CN201321453Y - Two-way device for collecting magnetotactic bacteria - Google Patents
Two-way device for collecting magnetotactic bacteria Download PDFInfo
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- CN201321453Y CN201321453Y CN 200820123577 CN200820123577U CN201321453Y CN 201321453 Y CN201321453 Y CN 201321453Y CN 200820123577 CN200820123577 CN 200820123577 CN 200820123577 U CN200820123577 U CN 200820123577U CN 201321453 Y CN201321453 Y CN 201321453Y
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
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Abstract
The utility model provides a two-way device for collecting magnetotactic bacteria, and relates to the bacterium-collecting technology. A collector of the device is a container with an opening formed at the upper end of the container; openings are formed on the opposite side walls of the container; a tubular separating tube is arranged at the inner part of each opening; a sample tube is sleeved at the outer end of each separating tube; the two sample tubes are communicated with an inner cavity of the collector through the two separating tubes; the collector is arranged between two Helmholtz coils which are opposite in parallel and vertically arranged; the two separating tubes on the collector are respectively opposite to the two coils; and the axial line of the seperating tube is consistent with the direction of a magnetic line; and the two coils are electrically connected with a controller. By applying uniform weak field on a sample containing the magnetotactic bacterium, the two-way device can collect the magnetotactic bacteria which move along and against the magnetic line at the same time when the magnetic field ranges from 0.1 hauteur to 1 hauteur, and can quickly and effectively collect maximum magnetotactic bacterium from the environment without preference. The device has the advantages of simple structure, low cost and convenient use, and provides high-quality equipment for the study on the diversity and the magnetic property of the magnetotactic bacterium.
Description
Technical field
The utility model relates to bacterium collection technique field, be a kind of by uniform magnetic field, from environment the novel two-way collection device of separation and purification magnetotactic bacteria.
Background technology
Magnetotactic bacteria (Magnetotactic bacteria) is the gram negative bacterium that a class can be moved along field direction, and they are widely distributed, can both find in lake, ocean even wet soil.The formation of magnetotactic bacteria magnetic corpusculum and the research of magnetic property not only help to disclose the process of biomineralization, the mechanism that discussion biological response terrestrial magnetic field changes, and also are the important research contents of archaeomagnetism, rock magnetism and environmental magnetism.In addition, bacteriogenic magnetite is also demonstrating outstanding glamour aspect modern biology and the medical use.Therefore, since the nineties in 20th century, the research of magnetotactic bacteria has been subjected to all multidisciplinary extensive concerns.Referring to document " biomineralization of magnetotactic bacteria magnetic corpusculum and magnetic property progress " " Science Bulletin,, the 49th volume, the 24th phase, p2505-2510 in 2004 "; Document " magnetotactic bacteria magnetic corpusculum progress " " microbiology circular,, the 33rd volume, the 3rd phase, p133-137 in 2006 ".
But most magnetotactic bacterias are anaerobism or little aerobic type bacterium, therefore are difficult in and realize pure culture in the laboratory.So except the bacterium that only a few can be cultivated, other magnetotactic bacterias that are used for scientific research are all directly collected from environment.At present, the method for separating magnetotactic bacteria from environment commonly used is unidirectional collecting method, promptly according to the magnetotactic characteristic of bacterium, by sample is applied magnetic field, collect the magnetotactic bacteria that moves about along the magnetic line of force direction.But these methods exist two distinct disadvantage: the one, can not collect bacterium against the magnetic line of force motion, and increasing observation and test-results show, exist bacterium a large amount of, contrary magnetic line of force motion in environment; The 2nd, bacterium residing terrestrial magnetic field in environment is low intensive uniform field (it is special to be about 0.05 milli), and traditional method applies sample is high-intensity gradient fields, these externally-applied magnetic fields can produce adverse influence to magnetotactic bacteria, and then can have influence on the effect of collection.
The utility model content
In order to overcome the deficiency of traditional device for collecting magnetotactic bacteria, the purpose of this utility model is: disclose a kind of two-way device for collecting magnetotactic bacteria, this device can not only produce more weak uniform magnetic field (magnetic field range is special special to 1 milli from 0.1 milli), and can collect simultaneously along with the magnetotactic bacteria that moves against magnetic line of force.
For achieving the above object, technical solution of the present utility model is:
A kind of two-way device for collecting magnetotactic bacteria comprises collector, separator tube, sample hose, Helmholtz coil and coil controller; Its collector is the container that there is an opening upper end, respectively is provided with an opening on relative two walls of container side wall, the separable affixed tubulose separator tube of difference in two openings; Each overcoat one sample hose of two separator tube outer ends, two sample hoses communicate with the inner chamber of collector through separator tube;
Collector places between a pair of Helmholtz coil, and two Helmholtz coils are parallel to be provided with relatively and vertically; Two tubulose separator tubes on the collector make the axis of the two tubulose separator tubes that stretch out consistent with the magnetic line of force direction respectively over against a Helmholtz coil, and two Helmholtz coils are electrically connected with controller respectively.
Described two-way device for collecting magnetotactic bacteria is placed the filter pulp plug in the inner pipe of its described two tubulose separator tubes, and outer end opening seals with rubber plug, and there is through hole at the rubber plug center.
Described two-way device for collecting magnetotactic bacteria, the magnetic field size that its described two Helmholtz coils are produced is regulated by controller.
Described two-way device for collecting magnetotactic bacteria, the magnetic field that its described two Helmholtz coils are produced is uniform magnetic field, the magnetic field size is special between the 1 milli spy at 0.1 milli.
Described two-way device for collecting magnetotactic bacteria, when it used, the collector inner chamber was marked with sample and sterilized water; Fill with disinfectant river or seawater in two tubulose separator tubes and two sample hoses.
Described two-way device for collecting magnetotactic bacteria, the height of two sidewall openings of its described sample and sterilized water elevation of water surface 〉=collector.
Described two-way device for collecting magnetotactic bacteria, its described two sample hoses communicate with the inner chamber of collector through separator tube, are that two sample hoses are through the rubber plug central through hole, communicate with the inner chamber of collector through separator tube, filter pulp plug again.
The beneficial effects of the utility model are, can be from environment no preference ground, fast and effeciently along with the magnetotactic bacteria that separates and collect maximum against the magnetic line of force direction; Collection device is simple in structure, and is with low cost, easy to use.
Description of drawings
Fig. 1 is a two-way device for collecting magnetotactic bacteria structural representation of the present utility model;
Fig. 2 is a two-way device for collecting magnetotactic bacteria middle controller circuit diagram of the present utility model.
Embodiment
The utility model is described in further detail below in conjunction with accompanying drawing.
Two-way device for collecting magnetotactic bacteria structure of the present utility model is as shown in Figure 1: comprise collector 1, separator tube 2, sample hose 5, a pair of Helmholtz coil 6 and coil controller 7.Collector 1 has the container of an opening for the upper end, respectively be provided with an opening on relative two walls of container side wall, place filter pulp plug 3 in a separable respectively affixed tubulose separator tube 2, the two tubulose separator tubes 2 inner pipes in two openings, outer end opening seals with rubber plug 4, and there is through hole at rubber plug 4 centers; Each overcoat one sample hose 5, two sample hose 5 of two separator tubes, 2 outer ends are through rubber plug 4 central through holes, communicate with the inner chamber of collector 1 through separator tube 2, filter pulp plug 3 again.
The circuit of two-way device for collecting magnetotactic bacteria middle controller of the present utility model as shown in Figure 2, comprise rheometer 71, coil interface 72, slide rheostat 73, switch 74 and power supply 75, be connected into loop between each parts mutually, be electrically connected with two Helmholtz coils 6 respectively with two contacts of coil interface 72.
Two-way device for collecting magnetotactic bacteria working process of the present utility model, use and precaution are as follows.
Working process of the present utility model: the sample that will contain magnetotactic bacteria is put into the cavity volume of collector 1, magnetotactic bacteria is under the effect that adds uniform magnetic field, pass filter pulp plug 3 by separator tube 2 from the sidewall opening of collector 1, and pass rubber plug 4 central through holes and swim in the sample hose 5, along entering two sample hoses 5 respectively, thereby reach the purpose that two-way simultaneous is collected magnetotactic bacteria with the magnetotactic bacteria that moves against magnetic line of force.
Use of the present utility model:
1. place filter pulp plug 3 respectively in two separator tube 2 the inners of collector 1;
2. in sample hose 5, fill with disinfectant river or seawater (originate per sample and decide);
3. two separator tubes, 2 outer end openings are sealed with rubber plug 4, each overcoat one sample hose 5 in two separator tubes, 2 outer ends interlinks two separator tubes 2 and two sample hoses 5 again, fills with sterilization river or seawater in two separator tubes 2 after connecting;
4. connect the separator tube 2 of sample hose 5 with two again and the two side opening of collector 1 links to each other, in collector 1, add sample and the sterilized water that contains magnetotactic bacteria then;
5. whole collection device is put between two Helmholtz coils 6, the axis that makes the two tubulose separator tubes 2 that stretch out is with after the magnetic line of force direction is consistent, open controller 7 power supplys, make two Helmholtz coils 6 produce uniform magnetic field, the controlling magnetic field scope is special between 1 milli spy at 0.1 milli, begins to collect magnetotactic bacteria then.
Application notice of the present utility model:
1. note from sample hose 5, rubber plug 4, separator tube 2 must be unimpeded to the whole connection of collector 1, avoids producing bubble;
2. in collector 1, slowly add sample, avoid sample to stop up the sidewall opening of collector 1;
3. the sample of injection collector 1 and sterilized water elevation of water surface should surpass the height of two sidewall openings;
4. avoid running gear after beginning to collect;
5. collection time was generally 1 hour.
Claims (7)
1, a kind of two-way device for collecting magnetotactic bacteria comprises collector, separator tube, sample hose, Helmholtz coil and coil controller; It is characterized in that collector is the container that there is an opening upper end, respectively be provided with an opening on relative two walls of container side wall, the separable affixed tubulose separator tube of difference in two openings; Each overcoat one sample hose of two separator tube outer ends, two sample hoses communicate with the inner chamber of collector through separator tube;
Collector places between a pair of Helmholtz coil, and two Helmholtz coils are parallel to be provided with relatively and vertically; Two tubulose separator tubes on the collector make the axis of the two tubulose separator tubes that stretch out consistent with the magnetic line of force direction respectively over against a Helmholtz coil, and two Helmholtz coils are electrically connected with controller respectively.
2, two-way device for collecting magnetotactic bacteria as claimed in claim 1 is characterized in that, places the filter pulp plug in the inner pipe of described two tubulose separator tubes, and outer end opening seals with rubber plug, and there is through hole at the rubber plug center.
3, two-way device for collecting magnetotactic bacteria as claimed in claim 1 is characterized in that, the magnetic field size that described two Helmholtz coils are produced is regulated by controller.
4, two-way device for collecting magnetotactic bacteria as claimed in claim 1 is characterized in that, the magnetic field that described two Helmholtz coils are produced is uniform magnetic field, and the magnetic field size is special between the 1 milli spy at 0.1 milli.
5, two-way device for collecting magnetotactic bacteria as claimed in claim 1 is characterized in that, during use, the collector inner chamber is marked with sample and sterilized water; Fill with disinfectant river or seawater in two tubulose separator tubes and two sample hoses.
6, two-way device for collecting magnetotactic bacteria as claimed in claim 5 is characterized in that, the height of two sidewall openings of described sample and sterilized water elevation of water surface 〉=collector.
7, two-way device for collecting magnetotactic bacteria as claimed in claim 1 or 2, it is characterized in that, described two sample hoses communicate with the inner chamber of collector through separator tube, are that two sample hoses are through the rubber plug central through hole, communicate with the inner chamber of collector through separator tube, filter pulp plug again.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200820123577 CN201321453Y (en) | 2008-11-12 | 2008-11-12 | Two-way device for collecting magnetotactic bacteria |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200820123577 CN201321453Y (en) | 2008-11-12 | 2008-11-12 | Two-way device for collecting magnetotactic bacteria |
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| Publication Number | Publication Date |
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| CN201321453Y true CN201321453Y (en) | 2009-10-07 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 200820123577 Expired - Lifetime CN201321453Y (en) | 2008-11-12 | 2008-11-12 | Two-way device for collecting magnetotactic bacteria |
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| CN (1) | CN201321453Y (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101671631B (en) * | 2009-10-22 | 2012-03-07 | 中南大学 | Magnetic bacteria separometer |
| CN101735937B (en) * | 2008-11-12 | 2012-08-22 | 中国科学院地质与地球物理研究所 | Two-way device for collecting magnetotactic bacteria |
| CN102115746B (en) * | 2009-12-30 | 2013-06-05 | 中国科学院地质与地球物理研究所 | Preparation method of monodisperse magnetic human ferritin |
| CN117247836A (en) * | 2023-11-20 | 2023-12-19 | 山东省国土空间数据和遥感技术研究院(山东省海域动态监视监测中心) | Device and method for collecting different magnetotactic bacteria in ocean in layered manner |
-
2008
- 2008-11-12 CN CN 200820123577 patent/CN201321453Y/en not_active Expired - Lifetime
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101735937B (en) * | 2008-11-12 | 2012-08-22 | 中国科学院地质与地球物理研究所 | Two-way device for collecting magnetotactic bacteria |
| CN101671631B (en) * | 2009-10-22 | 2012-03-07 | 中南大学 | Magnetic bacteria separometer |
| CN102115746B (en) * | 2009-12-30 | 2013-06-05 | 中国科学院地质与地球物理研究所 | Preparation method of monodisperse magnetic human ferritin |
| CN117247836A (en) * | 2023-11-20 | 2023-12-19 | 山东省国土空间数据和遥感技术研究院(山东省海域动态监视监测中心) | Device and method for collecting different magnetotactic bacteria in ocean in layered manner |
| CN117247836B (en) * | 2023-11-20 | 2024-02-02 | 山东省国土空间数据和遥感技术研究院(山东省海域动态监视监测中心) | Device and method for collecting different magnetotactic bacteria in ocean in layered manner |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20091007 Effective date of abandoning: 20081112 |
|
| AV01 | Patent right actively abandoned |
Granted publication date: 20091007 Effective date of abandoning: 20081112 |