CN104749228B - The test strips and method of blood glucose and blood fat are detected simultaneously - Google Patents
The test strips and method of blood glucose and blood fat are detected simultaneously Download PDFInfo
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- CN104749228B CN104749228B CN201310749689.3A CN201310749689A CN104749228B CN 104749228 B CN104749228 B CN 104749228B CN 201310749689 A CN201310749689 A CN 201310749689A CN 104749228 B CN104749228 B CN 104749228B
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Abstract
The invention discloses a kind of while detect the test strips and method of blood glucose and blood fat, belong to biochemical indicator detection technique field.The test strips include substrate layer, reacting pad and diffusion layer；Reacting pad at least two is respectively provided with the enzyme of detection blood glucose and the enzyme for detecting blood fat thereon；At least two reacting pad is connected with the diffusion layer；Substrate layer is equipped with detecting electrode pair, and the quantity of the detecting electrode pair is consistent with the quantity of reacting pad, and each detecting electrode is to corresponding with a reacting pad.The method of blood glucose and blood fat is detected while of the invention using above-mentioned test strips, liquor sample is uniformly diffused to each reacting pad by diffusion layer, so as to obtain the content of blood glucose and blood fat in liquor sample respectively.The test strips and method of the present invention, detect blood glucose and blood fat by single injected sampling, reduce the volume of liquor sample, shorten detection time, reduce the fussy degree of operation simultaneously.
Description
Technical field
The present invention relates to a kind of biochemical indicator detection technique, more particularly to a kind of while detect the test paper of blood glucose and blood fat
Item and method.
Background technology
In biochemical indicator detection, the assay method of electrochemistry is that the electronics that enzyme generated after biochemical reaction passes
It passs, is detected according to the directly proportional current flow of test substance concentration in a specimen, then through conductive electrode, fast reaction and electronics are quick
The characteristic for being transferred to electrode and measuring makes electrochemical determination method become rapid and reliable detection method.Electric pole type test film by
In its easy to manufacture, of low cost and easy to carry be generally applicable to various detection products, such as blood glucose, uric acid, courage are solid
The various biochemistry detection devices such as alcohol.
It is well known that diabetic belongs to the people at highest risk of angiocardiopathy, according to investigations, there is nearly eighty per cant diabetic
Ultimately succumb to angiocardiopathy.And in order to defend cardiovascular health, diabetic needs to have constructed three " fire walls " ---
Blood glucose, blood pressure, blood fat.Blood glucose is easier to be paid attention to by diabetic naturally, and blood pressure is since measurement means economy is convenient,
Also it is easier to be monitored and control, in contrast, the control of blood fat is more prone to ignored.However, dyslipidemia is wilfully
It is the direct saboteur of cardiovascular health, therefore, numerous diabetics must be paid attention to.So diabetes patient is come
It says, other than the monitoring to blood glucose, the monitoring of blood fat is also vital.
But so far, the index of blood glucose and blood fat can be detected simultaneously without any test strips, can made
The blood glucose and blood fat of diabetic is well controlled.
Invention content
Based on this, the defects of it is an object of the invention to overcome the prior art, provide a kind of while detect blood glucose and blood fat
Test strips, using the test strips, blood glucose and blood fat can be detected simultaneously by single injected sampling in a test strips, realize one
Bodyization detects.
To achieve the above object, the present invention takes following technical scheme：
Test strips that are a kind of while detecting blood glucose and blood fat, including substrate layer, reacting pad and diffusion layer；The reacting pad is extremely
It is less two, is respectively provided with the enzyme of detection blood glucose and the enzyme for detecting blood fat thereon；At least two reacting pad is equal
It is connected with the diffusion layer；The substrate layer is equipped with detecting electrode pair, the quantity of the detecting electrode pair and the quantity of reacting pad
Unanimously, and each detecting electrode is to corresponding with a reacting pad.
The test strips of the present invention have multiple reacting pads, and the quantity of the reacting pad can be according to the biochemical indicator to be detected spirit
Setting living, and multiple detecting electrodes pair are used, each reacting pad is made, to corresponding, can be realized not with a pair of of detecting electrode
With the detection of project.And pass through the setting of diffusion layer, liquor sample is enable uniformly to diffuse to and is reacted with what diffusion layer communicated
Pad ensures that each reacting pad can obtain the liquor sample for being sufficient for detection, reaches primary blood sampling, sample-adding can be examined simultaneously
Survey the purpose of blood glucose and blood fat.
Hydrophilic film and adhesive layer are further included in one of the embodiments, and the adhesive layer is equipped with hollow-out parts, the hollow out
Portion is equipped with the siphon passage at connection test strips edge；The hydrophilic film covers the hollow-out parts.It is described due to the presence of hollow-out parts
The fixed sample-adding space of space size is formed between hydrophilic film and diffusion layer, by the limitation in the sample-adding space, ensure that every time
Sample-adding amount is the same, it is ensured that the high repeatability and precision of test strips.
The detecting electrode is described to including the opposite working electrode in position and to electrode in one of the embodiments,
Substrate layer includes first substrate layer and second substrate layer, which is equipped with working electrode, working electrode conducting wire and work
Make the electrode tips connector of electrode, which is equipped with and is connect to electrode, to electrode cable and to the electrode tips of electrode
Head.The detecting electrode is to for carbon electrode, graphite electrode or metal electrode etc.；The conducting wire is silver wire or golden conducting wire etc..It will
Working electrode and electrode is respectively arranged on different substrate layers, when detecting, electric current need to flow through entire reacting pad, be capable of providing
The test volume of bigger.
Insulating layer is further included in one of the embodiments, and the insulating layer is set on first substrate layer and second substrate layer
Between, which is equipped with and matched first trepanning of reacting pad shape.By the use of insulating layer, can preferably completely cut off
Working electrode and to electrode, it is ensured that the accuracy of detection, and pass through institute's apertured on insulating layer, reacting pad is exposed, ensures reaction
Pad and working electrode and the connection between electrode.
Separating pad and flow deflector are further included in one of the embodiments, and the separating pad is set on diffusion layer and reacting pad
Between, the flow deflector is set between separating pad and reacting pad.By the setting of separating pad, interfering substance can be detached, kept away
Exempt from it to enter in reacting pad, which can be used the materials such as glass fiber filter paper and be made.And it using flow deflector, ensure that
Liquor sample can pass through separating pad and enter in reacting pad.
The enzyme for being used to detect blood fat is cholesterol esterase, cholesterol oxidase, peroxide in one of the embodiments,
At least one of compound enzyme, catalase, lipoproteinesterase, glycerokinase, phosphoglycerol oxidase, it is described to be used to detect
The enzyme of blood glucose is at least one of catalase, peroxidase, glucose oxidase.It is used to detect blood fat using above-mentioned
Enzyme detect TC in blood fat（T-CHOL）、HDL（High-density lipoprotein）、TG（Triglyceride）、LDL（Low-density lipoprotein
In vain）、VLDL（Very low density lipoprotein）In any one or a few composition, wherein, detection TC, HDL, LDL, VLDL enzyme be
At least one of cholesterol esterase, cholesterol oxidase, peroxidase；The enzyme of detection TG is lipoproteinesterase, glycerine swashs
At least one of enzyme, phosphoglycerol oxidase, peroxidase.According to different demands, required enzyme is flexibly chosen.
In one of the embodiments, electron transfer mediator and buffer solution are additionally provided on the reacting pad；The electronics passes
Medium is passed as ferricyanate（Such as the potassium ferricyanide etc.）, ferrocene（That is cyclopentadienyl group iron）Or 1,4-benzoquinone；The buffer solution is
Phosphate buffer, three (methylol) aminomethane-hydrochloride buffers（TRIS buffer solutions）Or 2- (N- morpholines) ethanesulfonic acid buffering
Liquid（MES buffer solutions）.Meanwhile also can be as needed, some other substances are added in, such as (polyethylene glycol is pungent by Triton X-100
Base phenyl ether) etc., to improve the solubility of esters cholesterol.It, can be according to not sympathizing in order to reach better detection result
Condition chooses various electron transfer mediators to reduce potential needed for redox reaction or choose surfactant increasing
Add the solubility of measured object.
The substrate layer is polystyrene, polyester, makrolon, polyethers, polyethylene, gathers in one of the embodiments,
Propylene, polyethylene terephthalate or PET substrate's layer.Substrate layer is with insulating polymer material system
Into, it is ensured that the work of detecting electrode pair is interference-free.
The present invention also provides method that is a kind of while detecting blood glucose and blood fat, using above-mentioned test strips, by liquor sample
It is added on diffusion layer, liquor sample is uniformly diffused to each reacting pad by diffusion layer, liquor sample is in different reacting pads
The upper enzyme reaction with the enzyme of detection blood glucose and detection blood fat respectively, generates electric current, according to size of current, obtains liquor sample respectively
The content of middle blood glucose and blood fat.
The method of blood glucose and blood fat is detected while of the invention, and passes through the setting of diffusion layer, enables liquor sample equal
Even diffuses to the multiple reacting pads communicated with diffusion layer, ensures that each reacting pad can obtain the liquid for being sufficient for detection
Sample, and the enzyme needed for different detection projects is set on multiple reacting pads, to reach primary blood sampling, sample-adding can detect simultaneously
The purpose of blood glucose and blood fat.
The test strips further include hydrophilic film and adhesive layer in one of the embodiments, and the adhesive layer is equipped with hollow out
Portion, the hollow-out parts are equipped with the siphon passage at connection test strips edge；The hydrophilic film covers the hollow-out parts；Utilize hydrophilic film
Guiding, in a manner of siphon, hollow-out parts are sucked by liquor sample by siphon passage, and liquid is limited with the space size of hollow-out parts
The sample-adding amount of sample, then liquor sample is made uniformly to diffuse to each reacting pad by diffusion layer, carry out subsequent reactions.With siphon
Mode sample introduction, after the siphon passage at test strips edge touches liquor sample, liquor sample can automatically into reaction zone,
The difficulty of test of user is reduced, and is reduced liquor sample amount.Meanwhile due also to the presence of hollow-out parts, described
The fixed sample-adding space of space size is formed between hydrophilic film and diffusion layer, by the limitation in the sample-adding space, ensure that every time
Sample-adding amount is the same, it is ensured that the high repeatability and precision of test strips.
Compared with prior art, the invention has the advantages that：
The test strips that are a kind of while detecting blood glucose and blood fat of the present invention have multiple reacting pads, can detect blood simultaneously
Sugar and blood fat realize integration detection.Blood glucose and blood fat are detected by single injected sampling simultaneously, reduce the volume of liquor sample,
Detection time is shortened, reduces the fussy degree of operation.It avoids and replaces trouble caused by test strips, glycosuria can be promoted
Patient identifies dyslipidemia and gives correct intervention early, can prevent atherosclerosis, reduce cardiocerebrovasculaevents events, reduces
The death rate.
Also, the test strips can also ensure that each sample-adding amount is the same by being loaded the limitation in space, it is ensured that
The high repeatability and precision of test strips.
A kind of while method that detects blood glucose and blood fat of the present invention, can by single injected sampling it is quick while detect blood
Sugar and blood fat reduce the fussy degree of operation.
Description of the drawings
Fig. 1 is the test strips schematic diagram in embodiment 1；
Fig. 2 is the test strips schematic diagram in embodiment 2.
Specific embodiment
Below in conjunction with the drawings and specific embodiments, the present invention will be described in detail.
Embodiment 1
Test strips 100 that are a kind of while detecting blood glucose and blood fat, the embodiment is using formula sample introduction of bleeding.The test strips 100
120, four first substrate layer 110 including closed assembly successively, insulating layer reacting pads（131,132,133,134）, second substrate layer
140th, flow deflector（151,152,153,154）, separating pad 160, diffusion layer 170 and cover plate 180, as shown in Figure 1.Wherein：
In the present embodiment, using polyethylene terephthalate（PET) sheet substrate as first substrate layer 110 with
Second substrate layer 140 recycles the mode of screen painting to form working electrode on first substrate layer 110（111,112,113,
114）, working electrode conducting wire, working electrode electrode tips connector（115,116,117,118）, which is used for
It is electrically connected with applying alive device, the shape paired electrode on second substrate layer 140（141,142,143,144）, it is right
Electrode cable, the electrode tips connector 149 to electrode, and setting second is opened on the position to four working electrodes of electrode face
Hole（145,146,147,148）Sample passes through for liquid, and for the working electrode with being carbon electrode to electrode, conducting wire is silver wire.
Insulating layer 120 between first substrate layer 110 and second substrate layer 140, and insulating layer 120 be equipped with it is anti-
It should pad（131,132,133,134）Matched first trepanning of shape（121,122,123,124）, which also exposes
The electrode tips connector of detecting electrode pair, for being electrically connected with applying alive electronic device.
Reacting pad（131,132,133,134）Contained reaction reagent includes electron transfer mediator, is reacted with liquor sample
And generate the enzyme and buffer solution of electric current.Wherein, electron transfer mediator is ferrocene.The enzyme of electric current is reacted and generated with liquor sample
For cholesterol esterase, cholesterol oxidase, catalase and glucose oxidase, it is respectively arranged on four reacting pads（131,
132,133,134）On.Buffer solution is TRIS buffer solutions.In this embodiment, some other substances can be also added in, such as
Triton X-100 etc., to improve the solubility of esters cholesterol.
Flow deflector（151,152,153,154）It is arranged on the second trepanning set on second substrate layer 140（145,146,
147,148）Top, just overlapped with second trepanning, separating pad 160 and diffusion layer 170 be then sequentially coated on second
On substrate layer 140, then plus cover plate 180, which is equipped with sample inlets 181.Lead between the different layers of the test strips
Adhesive is crossed to be bonded.
The test strips workflow of the present embodiment is as follows：
First, liquor sample is added dropwise to the sample inlets 181 on cover plate 180 in a manner of being added dropwise, by diffusion layer 170
Uniform diffusion is spread, and pass through flow deflector respectively around by 170 center of diffusion layer（151,152,153,154）, make liquid
Body sample passes through the separating pad 160 under diffusion layer 170, after being separated off chaff interferent by separating pad 160, liquor sample
Respectively by electrode（141,142,143,144）Second trepanning of upper setting（145,146,147,148）Into positioned at the second base
Reacting pad under plate layer 140（131,132,133,134）, in reacting pad（131,132,133,134）In, determinand respectively with
Cholesterol esterase, cholesterol oxidase, catalase or glucose oxidase reaction, generate electric current, instrument has been turned at this time
Capture program, but user is not prompted to start to detect, until the signal strength of acquisition is more than predetermined threshold, expression blood volume is filled just
Foot, instrument shows and user is reminded to start to measure at this time, and user stops that blood is added dropwise.Finally according to determinand in liquor sample
With differential responses pad（131,132,133,134）The size of current that middle enzyme reaction generates, obtain respectively in liquor sample blood glucose and
The content of blood fat.
Embodiment 2
As shown in Fig. 2, the test strips base of the test strips 200 and embodiment 1 of blood glucose and blood fat is detected while the present embodiment
This is identical, the difference lies in：
The test strips also closed assembly adhesive layer 290 and hydrophilic film 291 successively between diffusion layer and cover plate.The adhesive layer
290 are made by having sticking material, can adhere to hydrophilic film 291.The hydrophilic film 291 is by the material with hydrophilic radical
It is made, liquor sample can be sucked.
The adhesive layer is equipped with hollow-out parts 293, which is equipped with the siphon passage 293 at connection test strips edge；
The hydrophilic film 291 covers the hollow-out parts 292.
The test strips work of the present embodiment is essentially identical with the test strips workflow of embodiment 1, the difference lies in：
The test strips are by the way of siphon sample introduction, using the guiding of hydrophilic film 291, by liquor sample by siphon passage
293 are sucked into hollow-out parts 292, with the sample-adding amount of the space size limitation liquor sample of hollow-out parts 292, then make liquor sample by expanding
It dissipates layer 270 and uniformly diffuses to each reacting pad（231,232,233,234）, carry out subsequent reactions.
Embodiment described above only expresses the several embodiments of the present invention, and description is more specific and detailed, but simultaneously
Cannot the limitation to the scope of the claims of the present invention therefore be interpreted as.It should be pointed out that for those of ordinary skill in the art
For, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the guarantor of the present invention
Protect range.Therefore, the protection domain of patent of the present invention should be determined by the appended claims.
Claims (5)
1. test strips that are a kind of while detecting blood glucose and blood fat, which is characterized in that including substrate layer, reacting pad, flow deflector, separation
Pad, diffusion layer, adhesive layer, hydrophilic film and cover plate, the reacting pad are set in the substrate layer, the flow deflector, the separation
Pad, the diffusion layer, the adhesive layer, the hydrophilic film and the cover plate successively closed assembly in the substrate layer；The reacting pad
At least two, it is respectively provided with the enzyme of detection blood glucose and the enzyme for detecting blood fat thereon；At least two reacting pads
Connected with the diffusion layer；The substrate layer is equipped with detecting electrode pair, the quantity of the detecting electrode pair and the number of reacting pad
Amount is consistent, and each detecting electrode is to corresponding with a reacting pad；
The flow deflector is set between the reacting pad and the separating pad；
The middle part of the adhesive layer is equipped with hollow-out parts, which is equipped with the siphon passage at connection test strips edge, and described engraves
Empty portion corresponds to all reacting pad settings；The adhesive layer bonds the diffusion layer and the hydrophilic film, the hydrophilic film and institute
It states and the fixed sample-adding space of space size is formed between diffusion layer, the hydrophilic film covers the hollow-out parts；
For the detecting electrode to including the opposite working electrode in position and to electrode, the substrate layer includes first substrate layer and the
Two substrate layers, the first substrate layer are equipped with the electrode tips connector of working electrode, working electrode conducting wire and working electrode, this
Two substrate layers are equipped with the electrode tips connector to electrode, to electrode cable and to electrode；
Further include insulating layer, the insulating layer is set between first substrate layer and second substrate layer, the insulating layer be equipped with it is anti-
Matched first trepanning of pulvilliform shape is answered, which also exposes the electrode tips connector of the detecting electrode pair, so that described
Electrode tips connector is used to be electrically connected with applying alive electronic device.
2. test strips that are according to claim 1 while detecting blood glucose and blood fat, which is characterized in that described to be used to detect blood
The enzyme of fat is cholesterol esterase, cholesterol oxidase, peroxidase, catalase, lipoproteinesterase, glycerokinase, phosphorus
At least one of acid glycerol oxidizing ferment, the enzyme for being used to detect blood glucose is catalase, peroxidase, grape glycosyloxy
Change at least one of enzyme.
3. test strips that are according to claim 1 while detecting blood glucose and blood fat, which is characterized in that on the reacting pad also
Equipped with electron transfer mediator and buffer solution；The electron transfer mediator is ferricyanate, ferrocene or 1,4-benzoquinone；The buffer solution
For phosphate buffer, three (methylol) aminomethane-hydrochloride buffers or 2- (N- morpholines) ethanesulfonic acid buffer.
4. test strips that are according to claim 1 while detecting blood glucose and blood fat, which is characterized in that the substrate layer is poly-
Styrene, polyester, makrolon, polyethers, polyethylene, polypropylene, polyethylene terephthalate or poly terephthalic acid second two
Alcohol ester substrate layer.
5. method that is a kind of while detecting blood glucose and blood fat, it is characterised in that：Using test strips described in claim 1, by liquid
Body sample is added at siphon passage, and using the guiding of hydrophilic film, in a manner of siphon, liquor sample is sucked by siphon passage
Hollow-out parts with the sample-adding amount of the space size limitation liquor sample of hollow-out parts, then make liquor sample uniformly be spread by diffusion layer
To each reacting pad, liquor sample is on different reacting pads respectively with detecting the enzyme of blood glucose and detecting the enzyme reaction of blood fat, production
Raw electric current, according to size of current, obtains the content of blood glucose and blood fat in liquor sample respectively.
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| CN201310749689.3A CN104749228B (en) | 2013-12-30 | 2013-12-30 | The test strips and method of blood glucose and blood fat are detected simultaneously |
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|---|---|---|---|
| CN201310749689.3A CN104749228B (en) | 2013-12-30 | 2013-12-30 | The test strips and method of blood glucose and blood fat are detected simultaneously |
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| CN104749228B true CN104749228B (en) | 2018-07-06 |
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| CN106680347A (en) * | 2017-02-16 | 2017-05-17 | 常州鹤田生物技术有限公司 | Test paper for testing finger blood, and its application method and preparation method |
| CN108508074A (en) * | 2018-04-29 | 2018-09-07 | 浙江大学山东工业技术研究院 | A kind of anti-interference dual-port screen printing electrode |
| CN108398472A (en) * | 2018-04-29 | 2018-08-14 | 浙江大学山东工业技术研究院 | A kind of dual-port screen printing electrode having two-parameter detection function |
| CN109916894A (en) * | 2019-04-12 | 2019-06-21 | 吉林省汇酉生物技术股份有限公司 | A kind of dry chemistry reagent piece and preparation method thereof quantitative determining concentration of glucose |
| CN109916890A (en) * | 2019-04-12 | 2019-06-21 | 吉林省汇酉生物技术股份有限公司 | A kind of dry chemistry reagent piece and preparation method thereof quantitative determining total cholesterol concentration |
| US20220218240A1 (en) * | 2019-08-19 | 2022-07-14 | Medtrum Technologies Inc. | Sensing device |
| CN110568045A (en) * | 2019-09-12 | 2019-12-13 | 浙江大学山东工业技术研究院 | Electrochemical biosensor capable of rapidly detecting |
| CN112029821A (en) * | 2020-08-18 | 2020-12-04 | 美康生物科技股份有限公司 | Blood fat test card and application thereof |
| CN113109405B (en) * | 2021-04-01 | 2022-04-15 | 广州南雪医疗器械有限公司 | Test paper for detecting triglyceride |
| CN113219168A (en) * | 2021-05-06 | 2021-08-06 | 黄波 | Colloidal gold test strip |
| CN114798020B (en) * | 2022-03-28 | 2024-07-09 | 四川成电医联科技咨询有限公司 | Multichannel electrochemical microfluidic test card and manufacturing method thereof |
| CN115684611A (en) * | 2022-12-30 | 2023-02-03 | 天津德祥生物技术股份有限公司 | Erythrocyte antigen detection test strip and application |
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