CN108398472A - A kind of dual-port screen printing electrode having two-parameter detection function - Google Patents
A kind of dual-port screen printing electrode having two-parameter detection function Download PDFInfo
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- CN108398472A CN108398472A CN201810405393.2A CN201810405393A CN108398472A CN 108398472 A CN108398472 A CN 108398472A CN 201810405393 A CN201810405393 A CN 201810405393A CN 108398472 A CN108398472 A CN 108398472A
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- electrode
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- screen printing
- dual
- parameter detection
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
- G01N27/3272—Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
Abstract
The present invention relates to electrochemical method detection technique fields, provide a kind of dual-port screen printing electrode having two-parameter detection function, including electrode base board, electrode base board is equipped with detecting electrode component, detecting electrode component includes at least two electrode layers for being set up in parallel and extending along the length direction of electrode base board, it is covered with reaction enzyme layer above the middle part of part or all of electrode layer, the outside for reacting enzyme layer is equipped with lateral chromatography layer, lateral chromatography layer extends inwardly to the top of reaction enzyme layer, the stage casing of electrode layer is covered with insulating layer, insulating layer also covers the part of counter electrode layer on lateral chromatography layer, inserted terminal positioned at all electrode layer pins of the same end collectively constitutes an electrode ports.A kind of dual-port screen printing electrode response time having two-parameter detection function that the present invention makes is short, strong antijamming capability, the contrasting detection that can be carried out at the same time two groups of parameters, and the effective cost for reducing silk-screen printing reduces printing complexity.
Description
Technical field
The present invention relates to electrochemical technology fields, are specifically related to a kind of dual-port silk screen having two-parameter detection function
It prints electrode.
Background technology
It is vital that small biological molecule can be rapidly and sensitively detected in clinical diagnostic applications.Wherein, blood glucose, Portugal
The detection of grape sugar and uric acid is particularly significant.Blood glucose value directly reflects that the degree of practical carbohydrate metabolism disturbance, glycemic control refer to whole day
The overall control of blood glucose；Glucose is indispensable substance in vital movement, it can directly participate in metabolism in human body
Process；Uric acid (UA) is present in blood and urine, and higher or lower uric acid level may lead to disease.Thus for this three
The Fast Detection Technique of kind molecule causes extensive concern.Electrochemical methods be considered as a kind of detection blood glucose, glucose,
The potential method of uric acid because showed in electrode surface these molecules it is electroactive, easily by redox.
Mainly there are enzyme coupled assay and high performance liquid chromatography, but high performance liquid chromatography in both methods at present
Detect relatively complicated, cost is higher, and existing enzyme coupled assay is less, mostly uses single electrochemical electrode test, such
Electrochemical electrode limitation is bigger, and detection object is single, and cost is higher and anti-interference ability is poor.
In the biological sample of human body, coexist high concentration many kinds of substance have with its extremely similar electrochemical properties,
Necessarily cause to interfere accordingly during measurement.So developing, at low cost, manufacture craft is simple, highly sensitive, Gao Xuan
The electrochemical sensor for measuring blood glucose, glucose, uric acid of selecting property is as people's urgent problem to be solved..
Invention content
The technical problem to be solved by the present invention is to：In view of the deficienciess of the prior art, provide one kind can simultaneously into
The contrasting detection of two groups of parameters of row, the effective cost for reducing silk-screen printing, reduce printing complexity has two-parameter detection
The dual-port screen printing electrode of function.
In order to solve the above technical problems, the technical scheme is that：A kind of dual-port having two-parameter detection function
Screen printing electrode, includes the electrode base board of insulation, and the electrode base board is equipped with detecting electrode component, the detecting electrode group
Part includes at least two and is set up in parallel and along the electrode layer that the length direction of the electrode base board extends, the part or all of electricity
It is covered with reaction enzyme layer above the middle part of pole layer, the outside of the reaction enzyme layer is equipped with lateral chromatography layer, in the electrode layer
Section is covered with insulating layer, and the insulating layer also covers the part that the electrode layer is corresponded on the lateral chromatography layer, is located at same
The inserted terminal of all electrode layer pins at end collectively constitutes an electrode ports.
Preferably, the quantity for being covered with the electrode layer of the reaction enzyme layer is two or more, all electrode layers
On the reaction enzyme layer ingredient it is not all the same.
Preferably, the quantity for being covered with the electrode layer of the reaction enzyme layer is two or more, all electrode layers
On the reaction enzyme layer ingredient all same.
Preferably, the reaction enzyme layer is two or three in glucose oxidase, hexokinase and urate oxidase.
Preferably, the reaction enzyme layer is one kind in glucose oxidase or hexokinase or urate oxidase.
Preferably, the lateral chromatography layer is the hydrophilic material that can form siphon passage.
Preferably, the hydrophilic material is water-insoluble polysaccharide polymer, hydrophily chromatographic resin and macropore polypropylene
One kind in acid esters.
Preferably, the electrode layer is the mixture of one or both of conductive carbon paste and conductive silver paste.
Preferably, the insulating layer is dielectric ink.
Preferably, the tow sides of the electrode base board are equipped with the detecting electrode component.
Preferably, the middle part of the electrode base board is equipped with water board, and the water board is with by hydrophilic plant fibre
Dimension is made, and through the tow sides of the electrode base board.
By adopting the above-described technical solution, the beneficial effects of the invention are as follows：
The lateral chromatography layer of the dual-port screen printing electrode provided by the invention for having two-parameter detection function can incite somebody to action
Detection stoste chromatography is guided at reaction enzyme layer, and to obtain more accurate testing result, the electrode layer that electrode base board is equipped with is used
It can be used for quickly detecting to obtain two groups of testing results for same ingredient to be measured in two electrode ports of conduction, setting,
Convenient for comparative analysis detection data, the accuracy of testing result is further increased.
Reaction enzyme layer can be chemically reacted with the ingredient to be measured in detection stoste, induced current be generated, by detection
The electric current is simultaneously analyzed, you can obtains the concentration of ingredient to be measured, the convenient and efficient response time is short, when reaction enzyme layer is glucose
When oxidizing ferment, the concentration of glucose can detect；When it is hexokinase to react enzyme layer, blood glucose (including glucose, sweet dew can detect
Sugar, Glucosamine and fructose) concentration；When it is urate oxidase to react enzyme layer, the concentration of uric acid can detect.
When the reaction enzymes composition of layer difference on electrode layer, this has the dual-port silk-screen printing electricity of two-parameter detection function
Pole then can be carried out at the same time detection to ingredient to be measured different in detection stoste, increase it and detect diversity, improve detection efficiency.
Lateral chromatography layer is guided to reaction enzyme layer for receiving detection stoste and being chromatographed, poly- using water-insoluble polysaccharide
Close lateral chromatography layer made of a kind of hydrophilic material in object, hydrophily chromatographic resin and macropore polyacrylate, then it can shape
At siphon passage, preferably chromatography water conservancy diversion effect is obtained；
Electrode layer made of mixture using one or both of conductive carbon paste and conductive silver paste, then so that electrode layer
With excellent electric conductivity.
It is respectively provided with detecting electrode component in the tow sides of electrode base board, the two-sided use of electrode base board may be implemented, is increased
Add this to have the access times of the dual-port screen printing electrode of anti-interference function, reduces the waste of electrode base board consumptive material, simultaneously
Detection efficiency is improved, printing cost is reduced.
The dual-port screen printing electrode response time provided by the invention for having two-parameter detection function is short, anti-interference energy
Power is strong, can be carried out at the same time the contrasting detection of two groups of parameters, and the effective cost for reducing silk-screen printing reduces printing complexity.
Description of the drawings
Fig. 1 is that the structure vertical view of the dual-port screen printing electrode for having two-parameter detection function of the embodiment of the present invention is shown
It is intended to；
Fig. 2 is the A-A schematic cross-sectional views for the dual-port screen printing electrode for having two-parameter detection function shown in Fig. 1；
Fig. 3 is the B-B schematic cross-sectional views for the dual-port screen printing electrode for having two-parameter detection function shown in Fig. 1；
In figure：1- electrode base boards, 21- first electrodes port, 22- second electrodes port, 3- lateral chromatography layers, 4- insulation
Layer, 51- first electrodes, 52- second electrodes, 6- react enzyme layer, 7- water boards.
Specific implementation mode
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to the accompanying drawings and embodiments, right
The present invention is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, and
It is not used in the restriction present invention.
Fig. 1-Fig. 3 collectively illustrates the dual-port screen printing electrode provided by the invention for having two-parameter detection function
Structural schematic diagram, for convenience of description, this figure only provide structure division related to the present invention.
Have the dual-port screen printing electrode of two-parameter detection function, includes the electrode base board 1 of insulation, electrode base board 1
For carrying electrode other structures layer；Electrode base board 1 be equipped with detecting electrode component, detecting electrode component include at least two simultaneously
The electrode layer they row setting and extended along the length direction of electrode base board 1, electrode layer play conductive effect, in order to make it easy to understand,
By taking two electrode layers as an example, two electrode layers are respectively first electrode 51 and second electrode 52；It is equal above the middle part of two electrode layers
It is covered with reaction enzyme layer 6, reaction enzyme layer 6 is used to chemically react with the ingredient to be measured in detection stoste, generates induced current,
It detects the electric current and carries out the concentration that analysis can be obtained ingredient to be measured, the convenient and efficient and response time is short；
When the ingredient difference of first electrode 51 and the reaction enzyme layer 6 in second electrode 52, this has two-parameter detection work(
The dual-port screen printing electrode of energy then can be carried out at the same time detection to ingredient to be measured different in detection stoste, increase its detection
Diversity improves detection efficiency；
The outside for reacting enzyme layer 6 is equipped with lateral chromatography layer 3, and lateral chromatography layer 3 is used to receive detection stoste, and will detection original
Liquid layer analysis is guided in reaction enzyme layer 6 and is chemically reacted for it, generates response current；In this embodiment, select water insoluble
Lateral chromatography layer made of a kind of hydrophilic material in property polysaccharide polymer, hydrophily chromatographic resin and macropore polyacrylate
3, then siphon passage can be formed, preferably chromatography water conservancy diversion effect is obtained.
The stage casing of electrode layer is covered with insulating layer 4, and insulating layer 4 also covers counter electrode layer on lateral chromatography layer 3
Part then can effectively avoid detection stoste and accidentally be dripped or immerse 51 He of first electrode without lateral chromatography layer 3 and reaction enzyme layer 6
Second electrode 52 influences the accuracy of testing result, and the inserted terminal positioned at all electrode layer pins of the same end collectively constitutes one
A electrode ports add up to two electrode ports, that is, first electrode port 21 and second electrode port 22, can be directed to same to be measured
Ingredient is used for quickly detecting to obtain two groups of testing results, is convenient for comparative analysis detection data, further increases the standard of testing result
True property.
Electrode layer made of mixture using one or both of conductive carbon paste and conductive silver paste, then so that electrode layer
With excellent electric conductivity.
It is respectively provided with above-mentioned detecting electrode component in the tow sides of electrode base board 1, the two-sided of electrode base board 1, which may be implemented, to be made
With increasing the access times of the dual-port screen printing electrode for having anti-interference function, reduce the wave of 1 consumptive material of electrode base board
Take, while improving detection efficiency, reduces printing cost.
The middle part of electrode base board 1 is equipped with water board 7, and water board 7 is made of hydrophilic plant fiber, and runs through institute
The tow sides for stating electrode base board 1 are located at 4 inside of insulating layer, and water board 7 can effectively adsorb detection stoste, and lateral
Under the siphonage for chromatographing layer 3, reacting between the reaction enzyme layer 6 of tow sides and detection stoste is realized, it is anti-dry to realize
The tow sides detection for disturbing dual-port screen printing electrode does not have to that detection stoste is added dropwise repeatedly, and operation is more convenient.
Following embodiment be by two electrode layers have the dual-port screen printing electrode of two-parameter detection function for, needle
To illustrating for the different situations of the reaction enzymes used on each electrode layer：
Embodiment 1
When first electrode 51 is identical with the ingredient of the reaction enzyme layer 6 in second electrode 52, reaction enzyme layer 6 is that grape is glycoxidative
When enzyme, operation principle is as follows：
(1) it takes detection stoste one to drip, instills lateral chromatography layer 3, detection chromatography water conservancy diversion of the stoste through lateral chromatography layer 3 is laggard
Enter to react enzyme layer 6, contacts and chemically react with glucose oxidase；
(2) first electrode port 21 is detected by electrochemical workstation to meet with a response electric current I1；
(3) second electrode port 22 is detected by electrochemical workstation to meet with a response electric current I2；
(4) get response current difference I=by being made the difference to two response currents | I1-I2|。
Response current I1And I2The electric current of generation, warp are reacted with glucose oxidase by glucose in same detection stoste
It crosses analysis and can be calculated two groups of detection datas, response current difference I represents the testing result of concentration of glucose in detection stoste
Accuracy, I values are smaller, and accuracy is higher, and I values are smaller, i.e., this testing result is more accurate.
Similarly, identical with the ingredient of the reaction enzyme layer 6 in second electrode 52 when first electrode 51, reaction enzyme layer 6 is hexose
When kinases or urate oxidase, detection can be corresponded to and calculate blood glucose (including glucose, mannose, aminoglucose in detection stoste
Sugar and fructose) or uric acid concentration and testing result comparison accuracy.
Embodiment 2
When the ingredient of the reaction enzyme layer 6 in first electrode 51 and second electrode 52 is all different, the reaction of first electrode 51
Enzyme layer 6 is glucose oxidase, and when the reaction enzyme layer 6 in second electrode 52 is urate oxidase, operation principle is as follows：
(1) it takes detection stoste one to drip, instills lateral chromatography layer 3, detection chromatography water conservancy diversion of the stoste through lateral chromatography layer 3 is laggard
Enter to react enzyme layer 6, contacts and chemically react with glucose oxidase；
(2) first electrode port 21 is detected by electrochemical workstation (by the external connection end of the pin of first electrode 51 to be applied
Voltage) meet with a response electric current I1；
(3) second electrode port 22 is detected by electrochemical workstation (by the external connection end of the pin of second electrode 52 to be applied
Voltage) meet with a response electric current I2；
Electric current I1 is the response current for detecting glucose in stoste, electric current I2To detect the response current of uric acid in stoste,
The concentration that can obtain glucose and uric acid in detection stoste respectively is calculated by analyzing.
So, it can be achieved that this has the dual-port screen printing electrode of two-parameter detection function to the grape in detection stoste
It is detected while sugar and uric acid concentration, is effectively improved detection efficiency, reduce testing cost.
Similarly, when the reaction enzyme layer 6 in first electrode 51 and second electrode 52, other are anti-at respectively two different
When answering enzyme, the concentration of two kinds of difference ingredients to be measured can be also detected simultaneously.
The dual-port screen printing electrode response time provided by the invention for having two-parameter detection function is short, anti-interference energy
Power is strong, can be carried out at the same time the contrasting detection of two groups of parameters, and the effective cost for reducing silk-screen printing reduces printing complexity.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
All any modification, equivalent and improvement etc., should all be included in the protection scope of the present invention made by within refreshing and principle.
Claims (10)
1. a kind of dual-port screen printing electrode having two-parameter detection function, includes the electrode base board of insulation, feature exists
In:The electrode base board is equipped with detecting electrode component, and the detecting electrode component is set up in parallel and including at least two along institute
The electrode layer that the length direction of electrode base board extends is stated, reaction enzymes are covered with above the middle part of the part or all of electrode layer
The outside of layer, the reaction enzyme layer is equipped with lateral chromatography layer, and the stage casing of the electrode layer is covered with insulating layer, and the insulating layer is also
The part for corresponding to the electrode layer on the lateral chromatography layer is covered, the grafting of all electrode layer pins of the same end is located at
End collectively constitutes an electrode ports.
2. having the dual-port screen printing electrode of two-parameter detection function as described in claim 1, it is characterised in that：It is covered with
The quantity of the electrode layer of the reaction enzyme layer is two or more, the ingredient of the reaction enzyme layer on all electrode layers
It is not all the same.
3. as described in claim 1 it is anti-interference can two-parameter detection dual-port screen printing electrode, it is characterised in that：It is covered with
The quantity of the electrode layer of the reaction enzyme layer is two or more, the ingredient of the reaction enzyme layer on all electrode layers
All same.
4. having the dual-port screen printing electrode of two-parameter detection function as claimed in claim 2, it is characterised in that：It is described anti-
It is two or three in glucose oxidase, hexokinase and urate oxidase to answer enzyme layer.
5. having the dual-port screen printing electrode of two-parameter detection function as claimed in claim 3, it is characterised in that：It is described anti-
It is one kind in glucose oxidase or hexokinase or urate oxidase to answer enzyme layer.
6. having the dual-port screen printing electrode of two-parameter detection function as described in claim 1, it is characterised in that：It is described
Lateral chromatography layer is the hydrophilic material that can form siphon passage, and the hydrophilic material is water-insoluble polysaccharide polymer, parent
One kind in aqueous chromatographic resin and macropore polyacrylate.
7. having the dual-port screen printing electrode of two-parameter detection function as described in claim 1, it is characterised in that：The electricity
Pole layer is the mixture of one or both of conductive carbon paste and conductive silver paste.
8. having the dual-port screen printing electrode of two-parameter detection function as described in claim 1, it is characterised in that：It is described exhausted
Edge layer is dielectric ink.
9. as claim 1 to 8 any one of them has the dual-port screen printing electrode of two-parameter detection function, feature
It is：The tow sides of the electrode base board are equipped with the detecting electrode component.
10. having the dual-port screen printing electrode of two-parameter detection function as claimed in claim 9, it is characterised in that：It is described
The middle part of electrode base board is equipped with water board, and the water board is made of hydrophilic plant fiber, and runs through the electrode
The tow sides of substrate.
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| CN201810405393.2A CN108398472A (en) | 2018-04-29 | 2018-04-29 | A kind of dual-port screen printing electrode having two-parameter detection function |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109358101A (en) * | 2018-08-31 | 2019-02-19 | 浙江大学山东工业技术研究院 | A kind of electrochemical sensor electrodes test piece of multi-parameter detection |
| CN110146568A (en) * | 2018-11-29 | 2019-08-20 | 浙江大学山东工业技术研究院 | A kind of multi-parameter electrochemical detection electrode piece |
| CN110146562A (en) * | 2018-12-17 | 2019-08-20 | 浙江大学山东工业技术研究院 | It is a kind of based on Prussian blue no enzymuria acid sensor and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104749228A (en) * | 2013-12-30 | 2015-07-01 | 深圳先进技术研究院 | Test strip and method for detecting blood glucose and blood lipid simultaneously |
| CN208076447U (en) * | 2018-04-29 | 2018-11-09 | 浙江大学山东工业技术研究院 | A kind of dual-port screen printing electrode having two-parameter detection function |
-
2018
- 2018-04-29 CN CN201810405393.2A patent/CN108398472A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104749228A (en) * | 2013-12-30 | 2015-07-01 | 深圳先进技术研究院 | Test strip and method for detecting blood glucose and blood lipid simultaneously |
| CN208076447U (en) * | 2018-04-29 | 2018-11-09 | 浙江大学山东工业技术研究院 | A kind of dual-port screen printing electrode having two-parameter detection function |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109358101A (en) * | 2018-08-31 | 2019-02-19 | 浙江大学山东工业技术研究院 | A kind of electrochemical sensor electrodes test piece of multi-parameter detection |
| CN110146568A (en) * | 2018-11-29 | 2019-08-20 | 浙江大学山东工业技术研究院 | A kind of multi-parameter electrochemical detection electrode piece |
| CN110146562A (en) * | 2018-12-17 | 2019-08-20 | 浙江大学山东工业技术研究院 | It is a kind of based on Prussian blue no enzymuria acid sensor and preparation method thereof |
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