CN201062262Y - Biochip hybridization instrument - Google Patents
Biochip hybridization instrument Download PDFInfo
- Publication number
- CN201062262Y CN201062262Y CNU2007201694320U CN200720169432U CN201062262Y CN 201062262 Y CN201062262 Y CN 201062262Y CN U2007201694320 U CNU2007201694320 U CN U2007201694320U CN 200720169432 U CN200720169432 U CN 200720169432U CN 201062262 Y CN201062262 Y CN 201062262Y
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- China
- Prior art keywords
- hybridization
- temperature
- control portion
- biochip
- chamber
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
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- 238000009396 hybridization Methods 0.000 title claims abstract description 70
- 238000000018 DNA microarray Methods 0.000 title claims description 20
- 238000010438 heat treatment Methods 0.000 claims description 14
- 238000001816 cooling Methods 0.000 claims description 12
- 238000004140 cleaning Methods 0.000 claims description 11
- 230000002000 scavenging effect Effects 0.000 claims description 11
- 230000003750 conditioning effect Effects 0.000 claims description 9
- 239000004020 conductor Substances 0.000 claims description 3
- 239000010445 mica Substances 0.000 claims description 3
- 229910052618 mica group Inorganic materials 0.000 claims description 3
- 238000005057 refrigeration Methods 0.000 claims description 3
- 239000004065 semiconductor Substances 0.000 claims description 3
- 230000001105 regulatory effect Effects 0.000 claims description 2
- 238000001035 drying Methods 0.000 abstract description 6
- 239000012530 fluid Substances 0.000 abstract 1
- 230000009514 concussion Effects 0.000 description 9
- 238000000034 method Methods 0.000 description 6
- 238000012360 testing method Methods 0.000 description 5
- 238000013461 design Methods 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 230000001276 controlling effect Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 230000033001 locomotion Effects 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 0 C*=C1*CCC1 Chemical compound C*=C1*CCC1 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 238000005842 biochemical reaction Methods 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000009509 drug development Methods 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000013507 mapping Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002547 new drug Substances 0.000 description 1
- -1 part Proteins 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a biological chip hybridization instrument, which comprises a hybridization cavity, a chip carrying disc, a rotation part, a temperature control part, and a main control part. The chip carrying disc is arranged inside the hybridization cavity, and the chip carrying disc is driven by the rotation part; the rotation control of the rotation part is performed through the main control part, and the temperature inside the hybridization cavity is adjusted through the temperature part. The utility model realizes that the automatic spin drying of hybridization fluid is performed after the hybridization, and the hybridization cavity is automatically cleaned, the operation is simplified and more convenient, and the working time is shortened.
Description
Technical field
The utility model relates to a kind of biochip hybridization device.
Background technology
Biochip technology can carry out integrated to the various biochemical reaction process in life science and the medical science, thereby realizes biologically active substances such as gene, part, antigen are carried out the test and the analysis of efficient quick.Its appearance brings huge innovation even revolution for various fields such as life science, medical science, chemistry, new drug development, biological weapon war, judicial expertise, food and environment supervision.
The research and the application of the aspect such as detection, polymorphism analysis, gene mapping of transgenation have been widely used in based on the analysis of hybridization, hybridization instrument then is to be used for finishing the equipment that biochip is carried out crossover process specially, finishes biochip by it: operations such as prehybridization, hybridization, washing, sealing, enzyme connection, colour developing, color development stopping.Conventional at present biochip hybridization equipment is many, but has following problem usually: 1) each step is independently carried out respectively, and waste liquid need manually siphon away, and is big to operator's dependency, complex operation; 2) temperature has been set to the level adjusting, and it is few to regulate shelves, and optimum control is restricted; 3) but simultaneously hybridization hybrid chip quantity is few, efficient is low; 4) the concussion effect is slow, the hybridization solution long reaction time, and efficient is low.
The utility model content
Technical problem to be solved in the utility model is to provide a kind of biochip hybridization instrument, after biochip is hybridized, can dry hybridization solution automatically.
A kind of biochip hybridization instrument of the present utility model comprises: hybridization chamber, chip load plate, rotating part, temperature control portion and master control portion; The chip load plate is arranged in the hybridization chamber, and this chip load plate drives by rotating part; Described rotating part rotates control by described master control portion, and cavity temperature is regulated by described temperature control portion in described hybridization chamber.
Further, also be provided with on the described hybridization chamber and be used to clean its inner cleaning part, described cleaning part comprises wash tube, scavenging pump and suction pumps, wash tube is arranged in the hybridization chamber, wash tube links to each other with scavenging pump, scavenging pump is injected to scavenging solution in the described hybridization chamber by this wash tube, and described suction pumps links to each other with bottom, described hybridization chamber.
Further, described temperature control portion comprises: temperature conditioning unit and cold and hot air circulation duct, cold and hot air circulation duct is arranged under the described hybridization chamber, described temperature conditioning unit comprises temperature collecting cell, separately controllable a plurality of heating units and cooling module, temperature collecting cell is arranged in the hybridization chamber, and heating unit and cooling module carry out temperature regulation by cold and hot air circulation duct to described hybridization chamber according to the temperature signal of temperature collecting cell collection.
Further, described heating unit is mica heating piece, resistance wire or semi-conductor, and described cooling module is the conductor refrigeration sheet.
Further, also comprise operating portion, this operating portion links to each other with described master control portion, and described master control portion controls described cleaning part, temperature control portion and rotating part by this operating portion.
Further, described operating portion is keyboard and display screen.
Further, described master control portion is PLC programmable logic controller (also can select other similar controlling elements).
The utlity model has following advantage:
1, realized that the hybridization back dries hybridization solution automatically, cleaned the hybridization chamber automatically, simplified operation, and more convenient, shortened experimental period;
2, temperature regulation is a smooth adjustment, and crossover process can be optimized more;
3, concussion is effective, has saved hybridization time;
4, keyboard and display interface close friend are simple to operate;
5, can set works better and test two kinds of operating mode, in time each parts working order of inspection apparatus reduces failure rate;
6, versatility is good, is applicable to multiple biochip hybridization.
Description of drawings
Fig. 1 is the utility model structural representation;
Fig. 2 is the utility model hydrojet ring pipe front view;
Fig. 3 is the vertical view of Fig. 2;
Fig. 4 is a motor control unit block diagram of the present utility model;
Fig. 5 is a system of the present utility model control block diagram.
Embodiment
As shown in Figure 1, the utility model comprises: hybridization chamber 1, rotating part 2, chip load plate 3, operating portion 5, master control portion 6, cleaning part 7 and casing 12.Operating portion 5 is arranged on the top of casing 12, and it forms (not shown go out) by keyboard and display screen.The centre that hybridization chamber 1 is installed in casing 12 on the top, hybridization chamber 1 is provided with chip load plate 3, can place chip cartridges on the chip load plate 3, and chip load plate 3 can be designed as the structure of bearing N chip cartridges, the N optimal selection is 18, and chip cartridges quantity only need change chip load plate 3 when changing area gets final product.Chip load plate 3 drives by rotating part 2, and rotating part 2 rotates and drives 3 rotations of chip load plate, thereby finishes concussion and drying process.Rotating part 2 comprises motor 22 and motor driver 21, and as shown in Figure 4, motor 22 can adopt stepper-motor, so well the concussion of control chip load plate 3 and drying.Can certainly replace with other motors, as long as change control method.Cleaning part 7 comprises wash tube 11, scavenging pump 72 and suction pumps 73, wash tube 11 is arranged in the hybridization chamber 1, wash tube 11 links to each other with scavenging pump 72, scavenging pump 72 is injected to clear water in the hybridization chamber 1 by this wash tube 11, suction pumps 73 links to each other with hybridization 1 bottom, chamber, waste liquid can be discharged hybridization chamber 1.The ring pipe that wash tube 11 is made for materials such as copper, plastics or stainless steels shown in Fig. 2,3, is evenly distributed with spray hole 111 on the excircle of wash tube 11, clean hybridization chamber 1 by spray hole 111 ejection current.
As shown in Figure 1, the utility model also is provided with temperature control portion 8, the cold and hot air circulation duct 82 that this temperature control portion 8 comprises temperature conditioning unit 81 and is provided with below chip carrier 3, cold and hot air circulation duct 82 links to each other with temperature conditioning unit 9, and temperature conditioning unit 9 carries out temperature regulation by 82 pairs of hybridization of cold and hot air circulation duct chamber 1.Temperature conditioning unit 81 comprises temperature collecting cell 811, separately controllable a plurality of heating units and cooling module (not shown go out), and temperature collecting cell is provided with 811 in hybridization chamber 1, is used to gather the temperature in the hybridization chamber 1.Heating unit can adopt mica heating piece, resistance wire, all can produce the element of thermal source semi-conductors etc., cooling module can adopt cooling elements such as conductor refrigeration sheet, and the temperature signal that heating unit and cooling module are gathered according to temperature collecting cell 811 carries out temperature regulation by 82 pairs of hybridization of cold and hot air circulation duct chamber 1.Temperature conditioning unit 81 the other ends link to each other with master control portion 6, and master control portion 6 realizes that by control heating unit and cooling module the temperature of temperature collecting cell 811 collections is consistent with operating portion 5 design temperatures, thereby realize the smooth adjustment of temperature.Temperature collecting cell 811 can adopt temperature sensor.Master control portion 6 can select PLC as master element (also can select other similar controlling elements), master control portion 6 links to each other with the motor driver of operating portion 5 with rotating part 2, can obtain multiple concussion and drying mode by changing the PLC control mode, as shown in Figure 4, master control portion 6 sends pulse control signal to motor driver 21, as direction signal etc., thus the running status of control motor 22, and switch power supply 13 is to motor driver 21 and 6 power supplies of master control portion.As shown in Figure 5, operating portion 5 can be operated by operating portion 5 by 6 control temperature control portions 8 of master control portion, cleaning part 7 and rotating part 2 various controls.
The utility model can be provided with two kinds of operating mode, i.e. normal mode of operation and test pattern.The normal mode of operation operation is as follows: at first select operating mode by operating portion 5, temperature control portion regulates hybridization chamber 1 temperature according to design temperature, master control portion 6 is according to 2 motions of setting pattern controls revolution portion, and rotating part 2 drives 3 motions of chip load plates, finishes concussion and drying.Dry back control part control cleaning part 7 and clean hybridization chamber 1.Test pattern mainly is inspection and the maintenance of carrying out this instrument.Can test each operate portions such as whether temperature control portion 8, rotating part 2 etc. are working properly.
When the utility model used, at first artificial liquid feeding was placed on biochip on the chip load plate 3, by operating portion 5 working parameter is set then, comprises concussion parameter, temperature parameter etc., begins to carry out operations such as biochip hybridization, drying, cleaning then.In this process, can dry hybridization solution automatically after the hybridization, clean hybridization chamber 1 automatically, not only simplify operation, and more convenient, shortened experimental period.In addition,, therefore improved the effect of concussion greatly, saved the time of hybridization owing to can set the concussion parameter.In addition, temperature and operating portion 5 design temperatures that master control portion 6 gathers by C.T. collecting unit 811, control heating unit and cooling module operation, realize that temperature collecting cell 811 collecting temperatures are consistent with operating portion 5 design temperatures, thereby realized the smooth adjustment of temperature, crossover process is optimized more.
Claims (7)
1. a biochip hybridization instrument is characterized in that, comprising: hybridization chamber, chip load plate, rotating part, temperature control portion and master control portion; The chip load plate is arranged in the hybridization chamber, and this chip load plate drives by rotating part; Described rotating part rotates control by described master control portion, and cavity temperature is regulated by described temperature control portion in described hybridization chamber.
2. biochip hybridization instrument as claimed in claim 1, it is characterized in that, also be provided with on the described hybridization chamber and be used to clean its inner cleaning part, described cleaning part comprises wash tube, scavenging pump and suction pumps, wash tube is arranged on hybridization bottom, chamber, wash tube links to each other with scavenging pump, and scavenging pump is injected to scavenging solution in the described hybridization chamber by this wash tube, and described suction pumps links to each other with bottom, described hybridization chamber.
3. biochip hybridization instrument as claimed in claim 1, it is characterized in that, described temperature control portion comprises: temperature conditioning unit and cold and hot air circulation duct, cold and hot air circulation duct is arranged under the described hybridization chamber, described temperature conditioning unit comprises temperature collecting cell, separately controllable a plurality of heating units and cooling module, temperature collecting cell is arranged in the hybridization chamber, and heating unit and cooling module carry out temperature regulation by cold and hot air circulation duct to described hybridization chamber according to the temperature signal of temperature collecting cell collection.
4. biochip hybridization instrument as claimed in claim 3 is characterized in that, described heating unit is mica heating piece, resistance wire or semi-conductor, and described cooling module is the conductor refrigeration sheet.
5. as the arbitrary described biochip hybridization instrument of claim 2 to 4, it is characterized in that, also comprise operating portion, this operating portion links to each other with described master control portion, and described master control portion controls described cleaning part, temperature control portion and rotating part by this operating portion.
6. biochip hybridization instrument as claimed in claim 5 is characterized in that, described operating portion is keyboard and display screen.
7. biochip hybridization instrument as claimed in claim 1 is characterized in that, described master control portion is the PLC programmable logic controller.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2007201694320U CN201062262Y (en) | 2007-06-26 | 2007-06-26 | Biochip hybridization instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2007201694320U CN201062262Y (en) | 2007-06-26 | 2007-06-26 | Biochip hybridization instrument |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201062262Y true CN201062262Y (en) | 2008-05-21 |
Family
ID=39450632
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNU2007201694320U Expired - Lifetime CN201062262Y (en) | 2007-06-26 | 2007-06-26 | Biochip hybridization instrument |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201062262Y (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101085974B (en) * | 2007-06-26 | 2010-06-02 | 北京和利时系统工程有限公司 | Biochip hybridization procedure optimization control method and apparatus employing the method |
| CN102134552A (en) * | 2010-12-31 | 2011-07-27 | 福建泰普生物科学有限公司 | Nucleic acid hybridization method and nucleic acid hybridization instrument utilizing same |
| CN101748046B (en) * | 2009-12-14 | 2012-08-15 | 东南大学 | Full temperature micro volume blending incubation and hybridization instrument |
-
2007
- 2007-06-26 CN CNU2007201694320U patent/CN201062262Y/en not_active Expired - Lifetime
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101085974B (en) * | 2007-06-26 | 2010-06-02 | 北京和利时系统工程有限公司 | Biochip hybridization procedure optimization control method and apparatus employing the method |
| CN101748046B (en) * | 2009-12-14 | 2012-08-15 | 东南大学 | Full temperature micro volume blending incubation and hybridization instrument |
| CN102134552A (en) * | 2010-12-31 | 2011-07-27 | 福建泰普生物科学有限公司 | Nucleic acid hybridization method and nucleic acid hybridization instrument utilizing same |
| CN102134552B (en) * | 2010-12-31 | 2014-05-21 | 福建泰普生物科学有限公司 | Nucleic acid hybridization method and nucleic acid hybridization instrument utilizing same |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C56 | Change in the name or address of the patentee |
Owner name: BEIJING HELISHI SYSTEM ENGINEERING CORPORATION Free format text: FORMER NAME OR ADDRESS: BEIJING HELISHI SYSTEM ENGINEERING CO., LTD. |
|
| CP03 | Change of name, title or address |
Address after: No. 10, city road, building materials, Haidian District, Beijing, Xisanqi: 100096 Patentee after: BEIJING HOLLYSYS Co.,Ltd. Address before: No. 10, city road, building materials, Haidian District, Beijing, Xisanqi: 100096 Patentee before: Beijing Helishi System Engineering Co.,Ltd. |
|
| ASS | Succession or assignment of patent right |
Owner name: BEIJING HELI KANGYUAN MEDICAL TECHNOLOGY CO., LTD. Free format text: FORMER OWNER: BEIJING HOLLYSYS SYSTEM ENGINEERING CO., LTD. Effective date: 20101214 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| C56 | Change in the name or address of the patentee | ||
| CP02 | Change in the address of a patent holder |
Address after: 100176 No. 2 middle Sheng Road, Beijing economic and Technological Development Zone Patentee after: BEIJING HOLLYSYS Co.,Ltd. Address before: 100096, No. 10, building materials Road, Haidian District, Beijing, Xisanqi Patentee before: BEIJING HOLLYSYS Co.,Ltd. |
|
| TR01 | Transfer of patent right |
Effective date of registration: 20101214 Address after: 100176 No. 2 middle Sheng Road, Beijing economic and Technological Development Zone Patentee after: HOLLYSYS AUTOMATION TECHNOLOGIES Ltd. Address before: 100176 No. 2 middle Sheng Road, Beijing economic and Technological Development Zone Patentee before: BEIJING HOLLYSYS Co.,Ltd. |
|
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20080521 |