CN201825958U - Cell culture solution flowing supply device - Google Patents
Cell culture solution flowing supply device Download PDFInfo
- Publication number
- CN201825958U CN201825958U CN2010205797243U CN201020579724U CN201825958U CN 201825958 U CN201825958 U CN 201825958U CN 2010205797243 U CN2010205797243 U CN 2010205797243U CN 201020579724 U CN201020579724 U CN 201020579724U CN 201825958 U CN201825958 U CN 201825958U
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- CN
- China
- Prior art keywords
- cell culture
- culture fluid
- culture solution
- cell
- supply device
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- Expired - Fee Related
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Abstract
The utility model provides a cell culture solution flowing supply device comprising a cell exposure experiment cavity and a cell culture solution reception cavity, wherein a microporous membrane plate special for experimental cells is placed between the two cavities; the exteriors of the two cavities are provided with cell culture solution temperature bath devices; and one side of the cell culture solution reception cavity is provided with a cell culture solution supply device, and the other side of the cell culture solution reception cavity is provided with a cell culture solution diversion discharge pipe. The device has the benefit of providing a new cell culture solution continuous supply mode. In a cell exposure contamination experiment, the device not only can ensure enough nutrition for the survival and the normal growing of the cells, but also can prevent the whole cells from being immersed into cell culture solution to influence the experiment effect, and is suitable for long-time exposure experiments. The cell culture solution flowing supply device can be widely applied to the fields of medical science, public hygienic environmentology, molecular biology, cytology, toxicology and the like, and can ensure the normal viability of the cells when being used for replacing living animals for contamination experiments.
Description
Technical field
The utility model relates to the mobile feeding mechanism of a kind of cell culture fluid.
Background technology
In each field such as medical science, public hygienic environment, molecular biology, cytology, toxicology, carry out direct experiment through regular meeting's pair cell.But how in experiment, to keep cytoactive normal, both guaranteed that cell survived and the needed sufficient nutrition of normal growth, cell integral body can be immersed in again influences test effect to adapt to long exposure experiment in the cell culture fluid, become a maximum difficult problem.
The utility model content
For addressing the above problem, the utility model provides a kind of cell culture fluid feeding mechanism that flows, when keeping cell culture fluid to soak into micropore lamina membranacea bottom surface constantly offering experimental cell growth desired nutritional, can derive the assurance cell culture fluid at any time again, make its upper surface be no more than cell upper surface on the micropore template, promptly can have sufficient nutrient can be directly exposed to the long-time exposure experiment of reception test material in the cellular exposure chamber again to guarantee cell.
For achieving the above object, the technical solution of the utility model provides the mobile feeding mechanism of a kind of cell culture fluid, comprises cellular exposure experiment chamber and cell culture fluid receptive cavity, places the micropore lamina membranacea of experimental cell special use between two cavitys.Two cavitys have the cell culture fluid warm bath apparatus outward.Cell culture fluid receptive cavity one side is the cell cultures liquid supply unit, and opposite side is a cell culture fluid water conservancy diversion delivery pipe.
Described cell culture fluid warm bath apparatus is bathed the chamber by temperature and the temperature that links to each other by conduit is with it bathed control device and formed, and what guarantee culturing cell normally survives the needed temperature of growing.
Nutrient solution flow director dominant discharge is arranged on the described cell cultures liquid supply unit, and by the current stabilization peristaltic pump in the cell culture fluid receptive cavity quantitatively and stable conveying cell culture fluid, what guarantee culturing cell normally survives the needed nutrition of growth.
Effect of the present utility model is: provide a kind of new cell culture fluid to continue method of supplying.Both guaranteed in the cellular exposure exposure experiment that cell survived and the needed sufficient nutrition of normal growth, cell integral body can be immersed in again influences test effect in the cell culture fluid, and is fit to long exposure test.Can be widely used in each field such as public hygienic environment, molecular biology, cytology, toxicology, carry out guaranteeing in the exposure experiment that at alternative traditional living animal cytoactive is normal.
Description of drawings
Accompanying drawing is the synoptic diagram of the utility model structure.
Among the figure:
1, cellular exposure experiment chamber
2, micropore lamina membranacea
3, cell culture fluid water conservancy diversion delivery pipe
4, cell culture fluid receptive cavity
5, temperature is bathed the chamber
6, current stabilization peristaltic pump
7, nutrient solution flow director
8, cell cultures liquid supply unit
9, temperature is bathed control device
10, conduit
11, temperature is bathed control device
In conjunction with the accompanying drawings the utility model is illustrated.
As shown in drawings, place the micropore lamina membranacea (2) of experimental cell special use between cellular exposure experiment chamber (1) and these two cavitys of cell culture fluid receptive cavity (4).Two cavitys have cell culture fluid warm bath apparatus (11) outward.Cell culture fluid receptive cavity (4) one sides are cell cultures liquid supply unit (8), and opposite side is a cell culture fluid water conservancy diversion delivery pipe (3).Cell culture fluid warm bath apparatus (11) is bathed chamber (5) by temperature and is bathed control device (9) by the continuous temperature of conduit (10) with it and form.Nutrient solution flow director (7) dominant discharge is arranged on the cell cultures liquid supply unit (8), and, keep cell culture fluid to soak into micropore lamina membranacea bottom surface constantly and offer the required nutrition of experimental cell growth by current stabilization peristaltic pump (6) quantitative and stable conveying cell culture fluid in cell culture fluid receptive cavity (4).Cell culture fluid water conservancy diversion delivery pipe (3) is derived cell culture fluid at any time and is guaranteed that the cell culture fluid upper surface is no more than the cell upper surface on the micropore template simultaneously, and cell promptly can have sufficient nutrient can be directly exposed to the long-time exposure experiment of the interior reception test material in cellular exposure experiment chamber (1) again.
When using, at first open micropore lamina membranacea (2) that cellular exposure experiment chamber (1) will have experimental cell with cultivating and after being placed under the aseptic condition on the cell culture fluid receptive cavity (4), steadily putting well, cellular exposure is tested chamber (1) and cover completely.Regulating nutrient solution flow director (7) makes nutrient solution just reach micropore template lower surface to have and can not flood the experimental cell upper surface.Current stabilization peristaltic pump (6) is started working, and makes stable being fed in the cell culture fluid receptive cavity (4) of cell culture fluid, opens cell culture fluid water conservancy diversion delivery pipe (3) simultaneously, to form the mobile supply of a cell culture fluid, keeps the freshness of cell culture fluid.Open temperature and bathe control device (9), guarantee that temperature bath chamber guarantees experimental cell normal growth under 37 ℃ temperature condition all the time.Can close nutrient solution flow director (7), current stabilization peristaltic pump (6) and temperature after experiment is finished and bathe control device (9).Open micropore lamina membranacea (2) that cellular exposure experiment chamber (1) will have experimental cell and under aseptic condition, takes out the biological phenomena and the detection of the correlated results aspect the biology of the processes such as growth, breeding, cytodifferentiation and cell aging of carrying out observation of cell.
Claims (3)
1. the cell culture fluid feeding mechanism that flows, it is characterized in that: comprise cellular exposure experiment chamber (1) and cell culture fluid receptive cavity (4), place the micropore lamina membranacea (2) of experimental cell special use between two cavitys, two cavitys have cell culture fluid warm bath apparatus (11) outward, cell culture fluid receptive cavity (4) one sides are cell cultures liquid supply unit (8), and opposite side is a cell culture fluid water conservancy diversion delivery pipe (3).
2. the cell culture fluid according to claim 1 feeding mechanism that flows, it is characterized in that: described cell culture fluid warm bath apparatus (9) is bathed chamber (5) by temperature and the temperature that links to each other by conduit (10) is with it bathed control device (11) and formed, the assurance culturing cell normally survive the needed temperature of growing.
3. the cell culture fluid according to claim 1 feeding mechanism that flows, it is characterized in that: nutrient solution flow director (7) dominant discharge is arranged on the described cell cultures liquid supply unit (8), and by current stabilization peristaltic pump (6) in cell culture fluid receptive cavity (4) quantitatively and stable conveying cell culture fluid, what guarantee culturing cell normally survives the needed nutrition of growth.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010205797243U CN201825958U (en) | 2010-10-28 | 2010-10-28 | Cell culture solution flowing supply device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010205797243U CN201825958U (en) | 2010-10-28 | 2010-10-28 | Cell culture solution flowing supply device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201825958U true CN201825958U (en) | 2011-05-11 |
Family
ID=43964508
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010205797243U Expired - Fee Related CN201825958U (en) | 2010-10-28 | 2010-10-28 | Cell culture solution flowing supply device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201825958U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102010823A (en) * | 2010-10-28 | 2011-04-13 | 天津开发区合普工贸有限公司 | Cell culture solution flow supply device |
-
2010
- 2010-10-28 CN CN2010205797243U patent/CN201825958U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102010823A (en) * | 2010-10-28 | 2011-04-13 | 天津开发区合普工贸有限公司 | Cell culture solution flow supply device |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20110511 Termination date: 20161028 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |