CN201828562U - Test strip for salbutamol, clenbuterol and ractopamine ternary-detection of Beta-stimulant - Google Patents
Test strip for salbutamol, clenbuterol and ractopamine ternary-detection of Beta-stimulant Download PDFInfo
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- CN201828562U CN201828562U CN201020269898XU CN201020269898U CN201828562U CN 201828562 U CN201828562 U CN 201828562U CN 201020269898X U CN201020269898X U CN 201020269898XU CN 201020269898 U CN201020269898 U CN 201020269898U CN 201828562 U CN201828562 U CN 201828562U
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- ractopamine
- protein conjugate
- beta
- salbutamol
- glass fibre
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Abstract
The utility model relates to a test strip for salbutamol, clenbuterol and ractopamine ternary-detection of a beta-stimulant, which solves the problem of quickly, simply, conveniently and simultaneously detecting salbutamol, clenbuterol and ractopamine of the beta-stimulant. The technical scheme is as follows water diversion glass fibers, carrier glass fibers, a nitrocellulose membrane and an absorbent cotton pulp board are sequentially arranged on a base material, the water diversion glass fibers, the carrier glass fibers, the nitrocellulose membrane and the absorbent cotton pulp board are provided with a covering membrane, the carrier glass fibers are provided with anti-salbutamol protein conjugate and anti-clenbuterol hydrochloride protein conjugate, anti-ractopamine protein conjugate monoclonal antibody colloidal gold marker, and one quality control line covered with a rabbit-anti-mouse polyclonal antibody and the three detection lines which are respectively covered with salbutamol protein conjugate, clenbuterol protein conjugate and ractopamine protein conjugate are distributed on the nitrocellulose membrane at the middle part. The test strip can effectively detect the salbutamol, clenbuterol and ractopamine of the beta-stimulant, is convenient and quick, and has accurate result.
Description
One, technical field
The utility model relates to beta-stimulants detection technique field, particularly relates to a kind of beta-stimulants albuterol, clenobuterol hydrochloride, Ractopamine ternary detection test bar.
Two, background technology
Beta-stimulants is a class adrenomimetic drug, because of its can make the beta receptor excitement (excitement) in the animal and human soma thus the function of different tissues is gained the name in the control agent, its chemical constitution is a Phenethanolamine derivative, such medicine has the livestock and poultry of promotion lipolysis, increases the muscle growth effect, as the heavy partitioning agent of nutrition, to improve lean meat percentage, reduce fat deposition and promote growth of animal, illegally used as culturing promoter by some livestock-raising persons.The most frequently used in aquaculture is Clenizole Hydrochloride (Clenbuterol), salbutamol (Salbutamol) and Ractopamine (ractopamine), uses beta-stimulants and cause consumer's food poisoning to happen occasionally in feed stripped.Beta-stimulants is as the feed addictive that promotes growth of animal, raising lean meat percentage; but long-term use can make such medicine accumulate in animal foodstuff; the people eaten feed contain the livestock products of beta-stimulants as the adjuvant feed after; toxicity symptom will appear; muscular tremor, palpitaition, stress, headache, myalgia, dizzy, symptom such as feel sick, vomit, have a fever, tremble can appear usually, in addition dead.Therefore, world many countries forbids in feed using beta-stimulants such as clenobuterol hydrochloride to increase the lean meat percentage of animal.The Chinese government has prohibited beta-stimulants such as using clenobuterol hydrochloride in herding is produced.
At present, prior art mainly relies on chemical apparatuses analysis, enzyme immunoassay kit and the independent detection test-strips that detects Clenizole Hydrochloride, salbutamol and Ractopamine for the detection of Clenizole Hydrochloride, salbutamol and Ractopamine.Supervision department does not also know which kind of beta-agonist the raiser has used, and detects to have certain blindness, usually causes omission.Therefore, market presses for a kind of reagent can detect common beta-agonist simultaneously, to make things convenient for routine monitoring work.
Three, utility model content
At above-mentioned situation, for overcoming the prior art defective, the purpose of the utility model just provides a kind of beta-stimulants albuterol, clenobuterol hydrochloride, the Ractopamine ternary detection test bar, can effectively solve can be fast, detect beta-stimulants albuterol easily simultaneously, clenobuterol hydrochloride, the problem of Ractopamine, the technical scheme of its solution is, this test-strips comprises base material PVC plate and overlay film, be disposed with the diversion glass fibre from front to back above the base material PVC plate, the carrier glass fibre, nitrocellulose membrane and absorbent wool pulpboard, the diversion glass fibre, carrier glass fibre and be coated with the overlay film of being made up of anterior coverlay and rear portion coverlay above the absorbent wool pulpboard is adsorbed with desertification butylamine alcohol protein conjugate on the carrier glass fibre, anti-clenobuterol hydrochloride protein conjugate, anti-Ractopamine protein conjugate monoclonal antibody colloid gold label thing; The middle part is distributed with one on the nitrocellulose membrane from back to front and is coated with that the anti-mouse of rabbit is how anti-(to be known commercially available prod, product as Zhengzhou Lan Sen Bioisystech Co., Ltd) nature controlling line is (before said, after be meant with the diversion direction to the suction direction, before the diversion glass fibre is, the absorbent wool pulpboard is the back), article three, be coated with the salbutamol protein conjugate respectively, the clenobuterol hydrochloride protein conjugate, the detection line of Ractopamine protein conjugate, the nitrocellulose membrane two ends closely link to each other with absorbent wool pulpboard front end face with carrier glass fibre rear end face respectively, and carrier glass fibre front end face closely links to each other with diversion glass fibre rear end face;
The utility model can be effective to detect beta-stimulants albuterol, clenobuterol hydrochloride, Ractopamine simultaneously, and method is simple, and is convenient, fast, and the result is accurate.
Four, description of drawings
Accompanying drawing is the utility model test-strips three-dimensional structure diagram (overlay film starts).
Five, embodiment
Below in conjunction with accompanying drawing embodiment of the present utility model is elaborated.
Shown in accompanying drawing, the utility model ternary detection test bar comprises base material PVC plate and overlay film, be disposed with diversion glass fibre 3 from front to back above the base material PVC plate 1, carrier glass fibre 4, nitrocellulose membrane 2 and absorbent wool pulpboard 5, diversion glass fibre 3, carrier glass fibre 4 and be coated with the overlay film of being made up of anterior coverlay 7 and rear portion coverlay 6 above the absorbent wool pulpboard 5 is adsorbed with desertification butylamine alcohol protein conjugate on the carrier glass fibre, anti-clenobuterol hydrochloride protein conjugate, anti-Ractopamine protein conjugate monoclonal antibody colloid gold label thing; Be distributed with one on the nitrocellulose membrane of middle part from back to front and be coated with nature controlling line (said forward and backward being meant with the diversion direction that the anti-mouse of rabbit resists more to the suction direction, before the diversion glass fibre is, the absorbent wool pulpboard is the back), article three, be coated with the detection line of salbutamol protein conjugate, clenobuterol hydrochloride protein conjugate, Ractopamine protein conjugate respectively, the nitrocellulose membrane two ends closely link to each other with absorbent wool pulpboard front end face with carrier glass fibre rear end face respectively, and carrier glass fibre front end face closely links to each other with diversion glass fibre rear end face.
In order to guarantee that the said overlay film of result of use is a pressure sensitive membrane.
It is to be noted; the utility model creative contribution is; with prior art through scientific and reasonable combination and configuration; constitute novel beta-stimulants albuterol, clenobuterol hydrochloride, Ractopamine ternary detection test bar; be effective to detect simultaneously beta-stimulants albuterol, clenobuterol hydrochloride, Ractopamine, the technical scheme that all that adopt structurally that equivalent replacement means have done and the utility model do not have essential distinction in essence all belongs to protection domain of the present utility model.
The utility model is through test, utilize this test-strips to test beta-stimulants albuterol, clenobuterol hydrochloride, Ractopamine simultaneously, only need an application of sample, can test beta-stimulants albuterol, clenobuterol hydrochloride, Ractopamine in 5-10 minute, on probation through 158 times, except that 1 example is indeterminate, all the other 157 examples have all reached promising result, rate of accuracy reached is more than 99%, therefore, it is simple that the present invention has method, convenient and swift, the characteristics that the result is accurate are the innovations in beta-stimulants albuterol, clenobuterol hydrochloride, the Ractopamine detection.
Claims (2)
1. beta-stimulants albuterol, clenobuterol hydrochloride, the Ractopamine ternary detection test bar, comprise base material PVC plate and overlay film, it is characterized in that, be disposed with diversion glass fibre (3) above the base material PVC plate (1) from front to back, carrier glass fibre (4), nitrocellulose membrane (2) and absorbent wool pulpboard (5), diversion glass fibre (3), carrier glass fibre (4) and be coated with the overlay film of forming by anterior coverlay (7) and rear portion coverlay (6) above the absorbent wool pulpboard (5), be distributed with one on the nitrocellulose membrane of middle part from back to front and be coated with the nature controlling line that the anti-mouse of rabbit resists more, article three, be coated with the salbutamol protein conjugate respectively, the clenobuterol hydrochloride protein conjugate, the detection line of Ractopamine protein conjugate, the nitrocellulose membrane two ends closely link to each other with absorbent wool pulpboard front end face with carrier glass fibre rear end face respectively, and carrier glass fibre front end face closely links to each other with diversion glass fibre rear end face.
2. beta-stimulants albuterol according to claim 1, clenobuterol hydrochloride, Ractopamine ternary detection test bar is characterized in that, said overlay film is a pressure sensitive membrane.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201020269898XU CN201828562U (en) | 2010-07-26 | 2010-07-26 | Test strip for salbutamol, clenbuterol and ractopamine ternary-detection of Beta-stimulant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201020269898XU CN201828562U (en) | 2010-07-26 | 2010-07-26 | Test strip for salbutamol, clenbuterol and ractopamine ternary-detection of Beta-stimulant |
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| Publication Number | Publication Date |
|---|---|
| CN201828562U true CN201828562U (en) | 2011-05-11 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201020269898XU Expired - Fee Related CN201828562U (en) | 2010-07-26 | 2010-07-26 | Test strip for salbutamol, clenbuterol and ractopamine ternary-detection of Beta-stimulant |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101915841A (en) * | 2010-07-26 | 2010-12-15 | 河南省科学院生物研究所有限责任公司 | Ternary detection test bar of beta-stimulants albuterol, clenobuterol hydrochloride and ractopamine and preparation method thereof |
| CN102967707A (en) * | 2012-03-30 | 2013-03-13 | 无锡福阳生物科技有限公司 | Triple immune colloidal gold rapid detection card and its preparation and use method |
| CN103163193A (en) * | 2013-03-06 | 2013-06-19 | 济南大学 | Preparation method of electrochemical sensor for detecting three clenbuterol hydrochloride and application |
| CN104311659A (en) * | 2014-10-22 | 2015-01-28 | 南京师范大学 | Multi-cluster antigen and wide-spectrum specific antibody of beta-adrenoceptor agonists and application of multi-cluster antigen and wide-spectrum specific antibody |
| CN104387467A (en) * | 2014-10-22 | 2015-03-04 | 南京师范大学 | Detection kit and detection paper for Beta-adrenergic receptor stimulant multiresidue detection |
-
2010
- 2010-07-26 CN CN201020269898XU patent/CN201828562U/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101915841A (en) * | 2010-07-26 | 2010-12-15 | 河南省科学院生物研究所有限责任公司 | Ternary detection test bar of beta-stimulants albuterol, clenobuterol hydrochloride and ractopamine and preparation method thereof |
| CN101915841B (en) * | 2010-07-26 | 2013-01-02 | 河南省科学院生物研究所有限责任公司 | Ternary detection test bar of beta-stimulants albuterol, clenobuterol hydrochloride and ractopamine and preparation method thereof |
| CN102967707A (en) * | 2012-03-30 | 2013-03-13 | 无锡福阳生物科技有限公司 | Triple immune colloidal gold rapid detection card and its preparation and use method |
| CN103163193A (en) * | 2013-03-06 | 2013-06-19 | 济南大学 | Preparation method of electrochemical sensor for detecting three clenbuterol hydrochloride and application |
| CN103163193B (en) * | 2013-03-06 | 2014-03-19 | 济南大学 | Preparation method of electrochemical sensor for detecting three clenbuterol hydrochloride and application |
| CN104311659A (en) * | 2014-10-22 | 2015-01-28 | 南京师范大学 | Multi-cluster antigen and wide-spectrum specific antibody of beta-adrenoceptor agonists and application of multi-cluster antigen and wide-spectrum specific antibody |
| CN104387467A (en) * | 2014-10-22 | 2015-03-04 | 南京师范大学 | Detection kit and detection paper for Beta-adrenergic receptor stimulant multiresidue detection |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20110511 Termination date: 20130726 |