CN1973620A - Industrial abacterial housefly raising method - Google Patents
Industrial abacterial housefly raising method Download PDFInfo
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- CN1973620A CN1973620A CN 200610124226 CN200610124226A CN1973620A CN 1973620 A CN1973620 A CN 1973620A CN 200610124226 CN200610124226 CN 200610124226 CN 200610124226 A CN200610124226 A CN 200610124226A CN 1973620 A CN1973620 A CN 1973620A
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Abstract
The industrial abacterial housefly raising method includes the following steps: 1. preparing abacterial housefly egg through sterilizing the housefly egg with disinfectant comprising 0.5 % concentration sodium hypochlorite solution and 1.5 % concentration sodium hydroxide solution for 5-30 min; 2. culturing housefly larva in sterilized culture medium; and 3. culturing housefly through adding abacterial milk powder and water and lighting with natural light or artificial light for at least 3 hr each day. The method can obtain abacterial housefly.
Description
Technical field
The present invention relates to the industrialization raising method of housefly.
Background technology
Housefly (Musca domestica) belongs to Insecta, diptera, Aristocera, tame Nuscidae, is a kind of that the most of area of China is the most common, quantity is maximum.Housefly is good animal protein feed, has advantages such as fertility is strong, life cycle short, be easy to intensive production, feed resource is abundant, is classified as in the world first of the novel protein resource.
The crude protein content of fly maggot and fresh fish, fish meal and meat and bone meal are close or slightly high.Fly maggot contains the needed several amino acids of animal, and its each amino acid content all is higher than fish meal, the reference value 40% that essential amino acid all proposes above international Food and Agriculture Organization/World Health Organization (FAO/WHO).Macroelement and micronutrient levels are also very abundant, have detected more than 17 kinds, and the zinc content that wherein is described as " flower of life " is 40 times of royal jelly liquid; Vitamin content is also very abundant, and is the highest with vitamin B group content, and B1, B2 surpass 15 times and 1800 times in milk respectively.Nasa in addition with common house-fly grub as astronaut's space food.Housefly is the high-quality protein source that can substitute imported fish meal fully.
Housefly also has various abundant bioactivators.The contained chitin of housefly maggot skin and pupal cell is higher than shrimp, crab 10～20 times, can further be processed into shitosan and novel nourishing-tunicin etc., in scientific research with medically be with a wide range of applications.In addition, under certain micro-organisms and specific inductive condition effect, fly maggot can be secreted and produce multiple antibiotic, antiviral polypeptides material.These antibacterial peptides not only have wide spectrum antibacterium ability, and fungi, virus are also had effect.In recent years, because the abuse of medicine, problem such as medicament residue and bacterial drug resistance is day by day serious, thereby caused the concern of people to food and environment, more and more countries begins to appeal the forbidding antibiotic, and antibacterial peptide is active and be different from traditional antibiotic special role mechanism because of its unique biological, has caused that people study interest greatly, becomes one of the focus in molecular biology and biochemical research field.
But traditional housefly feeding manner utilizes the ight soil of fowl domestic animals to raise fly maggot, and the output of its product and quality are very unstable, and have various etiologies.If be applied to feed and medical industry, housefly must the harmless raising of industrialization.In order to reach harmless raising, plant fly and should satisfy aseptic raising.CN1213497 and CN1360824 have proposed the raising method of some aseptic houseflies, the important measures of these methods are that housefly pupal cell or the larva of raising carried out disinfection, but according to Entomological Physiology knowledge, may there be many microorganisms in insect pupa of in natural open environment, living and the enteron aisle of its larva, fail effectively to eliminate these microorganisms by body surface sterilization pupal cell or its larva body, thereby be difficult to reach the effect that housefly is raised asepticize.
Summary of the invention
The objective of the invention is a kind of industrial abacterial raising method that can effectively realize the housefly of housefly asepticize.
Housefly industrial abacterial raising method proposed by the invention comprises the steps:
(1) preparation of the aseptic ovum of housefly: under aseptic condition, the ovum that adult housefly is given birth to was sterilized 5-30 minute with the sodium hydroxide of disinfectant 0.5% clorox and 1.5%;
(2) common house-fly grub is cultivated: will place the medium of sterilization to cultivate into larva through the tame fly blow of said method sterilization, described medium be pressed wheat bran by wheat bran and water: water=(1-5): (9-5) mix, shaking while hot behind autoclaving looses forms;
(3) cultivation of adult: larva in above-mentioned medium under gnotobasis through being grown to into fly pupa time, in medium, add aseptic milk powder after becoming fly, irregularly add sterile water and every day available light or artificial lighting at least 3 hours.
In above-mentioned steps (2), the cultivation temperature of larva generally is controlled at 25-32 ℃.
In step (3), can irregularly in medium, add a small amount of sterile water by the method for dipping in water with aseptic sponge or cotton.
Adopt the selected disinfectant of the present invention that man fly blow is carried out disinfection, can kill the microorganism on ovum surface effectively, the incubation rate of ovum is also very high, generally is higher than 90%, and makes its larva that hatches reach germ-free condition.And in medium, add aseptic milk powder, irregularly add sterile water and every day available light or artificial lighting at least 3 hours, then be the three big conditions that aseptic one-tenth fly is raised, can promote into sexual maturity and the normally mating of fly.
With each the worm attitude of housefly that obtains in the above-mentioned feeding process, draw culture medium flat plate (nutrient agar panel, PDA lithographic plate) and add simultaneously in liquid nutritional broth bouillon and the PDA medium and detect, the result shows that detecting flat board upward and in the liquid nutrient medium does not see growth of microorganism.As seen the ovum given birth to of common house-fly grub, pupa, adult and the adult of raising by method of the present invention all is aseptic, reaches the effect that housefly is raised asepticize fully.The inventive method science is feasible, makes the industrial abacterial raising of housefly become reality.
Embodiment
Embodiment
The step of housefly industrial abacterial raising method is as follows:
(1) preparation of the aseptic ovum of housefly: under aseptic condition, the ovum that adult housefly is given birth to sterile water wash for several times after, with the sodium hydroxide sterilization of disinfectant 0.5% clorox and 1.5% 5-30 minute, clean with clear water then, remove disinfectant.It is aseptic that this disinfects the ovum that can guarantee housefly, and ovum can normally be hatched.
(2) preparation of aseptic culture medium: with wheat bran and water according to certain ratio (wheat bran: water=3: 7) mix conventional autoclaving (121 ℃ 30 minutes).After the sterilization, shake diffusing medium while hot.
(3) common house-fly grub is cultivated: the tame fly blow of said method sterilization is added in the medium of sterilization and cultivate.According to the food situation of getting of larva, irregularly replenish aseptic culture medium.Cultivation temperature is 28 ℃.
(4) cultivation of adult: under aseptic condition, larva is through being grown to normal one-tenth fly pupa time, the raising condition of aseptic one-tenth fly must satisfy three big conditions simultaneously: the aseptic milk powder of adding in medium, the adding sterile water and every day available light or artificial lighting at least 3 hours.Aseptic milk powder is as the additional albumen that becomes fly, and is very important to the normal development that becomes fly.Irregularly dip in a small amount of sterile water with aseptic sponge or cotton and add in the medium, can replenish into the moisture that fly needs.Sexual maturity that the available light of supply every day or artificial lighting can promote into fly and normal mating.
Claims (3)
1. the industrial abacterial raising method of a housefly is characterized in that comprising the steps:
(1) preparation of the aseptic ovum of housefly: under aseptic condition, the ovum that adult housefly is given birth to was sterilized 5-30 minute with the sodium hydroxide of disinfectant 0.5% clorox and 1.5%;
(2) common house-fly grub is cultivated: will place the medium of sterilization to cultivate into larva through the tame fly blow of said method sterilization, described medium be pressed wheat bran by wheat bran and water: water=(1-5): (9-5) mix, shaking while hot behind autoclaving looses forms;
(3) cultivation of adult: larva in above-mentioned medium under gnotobasis through being grown to into fly pupa time, in medium, add aseptic milk powder after becoming fly, irregularly add sterile water and every day available light or artificial lighting at least 3 hours.
2. the industrial abacterial raising method of housefly according to claim 1 is characterized in that the cultivation temperature of larva in the step (2) is 25-32 ℃.
3. the industrial abacterial raising method of housefly according to claim 1 is characterized in that step (3) adds sterile water in medium method is: irregularly dip in a small amount of sterile water with aseptic sponge or cotton and add in the medium.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610124226 CN1973620A (en) | 2006-12-15 | 2006-12-15 | Industrial abacterial housefly raising method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN 200610124226 CN1973620A (en) | 2006-12-15 | 2006-12-15 | Industrial abacterial housefly raising method |
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| CN1973620A true CN1973620A (en) | 2007-06-06 |
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| CN 200610124226 Pending CN1973620A (en) | 2006-12-15 | 2006-12-15 | Industrial abacterial housefly raising method |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101999543A (en) * | 2010-12-03 | 2011-04-06 | 广东省昆虫研究所 | House fly larva feed |
| CN102550487A (en) * | 2011-01-21 | 2012-07-11 | 中国人民解放军第四五四医院 | Production method of aseptic maggots |
| CN105104308A (en) * | 2015-07-22 | 2015-12-02 | 青岛农业大学 | Free-living marine nematode liquid culture solution and application thereof |
| TWI623261B (en) * | 2014-12-03 | 2018-05-11 | National Pingtung Univ Of Science And Technology | Method for producing sterile fly eggs |
| CN113243339A (en) * | 2021-04-28 | 2021-08-13 | 华南师范大学 | Simple, convenient and efficient preparation method of aseptic fruit flies |
| CN113261536A (en) * | 2021-05-19 | 2021-08-17 | 重庆市中药研究院 | Sterilization treatment method for hepialus armoricanus eggs |
-
2006
- 2006-12-15 CN CN 200610124226 patent/CN1973620A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101999543A (en) * | 2010-12-03 | 2011-04-06 | 广东省昆虫研究所 | House fly larva feed |
| CN101999543B (en) * | 2010-12-03 | 2013-04-24 | 广东省昆虫研究所 | House fly larva feed |
| CN102550487A (en) * | 2011-01-21 | 2012-07-11 | 中国人民解放军第四五四医院 | Production method of aseptic maggots |
| TWI623261B (en) * | 2014-12-03 | 2018-05-11 | National Pingtung Univ Of Science And Technology | Method for producing sterile fly eggs |
| CN105104308A (en) * | 2015-07-22 | 2015-12-02 | 青岛农业大学 | Free-living marine nematode liquid culture solution and application thereof |
| CN105104308B (en) * | 2015-07-22 | 2016-03-09 | 青岛农业大学 | A kind of Free-living amoeba liquid medium and application thereof |
| CN113243339A (en) * | 2021-04-28 | 2021-08-13 | 华南师范大学 | Simple, convenient and efficient preparation method of aseptic fruit flies |
| CN113261536A (en) * | 2021-05-19 | 2021-08-17 | 重庆市中药研究院 | Sterilization treatment method for hepialus armoricanus eggs |
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Open date: 20070606 |