent wool pulpboard 5 above the base material PVC plate 1 from front to back, diversion glass fibre 3, carrier glass fibre 4 and be coated with the overlay film of being made up of anterior coverlay 7 and rear portion coverlay 6 above the absorbent wool pulpboard 5 are adsorbed with anti-clenobuterol hydrochloride protein conjugate, anti-Ractopamine protein conjugate monoclonal antibody colloid gold label thing on the anterior carrier glass fibre of test-strips; Precedingly be distributed with one and be coated with the how anti-nature controlling lines of the anti-mouse of rabbit from the back on the nitrocellulose membrane of middle part, article two, be coated with detection line (said forward and backward being meant with the diversion direction of clenobuterol hydrochloride protein conjugate, Ractopamine protein conjugate respectively to the suction direction, before the diversion glass fibre is, the absorbent wool pulpboard is the back), the nitrocellulose membrane two ends closely link to each other with absorbent wool pulpboard front end face with carrier glass fibre rear end face respectively, and carrier glass fibre front end face closely links to each other with diversion glass fibre rear end face.
In order to guarantee result of use, said overlay film is a pressure sensitive membrane.
It is to be noted; the utility model creative contribution is; with prior art through scientific and reasonable combination and configuration; constitute novel beta-stimulants clenobuterol hydrochloride, Ractopamine binary detection test-strips; be effective to detect simultaneously beta-stimulants clenobuterol hydrochloride, Ractopamine, the technical scheme that all that adopt structurally that equivalent replacement means have done and the utility model do not have essential distinction in essence all belongs to protection domain of the present utility model.
The utility model test-strips is vertically inserted diversion glass fibre membrane one end in the specimen samples in use, or specimen samples is dripped to test-strips bottom diversion glass fibre.Sample is under capillary action, along test-strips to the swimming of absorbent wool pulpboard direction, if contain beta-stimulants clenobuterol hydrochloride and Ractopamine or a kind of in the specimen samples, they and respective markers antibody (colloid gold label monoclonal antibody) reaction form labelled antibody-antigenic compound.When the specimen samples swimming does not then react when clenobuterol hydrochloride protein conjugate, Ractopamine protein conjugate detect band to being coated with, and with the nature controlling line reaction, a red line (nature controlling line) or two red lines only appear in the result; If no beta-stimulants clenobuterol hydrochloride and Ractopamine in the specimen samples, labelled antibody (anti-clenobuterol hydrochloride protein conjugate monoclonal antibody or anti-Ractopamine protein conjugate monoclonal antibody) combines with clenobuterol hydrochloride protein conjugate, Ractopamine protein conjugate and nature controlling line on the coated film respectively and forms the red line band, and n+1 bar red line appears in the result; Red line do not occur as nature controlling line, show that then test-strips lost efficacy.Therefore, utilize this test-strips, only need an application of sample, can detect two kinds of beta-stimulants in 5-10 minute.This method is simple, convenient, fast, the result is accurate.
The utility model is through test, utilize this test-strips to test beta-stimulants clenobuterol hydrochloride, Ractopamine simultaneously, only need an application of sample, can test beta-stimulants clenobuterol hydrochloride, Ractopamine in 5-10 minute, on probation through 158 times, except that 1 example is indeterminate, all the other 157 examples have all reached promising result, rate of accuracy reached is more than 99%, therefore, the method that the utlity model has is simple, and is convenient and swift, the characteristics that the result is accurate are the innovations on beta-stimulants clenobuterol hydrochloride, the Ractopamine checkout equipment.
Claims (2)
1. beta-stimulants clenobuterol hydrochloride, the Ractopamine binary detects test-strips, comprise base material PVC plate and overlay film, it is characterized in that, be disposed with diversion glass fibre (3) above the base material PVC plate (1) from front to back, carrier glass fibre (4), nitrocellulose membrane (2) and absorbent wool pulpboard (5), diversion glass fibre (3), carrier glass fibre (4) and be coated with the overlay film of forming by anterior coverlay (7) and rear portion coverlay (6) above the absorbent wool pulpboard (5), precedingly be distributed with one and be coated with the how anti-nature controlling lines of the anti-mouse of rabbit from the back on the nitrocellulose membrane of middle part, article two, be coated with the clenobuterol hydrochloride protein conjugate respectively, the detection line of Ractopamine protein conjugate, the nitrocellulose membrane two ends closely link to each other with absorbent wool pulpboard front end face with carrier glass fibre rear end face respectively, and carrier glass fibre front end face closely links to each other with diversion glass fibre rear end face.
2. beta-stimulants clenobuterol hydrochloride according to claim 1, Ractopamine binary detect test-strips, it is characterized in that said overlay film is a pressure sensitive membrane.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010202699107U CN201828563U (en) | 2010-07-26 | 2010-07-26 | Test strip for clenbuterol and ractopamine binary-detection of beta-stimulant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010202699107U CN201828563U (en) | 2010-07-26 | 2010-07-26 | Test strip for clenbuterol and ractopamine binary-detection of beta-stimulant |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201828563U true CN201828563U (en) | 2011-05-11 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010202699107U Expired - Fee Related CN201828563U (en) | 2010-07-26 | 2010-07-26 | Test strip for clenbuterol and ractopamine binary-detection of beta-stimulant |
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| Country | Link |
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| CN (1) | CN201828563U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106370843A (en) * | 2016-08-25 | 2017-02-01 | 陈军 | Quick measurement method of lean meat powder on the basis of gold magnetic immuno-chromatography |
-
2010
- 2010-07-26 CN CN2010202699107U patent/CN201828563U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106370843A (en) * | 2016-08-25 | 2017-02-01 | 陈军 | Quick measurement method of lean meat powder on the basis of gold magnetic immuno-chromatography |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20110511 Termination date: 20130726 |