JP2000143521A - Biological material for animal, feed and chemical for plant - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a biological material for an animal capable of preventing the disease by activating the immunological functions originally possessed by various kinds of livestock and poultry, manifesting antistress activities, and effective for growing the healthy and safe livestock and poultry by including a metabolite or product of a specific peptide or the like as an active ingredient. SOLUTION: This biological material for an animal contains a metabolite or a product of a peptide or the like obtained by carrying out the mixed culture of Bacillus thuringiensis with Bacillus pumilus as an active ingredient. For example, the objective material is obtained by using the sludge for treating waste water, giving priority to both of the Bacillus thuringiensis and the Bacillus pumilus, leaf mold including comparatively high density thereof, or the like as seed bacteria, inoculating the seed bacteria with nutrients in a culturing vessel, repeating proliferation steps of spore-making and degradation by aeration and addition of nutrient to make the concentration of the bacteria high, and drying the obtained sludge.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a biological material as an animal drug and a feed to which the biological material is added.
The present invention relates to a biological material having a remarkable effect on immunostimulation, prevention of infectious diseases, and anti-stress, and an animal feed to which the biological material is added at an appropriate ratio. In addition, the present invention relates to an agent which is effective in preventing, curing, and controlling pests of bacterial diseases of plants.

[0002]

2. Description of the Related Art In recent years, in poultry farms, pig farms, barns, etc., these livestock and poultry have been pushed into extremely limited living spaces, and breeding that meets only the purpose of production has not been pursued. Produced by the method. Therefore, these livestock and poultry are suffering from various stresses caused by their living environment and breeding, resulting in a decrease in their health status and an increase in the frequency of seeing outbreaks of infectious diseases. Further, there are pathogenic bacteria and harmful and illnesses caused by the compound feed raw materials, and immunity reduction and side-effect disorders caused by chemicals added for the purpose of preventing them. In addition, various sulfa drugs and antibiotics are usually administered for infectious diseases, and resistant bacteria to these drugs have emerged. Ingested into the body, there are fears of various adverse effects. As described above, a decrease in the productivity of livestock and poultry (disease risk) and a decrease in the quality due to chemical substances and antibiotics cause a great loss, and there is a strong demand for an epidemic prevention measure that does not depend on chemotherapy. On the other hand, in plant cultivation,
The problem of environmental pollution and food pollution due to increased consumption of pesticides has become a major social problem. And the movement of pesticide-free cultivation and organic cultivation is also becoming active, but it is impossible to switch over overnight. Therefore, the emergence of a botanical agent that does not cause environmental pollution or food pollution is desired.

[0003]

SUMMARY OF THE INVENTION Accordingly, the present inventors
An object of the present invention is to activate the immune function inherent in various livestock and poultry to prevent diseases and to provide substances for raising healthy and safe livestock and poultry. Further, the present invention provides and uses a safe plant drug which does not cause environmental pollution and food pollution.

[0004]

DISCLOSURE OF THE INVENTION The present invention has been made to solve the above-mentioned problems, and it is an object of the present invention to provide Bacillus thuringiensis.
thuringensis and Bacillus pum
biological materials containing metabolites and products such as peptides by mixed culture with priority of
When purified metabolites and products such as peptides (hereinafter referred to as metabolites) are added to feed and administered to pigs and chickens, these livestock and poultry naturally have various immune functions. It has been confirmed that is activated and the stress is alleviated, thereby preventing infection by viruses and bacteria. Thus, the present invention has been completed.

[0005] This biological material is used as a seed for sewage treatment sludge in which both Bacillus bacteria, especially Bacillus thuringiensis and Bacillus pumilus, have become dominant, and humus, etc., which contain these substances at a relatively high density. In addition, the bacteria are inoculated in a culture tank, and a growth process called sporulation and decomposition is repeated by aeration and nutrient addition to densify Bacillus thuringiensis and Bacillus pumilus, and the obtained sludge is dried to obtain. Alternatively, the fermented sludge obtained by further fermenting the sludge to decompose organic substances such as cell membranes of bacterial cells and carcasses of protozoa and further densifying the Bacillus is obtained.

[0006] During the growth step or the fermentation treatment, particularly during the fermentation treatment, Bacillus thuringiensis or Bacillus
Pumilus is dominant due to its short life time, and most of Bacillus bacteria are occupied by them. The concentration of Bacillus thuringiensis or Bacillus pumilus in the biological material is on the order of 10 ¹⁰ to 10 ¹² cells / g. Small amounts of other Bacillus bacteria such as Bacillus subtilis and yeast may be present in these sludges. The point is that Bacillus thuringiensis and Bacillus pumilus should dominate. In addition, Bacillus thuringiensis has a cell size of 1.2 μmφ or more and can use starch to degrade starch, saccharides and some strains, but cannot degrade Goodmeat, whereas Bacillus pumilus has a cell size of 1.1 μmφ.
In the following, cooked meat casein is decomposed and utilized and does not degrade starch, so that it is uniquely identified from other Bacillus bacteria.

That is, the biological material referred to in the present invention is a fermentation intermediate or a fermentation product containing metabolites and products such as peptides obtained as a result of mixed culture of Bacillus thuringiensis and Bacillus pumilus as described above. It refers to a mixture composed of residual substances, Bacillus spores, and the like. Here, the metabolite refers to a substance secreted by the activity or sporulation of the bacterial cell, particularly sporulation, and includes a cyclic peptide having an antibiotic action and some polypeptides. The product is
It includes decomposition products of nutrients and reaction products of the decomposition products, such as ammonia, hydrogen sulfide, amino acids, and polypeptides produced by the reaction of amino acids with substances having a carbosyl group. Then, metabolites and products such as polypeptides and cyclic peptides contained in the biological material are eluted in a solvent such as dilute ethanol or water, and only these can be recovered and used as drugs. Further, the fermentation mixture also includes fermentation auxiliary materials such as rice husk charcoal and digests thereof. Its characteristic is that it contains extremely high concentrations of metabolites. In other words, Bacillus thuringiensis and Bacillus pumilus repeatedly preferentially repeat the growth cycle of spore germination, proliferation, demolition, and sporulation, and increase the concentration of metabolites secreted by their activity and sporulation. It is.

It is known that Bacillus pumilus produces antibacterial substances, antibiotics, and physiologically active substances (Japanese Patent Publication No. 57-6913, Japanese Patent Publication No. 61-12914, Japanese Patent No.
6479). It is also known that Bacillus thuringiensis produces a nematode toxin (Japanese Patent Application Laid-Open No. 64-67).
192). However, the mixed culture of Bacillus thuringiensis and Bacillus pumilus, and as a result of the mixed culture, the production of high concentrations of substances that are highly effective in immunostimulation for animals, prevention of infection, and anti-stress, and furthermore metabolites It is not known to act synergistically. It is also not known that Bacillus thuringiensis produces immunostimulants.

In general, Bacillus decomposes nutrients such as proteins, carbohydrates, lipids, urea and uric acid under aerobic or facultative anaerobic conditions and moderate temperature and humidity in accordance with the bacterial species. It grows and forms endospores with the lack of growth conditions and eventually breaks down into spores. Spores are resistant to adverse environmental conditions,
It germinates with the emergence of growth conditions while maintaining life, and the same process is repeated to increase the bacterial density (spore density). When the bacteria metabolize and grow, they produce metabolites.

Among them, Bacillus thuringiensis is particularly excellent in decomposing performance of polysaccharide, and Bacillus pumilus is excellent in decomposing performance of monosaccharide and protein. Therefore, when the two are mixed and cultured, nutrient degradation is shared, and the two grow together with each other. When an oligotrophic state is caused, the bacillus state and the Bacillus in the filamentous body die, but the remaining spores of Bacillus thuringiensis and Bacillus pumilus maintain their lives, and the Bacillus dead cell wall and cytoplasm And the polysaccharides contained in the spore shells are Bacillus
Turingensis decomposes and assimilates, and Bacillus pumilus decomposes and assimilates monosaccharides and proteins obtained by the decomposition, and Bacillus thuringiensis and Bacillus pumilus mutually promote and prioritize each other. As a result of repeating the growth process, these Bacillus thuringiensis and Bacillus pumilus cells and spore densities were 10 ¹⁰ to 10 ¹² cells / g.
Although as many as, poor nutritional status (digestive fermentation, fermentation under oligotrophic) when to appear repeatedly, the density decreases to the natural distribution and 10 ² to 10 ⁴ / g as dormant spores ing. However, the metabolites and the like are further concentrated even in the oligotrophic growth process.

From the biological material, an active ingredient such as a metabolite is eluted (extracted) using a solvent, and this is left in a liquid state or further dried to be powdered to obtain an animal drug or a plant drug. Most preferably, the solvent is water or dilute ethanol. This drug has a remarkable effect on immunostimulation, infection prevention and anti-stress effect of bred animals, prevention of bacterial disease of plants, healing and pest control. The remainder of the active ingredient eluted from the biological material is composed of fermentation auxiliary materials such as rice hulls used for fermentation, digested products thereof, Bacillus spores, and the like, and is therefore preferably used as a soil conditioner. In addition, Bacillus thuringiensis and Bacillus pumilus do not germinate and activate in the animal body, and have no harmful effect on animals (EPA report, WHO report, etc.). Experiments have also confirmed that Bacillus spores only pass through the animal in that state.

The animals to be used in the present invention include domestic animals such as pigs and cows, pets such as dogs and cats, poultry such as chickens, quails and ducks, birds for pets, and animals raised in zoos. Is included. Infectious diseases include pork erysipelas, edema, anthrax in cattle and pigs, tuberculosis in cattle, bacterial diseases such as chicken chick diarrhea, swine cholera, Japanese encephalitis in pigs, Ibaraki disease in cattle, bovine fever, It refers to all viral, bacterial, fungal and parasitic infections, as well as viral diseases such as Newcastle disease in chickens. still,
The feed of the present invention is not particularly limited, and any feed may be used as long as it is used according to the type of livestock, poultry, and other animals.

[0013] These infectious diseases are of course caused by pathogenic bacteria and viruses. However, at present, breeding in overcrowded pigs and chickens in narrow gauges in a dense and poor environment is a normal condition. Pathogen resistance and reduced immunity are also major factors. For example, in pigs, stress causes diarrhea or bites in the tail, and in chickens, feathers and diarrhea. Salmonella contamination is also serious. Therefore, under these circumstances, pig-raising and poultry raising with drugs are carried out for these measures. In contrast, the biological material of the present invention has an anti-stress effect,
Elimination or reduction of these stress phenomena,
The risk of raising pigs and poultry is greatly reduced. Furthermore, the stench generated from the barn and the poultry house causes odor pollution to the neighborhood, causing serious social problems in various places. When the biological material of the present invention is administered, the stench generated in the pig house disappears. This is because most of the anaerobic bacteria, putrefactive bacteria, and pathogenic bacteria that generate bad smell are eliminated by Bacillus and its metabolites. Furthermore, due to this bad smell, flies are generated in large quantities, and it is normal that the chance of promoting disease transmission is increased.However, the biological material of the present invention contains peptides and metabolites such as polypeptides and cyclic peptides as active ingredients. Therefore, since it has a disinfecting and insecticidal effect, the generation of flies is prevented, and a sanitary stable is improved. As described above, the biological material of the present invention is effective not only in preventing and treating diseases caused by pathogenic bacteria and the like, but also in antistress action and low odor of excrement. In addition, pests such as flies are sharply reduced, do not occur in poultry houses or livestock houses, and do not approach.

The dose of the biological material is 1 mg to 10 mg.
It is effective in the range of 0 mg / day and body weight of 1 kg, preferably 5 to 15 mg / day and body weight of 1 kg, more preferably 8 mg / day and 1 kg of body weight.
１３13 mg / day, body weight 1 kg. If a metabolite or a product such as a peptide separated and purified from a biological material is used instead of the biological material, the dosage may be small. In this case, the mixed culture of Bacillus thuringiensis and Bacillus pumilus-digestion fermentation-elution-separated metabolites and products such as peptides are separated in an amount of 0.04 to 5 m / kg of animal per day.
It is most desirable to mix g with feed. The administration is carried out by directly giving the drug, mixing it into drinking water, or blending it into a feed or a luxury item, but it is generally blended into a feed or the like. It is desirable to administer the drug continuously every day.
Bacillus does not germinate in a dry state (water content of 30% or less) and maintains a spore state. In addition, it only passes through the digestive system in the animal body, and it works against metabolites to attack pathogens and exerts high intestinal regulation, maintaining and promoting animal health, and preventing and treating infectious diseases. It is effective for improving the anti-stress ability. For plant epidemics and pest prevention and control, it is effective to prepare and spray an aqueous solution containing 20 to 500 mg / liter of metabolites and the like.

[0015]

[Example 1] (Example 1) Confirmation of action of biological material to suppress or sterilize pathogenic bacteria Test method: a) Escherichia coli (O157 strain) and b) Salmonella were collected with a platinum loop and inoculated on a flat medium, respectively. ° C
For 3 days in a sterile thermostat. This is the comparison level. In parallel with this, a ′: 0.1 ml of a biological material solution (0.5 g of biological material / 10 ml of sterile water uniformly mixed with a homogenizer) is further dispensed and applied on an E. coli flat medium. 3 at 39 ° C
Cultured in sterile box for days. b ': A biological material solution on a Salmonella flat culture medium
0.1 ml was dispensed and applied, and cultured in a sterile box at 39 ° C. for 3 days. a '': 0.1 ml of a supernatant of a biological material (supernatant obtained by centrifuging a biological material solution at 6000 c / s for 15 minutes: containing only metabolites of bacteria) on an E. coli flat medium And cultured similarly. b '': Supernatant of biological material on Salmonella
0.1 ml was dispensed, applied, and cultured in the same manner. Here, the flat medium was prepared with 0.8% glucose, 0.8% nutrient broth, 0.6% salt, and 1.5% agar. Test results: In a and b, large colonies of Escherichia coli and Salmonella grew, respectively, and covered the entire surface of the flat medium. In a 'and b', colonies of Escherichia coli and Salmonella did not grow on the surface to which the biological material solution was applied, but colonies of Bacillus thuringiensis and Bacillus pumilus were large. In a '' and b '', colonies of Escherichia coli and Salmonella did not occur only in the portions to which the biological material supernatants were applied, and colonies of Escherichia coli and Salmonella greatly proliferated on the remaining surfaces. The above results indicate that Bacillus thuringiensis and Bacillus pumilus, together with the action of their metabolites,
It shows that E. coli and Salmonella were lysed and further digested and propagated as nutrients. Is shown.

(Example 2) Confirmation of action of biological material to control or sterilize pathogenic bacteria Test method: Old small poultry house 2 idle in a large-scale poultry farm
Using the wing (1200 birds each), an appropriate level of biological material administration was determined, and its effect was confirmed. First,
1200 chickens (White Leghorn) in Building A performed chicken raising as before, and this was set as a comparative level. Young chickens in Building B12
00 feathers (white leghorn) were fed a blended feed mixed with a biological material (mixed culture of Bacillus thuringiensis and Bacillus pumilus). The dosage is as follows:
It is as follows. The experiment period is 18 months for both A and B. Level 1: Biological material 1 mg / body weight 1 kg / day Level 2: Biological material 10 mg / body weight 1 kg / day Level 3: Biological material 20 mg / body weight 1 kg / day Level 4: Biological material 30 mg / body weight 1 kg / day Level 5: Biological material 50 mg / Weight 1kg / day Level 6: Biological material 100mg / Weight 1kg / day

Test results: Table 1 shows the average body weight, egg production rate, presence or absence of infectious disease, defeathering, and offensive odor at each level and comparative level of the present invention. As is clear from Table 1, A
In the wing (comparative level), diarrhea was observed, the chickens were also dirty, the feathers were severe, and there was overall skin exposure. The spawning rate also decreased significantly. This is determined to be environmental stress. In addition, large variations in growth are observed. On the other hand, in Building B, there is no disease at Level 2 or higher, no feathers are seen, and chickens are not stained and beautiful. Furthermore, the growth is good and the spawning starts smoothly and the spawning rate is good. In particular, levels 2 and 3 are excellent. Both egg yolk and eggshell are hard. The reason why the eggshell becomes hard is considered to be the result of the improvement of Ca absorption by the action of the peptide contained in the biological material. No fly was found in the poultry house in the experimental plot.

[Table 1]

From the above results, it was revealed that Bacillus in chicken manure decomposes malodorous components, decomposes undigested nutrients, and suppresses or eliminates spoilage bacteria and pathogenic bacteria. In addition, chicken feces were diluted and cultured in the flat medium used in Example 1 and the colonies generated were measured by a phase contrast microscope. Bacillus thuringiensis showed 3 × 10 6
⁸ pcs / gr, Bacillus pumilus 5 × 10 ⁸ pcs / gr
Met. These Bacillus were similar to Bacillus natto, Bifidobacterium and the like without causing any harm to the living body, and the composition of intestinal bacteria in chickens was improved. In addition, it has been found that 10 to 20 mg / kg body weight / day of the biological material is sufficiently effective.

(Example 3) Experiment for confirming the effect on swine raising (action of bacillus against staphylococci) Preliminary investigation: In the case of pigs, there is a terrible infectious disease of swine so far as tonkoro disease. Therefore, the excrement of the pig house affected by an infectious disease accompanied by fever and acute diarrhea was collected, 1 g (wet) thereof was collected, diluted with 100 ml of sterile water, and diluted and cultured as a pathogenic inoculum. This is broth 0.
8%, 0.8% glucose, 0.6% salt, 0.6% agar, 1.5% agar, inoculated on a flat medium to obtain a comparative level, and further, the biological material supernatant used in Example 1, and After applying the biological material solution, the cells were cultured in a sterile box at 39 ° C. for 3 days. Then
While categorizing the colonies that occurred by their characteristics,
Observed with a phase contrast microscope.

The results are shown in Table 2. This is because metabolites contained in biological materials are extremely effective against staphylococci, and the inoculated Bacillus lyses staphylococci and inhibits their growth. Streptococci were also observed in the excrement in the piggery, but streptococci were not observed at the biological material supernatant application level and the biological material solution application level.

[Table 2]

(Example 4) Experiment for confirming the effect on pig raising Test method: Four cages having an area of 30 m ² were prepared, and 10 piglets of 2 months old were placed in each cage. First, the cage of No. 1 was a conventional level cage, and a normal compound feed was given daily for three months. Piglets in No. 2 cages were fed daily with a compound feed supplemented at a rate of 10 mg of biological material / kg of body weight / day for 3 months. Similarly, a mixed feed of 20 mg of biological material / 1 kg of body weight / day was added to piglets of No. 3 cage, and 50 mg of biological material / 1 kg / day of body weight was added to piglets of No. 4 cage. The mixed feed was given daily for three months.

Test results: Table 3 shows the test results.
No infectious disease occurred at the biological material administration level.
The growth of the pig is also good. The same applies to tail biting due to stress.
It was zero. In addition, flies can occur in the piggery
There was no good pig raising environment. In addition, for each cage
Bacterial analysis of excrement was analyzed by dilution culture.
At the comparative level of No. 1, intestinal Vibrus was confirmed, but on the other hand
3 × 10 ^ThreeStopped at No.2 and below are all
No intestinal vibrus bacteria were found, whereas Bacillus sp.
Chiles Turingensis 8 × 10⁸Pieces / gr, bacill
17 × 10⁸/ Gr was detected. Change
And continued observation for 2 months after discontinuation of biological material administration
However, no outbreak of infection was observed. This is immune
It means improvement.

[Table 3]

Example 5 Extraction of Active Ingredients from Biological Material Dilute ethyl alcohol or water is added to a biological material, and the metabolites and products such as peptides, which are active components, are dissolved and extracted by ultrasonication or permeation expansion. This is subjected to solid-liquid separation by centrifugation or membrane filtration, etc., and metabolites and products such as dissolved peptides in the liquid are directly administered to animals and plants in powder form as they are or evaporated to dryness at low temperature, Alternatively, it can be mixed with feed and given, or can be cured by spraying directly on an externally affected area such as a skin disease of animals and plants for controlling bacterial diseases and pests of plants.

In this embodiment, 100 g of each of the biological material treated in the tertiary step and the biological material treated in the digestion step are weighed, 500 ml of ethyl alcohol (1: 3) is added, and the mixture is put into an ultrasonic wave (50 KHz) for elution for 15 minutes. . The eluate is
Store in a separate beaker and re-add 100 ml of ethyl alcohol
l and 100 ml of distilled water, and elute again in an ultrasonic bath. Further, the same operation was repeated and washed with distilled water to make the total volume 1 liter. The eluate is centrifuged (10
(00G × 15 minutes) to obtain a solid-liquid separation to obtain an upper eluate.

Next, 100 ml of the eluate was placed in an evaporating dish, dried in a thermostat at 45 ° C., and metabolites such as peptides and biological materials were quantified as evaporation residues. As a result, the tertiary process treatment was 6.90% and the digestion process treatment was 8.36%. As described above, biological materials contained metabolites and products such as peptides at high concentrations. The eluate was highly transparent and did not contain precipitates or suspended matter, but when acidified by dropping an acid, the protein rapidly solidified and precipitated.
However, it was assumed that the protein that formed a precipitate was relatively large in molecular weight, and that the protein with a small molecular weight did not form a precipitate.However, it was assumed that the protein was dissolved in the eluate. It could be confirmed. Incidentally, the peptide having a small molecular weight was considered to be in a dissolved state.

(Example 6) Maintenance of chicken health and elimination of offensive odor by administration of eluted components In Example 1, it was confirmed that the active ingredient of the biological material sterilized Salmonella. It was clarified that it had a sterilizing effect, and it was conceived to use the effect to prevent or cure bacterial diseases of animals and plants. So Satsuma Chicken 40
In a large chicken house of 00 birds, the health of the chicken was maintained by the diluted eluate, and the bad smell (due to putrefactive bacteria) inherent in the chicken house was improved.

That is, five levels were set for each cage row in the poultry house, and the chickens were given drinking water for three months from the start of breeding. The levels are comparative level (tap water), Experiment 1 (supernatant eluted with 0.5 kg / m ³ of biological material), Experiment 2 (supernatant eluted with 1 kg / m ³ of biological material), Experiment 3 (3 kg of biological material) / M ³ ) and Experiment 4 (supernatant eluted with 5 kg / m ³ of biological material). The elution component concentration in each level of drinking water was 0 ppm in the comparative level, Experiment 1
38 ppm, experiment 2 75 ppm, experiment 3 225 ppm,
Run 4 was 375 ppm. The partition between the comparative level zone and the experimental level zone was set up with vinyl sheets for partition walls.

The results of this experiment are as shown in Table 4;
It has been clarified that eluted components (metabolites and products such as peptides) of biological materials prevent the occurrence of diseases and control insects such as flies to maintain the health of young chickens. In addition, there were severe odors inside and outside the poultry house in the comparative level area, and it was impossible to enter without a protective mask and protective glasses. Furthermore, the outbreak of flies is constantly progressing, and it can be said that there is a great danger inherent in chickens bred in such a poor environment. In contrast, in the experimental plot, there were no flies or odors at all levels, and the chickens were always in good health. From the experimental results, it was confirmed that a high effect was exhibited by giving drinking water containing the dissolved component of 38 ppm or more. In addition, the chicks of the comparative level were vaccinated when bred.

[Table 4]

[0029] were prepared four cages (Example 7) Protection area pig by elution ingredients administered 30 m ^2. Each cage has
As shown in Table 5, piglets having an average body weight of 10.5 kg were introduced and bred for 60 days. For the administration of the eluted components, 10 kg of the biological material was eluted in advance into 100 liters of water, the supernatant water was stored in a tank, and the experimental level was put into a feed and administered daily. In addition, the eluate contains
It contained 6.9 g / l.

The results are as shown in Table 5. It was confirmed that by administering a small amount of the eluted component daily, prevention of infectious disease and improvement of immunity were observed. On the other hand, at the comparative level, the behavior that seems to be due to the stress of the pig became severe, and the appetite declined to fluctuate, the fight between the pigs, the noise level increased, the occurrence of diarrhea symptoms and repeated antibiotic administration, and growth Variation,
And so on. Since no overcrowding stress was observed in the experimental plot, it was found that the eluted components had an anti-stress effect. Streptococci were detected at comparative levels in human waste of swine, but not detected in the experimental plot (diluted culture 10 ² ). In the experimental plot, the bad smell of the piggery was also greatly improved.

[Table 5]

(Example 8) Control and control of plant pests by eluting and dispersing cloth The eluate used in Example 7 is diluted 10-fold with water and sprayed on the plant by a sprayer to control pests and pathogens, thereby improving health. Was recovered. The morning spraying of 1 liter of diluted eluate / 3.3 m ² for 7 days was carried out every morning on the ume forest where aphids and leafworms were outbreaks, and a clear decline of these pests was observed. When sprayed, both the aphids and the snails disappeared and the plum tree recovered its health.

Further, shellworms were attached to perpetual reddish red, and lime sulfuric acid was sprayed three times in winter. A biological material was buried around the root of this perennial, and the diluted eluate was sprayed on the trunk and branches by spraying for 15 days, and both shellworm and anthrax were cured.

In vegetables, in the field where root blight has been established, efforts such as burning field, spraying of fungicides, and spraying of antibiotics are required for disinfection, but it is difficult to cure them. Biological material is sprayed on the root-knotted roots, and the topsoil is mixed with a tiller to mix the biological material. Furthermore, a manure (organic fertilizer) was applied to plant cabbage seedlings. This allows
Spraying of the diluted eluate with a sprayer at a rate of 1 liter / 10 days per 10 m ² was continued until the harvest, and no root blight developed. In addition, a bacterial examination of the soil revealed that the root nodule bacteria had disappeared. Therefore, the alternately planted Chinese cabbage was planted without disinfection, and spraying was performed only twice a month using a spray of the diluted eluate, but no root rot was found. It was also effective when used for exterminating cucumber thrips.

For horticultural use, it is effective for controlling various kinds of pests and disinfecting pathogens. For example, a diluted eluate is sprayed on a Gerbera group that has been destroyed by gerbera black mold disease. Was 100% reproducible. Also,
It also has a bactericidal effect against rose powdery mildew and scab and cures them. In addition, for Saintpaulia, which is said to be difficult to cultivate, spraying the diluted eluate about once a week can eliminate the occurrence of disease and allow it to grow smoothly. It can be said that these phenomena are the result of the active ingredient in the biological material not only killing and killing insects but also improving the immunity of the plant.

[0035]

As described in detail above, the present invention relates to a veterinary drug containing as an active ingredient a metabolite or a product such as a peptide obtained by single or mixed culture of Bacillus thuringiensis and Bacillus pumilus, and Animal feed to which the drug has been added. In addition, the active ingredient (a metabolite or a product such as a peptide) extracted from a biological material is sprayed on a plant to control disease and pests and pathogenic bacteria, and to improve immunity.

This drug has excellent effects on livestock, poultry and other animals in immunostimulation, infectious disease prevention and anti-stress. In this way, all of the excellent effects are exhibited by the action of metabolites and products such as peptides derived from Bacillus, especially Bacillus thuringiensis, Bacillus pumilus, or in combination with the pathogens and spoilage bacteria of Bacillus itself. This is the result of direct attack characteristics. Also,
It has also been clarified that a living body whose immunity has been improved by continuing to administer a biological material or its active ingredient maintains the acquired immunity for a certain period of time.

Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI Theme coat II (reference) A61P 43/00 A61K 31/00 643Q F term (reference) 2B150 AA01 AA05 AB03 AB10 AC02 DC23 DD12 DD26 4C087 AA01 AA02 BC64 CA10 MA02 MA16 MA43 MA52 NA14 ZC61 4H011 AA01 AC01 AC02 BB21 DA02 DA12 DD03 DD04 DE15 DG13

Claims (5)

[Claims] 1. Bacillus thuringiensis (Bacillus t)
huringensis and Bacillus pumil
a biological material for animals, characterized in that it contains metabolites and products such as peptides obtained by mixed culture of the present invention as an active ingredient, and exerts immunostimulation, infection prevention and anti-stress effects in breeding animals. 2. An animal feed to which the biological material according to claim 1 is added. 3. Bacillus thuringiensis and Bacillus.
Metabolites and products are eluted from biological material containing metabolites and products such as peptides obtained by mixed culture of Pumilas as an active ingredient using a solvent such as water or dilute ethanol to form a liquid, or A veterinary drug that is made into a powder form by removing the solvent. 4. An animal feed to which the drug according to claim 3 has been added. 5. Bacillus thuringiensis and Bacillus
Metabolites and products are eluted from biological material containing metabolites and products such as peptides obtained by mixed culture of Pumilas as an active ingredient using a solvent such as water or dilute ethanol to form a liquid, or An agent that prevents solvent, removes powder, and prevents or cures bacterial diseases of plants, or eliminates pests.