CN203555741U - Microelectrode of micron-scale glucose sensor - Google Patents
Microelectrode of micron-scale glucose sensor Download PDFInfo
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- CN203555741U CN203555741U CN201320570566.9U CN201320570566U CN203555741U CN 203555741 U CN203555741 U CN 203555741U CN 201320570566 U CN201320570566 U CN 201320570566U CN 203555741 U CN203555741 U CN 203555741U
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Abstract
The utility model relates to the field of detection devices, particularly to a microelectrode of a micron-scale glucose sensor. The microelectrode comprises a base, an operating electrode and a counter electrode. The front part of the base is an operating area. The operating electrode and the counter electrode are disposed in the operating area of the base. The largest diameter of the cross section of the operating area is 1 mm or less. The tail part of the base is provided with PADs corresponding to all the electrodes one by one. The operating electrode and the counter electrode are connected with the corresponding PADs through lead wires. The microelectrode allows the electrodes to be properly distributed on the flat or cylindrical insulation base, restricts the width of the electrodes or the diameter of the cross section of a cylindrical electrode, so that size of the electrodes is effectively reduced, and then rejection reaction of an acceptor is reduced.
Description
Technical field
This utility model relates to detecting instrument field, particularly relates to a kind of micro-meter scale glucose sensor microelectrode.
Background technology
First kind tradition blood sugar monitoring instrument is to have wound gauge, its method is that finger tip blood is measured check, Equations of The Second Kind is non-invasive blood-sugar detecting instrument, comprise subcutaneous tissue interstitial fluid glucose detection instrument and spectrum analysis blood glucose meter, the 3rd class is continous way blood sugar monitoring instrument, conventionally such blood glucose meter is by add a glucose sensor at the front end of fine needle head, implant subcutaneous tissue, utilize the consumption of oxygen or the generation of hydroxyl ion in contained glucose oxidase enzymatic determination subcutaneous tissue interstitial fluid, convert whereby dextrose equivalent to.
In continuous blood sugar measuring system, glucose sensor is vital, research shows, it is feasible by implantable glucose sensor, being undertaken in body glucose continuous detecting, consider the facility of use, safety and reliability, the implantation of sensor should be chosen in subcutaneous sensor that can continuous measurement chemical composition for implantable bioartificial body, and must meet its corresponding organism characteristic, these characteristics are all relevant with the bio-compatibility of sensor, in the situation that having implant to implant, biological tissue or organ all can reflect in a organized way with receptor and reflect, this has special selectivity with regard to meaning the chemicals that sensor not only will have pair need to detect, and after implanting tissue, in relatively long time, there is good stability and be good response time properties to the fast-changing power of test of measured object concentration.
The basis that electrode system detects as sensor, has determined to a great extent the performance of sensor and has detected effect.The electrode of the traditional size of in the past using is because volume is relatively large, during implanting, larger to the zest of tissue, causes more serious rejection, changes test environment, and then affects the testing result of electrode.Therefore, adopt the electrode system of small size, become the trend of development, when reducing electrode volume, usually because of the reduction of electrode effective area, cause that electrical signal intensity is lower, in order to obtain higher detection sensitivity, need to carry out modification to electrode surface, improve response active, the determinand of realizing under low concentration detects
Utility model content
The shortcoming of prior art in view of the above, the purpose of this utility model is to provide a kind of micro-meter scale glucose sensor microelectrode, for solving the problems of the prior art.This utility model is by each electrode that reasonably distributes in the dielectric base of plane or column, limits the width of electrode or the diameter of section of columnar electrode effectively reduces electrode volume simultaneously, thereby reduces the rejection of receptor.And further at electrode face finish electroactive material, improving the response performance of electrode as CNT and nanometer gold.
For achieving the above object and other relevant objects, this utility model provides a kind of micro-meter scale glucose sensor microelectrode, comprise substrate, working electrode, to electrode, the front portion of described substrate is working region, described working electrode, electrode is positioned on the working region of described substrate, maximum gauge≤1 millimeter of the cross section of described working region, the afterbody of described substrate is provided with and each electrode PAD(pin one to one), described working electrode, electrode is all connected by the wire PAD corresponding with it.
Preferably, described micro-meter scale glucose sensor microelectrode is two plane type structure, the working region of its substrate is plate, also comprise reference electrode, described electrode and reference electrode are positioned in a plane of working region of substrate, working electrode is positioned in another plane of working region of substrate, is reference electrode 3 times to the area of electrode.Can front and back or placed side by side in surface, working region to electrode and reference electrode.
Preferred, also comprise auxiliary electrode, described auxiliary electrode and working electrode are in the same plane, and the area of auxiliary electrode and working electrode equates.Described auxiliary electrode also connects by the wire PAD corresponding with it.Auxiliary electrode and working electrode can front and back or placed side by side in surface, working region.
Two plane type microelectrode makes full use of the upper and lower surface of the working region of substrate, and effective area is larger, and signal sensitivity is higher.
Preferably, described each electrode is rectangle, and the width of each electrode is 0.01～1 millimeter.
Preferred, the width of working region is corresponding with the overall width of electrode.
Preferably, the area of described each electrode is 0.1-2mm
2.
Preferably, dielectric base thickness is 0.01～0.8 millimeter.
Preferably, described micro-meter scale glucose sensor microelectrode is notch cuttype multiple structure, also comprise reference electrode, the first insulating barrier and the second insulating barrier, described the first insulating barrier is positioned in described substrate, described the first insulating barrier be shorter in length than substrate, described the second insulating barrier is positioned on the first insulating barrier, described the second insulating barrier be shorter in length than the first insulating barrier, described the first insulating barrier, the second insulating barrier, the afterbody of substrate overlaps, described the first insulating barrier, the second insulating barrier, the front portion of substrate forms the three-decker of notch cuttype, described working electrode, reference electrode and electrode being laid respectively in the Different Plane of three-decker of notch cuttype.
Preferably, described each electrode is rectangle, and the width of each electrode is 0.01～1 millimeter.
Preferably, the area of described each electrode is 0.1-2mm
2.
Preferably, the thickness of dielectric base, the first insulating barrier, the second insulating barrier is 0.01～0.8 millimeter; Be preferably 0.01-0.2 millimeter.
Working electrode, electrode and reference electrode are distributed in respectively on substrate and insulating barrier, are exposed in external environment condition.Each electrode is connected with interface PAD by the golden wire distributing in one plane; a part and the electrode contact of gold wire; and guidewire body part is positioned under insulating barrier, under preferable case, golden wire is placed under insulating barrier, effectively guardwire part., each electrode is distributed in Different Plane meanwhile, on the one hand the spacing of electrode is widened, reduced micro-environment impact each other, stair-stepping distribution of electrodes can effectively suppress the interference of human response to electrode response simultaneously; On the other hand, distribution of electrodes, in Different Plane, under the constant prerequisite of each electrode effective area, can further be reduced to the width of whole electrode.The electrode overall width of multiple field microelectrode is expected to reduce half left and right on plane formula microelectrode basis.
Preferably, described micro-meter scale glucose sensor microelectrode is circulus, and the working region of its substrate is column type or taper, also comprises reference electrode, each electrode all with annular distribution on working region.
Preferred, also comprise auxiliary electrode, described auxiliary electrode also with annular distribution on working region.
Preferred, when the working region of substrate is column type, the diameter 0.1-1 millimeter of column type; Be preferably 0.2-0.8 millimeter.
Preferably, the area of described each electrode is 0.1-10mm
2.
The level and smooth shapes of substrates of microelectrode of circulus has reduced the sharpened edge of plane electrode to the zest of tissue, is conducive to reduce human body rejection, realizes implanted and detects for a long time.
Preferably, described micro-meter scale glucose sensor microelectrode is helical structure, the working region of its substrate is column type or taper, also comprise reference electrode, described working electrode, to any one in electrode and reference electrode, be thread, and with spiral form uniform winding around the working region of substrate, all the other two electrodes with annular distribution on working region.
Preferably, described micro-meter scale glucose sensor microelectrode is helical structure, the working region of its substrate is column type or taper, also comprise reference electrode and auxiliary electrode, by working electrode, to any one in electrode, auxiliary electrode and reference electrode, be thread, and with spiral form uniform winding around the working region of substrate, its excess-three electrode with annular distribution on working region.
Preferably, the area of described each electrode is 0.1-10mm
2.
Preferred, the internal diameter of described bung flange is 0.2～1 millimeter, and the diameter of the working region of described substrate is >=0.1mm, and < 1mm.
Preferred, by reference electrode, be thread, and with spiral form uniform winding around the working region of substrate, remaining electrode with annular distribution on working region, the position of reference electrode is corresponding with the position of remaining electrode, and reference electrode does not contact with remaining electrode.
Helical form microelectrode is wrapped in electrode around dielectric base with helical form, greatly increases electrode reaction area, contributes to improve current-responsive intensity.
Preferably, described wire is golden wire, and is all positioned at described substrate inside.
In this utility model of described micro-meter scale glucose sensor microelectrode, " microelectrode " is defined as size on bidimensional cross section or diameter within the scope of 0.01～1 millimeter.Length for microelectrode does not further limit.
Preferably, the total length of described micro-meter scale glucose sensor microelectrode is 0.5-8cm; Be preferably 1.5-4.5cm.
The substrate that this utility model is used is the material with excellent insulating properties, mainly from inorganic non-metallic pottery, silica glass and organic polymer etc., consider simultaneously and the applied environment of implanted electrode require base material to have high impermeability and high mechanical properties.
Preferably, the material of described substrate is selected from one or more the combination in politef (Teflon), polyethylene (PE), polrvinyl chloride (PVC), acrylonitrile-butadiene-styrene copolymer (ABS), polymethyl methacrylate (PMMA), Merlon (PC), polyimides (PI) etc.
For working electrode with to electrode, this utility model adopts the material with satisfactory electrical conductivity and extensive chemical inertia as electrode material.
Preferably, described electrode is selected to the one in graphite electrode, glass-carbon electrode, noble metal electrode etc.
Preferably, described working electrode is selected from the one in graphite electrode, glass-carbon electrode, noble metal electrode etc.
Consider the requirement of the stability of good ductility and surface texture, noble metal electrode becomes good selection as gold electrode, platinum electrode, silver electrode etc.
Further preferred, described working electrode and electrode is to platinum electrode.
Preferably, described reference electrode is selected from the one in silver chloride/silver electrode or calomel electrode.
Preferably, the thickness of described each electrode is 0.1～200 nanometer; Be preferably 1～10 nanometer.
Preferably, described each electrode surface is provided with carbon nanotube layer decorative layer.Utilize the distinctive mechanical strength of CNT, high-specific surface area, swift electron transmission effects and chemical stability, at in type electrode surface, by physical absorption, embedding or covalent bond and etc. mode, by the carbon nano tube modified electrode surface that arrives, to improve electronics transmission speed, the while is because its specific surface area can be served as greatly a kind of good catalyst (enzyme) carrier.Described carbon nanotube layer decorative layer can be fixed on electrode surface by Nafion Solution Dispersion method, covalency fixation etc.
Preferably, described electrode surface is also provided with enzyme decorative layer.Described enzyme (being preferably glucoseoxidase) decorative layer can be fixed on electrode surface by poly-D-lysine.
Preferably, described each electrode surface is provided with decorated by nano-gold layer.Electrode face finish of the present utility model can also be the active characteristic of utilizing the common capacitive of biology that nanometer gold is good and can not destroying organism endoenzyme and protein, at in type electrode surface, by modes such as covalent cross-linkings, decorated by nano-gold is arrived to electrode surface.Described decorated by nano-gold layer can be fixed on electrode surface by electroreduction method, sol-gel process etc.
Microelectrode system in this utility model can be divided into three-electrode system and two electrode systems, wherein three-electrode system be one to electrode, a reference electrode and at least one working electrode, two electrode systems be one to electrode and at least one working electrode.In addition, this utility model, according to the quantity of working electrode, also can be divided into two kinds of situations: 1) single working electrode: working electrode only has one; 2) dual-electrode: working electrode has two, wherein one is reduced with material generation electroxidation to be detected, produces the signal of telecommunication, is called " working electrode "; Another root is responsible for detecting the response signal of chaff interference or background solution conventionally, is referred to as " auxiliary electrode ".Above-mentioned various electrode building form all has its unique advantage, and wherein three-electrode system, because have more one with reference electrode, therefore can effectively be controlled detection current potential, prevents the situation of potential drifting; Two electrode systems structure are simpler, and cost of manufacture is lower.
Accompanying drawing explanation
Fig. 1 is the front elevation of the two plane type microelectrode top view of this utility model micro-meter scale glucose sensor microelectrode
Fig. 2 is the back view of the two plane type microelectrode top view of this utility model micro-meter scale glucose sensor microelectrode.
Fig. 3 is the top view of the multiple field microelectrode of this utility model micro-meter scale glucose sensor microelectrode.
Fig. 4 is the side view of the multiple field microelectrode of this utility model micro-meter scale glucose sensor microelectrode.
Fig. 5 is the top view of the ring-type microelectrode of this utility model micro-meter scale glucose sensor microelectrode.
Fig. 6 is the top view of the helical form microelectrode of this utility model micro-meter scale glucose sensor microelectrode.
Element numbers explanation
The specific embodiment
By specific instantiation, embodiment of the present utility model is described below, those skilled in the art can understand other advantages of the present utility model and effect easily by the disclosed content of this description.This utility model can also be implemented or be applied by the other different specific embodiment, and the every details in this description also can be based on different viewpoints and application, carries out various modifications or change not deviating under spirit of the present utility model.
Notice, process equipment or device concrete not dated in the following example all adopt conventional equipment or the device in this area; All force value and scope all refer to absolute pressure.
In addition should be understood that one or more method steps of mentioning in this utility model do not repel between the step that can also have additive method step or clearly mention at these before and after described combination step can also insert additive method step, except as otherwise noted; Will also be understood that, the relation that is connected between one or more equipment/devices of mentioning in this utility model is not repelled between two equipment/devices that can also have other equipment/devices or clearly mention at these before and after described unit equipment/device can also insert other equipment/devices, except as otherwise noted.And, except as otherwise noted, the numbering of various method steps is only for differentiating the convenient tool of various method steps, but not for limiting the ordering of various method steps or limiting the enforceable scope of this utility model, the change of its relativeness or adjustment, in the situation that changing technology contents without essence, when being also considered as the enforceable category of this utility model.
Embodiment 1
Two plane type microelectrode
The two plane type microelectrode of the microelectrode of micro-meter scale glucose sensor shown in Fig. 1 and Fig. 2, substrate 14 thickness are 0.01-0.8mm, and each electrode is rectangle, and the width of each electrode is 0.01-1mm, and microelectrode total length is 0.5-8cm, the area of each electrode is 0.1-2mm
2electrode 17 and reference electrode 11 are positioned on a face of substrate 14, working electrode 18 and auxiliary electrode 19 are positioned on another face of substrate 14, each electrode is connected on gold PAD15 processed by golden wire 16, and PAD15 is corresponding one by one with each electrode, to the area of electrode 17, be 3 times of reference electrode 11, the area of auxiliary electrode 19 and working electrode 18 equates.
Embodiment 2
Multiple field microelectrode
The multiple field microelectrode of the microelectrode of micro-meter scale glucose sensor shown in Fig. 3 and Fig. 4, substrate 21 is provided with the first insulating barrier and the second insulating barrier, and form notch cuttype, the thickness of substrate 21, the first insulating barrier, the second insulating barrier is 0.01-0.8mm, each electrode is rectangle, the width of each electrode is 0.01-1mm, and microelectrode total length is 0.5-8cm, and the area of each electrode is 0.1-2mm
2, electrode 24, working electrode 25 and reference electrode 26 being arranged in respectively in the substrate 21, the first insulating barrier, the second insulating barrier of hierarchic structure, each electrode is connected to gold PAD22 processed by golden wire 23 and goes up, and PAD22 is corresponding one by one with each electrode.
Embodiment 3
Ring-type microelectrode
The ring-type microelectrode of the glucose sensor of micro-meter scale shown in Fig. 5 microelectrode, the front portion of substrate 27 is column type, diameter 0.1-1mm, microelectrode total length is 0.5-8cm, the area of each electrode is 0.1-10mm
2, to electrode 30, working electrode 31 and reference electrode 32 annular distribution, in substrate 27, each electrode is upper by be connected to gold PAD28 processed through the golden wire 29 of dielectric base inside, and PAD28 is corresponding one by one with each electrode.
Embodiment 4
Helical form microelectrode
The helical form microelectrode of the glucose sensor of micro-meter scale shown in Fig. 6 microelectrode, the front portion of substrate 33 is column type, diameter>=0.1 and < 1mm, and bung flange internal diameter is 0.2-1mm, microelectrode total length is 0.5-8cm, and the area of each electrode is 0.1-10mm
2to electrode 36 and working electrode 37 annular distribution, in substrate 33, reference electrode 38 is wrapped in around base material 33 with helical form form, and reference electrode 38 not with other electrode contacts, it is upper that each electrode is connected to gold PAD34 processed by golden wire 35, and PAD34 is corresponding one by one with each electrode.
Embodiment 5
By carbon nanotube dispersed in Nafion solution
The present embodiment adopts multi-walled carbon nano-tubes, first with strong acid, CNT is carried out to acidify completely and oxidation, and then with the effect of Nafion aqueous solution, obtain CNT-Nafion dispersion liquid, be coated to again on electrode surface, step is: get MWCNTs and be scattered in 4.0mol/L hydrochloric acid, supersound process is after 4 hours, is washed to neutrality with second distillation; Use again chloroazotic acid supersound process 4 hours, wash with water to neutrality, 100 degrees Celsius of vacuum dryings 4 hours; Take the MWCNTs of above-mentioned processing, be dissolved in the mixed solution of 5%Nafion and 0.05mol/L phosphate buffer (pH7.0), ultrasonic dispersion 30 minutes, gets final product to obtain finely disseminated Nafion-MWCNTs black suspension.Above-mentioned suspension is coated on electrode surface, can be by modes such as brushing, spin coating, dip-coating, sprayings.Finally can obtain having carbon nano tube modified sensor electrode.The advantage of the method is that step is simple, and production cost is lower.
Embodiment 6
Covalency fixed carbon nanotube
The present embodiment adopts multi-walled carbon nano-tubes, first self assembly one deck sulfydryl second ammonia on metal electrode, remove impurity, multi-walled carbon nano-tubes solution after carboxylated are dropped on electrode surface and can obtain carbon nano tube modified electrode, step is: get multi-walled carbon nano-tubes and under 350 degrees Celsius, be oxidized 2 hours, remove metal oxide catalyst, after cooling in concentrated hydrochloric acid ultrasonic 4 hours, then washing and drying; CNT good purification is placed in to chloroazotic acid ultrasonic 6 hours again, by the CNT centrifugal filtration of gained, with deionized water wash to pH value be till 7 o'clock; By electrode process ultrasonic cleaning and H
2sO
4in electrochemical cleaning after, the alcoholic solution of putting into 1mmol/L mercaptoethylmaine soaks 2 hours, takes out and cleans, and at electrode surface, forms self assembly mercaptoethylmaine film; Then electrode is put into carbon nano-tube solution and soak 5 hours, then wash with water, dry and obtain carbon nano tube modified sensor electrode.The advantage of the method is that CNT is combined by covalent effect with electrode, and more firm, stability is stronger.
Embodiment 7
Poly-D-lysine is fixed carbon nanotube and enzyme simultaneously
The present embodiment adds CNT and enzyme (preference is glucoseoxidase) in poly-D-lysine (Polylysine) solution, after ultrasonic dispersion, be coated on electrode surface, step is: according to the step of embodiment 6, carry out the carboxylated of MWCNTs, then get carboxylated MWCNTs second distillation aqueous dispersion, then to adding in carbon nano-tube solution EDC (100mg/mL) and NHS (100mg/mL) NaOH to adjust pH value of solution, be 6.0, be placed under room temperature and stir 2 hours, and then this pH is adjusted into 8.5, add 200uL poly-D-lysine in above-mentioned carbon nano-tube solution with microsyringe, stirring reaction spends the night and obtains CNT-lysine solution, this solution of centrifugalize, remove the poly-D-lysine of not participating in reaction, then with intermediate water, disperse CNT-lysine, add glutaraldehyde water solution (25%), priming reaction 1～2 hour, add again glucoseoxidase (Gox), cross-linking reaction 2～3 hours, the glucoseoxidase of not participating in reaction is removed in centrifugalize, the most handy phosphate buffer (pH 7.4) disperses precipitation, obtain CNT-lysine-Gox solution, above-mentioned mixed solution is coated on platinum electrode, then under 4 degrees Celsius, place approximately 4 hours, after treating solvent evaporate to dryness, make carbon nano tube modified enzyme sensor electrode.The feature of the method is CNT and enzyme to be fixed on electrode surface simultaneously simultaneously.CNT, except improving electrode performance, has also played the effect of zymophore.
Embodiment 8
Electroreduction method modified nano gold
The present embodiment adopt electroreduction gold chloride legal system standby decorated by nano-gold electrode, step is: metal electrode is carried out, after the cleaning treatment such as ultrasonic, being placed in the chlorauric acid solution of 2mg/ml, with the KNO of 0.1mol/L
3solution does supporting electrolyte, reduces HAuCl under-200mV current potential
4solution, takes out and cleans after certain hour, can obtain the electrode of decorated by nano-gold.
Embodiment 9
Sol-gel process modified nano gold
The present embodiment adopts Na
2siO
3.9H
2o is placed in baking oven, places approximately 12 hours under 120 degrees Celsius, obtains Na
2siO
3.3H
2o, the completely cooling rear hydrochloric acid solution with 3mol/L regulates proportion to 1.38, obtains the water glass solution of clarification after filtration, takes out 1ml water 1:1 (V/V) dilution, then after sulfonic acid fundamental mode cation exchange column, obtains the Ludox of pH=1.5, standby; Metal electrode is carried out after pretreatment, immersing the 0.3ml above-mentioned Ludox making, 0.15mL cysteine (10mM), 0.20mLPVA(mass fraction is 0.05%), in the even mixed liquor of 10mL aurosol, within approximately 30 seconds, take out afterwards, under 4 degrees Celsius, place the electrode that obtains decorated by nano-gold after 12 hours.With respect to conventional electrodes, adopt said method to there is high effective area with the electrode after decorated by nano-gold, there is more binding site with enzyme etc. simultaneously, can more effectively carry out the detection of testing molecule.
Embodiment 10
The response contrast to hydrogen peroxide of two plane type microelectrode and common one side microelectrode
(thickness is about 0.01mm in politef (Teflon) substrate of the present embodiment employing same size, width is about 0.15mm), make one side microelectrode and double-sided microelectrodes, double-face electrode structure (is positioned in a plane electrode and reference electrode as shown in Figure 2, working electrode is positioned in another plane), single-side electrode structure is as shown in Figure 3.Working electrode and be platinum to electrode, reference electrode is silver/silver chloride, wherein, double-face electrode working electrode, to electrode, the area of reference electrode is respectively 0.9 square millimeter, 1.5 square millimeter, 0.4 square millimeter, wherein, single-side electrode working electrode, to electrode, the area of reference electrode is respectively 0.36 square millimeter, 0.6 square millimeter, 0.2 square millimeter, in 1mM hydrogenperoxide steam generator, single-side electrode is 1850nA to the response current of hydrogen peroxide, double-face electrode is 4120nA to hydrogen peroxide response current, this result absolutely proves that two plane type microelectrode makes full use of the working region upper and lower surface of substrate, the effective area of working electrode is 2.5 times of single-side electrode working electrode, to the hydrogen peroxide response current of same concentration, be single-side electrode 2.3 times.
The response contrast to hydrogen peroxide of helical form microelectrode and double-sided microelectrodes
The present embodiment adopts the bar-shaped substrate of diameter 0.15mm, make helical form microelectrode, adopting width is that 0.15mm sheet form base is prepared double-sided microelectrodes, double-face electrode structure (is positioned in a plane electrode and reference electrode as shown in Figure 2, working electrode is positioned in another plane), spiral electrode structure is as shown in Figure 6.Working electrode and be platinum to electrode, reference electrode is silver/silver chloride, wherein, double-face electrode working electrode, to electrode, the area of reference electrode is respectively 0.9 square millimeter, 1.5 square millimeter, 0.4 square millimeter, wherein, spiral electrode working electrode, to electrode, the area of reference electrode is respectively 1.36 square millimeters, 2.1 square millimeter, 0.75 square millimeter, reference electrode is thread, and around the working region of substrate, (spiral internal diameter is as 0.2mm take spiral form uniform winding, remaining electrode with annular distribution on working region, the position of reference electrode is corresponding with the position of remaining electrode, and reference electrode does not contact with remaining electrode), in 1mM hydrogenperoxide steam generator, double-face electrode is 4120nA to hydrogen peroxide response current, helical form microelectrode is 5480nA to the response current of hydrogen peroxide, this result absolutely proves that the used region that helical form microelectrode makes full use of substrate prepares electrode, the effective area of working electrode is 1.5 times of double-face electrode working electrode, to the hydrogen peroxide response current of same concentration, be single-side electrode 1.33 times, effectively improved the response intensity of electric current.
In sum, this utility model has effectively overcome various shortcoming of the prior art and tool high industrial utilization.
Above-described embodiment is illustrative principle of the present utility model and effect thereof only, but not for limiting this utility model.Any person skilled in the art scholar all can, under spirit of the present utility model and category, modify or change above-described embodiment.Therefore, have in technical field under such as and conventionally know that the knowledgeable modifies or changes not departing from all equivalences that complete under spirit that this utility model discloses and technological thought, must be contained by claim of the present utility model.
Claims (9)
1. a micro-meter scale glucose sensor microelectrode, comprise substrate, working electrode, to electrode, it is characterized in that, the front portion of described substrate is working region, described working electrode, electrode is positioned on the working region of described substrate, maximum gauge≤1 millimeter of the cross section of described working region, the afterbody of described substrate is provided with and each electrode PAD one to one, described working electrode, electrode is all connected by the wire PAD corresponding with it.
2. a kind of micro-meter scale glucose sensor microelectrode as claimed in claim 1, it is characterized in that, described micro-meter scale glucose sensor microelectrode is two plane type structure, the working region of its substrate is plate, also comprise reference electrode, described electrode and reference electrode are positioned in a plane of working region of substrate, working electrode is positioned in another plane of working region of substrate.
3. a kind of micro-meter scale glucose sensor microelectrode as claimed in claim 1, it is characterized in that, described micro-meter scale glucose sensor microelectrode is notch cuttype multiple structure, also comprise reference electrode, the first insulating barrier and the second insulating barrier, described the first insulating barrier is positioned in described substrate, described the first insulating barrier be shorter in length than substrate, described the second insulating barrier is positioned on the first insulating barrier, described the second insulating barrier be shorter in length than the first insulating barrier, described the first insulating barrier, the second insulating barrier, the afterbody of substrate overlaps, described the first insulating barrier, the second insulating barrier, substrate forms the three-decker of notch cuttype, described working electrode, reference electrode and electrode being laid respectively in the Different Plane of three-decker of notch cuttype.
4. a kind of micro-meter scale glucose sensor microelectrode as claimed in claim 1, it is characterized in that, described micro-meter scale glucose sensor microelectrode is circulus, the working region of its substrate is column type or taper, also comprise reference electrode, each electrode all with annular distribution on working region.
5. a kind of micro-meter scale glucose sensor microelectrode as claimed in claim 1, it is characterized in that, described micro-meter scale glucose sensor microelectrode is helical structure, the working region of its substrate is column type or taper, also comprise reference electrode, described working electrode, to any one in electrode and reference electrode, be thread, and with spiral form uniform winding around the working region of substrate, all the other two electrodes with annular distribution on working region.
6. a kind of micro-meter scale glucose sensor microelectrode as claimed in claim 1, it is characterized in that, described micro-meter scale glucose sensor microelectrode is helical structure, the working region of its substrate is column type or taper, also comprise reference electrode and auxiliary electrode, described working electrode, to any one in electrode, auxiliary electrode and reference electrode, be thread, and with spiral form uniform winding around the working region of substrate, its excess-three electrode with annular distribution on working region.
7. a kind of micro-meter scale glucose sensor microelectrode as claimed in claim 1, is characterized in that, described electrode is selected to the one in graphite electrode, glass-carbon electrode, noble metal electrode etc.
8. a kind of micro-meter scale glucose sensor microelectrode as claimed in claim 1, is characterized in that, described working electrode is selected from the one in graphite electrode, glass-carbon electrode, noble metal electrode etc.
9. a kind of micro-meter scale glucose sensor microelectrode as described in claim as arbitrary in claim 2-6, is characterized in that, described reference electrode is selected from the one in silver chloride/silver electrode or calomel electrode.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103462615A (en) * | 2013-09-13 | 2013-12-25 | 上海移宇科技有限公司 | Micrometer-scale glucose sensor microelectrode |
| CN105388199A (en) * | 2015-12-25 | 2016-03-09 | 张萍 | Electrode blood glucose meter with telecommunication function and high antijamming capability |
| CN111870238A (en) * | 2019-05-03 | 2020-11-03 | 乌宁 | Implanted biosensor and manufacturing method thereof |
-
2013
- 2013-09-13 CN CN201320570566.9U patent/CN203555741U/en not_active Withdrawn - After Issue
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103462615A (en) * | 2013-09-13 | 2013-12-25 | 上海移宇科技有限公司 | Micrometer-scale glucose sensor microelectrode |
| CN103462615B (en) * | 2013-09-13 | 2015-12-16 | 上海移宇科技有限公司 | Micrometer-scale glucose sensor microelectrode |
| CN105388199A (en) * | 2015-12-25 | 2016-03-09 | 张萍 | Electrode blood glucose meter with telecommunication function and high antijamming capability |
| CN111870238A (en) * | 2019-05-03 | 2020-11-03 | 乌宁 | Implanted biosensor and manufacturing method thereof |
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