CN201406429Y - Photo-bioreactor capable of transforming CO2 effectively - Google Patents

Photo-bioreactor capable of transforming CO2 effectively Download PDF

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Publication number
CN201406429Y
CN201406429Y CN 200920085779 CN200920085779U CN201406429Y CN 201406429 Y CN201406429 Y CN 201406429Y CN 200920085779 CN200920085779 CN 200920085779 CN 200920085779 U CN200920085779 U CN 200920085779U CN 201406429 Y CN201406429 Y CN 201406429Y
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gas
tank body
glass tank
bioreactor
communicated
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吴高明
杨忠华
刘汉杰
陈明明
王剑
李轩科
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Wuhan Iron and Steel Group Corp
Wuhan University of Science and Engineering WUSE
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Wuhan Iron and Steel Group Corp
Wuhan University of Science and Engineering WUSE
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02—Photobioreactors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/18—External loop; Means for reintroduction of fermented biomass or liquid percolate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/26—Conditioning fluids entering or exiting the reaction vessel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes

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Abstract

The utility model relates to a photo-bioreactor capable of transforming CO2 effectively, which solves the problems that the existing reactor has complex structure, bad gas-liquid mixing efficiency andlow rate by using microalgae to fix the CO2. The technical scheme is characterized in that the photo-bioreactor comprises a glass tank body which is provided with a culture medium inlet, a culture medium outlet, a cooled water inlet, a cooled water outlet, an air inlet and an air outlet; multi-group heat exchanging pipes communicated with the cooled water inlet and the cooled water outlet are circlewise arranged at the internal side of the glass tank body; the glass tank body is divided into an ascending area and a descending area by an ascending conduit; the top of the glass tank body is also provided with a gas separating area; the air inlet is communicated with a gas distributor at the bottom of the ascending area by an air inlet pipeline; the descending area is provided with a cultureliquid external circulation pipeline; and the culture liquid external circulation pipeline is communicated with the air inlet pipeline by a water pump. The photo-bioreactor has simple structure, is simple and convenient for operation, is particularly applied to that industrial effluent gas utilizes the photosynthesis of the microalgae to fix CO2 effectively in an exhaust-reducing system, leads the exhaust-reducing efficiency on the CO2 to achieve more than 50%, and has low operation cost and short period.

Description

A kind of efficient conversion CO 2Bioreactor
Technical field
The utility model relates to and a kind ofly is used for algae and carries out photosynthetic efficient conversion CO 2Bioreactor.
Background technology
The bio-reactor that is used for little algae processing at present has various structures, consider that little algae is relatively more responsive to high-shear, usually select the bioreactor of air lift type, though but such bioreactor adopts gas-liquid circulation blended method, but still exist gas-liquid mixed efficient not high, mass transfer intensity problem of smaller, for strengthening gas-liquid mixed and mass transfer, the technician has done various improvement, as in the patent No. 200420009076.2, name is called in the utility model patent of " airlift photobioreactor ", for addressing the above problem, the nozzle quantity and the shape of gas distributor has been done improvement, in the hope of being more conducive to the dispersion of gas in nutrient solution, eliminate the dead angle.But in the practical application, how to design as the structure of opinion to gas distributor, its gas-liquid mixed improved efficiency is also not obvious, mainly is because gas and liquid only carry out one time gas-liquid mixed in tank body, mass transfer area is subjected to the restriction of bubble size, thereby influences mass-transfer efficiency.Certainly for the common airlift photobioreactor that is used to cultivate little algae, because gas concentration lwevel lower (about 5% volume) in the gas of its feeding, so this class is improved and can be met the demands substantially.But when the industrial discharge gas that needs processing gas concentration lwevel height (about 20% volume) carried out carbon dioxide discharge-reduction, except that technology was optimized, gas-liquid mixed and mass transfer were for little algae fixation of C O efficiently 2Speed have great effect equally, and above-mentioned conventional the improvement is difficult to meet the demands.
Summary of the invention
The purpose of this utility model is in order to solve the problems of the technologies described above, provide a kind of simple in structure, easy to operate, have high efficient gas and liquid mixing efficiency, reinforcing mass transfer, an efficient conversion CO that the circulation degree is high 2Bioreactor, this reactor can well be used to utilize little algae to containing high concentration CO 2Industrial discharge gas handle photosynthesis.
Technical solutions of the utility model comprise the glass tank body that is provided with substratum import, nutrient solution outlet, entrance of cooling water, cooling water outlet, inlet mouth and venting port, described glass tank body interior annular is provided with the many heat transfer tubes that are communicated with entrance of cooling water and cooling water outlet respectively, under being divided into the glass tank body, riser duct rises the district and the district that descends, the gas delivery district is also established at glass tank body top, and inlet mouth is communicated with the gas distributor that is positioned at the bottom, rising area through intake ducting.
Described decline district is provided with the nutrient solution external circulation line, and described nutrient solution external circulation line is communicated with intake ducting through water pump.
Be provided with Venturi tube between described inlet mouth and the intake ducting, nutrient solution is communicated with intake ducting through Venturi tube.
Rise the district and the district that descends under by riser duct the glass tank body being divided into, gas distributor is located at the bottom, rising area, ejection contains a large amount of pending gases nutrient solution of (pending gas refers to that the carbon dioxide content volumetric concentration is up to 20% the gas that little algae transforms that is used in the utility model), because bottom, rising area gas holdup is big, so its density is little, nutrient solution is light, add jet flow kinetic energy, liquid in the upcast is risen, when arriving top, rising area, because upper space is big, gas is escaped out from nutrient solution, and the district is discharged by venting port by gas delivery, and nutrient solution becomes heavily from the district's decline that descends simultaneously, circulate again during to reactor bottom and enter the rising area, form circulation repeatedly.
Simultaneously, the contriver is on the basis of having studied existing various air lift type photoreactor structures, courageously design, broken through the notion of only considering how in the glass tank body, to strengthen gas-liquid mixed at present, extract the intravital part nutrient solution of glass pot out through water pump by the nutrient solution external circulation line in the district descending, carry out the gas-liquid mixed in early stage earlier at the external and pending gas of glass pot, and then send into and carry out little algae in the glass tank body of bioreactor of the present utility model and transform, like this, except glass pot culturing in vivo liquid the circulation of rising area and last transition, also make up the external reflux loop structure of band Venturi tube getter device in the glass pot external body, strengthen the circulation degree by interior outer circulation, be beneficial to gas-liquid mixed.
Further, invent everybody and also between inlet mouth and intake ducting, adopted Venturi tube, when nutrient solution is sent into Venturi tube, utilize the flow velocity of liquid in the Venturi meter contraction section to increase the principle that pressure diminishes, form negative pressure pending gas is sucked Venturi tube by inlet mouth, and bubble is disperseed and with the liquid uniform mixing, through in Venturi tube, carrying out fully gas-liquid mixed in earlier stage, send into gas distributor together by intake ducting again, the nutrient solution of gas distributor ejection contains a large amount of pending gases, and gas mainly exists with the micro-bubble form, to increase mass transfer area, can obtain mass transfer effect efficiently.
Advantage of the present utility model is:
1, the utility model is simple in structure, easy and simple to handle, be specially adapted to carry out utilizing in the carbon dioxide discharge-reduction system little algae to carry out photosynthesis with efficient stabilizing carbon dioxide at industrial discharge gas, reduction of discharging speed to carbonic acid gas can reach more than 50%, and running cost is low, the cycle is short.
2, by setting up two cover circulations of glass pot inside and outside, pending gas and nutrient solution have carried out the gas-liquid mixed in early stage, have further strengthened the circulation degree.
3, utilize the Venturi tube own characteristic, make nutrient solution and pending gas thorough mixing in pipe, bubble fully disperse and with the liquid uniform mixing, again when gas distributor sprays, gas mainly exists with micro-bubble shape in the glass tank body, effectively improved mass transfer area, liquid mixing is good, mass transfer intensity height.
Description of drawings
Fig. 1 is a structural representation of the present utility model.
Fig. 2 is gas CO in the venting port after treatment 2The concentration synoptic diagram.
The import of 1-substratum, the outlet of 2-nutrient solution, 3-entrance of cooling water, 4-cooling water outlet, 5-inlet mouth, 6-venting port, 7-glass tank body, 8-heat transfer tube, 9-riser duct, 10-rising area, 11-decline district, 12-gas delivery district, 13-intake ducting, 14-gas distributor, 15-nutrient solution external circulation line, 16-water circulating pump, 17-Venturi tube.
Embodiment
Below in conjunction with accompanying drawing the utility model is further explained explanation:
With reference to Fig. 1, the upper end of the glass tank body 7 of the utility model bioreactor is provided with venting port 6, substratum import 1, and the lower end is provided with nutrient solution outlet 2.Glass tank body 7 interior annular be provided with polycomponent not with glass tank body 7 on entrance of cooling water 3 and the heat transfer tube 8 that is communicated with of cooling water outlet 4, described heat transfer tube 8 can be snakelike or other shape; Medullary ray along glass tank body 7 is provided with riser duct 9, riser duct 9 can be fixed on the glass tank body 7 by support, its inside is that rising area 10, outside are the district 11 that descends, 10 bottoms are provided with the gas distributor 14 that is communicated with intake ducting 13 exit end in the rising area, the entrance end of described intake ducting 13 is provided with Venturi tube 17, inlet mouth 12 connects the inlet pipe of Venturi tube 17, described decline district 11 also is provided with nutrient solution external circulation line 15, and described nutrient solution external circulation line 15 connects the liquid-inlet pipe of Venturi tube 17 through water circulating pump 16.
The principle of work of the utility model bioreactor is:
Adopt CO in the present embodiment 2The industrial discharge gas of concentration about 25% is met the CO of little algae demand after dedusting, washing, pressurization, regulating the qi flowing in the channels 2Concentration reaches the pending gas of 20% (volume percent); Described little algae can be selected algae chlorella (Chlorella vulgarisWUST 11, number of patent application 200810197667.X) for use.
Pending gas is because negative pressure is inhaled into Venturi tube 17 by inlet mouth 12, the nutrient solution thorough mixing that in Venturi tube 17, comes with circulating by glass tank body 7, be sprayed on rising area 10 by gas distributor 14 again, gas flow is 0.1-0.5vvm (amount that feeds gas for per minute is the multiple of liquid) with respect to the bioreactor volume, because gas-liquid carried out mixing in earlier stage in Venturi tube 17, connect gas distributor 14 by intake ducting 13 again, gas distributor 14 ejections contain the nutrient solution of a large amount of pending gases, gas exists with very small bubble form, can obtain mass transfer area very efficiently, pending gas is uniform distribution in glass tank body 7, the violent mixing to promote CO in the gas 2Mass transfer, be dissolved in the CO in the nutrient solution 2Be converted into the little algal biomass that utilizes value by little algae by photosynthesis, discharge O simultaneously 2, fixation of C O 2The O that produces in the process 2, the rare gas element that do not utilize, as nitrogen etc., and loose CO 2Discharge through the venting port 13 of glass tank body 7 upper ends by gas delivery district 19.Adding the reaction volume device at the bioreactor initial operating stage is the algae kind seed liquor of 20% volume, add fresh training base continuously by substratum import 1 behind the steady operation, simultaneously at the nutrient solution of emitting same traffic by nutrient solution outlet 2 continuously, thinning ratio is 0.5/d (the reactor internal volume is divided by flow rate when flowing to reactor for liquid), makes entire work process be in stable volume and stable frond biological concentration (OD 685Value can reach 2.8).Contain fixation of C O in the nutrient solution of emitting 2The biomass that generate in the process are discharged through nutrient solution outlet 2 with nutrient solution.The control of the temperature of bioreactor realizes by its inner heat transfer tube that is provided with 8, feeds heat transferring medium in the pipe, carries out heat exchange with the nutrient solution of glass tank body 7 inside, realizes temperature control by the flow of heat transferring mediums in the control heat transfer tube 8.Luminous energy is provided by sunlight, mainly absorbs by tank skin around the glass tank body 7, can consider simultaneously to strengthen solar light collection and assimilated efficiency by the sunlight baffle is set.
Fig. 2 has provided employing present embodiment method and has handled gas CO in the rear exhaust port 2Concentration.By among the figure as can be known, initial CO 2The stack gas of concentration about 25% after the utility model method is handled, the CO of venting port gas 2Concentration is reduced to 10% (volume percent), handles composition complexity like this, high concentration CO 2During the gas of content, system still can be stable, CO 2Reducing discharging speed reaches more than 50%.

Claims (3)

1, a kind of efficient conversion CO 2Bioreactor, comprise the glass tank body that is provided with substratum import, nutrient solution outlet, entrance of cooling water, cooling water outlet, inlet mouth and venting port, it is characterized in that, described glass tank body interior annular is provided with the heat transfer tube that polycomponent is not communicated with entrance of cooling water and cooling water outlet, under being divided into the glass tank body, riser duct rises the district and the district that descends, the gas delivery district is also established at glass tank body top, and inlet mouth is communicated with the gas distributor that is positioned at the bottom, rising area through intake ducting.
2, efficient conversion CO as claimed in claim 1 2Bioreactor, it is characterized in that described decline district is provided with the nutrient solution external circulation line, described nutrient solution external circulation line is communicated with intake ducting through water pump.
3, efficient conversion CO as claimed in claim 1 or 2 2Bioreactor, it is characterized in that be provided with Venturi tube between described inlet mouth and the intake ducting, the nutrient solution external circulation line is communicated with intake ducting through Venturi tube.
CN 200920085779 2009-05-15 2009-05-15 Photo-bioreactor capable of transforming CO2 effectively Expired - Lifetime CN201406429Y (en)

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010102329A1 (en) * 2009-03-09 2010-09-16 Ian Malcolm Wright A bioreactor
CN101550394B (en) * 2009-05-15 2011-07-20 武汉钢铁(集团)公司 Airlift type photobioreactor
CN102311923A (en) * 2010-07-07 2012-01-11 中国石油化工股份有限公司 Microalgae cultivation method
CN102311924A (en) * 2010-07-07 2012-01-11 中国石油化工股份有限公司 Method for open-type culture of microalgae
CN102668928A (en) * 2012-04-05 2012-09-19 山东纯英光电科技有限公司 Underwater LED photosynthetic bioreactor
CN102796655A (en) * 2012-09-07 2012-11-28 林艳 Air-compression-free aerobic fermentation tank
CN103173352A (en) * 2013-03-15 2013-06-26 博赢（昆山）生物科技有限公司 Device for culturing hair weed cells on large scale

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010102329A1 (en) * 2009-03-09 2010-09-16 Ian Malcolm Wright A bioreactor
CN101550394B (en) * 2009-05-15 2011-07-20 武汉钢铁(集团)公司 Airlift type photobioreactor
CN102311923A (en) * 2010-07-07 2012-01-11 中国石油化工股份有限公司 Microalgae cultivation method
CN102311924A (en) * 2010-07-07 2012-01-11 中国石油化工股份有限公司 Method for open-type culture of microalgae
CN102311923B (en) * 2010-07-07 2013-04-10 中国石油化工股份有限公司 Microalgae cultivation method
CN102668928A (en) * 2012-04-05 2012-09-19 山东纯英光电科技有限公司 Underwater LED photosynthetic bioreactor
CN102796655A (en) * 2012-09-07 2012-11-28 林艳 Air-compression-free aerobic fermentation tank
CN103173352A (en) * 2013-03-15 2013-06-26 博赢（昆山）生物科技有限公司 Device for culturing hair weed cells on large scale

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Granted publication date: 20100217

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