CN1985861A - Process of preparing Hypericum perforatum piece Chinese medicine tablet - Google Patents
Process of preparing Hypericum perforatum piece Chinese medicine tablet Download PDFInfo
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- CN1985861A CN1985861A CN 200610156355 CN200610156355A CN1985861A CN 1985861 A CN1985861 A CN 1985861A CN 200610156355 CN200610156355 CN 200610156355 CN 200610156355 A CN200610156355 A CN 200610156355A CN 1985861 A CN1985861 A CN 1985861A
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- herba hyperici
- hyperici perforati
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Abstract
The present invention is process of preparing Hypericum perforatum piece and relates to Chinese herbal medicine preparing technology. Available Hypericum perforatum medicine preparing process adopting several kinds of solvent and chemical reagent has complicated technological process, high cost and environment pollution. The present invention adopts a fermentation process, which includes fermentation of Hypericum perforatum meal in activated yeast liquid at 15-50 deg.c for 8-16 days, stoving, crushing and sieving. The fermentation process converts the combined hypericin in Hypericum perforatum into free hypericin, and this is favorable to preservation of hypericin, changes medicine speciality, raises the curative effect and lowers the toxic side effect. In addition, the technological process of the present invention is simple, low in cost and environment friendly.
Description
Technical field
The present invention relates to the field of Chinese medicines, especially the concocting method of medical herbs.
Background technology
Herba Hyperici perforati is Guttiferae (Guttiferae) perennial herb, all herbal medicine, can clear away heart-fire make eye bright, regulating menstruation and activating blood, hemostasia and promoting granulation, detoxicating, relieving inflammation.Modern study is found, its main active hypericin class material (hypericin) not only has critical role on chemical classification, and its medical value is also very high, be used for anti-melancholy, the effect of inhibition nervus centralis except that clinical, still a kind of promising endogenous photosensitizer is used for tumor photochemodiagnosis and treatment.Discover that recently Radix Hyperici Monogyni (Herba Hyperici Monogyni) have significant anti-DNA, RNA viruses effect, is used for the treatment of acquired immune deficiency syndrome (AIDS).The many pharmacy corporations of present China have extracted these type of materials in a large number and have been used for pharmacy and outlet from Herba Hyperici perforati.Utilize the method for hypericum perforatum hypericin mostly to be traditional concocting method at present, use multiple solvent and acid-base class chemical reagent, complex process not only, the cost height also brings adverse factors to environmental conservation.
The Chinese Academy of Agricultural Sciences's Lanzhou herding and the flat seminar of veterinary drug institute beam sword result of study show that hyperforine all has very strong antivirus action in vivo and in vitro to bird flu.Hypericin (HY) and pseudohypericin (PHY) are the focuses of hyperforine applied research to the antivirus action of multiple retrovirus (comprising HIV (human immunodeficiency virus)), enveloped virus.Fermentation method is one of method of Chinese medicine processing, by means of action of microorganisms, changes the original property of medicine of Chinese medicine, improves curative effect, reduces toxic and side effects, enlarges indication.As Massa Medicata Fermentata, Semen Sojae Preparatum, Pinellia leaven etc. all are the medicines that form by fermentation.Microorganism can produce various enzymes in growth course, these enzymes can decompose the toxic and side effects that reduces medicine with the composition decomposition and inversion of medicine for new active component or with toxic component.Fermentation method is applied to the Herba Hyperici perforati process of preparing Chinese medicine and makes the prepared slices of Chinese crude drugs, does not still have bibliographical information at present.
Summary of the invention
Purpose of the present invention is utilized the preparation method of the Herba Hyperici perforati prepared slices of Chinese crude drugs of the fermentation method process of preparing Chinese medicine for providing a kind of method simple.
The technical scheme that realizes above-mentioned purpose is as follows.
The preparation method of the Herba Hyperici perforati prepared slices of Chinese crude drugs:
(1) get edible rice flour 80-110g, add water 1000ml, add dry yeast 10g, insulation is 2 hours in 30-40 ℃ of water-bath, obtains activated yeast mixture, and is standby;
(2) get Herba Hyperici perforati coarse powder 90-110g, add activated yeast mixture 20-200ml, mix thoroughly, 15-50 ℃ heat-preservation fermentation 8-16 days;
(3) with the Herba Hyperici perforati coarse powder of fermentation 30-80 ℃ of oven dry, pulverize, cross the 50-200 mesh sieve, get product.
Improved preparation method is as follows:
(1) get edible rice flour 90-105g, add water 1000ml, add dry yeast 10g, insulation is 2 hours in 35-37 ℃ of water-bath, obtains activated yeast mixture, and is standby;
(2) get Herba Hyperici perforati coarse powder 95-105g, add activated yeast mixture 50-150ml, mix thoroughly, at 20-40 ℃ of heat-preservation fermentation 8-16 days;
(3) with the Herba Hyperici perforati coarse powder of fermentation 60-80 ℃ of oven dry, pulverize, cross the 100-200 mesh sieve, get product.
Preferred manufacturing procedure is as follows:
(1) get edible rice flour 100g, add water 1000ml, add dry yeast 10g, insulation is 2 hours in 37 ℃ of water-baths, obtains activated yeast mixture, and is standby;
(2) get Herba Hyperici perforati coarse powder 100g, add activated yeast mixture 100ml, mix thoroughly, at 37 ℃ of heat-preservation fermentation 8-16 days;
(3) with the Herba Hyperici perforati coarse powder of fermentation 80 ℃ of oven dry, pulverize, cross 200 mesh sieves, get product.
The preparation method of the another kind of Herba Hyperici perforati prepared slices of Chinese crude drugs at the above-mentioned hypericin crude extract 1-50g that in the Herba Hyperici perforati powder 100g that fermentation makes, adds through 30-50 ℃ of oven dry, pulverize the Herba Hyperici perforati prepared slices of Chinese crude drugs that sieve and promptly get the high concentration hypericin.
The preparation method of another Herba Hyperici perforati prepared slices of Chinese crude drugs is fermented through said method for add Radix Hyperici Monogyni (Herba Hyperici Monogyni) crude extract 1-50g in Herba Hyperici perforati powder 100g, through 30-50 ℃ of oven dry, pulverizes the Herba Hyperici perforati prepared slices of Chinese crude drugs that sieve and promptly get the high concentration hypericin.
Use the total content and the free amount of high effective liquid chromatography for measuring hypericin by the Herba Hyperici perforati of the technique scheme process of preparing Chinese medicine.
The mensuration of hypericin in the sample is got Herba Hyperici perforati (crossing 60 mesh sieves) 200mg, places flask, adds the 150ml methanol eddy and extracts 1h, filtered while hot, filtrate for later use.
The mensuration of free hypericin: the accurate sample liquid 5ml that draws, place conical flask, water bath method organic solution, add the 5ml distilled water, shake up, with 15ml chloroform single extraction, reclaim or the evaporate to dryness chloroform, add dissolving of 0.8% magnesium acetate methanol solution and 10ml volumetric flask standardize solution, measure peak area, the percentage composition of substitution regression equation calculation as a result in the 588nm place.
The mensuration of total hypericin: the accurate sample liquid 2ml that draws, place conical flask, evaporate to dryness organic solution is measured peak area in the 588nm place, calculates percentage composition.
Two primary yeasts fermentation back is to the comparison of hypericin influence
Adopt bakery yeast, distillery yeast respectively after making Herba Hyperici perforati fermentation 1d, 2d, 4d, 8d, 16d under 37 ℃ of conditions, measure the content of its total hypericin and free type hypericin, the results are shown in Table 1, table 2.
Table 1 Herba Hyperici perforati and the prepared slices of Chinese crude drugs (distillery yeast fermentation product) hypericin content and relative value
| Hypericin content | Herba Hyperici perforati is given birth to product | Through distillery yeast fermentation product | ||||
| 1d | 2d | 4d | 8d | 16d | ||
| Free type amount | 0.353(100) | 0.727(206) | 0.841(238) | 1.073(304) | 1.462(414) | 2.098(594) |
| Total amount | 4.184(100) | 4.108(98.2) | 4.135(98.8) | 4.169(99.6) | 4.124(98.6) | 4.095(97.8) |
Table 2 Herba Hyperici perforati and the prepared slices of Chinese crude drugs (bakery yeast fermentation product) hypericin content and relative value
| Hypericin content | Herba Hyperici perforati is given birth to product | Through bakery yeast fermentation product | ||||
| 1d | 2d | 4d | 8d | 16d | ||
| Free type amount | 0.353(100) | 0.873(247) | 0.981(278) | 1.102(312) | 1.533(434) | 2.288(648) |
| Total amount | 4.184(100) | 4.135(98.8) | 4.123(98.5) | 4.084(97.6) | 4.137(98.9) | 4.110(98.2) |
Herba Hyperici perforati is after 37 ℃ of distillery yeasts, bakery yeast fermented respectively 16 days, and total hypericin content slightly reduces 97.8%, 98.2% of the product of making a living respectively.Compare with document, traditional concocting method all generally makes the total hypericin content of Herba Hyperici perforati obviously reduce, and illustrates that fermentation method helps preserving the total hypericin of Herba Hyperici perforati, and fermentation method is better than traditional concocting method.Free type hypericin is the main effective ingredient of antiviral etc., a little less than the side effect extremely, after 37 ℃ of distillery yeasts, bakery yeast fermented respectively 16 days, conjunction type hypericin content reduces, 52%, 48% of the product of making a living respectively, free type hypericin content increases greatly, and the 5-6 of the product of making a living respectively doubly.Illustrate that fermentation method can make Herba Hyperici perforati conjunction type hypericin be converted into free type hypericin.
Fermentation is better than traditional concocting method reason in the prepared slices of Chinese crude drugs:
1, is opened by the fermenting plant cell wall, after taking, wraps in the interior hypericin of cell wall and easily be absorbed and used.
2, by fermentation, can make class materials such as hypericin in the Herba Hyperici perforati, pseudohypericin, hyperin dissociate out, become free type, strengthen the hypericin action effect from conjunction type.
Comprehensive above-mentioned test situation, the present invention has following beneficial effect:
(1) fermentation method helps preserving the total amount of the hypericin in the Herba Hyperici perforati, so fermentation method is better than traditional concocting method.
(2) fermentation method helps the conjunction type hypericin in the Herba Hyperici perforati is converted into free type hypericin, so the Herba Hyperici perforati after the fermentation has reduced side effect, has improved curative effect.
(3) the fermentation method processing procedure is simple, does not use chemical agent, has not only reduced cost, also is of value to environmental conservation.
The specific embodiment
Following embodiment now is provided, technical scheme of the present invention is further elaborated.
1. the preparation of activated yeast mixture:
(1) get edible rice flour 80g, add water 1000ml, add dry yeast 10g, insulation is 2 hours in 40 ℃ of water-baths.
(2) get edible rice flour 110g, add water 1000ml, add dry yeast 10g, insulation is 2 hours in 30 ℃ of water-baths.
(3) get edible rice flour 100g, add water 1000ml, add dry yeast 10g, insulation is 2 hours in 37 ℃ of water-baths.
2. the preparation of the fermentation of Herba Hyperici perforati and decoction pieces:
(1) gets Herba Hyperici perforati coarse powder 90g, add activated yeast mixture 20ml, mix thoroughly, 50 ℃ of heat-preservation fermentations 8 days; 35 ℃ of oven dry, pulverize, cross 50 mesh sieves promptly.
(2) get Herba Hyperici perforati coarse powder 110g, add activated yeast mixture 200ml, mix thoroughly, 15 ℃ of heat-preservation fermentations 16 days; 80 ℃ of oven dry, pulverize, cross 100 mesh sieves promptly.
(3) get Herba Hyperici perforati coarse powder 100g, add activated yeast mixture 100ml, mix thoroughly, 37 ℃ of heat-preservation fermentations 8 days; 80 ℃ of oven dry, pulverize, cross 200 mesh sieves promptly.
3. the preparation of the Herba Hyperici perforati prepared slices of Chinese crude drugs of high concentration hypericin:
(1) gets the Herba Hyperici perforati powder 100g that above-mentioned fermentation makes, add hypericin crude extract 1g,, pulverize, cross 200 mesh sieves promptly through 30 ℃ of oven dry.
(2) get the Herba Hyperici perforati powder 100g that above-mentioned fermentation makes, add hypericin crude extract 25g,, pulverize, cross 200 mesh sieves promptly through 40 ℃ of oven dry.
(3) get the Herba Hyperici perforati powder 100g that above-mentioned fermentation makes, add hypericin crude extract 50g,, pulverize, cross 200 mesh sieves promptly through 50 ℃ of oven dry.
4. the preparation of the Herba Hyperici perforati prepared slices of Chinese crude drugs of another high concentration hypericin:
(1) adds hypericin crude extract 1g among the Herba Hyperici perforati powder 100g,,, pulverize, cross 200 mesh sieves promptly through 30 ℃ of oven dry through the said method fermentation.
(2) add hypericin crude extract 25g among the Herba Hyperici perforati powder 100g and ferment,, pulverize, cross 200 mesh sieves promptly through 40 ℃ of oven dry through said method.
(3) add hypericin crude extract 50g among the Herba Hyperici perforati powder 100g and ferment,, pulverize, cross 200 mesh sieves promptly through 50 ℃ of oven dry through said method.
Claims (5)
1. the preparation method of the Herba Hyperici perforati prepared slices of Chinese crude drugs is characterized by:
(1) get edible rice flour 80-110g, add water 1000ml, add dry yeast 10g, insulation is 2 hours in 30-40 ℃ of water-bath, obtains activated yeast mixture, and is standby;
(2) get Herba Hyperici perforati coarse powder 90-110g, add activated yeast mixture 20-200ml, mix thoroughly, 15-50 ℃ heat-preservation fermentation 8-16 days;
(3) with the Herba Hyperici perforati coarse powder of fermentation 30-80 ℃ of oven dry, pulverize, cross the 50-200 mesh sieve, both finished product.
2. the preparation method of the Herba Hyperici perforati prepared slices of Chinese crude drugs according to claim 1, it is as follows to it is characterized by improved preparation method:
(1) get edible rice flour 90-105g, add water 1000ml, add dry yeast 10g, insulation is 2 hours in 35-37 ℃ of water-bath, obtains activated yeast mixture, and is standby;
(2) get Herba Hyperici perforati coarse powder 95-105g, add activated yeast mixture 50-150ml, mix thoroughly, at 20-40 ℃ of heat-preservation fermentation 8-16 days;
(3) with the Herba Hyperici perforati coarse powder of fermentation 60-80 ℃ of oven dry, pulverize, cross the 100-200 mesh sieve, both finished product.
3. the preparation method of the Herba Hyperici perforati prepared slices of Chinese crude drugs according to claim 1 and 2, it is as follows to it is characterized by preferred manufacturing procedure:
(1) get edible rice flour 100g, add water 1000ml, add dry yeast 10g, insulation is 2 hours in 37 ℃ of water-baths, obtains activated yeast mixture, and is standby;
(2) get Herba Hyperici perforati coarse powder 100g, add activated yeast mixture 100ml, mix thoroughly, at 37 ℃ of heat-preservation fermentation 8-16 days;
(3) with the Herba Hyperici perforati coarse powder of fermentation 80 ℃ of oven dry, pulverize, cross 200 mesh sieves, both finished product.
4. according to the preparation method of the claim 1 or the 2 or 3 described Herba Hyperici perforati prepared slices of Chinese crude drugs, it is as follows to it is characterized by the preparation method that contains the high concentration hypericin: at the above-mentioned hypericin crude extract 1-50g that adds in the Herba Hyperici perforati powder 100g that fermentation makes, through 30-50 ℃ of oven dry, pulverize, cross 200 mesh sieves, promptly get the Herba Hyperici perforati prepared slices of Chinese crude drugs of high concentration hypericin.
5. according to the preparation method of the prepared slices of Chinese crude drugs of claim 1 or 2 or 3 described Herba Hyperici perforatis, it is as follows to it is characterized by another preparation method that contains the high concentration hypericin: add hypericin crude extract 1-50g in Herba Hyperici perforati powder 100g, ferment through said method, through 30-50 ℃ of oven dry, pulverize the Herba Hyperici perforati prepared slices of Chinese crude drugs that sieve and promptly get the high concentration hypericin.
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|---|---|---|---|
| CN 200610156355 CN1985861A (en) | 2006-12-31 | 2006-12-31 | Process of preparing Hypericum perforatum piece Chinese medicine tablet |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103859168A (en) * | 2014-04-03 | 2014-06-18 | 常熟理工学院 | Avian influenza resistant feed additive for large-scale ecological chicken breeding and preparation and use methods thereof |
| CN105146537A (en) * | 2015-08-18 | 2015-12-16 | 四川中庸药业有限公司 | Healthy kitchen medicinal slice preparation method |
-
2006
- 2006-12-31 CN CN 200610156355 patent/CN1985861A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103859168A (en) * | 2014-04-03 | 2014-06-18 | 常熟理工学院 | Avian influenza resistant feed additive for large-scale ecological chicken breeding and preparation and use methods thereof |
| CN105146537A (en) * | 2015-08-18 | 2015-12-16 | 四川中庸药业有限公司 | Healthy kitchen medicinal slice preparation method |
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