CN104749228A - Test strip and method for detecting blood glucose and blood lipid simultaneously - Google Patents
Test strip and method for detecting blood glucose and blood lipid simultaneously Download PDFInfo
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- CN104749228A CN104749228A CN201310749689.3A CN201310749689A CN104749228A CN 104749228 A CN104749228 A CN 104749228A CN 201310749689 A CN201310749689 A CN 201310749689A CN 104749228 A CN104749228 A CN 104749228A
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Abstract
The invention discloses a test strip and a method for detecting blood glucose and blood lipid simultaneously, belonging to the field of the biochemical index detection technology. The test strip comprises a base plate layer, at least two reaction pads and a diffusion layer, wherein enzymes for detecting blood glucose and blood lipid are arranged on the reaction pads respectively, and the at least two reaction pads are both communicated with the diffusion layer; and detection electrode pairs are arranged on the base plate layer, the number of the detection electrode pairs is consistent with that of the reaction pads, and each detection electrode corresponds to one reaction pad. The method for detecting blood glucose and blood lipid simultaneously adopts the test strip, a liquid sample is uniformly diffused to all the reaction pads through the diffusion layer, and the contents of blood glucose and blood lipid in the liquid sample can be acquired respectively. According to the test strip and the method for detecting blood glucose and blood lipid simultaneously, blood glucose and blood lipid can be detected simultaneously through once sample injection, the volume of the liquid sample is reduced, the detection time is shorted and the operating complexity degree is reduced.
Description
Technical field
The present invention relates to a kind of biochemical indicator detection technique, particularly relate to a kind of test strips and the method that detect blood sugar and blood fat simultaneously.
Background technology
In biochemical indicator detects, electrochemical assay method is the electron transmission produced after enzyme carries out biochemical reaction, according to the current flow that test substance concentration in a corpse or other object for laboratory examination and chemical testing is directly proportional, detect through conductive electrode again, the characteristic that its rapid reaction and electronics are passed to electrode fast and measure, makes electrochemical determination method become rapid and reliable detection method.Electric pole type test film due to its easily manufacture, with low cost and be easy to carryly applied to various testing product at large, the various biochemistry detection devices such as such as blood sugar, uric acid, cholesterol.
As everyone knows, diabetic belongs to the people at highest risk of angiocardiopathy, according to investigations, has nearly eighty per cant diabetic finally to die from angiocardiopathy.And in order to defend cardiovascular health, diabetic needs to have constructed three roads " fire wall "---blood sugar, blood pressure, blood fat.Blood sugar is more easily subject to the attention of diabetic naturally, and blood pressure is due to measurement means economy convenience, and also than being easier to carry out monitor and forecast, comparatively speaking, the control of blood fat is more prone to out in the cold.But dyslipidemia is wilfully the direct saboteur of cardiovascular health, and therefore, numerous diabetics must be paid attention to.So for diabetes patient, except to except the monitoring of blood sugar, the monitoring of blood fat is also vital.
But, so far, can detect the index of blood sugar and blood fat without any test strips simultaneously, the blood sugar of diabetic and blood fat can be made to be well controlled.
Summary of the invention
Based on this, the object of the invention is to the defect overcoming prior art, a kind of test strips simultaneously detecting blood sugar and blood fat is provided, adopts this test strips, in a test strips, blood sugar and blood fat can be detected by single injected sampling simultaneously, realize integration and detect.
For achieving the above object, the present invention takes following technical scheme:
Detect a test strips for blood sugar and blood fat simultaneously, comprise substrate layer, reacting pad and diffusion layer; Described reacting pad is at least two, it is respectively equipped with the enzyme for detecting blood sugar and the enzyme for detecting blood fat; Described at least two reacting pads are all communicated with described diffusion layer; Described substrate layer is provided with detecting electrode pair, and the right quantity of this detecting electrode is consistent with the quantity of reacting pad, and each detecting electrode is to all corresponding with a reacting pad.
Test strips of the present invention, has multiple reacting pad, and the quantity of this reacting pad can be arranged flexibly according to the biochemical indicator that will detect, and adopts multiple detecting electrode pair, make each reacting pad all with a pair detecting electrode to corresponding, the detection of disparity items can be realized.And by the setting of diffusion layer, liquor sample is enable to diffuse to the reacting pad communicated with diffusion layer uniformly, ensure that each reacting pad all can obtain the liquor sample being enough to carry out detecting, reach once take a blood sample, application of sample can detect the object of blood sugar and blood fat simultaneously.
Wherein in an embodiment, also comprise hydrophilic film and bonding coat, described bonding coat is provided with hollow-out parts, and this hollow-out parts is provided with the siphon passage being communicated with test strips edge; Described hydrophilic film covers described hollow-out parts.Due to the existence of hollow-out parts, form the application of sample space that space size is fixing between described hydrophilic film and diffusion layer, by the restriction in this application of sample space, ensure that each application of sample amount is all the same, ensure that high repeatability and the precision of test strips.
Wherein in an embodiment, described detecting electrode is to comprising the relative working electrode in position and to electrode, described substrate layer comprises first substrate layer and second substrate layer, this first substrate layer is provided with the electrode tips joint of working electrode, working electrode wire and working electrode, and this second substrate layer is provided with to electrode, to electrode cable and the electrode tips joint to electrode.Described detecting electrode is to being carbon electrode, graphite electrode or metal electrode etc.; Described wire is silver-colored wire or golden wire etc.By working electrode be located on different substrate layers respectively to electrode, when detecting, electric current need flow through whole reacting pad, can provide larger test volume.
Wherein in an embodiment, also comprise insulation course, described insulation course is located between first substrate layer and second substrate layer, and this insulation course is provided with the first perforate with reacting pad form fit.By the use of insulation course, can better completely cut off working electrode and to electrode, guarantee the accuracy detected, and by institute's apertured on insulation course, reacting pad is exposed, ensure reacting pad and working electrode with to being communicated with between electrode.
Wherein in an embodiment, also comprise separating pad and flow deflector, described separating pad is located between diffusion layer and reacting pad, and described flow deflector is located between separating pad and reacting pad.By the setting of separating pad, interfering material can be separated, avoid it to enter in reacting pad, this separating pad can adopt the materials such as glass fiber filter paper to make.And utilize flow deflector, ensure that liquor sample can be entered in reacting pad by separating pad smoothly.
Wherein in an embodiment, the described enzyme for detecting blood fat is at least one in cholesterol esterase, cholesterol oxidase, peroxidase, hydrogen peroxidase, lipoproteinesterase, glycerokinase, GPO, and the described enzyme for detecting blood sugar is at least one in hydrogen peroxidase, peroxidase, glucose oxidase.Adopt the above-mentioned enzyme for detecting blood fat to detect TC(T-CHOL in blood fat), HDL(high-density lipoprotein (HDL)), TG(triglyceride), LDL(low-density lipoprotein), VLDL(very low density lipoprotein (VLDL)) in any one or a few composition, wherein, the enzyme detecting TC, HDL, LDL, VLDL is cholesterol esterase, cholesterol oxidase, at least one in peroxidase; The enzyme of detection TG is at least one in lipoproteinesterase, glycerokinase, GPO, peroxidase.According to different demands, choose required enzyme flexibly.
Wherein in an embodiment, described reacting pad is also provided with electron transfer mediator and damping fluid; Described electron transfer mediator is ferricyanate (such as the potassium ferricyanide etc.), ferrocene (i.e. cyclopentadienyl group iron) or 1,4-benzoquinone; Described damping fluid is phosphate buffer, three (methylol) aminomethane-hydrochloride buffer (TRIS damping fluid) or 2-(N-morpholine) ethanesulfonic acid buffer (MES damping fluid).Meanwhile, also as required, can add some other materials, such as Triton X-100 (Triton X-100) etc., in order to improve the solubleness of ester class cholesterol.In order to reach better Detection results, according to different situations, various electron transfer mediator can be chosen in order to reduce the electromotive force needed for redox reaction, or choosing surfactant in order to increase the solubleness of measured object.
Wherein in an embodiment, described substrate layer is polystyrene, polyester, polycarbonate, polyethers, tygon, polypropylene, polyethylene terephthalate or PET substrate's layer.Substrate layer is made with insulating polymer material, guarantees that the right work of detecting electrode is interference-free.
The present invention also provides a kind of method simultaneously detecting blood sugar and blood fat, adopt above-mentioned test strips, liquor sample is added on diffusion layer, by diffusion layer, liquor sample is diffused to each reacting pad uniformly, liquor sample on different reacting pads respectively with detect the enzyme of blood sugar and detect the enzyme reaction of blood fat, generation current, according to size of current, draws the content of blood sugar and blood fat in liquor sample respectively.
The method simultaneously detecting blood sugar and blood fat of the present invention, and by the setting of diffusion layer, liquor sample is enable to diffuse to the multiple reacting pads communicated with diffusion layer uniformly, ensure that each reacting pad all can obtain the liquor sample being enough to carry out detecting, and the enzyme arranged on multiple reacting pad needed for different test item, with the object reaching once blood sampling, application of sample can detect blood sugar and blood fat simultaneously.
Wherein in an embodiment, this test strips also comprises hydrophilic film and bonding coat, and described bonding coat is provided with hollow-out parts, and this hollow-out parts is provided with the siphon passage being communicated with test strips edge; Described hydrophilic film covers described hollow-out parts; Utilize the guiding of hydrophilic film, in the mode of siphon, liquor sample is sucked into hollow-out parts by siphon passage, with the application of sample amount of the space size confined liquid sample of hollow-out parts, then make liquor sample diffuse to each reacting pad uniformly by diffusion layer, carry out subsequent reactions.With the mode sample introduction of siphon, once after the siphon passage at test strips edge touches liquor sample, liquor sample just can enter reaction zone automatically, reduces the difficulty of test of user, and makes liquor sample amount reduce to some extent.Meanwhile, also due to the existence of hollow-out parts, form the application of sample space that space size is fixing between described hydrophilic film and diffusion layer, by the restriction in this application of sample space, ensure that each application of sample amount is all the same, ensure that high repeatability and the precision of test strips.
Compared with prior art, the present invention has following beneficial effect:
A kind of test strips simultaneously detecting blood sugar and blood fat of the present invention, has multiple reacting pad, can detect blood sugar and blood fat simultaneously, realizes integration and detects.Detect blood sugar and blood fat by single injected sampling simultaneously, decrease the volume of liquor sample, shorten detection time, reduce the fussy degree of operation.Avoid the trouble changed test strips and bring, diabetic can be impelled to identify dyslipidemia early and give correct intervention, atherosclerotic can be prevented and treated, reduce cardiocerebrovasculaevents events, reduce mortality ratio.
Further, this test strips, also by the restriction in application of sample space, ensure that each application of sample amount is all the same, ensure that high repeatability and the precision of test strips.
A kind of method simultaneously detecting blood sugar and blood fat of the present invention, can detect blood sugar and blood fat fast by single injected sampling simultaneously, reduce the fussy degree of operation.
Accompanying drawing explanation
Fig. 1 is the test strips schematic diagram in embodiment 1;
Fig. 2 is the test strips schematic diagram in embodiment 2.
Embodiment
The present invention is described in detail below in conjunction with the drawings and specific embodiments.
Embodiment 1
A kind of test strips 100 simultaneously detecting blood sugar and blood fat, this embodiment adopts formula sample introduction of bleeding.This test strips 100 comprises first substrate layer 110, insulation course 120, four reacting pads (131,132,133 of closed assembly successively, 134), second substrate layer 140, flow deflector (151,152,153,154), separating pad 160, diffusion layer 170 and cover plate 180, as shown in Figure 1.Wherein:
In the present embodiment, use polyethylene terephthalate (PET) flat substrates as first substrate layer 110 and second substrate layer 140, the mode of recycling screen painting forms working electrode (111 on first substrate layer 110, 112, 113, 114), working electrode wire, the electrode tips joint (115 of working electrode, 116, 117, 118), this electrode tips joint is used for and executes alive device and be electrically connected, shape paired electrode (141 on second substrate layer 140, 142, 143, 144), to electrode cable, to the electrode tips joint 149 of electrode, and the second perforate (145 is being set on the position of electrode just to four working electrodes, 146, 147, 148) sample passes through for liquid, this working electrode be carbon electrode to electrode, wire is silver-colored wire.
Insulation course 120 is between first substrate layer 110 and second substrate layer 140, and insulation course 120 is provided with and reacting pad (131,132,133,134) first perforate (121,122 of form fit, 123,124), this insulation course 120 also exposes the right electrode tips joint of detecting electrode, for execute alive electronic installation and be electrically connected.
Reaction reagent contained by reacting pad (131,132,133,134) comprises electron transfer mediator, reacts with liquor sample and the enzyme of generation current and damping fluid.Wherein, electron transfer mediator is ferrocene.The enzyme reacting also generation current with liquor sample is cholesterol esterase, cholesterol oxidase, hydrogen peroxidase, and glucose oxidase, is located at respectively on four reacting pads (131,132,133,134).Damping fluid is TRIS damping fluid.In this embodiment, some other materials can also be added, such as Triton X-100 etc., in order to improve the solubleness of ester class cholesterol.
Flow deflector (151,152,153,154) the second perforate (145,146 that second substrate layer 140 is arranged is arranged on, 147,148) top, just in time overlaps with this second perforate, then covers on second substrate layer 140 successively by separating pad 160 and diffusion layer 170, then add upper cover plate 180, this cover plate 180 is provided with sample inlets 181.Bonded by bonding agent between the different layers of this test strips.
The test strips workflow of the present embodiment is as follows:
First, liquor sample drops to the sample inlets 181 on cover plate 180 in the mode dripped, through the even diffusion of diffusion layer 170, spread by diffusion layer 170 mediad surrounding, and respectively by flow deflector (151, 152, 153, 154), make liquor sample smoothly by the separating pad 160 under diffusion layer 170, after being separated removing chaff interference by separating pad 160, liquor sample is respectively by electrode (141, 142, 143, 144) upper the second perforate (145 arranged, 146, 147, 148) reacting pad (131 be positioned under second substrate layer 140 is entered, 132, 133, 134), at reacting pad (131, 132, 133, 134) in, determinand respectively with cholesterol esterase, cholesterol oxidase, hydrogen peroxidase or glucose oxidase enzyme reaction, generation current, now instrument starts capture program, but do not point out user to start to detect, until the signal intensity gathered is greater than predetermined threshold, represent that blood volume is just sufficient, now instrument display also reminding user has started to measure, user stops dripping blood.Finally according to the size of current that enzyme reaction in determinand in liquor sample and differential responses pad (131,132,133,134) produces, draw the content of blood sugar and blood fat in liquor sample respectively.
Embodiment 2
As shown in Figure 2, detect blood sugar while the present embodiment substantially identical with the test strips of embodiment 1 with the test strips 200 of blood fat, difference is:
This test strips also closed assembly bonding coat 290 and hydrophilic film 291 successively between diffusion layer and cover plate.This bonding coat 290 is made up of the sticking material of tool, hydrophilic film 291 can be adhered to.This hydrophilic film 291 is made up of the material with hydrophilic radical, liquor sample can be sucked.
Described bonding coat is provided with hollow-out parts 293, and this hollow-out parts 292 is provided with the siphon passage 293 being communicated with test strips edge; Described hydrophilic film 291 covers described hollow-out parts 292.
The test strips work of the present embodiment is substantially identical with the test strips workflow of embodiment 1, and difference is:
This test strips adopts the mode of siphon sample introduction, utilize the guiding of hydrophilic film 291, liquor sample is sucked into hollow-out parts 292 by siphon passage 293, with the application of sample amount of the space size confined liquid sample of hollow-out parts 292, liquor sample is made to diffuse to each reacting pad (231,232,233 uniformly by diffusion layer 270 again, 234), subsequent reactions is carried out.
The above embodiment only have expressed several embodiment of the present invention, and it describes comparatively concrete and detailed, but therefore can not be interpreted as the restriction to the scope of the claims of the present invention.It should be pointed out that for the person of ordinary skill of the art, without departing from the inventive concept of the premise, can also make some distortion and improvement, these all belong to protection scope of the present invention.Therefore, the protection domain of patent of the present invention should be as the criterion with claims.
Claims (10)
1. detect a test strips for blood sugar and blood fat simultaneously, it is characterized in that, comprise substrate layer, reacting pad and diffusion layer; Described reacting pad is at least two, it is respectively equipped with the enzyme for detecting blood sugar and the enzyme for detecting blood fat; Described at least two reacting pads are all communicated with described diffusion layer; Described substrate layer is provided with detecting electrode pair, and the right quantity of this detecting electrode is consistent with the quantity of reacting pad, and each detecting electrode is to all corresponding with a reacting pad.
2. the test strips simultaneously detecting blood sugar and blood fat according to claim 1, it is characterized in that, also comprise hydrophilic film and bonding coat, described bonding coat is provided with hollow-out parts, and this hollow-out parts is provided with the siphon passage being communicated with test strips edge; Described hydrophilic film covers described hollow-out parts.
3. the test strips simultaneously detecting blood sugar and blood fat according to claim 1, it is characterized in that, described detecting electrode is to comprising the relative working electrode in position and to electrode, described substrate layer comprises first substrate layer and second substrate layer, this first substrate layer is provided with the electrode tips joint of working electrode, working electrode wire and working electrode, and this second substrate layer is provided with to electrode, to electrode cable and the electrode tips joint to electrode.
4. the test strips simultaneously detecting blood sugar and blood fat according to claim 3, it is characterized in that, also comprise insulation course, described insulation course is located between first substrate layer and second substrate layer, and this insulation course is provided with the first perforate with reacting pad form fit.
5. the test strips simultaneously detecting blood sugar and blood fat according to claim 1, it is characterized in that, also comprise separating pad and flow deflector, described separating pad is located between diffusion layer and reacting pad, and described flow deflector is located between separating pad and reacting pad.
6. the test strips simultaneously detecting blood sugar and blood fat according to claim 1, it is characterized in that, the described enzyme for detecting blood fat is at least one in cholesterol esterase, cholesterol oxidase, peroxidase, hydrogen peroxidase, lipoproteinesterase, glycerokinase, GPO, and the described enzyme for detecting blood sugar is at least one in hydrogen peroxidase, peroxidase, glucose oxidase.
7. the test strips simultaneously detecting blood sugar and blood fat according to claim 1, is characterized in that, described reacting pad is also provided with electron transfer mediator and damping fluid; Described electron transfer mediator is ferricyanate, ferrocene or 1,4-benzoquinone; Described damping fluid is phosphate buffer, three (methylol) aminomethane-hydrochloride buffer or 2-(N-morpholine) ethanesulfonic acid buffer.
8. the test strips simultaneously detecting blood sugar and blood fat according to claim 1, it is characterized in that, described substrate layer is polystyrene, polyester, polycarbonate, polyethers, tygon, polypropylene, polyethylene terephthalate or PET substrate's layer.
9. one kind is detected the method for blood sugar and blood fat simultaneously, it is characterized in that: adopt test strips according to claim 1, liquor sample is added on diffusion layer, by diffusion layer, liquor sample is diffused to each reacting pad uniformly, liquor sample on different reacting pads respectively with detect the enzyme of blood sugar and detect the enzyme reaction of blood fat, generation current, according to size of current, draws the content of blood sugar and blood fat in liquor sample respectively.
10. the method simultaneously detecting blood sugar and blood fat according to claim 9, it is characterized in that, this test strips also comprises hydrophilic film and bonding coat, and described bonding coat is provided with hollow-out parts, and this hollow-out parts is provided with the siphon passage being communicated with test strips edge; Described hydrophilic film covers described hollow-out parts; Utilize the guiding of hydrophilic film, in the mode of siphon, liquor sample is sucked into hollow-out parts by siphon passage, with the application of sample amount of the space size confined liquid sample of hollow-out parts, then make liquor sample diffuse to each reacting pad uniformly by diffusion layer, carry out subsequent reactions.
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106680347A (en) * | 2017-02-16 | 2017-05-17 | 常州鹤田生物技术有限公司 | Finger blood test strip and use method and preparation method thereof |
| CN108398472A (en) * | 2018-04-29 | 2018-08-14 | 浙江大学山东工业技术研究院 | A kind of dual-port screen printing electrode having two-parameter detection function |
| CN108508074A (en) * | 2018-04-29 | 2018-09-07 | 浙江大学山东工业技术研究院 | A kind of anti-interference dual-port screen printing electrode |
| CN109916890A (en) * | 2019-04-12 | 2019-06-21 | 吉林省汇酉生物技术股份有限公司 | A kind of dry chemistry reagent piece and preparation method thereof quantitative determining total cholesterol concentration |
| CN109916894A (en) * | 2019-04-12 | 2019-06-21 | 吉林省汇酉生物技术股份有限公司 | A kind of dry chemistry reagent piece and preparation method thereof quantitative determining concentration of glucose |
| CN110568045A (en) * | 2019-09-12 | 2019-12-13 | 浙江大学山东工业技术研究院 | Electrochemical biosensor capable of rapidly detecting |
| CN113109405A (en) * | 2021-04-01 | 2021-07-13 | 广州南雪医疗器械有限公司 | Test paper for detecting triglyceride |
| CN113219168A (en) * | 2021-05-06 | 2021-08-06 | 黄波 | Colloidal gold test strip |
| WO2022036851A1 (en) * | 2020-08-18 | 2022-02-24 | 美康生物科技股份有限公司 | Blood lipid test card and use thereof |
| CN114798020A (en) * | 2022-03-28 | 2022-07-29 | 四川成电医联科技咨询有限公司 | Multi-channel electrochemical micro-fluidic test card and manufacturing method thereof |
| WO2022252744A1 (en) * | 2019-08-19 | 2022-12-08 | Medtrum Technologies Inc. | Analyte detection device |
| CN115684611A (en) * | 2022-12-30 | 2023-02-03 | 天津德祥生物技术股份有限公司 | Erythrocyte antigen detection test strip and application |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106680347A (en) * | 2017-02-16 | 2017-05-17 | 常州鹤田生物技术有限公司 | Finger blood test strip and use method and preparation method thereof |
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| WO2022036851A1 (en) * | 2020-08-18 | 2022-02-24 | 美康生物科技股份有限公司 | Blood lipid test card and use thereof |
| CN113109405A (en) * | 2021-04-01 | 2021-07-13 | 广州南雪医疗器械有限公司 | Test paper for detecting triglyceride |
| CN113219168A (en) * | 2021-05-06 | 2021-08-06 | 黄波 | Colloidal gold test strip |
| CN114798020A (en) * | 2022-03-28 | 2022-07-29 | 四川成电医联科技咨询有限公司 | Multi-channel electrochemical micro-fluidic test card and manufacturing method thereof |
| CN115684611A (en) * | 2022-12-30 | 2023-02-03 | 天津德祥生物技术股份有限公司 | Erythrocyte antigen detection test strip and application |
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