CN201859136U - Array electrochemical sensor - Google Patents
Array electrochemical sensor Download PDFInfo
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- CN201859136U CN201859136U CN2010205863758U CN201020586375U CN201859136U CN 201859136 U CN201859136 U CN 201859136U CN 2010205863758 U CN2010205863758 U CN 2010205863758U CN 201020586375 U CN201020586375 U CN 201020586375U CN 201859136 U CN201859136 U CN 201859136U
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Abstract
The utility model discloses an array electrochemical sensor, which comprises a special printed circuit board made of epoxy glass cloth copper-clad laminates. A working area for loading liquor is arranged on the printed circuit board; 16 electrodes arranged in the working area includes a reference electrode, a counter electrode and 14 working electrodes; the reference electrode is a silver and silver chloride electrode; the counter electrode is a gold electrode; gold is coated on the surface of each working electrode; 16 lead wires formed by etching are further arranged on the printed circuit board; one end of each lead wire is connected with one electrode; and the other end of each lead wire is connected with an electric signal leading-out terminal on the back of the printed circuit board. The array electrochemical sensor can be applied to genetic polymorphism analysis, and has the advantages of simple structure and convenience in operation.
Description
Technical field
The utility model relates to a kind of electrochemical sensor, relates in particular to a kind of array electrochemical sensor that is applied to Polymorphism Analysis.
Background technology
Along with the progress of pharmacogenomics, find between the hereditary variability of body and the drug metabolism closely related to single nucleotide polymorphism and pharmacokinetics, pharmacodynamics correlation research.For example, the tacrolimus immunodepressant is mainly by liver and GI transport protein and system's metabolism of Cytochrome P450 isodynamic enzyme and decomposition.Research has confirmed that the required dosage of tacrolimus conspicuousness in CYP3A5*1/*1 and CYP3A5*1/*3 crowd will be higher than CYP3A5*3/*3 crowd, and promptly wild type or heterozygous CYP3A5 metabolism tacrolimus rapidly causes blood concentration decline.Therefore, before the decision organ transplant, the patient is carried out the CYP3A5 genetic analysis, determine metabolic type, adjust dosage, help the clinician to determine to use only immunodepressant combination and dosage, reach the optimization of dosage, subsidiary reaction and curative effect, realize that the individuation of immunodepressant is used.
Traditional Polymorphism Analysis method comprises restriction fragment length polymorphism (RFLP), single-strand conformation polymorphism (SSCP), denaturing gradient gel electrophoresis technology such as (DGGE), these technology must detect by gel electrophoresis, DNA chain secondary structure causes artificial false appearance easily, makes the result deviation occur.RFLP only can detect the SNP of restriction enzyme site, and no restriction enzyme site can not detect; And SSCP, DGGE spended time length, poor stability, sensitivity are limited, and these methods all are not widely adopted.And the electrochemical DNA sensor based on the allele-specific extension of exploitation in recent years in testing process, needs a plurality of reactions steps, is not easy to operation, has limited it in clinical applying.
The utility model content
Technical problem to be solved in the utility model is, a kind of array electrochemical sensor is provided, and is applied to Polymorphism Analysis, has simple in structure, easy to operate advantage.
For solving above technical matters, the utility model embodiment provides a kind of array electrochemical sensor, comprise the printed circuit board (PCB) of making by the epoxy glass fabric copper coated foil plate, described printed circuit board (PCB) is provided with the workspace of a carrying solution, have 16 electrodes in the described workspace, comprise that 1 contrast electrode, 1 are to electrode and 14 working electrodes; Described contrast electrode is a silver-silver chloride electrode, and described is gold electrode to electrode, and the surface of each working electrode is covered with gold;
Also have the lead-in wire that 16 etchings form on the described printed circuit board (PCB), every lead-in wire is corresponding with an electrode respectively; One end of every lead-in wire is connected with an electrode, and the other end leads to outside the described workspace.
Further, going back etching outside the workspace on the described printed circuit board (PCB) has 16 terminals, and each terminal is corresponding with a lead-in wire respectively; An end that leads to outside the workspace of every lead-in wire is connected with a terminal respectively; The top of described workspace also is covered with the film cover plate.
Wherein, described printed circuit board (PCB) is a double-sided printed-circuit board; Described workspace and described 16 electrodes are arranged on the upper surface of described printed circuit board (PCB); Described 16 lead-in wires and described 16 terminals are arranged on the lower surface of described printed circuit board (PCB); Described lead-in wire passes described printed circuit board (PCB), is connected with the electrode of the upper surface of described printed circuit board (PCB).
Described workspace is to dig the groove that forms at the upper surface of described printed circuit board (PCB); Described workspace be shaped as the polygon that circle, ellipse or turning are fillet; The volume of described workspace is greater than 150 microlitres.Described 16 terminals be evenly distributed in described workspace the bottom surface around; Described terminal is that diameter is the circular terminal of 0.5～5mm.
Further, also be provided with pilot hole on the described printed circuit board (PCB).
The array electrochemical sensor that the utility model embodiment provides, comprise the dedicated printed circuit board that adopts the epoxy glass fabric copper coated foil plate to make, this printed circuit board (PCB) is provided with the workspace that is used to carry testing sample and related solution, have in the workspace by 1 contrast electrode, 1 electrode and 14 16 channel sensor arrays that working electrode is formed, wherein, contrast electrode is a silver-silver chloride electrode, is gold electrode to electrode, and the surface of each working electrode is covered with gold.Behind the array electrochemical sensor combined with electrochemical analyser that the utility model provides, can be applicable to Polymorphism Analysis, it has simple in structure, easy to operate advantage.
Description of drawings
Fig. 1 is the structural representation of the array electrochemical sensor that provides of the utility model embodiment one;
Fig. 2 is the structural representation of the upper surface of array electrochemical sensor shown in Figure 1;
Fig. 3 is the structural representation of the lower surface of array electrochemical sensor shown in Figure 1;
Fig. 4 is the structural representation of the array electrochemical sensor that provides of the utility model embodiment two.
Embodiment
Below in conjunction with the accompanying drawing among the utility model embodiment, the technical scheme among the utility model embodiment is clearly and completely described.
Referring to Fig. 1, be the structural representation of the array electrochemical sensor that provides of the utility model embodiment one.
The array electrochemical sensor that present embodiment provides comprises the dedicated printed circuit board 1 of being made by the epoxy glass fabric copper coated foil plate, and printed circuit board (PCB) 1 is provided with the workspace 2 of a carrying solution.
Have 16 electrodes in the workspace 2, comprise that 1 contrast electrode, 1 to electrode and 14 working electrodes (contrast electrode 3 as shown in Figure 1, to electrode 4, working electrode 5), formed 16 channel sensor arrays.Wherein, contrast electrode 3 is a silver-silver chloride electrode, is gold electrode to electrode 4, and the surface of each working electrode is covered with gold.
Concrete, when making working electrode, the gold of high-frequency sputtering 800 dusts on working electrode (purity is 99.99%), and use the plasma instrument surface of working electrode is cleaned, improve the water wettability of working electrode surface and remove impurity, to improve the fixed efficiency of capture probe at working electrode surface.When making contrast electrode, conducting resinl (for example silver and epoxy resin composite) point sample to the electrode of printed circuit board (PCB), through after the high temperature drying, is promptly formed silver-silver chloride (Ag/AgCl) electrode after the energising of adding sodium perchlorate solution.
Further, also have the lead-in wire (lead-in wire 6 as shown in Figure 1) that 16 etchings form on the printed circuit board (PCB) 1, every lead-in wire is corresponding with an electrode respectively; One end of every lead-in wire is connected with an electrode, and the other end leads to outside the workspace.
Going back etching outside the workspace on the printed circuit board (PCB) 1 has 16 terminals (terminal 7 as shown in Figure 1), as the electric signal leading-out terminal.Each terminal is corresponding with a lead-in wire respectively, and an end that leads to outside the workspace of every lead-in wire is connected with a terminal respectively.For example, as shown in Figure 1, an end of lead-in wire 6 is connected with contrast electrode 3, and the other end of lead-in wire 6 leads to the outside of workspace 2, and is connected with the terminal 7 of 2 outsides, workspace.
Optionally, the top of workspace 2 also is covered with the film cover plate, can prevent that dust impurity from entering in the workspace, influences test result of samples.
During concrete enforcement, 16 lead-in wires, 16 terminals can be made on printed circuit board (PCB) by Graphics Application plating-etching method.
Preferably, printed circuit board (PCB) 1 is a double-sided printed-circuit board.Referring to Fig. 2, be the structural representation of the upper surface of the array electrochemical sensor that provides of present embodiment, workspace 2 and 16 electrodes are arranged on the upper surface (being the part of representing with solid line among Fig. 1) of printed circuit board (PCB) 1.Referring to Fig. 3, it is the structural representation of the lower surface of the array electrochemical sensor that provides of present embodiment, 16 lead-in wires and 16 terminals are arranged on the lower surface (part that promptly dots) of printed circuit board (PCB) 1 in Fig. 1, every lead-in wire all passes printed circuit board (PCB) 1, is connected with the electrode of the upper surface of this printed circuit board (PCB) 1.
During concrete enforcement, workspace 2 is to dig the groove that forms at the upper surface of printed circuit board (PCB) 1, workspace 2 be shaped as the polygon that circle, ellipse or turning are fillet, to reduce the zone of dead volume, help flowing of sample and related solution.Wherein, the volume of workspace 2 is greater than 150 microlitres.
16 terminals are arranged on the lower surface of printed circuit board (PCB) 1, and 16 terminals be evenly distributed in the workspace the outside around, this terminal is that diameter is the circular terminal of 0.5～5mm.
Because the workspace is to dig the groove that forms at the printed circuit board (PCB) upper surface, its bottom surface is very thin.Present embodiment 16 terminals are arranged on the workspace the outside around, not with the bottom surface overlaid of workspace, can improve the reliability of electrochemical sensor; And, also help the whole machine balancing of electrochemical sensor.
As Fig. 1～shown in Figure 3, be the structural representation of the array electrochemical sensor that provides of the utility model embodiment one, the shape of its workspace is that the turning is circular quadrilateral, and 16 terminals are arranged on the bottom surface of printed circuit board (PCB), and to divide equally be two rows, is respectively in the both sides of workspace.
Referring to Fig. 4, it is the structural representation of the array electrochemical sensor that provides of utility model embodiment two, the array electrochemical sensor of its structure and above-mentioned enforcement one is basic identical, difference is: the shape of workspace is oval, 16 terminals are arranged on the bottom surface of printed circuit board (PCB), and be evenly distributed in the workspace around.
In the middle of concrete enforcement, behind the array electrochemical sensor combined with electrochemical analyser (for example model is the electrochemical analyser of CHI-600B) that the utility model provides, can be applicable to Polymorphism Analysis.Wherein, testing sample and relevant solution are placed in the workspace of array electrochemical sensor, and 16 terminals on the array electrochemical sensor are connected with the thimble connector, and are connected with the electrode connecting line of electrochemical analyser by this thimble connector.
Further, as shown in Figure 1, on printed circuit board (PCB) 1, also be provided with pilot hole 8.This pilot hole can guarantee that 16 terminals are stable with the thimble connector, be connected reliably.
The array electrochemical sensor that the utility model embodiment provides, comprise the dedicated printed circuit board that adopts the epoxy glass fabric copper coated foil plate to make, this printed circuit board (PCB) is provided with the workspace that is used to carry testing sample and related solution, have in the workspace by 1 contrast electrode, 1 electrode and 14 16 channel sensor arrays that working electrode is formed, wherein, contrast electrode is a silver-silver chloride electrode, is gold electrode to electrode, and the surface of each working electrode is covered with gold.Behind the array electrochemical sensor combined with electrochemical analyser that the utility model provides, can be applicable to Polymorphism Analysis, it has simple in structure, easy to operate advantage.
The above is a preferred implementation of the present utility model; should be pointed out that for those skilled in the art, under the prerequisite that does not break away from the utility model principle; can also make some improvements and modifications, these improvements and modifications also are considered as protection domain of the present utility model.
Claims (10)
1. array electrochemical sensor, it is characterized in that, comprise the printed circuit board (PCB) of making by the epoxy glass fabric copper coated foil plate, described printed circuit board (PCB) is provided with the workspace of a carrying solution, have 16 electrodes in the described workspace, comprise that 1 contrast electrode, 1 are to electrode and 14 working electrodes; Described contrast electrode is a silver-silver chloride electrode, and described is gold electrode to electrode, and the surface of each working electrode is covered with gold;
Also have the lead-in wire that 16 etchings form on the described printed circuit board (PCB), every lead-in wire is corresponding with an electrode respectively; One end of every lead-in wire is connected with an electrode, and the other end leads to outside the described workspace.
2. array electrochemical sensor as claimed in claim 1 is characterized in that, going back etching outside the workspace on the described printed circuit board (PCB) has 16 terminals, and each terminal is corresponding with a lead-in wire respectively; An end that leads to outside the workspace of every lead-in wire is connected with a terminal respectively.
3. array electrochemical sensor as claimed in claim 2 is characterized in that the top of described workspace also is covered with the film cover plate.
4. array electrochemical sensor as claimed in claim 3 is characterized in that, described printed circuit board (PCB) is a double-sided printed-circuit board.
5. array electrochemical sensor as claimed in claim 4 is characterized in that, described workspace and described 16 electrodes are arranged on the upper surface of described printed circuit board (PCB).
6. array electrochemical sensor as claimed in claim 5 is characterized in that, described 16 lead-in wires and described 16 terminals are arranged on the lower surface of described printed circuit board (PCB); Described lead-in wire passes described printed circuit board (PCB), is connected with the electrode of the upper surface of described printed circuit board (PCB).
7. array electrochemical sensor as claimed in claim 6 is characterized in that, described workspace is to dig the groove that forms at the upper surface of described printed circuit board (PCB).
8. array electrochemical sensor as claimed in claim 7 is characterized in that, described workspace be shaped as the polygon that circle, ellipse or turning are fillet; The volume of described workspace is greater than 150 microlitres.
9. array electrochemical sensor as claimed in claim 8 is characterized in that, described 16 terminals be evenly distributed in the outside, described workspace around; Described terminal is that diameter is the circular terminal of 0.5～5mm.
10. as each described array electrochemical sensor of claim 1～9, it is characterized in that, also be provided with pilot hole on the described printed circuit board (PCB).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010205863758U CN201859136U (en) | 2010-10-29 | 2010-10-29 | Array electrochemical sensor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010205863758U CN201859136U (en) | 2010-10-29 | 2010-10-29 | Array electrochemical sensor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201859136U true CN201859136U (en) | 2011-06-08 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CN2010205863758U Expired - Fee Related CN201859136U (en) | 2010-10-29 | 2010-10-29 | Array electrochemical sensor |
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| CN (1) | CN201859136U (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103257163A (en) * | 2013-05-08 | 2013-08-21 | 中山大学达安基因股份有限公司 | Circuit board for gene detection |
| CN106546640A (en) * | 2015-09-16 | 2017-03-29 | 刘珊珊 | A kind of carrier for biosensor and preparation method thereof and application |
| CN109211992A (en) * | 2017-06-29 | 2019-01-15 | 深圳市理邦精密仪器股份有限公司 | The method of telegraph circuit plate and production electrode for vim and vigour Biochemistry measurement |
| CN116273760A (en) * | 2022-09-07 | 2023-06-23 | 中国飞机强度研究所 | Coating method of conductive sensor |
-
2010
- 2010-10-29 CN CN2010205863758U patent/CN201859136U/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103257163A (en) * | 2013-05-08 | 2013-08-21 | 中山大学达安基因股份有限公司 | Circuit board for gene detection |
| CN103257163B (en) * | 2013-05-08 | 2015-08-19 | 中山大学达安基因股份有限公司 | A kind of circuit board for genetic test |
| CN106546640A (en) * | 2015-09-16 | 2017-03-29 | 刘珊珊 | A kind of carrier for biosensor and preparation method thereof and application |
| CN109211992A (en) * | 2017-06-29 | 2019-01-15 | 深圳市理邦精密仪器股份有限公司 | The method of telegraph circuit plate and production electrode for vim and vigour Biochemistry measurement |
| CN116273760A (en) * | 2022-09-07 | 2023-06-23 | 中国飞机强度研究所 | Coating method of conductive sensor |
| CN116273760B (en) * | 2022-09-07 | 2024-02-23 | 中国飞机强度研究所 | Coating method of conductive sensor |
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Legal Events
| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20110608 Termination date: 20121029 |